AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG005977

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005977 MtrunA17_Chr2g0276901 88.377 499 56 2 239 736 21 518 0.0 860
MsaG005977 MtrunA17_Chr2g0276901 74.483 290 68 3 553 839 254 540 6.17e-119 372
MsaG005977 MtrunA17_Chr2g0276891 81.967 244 37 2 1 238 1 243 7.62e-103 320
MsaG005977 MtrunA17_Chr2g0320801 40.120 167 95 2 179 343 9 172 5.38e-34 133
MsaG005977 MtrunA17_Chr1g0162821 40.120 167 95 2 179 343 9 172 2.05e-33 130
MsaG005977 MtrunA17_Chr7g0265751 34.872 195 95 4 172 343 1 186 3.12e-32 128
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG005977 AT1G10320.2 57.907 430 140 7 4 404 34 451 1.93e-167 506
MsaG005977 AT1G10320.1 57.110 436 143 7 4 404 34 460 8.96e-167 504
MsaG005977 AT1G27650.1 40.120 167 95 2 179 343 9 172 2.24e-33 131
MsaG005977 AT5G42820.1 40.120 167 95 2 179 343 9 172 3.96e-33 130
MsaG005977 AT5G42820.2 40.120 167 95 2 179 343 9 172 3.96e-33 130
MsaG005977 AT1G27650.2 33.333 126 79 2 221 344 1 123 8.43e-16 78.6

Find 232 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGTTCTTCTTCTTGTTCT+AGG 0.136086 2:-221981 None:intergenic
AAGGAGATGGTTGCTTTATT+TGG 0.208040 2:+225445 MsaT005977.1:CDS
ATCCTTTCCTTTGGCTACTA+TGG 0.214301 2:-227101 None:intergenic
TCTCCTTTACCCAAAATTTC+GGG 0.233836 2:-225429 None:intergenic
ATTGAAATACCAGAAGAAAT+TGG 0.246434 2:+227587 MsaT005977.1:CDS
ATCTCCTTTACCCAAAATTT+CGG 0.247747 2:-225430 None:intergenic
ATAGTCCTCCGGAGATTATT+TGG 0.251192 2:+222082 MsaT005977.1:CDS
CTTGCTTATAACTCAGTTAA+TGG 0.266838 2:+224235 MsaT005977.1:CDS
ACCAGTTCAGTTCCGAAATT+AGG 0.269563 2:-222363 None:intergenic
AACCTCTTTCTCTCCTCTTC+AGG 0.274144 2:-221859 None:intergenic
ATCCTTTCCTTCGGCTACTA+TGG 0.283540 2:-226618 None:intergenic
CTAGGAAAAGCTCATTTAAT+AGG 0.296144 2:+226407 MsaT005977.1:CDS
TGGTGGAATCCAATTTCTTC+TGG 0.297692 2:-227596 None:intergenic
TTCATGGTCTCTATCTTTGC+CGG 0.320283 2:-227172 None:intergenic
GTTAATGGTCGCTACTTTGC+TGG 0.323946 2:+224250 MsaT005977.1:CDS
ATTCTTTCATTTGGCTACTA+AGG 0.330531 2:-227344 None:intergenic
TGGAGGAGAAGAAGGGGATT+GGG 0.331261 2:-222153 None:intergenic
GATGTTTCAATCCTTTCCTT+TGG 0.335919 2:-227110 None:intergenic
TCACAATTTCACCAACCTTC+AGG 0.339275 2:-224020 None:intergenic
ACCTGTCAGAATCTGTTTCT+AGG 0.342568 2:-225537 None:intergenic
TAGATAGGGTTACTATAAAC+AGG 0.344706 2:+226473 MsaT005977.1:CDS
ACTCAAGAAGATCAAGATCT+AGG 0.351478 2:+226188 MsaT005977.1:CDS
GCTCAACAACTACCTAATTT+CGG 0.351598 2:+222351 MsaT005977.1:CDS
GATGTCTAGATTCTTTCATT+TGG 0.352316 2:-227353 None:intergenic
