AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006437

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006437 MtrunA17_Chr2g0281191 95.991 848 22 2 18 865 62 897 0.0 1670
MsaG006437 MtrunA17_Chr4g0012861 51.701 588 214 13 275 811 270 838 0.0 549
MsaG006437 MtrunA17_Chr4g0012861 46.377 69 33 1 7 71 24 92 1.75e-15 81.3
MsaG006437 MtrunA17_Chr3g0107701 40.976 820 392 17 18 785 49 828 4.53e-178 537
MsaG006437 MtrunA17_Chr5g0432721 39.964 548 230 12 281 785 283 774 8.08e-104 339
MsaG006437 MtrunA17_Chr5g0432721 50.847 59 29 0 19 77 40 98 7.66e-13 72.4
MsaG006437 MtrunA17_Chr7g0244871 67.391 92 30 0 313 404 6 97 9.49e-40 142
MsaG006437 MtrunA17_Chr7g0251891 39.394 132 71 3 319 446 16 142 4.84e-20 89.4
MsaG006437 MtrunA17_Chr7g0237841 38.235 102 61 2 319 418 623 724 2.00e-15 80.9
MsaG006437 MtrunA17_Chr8g0345771 38.182 110 60 4 319 422 171 278 3.07e-14 76.6
MsaG006437 MtrunA17_Chr1g0178071 35.484 124 72 4 302 419 58 179 5.67e-14 74.3
MsaG006437 MtrunA17_Chr7g0276471 38.679 106 57 4 319 418 117 220 1.97e-13 73.6
MsaG006437 MtrunA17_Chr7g0266821 41.000 100 52 5 324 419 65 161 8.63e-13 70.1
MsaG006437 MtrunA17_Chr1g0198661 33.813 139 72 5 319 439 191 327 4.63e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006437 AT4G32010.2 52.990 485 218 6 275 751 237 719 1.43e-158 483
MsaG006437 AT4G32010.2 50.909 55 27 0 19 73 42 96 7.36e-14 76.3
MsaG006437 AT4G32010.1 52.556 489 218 7 275 751 237 723 3.06e-156 477
MsaG006437 AT4G32010.1 50.909 55 27 0 19 73 42 96 7.20e-14 76.3
MsaG006437 AT2G30470.1 48.902 501 235 5 268 752 243 738 2.48e-143 444
MsaG006437 AT2G30470.1 55.172 58 25 1 15 71 40 97 1.65e-13 75.1
MsaG006437 AT4G21550.3 40.374 535 245 17 18 536 50 526 6.71e-104 333
MsaG006437 AT4G21550.1 40.429 559 255 18 18 560 50 546 2.66e-100 328
MsaG006437 AT4G21550.1 47.020 151 51 4 642 792 541 662 1.69e-21 100
MsaG006437 AT4G21550.2 39.923 521 239 17 18 522 50 512 4.33e-98 317
MsaG006437 AT3G26790.1 41.346 104 60 1 319 421 91 194 3.28e-19 90.1
MsaG006437 AT3G24650.1 35.172 145 76 4 282 418 538 672 4.76e-16 83.2
MsaG006437 AT2G36080.1 39.815 108 56 4 319 419 37 142 6.72e-15 75.9
MsaG006437 AT2G36080.3 39.815 108 56 4 319 419 37 142 6.99e-15 75.5
MsaG006437 AT5G06250.5 37.391 115 56 4 319 419 45 157 7.07e-15 76.3
MsaG006437 AT5G06250.4 37.391 115 56 4 319 419 45 157 8.37e-15 76.3
MsaG006437 AT2G36080.4 39.815 108 56 4 319 419 37 142 8.81e-15 73.6
MsaG006437 AT2G36080.2 39.815 108 56 4 319 419 37 142 8.81e-15 73.6
MsaG006437 AT5G06250.2 37.391 115 56 4 319 419 45 157 9.70e-15 76.3
MsaG006437 AT5G06250.3 37.391 115 56 4 319 419 45 157 1.00e-14 75.9
MsaG006437 AT5G06250.1 37.391 115 56 4 319 419 45 157 1.25e-14 75.5
MsaG006437 AT1G28300.1 39.000 100 59 2 306 404 158 256 4.03e-14 75.5
MsaG006437 AT4G01500.1 40.449 89 50 2 319 406 35 121 2.71e-13 72.4
MsaG006437 AT2G46870.1 39.560 91 50 3 319 406 34 122 9.96e-13 70.5
MsaG006437 AT3G11580.4 36.207 116 57 4 319 419 28 141 1.29e-12 68.9
