AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006377


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006377 MtrunA17_Chr2g0280431 89.647 425 34 4 1 421 1 419 0.0 665
MsaG006377 MtrunA17_Chr4g0061741 54.430 158 65 4 235 390 114 266 1.07e-39 143
MsaG006377 MtrunA17_Chr3g0113131 35.793 271 142 8 155 407 106 362 7.41e-33 127
MsaG006377 MtrunA17_Chr7g0273821 30.921 152 83 4 153 293 68 208 1.12e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006377 AT1G61730.1 32.333 433 208 16 9 419 3 372 4.74e-28 114
MsaG006377 AT4G00390.1 31.034 406 212 14 16 409 6 355 3.94e-22 97.4
MsaG006377 AT1G11510.1 30.295 373 188 15 59 407 15 339 3.25e-20 91.7
MsaG006377 AT1G11510.2 29.477 363 175 13 59 407 15 310 6.41e-18 84.7
MsaG006377 AT4G25210.1 26.567 335 182 11 111 414 63 364 1.12e-16 81.6
MsaG006377 AT1G66420.1 36.090 133 69 4 161 289 53 173 2.51e-14 73.6
MsaG006377 AT2G01370.1 31.214 173 95 5 130 288 7 169 2.00e-13 70.5
MsaG006377 AT1G44810.1 26.923 286 126 9 8 289 3 209 2.17e-13 70.9
MsaG006377 AT4G01260.1 29.433 282 146 9 158 411 57 313 3.56e-12 67.4
MsaG006377 AT2G25650.1 35.714 140 72 6 208 336 67 199 1.91e-11 65.9
MsaG006377 AT5G14280.1 28.936 235 134 8 208 412 63 294 9.20e-11 63.2

