AlfalfaGEDB | MsaG005846

M. sativa ssp. caerulea / MsaG005846

KEGG: K18490
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: PF00046.32

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005846	XP_013470433.1	97.727	264	6	0	1	264	1	264	0.0	542

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005846	sp\|Q5QMM3\|WOX8_ORYSJ	53.091	275	103	7	5	262	1	266	2.97e-86	261

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005846	tr\|A0A072VS22\|A0A072VS22_MEDTR	97.727	264	6	0	1	264	1	264	0.0	542

TFs/TRs:

Gene ID	Type	Classification
MsaG005846	TF	HB-WOX

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG005846	MsaG042841	0.802846	7.769843e-49	1.120618e-46
MsaG005846	MsaG046347	0.801966	1.177024e-48	1.663810e-46
MsaG005846	MsaG018616	0.802357	9.789090e-49	1.396219e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005846	MtrunA17_Chr1g0212511	97.727	264	6	0	1	264	1	264	0.0	542
MsaG005846	MtrunA17_Chr3g0144021	69.375	160	44	4	14	168	8	167	2.28e-73	223
MsaG005846	MtrunA17_Chr7g0222981	60.753	186	51	5	5	189	1	165	8.16e-67	205
MsaG005846	MtrunA17_Chr4g0043891	44.706	85	38	1	85	169	8	83	7.81e-17	77.8
MsaG005846	MtrunA17_Chr2g0283141	44.615	65	36	0	83	147	53	117	1.67e-15	75.9
MsaG005846	MtrunA17_Chr7g0223471	42.857	77	40	1	72	144	30	106	1.57e-14	72.8
MsaG005846	MtrunA17_Chr8g0392991	39.506	81	48	1	85	165	96	175	4.62e-13	68.2
MsaG005846	MtrunA17_Chr7g0238431	46.774	62	33	0	85	146	8	69	9.41e-13	65.5
MsaG005846	MtrunA17_Chr4g0031341	46.667	60	32	0	85	144	25	84	7.65e-12	63.9
MsaG005846	MtrunA17_Chr5g0436661	43.333	60	34	0	85	144	33	92	1.10e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG005846	AT4G35550.1	53.383	266	73	6	20	264	33	268	1.88e-85	256
MsaG005846	AT1G20700.3	52.761	163	69	5	10	168	19	177	8.55e-53	171
MsaG005846	AT1G20700.1	52.761	163	69	5	10	168	19	177	1.06e-52	171
MsaG005846	AT1G20710.1	52.857	140	61	4	23	158	13	151	3.59e-34	122
MsaG005846	AT1G20700.2	63.415	82	30	0	75	156	23	104	5.29e-34	120
MsaG005846	AT2G33880.2	44.615	65	36	0	82	146	52	116	3.48e-15	75.1
MsaG005846	AT5G45980.1	44.615	65	36	0	82	146	52	116	3.61e-15	74.3
MsaG005846	AT2G33880.1	45.312	64	35	0	83	146	53	116	3.73e-15	74.7
MsaG005846	AT3G18010.1	46.667	60	32	0	85	144	76	135	2.88e-13	69.3
MsaG005846	AT3G11260.1	48.333	60	31	0	85	144	24	83	1.31e-12	65.1
MsaG005846	AT5G05770.1	37.662	77	48	0	85	161	29	105	1.63e-12	63.5
MsaG005846	AT2G28610.1	43.548	62	35	0	85	146	8	69	4.89e-12	64.7
MsaG005846	AT2G01500.1	45.455	55	30	0	85	139	61	115	4.84e-11	62.0