MsaG006437 AT3G11580.2 36.207 116 57 4 319 419 28 141 1.29e-12 68.9
MsaG006437 AT3G11580.5 36.207 116 57 4 319 419 28 141 1.29e-12 68.9
MsaG006437 AT3G11580.3 36.207 116 57 4 319 419 28 141 3.45e-12 69.7
MsaG006437 AT3G25730.1 39.362 94 49 4 319 406 182 273 5.22e-12 68.6
MsaG006437 AT3G11580.1 36.207 116 57 4 319 419 28 141 6.27e-12 67.4
MsaG006437 AT1G25560.1 34.746 118 63 5 319 424 194 309 6.60e-12 68.6

Find 226 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACATTCTGCTGTTGTTATT+TGG 0.148216 2:-5517916 None:intergenic
GGATCACTCTCGTACCTTTC+TGG 0.160003 2:-5521084 None:intergenic
AATGAAAGAAATGGTCAAAT+TGG 0.226571 2:+5516307 MsaT006437.1:CDS
CTTTCATTTGGGGATGAATA+AGG 0.231314 2:-5516292 None:intergenic
TTGTCGCCTGGTTCAGATTC+TGG 0.241849 2:+5522093 MsaT006437.1:CDS
ATTTGACCATTTCTTTCATT+TGG 0.245941 2:-5516304 None:intergenic
AGATCAATTTGGCCTTTAAA+TGG 0.248134 2:-5522042 None:intergenic
ATCTCTTACAAATCGTTTAT+CGG 0.260733 2:+5515774 MsaT006437.1:CDS
TTTGACCATTTCTTTCATTT+GGG 0.271314 2:-5516303 None:intergenic
TGTCTGGAATATCAGCTAAT+TGG 0.273177 2:+5516010 MsaT006437.1:CDS
GGATATTTCAGTTCCGCTTC+TGG 0.277425 2:+5517893 MsaT006437.1:CDS
AACCCTTCAGGCTGAGAAAT+TGG 0.301947 2:-5517835 None:intergenic
GCTGGATATTGAGATCAATT+TGG 0.303683 2:-5522053 None:intergenic
ATCATTACCCTCCTTGTTCA+TGG 0.305949 2:-5521977 None:intergenic
AATGGTCAAATTGGGGTAAC+TGG 0.309222 2:+5516316 MsaT006437.1:CDS
GCCAGTGATGCTGGGCGAAT+TGG 0.316030 2:+5516696 MsaT006437.1:CDS
CAGACTGGGTGCTTGCTTCC+AGG 0.319580 2:-5515857 None:intergenic
TACCCTCCTTGTTCATGGTT+GGG 0.322992 2:-5521972 None:intergenic
GCCTGAAGGGAGGTTGGTTA+TGG 0.323362 2:+5518866 MsaT006437.1:CDS
TCATCCGTGTCTCCATTTAA+AGG 0.326774 2:+5522030 MsaT006437.1:CDS
AATAACTTTGACAAAATATA+TGG 0.331049 2:+5515940 MsaT006437.1:CDS
ATCTGTCGCTTAGGTTGAAT+TGG 0.334190 2:+5519623 MsaT006437.1:intron
AGCTAATTGGAATGTCAAAC+TGG 0.337122 2:+5516023 MsaT006437.1:CDS
GTGCACTTCTTCCAGCAAAA+TGG 0.337367 2:+5521037 MsaT006437.1:CDS
ATGAAAGAAATGGTCAAATT+GGG 0.345350 2:+5516308 MsaT006437.1:CDS
GGCCCTTTCCACTAGGCGGC+TGG 0.351565 2:-5521738 None:intergenic
CCAGTGATGCTGGGCGAATT+GGG 0.353628 2:+5516697 MsaT006437.1:CDS
GTGCATGTCTGCTTATGCTT+GGG 0.356470 2:-5521760 None:intergenic
GTGCCCATAAAATGTTATTC+TGG 0.358593 2:+5514923 MsaT006437.1:CDS
TCCTGATCATGCTGAAGCTT+TGG 0.362667 2:+5521597 MsaT006437.1:CDS
CCTGAAGGGAGGTTGGTTAT+GGG 0.369927 2:+5518867 MsaT006437.1:CDS
TTTCAGATGAAGGCAGTTGC+TGG 0.379421 2:-5521900 None:intergenic
CTTAGCAACGGTGTTATTCA+TGG 0.385576 2:+5522252 MsaT006437.1:CDS
TCTTCTCACGCCGTAACATA+AGG 0.386896 2:-5521822 None:intergenic
AAGTAACACCTTTGTGTTGT+TGG 0.387030 2:+5514873 MsaT006437.1:CDS
AATTGGCTGATCCTAGTTCC+TGG 0.387306 2:+5519640 MsaT006437.1:CDS
CCCATAACCAACCTCCCTTC+AGG 0.389700 2:-5518867 None:intergenic
GTGGGTGTTTAGTTGTGGCC+TGG 0.391891 2:-5521681 None:intergenic
TTACAGGATGCTCCGGTGCT+TGG 0.393729 2:+5520520 MsaT006437.1:intron
