AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006091

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006091 MtrunA17_Chr2g0277361 93.952 463 8 9 1 462 1 444 0.0 844
MsaG006091 MtrunA17_Chr5g0416581 92.045 88 6 1 129 215 61 148 1.95e-49 174
MsaG006091 MtrunA17_Chr7g0224581 52.632 152 62 3 106 253 21 166 3.57e-38 141
MsaG006091 MtrunA17_Chr1g0211941 80.488 82 13 1 125 206 27 105 4.89e-38 140
MsaG006091 MtrunA17_Chr1g0204001 56.303 119 48 1 104 222 38 152 1.09e-36 138
MsaG006091 MtrunA17_Chr1g0164881 75.000 84 18 1 119 202 20 100 1.05e-35 133
MsaG006091 MtrunA17_Chr8g0350941 78.947 76 16 0 131 206 77 152 2.07e-35 135
MsaG006091 MtrunA17_Chr1g0180911 80.282 71 14 0 126 196 41 111 9.60e-35 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006091 AT1G58100.1 46.824 425 129 17 75 475 13 364 9.97e-81 257
MsaG006091 AT1G35560.1 43.027 337 94 10 131 461 52 296 2.24e-59 200
MsaG006091 AT1G58100.2 41.647 425 127 19 75 475 13 340 4.51e-57 195
MsaG006091 AT1G72010.1 40.604 298 104 10 123 397 52 299 4.65e-48 171
MsaG006091 AT1G69690.1 58.599 157 38 3 129 273 46 187 1.62e-41 152
MsaG006091 AT3G47620.1 82.353 85 13 1 121 203 102 186 2.07e-40 152
MsaG006091 AT5G23280.1 77.108 83 16 1 124 206 32 111 5.58e-37 137
MsaG006091 AT2G45680.1 57.600 125 41 3 88 211 39 152 4.96e-36 137
MsaG006091 AT5G08330.1 68.750 96 27 1 109 204 9 101 1.15e-35 133
MsaG006091 AT3G27010.2 51.969 127 45 2 69 195 29 139 2.00e-34 132
MsaG006091 AT3G27010.1 51.969 127 45 2 69 195 29 139 2.00e-34 132
MsaG006091 AT5G51910.1 78.205 78 16 1 130 206 52 129 8.53e-34 130
MsaG006091 AT5G51910.2 78.205 78 16 1 130 206 52 129 8.53e-34 130
MsaG006091 AT2G37000.1 80.000 60 12 0 134 193 43 102 3.53e-27 108
MsaG006091 AT5G41030.1 51.546 97 35 1 131 227 65 149 1.59e-26 108
MsaG006091 AT3G45150.1 61.905 63 24 0 131 193 14 76 1.16e-21 92.4

Find 150 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGCCATCACCTTTCATTA+TGG 0.235639 2:+1479088 MsaT006091.1:CDS
ATGATTCTTGTTGGTGGTGG+TGG 0.240254 2:-1479016 None:intergenic
AGGTTCAATGTTCCAGCTTC+TGG 0.242129 2:+1480208 MsaT006091.1:CDS
GTTTGAAGAAGGTTGAATAT+TGG 0.260266 2:-1479120 None:intergenic
CTTGTTGGTGGTGGTGGGGA+TGG 0.267306 2:-1479010 None:intergenic
TTGAAGAAAATGGTTGAATT+TGG 0.273936 2:-1479283 None:intergenic
