AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006581

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006581 MtrunA17_Chr2g0282871 94.624 186 10 0 1 186 1 186 5.35e-134 377
MsaG006581 MtrunA17_Chr8g0367761 67.662 201 50 3 1 186 1 201 3.96e-98 284
MsaG006581 MtrunA17_Chr4g0046431 67.647 204 45 2 1 186 1 201 5.72e-98 286
MsaG006581 MtrunA17_Chr2g0324961 66.845 187 56 2 1 186 1 182 3.17e-88 260
MsaG006581 MtrunA17_Chr4g0040541 69.101 178 52 3 12 186 5 182 2.73e-86 255
MsaG006581 MtrunA17_Chr8g0389661 58.201 189 65 5 8 186 3 187 9.85e-66 209
MsaG006581 MtrunA17_Chr5g0422431 56.875 160 66 2 27 186 34 190 7.42e-55 179
MsaG006581 MtrunA17_Chr1g0198751 50.802 187 87 3 2 186 7 190 2.60e-54 179
MsaG006581 MtrunA17_Chr7g0227071 54.088 159 66 3 31 186 35 189 1.23e-51 172
MsaG006581 MtrunA17_Chr1g0198741 56.962 79 34 0 108 186 7 85 1.15e-21 89.4
MsaG006581 MtrunA17_Chr7g0217711 30.263 152 89 4 36 186 2 137 8.93e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006581 AT4G28500.1 62.176 193 66 3 1 186 1 193 1.28e-83 249
MsaG006581 AT1G28470.1 60.000 210 60 4 1 186 1 210 3.36e-83 249
MsaG006581 AT4G29230.3 58.333 192 65 3 6 186 2 189 1.62e-69 218
MsaG006581 AT4G29230.1 58.333 192 65 3 6 186 2 189 6.43e-69 218
MsaG006581 AT4G29230.2 57.292 192 66 3 6 186 2 188 8.39e-68 215
MsaG006581 AT5G56620.2 55.307 179 66 4 19 186 12 187 3.86e-58 187
MsaG006581 AT5G56620.1 55.307 179 66 4 19 186 12 187 4.07e-58 187
MsaG006581 AT5G56620.3 57.831 166 56 4 32 186 25 187 4.10e-58 187
MsaG006581 AT1G25580.1 53.750 160 66 3 27 186 34 185 2.33e-51 171
MsaG006581 AT5G14490.2 45.198 177 91 3 11 186 18 189 2.25e-42 143
MsaG006581 AT5G14490.1 49.367 158 77 2 30 186 37 192 6.26e-42 144
MsaG006581 AT5G14490.4 49.367 158 77 2 30 186 34 189 7.04e-42 144
MsaG006581 AT5G14490.3 49.367 158 77 2 30 186 37 192 9.46e-42 144
MsaG006581 AT3G01600.1 43.503 177 94 3 11 186 18 189 3.05e-39 137