GTTGGTTATGGGATTTAGAA+AGG 0.394057 2:+5518878 MsaT006437.1:CDS
CCCAATTCGCCCAGCATCAC+TGG 0.395558 2:-5516697 None:intergenic
GGACATGTTCATATAATTCA+TGG 0.400111 2:+5521058 MsaT006437.1:CDS
ACCTTTGTGTTGTTGGATCC+AGG 0.400804 2:+5514880 MsaT006437.1:CDS
GTCAGTTGTTGCCACGGTAT+TGG 0.401190 2:+5516467 MsaT006437.1:CDS
TGTGGCCACGGCAGGTTAGA+TGG 0.401999 2:-5515745 None:intergenic
ACAATGCTTGGGACATGATT+AGG 0.404239 2:-5519843 None:intergenic
TTACCCTCCTTGTTCATGGT+TGG 0.412271 2:-5521973 None:intergenic
TACAGGATGCTCCGGTGCTT+GGG 0.413480 2:+5520521 MsaT006437.1:intron
TTCCTTGCATAATAGTAATA+CGG 0.417300 2:+5521939 MsaT006437.1:CDS
TTGGCCCAGGTGTACTGAAT+TGG 0.418132 2:+5516486 MsaT006437.1:CDS
GATTCATCTTCTCAACCGTT+TGG 0.422726 2:+5516259 MsaT006437.1:CDS
CGCAGTAACATTTAGCCGGT+TGG 0.424036 2:+5518842 MsaT006437.1:intron
GACAATTCTTCTTCTCGCTC+TGG 0.431144 2:-5522075 None:intergenic
ACTTGCAAATCTAGCAATCC+TGG 0.433506 2:+5521633 MsaT006437.1:CDS
CCTTCAATCACCACAATGCT+TGG 0.436508 2:-5519855 None:intergenic
CTTTGAGTTTGAGGAATATG+AGG 0.436772 2:+5519878 MsaT006437.1:CDS
ACTTTGAGTGCCAGTGATGC+TGG 0.442164 2:+5516687 MsaT006437.1:CDS
CATCTAGGCAAGCAGTTTAG+TGG 0.442863 2:+5516364 MsaT006437.1:CDS
CATCCCAGAGCAGTTATGTA+TGG 0.443039 2:+5514140 MsaT006437.1:CDS
GGTGCTTGCTTCCAGGGTAC+AGG 0.444210 2:-5515850 None:intergenic
CCTTTAGAGTATGAGGCTGT+TGG 0.444476 2:-5516234 None:intergenic
ACACGTTCATAGACAAAGAC+TGG 0.448302 2:-5522308 None:intergenic
CATTTACCTCTTGACTCATC+AGG 0.448985 2:+5516394 MsaT006437.1:CDS
TGTGCATGTCTGCTTATGCT+TGG 0.449862 2:-5521761 None:intergenic
AAATCCTTTGCCATCCGATC+AGG 0.452416 2:+5518905 MsaT006437.1:CDS
TGGGTGTTTAGTTGTGGCCT+GGG 0.452762 2:-5521680 None:intergenic
CAGTAGTCATAATGCTGATA+AGG 0.453305 2:+5522278 MsaT006437.1:CDS
TGCTGGGCGAATTGGGCGCT+TGG 0.455987 2:+5516704 MsaT006437.1:CDS
TCAACTAGAGAATGCACATC+TGG 0.456237 2:-5515910 None:intergenic
TCATCCCCAAATGAAAGAAA+TGG 0.456820 2:+5516298 MsaT006437.1:CDS
TGATCATGCTGAAGCTTTGG+AGG 0.457477 2:+5521600 MsaT006437.1:CDS
TCAACATTTGACCAGGAACT+AGG 0.459457 2:-5519651 None:intergenic
CTTAAAATCCTCGATGCAAA+AGG 0.461243 2:+5517862 MsaT006437.1:CDS
CAACAAACTCCACCTCATCT+AGG 0.464917 2:+5516349 MsaT006437.1:CDS
TGGTCAAATGTTGATAAGTC+AGG 0.467081 2:+5519660 MsaT006437.1:CDS
GGAATGTCAAACTGGGTTCA+AGG 0.467906 2:+5516031 MsaT006437.1:CDS
ATTCTTCTTCTCGCTCTGGC+TGG 0.467907 2:-5522071 None:intergenic
TTAGTTGTGGCCTGGGAAGA+TGG 0.468284 2:-5521673 None:intergenic
GCAAACAATGCAAGAGCAGC+CGG 0.469885 2:-5521713 None:intergenic
GCAACAACTGACTTCTTCCT+CGG 0.470752 2:-5516456 None:intergenic
GAATCTTCCTTTAGAGTATG+AGG 0.472283 2:-5516241 None:intergenic
TGACTCATCAGGTGAAGCTC+AGG 0.473085 2:+5516405 MsaT006437.1:CDS
TGGAGTCAGTTAGCTGGATC+AGG 0.473225 2:+5515826 MsaT006437.1:CDS
CCAAGCATTGTGGTGATTGA+AGG 0.474053 2:+5519855 MsaT006437.1:CDS
ATGAGGAATGAGGACAAATC+AGG 0.474622 2:+5516193 MsaT006437.1:CDS
ACCTCCCTTCAGGCTCCAAC+CGG 0.476212 2:-5518857 None:intergenic