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACATTTGTTTCAGAAATT+TGG	0.112745	1:-85850564	None:intergenic
TTGATCTAGCACGCCTATTT+TGG	0.146776	1:-85850597	None:intergenic
TTTATAATTGGTTCCAAAAT+AGG	0.153266	1:+85850584	MsaT005846.1:CDS
CTGAAACAAATGTTTATAAT+TGG	0.206106	1:+85850572	MsaT005846.1:CDS
AGACATGCCACTCAAATTTC+TGG	0.262992	1:-85850854	None:intergenic
CCTTTCATCAGGGGTTAGGC+TGG	0.295215	1:+85850369	MsaT005846.1:intron
CGAATATACGCTCAAGAATC+TGG	0.304326	1:-85850474	None:intergenic
AATTGTAGTCCTCTGCTTGC+TGG	0.362368	1:-85851469	None:intergenic
CGTATATTCGATCAGGGAAA+TGG	0.392284	1:+85850487	MsaT005846.1:CDS
GCAAGCAGAGGACTACAATT+TGG	0.393799	1:+85851472	MsaT005846.1:CDS
TTTCATCAGGGGTTAGGCTG+GGG	0.399266	1:+85850371	MsaT005846.1:intron
TCTTGAGCGTATATTCGATC+AGG	0.413169	1:+85850480	MsaT005846.1:CDS
CTTTCATCAGGGGTTAGGCT+GGG	0.414371	1:+85850370	MsaT005846.1:intron
TCAAGAATCTGGAGCTGAAC+CGG	0.421307	1:-85850463	None:intergenic
TTTCAGAAATTTGGCCATGC+TGG	0.437267	1:-85850555	None:intergenic
TGTAGGAGTCCACCTCTGTC+TGG	0.437314	1:-85850440	None:intergenic
GGTGATGACTGATGAACAAT+TGG	0.449180	1:+85848888	MsaT005846.1:CDS
TCTCAACAAAATGCTGCTCT+AGG	0.464326	1:+85850718	MsaT005846.1:CDS
TCTGCTCAACAAGATCTTGC+TGG	0.471131	1:+85848982	MsaT005846.1:CDS
TGTTGAGAATGAAATTCCTC+TGG	0.474708	1:-85850703	None:intergenic
CTCAACAAGCTGTTCACAAA+TGG	0.485586	1:-85848944	None:intergenic
TCAGGGAATGACTATCTAGC+TGG	0.485659	1:+85851413	MsaT005846.1:intron
ACAGAGGTGGACTCCTACAC+CGG	0.495315	1:+85850444	MsaT005846.1:CDS
CTCAACAAAATGCTGCTCTA+GGG	0.501777	1:+85850719	MsaT005846.1:CDS
TGTCGAAGCGATGTATGTTA+AGG	0.503782	1:+85848867	MsaT005846.1:CDS
AGAAACAACAAGTACAGAAT+GGG	0.518129	1:+85848842	MsaT005846.1:CDS
AGAGAAAATGGTGAACATGA+TGG	0.521726	1:+85848813	None:intergenic
GCAGAGGACTACAATTTGGC+AGG	0.523975	1:+85851476	MsaT005846.1:CDS
TGCGACTCATTGATGACAGC+TGG	0.529717	1:+85850403	MsaT005846.1:CDS
CAGAAACAACAAGTACAGAA+TGG	0.532343	1:+85848841	MsaT005846.1:CDS
AACATATTCCGTGTTTCCAT+CGG	0.539020	1:+85850813	MsaT005846.1:CDS
ACCGCCATCCGATGGAAACA+CGG	0.553887	1:-85850821	None:intergenic
AGAGACTGAGGTTGATTCCA+AGG	0.556104	1:+85850666	MsaT005846.1:CDS
GATCTTATACCGCCATCCGA+TGG	0.574800	1:-85850829	None:intergenic
CTTGAGCGTATATTCGATCA+GGG	0.579984	1:+85850481	MsaT005846.1:CDS
ATCTGGAGCTGAACCGGTGT+AGG	0.590506	1:-85850457	None:intergenic
TCCGTGTTTCCATCGGATGG+CGG	0.595864	1:+85850820	MsaT005846.1:CDS
TATTCCGTGTTTCCATCGGA+TGG	0.597599	1:+85850817	MsaT005846.1:CDS
GCTACTGAACTCAGCCAGCA+TGG	0.597739	1:+85850541	MsaT005846.1:CDS
TCAACAAAATGCTGCTCTAG+GGG	0.599937	1:+85850720	MsaT005846.1:CDS
AACTAGTAACACTGAATCAG+AGG	0.600354	1:+85850642	MsaT005846.1:CDS
CATGCCACTCAAATTTCTGG+TGG	0.605707	1:-85850851	None:intergenic
AAATGGTGAACATGATGGAA+TGG	0.621193	1:+85848818	None:intergenic
AAATCACATCCAGACAGAGG+TGG	0.644627	1:+85850431	MsaT005846.1:CDS
ATGAACAATTGGAAACTCTG+AGG	0.682721	1:+85848899	MsaT005846.1:CDS
TGAATCAGAGGTAGAGACTG+AGG	0.683491	1:+85850654	MsaT005846.1:CDS
AGATCCACCAGAAATTTGAG+TGG	0.687166	1:+85850847	MsaT005846.1:CDS
GGATAAGAAGACAAAACCAG+AGG	0.688078	1:+85850687	MsaT005846.1:CDS
CAATATATACCAGCAAGCAG+AGG	0.697674	1:+85851460	MsaT005846.1:CDS
ACAAAATCACATCCAGACAG+AGG	0.711170	1:+85850428	MsaT005846.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	85848820	85851502	85848820	ID=MsaG005846
Chr1	mRNA	85848820	85851502	85848820	ID=MsaT005846.1;Parent=MsaG005846
Chr1	exon	85848820	85849003	85848820	ID=MsaT005846.1.exon1;Parent=MsaT005846.1
Chr1	CDS	85848820	85849003	85848820	ID=cds.MsaT005846.1;Parent=MsaT005846.1
Chr1	exon	85850380	85850904	85850380	ID=MsaT005846.1.exon2;Parent=MsaT005846.1
Chr1	CDS	85850380	85850904	85850380	ID=cds.MsaT005846.1;Parent=MsaT005846.1
Chr1	exon	85851417	85851502	85851417	ID=MsaT005846.1.exon3;Parent=MsaT005846.1
Chr1	CDS	85851417	85851502	85851417	ID=cds.MsaT005846.1;Parent=MsaT005846.1

