AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006125

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006125 MtrunA17_Chr2g0276901 85.934 519 68 3 227 741 1 518 0.0 864
MsaG006125 MtrunA17_Chr2g0276901 74.483 290 68 3 558 844 254 540 1.45e-119 374
MsaG006125 MtrunA17_Chr2g0276891 75.397 252 51 4 1 250 1 243 9.52e-84 270
MsaG006125 MtrunA17_Chr2g0320801 38.728 173 90 4 183 348 9 172 2.24e-30 122
MsaG006125 MtrunA17_Chr7g0265751 34.921 189 104 5 176 348 1 186 2.97e-29 119
MsaG006125 MtrunA17_Chr1g0162821 38.728 173 90 4 183 348 9 172 5.45e-29 117
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006125 AT1G10320.2 52.608 441 151 9 4 409 34 451 9.27e-146 450
MsaG006125 AT1G10320.1 52.715 442 158 8 4 409 34 460 9.50e-146 450
MsaG006125 AT1G27650.1 38.690 168 97 4 183 348 9 172 1.23e-28 117
MsaG006125 AT5G42820.1 36.571 175 91 4 183 348 9 172 6.42e-28 115
MsaG006125 AT5G42820.2 36.571 175 91 4 183 348 9 172 6.42e-28 115
MsaG006125 AT1G27650.2 38.384 99 58 1 251 349 28 123 3.92e-16 79.7

Find 233 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGTTCTTCTTCTTGTTCT+AGG 0.136086 2:+1812065 None:intergenic
AAGGAGATGGTTGCTTTATT+TGG 0.208040 2:-1808602 MsaT006125.1:CDS
ATCCTTTCCTTTGGCTACTA+TGG 0.214301 2:+1806948 None:intergenic
TCTCCTTTACCCAAAATTTC+GGG 0.233836 2:+1808618 None:intergenic
ATTGAAATACCAGAAGAAAT+TGG 0.246434 2:-1806462 MsaT006125.1:CDS
ATCTCCTTTACCCAAAATTT+CGG 0.247747 2:+1808617 None:intergenic
ATAGTCCTCCGGAGATTATT+TGG 0.251192 2:-1811964 MsaT006125.1:CDS
CTTGCTTATAACTCAGTTAA+TGG 0.266838 2:-1809812 MsaT006125.1:CDS
ACCAGTTCAGTTCCGAAATT+AGG 0.269563 2:+1811683 None:intergenic
AACCTCTTTCTCTCCTCTTC+AGG 0.274144 2:+1812187 None:intergenic
ATCCTTTCCTTCGGCTACTA+TGG 0.283540 2:+1807431 None:intergenic
CTAGGAAAAGCTCATTTAAT+AGG 0.296144 2:-1807642 MsaT006125.1:CDS
TGGTGGAATCCAATTTCTTC+TGG 0.297692 2:+1806453 None:intergenic
CACACAGAGTTCCTGAAGTT+TGG 0.301761 2:-1810038 MsaT006125.1:CDS
TCACAATTTCACCAAACTTC+AGG 0.309391 2:+1810027 None:intergenic
TTCATGGTCTGTATCTTTGC+CGG 0.317844 2:+1806877 None:intergenic
GTTAATGGTCGCTACTTTGC+TGG 0.323946 2:-1809797 MsaT006125.1:CDS
ATTCTTTCATTTGGCTACTA+AGG 0.330531 2:+1806705 None:intergenic
TGGAGGAGAAGAAGGGGATT+GGG 0.331261 2:+1811893 None:intergenic
GATGTTTCAATCCTTTCCTT+TGG 0.335919 2:+1806939 None:intergenic
ACCTGTCAGAATCTGTTTCT+AGG 0.342568 2:+1808510 None:intergenic
TAGATAGGGTTACTATAAAC+AGG 0.344706 2:-1807576 MsaT006125.1:CDS
ACTCAAGAAGATCAAGATCT+AGG 0.351478 2:-1807861 MsaT006125.1:CDS
GCTCAACAACTACCTAATTT+CGG 0.351598 2:-1811695 MsaT006125.1:CDS