TTATCACTAGTGAAAATAGA+TGG 0.477763 2:-5520547 None:intergenic
CCACCAATTTCTCAGCCTGA+AGG 0.479735 2:+5517832 MsaT006437.1:CDS
TCCAAAGCTTCAGCATGATC+AGG 0.479989 2:-5521598 None:intergenic
ATCCAGCCGCCTAGTGGAAA+GGG 0.481833 2:+5521736 MsaT006437.1:CDS
GATAATGAAGCTAATAAGAC+TGG 0.483266 2:+5519505 MsaT006437.1:CDS
ACTCAAACGCTTCTGGTTGG+AGG 0.485042 2:+5514670 MsaT006437.1:CDS
GTTTCCGTACCCTTATGTTA+CGG 0.488608 2:+5521812 MsaT006437.1:CDS
CCATTCAAACCTGAATTACA+AGG 0.491131 2:-5521426 None:intergenic
CTGGATCAGGACCTGTACCC+TGG 0.491579 2:+5515839 MsaT006437.1:CDS
GAAGCTAATAAGACTGGAAA+CGG 0.491637 2:+5519511 MsaT006437.1:CDS
TCTGGTACTGGAGATTCATC+AGG 0.492385 2:+5522186 MsaT006437.1:CDS
TCAAGCAAAATTTCAGATGA+AGG 0.493848 2:-5521910 None:intergenic
GTCACCTGCTTGCAACTGCA+TGG 0.495052 2:-5517971 None:intergenic
GAAAATGAAGGCCACTAAGC+AGG 0.495097 2:+5521573 MsaT006437.1:CDS
GATTGAAGGCTTTGAGTTTG+AGG 0.495120 2:+5519869 MsaT006437.1:CDS
TGTTGCCACGGTATTGGCCC+AGG 0.495577 2:+5516473 MsaT006437.1:CDS
TAGCTCCAATTCAGTACACC+TGG 0.498477 2:-5516491 None:intergenic
ATAAAGATTGTGGCCACGGC+AGG 0.499237 2:-5515753 None:intergenic
CTTCTCACGCCGTAACATAA+GGG 0.500342 2:-5521821 None:intergenic
ATGATCAGGATCCTGCTTAG+TGG 0.500883 2:-5521584 None:intergenic
AGACTGGGTGCTTGCTTCCA+GGG 0.501160 2:-5515856 None:intergenic
CTAACCATACATAACTGCTC+TGG 0.501950 2:-5514144 None:intergenic
GGAGGCTCTCCCATCTTCCC+AGG 0.502386 2:+5521663 MsaT006437.1:CDS
CTGAGAAATTGGTGGGAAGT+AGG 0.504512 2:-5517824 None:intergenic
GAGCAGCCGGGTCTATGTCG+TGG 0.505284 2:-5521700 None:intergenic
ACAAAATATATGGCAATGAA+AGG 0.507284 2:+5515950 MsaT006437.1:CDS
TTCAGATGAAGGCAGTTGCT+GGG 0.508534 2:-5521899 None:intergenic
TCCTTGCATAATAGTAATAC+GGG 0.509224 2:+5521940 MsaT006437.1:CDS
CATCCAGCCGCCTAGTGGAA+AGG 0.510624 2:+5521735 MsaT006437.1:CDS
GAGACATGTCTGGCAAAAGT+TGG 0.514952 2:+5515806 MsaT006437.1:CDS
TTTGTTGTATTGCAGGAATG+AGG 0.515892 2:+5516176 MsaT006437.1:intron
ATCAGGTAGTCAGTCACAGC+AGG 0.516556 2:+5522203 MsaT006437.1:CDS
TAACCATACATAACTGCTCT+GGG 0.521395 2:-5514143 None:intergenic
CCAACAGCCTCATACTCTAA+AGG 0.524975 2:+5516234 MsaT006437.1:CDS
ACTTTGTTGAACTCTGGTAC+TGG 0.527710 2:+5522174 MsaT006437.1:CDS
ATACATAGCAAAAGAAGCAC+TGG 0.528619 2:+5519683 MsaT006437.1:CDS
CAGCAGACTTTGTTGAACTC+TGG 0.530003 2:+5522168 MsaT006437.1:CDS
TCCACTCAAACGCTTCTGGT+TGG 0.530665 2:+5514667 MsaT006437.1:CDS
GTAAAGCATTCAATTCCTCC+TGG 0.532051 2:-5514898 None:intergenic
CTTGCAAATCTAGCAATCCT+GGG 0.532774 2:+5521634 MsaT006437.1:CDS
CTTCAATCACCACAATGCTT+GGG 0.534367 2:-5519854 None:intergenic
TGCTTGGGGCCCTTTCCACT+AGG 0.534401 2:-5521745 None:intergenic
ATGAACAAGGAGGGTAATGA+TGG 0.534494 2:+5521979 MsaT006437.1:CDS
GAGAGATATAGAGACATGTC+TGG 0.539014 2:+5515796 MsaT006437.1:CDS
GAATTGTGAGACTTGTAGAA+AGG 0.540816 2:+5514692 MsaT006437.1:CDS
CACCAATTTCTCAGCCTGAA+GGG 0.542196 2:+5517833 MsaT006437.1:CDS
CAACAACTGACTTCTTCCTC+GGG 0.544517 2:-5516455 None:intergenic
