AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005915

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005915 MtrunA17_Chr1g0213321 98.682 683 0 3 1 681 1 676 0.0 1392
MsaG005915 MtrunA17_Chr7g0214531 85.572 603 78 2 84 681 1 599 0.0 1105
MsaG005915 MtrunA17_Chr1g0146301 71.342 663 179 4 22 680 28 683 0.0 995
MsaG005915 MtrunA17_Chr7g0253211 66.528 720 190 5 1 681 178 885 0.0 984
MsaG005915 MtrunA17_Chr3g0140431 75.125 599 142 3 86 680 2 597 0.0 969
MsaG005915 MtrunA17_Chr8g0340491 70.439 592 165 4 96 680 102 690 0.0 881
MsaG005915 MtrunA17_Chr4g0071031 37.770 278 161 5 124 398 9 277 1.07e-57 202
MsaG005915 MtrunA17_Chr2g0329981 37.770 278 161 5 124 398 9 277 1.38e-57 202
MsaG005915 MtrunA17_Chr1g0166821 38.028 284 162 6 118 398 6 278 1.23e-56 199
MsaG005915 MtrunA17_Chr8g0342821 37.956 274 157 6 124 394 9 272 7.50e-56 197
MsaG005915 MtrunA17_Chr2g0299661 37.050 278 163 5 124 398 9 277 8.17e-56 197
MsaG005915 MtrunA17_Chr7g0261501 37.050 278 163 5 124 398 9 277 1.32e-55 196
MsaG005915 MtrunA17_Chr8g0390211 38.408 289 161 8 114 398 2 277 1.36e-54 192
MsaG005915 MtrunA17_Chr1g0187571 37.676 284 163 6 118 398 6 278 1.92e-54 193
MsaG005915 MtrunA17_Chr6g0486611 37.818 275 157 6 124 394 9 273 4.44e-54 191
MsaG005915 MtrunA17_Chr1g0146151 37.500 280 161 6 118 394 5 273 4.75e-54 191
MsaG005915 MtrunA17_Chr1g0183631 36.268 284 167 6 118 398 5 277 1.65e-53 190
MsaG005915 MtrunA17_Chr3g0140071 35.000 280 168 6 118 394 5 273 1.60e-51 184
MsaG005915 MtrunA17_Chr1g0167261 36.620 284 164 6 116 394 4 276 4.55e-51 181
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005915 AT3G13670.1 73.538 684 159 6 9 680 29 702 0.0 1046
MsaG005915 AT5G18190.2 66.619 701 204 7 1 681 1 691 0.0 969
MsaG005915 AT5G18190.1 66.619 701 204 7 1 681 1 691 0.0 969
MsaG005915 AT2G25760.1 65.512 693 205 7 1 680 1 672 0.0 967
MsaG005915 AT3G03940.1 65.223 716 199 7 1 681 1 701 0.0 964
MsaG005915 AT2G25760.2 65.374 696 204 9 1 680 1 675 0.0 961
MsaG005915 AT3G03930.1 65.833 240 82 0 441 680 48 287 2.24e-117 353
MsaG005915 AT4G28860.2 39.100 289 159 8 114 398 2 277 6.85e-58 201
MsaG005915 AT4G28860.1 39.100 289 159 8 114 398 2 277 2.58e-57 200
MsaG005915 AT4G28880.3 39.100 289 159 8 114 398 2 277 5.35e-57 199
MsaG005915 AT4G28880.2 39.100 289 159 8 114 398 2 277 5.35e-57 199
MsaG005915 AT4G28880.1 39.100 289 159 8 114 398 2 277 5.35e-57 199
MsaG005915 AT4G28880.4 39.100 289 159 8 114 398 2 277 5.35e-57 199
MsaG005915 AT2G19470.1 37.410 278 162 5 124 398 9 277 1.15e-56 199
MsaG005915 AT3G23340.1 38.028 284 162 6 118 398 5 277 1.67e-56 199
MsaG005915 AT4G14340.2 38.380 284 161 6 118 398 11 283 2.63e-56 196
MsaG005915 AT4G14340.1 38.380 284 161 6 118 398 11 283 3.66e-56 198