TCACCAACAACAGGATGATA+TGG 0.275830 2:+1479892 MsaT006091.1:CDS
TGTTGGTGGTGGTGGGGATG+GGG 0.283293 2:-1479008 None:intergenic
TTGTTGGTGGTGGTGGGGAT+GGG 0.292871 2:-1479009 None:intergenic
TTCCAGTTGACCAGAGAATT+AGG 0.308053 2:+1479425 MsaT006091.1:CDS
GATAGTCATTGTGAGGTATT+AGG 0.316778 2:-1480484 None:intergenic
GACGGTGGTGGAGGAGGTGG+TGG 0.325996 2:+1479923 MsaT006091.1:CDS
AATTTGGTTGTTGTTGTTGT+TGG 0.326907 2:-1479267 None:intergenic
GACTGAACATGAGGTGTAAA+AGG 0.331793 2:-1480130 None:intergenic
CGGGGAGTGGAAGTACAATT+TGG 0.342926 2:+1480035 MsaT006091.1:CDS
TCTTCTCCACTCTCATCATT+AGG 0.347346 2:-1480457 None:intergenic
CGCCCATATCATCCTGTTGT+TGG 0.355044 2:-1479895 None:intergenic
AGGCCATCACCTTTCATTAT+GGG 0.359459 2:+1479089 MsaT006091.1:CDS
TGATTCTTGTTGGTGGTGGT+GGG 0.360015 2:-1479015 None:intergenic
GTGGGGATGGGGATGAATAT+TGG 0.363141 2:-1478997 None:intergenic
GAAGGTGACGGTGGTGGAGG+AGG 0.367290 2:+1479917 MsaT006091.1:CDS
GGCTGGGATTGTTCCGGTGC+CGG 0.367521 2:-1479516 None:intergenic
CTGTCCTTGGTGGATCGTTT+TGG 0.369093 2:-1479338 None:intergenic
TACCAACACGCTCCTCTGTT+AGG 0.370083 2:+1479668 MsaT006091.1:CDS
GTTGTTGCTGTTGCTGTTGT+TGG 0.372670 2:-1479712 None:intergenic
TGGACGTGGTGGTTTACAAT+TGG 0.382459 2:+1480258 MsaT006091.1:CDS
GTATCAACCATCACAGCATT+TGG 0.390115 2:+1480288 MsaT006091.1:CDS
GCGGTTGAAGTTGAAGAAAA+TGG 0.393103 2:-1479293 None:intergenic
GGCCTAATTCTCTGGTCAAC+TGG 0.396838 2:-1479427 None:intergenic
GTAGCCCACATGGCTGTTGC+CGG 0.398412 2:-1480010 None:intergenic
GGATTTGTGGCCTAATTCTC+TGG 0.401966 2:-1479435 None:intergenic
GGTGGTGGAGGAGGTGGTGG+TGG 0.404546 2:+1479926 MsaT006091.1:CDS
TCATCAACATTCAATGTCAT+TGG 0.411521 2:+1480414 MsaT006091.1:CDS
TTGTTTCACCATCGGATTTG+TGG 0.412133 2:-1479448 None:intergenic
GATTCTTGTTGGTGGTGGTG+GGG 0.413254 2:-1479014 None:intergenic
CCACTCTCATCATTAGGTTG+AGG 0.418843 2:-1480451 None:intergenic
GGTGACGGTGGTGGAGGAGG+TGG 0.419720 2:+1479920 MsaT006091.1:CDS
TATCAACCATCACAGCATTT+GGG 0.420346 2:+1480289 MsaT006091.1:CDS
GGACGTGGTGGTTTACAATT+GGG 0.421245 2:+1480259 MsaT006091.1:CDS
TTGTTGTTGTTGGATACATC+TGG 0.423257 2:-1479257 None:intergenic
GACGTTGAGCGTTGAGAAAT+TGG 0.424642 2:-1479537 None:intergenic
AAGAGTTGTTAACGTTGAAA+TGG 0.427409 2:-1480375 None:intergenic
GAGGGAGAAGAGTTTGAAGA+AGG 0.435751 2:-1479131 None:intergenic
