AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006673

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006673 MtrunA17_Chr2g0283951 88.083 386 34 3 1 382 1 378 0.0 663
MsaG006673 MtrunA17_Chr8g0379121 45.047 424 150 11 22 382 25 428 2.33e-92 283
MsaG006673 MtrunA17_Chr2g0320231 55.191 183 62 6 32 198 30 208 2.65e-46 162
MsaG006673 MtrunA17_Chr7g0243081 54.815 135 50 3 29 161 10 135 2.81e-36 135
MsaG006673 MtrunA17_Chr1g0162931 63.095 84 26 1 85 168 6 84 1.43e-29 115
MsaG006673 MtrunA17_Chr1g0210911 64.198 81 25 2 79 159 7 83 3.80e-28 112
MsaG006673 MtrunA17_Chr2g0288601 75.000 60 15 0 89 148 63 122 2.80e-26 102
MsaG006673 MtrunA17_Chr3g0095611 55.405 74 33 0 80 153 2 75 7.59e-23 95.5
MsaG006673 MtrunA17_Chr1g0154261 57.500 80 29 2 89 165 120 197 1.34e-19 88.2
MsaG006673 MtrunA17_Chr1g0190301 56.579 76 32 1 78 153 157 231 3.07e-19 88.6
MsaG006673 MtrunA17_Chr7g0261911 67.857 56 18 0 98 153 263 318 3.53e-19 89.0
MsaG006673 MtrunA17_Chr6g0479431 65.517 58 20 0 96 153 201 258 4.40e-19 87.8
MsaG006673 MtrunA17_Chr7g0274471 66.071 56 19 0 98 153 219 274 1.13e-18 87.0
MsaG006673 MtrunA17_Chr8g0366281 50.602 83 32 1 96 178 172 245 2.02e-18 85.9
MsaG006673 MtrunA17_Chr5g0410741 58.571 70 28 1 96 164 218 287 2.19e-18 86.3
MsaG006673 MtrunA17_Chr3g0109981 65.517 58 20 0 96 153 273 330 3.60e-18 85.9
MsaG006673 MtrunA17_Chr7g0258551 54.839 62 28 0 93 154 208 269 2.42e-16 79.7
MsaG006673 MtrunA17_Chr6g0461641 60.377 53 21 0 98 150 43 95 3.86e-16 79.3
MsaG006673 MtrunA17_Chr1g0175091 51.250 80 35 2 74 153 239 314 6.44e-16 79.0
MsaG006673 MtrunA17_Chr5g0417521 50.769 65 30 1 91 153 7 71 8.04e-16 78.2
MsaG006673 MtrunA17_Chr5g0409731 53.125 64 30 0 87 150 15 78 8.76e-16 77.0
MsaG006673 MtrunA17_Chr2g0321461 57.143 56 24 0 98 153 46 101 1.00e-15 78.2
MsaG006673 MtrunA17_Chr2g0291021 65.455 55 19 0 99 153 51 105 1.88e-15 76.6
MsaG006673 MtrunA17_Chr7g0254981 61.818 55 21 0 99 153 52 106 2.73e-15 75.5
MsaG006673 MtrunA17_Chr1g0175081 54.545 55 25 0 99 153 24 78 4.15e-15 70.9
MsaG006673 MtrunA17_Chr1g0197171 57.143 56 24 0 98 153 45 100 1.33e-14 73.6
MsaG006673 MtrunA17_Chr2g0290991 57.143 56 24 0 98 153 40 95 1.70e-14 73.6
MsaG006673 MtrunA17_Chr8g0356501 55.357 56 25 0 98 153 38 93 1.77e-14 73.9
MsaG006673 MtrunA17_Chr2g0310011 55.357 56 25 0 98 153 36 91 3.30e-14 73.2
MsaG006673 MtrunA17_Chr1g0175071 60.377 53 21 0 99 151 179 231 3.97e-14 72.8