Find 114 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGGGCTTGGGCTTGGACT+TGG 0.123490 2:-4814741 None:intergenic
CTTGGGCTTGGACTTGGGTT+TGG 0.137721 2:-4814735 None:intergenic
GAAGCAAGTGGTTTAACTTT+AGG 0.199493 2:-4814697 None:intergenic
ATTGGGCTTGGGCTTGGGCT+TGG 0.201008 2:-4814747 None:intergenic
TTGGGCTTGGACTTGGGTTT+GGG 0.211588 2:-4814734 None:intergenic
GATTCAGATTCAGCGGGTTT+AGG 0.217235 2:-4814592 None:intergenic
AAGCAAGTGGTTTAACTTTA+GGG 0.240022 2:-4814696 None:intergenic
CAAGAAGAAATCAAGCAAAT+AGG 0.257807 2:+4815566 MsaT006377.1:CDS
GAGTTGATTGGGCTTGGGCT+TGG 0.262442 2:-4814753 None:intergenic
TTGGGCTTGGGCTTGGACTT+GGG 0.274782 2:-4814740 None:intergenic
GCACCGGAGTTGATTGGGCT+TGG 0.275880 2:-4814759 None:intergenic
AGTTGATTGGGCTTGGGCTT+GGG 0.282116 2:-4814752 None:intergenic
ATTCAGATTCAGCGGGTTTA+GGG 0.293343 2:-4814591 None:intergenic
TGAATCGCGCTGAGGTGGTT+AGG 0.312947 2:+4815514 MsaT006377.1:CDS
CACCGGAGTTGATTGGGCTT+GGG 0.331565 2:-4814758 None:intergenic
TTCTTCGTTGACTACTTTGT+CGG 0.338007 2:-4814422 None:intergenic
GTGGGTTCCGATTTCTGTTC+CGG 0.351302 2:-4814661 None:intergenic
TTGTGATTCTCAAAGGGCTT+GGG 0.351413 2:+4814965 MsaT006377.1:CDS
GGCACGCTTAGTTCCGGACT+TGG 0.351838 2:-4814785 None:intergenic
GTCAGATAGGGTTGAGTTGG+AGG 0.367989 2:+4815449 MsaT006377.1:CDS
TGCTCTCTTATCATGAGCTT+TGG 0.370081 2:-4815142 None:intergenic
GCAGACTAGATTGGCATTGC+AGG 0.370825 2:+4815539 MsaT006377.1:CDS
TCAGAATCCGGTTCTGAATC+TGG 0.376206 2:+4814619 MsaT006377.1:CDS
AAGAAGAAATCAAGCAAATA+GGG 0.379593 2:+4815567 MsaT006377.1:CDS
AGTGGTTTAACTTTAGGGTT+AGG 0.384389 2:-4814691 None:intergenic
TTCGGCGGCACGCTTAGTTC+CGG 0.394238 2:-4814791 None:intergenic
TGGTTGGCACCGGAGTTGAT+TGG 0.408980 2:-4814765 None:intergenic
TTCTGAACCGGAACAGAAAT+CGG 0.410111 2:+4814654 MsaT006377.1:CDS
GCGGGTTTAGGGGTAGAGAT+AGG 0.413172 2:-4814580 None:intergenic
CTTGTGATTCTCAAAGGGCT+TGG 0.413788 2:+4814964 MsaT006377.1:CDS
GGTTGGCACCGGAGTTGATT+GGG 0.414661 2:-4814764 None:intergenic
TCTCTTCAATTGCTGAGCAT+TGG 0.418880 2:-4815073 None:intergenic
GAATCGCGCTGAGGTGGTTA+GGG 0.419146 2:+4815515 MsaT006377.1:CDS
CTCGTCGGAGGAAGAGGCGG+TGG 0.434145 2:-4814371 None:intergenic
GGGGTAGAGATAGGAGTAGA+AGG 0.435130 2:-4814571 None:intergenic
TCTGAATCCGGCTCAGAATC+CGG 0.447335 2:+4814607 MsaT006377.1:CDS
ATCGTTTCCAGTGTTACTTT+CGG 0.449680 2:-4814809 None:intergenic
CGCTTAGTTCCGGACTTGGT+TGG 0.450754 2:-4814781 None:intergenic
TCGGAGGAAGAGGCGGTGGG+TGG 0.451878 2:-4814367 None:intergenic
TAAGAATGAAAGTGGAAAAG+TGG 0.453012 2:+4815311 MsaT006377.1:CDS
TTCGGTTCCAGATTCAGAAC+CGG 0.454128 2:-4814626 None:intergenic
AACCGAATCTGATTCTGAAC+CGG 0.455116 2:+4814642 MsaT006377.1:CDS
TCCATCTCCTTCTTCTTCTG+AGG 0.458431 2:-4814458 None:intergenic
GGAGAGGTGGAAGAAGTTGC+AGG 0.459564 2:+4815470 MsaT006377.1:CDS
GGTTTAACTTTAGGGTTAGG+AGG 0.459566 2:-4814688 None:intergenic
TTCCGGTTCAGAATCAGATT+CGG 0.461387 2:-4814644 None:intergenic
AAGGAAGCAGCTGCATTTCA+CGG 0.465241 2:+4815021 MsaT006377.1:CDS
AATCACAAAGAGTGCTAAGA+AGG 0.466107 2:+4815236 MsaT006377.1:CDS
TTGATCCTCGTCGGAGGAAG+AGG 0.466920 2:-4814377 None:intergenic
ACTCCGGTGCCAACCAAGTC+CGG 0.469333 2:+4814772 MsaT006377.1:CDS
ATAGTTACTAAGAATGAAAG+TGG 0.473928 2:+4815303 MsaT006377.1:CDS
GGAGTCAGATAGGGTTGAGT+TGG 0.474759 2:+4815446 MsaT006377.1:CDS
TGTGATTCTCAAAGGGCTTG+GGG 0.482044 2:+4814966 MsaT006377.1:CDS
GTTCCGGACTTGGTTGGCAC+CGG 0.486396 2:-4814775 None:intergenic
TTAACTTTAGGGTTAGGAGG+AGG 0.489961 2:-4814685 None:intergenic
GGGTATAGCTGTGCTGGATA+AGG 0.490129 2:+4815392 MsaT006377.1:CDS
GGGTTGAGTTGGAGGAGAGG+TGG 0.490174 2:+4815457 MsaT006377.1:CDS
GAGATAGGAGTAGAAGGAGG+AGG 0.494629 2:-4814565 None:intergenic
TTCAGAACCGGATTCTGAGC+CGG 0.501712 2:-4814614 None:intergenic