GTGAAGCTCAGGTTCGCAAT+GGG 0.546771 2:+5516416 MsaT006437.1:CDS
CAGTCCATGCAGTTGCAAGC+AGG 0.549929 2:+5517967 MsaT006437.1:CDS
GAATGTCAAACTGGGTTCAA+GGG 0.552101 2:+5516032 MsaT006437.1:CDS
GAGAATGCACATCTGGAGGC+TGG 0.553030 2:-5515903 None:intergenic
TAGTTGTGGCCTGGGAAGAT+GGG 0.553270 2:-5521672 None:intergenic
TGCATGTCTGCTTATGCTTG+GGG 0.556813 2:-5521759 None:intergenic
CGAGAAGAAGAATTGTCGCC+TGG 0.557693 2:+5522081 MsaT006437.1:CDS
AGAGATAAAGATTGTGGCCA+CGG 0.559976 2:-5515757 None:intergenic
ATAATTCATGGGATCCAGAA+AGG 0.562018 2:+5521070 MsaT006437.1:CDS
GTTGGAGCCTGAAGGGAGGT+TGG 0.564406 2:+5518860 MsaT006437.1:CDS
GACATGTTCATATAATTCAT+GGG 0.566869 2:+5521059 MsaT006437.1:CDS
AAAAGTTGGAGTCAGTTAGC+TGG 0.568196 2:+5515820 MsaT006437.1:CDS
AGAAGCTGAATTGAACAGAC+TGG 0.568636 2:-5515872 None:intergenic
AACTGCTTGCCTAGATGAGG+TGG 0.570136 2:-5516358 None:intergenic
TCCAACCAGAAGCGTTTGAG+TGG 0.572844 2:-5514668 None:intergenic
TGCTTGGGACATGATTAGGA+GGG 0.573196 2:-5519839 None:intergenic
TAATAGAGCTGAAGATTACA+TGG 0.574564 2:+5519790 MsaT006437.1:CDS
AGCAGCCGGGTCTATGTCGT+GGG 0.575738 2:-5521699 None:intergenic
TTTAGCCGGTTGGAGCCTGA+AGG 0.576874 2:+5518852 MsaT006437.1:CDS
ATAGATGGCTTCCCAAGCAC+CGG 0.579291 2:-5520532 None:intergenic
CAATGCAGATATTACCTGAT+CGG 0.581192 2:-5518919 None:intergenic
GCAGATATTACCTGATCGGA+TGG 0.582173 2:-5518915 None:intergenic
ATGTCGTGGGTGTTTAGTTG+TGG 0.582924 2:-5521686 None:intergenic
CCTTCAGGCTGAGAAATTGG+TGG 0.583560 2:-5517832 None:intergenic
GGTGAAGCTCAGGTTCGCAA+TGG 0.584201 2:+5516415 MsaT006437.1:CDS
TGTTGTTATTTGGCCAGAAG+CGG 0.585150 2:-5517906 None:intergenic
TAAGGTACAGATAAACCAAA+CGG 0.588759 2:-5516274 None:intergenic
GATCATGCTGAAGCTTTGGA+GGG 0.590184 2:+5521601 MsaT006437.1:CDS
ACCCGTATTACTATTATGCA+AGG 0.591046 2:-5521941 None:intergenic
GCAGGTAGGAGATGTTGTGA+GGG 0.592609 2:+5522221 MsaT006437.1:CDS
AGGGACATGTTGCTTATGAA+CGG 0.593802 2:+5516051 MsaT006437.1:CDS
AATCCTCGATGCAAAAGGAA+AGG 0.594386 2:+5517867 MsaT006437.1:CDS
TACATAGCAAAAGAAGCACT+GGG 0.595326 2:+5519684 MsaT006437.1:CDS
AGCAGTTTCCAAGAAAATGA+AGG 0.595741 2:+5521561 MsaT006437.1:intron
TTGACCATTTCTTTCATTTG+GGG 0.596631 2:-5516302 None:intergenic
GCTGGAAGAAGTGCACATGC+TGG 0.600458 2:-5521030 None:intergenic
CAGTTGCAAGCAGGTGACAC+AGG 0.601495 2:+5517976 MsaT006437.1:CDS
GTTTGCATCCAGCCGCCTAG+TGG 0.603172 2:+5521730 MsaT006437.1:CDS
CAAACAATGCAAGAGCAGCC+GGG 0.603823 2:-5521712 None:intergenic
CTTCCTCGGGCATCTGCTCG+AGG 0.605073 2:-5516442 None:intergenic
ACACCACCAGAATCTGAACC+AGG 0.607711 2:-5522099 None:intergenic
ATTACCTGATCGGATGGCAA+AGG 0.607719 2:-5518909 None:intergenic
CTCAGCCATCTAACCTGCCG+TGG 0.607811 2:+5515740 MsaT006437.1:CDS
ATGCACATCTGGAGGCTGGT+CGG 0.608646 2:-5515899 None:intergenic
TGACTTATCAACATTTGACC+AGG 0.608800 2:-5519658 None:intergenic
GCCGGTTGGAGCCTGAAGGG+AGG 0.610013 2:+5518856 MsaT006437.1:CDS
TAGTAGCCCAACCATGAACA+AGG 0.610538 2:+5521966 MsaT006437.1:CDS