TTTGTATGTCTATCCTCATC+TGG 0.352950 2:-227072 None:intergenic
AAGAGTATTCTGATGTAGAT+AGG 0.365009 2:+226458 MsaT005977.1:CDS
ATCTGTTTCTAGGATATTGC+TGG 0.365763 2:-225527 None:intergenic
AGGGAGATGGGTCAATTAAA+TGG 0.367639 2:+226208 MsaT005977.1:CDS
CCACATCAGAATACTCTTCA+TGG 0.370587 2:-226705 None:intergenic
CCACATCAGAATACTCTTCA+TGG 0.370969 2:-227188 None:intergenic
AAGGGTTAGAAGAGGTAGAT+AGG 0.372781 2:+226365 MsaT005977.1:CDS
GAGAAGGGTGCAGGACTTAT+CGG 0.373402 2:-222543 None:intergenic
GGAACTCACAAAAGTGGATC+TGG 0.378231 2:+226527 MsaT005977.1:CDS
ATGAGGATAGACATAGAAAA+AGG 0.379408 2:+226836 MsaT005977.1:CDS
GAGGTTGAGAGGGAGATTCC+GGG 0.379436 2:-222317 None:intergenic
AGAGGTTGAGAGGGAGATTC+CGG 0.382974 2:-222318 None:intergenic
AACGAAAATGATAAAGTTGA+TGG 0.383358 2:+227510 MsaT005977.1:CDS
TTGGTATTCCATTCCTCATC+AGG 0.390772 2:-226280 None:intergenic
ACCTAATTTCGGAACTGAAC+TGG 0.393701 2:+222362 MsaT005977.1:CDS
CATAGTCTCCGCCAGGATTA+CGG 0.395908 2:-225381 None:intergenic
ATGAGGATAGACATACAAAA+AGG 0.397308 2:+227076 MsaT005977.1:CDS
ATACCTGGTGCTGGTGGTTG+TGG 0.397792 2:-222173 None:intergenic
TGTTCTTGTCTGTCTTCGCT+TGG 0.399558 2:-227642 None:intergenic
TTTATGATTGTATTTAAAGT+TGG 0.404123 2:-226299 None:intergenic
GTGGAGGAGAAGAAGGGGAT+TGG 0.407602 2:-222154 None:intergenic
TCGCTACTTTGCTGGGAAAC+AGG 0.416456 2:+224258 MsaT005977.1:CDS
GATTGCTGAGAAGGAACGTT+TGG 0.417206 2:+221813 MsaT005977.1:CDS
GTTCTTGTCTGTCTTCGCTT+GGG 0.423470 2:-227641 None:intergenic
ATCCTTTCCTTTGGCTACCA+TGG 0.425229 2:-226861 None:intergenic
GATGTCTTGATCCTTTCCTT+TGG 0.427167 2:-226870 None:intergenic
AGAAGGGTGCAGGACTTATC+GGG 0.427879 2:-222542 None:intergenic
AAAACTGCAGCATGTCGTTT+CGG 0.428061 2:+222491 MsaT005977.1:CDS
AGATGGAAGGTTGCCATTTG+TGG 0.428325 2:+225089 MsaT005977.1:CDS
CTGATCCAGCCTCATCAATC+TGG 0.433829 2:-227296 None:intergenic
CTACGTGCAGTATAAGTTGT+TGG 0.433986 2:+224201 MsaT005977.1:CDS
GGTGGTTGTGGAGGAGAAGA+AGG 0.435899 2:-222161 None:intergenic
ATGAGTATTCTGACGTGGAT+TGG 0.437098 2:+226950 MsaT005977.1:CDS
TGAGTATTCTGACGTGGATT+GGG 0.439025 2:+226951 MsaT005977.1:CDS
TGTGGCGAGTATATGAAATC+AGG 0.441103 2:+225107 MsaT005977.1:CDS
AAAAGTGGAGCTGGGGTAGT+AGG 0.443968 2:+227028 MsaT005977.1:CDS
TCGTTCTAGTAGAAGAAAAC+AGG 0.444811 2:+226234 MsaT005977.1:CDS
GGAGAGAAAGAGGTTACTCT+TGG 0.451835 2:+221867 MsaT005977.1:CDS
ATTCACTGTTTCCGTAATCC+TGG 0.453715 2:+225370 MsaT005977.1:CDS
ATTGAAGAAATTGAAGAGAA+AGG 0.454676 2:+221777 MsaT005977.1:CDS
CTTCTGATTCTGATTCTGAC+AGG 0.458819 2:+226341 MsaT005977.1:CDS
CAATGGTCCTGGACTTGCTT+GGG 0.459683 2:+222580 MsaT005977.1:CDS