GATGTCTAGATTCTTTCATT+TGG 0.352316 2:+1806696 None:intergenic
TTTGTATGTCTATCCTCATC+TGG 0.352950 2:+1806977 None:intergenic
AAGAGTATTCTGATGTAGAT+AGG 0.365009 2:-1807591 MsaT006125.1:CDS
ATCTGTTTCTAGGATATTGC+TGG 0.365763 2:+1808520 None:intergenic
AGGGAGATGGGTCAATTAAA+TGG 0.367639 2:-1807841 MsaT006125.1:CDS
CCACATCAGAATACTCTTCA+TGG 0.370587 2:+1807344 None:intergenic
CCACATCAGAATACTCTTCA+TGG 0.370969 2:+1806861 None:intergenic
AAGGGTTAGAAGAGGTAGAT+AGG 0.372781 2:-1807684 MsaT006125.1:CDS
GAGAAGGGTGCAGGACTTAT+CGG 0.373402 2:+1811504 None:intergenic
GGAACTCACAAAAGTGGATC+TGG 0.378231 2:-1807522 MsaT006125.1:CDS
ATGAGGATAGACATAGAAAA+AGG 0.379408 2:-1807213 MsaT006125.1:CDS
GAGGTTGAGAGGGAGATTCC+GGG 0.379436 2:+1811729 None:intergenic
AGAGGTTGAGAGGGAGATTC+CGG 0.382974 2:+1811728 None:intergenic
AACGAAAATGATAAAGTTGA+TGG 0.383358 2:-1806539 MsaT006125.1:CDS
TTGGTATTCCATTCCTCATC+AGG 0.390772 2:+1807769 None:intergenic
ACCTAATTTCGGAACTGAAC+TGG 0.393701 2:-1811684 MsaT006125.1:intron
CATAGTCTCCGCCAGGATTA+CGG 0.395908 2:+1808666 None:intergenic
ATGAGGATAGACATACAAAA+AGG 0.397308 2:-1806973 MsaT006125.1:CDS
ATACCTGGTGCTGGTGGTTG+TGG 0.397792 2:+1811873 None:intergenic
TGTTCTTGTCTGTCTTCGCT+TGG 0.399558 2:+1806407 None:intergenic
TTTATGATTGTATTTAAAGT+TGG 0.404123 2:+1807750 None:intergenic
GTGGAGGAGAAGAAGGGGAT+TGG 0.407602 2:+1811892 None:intergenic
GGAGAGAAAGAGGTTACTAT+TGG 0.412207 2:-1812179 MsaT006125.1:CDS
TCGCTACTTTGCTGGGAAAC+AGG 0.416456 2:-1809789 MsaT006125.1:intron
GATTGCTGAGAAGGAACGTT+TGG 0.417206 2:-1812233 MsaT006125.1:CDS
AGAACTCACATAAGAGGAAT+GGG 0.417356 2:-1806787 MsaT006125.1:CDS
GTTCTTGTCTGTCTTCGCTT+GGG 0.423470 2:+1806408 None:intergenic
ATCCTTTCCTTTGGCTACCA+TGG 0.425229 2:+1807188 None:intergenic
GATGTCTTGATCCTTTCCTT+TGG 0.427167 2:+1807179 None:intergenic
AAAACTGCAGCATGTCGTTT+CGG 0.428061 2:-1811555 MsaT006125.1:CDS
AGATGGAAGGTTGCCATTTG+TGG 0.428325 2:-1808958 MsaT006125.1:CDS
CTGATCCAGCCTCATCAATC+TGG 0.433829 2:+1806753 None:intergenic
CTACGTGCAGTATAAGTTGT+TGG 0.433986 2:-1809846 MsaT006125.1:CDS
GGTGGTTGTGGAGGAGAAGA+AGG 0.435899 2:+1811885 None:intergenic
ATGAGTATTCTGACGTGGAT+TGG 0.437098 2:-1807099 MsaT006125.1:CDS
TGAGTATTCTGACGTGGATT+GGG 0.439025 2:-1807098 MsaT006125.1:CDS
TGTGGCGAGTATATGAAATC+AGG 0.441103 2:-1808940 MsaT006125.1:CDS
AAAAGTGGAGCTGGGGTAGT+AGG 0.443968 2:-1807021 MsaT006125.1:CDS
TCGTTCTAGTAGAAGAAAAC+AGG 0.444811 2:-1807815 MsaT006125.1:CDS
CAGGACTTATCGGGTAGAAA+TGG 0.450797 2:+1811514 None:intergenic
ATTCACTGTTTCCGTAATCC+TGG 0.453715 2:-1808677 MsaT006125.1:CDS
ATTGAAGAAATTGAAGAGAA+AGG 0.454676 2:-1812269 MsaT006125.1:CDS
