AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006130

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006130 MtrunA17_Chr2g0276841 98.429 700 10 1 1 699 1 700 0.0 1452
MsaG006130 MtrunA17_Chr2g0294841 58.133 664 258 5 41 694 22 675 0.0 810
MsaG006130 MtrunA17_Chr1g0198761 47.200 625 319 5 68 684 46 667 0.0 611
MsaG006130 MtrunA17_Chr2g0290391 43.109 624 335 5 69 680 51 666 0.0 533
MsaG006130 MtrunA17_Chr5g0431001 50.235 426 207 2 68 488 60 485 2.61e-154 456
MsaG006130 MtrunA17_Chr5g0448761 29.338 634 417 14 69 680 60 684 2.28e-79 269
MsaG006130 MtrunA17_Chr2g0329031 27.014 633 425 16 75 698 20 624 2.29e-74 254
MsaG006130 MtrunA17_Chr1g0202341 28.169 568 385 10 69 625 51 606 3.33e-71 244
MsaG006130 MtrunA17_Chr1g0207551 29.373 606 363 15 69 646 34 602 5.79e-71 246
MsaG006130 MtrunA17_Chr5g0440451 29.310 580 362 12 69 623 43 599 7.64e-67 237
MsaG006130 MtrunA17_Chr3g0082121 28.042 567 380 13 67 625 41 587 1.56e-62 221
MsaG006130 MtrunA17_Chr2g0296781 28.152 579 360 20 75 625 3 553 3.01e-62 221
MsaG006130 MtrunA17_Chr3g0080351 26.332 638 405 17 69 682 76 672 7.14e-62 222
MsaG006130 MtrunA17_Chr5g0441581 27.700 574 373 14 69 625 32 580 1.26e-59 213
MsaG006130 MtrunA17_Chr2g0287651 26.655 574 373 15 75 625 81 629 2.58e-59 215
MsaG006130 MtrunA17_Chr8g0363841 25.111 673 447 14 60 696 8 659 1.29e-58 210
MsaG006130 MtrunA17_Chr6g0458391 27.163 578 382 12 58 625 214 762 4.26e-57 207
MsaG006130 MtrunA17_Chr6g0458391 34.400 125 76 3 69 193 91 209 3.22e-13 73.2
MsaG006130 MtrunA17_Chr1g0203051 25.490 561 374 14 75 615 85 621 2.27e-56 203
MsaG006130 MtrunA17_Chr6g0477261 30.519 462 269 15 195 625 2 442 3.19e-56 203
MsaG006130 MtrunA17_Chr2g0287061 26.445 571 351 14 69 625 183 698 2.73e-55 202
MsaG006130 MtrunA17_Chr2g0324011 25.694 576 389 13 69 625 10 565 1.02e-51 189
MsaG006130 MtrunA17_Chr2g0291581 25.963 597 387 15 69 640 71 637 2.80e-51 189
MsaG006130 MtrunA17_Chr3g0118611 30.075 399 254 5 231 623 117 496 1.28e-50 189
MsaG006130 MtrunA17_Chr1g0203721 23.917 623 427 15 71 667 65 666 9.43e-49 184
MsaG006130 MtrunA17_Chr5g0448511 29.058 382 261 4 248 623 15 392 3.61e-48 178
MsaG006130 MtrunA17_Chr1g0196541 24.458 646 432 17 75 696 3 616 4.10e-48 181
MsaG006130 MtrunA17_Chr2g0291591 23.010 578 397 14 18 577 20 567 3.20e-39 152
MsaG006130 MtrunA17_Chr1g0164591 27.864 323 214 8 149 467 1 308 1.03e-33 132