GAAAAGCAGCATGGCAGTAA+AGG 0.462211 2:+226754 MsaT005977.1:CDS
AGTGGATCTGGGATAGTAGT+AGG 0.462625 2:+226539 MsaT005977.1:CDS
CTGGTTCAGCCTCATGAATC+CGG 0.463492 2:-227053 None:intergenic
TCGAAGAGAGGGAGAAAGCT+TGG 0.466922 2:+221932 MsaT005977.1:CDS
GTTTCGGTGACCGATGTAGC+AGG 0.471525 2:+222507 MsaT005977.1:CDS
AGAAATGGACCCTGCTACAT+CGG 0.473301 2:-222517 None:intergenic
GTAACTGATAAATCTGAATC+CGG 0.474468 2:+227153 MsaT005977.1:CDS
TGACAAAGATAGAGGAGAGA+TGG 0.476773 2:+227410 MsaT005977.1:CDS
GGAGGAGAAGAAGGGGATTG+GGG 0.478173 2:-222152 None:intergenic
ATAAGAGGAATGAGGATAGT+AGG 0.480293 2:+227271 MsaT005977.1:CDS
AAGGATCAAGACATCGGAGC+AGG 0.483365 2:+226878 MsaT005977.1:CDS
GAAAAGCAGCATGATAGAAA+AGG 0.484957 2:+226502 MsaT005977.1:CDS
AAGGAAAGAACTCACATAAG+AGG 0.486874 2:+227256 MsaT005977.1:CDS
GGAACTCACAAAAGTGGAGC+TGG 0.487506 2:+227019 MsaT005977.1:CDS
TAAAATTGCAAGCTGTTCCA+TGG 0.487680 2:-225348 None:intergenic
CTGATCCAGCCTCATAAATC+CGG 0.487762 2:-226813 None:intergenic
CTGATAATTGTGAGATGTCA+AGG 0.489784 2:+225473 MsaT005977.1:CDS
ACGAAAATGATAAAGTTGAT+GGG 0.490047 2:+227511 MsaT005977.1:CDS
GATGTCTCGATCCTTTCCTT+CGG 0.490801 2:-226627 None:intergenic
AATTTGTTAATTTAACAAGA+TGG 0.492703 2:+225072 MsaT005977.1:CDS
AAGGAAAGAGATTGCTGAGA+AGG 0.497171 2:+221804 MsaT005977.1:CDS
ATCCTGGCGGAGACTATGAA+TGG 0.498169 2:+225386 MsaT005977.1:CDS
GTGGTTGTGGAGGAGAAGAA+GGG 0.501963 2:-222160 None:intergenic
TTAATGGTCGCTACTTTGCT+GGG 0.503154 2:+224251 MsaT005977.1:CDS
CTCAAGAAGATCAAGATCTA+GGG 0.503633 2:+226189 MsaT005977.1:CDS
GACAAAGATAGAGGAGAGAT+GGG 0.504372 2:+227411 MsaT005977.1:CDS
TGCTGAGAAGGAACGTTTGG+AGG 0.505737 2:+221816 MsaT005977.1:CDS
TGGTGAAATTGTGAACTTCA+AGG 0.506729 2:+224029 MsaT005977.1:CDS
AGGACTTATCGGGGTAGAAA+TGG 0.507755 2:-222532 None:intergenic
AAGGATTGAAACATCAGAGC+AGG 0.510912 2:+227118 MsaT005977.1:CDS
AAAGGAAGGAACTCGCAAAA+GGG 0.512313 2:+226772 MsaT005977.1:CDS
AAGAGTATTCTGATGTGGAT+TGG 0.513319 2:+226710 MsaT005977.1:CDS
AAGAGTATTCTGATGTGGAT+TGG 0.513319 2:+227193 MsaT005977.1:CDS
TAAAGGAAGGAACTCGCAAA+AGG 0.514540 2:+226771 MsaT005977.1:CDS
AGAGTATTCTGATGTGGATT+GGG 0.515015 2:+226711 MsaT005977.1:CDS
AGAGTATTCTGATGTGGATT+GGG 0.515015 2:+227194 MsaT005977.1:CDS
CTTGCAAGACAACTCTCCAT+TGG 0.516208 2:-227616 None:intergenic
TCTGAATCTGACAAAGATAG+AGG 0.517598 2:+226682 MsaT005977.1:CDS
AAGATCAAGATCTAGGGAGA+TGG 0.519290 2:+226195 MsaT005977.1:CDS
GATGAATCTGACAAAGATAG+AGG 0.520588 2:+227402 MsaT005977.1:CDS
TTTCATATACTCGCCACAAA+TGG 0.520774 2:-225102 None:intergenic