TTAGCCGGTTGGAGCCTGAA+GGG 0.610617 2:+5518853 MsaT006437.1:CDS
ATGCTTGGGACATGATTAGG+AGG 0.611515 2:-5519840 None:intergenic
CTTCAGGCTGAGAAATTGGT+GGG 0.611961 2:-5517831 None:intergenic
CACGTTCATAGACAAAGACT+GGG 0.613315 2:-5522307 None:intergenic
TGAAAGAAATGGTCAAATTG+GGG 0.613362 2:+5516309 MsaT006437.1:CDS
CTTTGAGTGCCAGTGATGCT+GGG 0.613991 2:+5516688 MsaT006437.1:CDS
TTTGTAAGAGATAAAGATTG+TGG 0.616956 2:-5515763 None:intergenic
GAAGAAGTCAGTTGTTGCCA+CGG 0.618200 2:+5516461 MsaT006437.1:CDS
CTAAACTGCTTGCCTAGATG+AGG 0.618575 2:-5516361 None:intergenic
TCGATGCAAAAGGAAAGGAA+TGG 0.623875 2:+5517872 MsaT006437.1:CDS
ATTACATGGCAAGAAGCTCA+AGG 0.624749 2:+5519804 MsaT006437.1:CDS
AGCAGGTAGGAGATGTTGTG+AGG 0.624795 2:+5522220 MsaT006437.1:CDS
CTTGGGACATGATTAGGAGG+GGG 0.628705 2:-5519837 None:intergenic
AGCTCCAATTCAGTACACCT+GGG 0.629096 2:-5516490 None:intergenic
AATGTGAACATTGTCTGAAG+TGG 0.629729 2:+5520998 MsaT006437.1:CDS
CGACCTCGAGCAGATGCCCG+AGG 0.632234 2:+5516439 MsaT006437.1:CDS
GGTAGTCAGTCACAGCAGGT+AGG 0.633855 2:+5522207 MsaT006437.1:CDS
GCTTCACCTGATGAGTCAAG+AGG 0.634530 2:-5516400 None:intergenic
GCTAATTGGAATGTCAAACT+GGG 0.636082 2:+5516024 MsaT006437.1:CDS
TATTGCAGGAATGAGGAATG+AGG 0.637424 2:+5516183 MsaT006437.1:intron
TTGGCCTTTAAATGGAGACA+CGG 0.638401 2:-5522034 None:intergenic
TTGCAAATCTAGCAATCCTG+GGG 0.642083 2:+5521635 MsaT006437.1:CDS
TAATCATGTCCCAAGCATTG+TGG 0.643328 2:+5519845 MsaT006437.1:CDS
AGCCCAACCATGAACAAGGA+GGG 0.646404 2:+5521970 MsaT006437.1:CDS
GTTCTGATGATGATCCTCAC+CGG 0.646876 2:+5522001 MsaT006437.1:CDS
ATTCAAACCTGAATTACAAG+GGG 0.647708 2:-5521424 None:intergenic
CATTCAAACCTGAATTACAA+GGG 0.647900 2:-5521425 None:intergenic
GCTTGGGACATGATTAGGAG+GGG 0.648870 2:-5519838 None:intergenic
ACTAGAGAATGCACATCTGG+AGG 0.649096 2:-5515907 None:intergenic
CACGCCGTAACATAAGGGTA+CGG 0.656740 2:-5521816 None:intergenic
GAAGCTGAATTGAACAGACT+GGG 0.665746 2:-5515871 None:intergenic
AAACACCCACGACATAGACC+CGG 0.669434 2:+5521694 MsaT006437.1:CDS
AACTGCATGGACTGTATGCA+CGG 0.670080 2:-5517958 None:intergenic
AGGGAAGTGAAGCTTAGCAA+CGG 0.671793 2:+5522240 MsaT006437.1:CDS
CACATGTAATGTCTGTCTAA+CGG 0.673914 2:+5521780 MsaT006437.1:CDS
TTGGGGCCCTTTCCACTAGG+CGG 0.682669 2:-5521742 None:intergenic
TGTTGGCACATATTTAAACA+CGG 0.683366 2:-5516217 None:intergenic
TCGCCTGGTTCAGATTCTGG+TGG 0.694439 2:+5522096 MsaT006437.1:CDS
ACATTTCAGCAGCAGCATCG+TGG 0.696358 2:-5522134 None:intergenic
GTACACCTGGGCCAATACCG+TGG 0.704903 2:-5516478 None:intergenic
TTTGTGTTGTTGGATCCAGG+AGG 0.707436 2:+5514883 MsaT006437.1:CDS
TCCTGGATCCAACAACACAA+AGG 0.713489 2:-5514881 None:intergenic
TAGCCCAACCATGAACAAGG+AGG 0.716754 2:+5521969 MsaT006437.1:CDS
TGCAAATCTAGCAATCCTGG+GGG 0.721871 2:+5521636 MsaT006437.1:CDS
TTCAAACCTGAATTACAAGG+GGG 0.743313 2:-5521423 None:intergenic
ACATAGCAAAAGAAGCACTG+GGG 0.799937 2:+5519685 MsaT006437.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 5514112 5522332 5514112 ID=MsaG006437