MsaG006130 MtrunA17_Chr3g0081281 49.462 93 47 0 212 304 1 93 2.35e-28 108
MsaG006130 MtrunA17_Chr7g0258861 28.328 293 197 7 69 356 10 294 3.58e-25 106
MsaG006130 MtrunA17_Chr2g0308841 25.455 220 150 4 421 630 14 229 3.72e-13 70.5
MsaG006130 MtrunA17_Chr7g0233911 31.847 157 91 7 41 195 9 151 7.06e-13 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006130 AT1G10240.2 76.017 688 146 4 8 695 6 674 0.0 1119
MsaG006130 AT1G10240.1 76.017 688 146 4 8 695 6 674 0.0 1119
MsaG006130 AT5G28530.1 42.072 637 349 7 60 681 43 674 3.16e-175 517
MsaG006130 AT5G28530.3 42.072 637 345 7 60 681 43 670 5.04e-175 516
MsaG006130 AT5G28530.2 42.072 637 345 7 60 681 43 670 5.04e-175 516
MsaG006130 AT5G28530.4 38.437 627 323 9 60 681 43 611 1.86e-140 425
MsaG006130 AT5G28530.5 38.437 627 319 9 60 681 43 607 3.43e-140 424
MsaG006130 AT4G38180.1 29.882 676 440 13 15 670 22 683 1.80e-84 283
MsaG006130 AT4G15090.1 29.355 620 401 14 69 669 51 652 2.61e-76 262
MsaG006130 AT4G15090.5 29.355 620 401 14 69 669 51 652 2.61e-76 262
MsaG006130 AT4G15090.3 29.355 620 401 14 69 669 51 652 2.61e-76 262
MsaG006130 AT4G15090.2 29.355 620 401 14 69 669 51 652 2.61e-76 262
MsaG006130 AT4G15090.4 29.355 620 401 14 69 669 51 652 2.61e-76 262
MsaG006130 AT1G76320.3 27.094 609 406 14 75 669 3 587 8.83e-61 216
MsaG006130 AT1G76320.2 26.194 607 414 13 75 669 3 587 1.17e-60 217
MsaG006130 AT1G76320.1 26.634 612 405 14 75 669 3 587 1.82e-60 216
MsaG006130 AT1G76320.4 26.634 612 405 14 75 669 3 587 1.82e-60 216
MsaG006130 AT3G22170.2 24.611 642 392 19 69 669 70 660 7.07e-57 208
MsaG006130 AT3G22170.1 24.611 642 392 19 69 669 70 660 7.07e-57 208
MsaG006130 AT2G27110.2 25.955 628 405 17 69 682 49 630 8.07e-54 199
MsaG006130 AT2G27110.1 25.955 628 405 17 69 682 49 630 8.07e-54 199
MsaG006130 AT3G06250.2 26.690 577 338 16 69 625 190 701 9.55e-52 192
MsaG006130 AT3G06250.1 26.690 577 338 16 69 625 190 701 9.55e-52 192
MsaG006130 AT3G06250.3 26.690 577 338 16 69 625 190 701 9.55e-52 192
MsaG006130 AT1G52520.1 26.361 588 365 18 69 625 85 635 6.77e-51 189
MsaG006130 AT5G18960.2 26.923 572 346 17 69 625 211 725 8.76e-50 187
MsaG006130 AT5G18960.1 26.923 572 346 17 69 625 211 725 8.76e-50 187
MsaG006130 AT4G19990.3 26.123 601 358 15 72 640 24 570 5.01e-48 181
MsaG006130 AT4G19990.4 26.123 601 358 15 72 640 24 570 5.01e-48 181