MsaG005915 AT4G26100.1 36.331 278 165 5 124 398 9 277 7.62e-56 197
MsaG005915 AT5G44100.1 37.324 284 164 6 118 398 5 277 8.15e-56 197
MsaG005915 AT5G44100.2 37.324 284 164 6 118 398 5 277 8.15e-56 197
MsaG005915 AT1G04440.1 38.380 284 161 6 118 398 5 277 1.67e-55 197
MsaG005915 AT1G03930.1 36.268 284 167 6 118 398 5 277 2.36e-55 196
MsaG005915 AT5G43320.1 37.676 284 163 6 118 398 5 277 3.82e-55 196
MsaG005915 AT5G57015.1 35.971 278 166 5 124 398 9 277 9.62e-55 194
MsaG005915 AT5G43320.2 37.676 284 163 6 118 398 5 277 1.45e-54 191
MsaG005915 AT5G43320.3 37.676 284 163 6 118 398 5 277 1.45e-54 191
MsaG005915 AT4G28540.1 36.620 284 166 6 118 398 9 281 2.08e-54 194
MsaG005915 AT1G72710.1 36.331 278 164 6 124 398 9 276 2.65e-54 193
MsaG005915 AT3G23340.2 38.431 255 149 4 147 398 14 263 9.39e-51 182
MsaG005915 AT4G08800.1 31.655 278 146 6 124 398 9 245 4.92e-39 146

Find 196 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCATTATCCCAACGTTTA+TGG 0.104765 1:-86597432 None:intergenic
TTCAATTACTATGTTCTCTT+TGG 0.172008 1:-86592441 None:intergenic
GTCTCGAGAAATTGTTTAAA+AGG 0.213743 1:-86595217 None:intergenic
CTACCTCTGGTGGCCGGCTT+GGG 0.245833 1:-86592314 None:intergenic
CTTCCTTTATAGTGTACTTT+AGG 0.251429 1:-86593382 None:intergenic
AAGTAACTAAGTACCCTTTC+TGG 0.258093 1:-86592587 None:intergenic
GGATGATTTGTTGATGTTCT+TGG 0.261556 1:-86592360 None:intergenic
GGCGGCGGCTAATCTAGTTC+TGG 0.265053 1:-86592339 None:intergenic
TGAGCACCTTCAGTATTAAT+TGG 0.274374 1:-86595331 None:intergenic
TTCTACATTACTTCTATTGC+AGG 0.285204 1:+86596022 MsaT005915.1:CDS
GCTATACATGAGACCATTTC+AGG 0.290608 1:-86594307 None:intergenic
GCGCTTTACCTTTGTTTCAT+TGG 0.295615 1:-86595588 None:intergenic
GCCGGCTTGGGTGGCGGAGG+AGG 0.296043 1:-86592302 None:intergenic
TTTCTTGTTGACCTTGGTTT+AGG 0.298294 1:+86594602 MsaT005915.1:CDS
CTTCTTGTTTATCCATTTGT+AGG 0.299475 1:-86597206 None:intergenic
TCTACCTCTGGTGGCCGGCT+TGG 0.302451 1:-86592315 None:intergenic
ATACACTAATCTTCCTTCAT+AGG 0.302617 1:+86594998 MsaT005915.1:CDS
TGCTAGCGTTCATGCTCATT+TGG 0.309315 1:+86594933 MsaT005915.1:CDS
CACAACAGAGCTTCCATTAC+TGG 0.311269 1:-86596047 None:intergenic
TTCTTCTCCTGTGCTGTGTT+GGG 0.314845 1:-86594575 None:intergenic
CAATCTCTGGGCACTTATAA+TGG 0.319388 1:+86595823 MsaT005915.1:CDS
ATCAACGCCCCGACATGTTT+AGG 0.321666 1:+86594767 MsaT005915.1:CDS
AACAGTAAAGGTTGCAACTA+TGG 0.325296 1:+86592981 MsaT005915.1:CDS
TATCATGTTGTTCCAATCTC+TGG 0.326597 1:+86595810 MsaT005915.1:CDS
TCAATTACTATGTTCTCTTT+GGG 0.327798 1:-86592440 None:intergenic
ATGCAGGTTATGGACATTCT+TGG 0.329274 1:+86593499 MsaT005915.1:intron
ACGGCGGTGGGTCCTCCTCC+TGG 0.337831 1:-86592382 None:intergenic
ATCCATAAACGTTGGGATAA+TGG 0.338559 1:+86597430 MsaT005915.1:CDS