AATTGAAGAGAAAGGAGAGA+AGG 0.521748 2:+221785 MsaT005977.1:CDS
AACATTGTGCAGAGGTTGAG+AGG 0.522448 2:-222328 None:intergenic
AAGAAATTGGATTCCACCAA+TGG 0.523668 2:+227600 MsaT005977.1:CDS
ATCCTGAAGAGGAGAGAAAG+AGG 0.523772 2:+221857 MsaT005977.1:CDS
TGTGAGATGTCAAGGGTCCA+TGG 0.526249 2:+225481 MsaT005977.1:CDS
CACACAGAGTTCCTGAAGGT+TGG 0.527492 2:+224009 MsaT005977.1:CDS
TCCTAGAAACAGATTCTGAC+AGG 0.529003 2:+225536 MsaT005977.1:CDS
AGGAGCTGGATTCATGAGGC+TGG 0.530130 2:+226559 MsaT005977.1:CDS
TCTTGTTCCCAAGCAAGTCC+AGG 0.531043 2:-222587 None:intergenic
TGTTAATTTAACAAGATGGA+AGG 0.531596 2:+225076 MsaT005977.1:CDS
GCCCATTCATAGTCTCCGCC+AGG 0.533903 2:-225388 None:intergenic
TGGATTGGGATACTATGAGT+AGG 0.534110 2:+226725 MsaT005977.1:CDS
CGAAAATGATAAAGTTGATG+GGG 0.535498 2:+227512 MsaT005977.1:CDS
TTTCCTAGCCAAATAATCTC+CGG 0.539571 2:-222090 None:intergenic
ACAATCATAAAAGAAAATAC+AGG 0.539712 2:+226311 MsaT005977.1:CDS
GATTCTGATTCTGACAGGGA+AGG 0.541505 2:+226346 MsaT005977.1:CDS
AAGGAAGGAACTCACAAAAG+TGG 0.543036 2:+226521 MsaT005977.1:CDS
TCAGCGTGCTAAAATTTCCA+TGG 0.545766 2:-225498 None:intergenic
AGAGTATTCTGATGTAGATA+GGG 0.548177 2:+226459 MsaT005977.1:CDS
AAGAATCTAGACATCAAAGC+GGG 0.548850 2:+227361 MsaT005977.1:CDS
AGATCAAGATCTAGGGAGAT+GGG 0.548935 2:+226196 MsaT005977.1:CDS
AGGTACCAGATTGATGAGGC+TGG 0.549430 2:+227291 MsaT005977.1:CDS
GCAAGACAACTCTCCATTGG+TGG 0.550205 2:-227613 None:intergenic
ATAACATATATACCTGGTGC+TGG 0.550671 2:-222182 None:intergenic
AGATACCGGATTTATGAGGC+TGG 0.552003 2:+226808 MsaT005977.1:CDS
GATGAGGATAGAGATGGAGA+TGG 0.553244 2:+226586 MsaT005977.1:CDS
GAGTCTGAATCTGACAAAGA+TGG 0.554962 2:+226922 MsaT005977.1:CDS
CACCATAGTAGCCGAAGGAA+AGG 0.557409 2:+226616 MsaT005977.1:CDS
CTTGCTTGGGAACAAGATCA+AGG 0.558188 2:+222593 MsaT005977.1:CDS
GAACTCACAAAAGTGGATCT+GGG 0.558394 2:+226528 MsaT005977.1:CDS
TCAGAGCAGGGACAACAGTA+AGG 0.560225 2:+227131 MsaT005977.1:CDS
AAGGAAGGAACTCACAAAAG+TGG 0.560430 2:+227013 MsaT005977.1:CDS
TGATGATGATGATAGTCCTC+CGG 0.562508 2:+222071 MsaT005977.1:CDS
TCCTGGCGGAGACTATGAAT+GGG 0.563105 2:+225387 MsaT005977.1:CDS
TAGTAGATACCGGATTTATG+AGG 0.563672 2:+226804 MsaT005977.1:CDS
GGAGCTGGGGTAGTAGGTAC+CGG 0.564663 2:+227034 MsaT005977.1:CDS
CCTCCGGAGATTATTTGGCT+AGG 0.566962 2:+222087 MsaT005977.1:CDS
GAAAAGCAGCATGACAGCAA+AGG 0.568335 2:+226994 MsaT005977.1:CDS
AAAATTGCAAGCTGTTCCAT+GGG 0.569618 2:-225347 None:intergenic
CCTGGTGCTGGTGGTTGTGG+AGG 0.569821 2:-222170 None:intergenic
GAACTCACAAAAGTGGAGCT+GGG 0.570738 2:+227020 MsaT005977.1:CDS