MsaG006673 MtrunA17_Chr7g0242521 59.322 59 21 1 99 154 15 73 5.55e-14 70.9
MsaG006673 MtrunA17_Chr4g0042451 53.571 56 26 0 98 153 18 73 5.60e-14 72.4
MsaG006673 MtrunA17_Chr4g0009901 46.667 75 36 2 76 150 150 220 6.03e-14 71.6
MsaG006673 MtrunA17_Chr4g0009991 37.234 94 55 2 55 148 28 117 1.41e-12 64.3
MsaG006673 MtrunA17_Chr4g0009981 41.758 91 44 3 65 150 82 168 8.77e-12 65.1
MsaG006673 MtrunA17_Chr7g0258531 56.604 53 23 0 99 151 215 267 9.09e-12 65.9
MsaG006673 MtrunA17_Chr2g0304841 52.727 55 25 1 96 150 212 265 2.86e-11 65.1
MsaG006673 MtrunA17_Chr4g0067981 54.545 55 24 1 96 150 214 267 5.59e-11 64.3
MsaG006673 MtrunA17_Chr1g0161431 33.898 118 49 3 61 150 136 252 7.37e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006673 AT2G38300.1 68.750 80 25 0 83 162 40 119 8.83e-33 125
MsaG006673 AT2G40260.1 77.333 75 17 0 82 156 67 141 9.91e-31 121
MsaG006673 AT2G42660.1 50.000 130 39 2 26 155 5 108 5.28e-30 116
MsaG006673 AT4G04605.1 62.353 85 32 0 89 173 6 90 4.73e-29 112
MsaG006673 AT1G14600.1 61.446 83 32 0 77 159 3 85 2.18e-28 112
MsaG006673 AT2G02060.2 60.976 82 28 1 74 151 2 83 3.90e-26 105
MsaG006673 AT2G02060.1 60.976 82 28 1 74 151 2 83 3.90e-26 105
MsaG006673 AT4G04580.1 50.617 81 40 0 89 169 6 86 3.41e-20 87.0
MsaG006673 AT5G42630.2 63.492 63 23 0 96 158 104 166 1.86e-19 86.7
MsaG006673 AT5G42630.1 63.492 63 23 0 96 158 104 166 2.09e-19 87.8
MsaG006673 AT4G04555.1 56.944 72 30 1 86 156 2 73 2.21e-19 83.2
MsaG006673 AT4G17695.1 57.534 73 26 1 96 163 163 235 3.40e-18 85.1
MsaG006673 AT1G32240.2 65.517 58 20 0 96 153 212 269 3.65e-18 85.5
MsaG006673 AT1G32240.1 65.517 58 20 0 96 153 212 269 4.43e-18 85.5
MsaG006673 AT5G16560.1 65.517 58 20 0 96 153 218 275 4.51e-18 85.5
MsaG006673 AT4G28610.1 65.455 55 19 0 99 153 227 281 1.84e-17 84.0
MsaG006673 AT5G29000.4 38.400 125 70 2 29 153 170 287 2.60e-17 82.4
MsaG006673 AT3G04450.3 61.818 55 21 0 99 153 240 294 2.83e-17 82.0
MsaG006673 AT5G29000.1 65.455 55 19 0 99 153 190 244 2.94e-17 82.8
MsaG006673 AT5G29000.3 65.455 55 19 0 99 153 190 244 2.94e-17 82.8
MsaG006673 AT3G04450.4 61.818 55 21 0 99 153 200 254 3.39e-17 82.8
MsaG006673 AT3G04450.2 61.818 55 21 0 99 153 200 254 3.39e-17 82.8
MsaG006673 AT3G04450.1 61.818 55 21 0 99 153 240 294 3.82e-17 83.2
MsaG006673 AT5G29000.2 60.656 61 24 0 93 153 227 287 3.97e-17 82.8