CTTCTGATTCTGATTCTGAC+AGG 0.458819 2:-1807708 MsaT006125.1:CDS
CAATGGTCCTGGACTTGCTT+GGG 0.459683 2:-1811467 MsaT006125.1:CDS
GAAAAGCAGCATGGCAGTAA+AGG 0.462211 2:-1807295 MsaT006125.1:CDS
AGTGGATCTGGGATAGTAGT+AGG 0.462625 2:-1807510 MsaT006125.1:CDS
ATAAGAGGAATGGGGATAGT+AGG 0.462830 2:-1806778 MsaT006125.1:CDS
CTGGTTCAGCCTCATGAATC+CGG 0.463492 2:+1806996 None:intergenic
CAAAGCGGGGACAGCTGTTA+CGG 0.465797 2:-1806674 MsaT006125.1:CDS
TCGAAGAGAGGGAGAAAGCT+TGG 0.466922 2:-1812114 MsaT006125.1:CDS
GTTTCGGTGACCGATGTAGC+AGG 0.471525 2:-1811539 MsaT006125.1:CDS
AGAAATGGACCCTGCTACAT+CGG 0.473301 2:+1811529 None:intergenic
GTAACTGATAAATCTGAATC+CGG 0.474468 2:-1806896 MsaT006125.1:CDS
TGACAAAGATAGAGGAGAGA+TGG 0.476773 2:-1806639 MsaT006125.1:CDS
TGGTGAAATTGTGAACTTCA+AGG 0.477712 2:-1810018 MsaT006125.1:intron
GGAGGAGAAGAAGGGGATTG+GGG 0.478173 2:+1811894 None:intergenic
AAGGATCAAGACATCGGAGC+AGG 0.483365 2:-1807171 MsaT006125.1:CDS
GAAAAGCAGCATGATAGAAA+AGG 0.484957 2:-1807547 MsaT006125.1:CDS
AAGGAAAGAACTCACATAAG+AGG 0.486874 2:-1806793 MsaT006125.1:CDS
GGAACTCACAAAAGTGGAGC+TGG 0.487506 2:-1807030 MsaT006125.1:CDS
TAAAATTGCAAGCTGTTCCA+TGG 0.487680 2:+1808699 None:intergenic
CTGATCCAGCCTCATAAATC+CGG 0.487762 2:+1807236 None:intergenic
CTGATAATTGTGAGATGTCA+AGG 0.489784 2:-1808574 MsaT006125.1:CDS
ACGAAAATGATAAAGTTGAT+GGG 0.490047 2:-1806538 MsaT006125.1:CDS
GATGTCTCGATCCTTTCCTT+CGG 0.490801 2:+1807422 None:intergenic
AATTTGTTAATTTAACAAGA+TGG 0.492703 2:-1808975 MsaT006125.1:CDS
AAGGAAAGAGATTGCTGAGA+AGG 0.497171 2:-1812242 MsaT006125.1:CDS
ATCCTGGCGGAGACTATGAA+TGG 0.498169 2:-1808661 MsaT006125.1:CDS
AGAAGGGTGCAGGACTTATC+GGG 0.501162 2:+1811505 None:intergenic
GTGGTTGTGGAGGAGAAGAA+GGG 0.501963 2:+1811886 None:intergenic
TTAATGGTCGCTACTTTGCT+GGG 0.503154 2:-1809796 MsaT006125.1:CDS
CTCAAGAAGATCAAGATCTA+GGG 0.503633 2:-1807860 MsaT006125.1:CDS
GACAAAGATAGAGGAGAGAT+GGG 0.504372 2:-1806638 MsaT006125.1:CDS
TGCTGAGAAGGAACGTTTGG+AGG 0.505737 2:-1812230 MsaT006125.1:CDS
AAGGATTGAAACATCAGAGC+AGG 0.510912 2:-1806931 MsaT006125.1:CDS
AAAGGAAGGAACTCGCAAAA+GGG 0.512313 2:-1807277 MsaT006125.1:CDS
AAGAGTATTCTGATGTGGAT+TGG 0.513319 2:-1806856 MsaT006125.1:CDS
AAGAGTATTCTGATGTGGAT+TGG 0.513319 2:-1807339 MsaT006125.1:CDS
TAAAGGAAGGAACTCGCAAA+AGG 0.514540 2:-1807278 MsaT006125.1:CDS
AGAGTATTCTGATGTGGATT+GGG 0.515015 2:-1806855 MsaT006125.1:CDS
AGAGTATTCTGATGTGGATT+GGG 0.515015 2:-1807338 MsaT006125.1:CDS
CTTGCAAGACAACTCTCCAT+TGG 0.516208 2:+1806433 None:intergenic
TCTGAATCTGACAAAGATAG+AGG 0.517598 2:-1807367 MsaT006125.1:CDS
