AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006351


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006351 MtrunA17_Chr2g0280161 95.467 353 16 0 1 353 1 353 0.0 700
MsaG006351 MtrunA17_Chr4g0049331 55.587 349 111 10 7 353 4 310 2.76e-115 337
MsaG006351 MtrunA17_Chr4g0054561 40.230 348 172 14 5 345 2 320 2.27e-59 194
MsaG006351 MtrunA17_Chr4g0054551 40.230 348 172 14 5 345 2 320 2.45e-58 191
MsaG006351 MtrunA17_Chr5g0400661 44.358 257 115 6 106 346 86 330 2.17e-57 189
MsaG006351 MtrunA17_Chr5g0400621 52.632 190 87 2 166 353 144 332 5.00e-55 183
MsaG006351 MtrunA17_Chr4g0054581 44.643 224 107 5 138 353 163 377 2.53e-49 169
MsaG006351 MtrunA17_Chr5g0400671 34.104 346 200 10 5 348 2 321 4.37e-49 167
MsaG006351 MtrunA17_Chr6g0468491 46.667 195 99 4 161 353 121 312 2.17e-48 165
MsaG006351 MtrunA17_Chr8g0336681 42.081 221 116 4 134 346 115 331 2.42e-48 166
MsaG006351 MtrunA17_Chr4g0054571 54.749 179 69 4 177 346 150 325 4.19e-48 165
MsaG006351 MtrunA17_Chr2g0333621 50.847 177 81 2 173 346 134 307 2.13e-46 160
MsaG006351 MtrunA17_Chr2g0307131 43.842 203 107 3 151 353 159 354 4.35e-46 160
MsaG006351 MtrunA17_Chr2g0307141 46.524 187 83 4 175 353 181 358 9.00e-45 157
MsaG006351 MtrunA17_Chr7g0225901 45.304 181 95 2 151 331 65 241 3.30e-43 150
MsaG006351 MtrunA17_Chr4g0033391 43.548 186 99 2 168 353 140 319 4.78e-43 152
MsaG006351 MtrunA17_Chr4g0054621 39.904 208 107 3 156 353 141 340 1.25e-41 148
MsaG006351 MtrunA17_Chr4g0002801 45.161 186 93 3 168 346 143 326 1.36e-39 142
MsaG006351 MtrunA17_Chr4g0002781 46.067 178 90 2 173 346 170 345 1.45e-39 143
MsaG006351 MtrunA17_Chr2g0333641 46.927 179 92 2 173 351 133 308 1.64e-39 142
MsaG006351 MtrunA17_Chr4g0033291 41.627 209 111 3 151 352 122 326 4.92e-39 141
MsaG006351 MtrunA17_Chr2g0333611 42.697 178 89 5 174 346 42 211 6.91e-39 137
MsaG006351 MtrunA17_Chr5g0400641 46.452 155 82 1 180 334 1 154 1.48e-38 135
MsaG006351 MtrunA17_Chr4g0033281 40.952 210 112 3 151 353 120 324 1.23e-37 137
MsaG006351 MtrunA17_Chr2g0333541 43.094 181 100 2 168 346 85 264 5.96e-36 132
MsaG006351 MtrunA17_Chr6g0468461 41.765 170 84 2 161 330 157 311 8.73e-33 125
MsaG006351 MtrunA17_Chr7g0249811 41.860 172 90 1 175 346 163 324 4.16e-31 120
MsaG006351 MtrunA17_Chr7g0233441 38.816 152 82 2 200 351 57 197 1.06e-25 102
MsaG006351 MtrunA17_Chr4g0002831 35.260 173 99 4 180 351 45 205 3.20e-24 98.6
MsaG006351 MtrunA17_Chr4g0002851 37.500 152 76 2 200 351 65 197 1.62e-23 96.3
