AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006566


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006566 MtrunA17_Chr7g0231611 88.034 351 35 3 1 349 1 346 0.0 625
MsaG006566 MtrunA17_Chr5g0416851 88.319 351 34 3 1 349 1 346 0.0 615
MsaG006566 MtrunA17_Chr2g0282701 86.275 255 15 2 96 349 42 277 7.67e-148 418
MsaG006566 MtrunA17_Chr1g0182611 33.099 142 81 6 1 132 1 138 1.18e-15 77.8
MsaG006566 MtrunA17_Chr1g0182621 35.294 119 63 4 22 134 28 138 1.53e-14 74.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006566 AT1G58210.1 38.053 113 63 3 22 132 64 171 1.25e-17 82.4

Find 73 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCACTTTACGTAAAGAAAT+TGG 0.221596 2:-6941160 MsaT006566.1:CDS
CCTTGTGGTCACTTGATAAT+TGG 0.239096 2:+6941130 None:intergenic
GTGTGGTGGTGGAACGTATT+GGG 0.250160 2:+6942350 None:intergenic
GGTGGAACGTATTGGGGTTT+TGG 0.271264 2:+6942357 None:intergenic
TCCAATTTCTTTACGTAAAG+TGG 0.301497 2:+6941159 None:intergenic
GTGATTTATAATGGTGAAAA+TGG 0.304153 2:-6941483 MsaT006566.1:CDS
CAGTTGGATTTCCACACAAT+TGG 0.315477 2:-6941677 MsaT006566.1:CDS
GTGATTTCTAATGGTGAAAA+TGG 0.338731 2:-6941273 MsaT006566.1:CDS
GCCTCACATACATCTCAAAA+TGG 0.350427 2:-6941936 MsaT006566.1:CDS
GTTGGAGAGGGGTGTGGTGG+TGG 0.351038 2:+6942339 None:intergenic
TGTGGTGGTGGAACGTATTG+GGG 0.352785 2:+6942351 None:intergenic
CAAGTTGGCGTGATTTATAA+TGG 0.357302 2:-6941492 MsaT006566.1:CDS
TGCAAGGGTTTAGCTCTTGC+TGG 0.372413 2:-6942161 MsaT006566.1:CDS
GGTGTGGTGGTGGAACGTAT+TGG 0.373524 2:+6942349 None:intergenic
AATTATTCAGCTCATAGTTC+AGG 0.376079 2:-6941651 MsaT006566.1:CDS
GCTAAACCCTTGCATTGGTT+TGG 0.379957 2:+6942170 None:intergenic
AGCAGCTGATGAAACTTTGC+AGG 0.381501 2:-6941418 MsaT006566.1:CDS
AAGTCACATGATGCTTATAA+AGG 0.387819 2:-6941025 MsaT006566.1:CDS
TGAAACTTTGCAGGAAGATA+TGG 0.392140 2:-6941409 MsaT006566.1:CDS
TCTTGCTGGCATTGCTTCAA+TGG 0.402134 2:-6942147 MsaT006566.1:CDS
ATACCGGTAACTTGTGTAGT+AGG 0.407561 2:-6941074 MsaT006566.1:CDS
ACAACAATATTGCGAGTCTA+AGG 0.409478 2:-6941527 MsaT006566.1:CDS
CAGGTTGCCGTGATTTCTAA+TGG 0.414513 2:-6941282 MsaT006566.1:CDS
CACCCTACTACACAAGTTAC+CGG 0.426920 2:+6941071 None:intergenic
CCCATGAGCCAGGCAGTGTT+AGG 0.434002 2:-6941185 MsaT006566.1:CDS
TTTGAAGATTGAGTTGGAGA+GGG 0.438182 2:+6942327 None:intergenic
GGGTCTTGAGTTGTCTCAAC+AGG 0.439702 2:-6941361 MsaT006566.1:CDS
GAACACGTGGATAAAGTTAC+TGG 0.442596 2:-6941618 MsaT006566.1:CDS
AAACCCTTGCATTGGTTTGG+AGG 0.442882 2:+6942173 None:intergenic
ATCTTGAGTCTCTACAACAT+TGG 0.448912 2:+6941992 None:intergenic
GCAATGTTGTCATTACCTTC+AGG 0.449864 2:+6941384 None:intergenic
GAAGGTAATGACAACATTGC+GGG 0.450334 2:-6941381 MsaT006566.1:CDS
CACATGATGCTTATAAAGGA+TGG 0.459002 2:-6941021 MsaT006566.1:CDS
TTTAGATTTGAAGATTGAGT+TGG 0.459049 2:+6942321 None:intergenic
TCTTGAGTCTCTACAACATT+GGG 0.460912 2:+6941993 None:intergenic
CTTCTGTTAGAAGATCTACC+AGG 0.478887 2:-6940975 MsaT006566.1:CDS
ACCTAACACTGCCTGGCTCA+TGG 0.480484 2:+6941184 None:intergenic
