AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006064


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006064 MtrunA17_Chr7g0253101 36.036 111 54 5 35 133 115 220 2.41e-11 60.1
MsaG006064 MtrunA17_Chr8g0340401 53.704 54 21 2 96 145 218 271 4.33e-11 60.1
MsaG006064 MtrunA17_Chr7g0271861 66.667 36 10 1 100 135 23 56 5.54e-11 57.0
MsaG006064 MtrunA17_Chr3g0140331 60.465 43 16 1 104 145 256 298 6.93e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006064 AT3G20750.1 61.538 39 15 0 101 139 159 197 7.01e-13 63.9
MsaG006064 AT5G25830.1 42.466 73 34 2 81 145 192 264 1.72e-11 61.6
MsaG006064 AT4G32890.1 73.333 30 8 0 104 133 200 229 2.87e-11 60.8

Find 45 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTGCTGCTGTCTCAATTT+TGG 0.174192 2:+1176973 MsaT006064.1:CDS
CTTCTTCTTCAACTTCCTAA+TGG 0.279260 2:+1177986 MsaT006064.1:CDS
ACGTCCTCCTCCTCAAAATA+TGG 0.327173 2:-1175995 None:intergenic
TATGTGGCGCTCTGGACCTA+TGG 0.393114 2:+1177159 MsaT006064.1:CDS
GGTTGGACATTGTTTGCAAT+TGG 0.397971 2:-1176935 None:intergenic
GCAATTGGAGCAGCATCAAT+TGG 0.414015 2:-1176956 None:intergenic
CGAGATGTCCCTGCTGCCTC+AGG 0.421876 2:+1177928 MsaT006064.1:CDS
GGATCTCCTCCCATGATTCC+AGG 0.423283 2:-1177964 None:intergenic
TGTCCCACCACCTGGAATCA+TGG 0.424311 2:+1177954 MsaT006064.1:CDS
AAAATTGAGACAGCAGCAAT+TGG 0.438976 2:-1176971 None:intergenic
AACACTCCTATGTGGCGCTC+TGG 0.449276 2:+1177151 MsaT006064.1:CDS
TCAAGAAATGCCATTGGGCG+TGG 0.452536 2:+1177045 MsaT006064.1:CDS
TCAGGTTCTGTCCCACCACC+TGG 0.454678 2:+1177946 MsaT006064.1:CDS
CCTCCCATGATTCCAGGTGG+TGG 0.456756 2:-1177958 None:intergenic
CATTTACTACAAGAAACATA+TGG 0.481434 2:+1177900 MsaT006064.1:CDS
GGGACAGAACCTGAGGCAGC+AGG 0.482176 2:-1177937 None:intergenic
ATGTGGCGCTCTGGACCTAT+GGG 0.485189 2:+1177160 MsaT006064.1:CDS
GAATTCAGACACTGAAAGTC+AGG 0.494441 2:+1176018 MsaT006064.1:CDS
TTTCTTGATCGGCACGGACA+TGG 0.499473 2:-1177030 None:intergenic
GTCCCACCACCTGGAATCAT+GGG 0.504732 2:+1177955 MsaT006064.1:CDS
TTGGAGCAGCATCAATTGGT+TGG 0.505822 2:-1176952 None:intergenic
CAGAAGGTGGTCGGATCAGA+TGG 0.507419 2:-1177103 None:intergenic
AAATTTACCTTTGGACCCAT+AGG 0.510533 2:-1177175 None:intergenic
CTCCCATGATTCCAGGTGGT+GGG 0.519420 2:-1177957 None:intergenic
GCCCAATGGCATTTCTTGAT+CGG 0.524113 2:-1177041 None:intergenic
GCCGATCAAGAAATGCCATT+GGG 0.532200 2:+1177040 MsaT006064.1:CDS
CATTAGGAAGTTGAAGAAGA+AGG 0.548858 2:-1177985 None:intergenic
TGCCGATCAAGAAATGCCAT+TGG 0.553175 2:+1177039 MsaT006064.1:CDS
CGACGTCGTCCCACGCCCAA+TGG 0.553398 2:-1177055 None:intergenic
CTCTGGACCTATGGGTCCAA+AGG 0.554114 2:+1177168 MsaT006064.1:CDS
GCAATGAAAATTTGTGCAGA+AGG 0.580559 2:-1177119 None:intergenic
CAAGAAATGCCATTGGGCGT+GGG 0.597462 2:+1177046 MsaT006064.1:CDS
GGACAGAACCTGAGGCAGCA+GGG 0.617193 2:-1177936 None:intergenic
AAAGAATCAGTACAATACAT+TGG 0.618095 2:-1177007 None:intergenic
ATGGCATTTCTTGATCGGCA+CGG 0.620469 2:-1177036 None:intergenic
AAATTTGTGCAGAAGGTGGT+CGG 0.623430 2:-1177112 None:intergenic
ATGAAAATTTGTGCAGAAGG+TGG 0.644289 2:-1177116 None:intergenic
AGACAAGAAACACTCCTATG+TGG 0.647405 2:+1177143 MsaT006064.1:CDS
ATAGGTCCAGAGCGCCACAT+AGG 0.654562 2:-1177157 None:intergenic
CAGACTTTGTGCAATGCGTG+TGG 0.657380 2:+1177877 MsaT006064.1:intron
AGGTGGTGGGACAGAACCTG+AGG 0.657762 2:-1177944 None:intergenic
CAGATGGACGAGCTCGTCCG+CGG 0.666213 2:-1177087 None:intergenic
CCACCACCTGGAATCATGGG+AGG 0.682520 2:+1177958 MsaT006064.1:CDS
TCTCCTCCCATGATTCCAGG+TGG 0.693154 2:-1177961 None:intergenic
CGACGTCGTTACATCAGCCG+CGG 0.703348 2:+1177070 MsaT006064.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 1175974 1178005 1175974 ID=MsaG006064
Chr2 mRNA 1175974 1178005 1175974 ID=MsaT006064.1;Parent=MsaG006064
Chr2 exon 1175974 1176081 1175974 ID=MsaT006064.1.exon1;Parent=MsaT006064.1
Chr2 CDS 1175974 1176081 1175974 ID=cds.MsaT006064.1;Parent=MsaT006064.1
Chr2 exon 1176932 1177189 1176932 ID=MsaT006064.1.exon2;Parent=MsaT006064.1
Chr2 CDS 1176932 1177189 1176932 ID=cds.MsaT006064.1;Parent=MsaT006064.1
Chr2 exon 1177880 1178005 1177880 ID=MsaT006064.1.exon3;Parent=MsaT006064.1
Chr2 CDS 1177880 1178005 1177880 ID=cds.MsaT006064.1;Parent=MsaT006064.1
Gene Sequence