MsaG006673 AT3G13040.3 67.273 55 18 0 99 153 243 297 2.76e-16 80.5
MsaG006673 AT3G13040.1 67.273 55 18 0 99 153 243 297 2.76e-16 80.5
MsaG006673 AT3G13040.2 67.273 55 18 0 99 153 243 297 2.76e-16 80.5
MsaG006673 AT5G45580.1 56.897 58 25 0 93 150 3 60 3.77e-16 78.2
MsaG006673 AT5G45580.2 56.897 58 25 0 93 150 19 76 4.78e-16 78.2
MsaG006673 AT3G12730.1 38.739 111 59 3 98 204 24 129 1.31e-15 75.9
MsaG006673 AT2G01060.1 65.455 55 19 0 99 153 17 71 1.34e-15 77.0
MsaG006673 AT1G79430.2 40.777 103 51 3 98 198 35 129 1.66e-15 77.4
MsaG006673 AT3G04030.1 53.571 56 26 0 98 153 46 101 2.33e-15 77.4
MsaG006673 AT3G04030.3 53.571 56 26 0 98 153 46 101 2.63e-15 77.4
MsaG006673 AT2G06020.1 32.065 184 108 6 54 221 39 221 4.47e-15 75.5
MsaG006673 AT2G20400.2 63.636 55 20 0 99 153 233 287 5.07e-15 76.3
MsaG006673 AT2G20400.1 63.636 55 20 0 99 153 233 287 5.19e-15 76.3
MsaG006673 AT5G18240.2 53.571 56 26 0 98 153 46 101 7.46e-15 75.9
MsaG006673 AT5G18240.3 53.571 56 26 0 98 153 46 101 7.46e-15 75.9
MsaG006673 AT5G18240.5 53.571 56 26 0 98 153 46 101 8.41e-15 75.9
MsaG006673 AT5G18240.1 53.571 56 26 0 98 153 46 101 8.57e-15 75.9
MsaG006673 AT5G18240.4 53.571 56 26 0 98 153 46 101 8.57e-15 75.9
MsaG006673 AT4G13640.1 56.604 53 23 0 98 150 38 90 1.50e-14 73.9
MsaG006673 AT4G13640.2 56.604 53 23 0 98 150 38 90 1.54e-14 73.9
MsaG006673 AT3G24120.1 56.604 53 23 0 98 150 42 94 1.72e-14 73.9
MsaG006673 AT3G24120.2 56.604 53 23 0 98 150 42 94 1.81e-14 73.9
MsaG006673 AT5G06800.3 56.364 55 24 0 99 153 194 248 3.44e-14 72.8
MsaG006673 AT1G69580.1 51.667 60 29 0 98 157 35 94 5.84e-14 72.8
MsaG006673 AT1G69580.2 51.667 60 29 0 98 157 35 94 6.22e-14 72.8
MsaG006673 AT5G06800.2 50.769 65 31 1 99 162 194 258 7.96e-14 72.8
MsaG006673 AT5G06800.1 56.364 55 24 0 99 153 194 248 1.01e-13 72.4
MsaG006673 AT3G04030.2 51.786 56 26 1 98 153 46 100 6.70e-13 70.1
MsaG006673 AT3G62670.1 53.333 60 24 1 95 150 209 268 2.13e-12 68.6
MsaG006673 AT5G59570.2 55.357 56 24 1 95 150 139 193 9.12e-12 65.9
MsaG006673 AT5G59570.1 55.357 56 24 1 95 150 139 193 9.12e-12 65.9
MsaG006673 AT4G37180.1 51.667 60 29 0 91 150 204 263 4.58e-11 64.3
MsaG006673 AT3G46640.2 54.545 55 24 1 96 150 143 196 4.94e-11 63.9
MsaG006673 AT3G46640.1 54.545 55 24 1 96 150 143 196 4.94e-11 63.9
MsaG006673 AT1G25550.1 38.596 114 57 2 99 212 211 311 5.32e-11 63.9
MsaG006673 AT4G37180.2 51.667 60 29 0 91 150 211 270 5.56e-11 63.9