AAGATCAAGATCTAGGGAGA+TGG 0.519290 2:-1807854 MsaT006125.1:CDS
GATGAATCTGACAAAGATAG+AGG 0.520588 2:-1806647 MsaT006125.1:CDS
TTTCATATACTCGCCACAAA+TGG 0.520774 2:+1808945 None:intergenic
AATTGAAGAGAAAGGAGAGA+AGG 0.521748 2:-1812261 MsaT006125.1:CDS
AACATTGTGCAGAGGTTGAG+AGG 0.522448 2:+1811718 None:intergenic
AAGAAATTGGATTCCACCAA+TGG 0.523668 2:-1806449 MsaT006125.1:CDS
ATCCTGAAGAGGAGAGAAAG+AGG 0.523772 2:-1812189 MsaT006125.1:CDS
AAGAACTCACATAAGAGGAA+TGG 0.525932 2:-1806788 MsaT006125.1:CDS
TGTGAGATGTCAAGGGTCCA+TGG 0.526249 2:-1808566 MsaT006125.1:CDS
TCCTAGAAACAGATTCTGAC+AGG 0.529003 2:-1808511 MsaT006125.1:intron
AGGAGCTGGATTCATGAGGC+TGG 0.530130 2:-1807490 MsaT006125.1:CDS
TCTTGTTCCCAAGCAAGTCC+AGG 0.531043 2:+1811460 None:intergenic
TGTTAATTTAACAAGATGGA+AGG 0.531596 2:-1808971 MsaT006125.1:CDS
GCCCATTCATAGTCTCCGCC+AGG 0.533903 2:+1808659 None:intergenic
TGGATTGGGATACTATGAGT+AGG 0.534110 2:-1807324 MsaT006125.1:CDS
CGAAAATGATAAAGTTGATG+GGG 0.535498 2:-1806537 MsaT006125.1:CDS
TTTCCTAGCCAAATAATCTC+CGG 0.539571 2:+1811956 None:intergenic
ACAATCATAAAAGAAAATAC+AGG 0.539712 2:-1807738 MsaT006125.1:CDS
GATTCTGATTCTGACAGGGA+AGG 0.541505 2:-1807703 MsaT006125.1:CDS
AAGGAAGGAACTCACAAAAG+TGG 0.543036 2:-1807528 MsaT006125.1:CDS
TCAGCGTGCTAAAATTTCCA+TGG 0.545766 2:+1808549 None:intergenic
AGAGTATTCTGATGTAGATA+GGG 0.548177 2:-1807590 MsaT006125.1:CDS
AAGAATCTAGACATCAAAGC+GGG 0.548850 2:-1806688 MsaT006125.1:CDS
AGATCAAGATCTAGGGAGAT+GGG 0.548935 2:-1807853 MsaT006125.1:CDS
AGGTACCAGATTGATGAGGC+TGG 0.549430 2:-1806758 MsaT006125.1:CDS
GCAAGACAACTCTCCATTGG+TGG 0.550205 2:+1806436 None:intergenic
ATAACATATATACCTGGTGC+TGG 0.550671 2:+1811864 None:intergenic
AGATACCGGATTTATGAGGC+TGG 0.552003 2:-1807241 MsaT006125.1:CDS
GATGAGGATAGAGATGGAGA+TGG 0.553244 2:-1807463 MsaT006125.1:CDS
GAGTCTGAATCTGACAAAGA+TGG 0.554962 2:-1807127 MsaT006125.1:CDS
CACCATAGTAGCCGAAGGAA+AGG 0.557409 2:-1807433 MsaT006125.1:CDS
CTTGCTTGGGAACAAGATCA+AGG 0.558188 2:-1811454 MsaT006125.1:CDS
GAACTCACAAAAGTGGATCT+GGG 0.558394 2:-1807521 MsaT006125.1:CDS
TCAGAGCAGGGACAACAGTA+AGG 0.560225 2:-1806918 MsaT006125.1:CDS
AAGGAAGGAACTCACAAAAG+TGG 0.560430 2:-1807036 MsaT006125.1:CDS
TGATGATGATGATAGTCCTC+CGG 0.562508 2:-1811975 MsaT006125.1:CDS
TCCTGGCGGAGACTATGAAT+GGG 0.563105 2:-1808660 MsaT006125.1:CDS
TAGTAGATACCGGATTTATG+AGG 0.563672 2:-1807245 MsaT006125.1:CDS
GGAGCTGGGGTAGTAGGTAC+CGG 0.564663 2:-1807015 MsaT006125.1:CDS
CCTCCGGAGATTATTTGGCT+AGG 0.566962 2:-1811959 MsaT006125.1:CDS
AAAATTGCAAGCTGTTCCAT+GGG 0.569618 2:+1808700 None:intergenic
CCTGGTGCTGGTGGTTGTGG+AGG 0.569821 2:+1811876 None:intergenic