ACGAGCTTGTGATTCTCAAA+GGG 0.508640 2:+4814959 MsaT006377.1:CDS
TCTAAGGAAGCTGAAGAAAA+CGG 0.508657 2:+4815198 MsaT006377.1:CDS
AAGAAAACCACCGGTGGTGG+TGG 0.509946 2:+4814847 MsaT006377.1:CDS
AAATGGTGGTTCTGCTAGGA+AGG 0.513798 2:+4815272 MsaT006377.1:CDS
AAGAAGAAAACCACCGGTGG+TGG 0.515031 2:+4814844 MsaT006377.1:CDS
GAAGTTGCAGGCTGCCGAGA+TGG 0.515486 2:+4815482 MsaT006377.1:CDS
ATAGGGTTGAGTTGGAGGAG+AGG 0.516403 2:+4815454 MsaT006377.1:CDS
GGTTAGGGAGCAGACTAGAT+TGG 0.517395 2:+4815530 MsaT006377.1:CDS
AGATAGGAGTAGAAGGAGGA+GGG 0.520024 2:-4814564 None:intergenic
GAAGGAGATGGAGAAGAAGA+AGG 0.521213 2:+4814469 MsaT006377.1:CDS
GACGAGCTTGTGATTCTCAA+AGG 0.523580 2:+4814958 MsaT006377.1:CDS
AAACCCGCTGAATCTGAATC+CGG 0.523805 2:+4814595 MsaT006377.1:CDS
CGGAGGAAGAGGCGGTGGGT+GGG 0.524036 2:-4814366 None:intergenic
TCGTTGACTACTTTGTCGGC+TGG 0.525334 2:-4814418 None:intergenic
GTTAAATTTGATGAGGCTGC+GGG 0.528643 2:+4815372 MsaT006377.1:CDS
TGCTCAGCAATTGAAGAGAA+AGG 0.529680 2:+4815077 MsaT006377.1:CDS
GGCTGCGGGTATAGCTGTGC+TGG 0.534970 2:+4815386 MsaT006377.1:CDS
GGTTAAATTTGATGAGGCTG+CGG 0.538193 2:+4815371 MsaT006377.1:CDS
ATCATCAGAACCACCACCAC+CGG 0.552134 2:-4814857 None:intergenic
CAATTGAAGAGAAAGGTTCG+TGG 0.552666 2:+4815084 MsaT006377.1:CDS
TGATGAGAATGAGGCGGAGG+AGG 0.559810 2:+4814876 MsaT006377.1:CDS
GATTCATCCTCAGAAGAAGA+AGG 0.562296 2:+4814451 MsaT006377.1:CDS
AATGCAGCTGCTTCCTTCAG+CGG 0.564243 2:-4815015 None:intergenic
TTAGGGTTAGGAGGAGGAGT+GGG 0.566966 2:-4814679 None:intergenic
GAGCCGGATTCAGATTCAGC+GGG 0.569467 2:-4814598 None:intergenic
TGATGATGAGAATGAGGCGG+AGG 0.577141 2:+4814873 MsaT006377.1:CDS
TCCTCAGAAGAAGAAGGAGA+TGG 0.578512 2:+4814457 MsaT006377.1:CDS
AAAAGCGGTTTGGAATTGAT+GGG 0.579284 2:+4815423 MsaT006377.1:CDS
TTCATGGGCTTTGAAGCAAG+TGG 0.579960 2:-4814709 None:intergenic
GAGGAGGATGTGAAATTGAC+TGG 0.583042 2:+4814892 MsaT006377.1:CDS
TCGTCGGAGGAAGAGGCGGT+GGG 0.588753 2:-4814370 None:intergenic
TGAAGGTGGTTGATCCTCGT+CGG 0.589745 2:-4814386 None:intergenic
GAAAGTGGAAAAGTGGAAAG+TGG 0.593025 2:+4815318 MsaT006377.1:CDS
AGCCCAAGCCCAATCAACTC+CGG 0.593390 2:+4814756 MsaT006377.1:CDS
TTTAGGGTTAGGAGGAGGAG+TGG 0.598971 2:-4814680 None:intergenic
ATCCTCGTCGGAGGAAGAGG+CGG 0.601599 2:-4814374 None:intergenic
CGTGCCGCCGAAAGTAACAC+TGG 0.604597 2:+4814802 MsaT006377.1:CDS
CACCGCCTCTTCCTCCGACG+AGG 0.604984 2:+4814372 MsaT006377.1:CDS
GGAAAAGTGGAAAGTGGTGT+TGG 0.606211 2:+4815324 MsaT006377.1:CDS
AAAGCGGTTTGGAATTGATG+GGG 0.609458 2:+4815424 MsaT006377.1:CDS
GACTGGAAAGGATCCGCTGA+AGG 0.611489 2:+4815002 MsaT006377.1:CDS
GTGGGTCGTCGACGAGAGCG+AGG 0.630460 2:-4814348 None:intergenic
TGGTTCTGATGATGAGAATG+AGG 0.631876 2:+4814867 MsaT006377.1:CDS
CGTTCCAAGAAGAAAACCAC+CGG 0.642479 2:+4814838 MsaT006377.1:CDS
GTTTCCAGTGTTACTTTCGG+CGG 0.643844 2:-4814806 None:intergenic
TGAGCCGGATTCAGATTCAG+CGG 0.650032 2:-4814599 None:intergenic
TTCAGATTCAGCGGGTTTAG+GGG 0.652803 2:-4814590 None:intergenic
GTAGAGATAGGAGTAGAAGG+AGG 0.653610 2:-4814568 None:intergenic
CGAACTGGTTAAATTTGATG+AGG 0.653994 2:+4815365 MsaT006377.1:CDS
ATCAGAACCACCACCACCGG+TGG 0.666036 2:-4814854 None:intergenic
AGGTGGTTGATCCTCGTCGG+AGG 0.667833 2:-4814383 None:intergenic
AAGCGGTTTGGAATTGATGG+GGG 0.669970 2:+4815425 MsaT006377.1:CDS
GAAGAAATCGATGAAAGCTG+AGG 0.677405 2:+4815050 MsaT006377.1:CDS
TCCAAGAAGAAAACCACCGG+TGG 0.717568 2:+4814841 MsaT006377.1:CDS
TTCTGATGATGAGAATGAGG+CGG 0.722855 2:+4814870 MsaT006377.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 4814319 4815587 4814319 ID=MsaG006377
Chr2 mRNA 4814319 4815587 4814319 ID=MsaT006377.1;Parent=MsaG006377
Chr2 exon 4814319 4815587 4814319 ID=MsaT006377.1.exon1;Parent=MsaT006377.1
Chr2 CDS 4814319 4815587 4814319 ID=cds.MsaT006377.1;Parent=MsaT006377.1
Gene Sequence