MsaG006351 MtrunA17_Chr1g0185621 34.395 157 93 3 177 324 475 630 5.75e-20 91.3
MsaG006351 MtrunA17_Chr7g0275821 31.618 136 84 2 177 312 252 378 2.56e-16 79.7
MsaG006351 MtrunA17_Chr8g0366751 33.143 175 106 3 150 313 434 608 1.16e-15 78.2
MsaG006351 MtrunA17_Chr5g0411341 31.333 150 87 3 177 313 493 639 3.78e-15 76.6
MsaG006351 MtrunA17_Chr8g0346161 32.168 143 85 3 177 313 291 427 8.60e-15 75.1
MsaG006351 MtrunA17_Chr3g0131691 28.846 156 102 2 178 324 299 454 1.00e-14 75.1
MsaG006351 MtrunA17_Chr7g0260841 54.386 57 26 0 177 233 355 411 6.43e-14 72.8
MsaG006351 MtrunA17_Chr8g0346151 27.711 166 99 3 177 330 279 435 1.31e-13 71.6
MsaG006351 MtrunA17_Chr7g0252041 28.302 159 99 3 179 324 288 444 2.36e-13 70.9
MsaG006351 MtrunA17_Chr8g0365711 25.843 178 110 5 166 327 227 398 1.04e-11 65.9
MsaG006351 MtrunA17_Chr1g0211081 38.710 93 53 2 168 257 309 400 4.86e-11 63.9
MsaG006351 MtrunA17_Chr8g0386921 25.962 208 121 5 174 352 403 606 5.00e-11 63.9
MsaG006351 MtrunA17_Chr2g0298751 49.091 55 28 0 178 232 53 107 9.78e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006351 AT4G37850.2 56.757 185 72 4 173 353 148 328 1.01e-59 196
MsaG006351 AT4G37850.1 56.757 185 72 4 173 353 148 328 1.01e-59 196
MsaG006351 AT2G22760.1 49.153 177 87 3 175 348 117 293 1.14e-48 166
MsaG006351 AT2G22770.1 46.809 188 86 2 177 350 132 319 6.35e-45 157
MsaG006351 AT2G22750.2 50.811 185 84 3 173 352 122 304 3.16e-43 152
MsaG006351 AT2G22750.3 50.811 185 84 3 173 352 150 332 5.94e-43 152
MsaG006351 AT2G22750.5 50.811 185 84 3 173 352 146 328 6.40e-43 152
MsaG006351 AT2G22750.1 50.811 185 83 4 173 352 122 303 6.42e-42 148
MsaG006351 AT2G22760.2 47.468 158 81 2 175 330 117 274 1.26e-41 147
MsaG006351 AT2G22750.4 50.811 185 83 4 173 352 150 331 1.36e-41 148
MsaG006351 AT1G32640.1 36.025 161 87 4 165 313 432 588 1.21e-18 87.8
MsaG006351 AT5G46760.1 34.783 161 101 2 177 334 415 574 3.47e-17 83.2
MsaG006351 AT2G46510.1 59.649 57 23 0 177 233 395 451 1.13e-16 81.6
MsaG006351 AT4G16430.1 37.143 140 70 5 118 241 247 384 3.08e-16 80.1
MsaG006351 AT1G01260.1 52.174 69 32 1 168 235 423 491 8.13e-16 79.0
MsaG006351 AT1G01260.2 52.174 69 32 1 168 235 423 491 8.13e-16 79.0
MsaG006351 AT1G01260.3 52.174 69 32 1 168 235 423 491 8.13e-16 79.0
MsaG006351 AT4G17880.1 31.013 158 107 2 177 334 416 571 1.30e-15 78.6
MsaG006351 AT1G12860.2 30.921 152 100 3 178 324 268 419 2.11e-14 74.3
MsaG006351 AT1G12860.1 31.126 151 99 3 179 324 269 419 2.66e-14 74.3