GAACTCACAAAAGTGGAGCT+GGG 0.570738 2:-1807029 MsaT006125.1:CDS
CTCCTTTACCCAAAATTTCG+GGG 0.572507 2:+1808619 None:intergenic
AAAGAACTCCTGATGAGGAA+TGG 0.572610 2:-1807777 MsaT006125.1:CDS
TACATCAAATCCCTTTCCCC+CGG 0.572952 2:-1811747 MsaT006125.1:CDS
TCTGGGATAGTAGTAGGAGC+TGG 0.573131 2:-1807504 MsaT006125.1:CDS
CCTCCACAACCACCAGCACC+AGG 0.573575 2:-1811876 MsaT006125.1:intron
ACAATGGTCCTGGACTTGCT+TGG 0.574271 2:-1811468 MsaT006125.1:CDS
GAGCACAACATGATTACTCT+AGG 0.578647 2:-1806616 MsaT006125.1:CDS
ATTCTGATTCTGACAGGGAA+GGG 0.580207 2:-1807702 MsaT006125.1:CDS
AGCAGCATGGCAGTAAAGGA+AGG 0.581903 2:-1807291 MsaT006125.1:CDS
AGGCAGGCAGACAGACCTCA+AGG 0.583368 2:+1811431 None:intergenic
AAGGATCGAGACATCAGAGA+AGG 0.586354 2:-1807414 MsaT006125.1:CDS
GACAGACAAGAACAAAGCAA+AGG 0.587753 2:-1806398 MsaT006125.1:CDS
TGGATTGGGATACTATGAGT+AGG 0.589392 2:-1807084 MsaT006125.1:CDS
AGGTTGAGAGGGAGATTCCG+GGG 0.591558 2:+1811730 None:intergenic
GAAAAGCAGCATGACAGCAA+AGG 0.591677 2:-1806812 MsaT006125.1:CDS
GGAGATGTGGTCGAATCGAA+AGG 0.592536 2:-1812305 None:intergenic
AGAGGCGGAGAGAATCGAAA+GGG 0.592841 2:-1812149 MsaT006125.1:CDS
TCGACTCAACGATCCTGAAG+AGG 0.592991 2:-1812200 MsaT006125.1:CDS
ACATTGTGCAGAGGTTGAGA+GGG 0.594548 2:+1811719 None:intergenic
ATAGAATCGCTTTCGAAGAG+AGG 0.598605 2:-1812126 MsaT006125.1:CDS
GGATTGGGATACTATGAGTA+GGG 0.598777 2:-1807323 MsaT006125.1:CDS
GGATTGGGATACTATGAGTA+GGG 0.599782 2:-1807083 MsaT006125.1:CDS
TAGAATCGCTTTCGAAGAGA+GGG 0.601888 2:-1812125 MsaT006125.1:CDS
GGAACAGCGATAGTAGATAC+CGG 0.602268 2:-1807255 MsaT006125.1:CDS
TTCTGATTCTGATTCTGACA+GGG 0.603484 2:-1807707 MsaT006125.1:CDS
GCACGTAGACATTTCCTCGC+AGG 0.603491 2:+1809861 None:intergenic
TGGTTGTGGAGGAGAAGAAG+GGG 0.606296 2:+1811887 None:intergenic
GTATATGAAATCAGGTTACA+AGG 0.607083 2:-1808932 MsaT006125.1:intron
GGAACAAGATCAAGGCCTTG+AGG 0.607210 2:-1811446 MsaT006125.1:CDS
CACCATAGTAGCCAAAGGAA+AGG 0.607686 2:-1806950 MsaT006125.1:CDS
CACCATGGTAGCCAAAGGAA+AGG 0.609982 2:-1807190 MsaT006125.1:CDS
TGGATTGGGATACTACGAGT+AGG 0.611788 2:-1806841 MsaT006125.1:CDS
AGCAGCATGACAGCAAAGGA+AGG 0.612309 2:-1807051 MsaT006125.1:CDS
CCATGAAGAGTATTCTGATG+TGG 0.613223 2:-1806861 MsaT006125.1:CDS
CCATGAAGAGTATTCTGATG+TGG 0.613223 2:-1807344 MsaT006125.1:CDS
AAGAGGCGGAGAGAATCGAA+AGG 0.615210 2:-1812150 MsaT006125.1:CDS
GTTACTATTGGAGCAGCAAG+AGG 0.616498 2:-1812167 MsaT006125.1:CDS
TCTTAACAGACTTGTTCCCA+TGG 0.621930 2:-1808716 MsaT006125.1:intron
GGATGTGATGAGGATAGAGA+TGG 0.622071 2:-1807469 MsaT006125.1:CDS
AAAAGTATCATGAACACACT+AGG 0.623161 2:-1807660 MsaT006125.1:CDS