Gene Sequence

>MsaT005846.1

ATGGTGAACATGATGGAATGGCAGAAACAACAAGTACAGAATGGGAATGTCGAAGCGATGTATGTTAAGGTGATGACTGATGAACAATTGGAAACTCTGAGGAAACAAATTGCTGTTTATGCTACCATTTGTGAACAGCTTGTTGAGATGCATAAAACTCTCTCTGCTCAACAAGATCTTGCTGGGGTTAGGCTGGGGAATTTATACTGCGACTCATTGATGACAGCTGGTCACAAAATCACATCCAGACAGAGGTGGACTCCTACACCGGTTCAGCTCCAGATTCTTGAGCGTATATTCGATCAGGGAAATGGAACTCCAAGCAAAGAAAAAATCAAGGAGATTGCTACTGAACTCAGCCAGCATGGCCAAATTTCTGAAACAAATGTTTATAATTGGTTCCAAAATAGGCGTGCTAGATCAAAAAGAAAAATGCAAAATGGGGGAACTAGTAACACTGAATCAGAGGTAGAGACTGAGGTTGATTCCAAGGATAAGAAGACAAAACCAGAGGAATTTCATTCTCAACAAAATGCTGCTCTAGGGGATGAAAATTTGTGCTACCAGAACCAAGAAAAATGCTCTGAATTGCAGTACTTGAATCATGATTCTAACAAAACATATTCCGTGTTTCCATCGGATGGCGGTATAAGATCCACCAGAAATTTGAGTGGCATGTCTCTTTATGATGAAGTGCTATCAAACTCAAGGAATGACTATCTAGCTGGAAAAATGGAAGTATCTGGGAGTTACAATATATACCAGCAAGCAGAGGACTACAATTTGGCAGGTTGA

Protein sequence

>MsaT005846.1

MVNMMEWQKQQVQNGNVEAMYVKVMTDEQLETLRKQIAVYATICEQLVEMHKTLSAQQDLAGVRLGNLYCDSLMTAGHKITSRQRWTPTPVQLQILERIFDQGNGTPSKEKIKEIATELSQHGQISETNVYNWFQNRRARSKRKMQNGGTSNTESEVETEVDSKDKKTKPEEFHSQQNAALGDENLCYQNQEKCSELQYLNHDSNKTYSVFPSDGGIRSTRNLSGMSLYDEVLSNSRNDYLAGKMEVSGSYNIYQQAEDYNLAG*