ATTGGCCGCAATGCAGGATT+AGG 0.338641 1:-86595313 None:intergenic
AAGCTAATATCCTTGTTTGA+TGG 0.338803 1:+86595280 MsaT005915.1:CDS
AAACTTGGTAAAGGTGGATT+TGG 0.339810 1:+86592787 MsaT005915.1:CDS
CTGTGCGCCCATAGCACAAA+TGG 0.345836 1:-86597695 None:intergenic
ATCTTGCCCATTTGTGCTAT+GGG 0.356482 1:+86597688 MsaT005915.1:CDS
AGCACAAATGGGCAAGATAA+AGG 0.360045 1:-86597683 None:intergenic
TCTCCTGTGCTGTGTTGGGC+TGG 0.366766 1:-86594571 None:intergenic
TTTCTTCTCCTGTGCTGTGT+TGG 0.372909 1:-86594576 None:intergenic
CATCACCAGGTCTGTTCCTC+GGG 0.373424 1:-86597510 None:intergenic
TGGGAAGAACTGCCAGTAGA+AGG 0.375365 1:+86594953 MsaT005915.1:CDS
GATATTAGCTTGGAATAGTT+AGG 0.378909 1:-86595268 None:intergenic
GTGGCCGGCTTGGGTGGCGG+AGG 0.380088 1:-86592305 None:intergenic
TATCTTGCCCATTTGTGCTA+TGG 0.384054 1:+86597687 MsaT005915.1:CDS
TTCTTCGCAGGAATGGATCT+TGG 0.387340 1:+86595982 MsaT005915.1:intron
CTTGAAAAGTTGCATGCAAA+AGG 0.389023 1:+86594349 MsaT005915.1:CDS
CCTCTGGTGGCCGGCTTGGG+TGG 0.397147 1:-86592311 None:intergenic
CATTATAAGTGCCCAGAGAT+TGG 0.398095 1:-86595822 None:intergenic
ATACCTAAAGTACACTATAA+AGG 0.399245 1:+86593379 MsaT005915.1:CDS
CTGGTGGCCGGCTTGGGTGG+CGG 0.399453 1:-86592308 None:intergenic
GCTAGCGTTCATGCTCATTT+GGG 0.400502 1:+86594934 MsaT005915.1:CDS
TTCCTTTCTACCTTGTATAG+AGG 0.401858 1:-86592766 None:intergenic
ATTTCCCAGCGCTCATGTTA+AGG 0.404732 1:+86597561 MsaT005915.1:CDS
GTTTAGGATGTCTCCTGAAA+TGG 0.410442 1:+86594294 MsaT005915.1:intron
GTCGGGGCGTTGATCGTATT+CGG 0.415725 1:-86594758 None:intergenic
GTCCAAGTTTGTGGGATGTA+TGG 0.415770 1:+86593521 MsaT005915.1:CDS
AACGTTGGGATAATGGTTAT+AGG 0.420827 1:+86597437 MsaT005915.1:CDS
TTCTTGTTTATCCATTTGTA+GGG 0.425761 1:-86597205 None:intergenic
GGTTCAACAACGAAAGTATC+AGG 0.427084 1:-86592416 None:intergenic
GGAGAAGGAGCAGCAGGACG+AGG 0.428945 1:-86592281 None:intergenic
GGATGCTGGAACTAATTATT+CGG 0.431973 1:+86595844 MsaT005915.1:CDS
AAAGAAAACCTAAACATGTC+GGG 0.437751 1:-86594775 None:intergenic
TCATCACCAGGTCTGTTCCT+CGG 0.440307 1:-86597511 None:intergenic
ACTGAAATCCATTGCGAAGC+AGG 0.440885 1:-86595549 None:intergenic
GTCACCTCAATGGGTACTGC+TGG 0.441323 1:+86597247 MsaT005915.1:CDS
TTGGTAGGTTGGGCAAAAGC+GGG 0.446903 1:+86595464 MsaT005915.1:intron
GCGGCGGCTAATCTAGTTCT+GGG 0.447476 1:-86592338 None:intergenic
ACATTCTTGGTCCAAGTTTG+TGG 0.448210 1:+86593512 MsaT005915.1:CDS
TACACTAATCTTCCTTCATA+GGG 0.452878 1:+86594999 MsaT005915.1:CDS
TTCCATACATCCCACAAACT+TGG 0.453446 1:-86593523 None:intergenic
TGGGTACTGCTGGAAATCGC+TGG 0.454321 1:+86597257 MsaT005915.1:CDS
GTATTAATTGGCCGCAATGC+AGG 0.457850 1:-86595319 None:intergenic
AGAAATTGTTTAAAAGGAGC+GGG 0.458134 1:-86595211 None:intergenic
CGTTTATGGATGCCTTCACT+TGG 0.459779 1:-86597418 None:intergenic