GGTGGTGGTGTTGAATTTCA+AGG 0.437658 2:+1480235 MsaT006091.1:CDS
AGGATGATTCTTGTTGGTGG+TGG 0.437678 2:-1479019 None:intergenic
CAGATCTGGTGGCAATTGCC+AGG 0.437859 2:-1479223 None:intergenic
CTGATGAACGCTGATCAAAT+TGG 0.440741 2:+1479737 MsaT006091.1:CDS
GGGATGGGGATGAATATTGG+TGG 0.440791 2:-1478994 None:intergenic
GAAATTGGCTGGGATTGTTC+CGG 0.446496 2:-1479522 None:intergenic
GGCGAAGGTGACGGTGGTGG+AGG 0.448389 2:+1479914 MsaT006091.1:CDS
GGCTGTATCTGATTCCAACT+TGG 0.449042 2:+1480315 MsaT006091.1:CDS
ACAGCCATGTGGGCTACAGC+GGG 0.455998 2:+1480016 MsaT006091.1:CDS
GGAGCTTCTTCTTCTTCTGT+AGG 0.458812 2:+1479947 MsaT006091.1:CDS
ACCAACACGCTCCTCTGTTA+GGG 0.462020 2:+1479669 MsaT006091.1:CDS
GGTGATGGCCTTCCATCAAA+AGG 0.462353 2:-1479077 None:intergenic
AATGTTCCAGCTTCTGGTGG+TGG 0.463360 2:+1480214 MsaT006091.1:CDS
CTCAGTTACATTTCATGCCA+AGG 0.466286 2:+1480188 MsaT006091.1:CDS
CAAAACAAATCACCAACAAC+AGG 0.468328 2:+1479883 MsaT006091.1:CDS
AAGGTGAGGATGATTCTTGT+TGG 0.474893 2:-1479025 None:intergenic
CCATTCAATTGTTTCACCAT+CGG 0.476071 2:-1479456 None:intergenic
TTGAGCGTTGAGAAATTGGC+TGG 0.476634 2:-1479533 None:intergenic
CCAGCTTCTGGTGGTGGTGG+TGG 0.477601 2:+1480220 MsaT006091.1:CDS
ACACCTCATTGATGCTTCCC+TGG 0.479655 2:+1479205 MsaT006091.1:CDS
GCTTCTTCAACAAGCGGAGC+CGG 0.481221 2:+1479478 MsaT006091.1:CDS
TCGACCGTCAAATATGTTAC+CGG 0.485929 2:+1479991 MsaT006091.1:CDS
ATCATCGCTGCCACCGGCAC+CGG 0.486326 2:+1479503 MsaT006091.1:CDS
ATGGGCGAAGGTGACGGTGG+TGG 0.487166 2:+1479911 MsaT006091.1:CDS
ATGTGGGCTACAGCGGGGAG+TGG 0.494234 2:+1480022 MsaT006091.1:CDS
GCTGTATCTGATTCCAACTT+GGG 0.503244 2:+1480316 MsaT006091.1:CDS
AGGATTTGCAACCGCGGTGA+CGG 0.503756 2:-1479315 None:intergenic
ACGAGAATTACATGAGAAAA+CGG 0.506668 2:+1479774 MsaT006091.1:CDS
CTTAATGCTTATACAAGAGC+TGG 0.507717 2:+1480352 MsaT006091.1:CDS
CATGTTCAGTCTCAAAATCA+AGG 0.508213 2:+1480142 MsaT006091.1:CDS
AGCAGCAAACATACCCAAGT+TGG 0.510675 2:-1480329 None:intergenic
ACCATCACAGCATTTGGGAT+TGG 0.511721 2:+1480294 MsaT006091.1:CDS
GTTCCAGCTTCTGGTGGTGG+TGG 0.513092 2:+1480217 MsaT006091.1:CDS
CCGGCGATCATCGCTGCCAC+CGG 0.513461 2:+1479497 MsaT006091.1:CDS
GTGAGGATGATTCTTGTTGG+TGG 0.513652 2:-1479022 None:intergenic
CAAAAGTTGACGGCCGTGGC+CGG 0.517328 2:+1479366 MsaT006091.1:CDS