MsaG006351 AT3G26744.6 30.128 156 100 3 178 324 308 463 2.75e-13 71.2
MsaG006351 AT3G26744.1 30.128 156 100 3 178 324 308 463 3.06e-13 70.9
MsaG006351 AT3G26744.4 30.128 156 100 3 178 324 308 463 3.06e-13 70.9
MsaG006351 AT3G26744.2 30.128 156 100 3 178 324 308 463 3.06e-13 70.9
MsaG006351 AT5G57150.3 30.000 170 97 5 173 327 51 213 1.07e-12 67.0
MsaG006351 AT5G57150.5 30.000 170 97 5 173 327 50 212 1.19e-12 67.0
MsaG006351 AT5G57150.2 30.000 170 97 5 173 327 51 213 1.32e-12 67.0
MsaG006351 AT4G09820.1 31.343 134 65 3 178 308 364 473 1.44e-12 68.9
MsaG006351 AT5G57150.4 30.000 170 97 5 173 327 51 213 1.63e-12 67.4
MsaG006351 AT2G16910.1 27.879 165 90 4 178 317 315 475 1.90e-12 68.9
MsaG006351 AT5G41315.3 28.824 170 99 3 160 307 429 598 2.05e-12 68.6
MsaG006351 AT5G41315.1 28.824 170 99 3 160 307 424 593 2.09e-12 68.6
MsaG006351 AT5G41315.2 28.824 170 99 3 160 307 432 601 2.11e-12 68.6
MsaG006351 AT5G57150.1 30.000 170 97 5 173 327 50 212 2.32e-12 66.6
MsaG006351 AT3G61950.6 30.496 141 87 3 177 311 128 263 4.55e-12 66.2
MsaG006351 AT4G00870.1 46.875 64 34 0 178 241 250 313 6.14e-12 67.0
MsaG006351 AT3G61950.3 30.496 141 87 3 177 311 179 314 8.56e-12 65.9
MsaG006351 AT3G61950.2 30.496 141 87 3 177 311 128 263 9.00e-12 65.5
MsaG006351 AT3G61950.1 30.496 141 87 3 177 311 179 314 1.66e-11 65.1
MsaG006351 AT1G63650.3 27.564 156 96 3 169 307 397 552 1.86e-11 65.9
MsaG006351 AT1G63650.1 27.564 156 96 3 169 307 397 552 1.86e-11 65.9
MsaG006351 AT1G63650.2 27.564 156 96 3 169 307 397 552 1.86e-11 65.9
MsaG006351 AT4G29930.6 27.778 180 107 6 178 349 55 219 1.97e-11 63.5
MsaG006351 AT4G29930.2 28.161 174 111 4 178 349 55 216 3.16e-11 62.8
MsaG006351 AT4G29930.1 25.773 194 119 3 178 349 55 245 5.51e-11 62.8

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAATCTTGCTTCAATTTC+AGG 0.082646 2:-4553076 None:intergenic
AATAAATATCTTACCATTTC+AGG 0.175967 2:-4551561 None:intergenic
AACTTAACTGCTTCTGCTTT+TGG 0.208058 2:+4551868 MsaT006351.1:CDS
AGCAGAAGCAGTTAAGTTAT+TGG 0.221213 2:-4551863 None:intergenic
ATGGACAAAGCTTCTGTTCT+TGG 0.230457 2:+4552881 MsaT006351.1:CDS
AATAGCAGTTCCTTGACTTT+TGG 0.241912 2:+4553196 MsaT006351.1:CDS
TTTCACCGGTCTTTCGTTTC+CGG 0.252447 2:-4551965 None:intergenic
GTTGTTGAAGTTTGGATTAT+TGG 0.283921 2:-4551917 None:intergenic
ATCACTCAAGGTAACTTGTT+TGG 0.298879 2:+4552180 MsaT006351.1:CDS
TTTGATACCTTCTTTAGGTT+TGG 0.325711 2:-4552354 None:intergenic