TAATACTGAAGGTGCTCAAA+AGG 0.460450 1:+86595336 MsaT005915.1:CDS
GCGAATCTTTCCCCTACAAA+TGG 0.461534 1:+86597194 MsaT005915.1:CDS
TCTAGTTCTGGGTCTACCTC+TGG 0.461838 1:-86592327 None:intergenic
TACTTTGTCTATTAGGGTGA+TGG 0.463887 1:+86595124 MsaT005915.1:intron
GGAATGGATCTTGGAACAAT+GGG 0.467089 1:+86595991 MsaT005915.1:intron
GGAGGAGGAGAAGGAGCAGC+AGG 0.469679 1:-86592287 None:intergenic
GGAGAGTTGGCATCTTCCTC+GGG 0.472733 1:-86592557 None:intergenic
CGCCTCTATACAAGGTAGAA+AGG 0.473354 1:+86592764 MsaT005915.1:CDS
AAACATACCTCGACGGCTCC+TGG 0.474817 1:-86592847 None:intergenic
TCCTCCTCCGCCACCCAAGC+CGG 0.474980 1:+86592301 MsaT005915.1:CDS
TTGTATACTTGCCACTCATA+AGG 0.480559 1:-86593008 None:intergenic
CAACGCTGGATTCAGTGATC+AGG 0.480740 1:+86597297 MsaT005915.1:CDS
CATTCTTGGTCCAAGTTTGT+GGG 0.483498 1:+86593513 MsaT005915.1:CDS
TTGGGTGGCGGAGGAGGAGA+AGG 0.483624 1:-86592296 None:intergenic
CCCTACCTTAACATGAGCGC+TGG 0.483998 1:-86597566 None:intergenic
TACAGCAACTGCAGCTACAT+TGG 0.486190 1:+86597462 MsaT005915.1:CDS
TGGAGAGTTGGCATCTTCCT+CGG 0.487318 1:-86592558 None:intergenic
CTCTCCACCTCTTCCAGAAA+GGG 0.488654 1:+86592574 MsaT005915.1:CDS
CGGCGGTGGGTCCTCCTCCT+GGG 0.489758 1:-86592381 None:intergenic
CTAAGTACCCTTTCTGGAAG+AGG 0.498411 1:-86592581 None:intergenic
AGCACCCTTGGTGGTAGTCA+TGG 0.498708 1:+86593355 MsaT005915.1:intron
TCCTTGTTTGATGGTATGCT+CGG 0.499312 1:+86595289 MsaT005915.1:CDS
GAGAAATTGTTTAAAAGGAG+CGG 0.500852 1:-86595212 None:intergenic
ATCATGTTGTTCCAATCTCT+GGG 0.505022 1:+86595811 MsaT005915.1:CDS
ATTTAATTTCTGCAGGTACA+AGG 0.506190 1:+86597146 MsaT005915.1:intron
ATCAAACAAGGATATTAGCT+TGG 0.507292 1:-86595278 None:intergenic
TACAAGGTAGAAAGGAAACT+TGG 0.508090 1:+86592772 MsaT005915.1:CDS
GAAAGGAAACTTGGTAAAGG+TGG 0.508224 1:+86592781 MsaT005915.1:CDS
AAATTGTTTCTTGTTGACCT+TGG 0.513818 1:+86594596 MsaT005915.1:CDS
GGGTACTGCTGGAAATCGCT+GGG 0.513888 1:+86597258 MsaT005915.1:CDS
TGAAGGCATCCATAAACGTT+GGG 0.514683 1:+86597423 MsaT005915.1:CDS
TGGTAAAGGTGGATTTGGCC+AGG 0.515374 1:+86592792 MsaT005915.1:CDS
ACTCTCCACCTCTTCCAGAA+AGG 0.515626 1:+86592573 MsaT005915.1:CDS
ATTACTATGTTCTCTTTGGG+AGG 0.521147 1:-86592437 None:intergenic
TTCCATCAGCCACATTGTAA+TGG 0.521580 1:-86595733 None:intergenic
TTCAAGATCATCCCTTCTAC+TGG 0.523197 1:-86594965 None:intergenic
CGAAGAAGCTGTTGGTGTGA+TGG 0.523291 1:+86592481 MsaT005915.1:CDS
GTTTCCTGAGTTTCATCACC+AGG 0.525100 1:-86597523 None:intergenic
GTTCATTATAGTTATGTACA+TGG 0.527173 1:+86594518 MsaT005915.1:intron
CAGGGATTCCTGCTTCGCAA+TGG 0.528640 1:+86595541 MsaT005915.1:CDS
TCTATTGCAGGGGCCAGTAA+TGG 0.529630 1:+86596034 MsaT005915.1:CDS
ATTGTTATGTCTCGCAACGC+TGG 0.532120 1:+86597283 MsaT005915.1:CDS