GGATTGGGATACTACGAGTA+GGG 0.623278 2:-1806840 MsaT006125.1:CDS
CACTGTTTCCGTAATCCTGG+CGG 0.623496 2:-1808674 MsaT006125.1:CDS
AAAGCAAAGAACTCCTGATG+AGG 0.624280 2:-1807782 MsaT006125.1:CDS
GAAAATGATAAAGTTGATGG+GGG 0.626135 2:-1806536 MsaT006125.1:CDS
TGATGAGGCTGGATCAGATG+AGG 0.627514 2:-1806747 MsaT006125.1:CDS
TCCTTTACCCAAAATTTCGG+GGG 0.628973 2:+1808620 None:intergenic
AATGGTTCATTTCACCTGCG+AGG 0.632032 2:-1809875 MsaT006125.1:CDS
TGACAGGGAAGGGTTAGAAG+AGG 0.632794 2:-1807692 MsaT006125.1:CDS
AGGATCAAGACATCGGAGCA+GGG 0.633206 2:-1807170 MsaT006125.1:CDS
AAGGAAGGAACTCGCAAAAG+GGG 0.633299 2:-1807276 MsaT006125.1:CDS
AACTCACAAAAGTGGAGCTG+GGG 0.634655 2:-1807028 MsaT006125.1:CDS
AAAGGCACCATGGTAGCCAA+AGG 0.634673 2:-1807195 MsaT006125.1:CDS
AGGATCGAGACATCAGAGAA+GGG 0.634774 2:-1807413 MsaT006125.1:CDS
GTAAAGCAGCATGACAGCAA+AGG 0.637596 2:-1807055 MsaT006125.1:CDS
TTATGAGGCTGGATCAGATG+AGG 0.638298 2:-1807230 MsaT006125.1:CDS
TGATAATTGTGAGATGTCAA+GGG 0.643511 2:-1808573 MsaT006125.1:CDS
TTCTAGTAGAAGAAAACAGG+AGG 0.644725 2:-1807812 MsaT006125.1:CDS
AGCAGCATGATAGAAAAGGA+AGG 0.644760 2:-1807543 MsaT006125.1:CDS
TCATGAGGCTGAACCAGATG+AGG 0.645107 2:-1806990 MsaT006125.1:CDS
GGTCGAATCGAAAGGAGAAG+AGG 0.646281 2:-1812297 MsaT006125.1:CDS
TTTCGGTGACCGATGTAGCA+GGG 0.649087 2:-1811538 MsaT006125.1:CDS
AAAGGAAAGGATCAAGACAT+CGG 0.653130 2:-1807177 MsaT006125.1:CDS
GAAGACACCATAGTAGCCGA+AGG 0.653894 2:-1807438 MsaT006125.1:CDS
AAAGGCACCATAGTAGCCAA+AGG 0.654107 2:-1806955 MsaT006125.1:CDS
AGGATTGAAACATCAGAGCA+GGG 0.658719 2:-1806930 MsaT006125.1:CDS
TAGTAGGAGCTGGATTCATG+AGG 0.658776 2:-1807494 MsaT006125.1:CDS
GAATCGAAAGGAGAAGAGGA+AGG 0.658952 2:-1812293 MsaT006125.1:CDS
AAGCTCATTTAATAGGAACA+AGG 0.661186 2:-1807635 MsaT006125.1:CDS
ACTATTGGAGCAGCAAGAGG+CGG 0.664869 2:-1812164 MsaT006125.1:CDS
GGTTGAGAGGGAGATTCCGG+GGG 0.671144 2:+1811731 None:intergenic
GAACTCACATAAGAGGAATG+GGG 0.673945 2:-1806786 MsaT006125.1:CDS
TCTGAATCTGACAAAGATGG+AGG 0.674663 2:-1807124 MsaT006125.1:CDS
TAGTAGGTACCGGATTCATG+AGG 0.677752 2:-1807005 MsaT006125.1:CDS
CCTAGCCAAATAATCTCCGG+AGG 0.678025 2:+1811959 None:intergenic
TGTTGAGCAACATTGTGCAG+AGG 0.684405 2:+1811710 None:intergenic
TAGTAGGTACCAGATTGATG+AGG 0.688357 2:-1806762 MsaT006125.1:CDS
TCATGAGGCTGGATGTGATG+AGG 0.692514 2:-1807479 MsaT006125.1:CDS
AGGGACAATGAAAAGCAGCA+TGG 0.694028 2:-1807304 MsaT006125.1:CDS
AGATAGGGTTACTATAAACA+GGG 0.714784 2:-1807575 MsaT006125.1:CDS
AAAGAATCTAGACATCAAAG+CGG 0.719311 2:-1806689 MsaT006125.1:CDS
AGAATCTAGACATCAAAGCG+GGG 0.720964 2:-1806687 MsaT006125.1:CDS
ACATATATACCTGGTGCTGG+TGG 0.722080 2:+1811867 None:intergenic