MsaG006130 AT4G19990.7 26.123 601 358 15 72 640 38 584 5.28e-48 181
MsaG006130 AT4G19990.5 26.123 601 358 15 72 640 24 570 5.57e-48 181
MsaG006130 AT4G19990.6 26.123 601 358 15 72 640 24 570 9.48e-48 181
MsaG006130 AT4G19990.2 26.123 601 358 15 72 640 24 570 9.48e-48 181
MsaG006130 AT2G27110.3 27.802 464 295 11 231 682 50 485 1.10e-46 177
MsaG006130 AT1G80010.1 27.027 592 369 17 69 625 95 658 5.57e-46 175
MsaG006130 AT2G32250.5 21.955 624 399 15 15 625 2 550 5.13e-43 166
MsaG006130 AT4G38170.1 28.045 353 246 4 280 625 45 396 1.03e-42 163
MsaG006130 AT4G19990.1 25.906 552 334 13 116 640 10 513 1.08e-42 165
MsaG006130 AT2G32250.6 21.955 624 399 15 15 625 2 550 1.30e-42 166
MsaG006130 AT2G32250.1 21.955 624 399 15 15 625 2 550 1.30e-42 166
MsaG006130 AT2G32250.3 21.955 624 399 15 15 625 2 550 1.30e-42 166
MsaG006130 AT2G32250.2 21.955 624 399 15 15 625 2 550 1.46e-42 166
MsaG006130 AT2G32250.4 21.955 624 399 15 15 625 2 550 1.46e-42 166
MsaG006130 AT2G32250.8 22.518 564 367 13 75 625 3 509 2.86e-42 164
MsaG006130 AT2G32250.7 22.518 564 367 13 75 625 3 509 2.92e-42 164

Find 154 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTGATTAACAACCTTTA+TGG 0.138871 2:-1911705 None:intergenic
AGGGAGATGGTCTGTTCTTT+TGG 0.145904 2:+1912507 MsaT006130.1:CDS
ATAAGCAAAACCACTGAATT+TGG 0.147239 2:+1911122 MsaT006130.1:CDS
CCTTCTTGGTAGGCTTTCTT+AGG 0.189142 2:+1912252 MsaT006130.1:intron
TTGACATGCCGCTGGGGATA+TGG 0.190733 2:+1911600 MsaT006130.1:CDS
TTAGAGATGAAACCATATTT+TGG 0.193583 2:-1913414 None:intergenic
GTGTACTCAAATTTAAAATT+AGG 0.227389 2:-1911461 None:intergenic
TCTTGGTTCAATGCTGTTCT+AGG 0.230975 2:+1912405 MsaT006130.1:CDS
CCACCATAATCATGAACTTT+TGG 0.234641 2:+1911178 MsaT006130.1:CDS
GTTCCAAAAGTTCATGATTA+TGG 0.235757 2:-1911181 None:intergenic
TATGGAATGCCCTGCTTCTT+TGG 0.236061 2:+1911638 MsaT006130.1:CDS
AGTTAAGCTATTCCAAAATA+TGG 0.247042 2:+1913402 MsaT006130.1:CDS
CTTAGTTTGGGTTGATGATT+TGG 0.272442 2:-1911048 None:intergenic
CTGGCACATTGTAATGAAAT+TGG 0.282052 2:-1913520 None:intergenic
ATCCTTATGTTTGCCAAAAC+AGG 0.285583 2:+1911263 MsaT006130.1:CDS
ATGAACAGAAATCCCTGTTT+TGG 0.290771 2:-1911276 None:intergenic
AAATCTAGGGTTTCTTCTTC+TGG 0.296205 2:-1911404 None:intergenic
TATTTCGTTGAGGCTTAGTT+TGG 0.297452 2:-1911061 None:intergenic
TGCACATTCTTAACAAATCT+AGG 0.298629 2:-1911418 None:intergenic
AAGAATGTGCAGAAATATTA+AGG 0.303262 2:+1911430 MsaT006130.1:CDS