Find 32 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACAAGATGGCACAAAAC+TGG 0.293534 2:+7121707 MsaT006581.1:CDS
TCTAGTACTTCTACATCATT+TGG 0.314489 2:-7120118 None:intergenic
CATCACCAGTTAGTCTTCTC+TGG 0.335946 2:-7121818 None:intergenic
CCAACGCTTCAAGGAGATAA+TGG 0.355602 2:+7120205 MsaT006581.1:CDS
TGTAGAAGTACTAGAGCATT+TGG 0.390413 2:+7120126 MsaT006581.1:CDS
GGGTGGAGTTAATGAATTGC+CGG 0.417191 2:+7120069 MsaT006581.1:intron
GAAAACCAGAGAAGACTAAC+TGG 0.430346 2:+7121813 MsaT006581.1:CDS
CTAGTACTTCTACATCATTT+GGG 0.458512 2:-7120117 None:intergenic
GAGTTCATACCAACGCTTCA+AGG 0.459148 2:+7120196 MsaT006581.1:CDS
CCATTATCTCCTTGAAGCGT+TGG 0.461132 2:-7120205 None:intergenic
GCCGCATGAGGGGCATTCAA+TGG 0.476343 2:-7119775 None:intergenic
GAATTGCCGGGGTTACTAGC+TGG 0.479303 2:+7120082 MsaT006581.1:CDS
TACACCAACTATTGCAAAGA+AGG 0.482059 2:+7121791 MsaT006581.1:CDS
CCTTCAAAAGCATACACAAC+AGG 0.484981 2:+7121641 MsaT006581.1:CDS
GGTGGAGTTAATGAATTGCC+GGG 0.501114 2:+7120070 MsaT006581.1:CDS
TTCAATATTATGGCCGCATG+AGG 0.506854 2:-7119787 None:intergenic
AAATCCCTTCACAACACCAT+TGG 0.513981 2:-7121750 None:intergenic
TCAATATTATGGCCGCATGA+GGG 0.518397 2:-7119786 None:intergenic
GTTGCAGGAGTTAGCAAAGA+TGG 0.520564 2:+7121593 MsaT006581.1:intron
AAGAAGGAATTGAAACAAGA+TGG 0.540463 2:+7121696 MsaT006581.1:CDS
AAACAAGATGGCACAAAACT+GGG 0.551473 2:+7121708 MsaT006581.1:CDS
AAGGTTCACACTGATGAAGA+AGG 0.564034 2:+7121680 MsaT006581.1:CDS
CAATATTATGGCCGCATGAG+GGG 0.567135 2:-7119785 None:intergenic
AGGAACAAGGAAAAGAAGAA+AGG 0.568088 2:+7121661 MsaT006581.1:CDS
ACACACCCAGCACAACTACC+AGG 0.587266 2:+7120238 MsaT006581.1:CDS
GGTATGAACTCATCAATAAG+TGG 0.587548 2:-7120184 None:intergenic
TTCACTCCAGCTAGTAACCC+CGG 0.611670 2:-7120088 None:intergenic
ACCATTGAATGCCCCTCATG+CGG 0.616642 2:+7119774 MsaT006581.1:CDS
AGAAGTACTAGAGCATTTGG+AGG 0.620573 2:+7120129 MsaT006581.1:CDS
AAAGATTAAGAACTATGGCA+TGG 0.622011 2:+7119676 None:intergenic
AAGCATACACAACAGGAACA+AGG 0.626885 2:+7121648 MsaT006581.1:CDS
GTGGAGTTAATGAATTGCCG+GGG 0.680068 2:+7120071 MsaT006581.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 7119690 7121838 7119690 ID=MsaG006581
Chr2 mRNA 7119690 7121838 7119690 ID=MsaT006581.1;Parent=MsaG006581
Chr2 exon 7119690 7119821 7119690 ID=MsaT006581.1.exon1;Parent=MsaT006581.1
Chr2 CDS 7119690 7119821 7119690 ID=cds.MsaT006581.1;Parent=MsaT006581.1
Chr2 exon 7120070 7120259 7120070 ID=MsaT006581.1.exon2;Parent=MsaT006581.1
Chr2 CDS 7120070 7120259 7120070 ID=cds.MsaT006581.1;Parent=MsaT006581.1
Chr2 exon 7121600 7121838 7121600 ID=MsaT006581.1.exon3;Parent=MsaT006581.1
Chr2 CDS 7121600 7121838 7121600 ID=cds.MsaT006581.1;Parent=MsaT006581.1
Gene Sequence

>MsaT006581.1

ATGGCATGGTGCAATGATAAAGATAACAACAACTCTGATGTCACTAATAACATAAAGCTCAATGAAGAAATTGATCAAAATCAAACCATTGAATGCCCCTCATGCGGCCATAATATTGAAGTAAAAAATCAGGGTGGAGTTAATGAATTGCCGGGGTTACTAGCTGGAGTGAAATTTGACCCAAATGATGTAGAAGTACTAGAGCATTTGGAGGCAAAAGTGATGTCTGATGTTCGCAAGATTCATCCACTTATTGATGAGTTCATACCAACGCTTCAAGGAGATAATGGAATTTGTTATACACACCCAGCACAACTACCAGGAGTTAGCAAAGATGGTCAAATTCGCCACTTTTTTCATAGGCCTTCAAAAGCATACACAACAGGAACAAGGAAAAGAAGAAAGGTTCACACTGATGAAGAAGGAATTGAAACAAGATGGCACAAAACTGGGAAAACTAGAGCAATTTTTTCCAATGGTGTTGTGAAGGGATTTAAGAAGATACTTGTACTCTACACCAACTATTGCAAAGAAGGAAAACCAGAGAAGACTAACTGGTGA
Protein sequence

>MsaT006581.1

MAWCNDKDNNNSDVTNNIKLNEEIDQNQTIECPSCGHNIEVKNQGGVNELPGLLAGVKFDPNDVEVLEHLEAKVMSDVRKIHPLIDEFIPTLQGDNGICYTHPAQLPGVSKDGQIRHFFHRPSKAYTTGTRKRRKVHTDEEGIETRWHKTGKTRAIFSNGVVKGFKKILVLYTNYCKEGKPEKTNW*