TACCATTACAATGTGGCTGA+TGG 0.532605 1:+86595731 MsaT005915.1:CDS
TCTACATTACTTCTATTGCA+GGG 0.533747 1:+86596023 MsaT005915.1:CDS
ACAAATGGATAAACAAGAAG+TGG 0.535275 1:+86597209 MsaT005915.1:CDS
ATGGCAATCGACCCCTATGA+AGG 0.535810 1:-86595011 None:intergenic
TGTTGTGTCAAAAGCACCCT+TGG 0.537417 1:+86593343 MsaT005915.1:intron
AGGAATGGATCTTGGAACAA+TGG 0.539816 1:+86595990 MsaT005915.1:intron
TCTGTTGTGGTGATGTCAAA+AGG 0.540053 1:+86596061 MsaT005915.1:CDS
TTCTGGAAGAGGTGGAGAGT+TGG 0.541195 1:-86592570 None:intergenic
TCTCAGTGCTAATAAAGATA+AGG 0.542097 1:+86592520 MsaT005915.1:CDS
CGTGAGCGTGCAAATGCACC+AGG 0.542739 1:+86592829 MsaT005915.1:CDS
CTGGGTCTACCTCTGGTGGC+CGG 0.543507 1:-86592320 None:intergenic
GGTTGTAATAACAACGGCGG+TGG 0.545020 1:-86592395 None:intergenic
ATAAACAAGAAGTGGAAAGA+AGG 0.546623 1:+86597217 MsaT005915.1:CDS
TGATTTGTTGATGTTCTTGG+CGG 0.547100 1:-86592357 None:intergenic
TAAAGAAAACCTAAACATGT+CGG 0.548421 1:-86594776 None:intergenic
TTGAAAAGTTGCATGCAAAA+GGG 0.550394 1:+86594350 MsaT005915.1:CDS
TTTGAACATAGAAACAGTAA+AGG 0.551586 1:+86592969 MsaT005915.1:CDS
TGGGCACTTATAATGGATGC+TGG 0.551892 1:+86595830 MsaT005915.1:CDS
TGTGCGCCCATAGCACAAAT+GGG 0.560138 1:-86597694 None:intergenic
TTTGGTAGGTTGGGCAAAAG+CGG 0.560454 1:+86595463 MsaT005915.1:intron
TGGGATGTATGGAATACCTC+AGG 0.561689 1:+86593532 MsaT005915.1:CDS
TTCATAGGGGTCGATTGCCA+TGG 0.565345 1:+86595013 MsaT005915.1:CDS
ATGATACACAGCTAATAAGA+AGG 0.568771 1:-86595793 None:intergenic
TTTGTTGATGTTCTTGGCGG+CGG 0.570338 1:-86592354 None:intergenic
AAGTGGAAAGATTCGTCAAG+TGG 0.575587 1:+86594727 MsaT005915.1:CDS
CATAACTCACAGCAACAAAG+TGG 0.578338 1:+86594710 MsaT005915.1:intron
AGGAGGATCGCCTCTATACA+AGG 0.578673 1:+86592756 MsaT005915.1:CDS
GGCTGTTGCTGCTGTTCCCG+AGG 0.579260 1:+86592541 MsaT005915.1:CDS
GGGAAGAACTGCCAGTAGAA+GGG 0.581850 1:+86594954 MsaT005915.1:CDS
GTAGAAAGGAAACTTGGTAA+AGG 0.586481 1:+86592778 MsaT005915.1:CDS
GAAGAAGCTGTTGGTGTGAT+GGG 0.588720 1:+86592482 MsaT005915.1:CDS
ATTTCCAGCAGTACCCATTG+AGG 0.592136 1:-86597251 None:intergenic
AGGGGTCGATTGCCATGGCA+AGG 0.592938 1:+86595018 MsaT005915.1:CDS
AGGCCAGCCCAACACAGCAC+AGG 0.593138 1:+86594568 MsaT005915.1:CDS
ACAAGGAGAATATTATGTGA+TGG 0.595744 1:+86593405 MsaT005915.1:CDS
CTACATTACTTCTATTGCAG+GGG 0.596116 1:+86596024 MsaT005915.1:CDS
CCACCCAAGCCGGCCACCAG+AGG 0.596199 1:+86592311 MsaT005915.1:CDS
GTGAAGGCATCCATAAACGT+TGG 0.598118 1:+86597422 MsaT005915.1:CDS
CAGACCTGGTGATGAAACTC+AGG 0.603132 1:+86597519 MsaT005915.1:CDS
CAACAAATCATCCCAGGAGG+AGG 0.604886 1:+86592370 MsaT005915.1:CDS
GAACATCAACAAATCATCCC+AGG 0.605407 1:+86592364 MsaT005915.1:CDS
TATACTTGCCACTCATAAGG+AGG 0.605547 1:-86593005 None:intergenic
TAGCACAGCATATTGAGAGA+GGG 0.605693 1:+86595759 MsaT005915.1:CDS