CCGGTGGCAGCGATGATCGC+CGG 0.518014 2:-1479497 None:intergenic
CAAAATGAAAGTGGCGGTGA+TGG 0.520198 2:+1479833 MsaT006091.1:CDS
GTTGCTGTTGCTGTTGTTGG+TGG 0.522829 2:-1479709 None:intergenic
TGGTACGCAGCTTCTTCGTA+AGG 0.525067 2:-1479650 None:intergenic
TTCAATGTTCCAGCTTCTGG+TGG 0.527514 2:+1480211 MsaT006091.1:CDS
CACCAACAACAGGATGATAT+GGG 0.530307 2:+1479893 MsaT006091.1:CDS
CCGATGGTGAAACAATTGAA+TGG 0.532182 2:+1479456 MsaT006091.1:CDS
GCATTCGAATTCTCCGGCCA+CGG 0.535337 2:-1479379 None:intergenic
TGCTGATGATGAGAATTTGA+AGG 0.536098 2:-1479044 None:intergenic
TCGCCGGCATTCGAATTCTC+CGG 0.537835 2:-1479385 None:intergenic
AACAACAATCAAAATGAAAG+TGG 0.537872 2:+1479824 MsaT006091.1:CDS
CACACAAAAGTTGACGGCCG+TGG 0.538934 2:+1479362 MsaT006091.1:CDS
TGGAAGAGGAGGTTCAGATC+TGG 0.540562 2:-1479237 None:intergenic
TGTTGTTGGTGGTGTTGATG+AGG 0.542903 2:-1479698 None:intergenic
ACAGTGTAGAGCCGCTGCTA+CGG 0.544103 2:-1479565 None:intergenic
AGAAGGTTGAATATTGGAGA+TGG 0.548864 2:-1479114 None:intergenic
GACAGGCACACAAAAGTTGA+CGG 0.553080 2:+1479356 MsaT006091.1:CDS
TTGTTGGATACATCTGGAAG+AGG 0.554700 2:-1479251 None:intergenic
ACTCCCCGCTGTAGCCCACA+TGG 0.560090 2:-1480020 None:intergenic
GTGATGGCCTTCCATCAAAA+GGG 0.560570 2:-1479076 None:intergenic
AACAGCCATGTGGGCTACAG+CGG 0.560758 2:+1480015 MsaT006091.1:CDS
GTTGCCGGTAACATATTTGA+CGG 0.564703 2:-1479995 None:intergenic
CAAAACGATCCACCAAGGAC+AGG 0.572544 2:+1479339 MsaT006091.1:CDS
GATGATATGGGCGAAGGTGA+CGG 0.576236 2:+1479905 MsaT006091.1:CDS
TGGGATTGTTCCGGTGCCGG+TGG 0.577716 2:-1479513 None:intergenic
ATTTGCAACCGCGGTGACGG+CGG 0.577827 2:-1479312 None:intergenic
GTTGAATTTCAAGGTGGACG+TGG 0.582295 2:+1480244 MsaT006091.1:CDS
ACTCTTGCTGCACATGTCGC+CGG 0.582995 2:-1479401 None:intergenic
GTTGCAGCACCACCACTCAC+TGG 0.588807 2:-1480064 None:intergenic
TTCTCATCATCAGCAGCTTG+TGG 0.593589 2:+1479052 MsaT006091.1:CDS
TGGCCGGAGAATTCGAATGC+CGG 0.599117 2:+1479382 MsaT006091.1:CDS
GAGATGGAGCCCATAATGAA+AGG 0.601517 2:-1479098 None:intergenic
GAGCCCATAATGAAAGGTGA+TGG 0.605278 2:-1479092 None:intergenic
TTGGATACATCTGGAAGAGG+AGG 0.605386 2:-1479248 None:intergenic
TGAATGGCTTCTTCAACAAG+CGG 0.607294 2:+1479472 MsaT006091.1:CDS
ATGATGAGAATTTGAAGGTG+AGG 0.608155 2:-1479039 None:intergenic
GGCATGAAATGTAACTGAGA+GGG 0.612083 2:-1480184 None:intergenic