AAACCAGTGACGCGCCATTC+TGG 0.316021 2:-1911149 None:intergenic
TTGGAATAGCTTAACTCTTT+CGG 0.327991 2:-1913395 None:intergenic
TAGTCATTCCCGCAAGAAAA+TGG 0.334474 2:-1912602 None:intergenic
TAGCACACAGCCAAAGAAGC+AGG 0.342849 2:-1911648 None:intergenic
TCATCAAATGATGAGACTTA+TGG 0.350578 2:+1911295 MsaT006130.1:CDS
TGATCCCTCTGACCAACATT+TGG 0.356055 2:+1910746 MsaT006130.1:CDS
AAGGAGGTCGCAGAGTTTAC+TGG 0.359371 2:+1913182 MsaT006130.1:CDS
CAAAACATGCTTTCTGCATA+TGG 0.375824 2:+1912361 MsaT006130.1:CDS
TTGGGAGGAGGAGGTGGAGG+AGG 0.380602 2:+1910765 MsaT006130.1:CDS
TCATCAAACCACACTCCATT+TGG 0.382393 2:+1910821 MsaT006130.1:CDS
ATATAGCTCGCGCTCACTTT+GGG 0.386287 2:+1912560 MsaT006130.1:CDS
AGAAAATGGCTTCTTAGATA+TGG 0.389745 2:-1912588 None:intergenic
GGAAGGAATCGAACTTGGTT+TGG 0.392049 2:-1911203 None:intergenic
TATATAGCTCGCGCTCACTT+TGG 0.393538 2:+1912559 MsaT006130.1:CDS
TGATGATGAGTTGTTGTTGT+TGG 0.396707 2:-1910849 None:intergenic
CGTAGCAGCGTGCGATTCCA+TGG 0.396995 2:-1913047 None:intergenic
ATCTTCCAGATGTGGTTGTC+AGG 0.399237 2:+1911088 MsaT006130.1:CDS
GCTTGCTCACTTTGTTGAAC+AGG 0.402511 2:+1912686 MsaT006130.1:CDS
CTTGCGGGAATGACTACAAC+TGG 0.403842 2:+1912609 MsaT006130.1:CDS
CACCGAAGATATCATACAAT+TGG 0.404756 2:-1911538 None:intergenic
GTCTAAAGAACGATTAGATA+TGG 0.411264 2:+1913453 MsaT006130.1:CDS
TAAGCAAAACCACTGAATTT+GGG 0.413718 2:+1911123 MsaT006130.1:CDS
GATCCCTCTGACCAACATTT+GGG 0.420366 2:+1910747 MsaT006130.1:CDS
GATGATGAGTTGTTGTTGTT+GGG 0.422198 2:-1910848 None:intergenic
AATAGAGAAGCCGCAGGTCT+TGG 0.423335 2:-1910937 None:intergenic
CCTCCAGAATGGCGCGTCAC+TGG 0.424652 2:+1911146 MsaT006130.1:CDS
TGGGTTGGGATAAATAATTA+TGG 0.425124 2:+1911620 MsaT006130.1:CDS
CAACATTTGGGAGGAGGAGG+TGG 0.430387 2:+1910759 MsaT006130.1:CDS
CACTGCATTTGACATGCCGC+TGG 0.431194 2:+1911592 MsaT006130.1:CDS
ATAAGCCTGACAACCACATC+TGG 0.432847 2:-1911093 None:intergenic
CTTGAGAAGTGCGTCGAACC+TGG 0.435619 2:+1911320 MsaT006130.1:CDS
CCAGAGATACTTCTACCATT+AGG 0.440893 2:+1913539 MsaT006130.1:CDS
TCTCTGAGAGTTCTTTCAAC+AGG 0.443737 2:+1913274 MsaT006130.1:CDS
CCTAATGGTAGAAGTATCTC+TGG 0.445108 2:-1913539 None:intergenic
ATTTCGTTGAGGCTTAGTTT+GGG 0.449059 2:-1911060 None:intergenic
CGGATGGGAAGATATGCATC+TGG 0.452008 2:-1913313 None:intergenic
CAAAATGTTAGCCTCAAAAC+AGG 0.452685 2:+1913019 MsaT006130.1:CDS
TGCCGCTGGGGATATGGGTT+GGG 0.453477 2:+1911606 MsaT006130.1:CDS