CCTACCTTAACATGAGCGCT+GGG 0.608481 1:-86597565 None:intergenic
TGGTAGGTTGGGCAAAAGCG+GGG 0.609732 1:+86595465 MsaT005915.1:intron
TTGCGGCCAATTAATACTGA+AGG 0.613238 1:+86595325 MsaT005915.1:CDS
AGTACCCTTTCTGGAAGAGG+TGG 0.614975 1:-86592578 None:intergenic
GAAGAAGACGAAGAAGCTGT+TGG 0.615462 1:+86592473 MsaT005915.1:CDS
TCTTGTTTATCCATTTGTAG+GGG 0.617352 1:-86597204 None:intergenic
CGCTTATATTAAGCATCCCG+AGG 0.618789 1:+86597494 MsaT005915.1:CDS
TAAACAATTTCTCGAGACTG+TGG 0.620421 1:+86595222 MsaT005915.1:CDS
CTAGACAACCAATGAAACAA+AGG 0.626026 1:+86595580 MsaT005915.1:CDS
AGTTCTGGGTCTACCTCTGG+TGG 0.628657 1:-86592324 None:intergenic
TTAGCACAGCATATTGAGAG+AGG 0.629146 1:+86595758 MsaT005915.1:CDS
CAGTAATGGAAGCTCTGTTG+TGG 0.630181 1:+86596048 MsaT005915.1:CDS
TGTGTCAAAAGCACCCTTGG+TGG 0.632127 1:+86593346 MsaT005915.1:intron
GTTGTAATAACAACGGCGGT+GGG 0.636640 1:-86592394 None:intergenic
TATTCCATGACTACCACCAA+GGG 0.640889 1:-86593359 None:intergenic
AGCATCCCGAGGAACAGACC+TGG 0.642592 1:+86597505 MsaT005915.1:CDS
AAGAAAACCTAAACATGTCG+GGG 0.643052 1:-86594774 None:intergenic
GAAATTGTTTAAAAGGAGCG+GGG 0.650034 1:-86595210 None:intergenic
AAATGCACCAGGAGCCGTCG+AGG 0.664220 1:+86592840 MsaT005915.1:CDS
GTACACTATAAAGGAAGACA+AGG 0.671240 1:+86593388 MsaT005915.1:CDS
GCGGGGTAGATTGAATGTTG+AGG 0.672164 1:+86595482 MsaT005915.1:CDS
ACTATGGCCCTCCTTATGAG+TGG 0.673706 1:+86592997 MsaT005915.1:CDS
AGAATGCCTCAGCTTCGTAG+TGG 0.674178 1:+86592248 None:intergenic
ATACTTGCCACTCATAAGGA+GGG 0.674859 1:-86593004 None:intergenic
TCCGAGCATACCATCAAACA+AGG 0.675450 1:-86595290 None:intergenic
AAAAGAGAAACATACCTCGA+CGG 0.677481 1:-86592854 None:intergenic
ACACTAATCTTCCTTCATAG+GGG 0.679283 1:+86595000 MsaT005915.1:CDS
GTGCATTTGCACGCTCACGA+CGG 0.682084 1:-86592825 None:intergenic
TTATACTCACGACTGGCCTG+AGG 0.682870 1:-86593548 None:intergenic
GTATCAGGTTGTAATAACAA+CGG 0.689192 1:-86592401 None:intergenic
CGAACACCACTACGAAGCTG+AGG 0.690566 1:-86592254 None:intergenic
GCATCCACTAACCTAAACCA+AGG 0.698911 1:-86594613 None:intergenic
TGTACCTGAAAACCTTGCCA+TGG 0.702475 1:-86595030 None:intergenic
GTATTCCATGACTACCACCA+AGG 0.709926 1:-86593360 None:intergenic
GTGATGGGTGATGAAAGAGT+TGG 0.715379 1:+86592497 MsaT005915.1:CDS
GGTACTGCTGGAAATCGCTG+GGG 0.715640 1:+86597259 MsaT005915.1:CDS
CATCAACAAATCATCCCAGG+AGG 0.739128 1:+86592367 MsaT005915.1:CDS
TCAGGTTGTAATAACAACGG+CGG 0.757049 1:-86592398 None:intergenic
TCGGACCTAATCCTGCATTG+CGG 0.788069 1:+86595308 MsaT005915.1:CDS
TTGTAGGTACCATTACAATG+TGG 0.824443 1:+86595724 MsaT005915.1:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr1 gene 86592251 86597725 86592251 ID=MsaG005915
Chr1 mRNA 86592251 86597725 86592251 ID=MsaT005915.1;Parent=MsaG005915