GCCAATCCCAAATGCTGTGA+TGG 0.613902 2:-1480295 None:intergenic
GAAGCTGGAACATTGAACCT+TGG 0.618817 2:-1480205 None:intergenic
GAGTGGAAGTACAATTTGGA+TGG 0.623430 2:+1480039 MsaT006091.1:CDS
CAAAACAACAAGCAGAAGAG+AGG 0.623869 2:+1478969 MsaT006091.1:CDS
GATATGGGCGAAGGTGACGG+TGG 0.625298 2:+1479908 MsaT006091.1:CDS
AGATCTGGTGGCAATTGCCA+GGG 0.625421 2:-1479222 None:intergenic
CCTCAACCTAATGATGAGAG+TGG 0.625980 2:+1480451 MsaT006091.1:CDS
CAACAGGATGATATGGGCGA+AGG 0.628122 2:+1479899 MsaT006091.1:CDS
AACTTCAACCGCCGTCACCG+CGG 0.629301 2:+1479304 MsaT006091.1:CDS
GAAAGTGGCGGTGATGGTGA+TGG 0.632003 2:+1479839 MsaT006091.1:CDS
AAGAGGAGGTTCAGATCTGG+TGG 0.633145 2:-1479234 None:intergenic
AACGTCTCACTCCGTAGCAG+CGG 0.639266 2:+1479554 MsaT006091.1:CDS
ACCCTAACAGAGGAGCGTGT+TGG 0.640297 2:-1479670 None:intergenic
TGAGCGTTGAGAAATTGGCT+GGG 0.640396 2:-1479532 None:intergenic
GAATTAGGCCACAAATCCGA+TGG 0.643115 2:+1479440 MsaT006091.1:CDS
AGCATCAATGAGGTGTTGAG+TGG 0.643841 2:-1479198 None:intergenic
CTGAGAGGAGCTGATTTCGA+CGG 0.644392 2:-1479596 None:intergenic
AACAATCAAAATGAAAGTGG+CGG 0.649002 2:+1479827 MsaT006091.1:CDS
GCATCAATGAGGTGTTGAGT+GGG 0.650961 2:-1479197 None:intergenic
GTTACCGGCAACAGCCATGT+GGG 0.654057 2:+1480006 MsaT006091.1:CDS
TGTTACCGGCAACAGCCATG+TGG 0.658913 2:+1480005 MsaT006091.1:CDS
GGTAGTAGATAGTCATTGTG+AGG 0.659915 2:-1480491 None:intergenic
GAATTTCAAGGTGGACGTGG+TGG 0.660159 2:+1480247 MsaT006091.1:CDS
TGGCATGAAATGTAACTGAG+AGG 0.665182 2:-1480185 None:intergenic
TCCTCCAAAACGATCCACCA+AGG 0.665860 2:+1479334 MsaT006091.1:CDS
CAGCCATGTGGGCTACAGCG+GGG 0.667527 2:+1480017 MsaT006091.1:CDS
AGAGGAGCTGATTTCGACGG+CGG 0.670392 2:-1479593 None:intergenic
AGACACAGACACAAAACATG+TGG 0.683636 2:+1480107 MsaT006091.1:CDS
TTGCCAGGGAAGCATCAATG+AGG 0.690178 2:-1479208 None:intergenic
ATTTGGATGGTACCAGTGAG+TGG 0.695345 2:+1480052 MsaT006091.1:CDS
TGAGGATGAAACCCTAACAG+AGG 0.710378 2:-1479680 None:intergenic
TGGATGGTACCAGTGAGTGG+TGG 0.715883 2:+1480055 MsaT006091.1:CDS
AGCGCGCTATGAAAACTGAG+AGG 0.721312 2:-1479611 None:intergenic
GGTGGTGTTGAATTTCAAGG+TGG 0.744371 2:+1480238 MsaT006091.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 1478951 1480501 1478951 ID=MsaG006091
Chr2 mRNA 1478951 1480501 1478951 ID=MsaT006091.1;Parent=MsaG006091