TGACATGCCGCTGGGGATAT+GGG 0.454706 2:+1911601 MsaT006130.1:CDS
TAGGTGAACGCTACAATGAC+TGG 0.457850 2:+1912424 MsaT006130.1:CDS
GTTTGGGTTGATGATTTGGC+AGG 0.459466 2:-1911044 None:intergenic
TAAACGGTTGTTTGCATCAA+GGG 0.461284 2:-1911483 None:intergenic
TTGTTGTTGGGTCCACCAAA+TGG 0.463735 2:-1910836 None:intergenic
TACTTTGTTTGTCACCGTGC+TGG 0.469337 2:+1911014 MsaT006130.1:CDS
TAAATTAACAATGTGTCGAT+TGG 0.471898 2:-1912538 None:intergenic
CATTTGGGAGGAGGAGGTGG+AGG 0.472758 2:+1910762 MsaT006130.1:CDS
TCAGTAAAAGGCAAATATCC+AGG 0.474275 2:-1911338 None:intergenic
ATCCAATTGTATGATATCTT+CGG 0.477575 2:+1911536 MsaT006130.1:CDS
GGAAACATTTGACCGATGTA+TGG 0.479605 2:-1910876 None:intergenic
CATGGAATCGCACGCTGCTA+CGG 0.484767 2:+1913048 MsaT006130.1:CDS
TTGACGACTTTGAACTAGGC+TGG 0.486705 2:+1912484 MsaT006130.1:CDS
TGAAGCTTCGAAAAGGCAAA+AGG 0.488243 2:-1913079 None:intergenic
GCACATTCTTAACAAATCTA+GGG 0.488962 2:-1911417 None:intergenic
AAATTCCAGAAAATTCAAAC+TGG 0.489969 2:-1913240 None:intergenic
CAGTCATCATAGATTCCTAA+TGG 0.491526 2:-1913554 None:intergenic
ACTCTTTCGGCATGATCGCT+AGG 0.495476 2:-1913382 None:intergenic
CTGAATTTGGGCCTCCAGAA+TGG 0.496915 2:+1911135 MsaT006130.1:CDS
ATCTAATCGTTCTTTAGACT+TGG 0.498595 2:-1913449 None:intergenic
TCAGTTGACGACTTTGAACT+AGG 0.498779 2:+1912480 MsaT006130.1:CDS
ACTGCATTTGACATGCCGCT+GGG 0.500632 2:+1911593 MsaT006130.1:CDS
ATGCAGGAAGGAATCGAACT+TGG 0.504851 2:-1911208 None:intergenic
ATTAACAACCTTTATGGCCC+AGG 0.508147 2:-1911699 None:intergenic
CACCTCCTCCTCCCAAATGT+TGG 0.508316 2:-1910758 None:intergenic
GCTTCAAGAACAACTTGTGT+TGG 0.510259 2:+1913096 MsaT006130.1:CDS
ATGCCGCTGGGGATATGGGT+TGG 0.515095 2:+1911605 MsaT006130.1:CDS
TTGTTCTTGAAGCTTCGAAA+AGG 0.515435 2:-1913086 None:intergenic
TAGTAGCAAAGTTTCCGTCT+TGG 0.523045 2:+1912388 MsaT006130.1:CDS
ACCGTTTAGAAAATATTGCC+TGG 0.526747 2:+1911498 MsaT006130.1:CDS
TCAAACCACACTCCATTTGG+TGG 0.527504 2:+1910824 MsaT006130.1:CDS
GAGTAAGATAATGTCTGAAG+AGG 0.527986 2:+1910662 None:intergenic
GTAGCAGCGTGCGATTCCAT+GGG 0.533610 2:-1913046 None:intergenic
CAATTAACGCCTTCATTCAA+CGG 0.534336 2:+1912643 MsaT006130.1:CDS
CTAAACGGTTGTTTGCATCA+AGG 0.535769 2:-1911484 None:intergenic
TGGAGTGTGGTTTGATGAGA+AGG 0.539574 2:-1910816 None:intergenic
GACCAACATTTGGGAGGAGG+AGG 0.539845 2:+1910756 MsaT006130.1:CDS
ACTGATACGACGCCATCGGA+TGG 0.541479 2:-1913329 None:intergenic