CATTATTTGATACCTTCTTT+AGG 0.327577 2:-4552359 None:intergenic
ATGAACTCTGCAGGGGATAA+TGG 0.331301 2:+4551789 MsaT006351.1:intron
TCACTCAAGGTAACTTGTTT+GGG 0.334384 2:+4552181 MsaT006351.1:CDS
CTGTTCTTGGAGAAGCTATA+AGG 0.338206 2:+4552894 MsaT006351.1:CDS
TATCAGAGGGTTACCTGAAA+TGG 0.381568 2:+4551548 MsaT006351.1:CDS
TTTCAGGATGAGTTCTCATA+TGG 0.392590 2:+4551515 None:intergenic
GAAAATTACCTGAGCAATGA+TGG 0.451657 2:-4553239 None:intergenic
TTGAATTACACAAATCAGTT+GGG 0.457187 2:+4552087 MsaT006351.1:CDS
TCAAACAATGTAGTCTTATG+AGG 0.459967 2:-4553111 None:intergenic
TATAAGGTACTTAAAGCAAA+TGG 0.467859 2:+4552910 MsaT006351.1:CDS
GAGCAATGATGGTTATATCA+AGG 0.480152 2:-4553228 None:intergenic
TTTGAATTACACAAATCAGT+TGG 0.490344 2:+4552086 MsaT006351.1:CDS
TATTGGTATCAATAGAGCTT+AGG 0.492237 2:-4551846 None:intergenic
TTATTTCTTAACTGTTTCAC+CGG 0.499185 2:-4551979 None:intergenic
AACAGTTAAGAAATAACAGC+TGG 0.500916 2:+4551986 MsaT006351.1:CDS
TGATATAACCATCATTGCTC+AGG 0.502657 2:+4553231 MsaT006351.1:CDS
GGACACACCATCTCAGACTT+AGG 0.503073 2:-4552120 None:intergenic
AGAGAAAGTGAGTGTTCTTG+AGG 0.506732 2:+4552934 MsaT006351.1:CDS
CTTGAACAGCTTTAAAGACA+TGG 0.514024 2:-4552212 None:intergenic
TCATATTGTGTATCTGATGT+TGG 0.522543 2:-4552027 None:intergenic
AGCTTAGGTGATTATTGAAG+TGG 0.522860 2:-4551831 None:intergenic
GGTCTTTCGTTTCCGGTAGA+CGG 0.530949 2:-4551958 None:intergenic
TGGCAATTTGCAGATGGATG+TGG 0.531836 2:+4553620 MsaT006351.1:intron
TGCATGGAGACTCCGTCTAC+CGG 0.552215 2:+4551946 MsaT006351.1:CDS
TCTTGTTCCAAACCTAAAGA+AGG 0.556354 2:+4552347 MsaT006351.1:CDS
CTACATTGTTTGAAGAGTCA+AGG 0.557167 2:+4553121 MsaT006351.1:CDS
TGTCTTTAAAGCTGTTCAAG+AGG 0.569037 2:+4552215 MsaT006351.1:CDS
TGTGTAATTCAAATCAGCAA+AGG 0.572771 2:-4552076 None:intergenic
GCAAACATGTTGATCACTCA+AGG 0.574022 2:+4552168 MsaT006351.1:CDS
AGCAATGATGGTTATATCAA+GGG 0.578216 2:-4553227 None:intergenic
CTTCAACAACAAAACATGCA+TGG 0.578541 2:+4551930 MsaT006351.1:CDS
CAGACAGAGCGATGAATCGT+TGG 0.578725 2:-4552323 None:intergenic
TGGAGATTTCATCTATCAGA+GGG 0.587975 2:+4551535 MsaT006351.1:CDS
GAAAGTGAGTGTTCTTGAGG+AGG 0.588414 2:+4552937 MsaT006351.1:CDS
ATTGAAGCCTAAGTCTGAGA+TGG 0.590244 2:+4552113 MsaT006351.1:CDS
ATGGAGATTTCATCTATCAG+AGG 0.606668 2:+4551534 MsaT006351.1:CDS
GGTGAAATTCCCAAAAGTCA+AGG 0.621239 2:-4553206 None:intergenic
TATTGTGTATCTGATGTTGG+AGG 0.636554 2:-4552024 None:intergenic
GTCTACCGGAAACGAAAGAC+CGG 0.653717 2:+4551960 MsaT006351.1:CDS
AACTCTGCAGGGGATAATGG+AGG 0.663168 2:+4551792 MsaT006351.1:intron