MsaG006673 AT3G46640.3 54.545 55 24 1 96 150 143 196 5.81e-11 63.5

Find 69 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTCAAGTATTGTTGCTTCA+TGG 0.216171 2:+8284512 None:intergenic
GGAAGAGTTGCAAGGTTCTT+TGG 0.287029 2:+8281217 None:intergenic
AGCAAGAAACTTGACGAGTT+TGG 0.326402 2:-8283787 MsaT006673.1:CDS
CTTGTGTTGACCTCTGTGTT+TGG 0.327855 2:+8282216 None:intergenic
CTCAAACCTAGTTGCAAATC+AGG 0.328852 2:+8281006 None:intergenic
ATGATCAATCTTACCATCAT+TGG 0.345938 2:+8280969 None:intergenic
TTCCATTCAACTTTCTCAAT+AGG 0.352795 2:+8281287 None:intergenic
AGAAGGAAGGGCATGGCTGC+AGG 0.377318 2:+8282075 None:intergenic
GATGAAAGTAATACTTCATC+TGG 0.379913 2:-8284598 MsaT006673.1:CDS
TCATGTCAAGTCTCACTTGC+AGG 0.383436 2:-8284153 MsaT006673.1:intron
AATGTTACCTTGATCCGAAA+TGG 0.391625 2:+8281167 None:intergenic
TAGCTTAGTGCTAATTTCCT+TGG 0.409360 2:+8280936 None:intergenic
TGAAGCCCCATTTCGGATCA+AGG 0.411464 2:-8281174 MsaT006673.1:intron
ATGTTACCTTGATCCGAAAT+GGG 0.413170 2:+8281168 None:intergenic
CAACTCATCATTATGATTCA+AGG 0.416320 2:-8282022 MsaT006673.1:intron
TCTGTGAATAATTACGAGAT+AGG 0.420426 2:+8282051 None:intergenic
CCAATGTAACTTCTAAAACT+TGG 0.423539 2:-8281316 MsaT006673.1:CDS
CCACCTTAACCTAGGCATCT+TGG 0.426029 2:+8284378 None:intergenic
CATTAGCTGAAGCACCAACT+TGG 0.437054 2:+8284199 None:intergenic
CATGAACAATATTCATCAAC+CGG 0.438171 2:-8281357 MsaT006673.1:CDS
AGAAATTGGGTTGGTGTCAC+GGG 0.446810 2:+8281410 None:intergenic
TTCTTACCTGATTTGCAACT+AGG 0.452743 2:-8281012 MsaT006673.1:CDS
ATATTGTTCATGATTTCAAA+TGG 0.455511 2:+8281368 None:intergenic
AAGAAATTGGGTTGGTGTCA+CGG 0.469951 2:+8281409 None:intergenic
CATGCTGTTGATAGACTTGG+TGG 0.470120 2:-8284328 MsaT006673.1:CDS
TACTTTCATCAACAAGCTGC+AGG 0.473446 2:+8284611 None:intergenic
GATCCAAGATGCCTAGGTTA+AGG 0.477461 2:-8284381 MsaT006673.1:CDS
TTCATCAACCGGAATTCAAA+AGG 0.482722 2:-8281346 MsaT006673.1:CDS
CTAGGTTTGAGTCACACATA+TGG 0.486414 2:-8280994 MsaT006673.1:CDS
CAACATTTCAAGATGGGTAA+TGG 0.492659 2:-8282161 MsaT006673.1:CDS
AATGAGAAGGAAAATATTGA+AGG 0.500567 2:-8284463 MsaT006673.1:CDS
TTGCATGCTGTTGATAGACT+TGG 0.505073 2:-8284331 MsaT006673.1:CDS
GGGCATGGCTGCAGGAGAGT+TGG 0.508149 2:+8282083 None:intergenic
CACACATATGGAACCAATGA+TGG 0.515353 2:-8280982 MsaT006673.1:CDS
AACCTATTGAGAAAGTTGAA+TGG 0.520109 2:-8281289 MsaT006673.1:CDS
TCTAAGTCAACATTTCAAGA+TGG 0.523487 2:-8282168 MsaT006673.1:CDS