CTGATACGACGCCATCGGAT+GGG 0.543366 2:-1913328 None:intergenic
TCTGATATAGTTCTGTATGC+AGG 0.547078 2:-1911224 None:intergenic
TACTGCAAGATGAAACTGTC+CGG 0.547112 2:+1911669 MsaT006130.1:CDS
TGATCAGTTAATATAGTCTG+TGG 0.550040 2:-1912294 None:intergenic
TCCTTATGTTTGCCAAAACA+GGG 0.551056 2:+1911264 MsaT006130.1:CDS
CCTCCCAAATGTTGGTCAGA+GGG 0.557531 2:-1910750 None:intergenic
GAGACAGAAGAATGGAAACT+TGG 0.557670 2:-1913483 None:intergenic
TGAACTAGGCTGGAGGGAGA+TGG 0.559599 2:+1912494 MsaT006130.1:CDS
ATCATACAATTGGATGGATG+AGG 0.563319 2:-1911528 None:intergenic
CAGCTTATAATTCCTTCATG+AGG 0.566205 2:-1913208 None:intergenic
AGACATCACACAAAAGTTGA+AGG 0.566338 2:+1913163 MsaT006130.1:CDS
TGGCAGGAGTGTTGCCAGCA+CGG 0.567905 2:-1911028 None:intergenic
CCAAAAGTTCATGATTATGG+TGG 0.568510 2:-1911178 None:intergenic
GAAGATATCATACAATTGGA+TGG 0.569457 2:-1911534 None:intergenic
AGCTTATAATTCCTTCATGA+GGG 0.573229 2:-1913207 None:intergenic
TTCTGGAGGCCCAAATTCAG+TGG 0.573388 2:-1911132 None:intergenic
TCTTATGCGAGACAGAAGAA+TGG 0.582203 2:-1913491 None:intergenic
CGACTTTGAACTAGGCTGGA+GGG 0.582819 2:+1912488 MsaT006130.1:CDS
AATAGAAAATCTTCCAGATG+TGG 0.584217 2:+1911080 MsaT006130.1:CDS
GGAAAAGACGGTGTTGGCAA+AGG 0.584524 2:+1910978 MsaT006130.1:CDS
TCCTCCCAAATGTTGGTCAG+AGG 0.584559 2:-1910751 None:intergenic
ACTTAGAAACCGTTGAATGA+AGG 0.586482 2:-1912652 None:intergenic
TCTGACCAACATTTGGGAGG+AGG 0.587992 2:+1910753 MsaT006130.1:CDS
AAGTTCATGATTATGGTGGT+TGG 0.589394 2:-1911174 None:intergenic
ATGCATATCTTCCCATCCGA+TGG 0.597365 2:+1913317 MsaT006130.1:CDS
AGCACACAGCCAAAGAAGCA+GGG 0.598142 2:-1911647 None:intergenic
GTTTACTGGTCCCCTCATGA+AGG 0.604499 2:+1913196 MsaT006130.1:CDS
GATGGATGAGGCATACGACC+AGG 0.609900 2:-1911516 None:intergenic
CCAGTGACGCGCCATTCTGG+AGG 0.616513 2:-1911146 None:intergenic
TGATATCTTCGGTGATGCTG+TGG 0.618259 2:+1911547 MsaT006130.1:CDS
ACCGAAGGAAAAGACGGTGT+TGG 0.619616 2:+1910972 MsaT006130.1:CDS
TGGGTCCACCAAATGGAGTG+TGG 0.620546 2:-1910829 None:intergenic
GCACACTGATACGACGCCAT+CGG 0.621865 2:-1913333 None:intergenic
TTATGGCCCAGGAAAATGAC+CGG 0.623189 2:-1911688 None:intergenic
GGTAGCAGGTGGATCCATGT+TGG 0.624526 2:+1910683 MsaT006130.1:CDS
CTGCATTTGACATGCCGCTG+GGG 0.625737 2:+1911594 MsaT006130.1:CDS
GGATGGGAAGATATGCATCT+GGG 0.625859 2:-1913312 None:intergenic
ATTAGACGTCATCGTACCGA+AGG 0.626176 2:+1910957 MsaT006130.1:CDS