ATGATCACATCGTAGCGGAG+AGG 0.690331 2:+4552280 MsaT006351.1:CDS
TGACAGTGAAGGATCTTGTG+AGG 0.718300 2:+4553652 MsaT006351.1:CDS
AGCTCATGATCACATCGTAG+CGG 0.723921 2:+4552275 MsaT006351.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 4551522 4553707 4551522 ID=MsaG006351
Chr2 mRNA 4551522 4553707 4551522 ID=MsaT006351.1;Parent=MsaG006351
Chr2 exon 4551522 4551569 4551522 ID=MsaT006351.1.exon1;Parent=MsaT006351.1
Chr2 CDS 4551522 4551569 4551522 ID=cds.MsaT006351.1;Parent=MsaT006351.1
Chr2 exon 4551802 4552368 4551802 ID=MsaT006351.1.exon2;Parent=MsaT006351.1
Chr2 CDS 4551802 4552368 4551802 ID=cds.MsaT006351.1;Parent=MsaT006351.1
Chr2 exon 4552881 4553252 4552881 ID=MsaT006351.1.exon3;Parent=MsaT006351.1
Chr2 CDS 4552881 4553252 4552881 ID=cds.MsaT006351.1;Parent=MsaT006351.1
Chr2 exon 4553633 4553707 4553633 ID=MsaT006351.1.exon4;Parent=MsaT006351.1
Chr2 CDS 4553633 4553707 4553633 ID=cds.MsaT006351.1;Parent=MsaT006351.1
Gene Sequence

>MsaT006351.1

ATGAGTTCTCATATGGAGATTTCATCTATCAGAGGGTTACCTGAAATGGGGATAATGGAGGATCCTAATTTTTTACACCACTTCAATAATCACCTAAGCTCTATTGATACCAATAACTTAACTGCTTCTGCTTTTGGAGATGCTTTACAAAAACATATTTTGTCCAATAATCCAAACTTCAACAACAAAACATGCATGGAGACTCCGTCTACCGGAAACGAAAGACCGGTGAAACAGTTAAGAAATAACAGCTGGAATTACAACAATAGTCCTCCAACATCAGATACACAATATGATAATTGTTGCTCTAATAATCTTCTTTCCTTTGCTGATTTGAATTACACAAATCAGTTGGGACTATTGAAGCCTAAGTCTGAGATGGTGTGTCCGAAAATCGATAACACTAGTACTCTTGCAAACATGTTGATCACTCAAGGTAACTTGTTTGGGAATCAAAACCATGTCTTTAAAGCTGTTCAAGAGGCTAAAGATATTGAGAATCGTCCTAATAAAATCTCTCAAGCTCATGATCACATCGTAGCGGAGAGGAAGCGTCGCGAGAAGCTTAGCCAACGATTCATCGCTCTGTCTGCTCTTGTTCCAAACCTAAAGAAGATGGACAAAGCTTCTGTTCTTGGAGAAGCTATAAGGTACTTAAAGCAAATGGAAGAGAAAGTGAGTGTTCTTGAGGAGGAACAAAAAAGGAAGAAAACTGTGGAATCTGTTGTAATAGTGAAGAAATCTCAACTTTCTATTAATGATGCTGAAAACTCTTGTTCAGACACTGATAGTACATTTGATGAGACATTACCTGAAATTGAAGCAAGATTTTGTGAAAGAAGTGTCCTCATAAGACTACATTGTTTGAAGAGTCAAGGAGTTATTGAGAAAATTATGAGTGAAATTGAGAAACTTCATCTGAAAGTCATCAATAGCAGTTCCTTGACTTTTGGGAATTTCACCCTTGATATAACCATCATTGCTCAGATGGATGTGGAATTTTGCATGACAGTGAAGGATCTTGTGAGGAAGCTACGTTCAGCATATTCATCTTTCATGTGA

Protein sequence

>MsaT006351.1

MSSHMEISSIRGLPEMGIMEDPNFLHHFNNHLSSIDTNNLTASAFGDALQKHILSNNPNFNNKTCMETPSTGNERPVKQLRNNSWNYNNSPPTSDTQYDNCCSNNLLSFADLNYTNQLGLLKPKSEMVCPKIDNTSTLANMLITQGNLFGNQNHVFKAVQEAKDIENRPNKISQAHDHIVAERKRREKLSQRFIALSALVPNLKKMDKASVLGEAIRYLKQMEEKVSVLEEEQKRKKTVESVVIVKKSQLSINDAENSCSDTDSTFDETLPEIEARFCERSVLIRLHCLKSQGVIEKIMSEIEKLHLKVINSSSLTFGNFTLDITIIAQMDVEFCMTVKDLVRKLRSAYSSFM*