AGGACATGAGTATTCTGACG+TGG 0.730553 2:-1807104 MsaT006125.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 1806379 1812323 1806379 ID=MsaG006125
Chr2 mRNA 1806379 1812323 1806379 ID=MsaT006125.1;Parent=MsaG006125
Chr2 exon 1806379 1807888 1806379 ID=MsaT006125.1.exon8;Parent=MsaT006125.1
Chr2 CDS 1806379 1807888 1806379 ID=cds.MsaT006125.1;Parent=MsaT006125.1
Chr2 exon 1808512 1808729 1808512 ID=MsaT006125.1.exon7;Parent=MsaT006125.1
Chr2 CDS 1808512 1808729 1808512 ID=cds.MsaT006125.1;Parent=MsaT006125.1
Chr2 exon 1808933 1809007 1808933 ID=MsaT006125.1.exon6;Parent=MsaT006125.1
Chr2 CDS 1808933 1809007 1808933 ID=cds.MsaT006125.1;Parent=MsaT006125.1
Chr2 exon 1809790 1809906 1809790 ID=MsaT006125.1.exon5;Parent=MsaT006125.1
Chr2 CDS 1809790 1809906 1809790 ID=cds.MsaT006125.1;Parent=MsaT006125.1
Chr2 exon 1810019 1810111 1810019 ID=MsaT006125.1.exon4;Parent=MsaT006125.1
Chr2 CDS 1810019 1810111 1810019 ID=cds.MsaT006125.1;Parent=MsaT006125.1
Chr2 exon 1811443 1811604 1811443 ID=MsaT006125.1.exon3;Parent=MsaT006125.1
Chr2 CDS 1811443 1811604 1811443 ID=cds.MsaT006125.1;Parent=MsaT006125.1
Chr2 exon 1811685 1811798 1811685 ID=MsaT006125.1.exon2;Parent=MsaT006125.1
Chr2 CDS 1811685 1811798 1811685 ID=cds.MsaT006125.1;Parent=MsaT006125.1
Chr2 exon 1811877 1812323 1811877 ID=MsaT006125.1.exon1;Parent=MsaT006125.1
Chr2 CDS 1811877 1812323 1811877 ID=cds.MsaT006125.1;Parent=MsaT006125.1
Gene Sequence

>MsaT006125.1

ATGTGGTCGAATCGAAAGGAGAAGAGGAAGGCATTGAAGAAATTGAAGAGAAAGGAGAGAAGGAAAGAGATTGCTGAGAAGGAACGTTTGGAGGAAGAAGCTCGACTCAACGATCCTGAAGAGGAGAGAAAGAGGTTACTATTGGAGCAGCAAGAGGCGGAGAGAATCGAAAGGGATAGAATCGCTTTCGAAGAGAGGGAGAAAGCTTGGATTATCAAACAACAACAACAACGAGACCTAGAACAAGAAGAAGAACAAGAACTATCTCAACGAGACGATATCAAAGAAGAAGATGATAAATCAGAATTAGAAGAAGAAGAAGAAGATGATGATGATGATAGTCCTCCGGAGATTATTTGGCTAGGAAATGAAATTATCATCAAAAAGAAGAAAAAGAAAAAGCTGAACCCCAATCCCCTTCTTCTCCTCCACAACCACCAGCACCAGGAGCAGAATGAGCATCATGATAATAGACCTACATCAAATCCCTTTCCCCCGGAATCTCCCTCTCAACCTCTGCACAATGTTGCTCAACAACTACCTAATTTCGGAACTGAACTGGATAAAGCTCACTGTCCCTTTCATCTTAAAACTGCAGCATGTCGTTTCGGTGACCGATGTAGCAGGGTCCATTTCTACCCGATAAGTCCTGCACCCTTCTCATCAAAAACATGTACAATGGTCCTGGACTTGCTTGGGAACAAGATCAAGGCCTTGAGGTCTTATACAGATGAGGAGGTTGAACGCTGTTTTGAAGAGTTTTATGAAGATGTTCACACAGAGTTCCTGAAGTTTGGTGAAATTGTGAACTTCAAGGTGTGTAAAAATGGTTCATTTCACCTGCGAGGAAATGTCTACGTGCAGTATAAGTTGTTGGATTCTGCTCTGCTTGCTTATAACTCAGTTAATGGTCGCTACTTTGCTGGGAAACAGGTGAGCTGTAAATTTGTTAATTTAACAAGATGGAAGGTTGCCATTTGTGGCGAGTATATGAAATCAGGTTACAAGACTTGTTCCCATGGAACAGCTTGCAATTTTATTCACTGTTTCCGTAATCCTGGCGGAGACTATGAATGGGCAGATTCTGATAAACCACCCCCGAAATTTTGGGTAAAGGAGATGGTTGCTTTATTTGGTCATTCTGATAATTGTGAGATGTCAAGGGTCCATGGAAATTTTAGCACGCTGAAGCACTCCAGCAATATCCTAGAAACAGATTCTGACAGGTATCACTCAAGAAGATCAAGATCTAGGGAGATGGGTCAATTAAATGGTAGTCGTTCTAGTAGAAGAAAACAGGAGGATGAAAGAAAGCAAAGAACTCCTGATGAGGAATGGAATACCAACTTTAAATACAATCATAAAAGAAAATACAGGAACAAAACTTCTGATTCTGATTCTGACAGGGAAGGGTTAGAAGAGGTAGATAGGAAAAAGTATCATGAACACACTAGGAAAAGCTCATTTAATAGGAACAAGGATGACAATAGCAGAAAGCATGAAGAGTATTCTGATGTAGATAGGGTTACTATAAACAGGGACAATGAAAAGCAGCATGATAGAAAAGGAAGGAACTCACAAAAGTGGATCTGGGATAGTAGTAGGAGCTGGATTCATGAGGCTGGATGTGATGAGGATAGAGATGGAGATGGAAGAAGACACCATAGTAGCCGAAGGAAAGGATCGAGACATCAGAGAAGGGACAGCAGCAATGTAACTGATGAATCTGAATCTGACAAAGATAGAGGCCATGAAGAGTATTCTGATGTGGATTGGGATACTATGAGTAGGGACAATGAAAAGCAGCATGGCAGTAAAGGAAGGAACTCGCAAAAGGGGAACAGCGATAGTAGATACCGGATTTATGAGGCTGGATCAGATGAGGATAGACATAGAAAAAGGCACCATGGTAGCCAAAGGAAAGGATCAAGACATCGGAGCAGGGACAGCAGTAATGTAGCTGACGAGTCTGAATCTGACAAAGATGGAGGACATGAGTATTCTGACGTGGATTGGGATACTATGAGTAGGGACAACGTAAAGCAGCATGACAGCAAAGGAAGGAACTCACAAAAGTGGAGCTGGGGTAGTAGGTACCGGATTCATGAGGCTGAACCAGATGAGGATAGACATACAAAAAGGCACCATAGTAGCCAAAGGAAAGGATTGAAACATCAGAGCAGGGACAACAGTAAGGTAACTGATAAATCTGAATCCGGCAAAGATACAGACCATGAAGAGTATTCTGATGTGGATTGGGATACTACGAGTAGGGACAATGAAAAGCAGCATGACAGCAAAGGAAAGAACTCACATAAGAGGAATGGGGATAGTAGGTACCAGATTGATGAGGCTGGATCAGATGAGGATAGACATAGAAAAAAGCACCTTAGTAGCCAAATGAAAGAATCTAGACATCAAAGCGGGGACAGCTGTTACGGAATCGATGAATCTGACAAAGATAGAGGAGAGATGGGAGCACAACATGATTACTCTAGGAAAAGCTCGAGACACAAAAGATCTGATCTTCGAGACTACTGTGAAGAGTATGAAAACGAAAATGATAAAGTTGATGGGGGTTGGTCTCAGAGAGACAGTGACCGACGAGCACATCATCACAAAAAGAAGAGATTGAAATACCAGAAGAAATTGGATTCCACCAATGGAGAGTTGTCTTGCAAGAACCCAAGCGAAGACAGACAAGAACAAAGCAAAGGAAGTGACAAACACTTATAA
Protein sequence

>MsaT006125.1

MWSNRKEKRKALKKLKRKERRKEIAEKERLEEEARLNDPEEERKRLLLEQQEAERIERDRIAFEEREKAWIIKQQQQRDLEQEEEQELSQRDDIKEEDDKSELEEEEEDDDDDSPPEIIWLGNEIIIKKKKKKKLNPNPLLLLHNHQHQEQNEHHDNRPTSNPFPPESPSQPLHNVAQQLPNFGTELDKAHCPFHLKTAACRFGDRCSRVHFYPISPAPFSSKTCTMVLDLLGNKIKALRSYTDEEVERCFEEFYEDVHTEFLKFGEIVNFKVCKNGSFHLRGNVYVQYKLLDSALLAYNSVNGRYFAGKQVSCKFVNLTRWKVAICGEYMKSGYKTCSHGTACNFIHCFRNPGGDYEWADSDKPPPKFWVKEMVALFGHSDNCEMSRVHGNFSTLKHSSNILETDSDRYHSRRSRSREMGQLNGSRSSRRKQEDERKQRTPDEEWNTNFKYNHKRKYRNKTSDSDSDREGLEEVDRKKYHEHTRKSSFNRNKDDNSRKHEEYSDVDRVTINRDNEKQHDRKGRNSQKWIWDSSRSWIHEAGCDEDRDGDGRRHHSSRRKGSRHQRRDSSNVTDESESDKDRGHEEYSDVDWDTMSRDNEKQHGSKGRNSQKGNSDSRYRIYEAGSDEDRHRKRHHGSQRKGSRHRSRDSSNVADESESDKDGGHEYSDVDWDTMSRDNVKQHDSKGRNSQKWSWGSRYRIHEAEPDEDRHTKRHHSSQRKGLKHQSRDNSKVTDKSESGKDTDHEEYSDVDWDTTSRDNEKQHDSKGKNSHKRNGDSRYQIDEAGSDEDRHRKKHLSSQMKESRHQSGDSCYGIDESDKDRGEMGAQHDYSRKSSRHKRSDLRDYCEEYENENDKVDGGWSQRDSDRRAHHHKKKRLKYQKKLDSTNGELSCKNPSEDRQEQSKGSDKHL*