Chr1 exon 86592251 86592595 86592251 ID=MsaT005915.1.exon1;Parent=MsaT005915.1
Chr1 CDS 86592251 86592595 86592251 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86592748 86592861 86592748 ID=MsaT005915.1.exon2;Parent=MsaT005915.1
Chr1 CDS 86592748 86592861 86592748 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86592957 86593030 86592957 ID=MsaT005915.1.exon3;Parent=MsaT005915.1
Chr1 CDS 86592957 86593030 86592957 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86593357 86593426 86593357 ID=MsaT005915.1.exon4;Parent=MsaT005915.1
Chr1 CDS 86593357 86593426 86593357 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86593505 86593560 86593505 ID=MsaT005915.1.exon5;Parent=MsaT005915.1
Chr1 CDS 86593505 86593560 86593505 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86594300 86594371 86594300 ID=MsaT005915.1.exon6;Parent=MsaT005915.1
Chr1 CDS 86594300 86594371 86594300 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86594529 86594623 86594529 ID=MsaT005915.1.exon7;Parent=MsaT005915.1
Chr1 CDS 86594529 86594623 86594529 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86594722 86594788 86594722 ID=MsaT005915.1.exon8;Parent=MsaT005915.1
Chr1 CDS 86594722 86594788 86594722 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86594918 86595047 86594918 ID=MsaT005915.1.exon9;Parent=MsaT005915.1
Chr1 CDS 86594918 86595047 86594918 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86595139 86595357 86595139 ID=MsaT005915.1.exon10;Parent=MsaT005915.1
Chr1 CDS 86595139 86595357 86595139 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86595471 86595601 86595471 ID=MsaT005915.1.exon11;Parent=MsaT005915.1
Chr1 CDS 86595471 86595601 86595471 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86595730 86595904 86595730 ID=MsaT005915.1.exon12;Parent=MsaT005915.1
Chr1 CDS 86595730 86595904 86595730 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86595992 86596082 86595992 ID=MsaT005915.1.exon13;Parent=MsaT005915.1
Chr1 CDS 86595992 86596082 86595992 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86597161 86597318 86597161 ID=MsaT005915.1.exon14;Parent=MsaT005915.1
Chr1 CDS 86597161 86597318 86597161 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86597394 86597582 86597394 ID=MsaT005915.1.exon15;Parent=MsaT005915.1
Chr1 CDS 86597394 86597582 86597394 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Chr1 exon 86597666 86597725 86597666 ID=MsaT005915.1.exon16;Parent=MsaT005915.1
Chr1 CDS 86597666 86597725 86597666 ID=cds.MsaT005915.1;Parent=MsaT005915.1
Gene Sequence

>MsaT005915.1