TCATAAGCAGCATCGTGAGT+GGG 0.627606 2:-1910897 None:intergenic
GGTTTCTAAGTGCACAGACA+CGG 0.627911 2:+1912664 MsaT006130.1:CDS
ATAATGTCTGAAGAGGTAGC+AGG 0.630355 2:+1910669 None:intergenic
GTCATAAGCAGCATCGTGAG+TGG 0.636238 2:-1910898 None:intergenic
TCGAAAAGGCAAAAGGAGTG+AGG 0.641424 2:-1913072 None:intergenic
GCTTATAATTCCTTCATGAG+GGG 0.641658 2:-1913206 None:intergenic
CCCTCTGACCAACATTTGGG+AGG 0.646262 2:+1910750 MsaT006130.1:CDS
ACGTCTAATAGAGAAGCCGC+AGG 0.646866 2:-1910943 None:intergenic
ACGACTTTGAACTAGGCTGG+AGG 0.648729 2:+1912487 MsaT006130.1:CDS
TAGCAGCGTGCGATTCCATG+GGG 0.649314 2:-1913045 None:intergenic
ATGTCTGAAGAGGTAGCAGG+TGG 0.649630 2:+1910672 MsaT006130.1:CDS
TGAACGCTACAATGACTGGA+AGG 0.649833 2:+1912428 MsaT006130.1:CDS
ATATAGTTCTGTATGCAGGA+AGG 0.660408 2:-1911220 None:intergenic
AGAACAGCATTGAACCAAGA+CGG 0.662125 2:-1912402 None:intergenic
CATCATCACATTCCATACAT+CGG 0.667453 2:+1910864 MsaT006130.1:CDS
CATCGTACCGAAGGAAAAGA+CGG 0.676411 2:+1910966 MsaT006130.1:CDS
GTCATCATAAACTACCAACA+TGG 0.680695 2:-1910697 None:intergenic
ATCCCAACCCATATCCCCAG+CGG 0.701870 2:-1911608 None:intergenic
CATCACACAAAAGTTGAAGG+AGG 0.714854 2:+1913166 MsaT006130.1:CDS
AGCAGCGTGCGATTCCATGG+GGG 0.736419 2:-1913044 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 1910672 1913570 1910672 ID=MsaG006130
Chr2 mRNA 1910672 1913570 1910672 ID=MsaT006130.1;Parent=MsaG006130
Chr2 exon 1910672 1911712 1910672 ID=MsaT006130.1.exon1;Parent=MsaT006130.1
Chr2 CDS 1910672 1911712 1910672 ID=cds.MsaT006130.1;Parent=MsaT006130.1
Chr2 exon 1912264 1912707 1912264 ID=MsaT006130.1.exon2;Parent=MsaT006130.1
Chr2 CDS 1912264 1912707 1912264 ID=cds.MsaT006130.1;Parent=MsaT006130.1
Chr2 exon 1912956 1913570 1912956 ID=MsaT006130.1.exon3;Parent=MsaT006130.1
Chr2 CDS 1912956 1913570 1912956 ID=cds.MsaT006130.1;Parent=MsaT006130.1
Gene Sequence

>MsaT006130.1

ATGTCTGAAGAGGTAGCAGGTGGATCCATGTTGGTAGTTTATGATGACGATGATATTGATGTTGATGTTGACGATGATCCCTCTGACCAACATTTGGGAGGAGGAGGTGGAGGAGGATGCGATACAAGCAGCACAGAAGACTCTCCTTCTCATCAAACCACACTCCATTTGGTGGACCCAACAACAACAACTCATCATCACATTCCATACATCGGTCAAATGTTTCCCACTCACGATGCTGCTTATGACTTCTATACTCATTTTGCCAAGACCTGCGGCTTCTCTATTAGACGTCATCGTACCGAAGGAAAAGACGGTGTTGGCAAAGGACTTACTAGACGCTACTTTGTTTGTCACCGTGCTGGCAACACTCCTGCCAAATCATCAACCCAAACTAAGCCTCAACGAAATAGAAAATCTTCCAGATGTGGTTGTCAGGCTTATATGCGCATAAGCAAAACCACTGAATTTGGGCCTCCAGAATGGCGCGTCACTGGTTTTGCCAACCACCATAATCATGAACTTTTGGAACCAAACCAAGTTCGATTCCTTCCTGCATACAGAACTATATCAGATGCTGACAAAAATCGGATCCTTATGTTTGCCAAAACAGGGATTTCTGTTCATCAAATGATGAGACTTATGGAGCTTGAGAAGTGCGTCGAACCTGGATATTTGCCTTTTACTGAAAAGGACGTAAGGAATTTACTCCAGTCATTTAGAAAATTAGATCCAGAAGAAGAAACCCTAGATTTGTTAAGAATGTGCAGAAATATTAAGGACAAAGATCCTAATTTTAAATTTGAGTACACCCTTGATGCAAACAACCGTTTAGAAAATATTGCCTGGTCGTATGCCTCATCCATCCAATTGTATGATATCTTCGGTGATGCTGTGGTGTTTGATACAACACATCGTCTCACTGCATTTGACATGCCGCTGGGGATATGGGTTGGGATAAATAATTATGGAATGCCCTGCTTCTTTGGCTGTGTGCTACTGCAAGATGAAACTGTCCGGTCATTTTCCTGGGCCATAAAGGCTTTCTTAGGTTTTATGAATGGGAAGGCTCCACAGACTATATTAACTGATCAAAATATTTGTCTCAAAGAAGCACTATCTGCAGAAATGCTGATGACAAAACATGCTTTCTGCATATGGATGATAGTAGCAAAGTTTCCGTCTTGGTTCAATGCTGTTCTAGGTGAACGCTACAATGACTGGAAGGCTGAGTTTTATAGACTTTATAATCTTGAATCAGTTGACGACTTTGAACTAGGCTGGAGGGAGATGGTCTGTTCTTTTGGGCTGCATTCCAATCGACACATTGTTAATTTATATAGCTCGCGCTCACTTTGGGCATTGCCATATCTAAGAAGCCATTTTCTTGCGGGAATGACTACAACTGGTCAGTCAAAGTCAATTAACGCCTTCATTCAACGGTTTCTAAGTGCACAGACACGGCTTGCTCACTTTGTTGAACAGGCAGCTGTTGCTGTTGATTTTAAAGATCAAACTGGTGAACAACAAACCATGCAGCAGAATCTCCAAAATGTTAGCCTCAAAACAGGAGCCCCCATGGAATCGCACGCTGCTACGGTCCTCACTCCTTTTGCCTTTTCGAAGCTTCAAGAACAACTTGTGTTGGCTGCACATTATGCATCTTTTTCAGTTGAAGATGGTTTTCTTGTGAGACATCACACAAAAGTTGAAGGAGGTCGCAGAGTTTACTGGTCCCCTCATGAAGGAATTATAAGCTGCAGCTGTCACCAGTTTGAATTTTCTGGAATTTTGTGTAGGCACTCTCTGAGAGTTCTTTCAACAGGAAATTGCTTTCAAATCCCAGATGCATATCTTCCCATCCGATGGCGTCGTATCAGTGTGCCTTCTTCTAAGATTCTTCAAAATGCACCTAGCGATCATGCCGAAAGAGTTAAGCTATTCCAAAATATGGTTTCATCTCTAATTACAGAATCTGCCAAGTCTAAAGAACGATTAGATATGGCAACAGACCAAGTTTCCATTCTTCTGTCTCGCATAAGAGAGCAACCAATTTCATTACAATGTGCCAGAGATACTTCTACCATTAGGAATCTATGA
Protein sequence

>MsaT006130.1

MSEEVAGGSMLVVYDDDDIDVDVDDDPSDQHLGGGGGGGCDTSSTEDSPSHQTTLHLVDPTTTTHHHIPYIGQMFPTHDAAYDFYTHFAKTCGFSIRRHRTEGKDGVGKGLTRRYFVCHRAGNTPAKSSTQTKPQRNRKSSRCGCQAYMRISKTTEFGPPEWRVTGFANHHNHELLEPNQVRFLPAYRTISDADKNRILMFAKTGISVHQMMRLMELEKCVEPGYLPFTEKDVRNLLQSFRKLDPEEETLDLLRMCRNIKDKDPNFKFEYTLDANNRLENIAWSYASSIQLYDIFGDAVVFDTTHRLTAFDMPLGIWVGINNYGMPCFFGCVLLQDETVRSFSWAIKAFLGFMNGKAPQTILTDQNICLKEALSAEMLMTKHAFCIWMIVAKFPSWFNAVLGERYNDWKAEFYRLYNLESVDDFELGWREMVCSFGLHSNRHIVNLYSSRSLWALPYLRSHFLAGMTTTGQSKSINAFIQRFLSAQTRLAHFVEQAAVAVDFKDQTGEQQTMQQNLQNVSLKTGAPMESHAATVLTPFAFSKLQEQLVLAAHYASFSVEDGFLVRHHTKVEGGRRVYWSPHEGIISCSCHQFEFSGILCRHSLRVLSTGNCFQIPDAYLPIRWRRISVPSSKILQNAPSDHAERVKLFQNMVSSLITESAKSKERLDMATDQVSILLSRIREQPISLQCARDTSTIRNL*