CAAAGCGGGGACAGCTGTCA+CGG 0.570832 2:+227375 MsaT005977.1:CDS
CTCCTTTACCCAAAATTTCG+GGG 0.572507 2:-225428 None:intergenic
AAAGAACTCCTGATGAGGAA+TGG 0.572610 2:+226272 MsaT005977.1:CDS
TACATCAAATCCCTTTCCCC+CGG 0.572952 2:+222299 MsaT005977.1:CDS
TCTGGGATAGTAGTAGGAGC+TGG 0.573131 2:+226545 MsaT005977.1:CDS
CCTCCACAACCACCAGCACC+AGG 0.573575 2:+222170 MsaT005977.1:CDS
ACAATGGTCCTGGACTTGCT+TGG 0.574271 2:+222579 MsaT005977.1:CDS
GAGCACAACATGATTACTCT+AGG 0.578647 2:+227433 MsaT005977.1:CDS
ATTCTGATTCTGACAGGGAA+GGG 0.580207 2:+226347 MsaT005977.1:CDS
AGCAGCATGACAGCAAAGGA+AGG 0.581381 2:+226998 MsaT005977.1:CDS
AGCAGCATGGCAGTAAAGGA+AGG 0.581903 2:+226758 MsaT005977.1:CDS
AAGGATCGAGACATCAGAGA+AGG 0.586354 2:+226635 MsaT005977.1:CDS
GACAGACAAGAACAAAGCAA+AGG 0.587753 2:+227651 MsaT005977.1:CDS
TGGATTGGGATACTATGAGT+AGG 0.589392 2:+226965 MsaT005977.1:CDS
AGGTTGAGAGGGAGATTCCG+GGG 0.591558 2:-222316 None:intergenic
GAAAAGCAGCATGACAGCAA+AGG 0.591677 2:+227237 MsaT005977.1:CDS
GGAGATGTGGTCGAATCGAA+AGG 0.592536 2:+221741 None:intergenic
AGAGGCGGAGAGAATCGAAA+GGG 0.592841 2:+221897 MsaT005977.1:CDS
TCGACTCAACGATCCTGAAG+AGG 0.592991 2:+221846 MsaT005977.1:CDS
ACATTGTGCAGAGGTTGAGA+GGG 0.594548 2:-222327 None:intergenic
ATAGAATCGCTTTCGAAGAG+AGG 0.598605 2:+221920 MsaT005977.1:CDS
GGATTGGGATACTATGAGTA+GGG 0.598777 2:+226726 MsaT005977.1:CDS
GAACTCACATAAGAGGAATG+AGG 0.599689 2:+227263 MsaT005977.1:CDS
GGATTGGGATACTATGAGTA+GGG 0.599782 2:+226966 MsaT005977.1:CDS
TAGAATCGCTTTCGAAGAGA+GGG 0.601888 2:+221921 MsaT005977.1:CDS
GGAACAGCGATAGTAGATAC+CGG 0.602268 2:+226794 MsaT005977.1:CDS
TTCTGATTCTGATTCTGACA+GGG 0.603484 2:+226342 MsaT005977.1:CDS
GCACGTAGACATTTCCTCGC+AGG 0.603491 2:-224186 None:intergenic
CCATGAAGAGTATTCTGATG+TGG 0.603592 2:+227188 MsaT005977.1:CDS
TGGTTGTGGAGGAGAAGAAG+GGG 0.606296 2:-222159 None:intergenic
GTATATGAAATCAGGTTACA+AGG 0.607083 2:+225115 MsaT005977.1:CDS
GGAACAAGATCAAGGCCTTG+AGG 0.607210 2:+222601 MsaT005977.1:CDS
CACCATAGTAGCCAAAGGAA+AGG 0.607686 2:+227099 MsaT005977.1:CDS
CACCATGGTAGCCAAAGGAA+AGG 0.609982 2:+226859 MsaT005977.1:CDS
TGGATTGGGATACTACGAGT+AGG 0.611788 2:+227208 MsaT005977.1:CDS
CCATGAAGAGTATTCTGATG+TGG 0.613223 2:+226705 MsaT005977.1:CDS
AAGAGGCGGAGAGAATCGAA+AGG 0.615210 2:+221896 MsaT005977.1:CDS
TCTTAACAGACTTGTTCCCA+TGG 0.621930 2:+225331 MsaT005977.1:intron
GGATGTGATGAGGATAGAGA+TGG 0.622071 2:+226580 MsaT005977.1:CDS
AAAAGTATCATGAACACACT+AGG 0.623161 2:+226389 MsaT005977.1:CDS
GGATTGGGATACTACGAGTA+GGG 0.623278 2:+227209 MsaT005977.1:CDS
CACTGTTTCCGTAATCCTGG+CGG 0.623496 2:+225373 MsaT005977.1:CDS