Chr2 exon 1478951 1480501 1478951 ID=MsaT006091.1.exon1;Parent=MsaT006091.1
Chr2 CDS 1478951 1480501 1478951 ID=cds.MsaT006091.1;Parent=MsaT006091.1
Gene Sequence

>MsaT006091.1

ATGGAACTATCAGATTTACAAAACAACAAGCAGAAGAGAGGCACCACCAATATTCATCCCCATCCCCACCACCACCAACAAGAATCATCCTCACCTTCAAATTCTCATCATCAGCAGCTTGTGGTCCCTTTTGATGGAAGGCCATCACCTTTCATTATGGGCTCCATCTCCAATATTCAACCTTCTTCAAACTCTTCTCCCTCCAATTCTTCTTCTTCTTCTTCAGCTTCAGCTTCAGCTTCTGCACCCACTCAACACCTCATTGATGCTTCCCTGGCAATTGCCACCAGATCTGAACCTCCTCTTCCAGATGTATCCAACAACAACAACAACCAAATTCAACCATTTTCTTCAACTTCAACCGCCGTCACCGCGGTTGCAAATCCTCCAAAACGATCCACCAAGGACAGGCACACAAAAGTTGACGGCCGTGGCCGGAGAATTCGAATGCCGGCGACATGTGCAGCAAGAGTTTTCCAGTTGACCAGAGAATTAGGCCACAAATCCGATGGTGAAACAATTGAATGGCTTCTTCAACAAGCGGAGCCGGCGATCATCGCTGCCACCGGCACCGGAACAATCCCAGCCAATTTCTCAACGCTCAACGTCTCACTCCGTAGCAGCGGCTCTACACTGTCTGCGCCGCCGTCGAAATCAGCTCCTCTCAGTTTTCATAGCGCGCTTGCACTGGCTCATCATCCTTACGAAGAAGCTGCGTACCAACACGCTCCTCTGTTAGGGTTTCATCCTCATCAACACCACCAACAACAGCAACAGCAACAACAACTGATGAACGCTGATCAAATTGGTGAAGCACTTTCAAACGAGAATTACATGAGAAAACGGTATAGAGAAGATCTTTTCAAAGATGATAACAACAATCAAAATGAAAGTGGCGGTGATGGTGATGGTTCTTCTTCTCCCAAAAGTTTCAAAACAAATCACCAACAACAGGATGATATGGGCGAAGGTGACGGTGGTGGAGGAGGTGGTGGTGGAGCTTCTTCTTCTTCTGTAGGTAATTCTTTTTCTGGACTTCTTCGACCGTCAAATATGTTACCGGCAACAGCCATGTGGGCTACAGCGGGGAGTGGAAGTACAATTTGGATGGTACCAGTGAGTGGTGGTGCTGCAACTTCTTCAAATTCAGAGACACAGACACAGACACAAAACATGTGGCCTTTTACACCTCATGTTCAGTCTCAAAATCAAGGCAACGCTGCTGCTGTAACTCCCTCTCAGTTACATTTCATGCCAAGGTTCAATGTTCCAGCTTCTGGTGGTGGTGGTGGTGTTGAATTTCAAGGTGGACGTGGTGGTTTACAATTGGGTTCAGCGTATCAACCATCACAGCATTTGGGATTGGCTGTATCTGATTCCAACTTGGGTATGTTTGCTGCTCTTAATGCTTATACAAGAGCTGGCCATTTCAACGTTAACAACTCTTCTGATCATCATCATCATCATCAACATTCAATGTCATTGGACTCTCATTCTCAACCTCAACCTAATGATGAGAGTGGAGAAGATGCTCCTAATACCTCACAATGA
Protein sequence

>MsaT006091.1

MELSDLQNNKQKRGTTNIHPHPHHHQQESSSPSNSHHQQLVVPFDGRPSPFIMGSISNIQPSSNSSPSNSSSSSSASASASAPTQHLIDASLAIATRSEPPLPDVSNNNNNQIQPFSSTSTAVTAVANPPKRSTKDRHTKVDGRGRRIRMPATCAARVFQLTRELGHKSDGETIEWLLQQAEPAIIAATGTGTIPANFSTLNVSLRSSGSTLSAPPSKSAPLSFHSALALAHHPYEEAAYQHAPLLGFHPHQHHQQQQQQQQLMNADQIGEALSNENYMRKRYREDLFKDDNNNQNESGGDGDGSSSPKSFKTNHQQQDDMGEGDGGGGGGGGASSSSVGNSFSGLLRPSNMLPATAMWATAGSGSTIWMVPVSGGAATSSNSETQTQTQNMWPFTPHVQSQNQGNAAAVTPSQLHFMPRFNVPASGGGGGVEFQGGRGGLQLGSAYQPSQHLGLAVSDSNLGMFAALNAYTRAGHFNVNNSSDHHHHHQHSMSLDSHSQPQPNDESGEDAPNTSQ*