CTAAGTCAACATTTCAAGAT+GGG 0.525690 2:-8282167 MsaT006673.1:CDS
TTCTCTTCAAGAAATTGGGT+TGG 0.528659 2:+8281401 None:intergenic
GAAGGAAATAATGAAAGAAG+AGG 0.529416 2:-8284424 MsaT006673.1:CDS
AGCTTCTTCATTCAAATCAA+AGG 0.535953 2:+8284555 None:intergenic
ATTAGCTGAAGCACCAACTT+GGG 0.542418 2:+8284200 None:intergenic
CTGTGAATAATTACGAGATA+GGG 0.547778 2:+8282052 None:intergenic
GCTACAAACATGTCAGAAGA+AGG 0.551964 2:-8284670 None:intergenic
AAAATGTAGCAATAAACTTG+TGG 0.560518 2:+8281196 None:intergenic
GGTAATTGTAGATCAAGAGA+AGG 0.560724 2:-8284442 MsaT006673.1:CDS
ATGTGAGATCCAAGATGCCT+AGG 0.566870 2:-8284387 MsaT006673.1:CDS
GAATGAGAATGAGAATGAGA+AGG 0.569100 2:-8284476 MsaT006673.1:CDS
TGCAGGAGCAACACCCAAGT+TGG 0.569639 2:-8284213 MsaT006673.1:intron
AAATAATGAAAGAAGAGGCA+AGG 0.577829 2:-8284419 MsaT006673.1:CDS
CCAAGATGCCTAGGTTAAGG+TGG 0.584488 2:-8284378 MsaT006673.1:CDS
ATAATTACGAGATAGGGAGA+AGG 0.593369 2:+8282058 None:intergenic
CAGAGGTCAACACAAGAAGT+TGG 0.594613 2:-8282209 MsaT006673.1:CDS
TGATCTCTAGGCACCAACAT+TGG 0.600420 2:-8281463 MsaT006673.1:intron
TACGAGATAGGGAGAAGGAA+GGG 0.601154 2:+8282063 None:intergenic
AAACTTGTGGAAGAGTTGCA+AGG 0.606173 2:+8281209 None:intergenic
CATTTCAAGATGGGTAATGG+TGG 0.608538 2:-8282158 MsaT006673.1:CDS
TGATCATTGCAGAGAGACCA+AGG 0.608794 2:-8280953 MsaT006673.1:CDS
TAACAATTATATTCAGAGTG+AGG 0.608803 2:-8284635 MsaT006673.1:CDS
TGTTACCTTGATCCGAAATG+GGG 0.609773 2:+8281169 None:intergenic
GATGATTGAAGTGCCAATGT+TGG 0.609822 2:+8281450 None:intergenic
AAACTTGACGAGTTTGGACA+AGG 0.614003 2:-8283781 MsaT006673.1:intron
TTACGAGATAGGGAGAAGGA+AGG 0.615300 2:+8282062 None:intergenic
TCAGGAGTCCACCTTAACCT+AGG 0.637415 2:+8284370 None:intergenic
CCATGAAGCAACAATACTTG+AGG 0.647167 2:-8284512 MsaT006673.1:CDS
GATAGGGAGAAGGAAGGGCA+TGG 0.651977 2:+8282068 None:intergenic
GTCACGGGTGCAAATCTGAA+AGG 0.667252 2:+8281425 None:intergenic
CTTCAGCTAATGAATGTGAG+AGG 0.670692 2:-8284188 MsaT006673.1:CDS
AGGAGAGTTGGATAATACTG+AGG 0.696332 2:+8282095 None:intergenic
CAGTGCTATGCCAAACACAG+AGG 0.723353 2:-8282226 MsaT006673.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 8280924 8284683 8280924 ID=MsaG006673
Chr2 mRNA 8280924 8284683 8280924 ID=MsaT006673.1;Parent=MsaG006673
Chr2 exon 8280924 8281070 8280924 ID=MsaT006673.1.exon6;Parent=MsaT006673.1
Chr2 CDS 8280924 8281070 8280924 ID=cds.MsaT006673.1;Parent=MsaT006673.1