GTTACTCTTGGAGCAGCAAG+AGG 0.624110 2:+221879 MsaT005977.1:CDS
AAAGCAAAGAACTCCTGATG+AGG 0.624280 2:+226267 MsaT005977.1:CDS
GAAAATGATAAAGTTGATGG+GGG 0.626135 2:+227513 MsaT005977.1:CDS
TGATGAGGCTGGATCAGATG+AGG 0.627514 2:+227302 MsaT005977.1:CDS
TCCTTTACCCAAAATTTCGG+GGG 0.628973 2:-225427 None:intergenic
AATGGTTCATTTCACCTGCG+AGG 0.632032 2:+224172 MsaT005977.1:CDS
TGACAGGGAAGGGTTAGAAG+AGG 0.632794 2:+226357 MsaT005977.1:CDS
AGGATCAAGACATCGGAGCA+GGG 0.633206 2:+226879 MsaT005977.1:CDS
AAGGAAGGAACTCGCAAAAG+GGG 0.633299 2:+226773 MsaT005977.1:CDS
AACTCACAAAAGTGGAGCTG+GGG 0.634655 2:+227021 MsaT005977.1:CDS
AAAGGCACCATGGTAGCCAA+AGG 0.634673 2:+226854 MsaT005977.1:CDS
AGGATCGAGACATCAGAGAA+GGG 0.634774 2:+226636 MsaT005977.1:CDS
TTATGAGGCTGGATCAGATG+AGG 0.638298 2:+226819 MsaT005977.1:CDS
TGATAATTGTGAGATGTCAA+GGG 0.643511 2:+225474 MsaT005977.1:CDS
TTCTAGTAGAAGAAAACAGG+AGG 0.644725 2:+226237 MsaT005977.1:CDS
AGCAGCATGATAGAAAAGGA+AGG 0.644760 2:+226506 MsaT005977.1:CDS
TCATGAGGCTGAACCAGATG+AGG 0.645107 2:+227059 MsaT005977.1:CDS
GGTCGAATCGAAAGGAGAAG+AGG 0.646281 2:+221749 MsaT005977.1:CDS
TTTCGGTGACCGATGTAGCA+GGG 0.649087 2:+222508 MsaT005977.1:CDS
AAAGGAAAGGATCAAGACAT+CGG 0.653130 2:+226872 MsaT005977.1:CDS
GAAGACACCATAGTAGCCGA+AGG 0.653894 2:+226611 MsaT005977.1:CDS
AAAGGCACCATAGTAGCCAA+AGG 0.654107 2:+227094 MsaT005977.1:CDS
AGGATTGAAACATCAGAGCA+GGG 0.658719 2:+227119 MsaT005977.1:CDS
TAGTAGGAGCTGGATTCATG+AGG 0.658776 2:+226555 MsaT005977.1:CDS
GAATCGAAAGGAGAAGAGGA+AGG 0.658952 2:+221753 MsaT005977.1:CDS
AAGCTCATTTAATAGGAACA+AGG 0.661186 2:+226414 MsaT005977.1:CDS
ACTCTTGGAGCAGCAAGAGG+CGG 0.662386 2:+221882 MsaT005977.1:CDS
GGTTGAGAGGGAGATTCCGG+GGG 0.671144 2:-222315 None:intergenic
TCTGAATCTGACAAAGATGG+AGG 0.674663 2:+226925 MsaT005977.1:CDS
TGTTCACACAGAGTTCCTGA+AGG 0.677382 2:+224005 MsaT005977.1:CDS
TAGTAGGTACCGGATTCATG+AGG 0.677752 2:+227044 MsaT005977.1:CDS
CCTAGCCAAATAATCTCCGG+AGG 0.678025 2:-222087 None:intergenic
TGTTGAGCAACATTGTGCAG+AGG 0.684405 2:-222336 None:intergenic
TAGTAGGTACCAGATTGATG+AGG 0.688357 2:+227287 MsaT005977.1:CDS
TCATGAGGCTGGATGTGATG+AGG 0.692514 2:+226570 MsaT005977.1:CDS
AGGGACAATGAAAAGCAGCA+TGG 0.694028 2:+226745 MsaT005977.1:CDS
GAAGGGTGCAGGACTTATCG+GGG 0.707078 2:-222541 None:intergenic
AGATAGGGTTACTATAAACA+GGG 0.714784 2:+226474 MsaT005977.1:CDS
AAAGAATCTAGACATCAAAG+CGG 0.719311 2:+227360 MsaT005977.1:CDS
AGAATCTAGACATCAAAGCG+GGG 0.720964 2:+227362 MsaT005977.1:CDS
ACATATATACCTGGTGCTGG+TGG 0.722080 2:-222179 None:intergenic