Chr2 mRNA 5514112 5522332 5514112 ID=MsaT006437.1;Parent=MsaG006437
Chr2 exon 5514112 5514161 5514112 ID=MsaT006437.1.exon1;Parent=MsaT006437.1
Chr2 CDS 5514112 5514161 5514112 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5514632 5514713 5514632 ID=MsaT006437.1.exon2;Parent=MsaT006437.1
Chr2 CDS 5514632 5514713 5514632 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5514847 5514944 5514847 ID=MsaT006437.1.exon3;Parent=MsaT006437.1
Chr2 CDS 5514847 5514944 5514847 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5515711 5516072 5515711 ID=MsaT006437.1.exon4;Parent=MsaT006437.1
Chr2 CDS 5515711 5516072 5515711 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5516191 5516530 5516191 ID=MsaT006437.1.exon5;Parent=MsaT006437.1
Chr2 CDS 5516191 5516530 5516191 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5516653 5516749 5516653 ID=MsaT006437.1.exon6;Parent=MsaT006437.1
Chr2 CDS 5516653 5516749 5516653 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5517823 5517997 5517823 ID=MsaT006437.1.exon7;Parent=MsaT006437.1
Chr2 CDS 5517823 5517997 5517823 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5518847 5518926 5518847 ID=MsaT006437.1.exon8;Parent=MsaT006437.1
Chr2 CDS 5518847 5518926 5518847 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5519505 5519549 5519505 ID=MsaT006437.1.exon9;Parent=MsaT006437.1
Chr2 CDS 5519505 5519549 5519505 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5519636 5519899 5519636 ID=MsaT006437.1.exon10;Parent=MsaT006437.1
Chr2 CDS 5519636 5519899 5519636 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5520526 5520575 5520526 ID=MsaT006437.1.exon11;Parent=MsaT006437.1
Chr2 CDS 5520526 5520575 5520526 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5520978 5521091 5520978 ID=MsaT006437.1.exon12;Parent=MsaT006437.1
Chr2 CDS 5520978 5521091 5520978 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5521368 5521438 5521368 ID=MsaT006437.1.exon13;Parent=MsaT006437.1
Chr2 CDS 5521368 5521438 5521368 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Chr2 exon 5521563 5522332 5521563 ID=MsaT006437.1.exon14;Parent=MsaT006437.1
Chr2 CDS 5521563 5522332 5521563 ID=cds.MsaT006437.1;Parent=MsaT006437.1
Gene Sequence

>MsaT006437.1

ATGGCTCTGATCAATAATTTTCTATATTCATCCCAGAGCAGTTATGTATGTTCTGCTTTTGAAGAAGGGAGATTCTGTGACATTTTCCACTCAAACGCTTCTGGTTGGAGGAATTGTGAGACTTGTAGAAAGCGAATTCACTGTGGGTGTATTGTTTCAAGTAACACCTTTGTGTTGTTGGATCCAGGAGGAATTGAATGCTTTACATGTGCCCATAAAATGTTATTCTGTGACTTTTCTCTTTATCTATTATCTTTATCTCAGCCATCTAACCTGCCGTGGCCACAATCTTTATCTCTTACAAATCGTTTATCGGAGAGATATAGAGACATGTCTGGCAAAAGTTGGAGTCAGTTAGCTGGATCAGGACCTGTACCCTGGAAGCAAGCACCCAGTCTGTTCAATTCAGCTTCTTCATCCGACCAGCCTCCAGATGTGCATTCTCTAGTTGATTTATCAAATAACTTTGACAAAATATATGGCAATGAAAGGTTGCCCCCCTCATCATTGGAAAAGAAAAATGAGGATTTGTCTGGAATATCAGCTAATTGGAATGTCAAACTGGGTTCAAGGGACATGTTGCTTATGAACGGAATGAGGAATGAGGACAAATCAGGTCCGTGTTTAAATATGTGCCAACAGCCTCATACTCTAAAGGAAGATTCATCTTCTCAACCGTTTGGTTTATCTGTACCTTATTCATCCCCAAATGAAAGAAATGGTCAAATTGGGGTAACTGGAATTCATCTACAACAAACTCCACCTCATCTAGGCAAGCAGTTTAGTGGCACTATGCATTTACCTCTTGACTCATCAGGTGAAGCTCAGGTTCGCAATGGGCGACCTCGAGCAGATGCCCGAGGAAGAAGTCAGTTGTTGCCACGGTATTGGCCCAGGTGTACTGAATTGGAGCTACAGCAAATATCTGTCGATTCTAATTCTGTAATAACTCCCTTGTTCCAAAAAACTTTGAGTGCCAGTGATGCTGGGCGAATTGGGCGCTTGGTACTACCCAAAAAATGTGCTGAGACCTACTTCCCACCAATTTCTCAGCCTGAAGGGTTGCCTCTTAAAATCCTCGATGCAAAAGGAAAGGAATGGATATTTCAGTTCCGCTTCTGGCCAAATAACAACAGCAGAATGTATGTTTTAGAGGGTGTAACTCCGTGCATACAGTCCATGCAGTTGCAAGCAGGTGACACAGTAACATTTAGCCGGTTGGAGCCTGAAGGGAGGTTGGTTATGGGATTTAGAAAGGCTACAAATCCTTTGCCATCCGATCAGGATAATGAAGCTAATAAGACTGGAAACGGATTTTCTGCTCACGAAGTTGAATTGGCTGATCCTAGTTCCTGGTCAAATGTTGATAAGTCAGGATACATAGCAAAAGAAGCACTGGGGAGCAAATCTTTAATCTCTAGAAAGAGAAAAAACAACATATTGGGTTCAAAAAGTAAACGTCTAAAAATTGAAAATGAGGATATAATAGAGCTGAAGATTACATGGCAAGAAGCTCAAGGCCTGCTTCGTCCCCCTCCTAATCATGTCCCAAGCATTGTGGTGATTGAAGGCTTTGAGTTTGAGGAATATGAGGATGCTCCGGTGCTTGGGAAGCCATCTATTTTCACTAGTGATAATATGGGTGAAAGGATTCAGTATACTCAATGTGAACATTGTCTGAAGTGGCGCAAATTGCCAGCATGTGCACTTCTTCCAGCAAAATGGACATGTTCATATAATTCATGGGATCCAGAAAGATCTTCTTGTTCAGCTGCACAAGAGCTTACAACAGAACAACTTGAGAATTTGTTACCCCCTTGTAATTCAGCAGTTTCCAAGAAAATGAAGGCCACTAAGCAGGATCCTGATCATGCTGAAGCTTTGGAGGGACTTGATACACTTGCAAATCTAGCAATCCTGGGGGAAGGGGAGGCTCTCCCATCTTCCCAGGCCACAACTAAACACCCACGACATAGACCCGGCTGCTCTTGCATTGTTTGCATCCAGCCGCCTAGTGGAAAGGGCCCCAAGCATAAGCAGACATGCACATGTAATGTCTGTCTAACGGTGAAGCGTCGTTTCCGTACCCTTATGTTACGGCGTGAGAAGAAACAGTCCGAAAAAGAAGCAGAAACAACACGTAAAAAGCAGCAGCAACAGAATTCCCAGCAACTGCCTTCATCTGAAATTTTGCTTGACGATGATTCCTTGCATAATAGTAATACGGGTGATAGTAGCCCAACCATGAACAAGGAGGGTAATGATGGTTCTGATGATGATCCTCACCGGATAAAATCATCCGTGTCTCCATTTAAAGGCCAAATTGATCTCAATATCCAGCCAGAGCGAGAAGAAGAATTGTCGCCTGGTTCAGATTCTGGTGGTGTGATGAAGCTGCTCCACGATGCTGCTGCTGAAATGTATCTCAAGCAGCAGCAGACTTTGTTGAACTCTGGTACTGGAGATTCATCAGGTAGTCAGTCACAGCAGGTAGGAGATGTTGTGAGGGAAGTGAAGCTTAGCAACGGTGTTATTCATGGTAGCAGTAGTCATAATGCTGATAAGGAACATGCCCAGTCTTTGTCTATGAACGTGTGA
Protein sequence

>MsaT006437.1

MALINNFLYSSQSSYVCSAFEEGRFCDIFHSNASGWRNCETCRKRIHCGCIVSSNTFVLLDPGGIECFTCAHKMLFCDFSLYLLSLSQPSNLPWPQSLSLTNRLSERYRDMSGKSWSQLAGSGPVPWKQAPSLFNSASSSDQPPDVHSLVDLSNNFDKIYGNERLPPSSLEKKNEDLSGISANWNVKLGSRDMLLMNGMRNEDKSGPCLNMCQQPHTLKEDSSSQPFGLSVPYSSPNERNGQIGVTGIHLQQTPPHLGKQFSGTMHLPLDSSGEAQVRNGRPRADARGRSQLLPRYWPRCTELELQQISVDSNSVITPLFQKTLSASDAGRIGRLVLPKKCAETYFPPISQPEGLPLKILDAKGKEWIFQFRFWPNNNSRMYVLEGVTPCIQSMQLQAGDTVTFSRLEPEGRLVMGFRKATNPLPSDQDNEANKTGNGFSAHEVELADPSSWSNVDKSGYIAKEALGSKSLISRKRKNNILGSKSKRLKIENEDIIELKITWQEAQGLLRPPPNHVPSIVVIEGFEFEEYEDAPVLGKPSIFTSDNMGERIQYTQCEHCLKWRKLPACALLPAKWTCSYNSWDPERSSCSAAQELTTEQLENLLPPCNSAVSKKMKATKQDPDHAEALEGLDTLANLAILGEGEALPSSQATTKHPRHRPGCSCIVCIQPPSGKGPKHKQTCTCNVCLTVKRRFRTLMLRREKKQSEKEAETTRKKQQQQNSQQLPSSEILLDDDSLHNSNTGDSSPTMNKEGNDGSDDDPHRIKSSVSPFKGQIDLNIQPEREEELSPGSDSGGVMKLLHDAAAEMYLKQQQTLLNSGTGDSSGSQSQQVGDVVREVKLSNGVIHGSSSHNADKEHAQSLSMNV*