>MsaT006377.1

ATGGCGCCAAAGCCAAAGCAAAAGAAACGCCTCGCTCTCGTCGACGACCCACCCACCGCCTCTTCCTCCGACGAGGATCAACCACCTTCAACCCAAAAACCAGCCGACAAAGTAGTCAACGAAGAACATGAAGATTCATCCTCAGAAGAAGAAGGAGATGGAGAAGAAGAAGGTTCTTCATCTGAAGAAGAAGATAATCAAACTCAACAAACTCCTTCGAAAGATTCAAAACCCGCTTCCAAAAACCCTCCTCCTTCTACTCCTATCTCTACCCCTAAACCCGCTGAATCTGAATCCGGCTCAGAATCCGGTTCTGAATCTGGAACCGAATCTGATTCTGAACCGGAACAGAAATCGGAACCCACTCCTCCTCCTAACCCTAAAGTTAAACCACTTGCTTCAAAGCCCATGAAAACCCAAACCCAAGTCCAAGCCCAAGCCCAAGCCCAATCAACTCCGGTGCCAACCAAGTCCGGAACTAAGCGTGCCGCCGAAAGTAACACTGGAAACGATTCAAAACGTTCCAAGAAGAAAACCACCGGTGGTGGTGGTTCTGATGATGAGAATGAGGCGGAGGAGGATGTGAAATTGACTGGTGAAGACTCGAAGAAGAACTTTCAGAGATTGTTTAGTGAAGAAGACGAGCTTGTGATTCTCAAAGGGCTTGGGGATTTTGTGGCGAAGACTGGAAAGGATCCGCTGAAGGAAGCAGCTGCATTTCACGGTTTCGTGAAGAAATCGATGAAAGCTGAGGCCAATGCTCAGCAATTGAAGAGAAAGGTTCGTGGTTTGAAGAAGAAGTTTGAAAGCAGTGACAATTTTACCAAAGCTCATGATAAGAGAGCATTTGATCTTTTCAAGGTTTGGAGCAAAAATGCTTCTAAGGAAGCTGAAGAAAACGGAAAATCTAACGAGAAAATCACAAAGAGTGCTAAGAAGGAAGCTCCTGTTAAAAATGGTGGTTCTGCTAGGAAGGAATTAGCTATAGTTACTAAGAATGAAAGTGGAAAAGTGGAAAGTGGTGTTGGCAAGAGTTTCATTTTGAGCGAACTGGTTAAATTTGATGAGGCTGCGGGTATAGCTGTGCTGGATAAGGATGCTACAAAAAGCGGTTTGGAATTGATGGGGGAGTCAGATAGGGTTGAGTTGGAGGAGAGGTGGAAGAAGTTGCAGGCTGCCGAGATGGAAGTGTTTTTGAATCGCGCTGAGGTGGTTAGGGAGCAGACTAGATTGGCATTGCAGGAACTCAAGAAGAAATCAAGCAAATAG

Protein sequence

>MsaT006377.1

MAPKPKQKKRLALVDDPPTASSSDEDQPPSTQKPADKVVNEEHEDSSSEEEGDGEEEGSSSEEEDNQTQQTPSKDSKPASKNPPPSTPISTPKPAESESGSESGSESGTESDSEPEQKSEPTPPPNPKVKPLASKPMKTQTQVQAQAQAQSTPVPTKSGTKRAAESNTGNDSKRSKKKTTGGGGSDDENEAEEDVKLTGEDSKKNFQRLFSEEDELVILKGLGDFVAKTGKDPLKEAAAFHGFVKKSMKAEANAQQLKRKVRGLKKKFESSDNFTKAHDKRAFDLFKVWSKNASKEAEENGKSNEKITKSAKKEAPVKNGGSARKELAIVTKNESGKVESGVGKSFILSELVKFDEAAGIAVLDKDATKSGLELMGESDRVELEERWKKLQAAEMEVFLNRAEVVREQTRLALQELKKKSSK*