AGGACATGAGTATTCTGACG+TGG 0.730553 2:+226945 MsaT005977.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 221745 227692 221745 ID=MsaG005977
Chr2 mRNA 221745 227692 221745 ID=MsaT005977.1;Parent=MsaG005977
Chr2 exon 221745 222191 221745 ID=MsaT005977.1.exon1;Parent=MsaT005977.1
Chr2 CDS 221745 222191 221745 ID=cds.MsaT005977.1;Parent=MsaT005977.1
Chr2 exon 222282 222383 222282 ID=MsaT005977.1.exon2;Parent=MsaT005977.1
Chr2 CDS 222282 222383 222282 ID=cds.MsaT005977.1;Parent=MsaT005977.1
Chr2 exon 222464 222622 222464 ID=MsaT005977.1.exon3;Parent=MsaT005977.1
Chr2 CDS 222464 222622 222464 ID=cds.MsaT005977.1;Parent=MsaT005977.1
Chr2 exon 223958 224050 223958 ID=MsaT005977.1.exon4;Parent=MsaT005977.1
Chr2 CDS 223958 224050 223958 ID=cds.MsaT005977.1;Parent=MsaT005977.1
Chr2 exon 224163 224279 224163 ID=MsaT005977.1.exon5;Parent=MsaT005977.1
Chr2 CDS 224163 224279 224163 ID=cds.MsaT005977.1;Parent=MsaT005977.1
Chr2 exon 225062 225136 225062 ID=MsaT005977.1.exon6;Parent=MsaT005977.1
Chr2 CDS 225062 225136 225062 ID=cds.MsaT005977.1;Parent=MsaT005977.1
Chr2 exon 225340 225557 225340 ID=MsaT005977.1.exon7;Parent=MsaT005977.1
Chr2 CDS 225340 225557 225340 ID=cds.MsaT005977.1;Parent=MsaT005977.1
Chr2 exon 226183 227692 226183 ID=MsaT005977.1.exon8;Parent=MsaT005977.1
Chr2 CDS 226183 227692 226183 ID=cds.MsaT005977.1;Parent=MsaT005977.1
Gene Sequence

>MsaT005977.1

ATGTGGTCGAATCGAAAGGAGAAGAGGAAGGCATTGAAGAAATTGAAGAGAAAGGAGAGAAGGAAAGAGATTGCTGAGAAGGAACGTTTGGAGGAAGAAGCTCGACTCAACGATCCTGAAGAGGAGAGAAAGAGGTTACTCTTGGAGCAGCAAGAGGCGGAGAGAATCGAAAGGGATAGAATCGCTTTCGAAGAGAGGGAGAAAGCTTGGATTATCAAACAACAACAACAACGAGACCTAGAACAAGAAGAAGAACAAGAACTATCTCAACGAGACGATATCAAAGAAGAAGATGATAAATCAGAATTAGAAGAAGAAGAAGAAGATGATGATGATGATAGTCCTCCGGAGATTATTTGGCTAGGAAATGAAATTATCATCAAAAAGAAGAAAAAGAAAAAGCTGAACCCCAATCCCCTTCTTCTCCTCCACAACCACCAGCACCAGCATCATGATAATAGACCTACATCAAATCCCTTTCCCCCGGAATCTCCCTCTCAACCTCTGCACAATGTTGCTCAACAACTACCTAATTTCGGAACTGAACTGGATAAAGCTCACTGTCCCTTTCATCTTAAAACTGCAGCATGTCGTTTCGGTGACCGATGTAGCAGGGTCCATTTCTACCCCGATAAGTCCTGCACCCTTCTCATCAAAAACATGTACAATGGTCCTGGACTTGCTTGGGAACAAGATCAAGGCCTTGAGTATACAGATGAGGAGGTTGAACGCTGTTTTGAAGAGTTTTATGAAGATGTTCACACAGAGTTCCTGAAGGTTGGTGAAATTGTGAACTTCAAGGTGTGTAAAAATGGTTCATTTCACCTGCGAGGAAATGTCTACGTGCAGTATAAGTTGTTGGATTCTGCTCTGCTTGCTTATAACTCAGTTAATGGTCGCTACTTTGCTGGGAAACAGGTGAGCTGTAAATTTGTTAATTTAACAAGATGGAAGGTTGCCATTTGTGGCGAGTATATGAAATCAGGTTACAAGACTTGTTCCCATGGAACAGCTTGCAATTTTATTCACTGTTTCCGTAATCCTGGCGGAGACTATGAATGGGCAGATTCTGATAAACCACCCCCGAAATTTTGGGTAAAGGAGATGGTTGCTTTATTTGGTCATTCTGATAATTGTGAGATGTCAAGGGTCCATGGAAATTTTAGCACGCTGAAGCACTCCAGCAATATCCTAGAAACAGATTCTGACAGGTATCACTCAAGAAGATCAAGATCTAGGGAGATGGGTCAATTAAATGGTAGTCGTTCTAGTAGAAGAAAACAGGAGGATGAAAGAAAGCAAAGAACTCCTGATGAGGAATGGAATACCAACTTTAAATACAATCATAAAAGAAAATACAGGAACAAAACTTCTGATTCTGATTCTGACAGGGAAGGGTTAGAAGAGGTAGATAGGAAAAAGTATCATGAACACACTAGGAAAAGCTCATTTAATAGGAACAAGGATGACAATAGCAGAAAGCATGAAGAGTATTCTGATGTAGATAGGGTTACTATAAACAGGGACAATGAAAAGCAGCATGATAGAAAAGGAAGGAACTCACAAAAGTGGATCTGGGATAGTAGTAGGAGCTGGATTCATGAGGCTGGATGTGATGAGGATAGAGATGGAGATGGAAGAAGACACCATAGTAGCCGAAGGAAAGGATCGAGACATCAGAGAAGGGACAGCAGCAATGTAACTGATGAATCTGAATCTGACAAAGATAGAGGCCATGAAGAGTATTCTGATGTGGATTGGGATACTATGAGTAGGGACAATGAAAAGCAGCATGGCAGTAAAGGAAGGAACTCGCAAAAGGGGAACAGCGATAGTAGATACCGGATTTATGAGGCTGGATCAGATGAGGATAGACATAGAAAAAGGCACCATGGTAGCCAAAGGAAAGGATCAAGACATCGGAGCAGGGACAGCAGTAATGTAGCTGACGAGTCTGAATCTGACAAAGATGGAGGACATGAGTATTCTGACGTGGATTGGGATACTATGAGTAGGGACAACGAAAAGCAGCATGACAGCAAAGGAAGGAACTCACAAAAGTGGAGCTGGGGTAGTAGGTACCGGATTCATGAGGCTGAACCAGATGAGGATAGACATACAAAAAGGCACCATAGTAGCCAAAGGAAAGGATTGAAACATCAGAGCAGGGACAACAGTAAGGTAACTGATAAATCTGAATCCGGCAAAGATAGAGACCATGAAGAGTATTCTGATGTGGATTGGGATACTACGAGTAGGGACAATGAAAAGCAGCATGACAGCAAAGGAAAGAACTCACATAAGAGGAATGAGGATAGTAGGTACCAGATTGATGAGGCTGGATCAGATGAGGATAGACATAGAAAAAAGCACCTTAGTAGCCAAATGAAAGAATCTAGACATCAAAGCGGGGACAGCTGTCACGGAATCGATGAATCTGACAAAGATAGAGGAGAGATGGGAGCACAACATGATTACTCTAGGAAAAGCTCGAGACACAAAAGATCTGATCTTCGAGACTACTGTGAAGAGTATGAAAACGAAAATGATAAAGTTGATGGGGGTTGGTCTCAGAGAGACAGTGACCGACGAGCACATCATCACAAAAAGAAGAGATTGAAATACCAGAAGAAATTGGATTCCACCAATGGAGAGTTGTCTTGCAAGAACCCAAGCGAAGACAGACAAGAACAAAGCAAAGGAAGTGACAAACACTTATAA
Protein sequence

>MsaT005977.1

MWSNRKEKRKALKKLKRKERRKEIAEKERLEEEARLNDPEEERKRLLLEQQEAERIERDRIAFEEREKAWIIKQQQQRDLEQEEEQELSQRDDIKEEDDKSELEEEEEDDDDDSPPEIIWLGNEIIIKKKKKKKLNPNPLLLLHNHQHQHHDNRPTSNPFPPESPSQPLHNVAQQLPNFGTELDKAHCPFHLKTAACRFGDRCSRVHFYPDKSCTLLIKNMYNGPGLAWEQDQGLEYTDEEVERCFEEFYEDVHTEFLKVGEIVNFKVCKNGSFHLRGNVYVQYKLLDSALLAYNSVNGRYFAGKQVSCKFVNLTRWKVAICGEYMKSGYKTCSHGTACNFIHCFRNPGGDYEWADSDKPPPKFWVKEMVALFGHSDNCEMSRVHGNFSTLKHSSNILETDSDRYHSRRSRSREMGQLNGSRSSRRKQEDERKQRTPDEEWNTNFKYNHKRKYRNKTSDSDSDREGLEEVDRKKYHEHTRKSSFNRNKDDNSRKHEEYSDVDRVTINRDNEKQHDRKGRNSQKWIWDSSRSWIHEAGCDEDRDGDGRRHHSSRRKGSRHQRRDSSNVTDESESDKDRGHEEYSDVDWDTMSRDNEKQHGSKGRNSQKGNSDSRYRIYEAGSDEDRHRKRHHGSQRKGSRHRSRDSSNVADESESDKDGGHEYSDVDWDTMSRDNEKQHDSKGRNSQKWSWGSRYRIHEAEPDEDRHTKRHHSSQRKGLKHQSRDNSKVTDKSESGKDRDHEEYSDVDWDTTSRDNEKQHDSKGKNSHKRNEDSRYQIDEAGSDEDRHRKKHLSSQMKESRHQSGDSCHGIDESDKDRGEMGAQHDYSRKSSRHKRSDLRDYCEEYENENDKVDGGWSQRDSDRRAHHHKKKRLKYQKKLDSTNGELSCKNPSEDRQEQSKGSDKHL*