GCAATGTTGTCATTACCTTC+CGG 0.481010 2:+6941327 None:intergenic
TGAGTTGGAGAGGGGTGTGG+TGG 0.489080 2:+6942336 None:intergenic
ATTTGAAGATTGAGTTGGAG+AGG 0.496033 2:+6942326 None:intergenic
TTATGTCGCCGTTTCTCAGT+TGG 0.496930 2:-6941693 MsaT006566.1:CDS
TGATACATTTCTTATCCTTG+TGG 0.504839 2:+6941115 None:intergenic
GTTGTCTCAACAGGAAGATA+TGG 0.506535 2:-6941352 MsaT006566.1:CDS
GATTGAGTTGGAGAGGGGTG+TGG 0.509260 2:+6942333 None:intergenic
TTGAAGATTGAGTTGGAGAG+GGG 0.510736 2:+6942328 None:intergenic
TCTTGATTTGTCTCAACCGC+AGG 0.523290 2:-6941301 MsaT006566.1:CDS
CCTAACACTGCCTGGCTCAT+GGG 0.526114 2:+6941185 None:intergenic
CAAGAGCTAAACCCTTGCAT+TGG 0.528805 2:+6942165 None:intergenic
TCAACAGGAAGATATGGAAC+CGG 0.540435 2:-6941346 MsaT006566.1:CDS
TTTGATCCTACCCATGAGCC+AGG 0.541769 2:-6941195 MsaT006566.1:CDS
AAGAAGAATGTAGATGATAC+CGG 0.549093 2:-6941090 MsaT006566.1:CDS
AGTGGAGACCTAACACTGCC+TGG 0.549411 2:+6941177 None:intergenic
GTGGAAATCCAACTGAGAAA+CGG 0.556021 2:+6941685 None:intergenic
ACATGATGCTTATAAAGGAT+GGG 0.566978 2:-6941020 MsaT006566.1:CDS
GGCATCACTGTAATGTTCCA+CGG 0.577101 2:-6941969 MsaT006566.1:CDS
TTATAAATCACGCCAACTTG+AGG 0.591187 2:+6941495 None:intergenic
TACCGGTAACTTGTGTAGTA+GGG 0.605302 2:-6941073 MsaT006566.1:CDS
GAAACCTCCAAACCAATGCA+AGG 0.605866 2:-6942177 MsaT006566.1:CDS
ACACTGCCTGGCTCATGGGT+AGG 0.608929 2:+6941189 None:intergenic
CCAATTATCAAGTGACCACA+AGG 0.618748 2:-6941130 MsaT006566.1:CDS
CAGGAAGATATGGAACCTGA+AGG 0.622434 2:-6941399 MsaT006566.1:CDS
AAACCTCCAAACCAATGCAA+GGG 0.637222 2:-6942176 MsaT006566.1:CDS
AGGTTGTCTCAACCTCAAGT+TGG 0.639538 2:-6941507 MsaT006566.1:CDS
GTTACCTTCAGCTTCCTGTG+AGG 0.642766 2:+6941548 None:intergenic
GAGACCTCACAGGAAGCTGA+AGG 0.643008 2:-6941552 MsaT006566.1:CDS
AACAGCTGATGAGACCTCAC+AGG 0.646895 2:-6941562 MsaT006566.1:CDS
AATGTTGTAGAGACTCAAGA+TGG 0.656225 2:-6941990 MsaT006566.1:CDS
CAGGAAGATATGGAACCGGA+AGG 0.662654 2:-6941342 MsaT006566.1:CDS
AGGAAATGAATGTGAACACG+TGG 0.663886 2:-6941631 MsaT006566.1:CDS
TTCTGTTAGAAGATCTACCA+GGG 0.673391 2:-6940974 MsaT006566.1:CDS
TTAGAAATCACGGCAACCTG+CGG 0.679371 2:+6941285 None:intergenic
TGAAGGTAATGACAACATTG+CGG 0.680073 2:-6941382 MsaT006566.1:CDS
GTGAGGCGTTAATCAAACCG+TGG 0.715788 2:+6941952 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 6940954 6942386 6940954 ID=MsaG006566
Chr2 mRNA 6940954 6942386 6940954 ID=MsaT006566.1;Parent=MsaG006566
Chr2 exon 6940954 6941715 6940954 ID=MsaT006566.1.exon4;Parent=MsaT006566.1
Chr2 CDS 6940954 6941715 6940954 ID=cds.MsaT006566.1;Parent=MsaT006566.1
Chr2 exon 6941923 6942061 6941923 ID=MsaT006566.1.exon3;Parent=MsaT006566.1
Chr2 CDS 6941923 6942061 6941923 ID=cds.MsaT006566.1;Parent=MsaT006566.1
Chr2 exon 6942146 6942225 6942146 ID=MsaT006566.1.exon2;Parent=MsaT006566.1
Chr2 CDS 6942146 6942225 6942146 ID=cds.MsaT006566.1;Parent=MsaT006566.1
Chr2 exon 6942318 6942386 6942318 ID=MsaT006566.1.exon1;Parent=MsaT006566.1
Chr2 CDS 6942318 6942386 6942318 ID=cds.MsaT006566.1;Parent=MsaT006566.1
Gene Sequence