ATGCCTCAGCTTCGTAGTGGTGTTCGACGTCCTCGTCCTGCTGCTCCTTCTCCTCCTCCGCCACCCAAGCCGGCCACCAGAGGTAGACCCAGAACTAGATTAGCCGCCGCCAAGAACATCAACAAATCATCCCAGGAGGAGGACCCACCGCCGTTGTTATTACAACCTGATACTTTCGTTGTTGAACCTCCCAAAGAGAACATAGTAATTGAACAAGAAGAAGAAGAAGACGAAGAAGCTGTTGGTGTGATGGGTGATGAAAGAGTTGGTCTCAGTGCTAATAAAGATAAGGCTGTTGCTGCTGTTCCCGAGGAAGATGCCAACTCTCCACCTCTTCCAGAAAGGGTTCAAGTAGGAGGATCGCCTCTATACAAGGTAGAAAGGAAACTTGGTAAAGGTGGATTTGGCCAGGTTTTTGTTGGCCGTCGTGAGCGTGCAAATGCACCAGGAGCCGTCGAGGTTGCTCTGAAATTTGAACATAGAAACAGTAAAGGTTGCAACTATGGCCCTCCTTATGAGTGGCAAGTATACAACACCCTTGGTGGTAGTCATGGAATACCTAAAGTACACTATAAAGGAAGACAAGGAGAATATTATGTGATGGTTATGGACATTCTTGGTCCAAGTTTGTGGGATGTATGGAATACCTCAGGCCAGTCGATGTCTCCTGAAATGGTCTCATGTATAGCTGTCGAGTCATTATCAATACTTGAAAAGTTGCATGCAAAAGGTTATGTACATGGAGATGTAAAACCAGAGAACTTTTTACTAGGCCAGCCCAACACAGCACAGGAGAAGAAATTGTTTCTTGTTGACCTTGGTTTAGCAACAAAGTGGAAAGATTCGTCAAGTGGAAAGCACACCGAATACGATCAACGCCCCGACATGTTTAGAGGGACTGTTCGATATGCTAGCGTTCATGCTCATTTGGGAAGAACTGCCAGTAGAAGGGATGATCTTGAATCACTTGCATATACACTAATCTTCCTTCATAGGGGTCGATTGCCATGGCAAGGTTTTCAGGGTGATGGCAAATCCTTCCTCGTTTGCAAAAAGAAAATGGGAACATCTGCTGAGATGCTGTGCTGCTTTTGCCCCGCTCCTTTTAAACAATTTCTCGAGACTGTGGTGAACATGAAGTTTGATGAAGAACCTAACTATTCCAAGCTAATATCCTTGTTTGATGGTATGCTCGGACCTAATCCTGCATTGCGGCCAATTAATACTGAAGGTGCTCAAAAGGTTGGGCAAAAGCGGGGTAGATTGAATGTTGAGGCAGATGATGATTCACAGCCCAAAAAGAAGGTTCGTTCAGGGATTCCTGCTTCGCAATGGATTTCAGTGTACAATGCTAGACAACCAATGAAACAAAGGTACCATTACAATGTGGCTGATGGAAGATTAGCACAGCATATTGAGAGAGGGATTGCAGACGACCTTCTTATTAGCTGTGTATCATGTTGTTCCAATCTCTGGGCACTTATAATGGATGCTGGAACTAATTATTCGGCTCAAGTTTACAAATTATCACCATTATTTTTACACAAGGAATGGATCTTGGAACAATGGGATAAGAATTTCTACATTACTTCTATTGCAGGGGCCAGTAATGGAAGCTCTGTTGTGGTGATGTCAAAAGGTACAAGGTATACTCAACAATCATACAAAGTAAGCGAATCTTTCCCCTACAAATGGATAAACAAGAAGTGGAAAGAAGGTTTTCATGTCACCTCAATGGGTACTGCTGGAAATCGCTGGGGTATTGTTATGTCTCGCAACGCTGGATTCAGTGATCAGGTGGTGGAACTTGATTTTCTCTATCCAAGTGAAGGCATCCATAAACGTTGGGATAATGGTTATAGGATTACAGCAACTGCAGCTACATTGGATCAATCTGCGCTTATATTAAGCATCCCGAGGAACAGACCTGGTGATGAAACTCAGGAAACTCTGCGTACATCTCAATTTCCCAGCGCTCATGTTAAGGAAAAATGGTCAAAAAACCTTTATCTTGCCCATTTGTGCTATGGGCGCACAGTATGCTGA
Protein sequence

>MsaT005915.1

MPQLRSGVRRPRPAAPSPPPPPKPATRGRPRTRLAAAKNINKSSQEEDPPPLLLQPDTFVVEPPKENIVIEQEEEEDEEAVGVMGDERVGLSANKDKAVAAVPEEDANSPPLPERVQVGGSPLYKVERKLGKGGFGQVFVGRRERANAPGAVEVALKFEHRNSKGCNYGPPYEWQVYNTLGGSHGIPKVHYKGRQGEYYVMVMDILGPSLWDVWNTSGQSMSPEMVSCIAVESLSILEKLHAKGYVHGDVKPENFLLGQPNTAQEKKLFLVDLGLATKWKDSSSGKHTEYDQRPDMFRGTVRYASVHAHLGRTASRRDDLESLAYTLIFLHRGRLPWQGFQGDGKSFLVCKKKMGTSAEMLCCFCPAPFKQFLETVVNMKFDEEPNYSKLISLFDGMLGPNPALRPINTEGAQKVGQKRGRLNVEADDDSQPKKKVRSGIPASQWISVYNARQPMKQRYHYNVADGRLAQHIERGIADDLLISCVSCCSNLWALIMDAGTNYSAQVYKLSPLFLHKEWILEQWDKNFYITSIAGASNGSSVVVMSKGTRYTQQSYKVSESFPYKWINKKWKEGFHVTSMGTAGNRWGIVMSRNAGFSDQVVELDFLYPSEGIHKRWDNGYRITATAATLDQSALILSIPRNRPGDETQETLRTSQFPSAHVKEKWSKNLYLAHLCYGRTVC*