Chr2 exon 8281175 8281475 8281175 ID=MsaT006673.1.exon5;Parent=MsaT006673.1
Chr2 CDS 8281175 8281475 8281175 ID=cds.MsaT006673.1;Parent=MsaT006673.1
Chr2 exon 8282023 8282245 8282023 ID=MsaT006673.1.exon4;Parent=MsaT006673.1
Chr2 CDS 8282023 8282245 8282023 ID=cds.MsaT006673.1;Parent=MsaT006673.1
Chr2 exon 8283782 8283818 8283782 ID=MsaT006673.1.exon3;Parent=MsaT006673.1
Chr2 CDS 8283782 8283818 8283782 ID=cds.MsaT006673.1;Parent=MsaT006673.1
Chr2 exon 8284154 8284230 8284154 ID=MsaT006673.1.exon2;Parent=MsaT006673.1
Chr2 CDS 8284154 8284230 8284154 ID=cds.MsaT006673.1;Parent=MsaT006673.1
Chr2 exon 8284320 8284683 8284320 ID=MsaT006673.1.exon1;Parent=MsaT006673.1
Chr2 CDS 8284320 8284683 8284320 ID=cds.MsaT006673.1;Parent=MsaT006673.1
Gene Sequence

>MsaT006673.1

ATGTCAGAAGAAGGATATGAGATTCGTAACAATTATATTCAGAGTGAGGACCTGCAGCTTGTTGATGAAAGTAATACTTCATCTGGTTCTTCTAAAAAATGGTCTTCCTTTGATTTGAATGAAGAAGCTACAAGTGAAGATAATAACAACCATGAAGCAACAATACTTGAGGAGAATGAGAACGAGAATGAGAATGAGAATGAGAAGGAAAATATTGAAGGTAATTGTAGATCAAGAGAAGGAAATAATGAAAGAAGAGGCAAGGTTAGACATTATGTGAGATCCAAGATGCCTAGGTTAAGGTGGACTCCTGAGCTTCATCATTCTTTTTTGCATGCTGTTGATAGACTTGGTGGTCTAGAAAGAGCAACACCCAAGTTGGTGCTTCAGCTAATGAATGTGAGAGGACTCAACATTGCTCATGTCAAGTCTCACTTGCAGATGTATCGAAGCAAGAAACTTGACGAGTTTGGACAAGTGCTATGCCAAACACAGAGGTCAACACAAGAAGTTGGTAGATCTCAGAAACTCGATCTAAGTCAACATTTCAAGATGGGTAATGGTGGTATTATTCTATCAAGTAGTTACAATGAGCAGCATCATAATCCTCAGTATTATCCAACTCTCCTGCAGCCATGCCCTTCCTTCTCCCTATCTCGTAATTATTCACAGACAAACTCAACTCATCATTATGATTCAAGGCACCAACATTGGCACTTCAATCATCAACCTTTCAGATTTGCACCCGTGACACCAACCCAATTTCTTGAAGAGAAAAATTGGTCTCCATTTGAAATCATGAACAATATTCATCAACCGGAATTCAAAAGGCTACTTGCCAATGTAACTTCTAAAACTTGGTCACAACCTATTGAGAAAGTTGAATGGAACAACACAAGTAGTAGTATTACAAATAATACAGAATATCTATTGAAATCCAAAGAACCTTGCAACTCTTCCACAAGTTTATTGCTACATTTTGAAGCCCCATTTCGGATCAAGTTGAATCAAGAGAAAGTACATAAAGACGAGAAAAGATTCTTACCTGATTTGCAACTAGGTTTGAGTCACACATATGGAACCAATGATGGTAAGATTGATCATTGCAGAGAGACCAAGGAAATTAGCACTAAGCTATCACTTTCTTAG
Protein sequence

>MsaT006673.1

MSEEGYEIRNNYIQSEDLQLVDESNTSSGSSKKWSSFDLNEEATSEDNNNHEATILEENENENENENEKENIEGNCRSREGNNERRGKVRHYVRSKMPRLRWTPELHHSFLHAVDRLGGLERATPKLVLQLMNVRGLNIAHVKSHLQMYRSKKLDEFGQVLCQTQRSTQEVGRSQKLDLSQHFKMGNGGIILSSSYNEQHHNPQYYPTLLQPCPSFSLSRNYSQTNSTHHYDSRHQHWHFNHQPFRFAPVTPTQFLEEKNWSPFEIMNNIHQPEFKRLLANVTSKTWSQPIEKVEWNNTSSSITNNTEYLLKSKEPCNSSTSLLLHFEAPFRIKLNQEKVHKDEKRFLPDLQLGLSHTYGTNDGKIDHCRETKEISTKLSLS*