>MsaT006566.1

ATGGCTTCCAAAACCCCAATACGTTCCACCACCACACCCCTCTCCAACTCAATCTTCAAATCTAAACACATTTTCTTACATGAATGGTGGCTTGTGAAACCTCCAAACCAATGCAAGGGTTTAGCTCTTGCTGGCATTGCTTCAATGGAGAGAGAAAGAATGTTTTTTTCTTCACTGATTGTAGAGAGACATGAACCCAATGTTGTAGAGACTCAAGATGGCATCACTGTAATGTTCCACGGTTTGATTAACGCCTCACATACATCTCAAAATGGTTTTTCTTCTGAGTTATGTCGCCGTTTCTCAGTTGGATTTCCACACAATTGGAAAAATTATTCAGCTCATAGTTCAGGAAATGAATGTGAACACGTGGATAAAGTTACTGGTTTTGACGACTCAAATACTTCTTCCCATAAAAAAACAGCTGATGAGACCTCACAGGAAGCTGAAGGTAACAACAATATTGCGAGTCTAAGGTTGTCTCAACCTCAAGTTGGCGTGATTTATAATGGTGAAAATGGATTCTCAAATGTTGACAACTCAAATGCTTCATCCCACAAAAAAGCAGCTGATGAAACTTTGCAGGAAGATATGGAACCTGAAGGTAATGACAACATTGCGGGTCTTGAGTTGTCTCAACAGGAAGATATGGAACCGGAAGGTAATGACAACATTGCGAGTCTTGATTTGTCTCAACCGCAGGTTGCCGTGATTTCTAATGGTGAAAATGGAGTCTCAAGTGTTGCTGCAGCTGAAAATAGTCAATCTTTGATGAGTGTATTTGAATTTGATCCTACCCATGAGCCAGGCAGTGTTAGGTCTCCACTTTACGTAAAGAAATTGGAGTTTGACCAATTATCAAGTGACCACAAGGATAAGAAATGTATCAAGAAGAAGAATGTAGATGATACCGGTAACTTGTGTAGTAGGGTGCTAACAAGAAGCATTGCCAAGAAAAGTCACATGATGCTTATAAAGGATGGGAAAGCTGAAGTAAAATGTTTCACTTCTGTTAGAAGATCTACCAGGGTACTAAATTATAAAAAATGA

Protein sequence

>MsaT006566.1

MASKTPIRSTTTPLSNSIFKSKHIFLHEWWLVKPPNQCKGLALAGIASMERERMFFSSLIVERHEPNVVETQDGITVMFHGLINASHTSQNGFSSELCRRFSVGFPHNWKNYSAHSSGNECEHVDKVTGFDDSNTSSHKKTADETSQEAEGNNNIASLRLSQPQVGVIYNGENGFSNVDNSNASSHKKAADETLQEDMEPEGNDNIAGLELSQQEDMEPEGNDNIASLDLSQPQVAVISNGENGVSSVAAAENSQSLMSVFEFDPTHEPGSVRSPLYVKKLEFDQLSSDHKDKKCIKKKNVDDTGNLCSRVLTRSIAKKSHMMLIKDGKAEVKCFTSVRRSTRVLNYKK*