>MsaT006064.1

ATGGACTTTGACTTAAACATGCCATATTTTGAGGAGGAGGACGTGAATTCAGACACTGAAAGTCAGGTTTCATCACACATTCAGAGCAATACTGAAAATATCCCTAAAGATCCAATTGCAAACAATGTCCAACCAATTGATGCTGCTCCAATTGCTGCTGTCTCAATTTTGGTTGATGCTCAACCAATGTATTGTACTGATTCTTTCCATGTCCGTGCCGATCAAGAAATGCCATTGGGCGTGGGACGACGTCGTTACATCAGCCGCGGACGAGCTCGTCCATCTGATCCGACCACCTTCTGCACAAATTTTCATTGCAAGACAAGAAACACTCCTATGTGGCGCTCTGGACCTATGGGTCCAAAGACTTTGTGCAATGCGTGTGGCATTTACTACAAGAAACATATGGTTCGTCGAGATGTCCCTGCTGCCTCAGGTTCTGTCCCACCACCTGGAATCATGGGAGGAGATCCTTCTTCTTCAACTTCCTAA

Protein sequence

>MsaT006064.1

MDFDLNMPYFEEEDVNSDTESQVSSHIQSNTENIPKDPIANNVQPIDAAPIAAVSILVDAQPMYCTDSFHVRADQEMPLGVGRRRYISRGRARPSDPTTFCTNFHCKTRNTPMWRSGPMGPKTLCNACGIYYKKHMVRRDVPAASGSVPPPGIMGGDPSSSTS*