AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006713


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006713 MtrunA17_Chr2g0284471 85.263 190 19 4 12 197 133 317 1.83e-98 288
MsaG006713 MtrunA17_Chr8g0379681 51.724 145 56 3 57 191 182 322 1.34e-39 138
MsaG006713 MtrunA17_Chr7g0244571 37.838 148 81 4 46 189 203 343 1.80e-23 95.9
MsaG006713 MtrunA17_Chr4g0013081 37.576 165 92 4 35 197 227 382 2.96e-22 93.2
MsaG006713 MtrunA17_Chr5g0432531 33.742 163 79 6 33 185 103 246 1.54e-20 86.7
MsaG006713 MtrunA17_Chr8g0390531 48.529 68 35 0 125 192 258 325 1.46e-15 73.9
MsaG006713 MtrunA17_Chr4g0025901 27.907 129 84 1 66 185 31 159 2.54e-12 62.8
MsaG006713 MtrunA17_Chr4g0075391 40.678 59 35 0 127 185 83 141 5.55e-12 61.2
MsaG006713 MtrunA17_Chr1g0177881 42.857 63 36 0 128 190 105 167 3.37e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006713 AT4G18830.1 42.038 157 73 5 30 185 203 342 2.99e-32 119
MsaG006713 AT1G06920.1 63.636 66 22 1 124 189 242 305 8.75e-23 94.0
MsaG006713 AT2G30400.1 65.152 66 21 1 124 189 251 314 2.08e-22 92.8
MsaG006713 AT5G58360.1 44.578 83 44 1 107 189 206 286 4.61e-18 80.9
MsaG006713 AT5G01840.1 53.846 65 28 1 129 193 202 264 6.82e-18 80.1
MsaG006713 AT5G19650.1 49.180 61 31 0 125 185 154 214 3.70e-15 71.6
MsaG006713 AT2G18500.1 41.176 68 38 1 124 191 225 290 8.99e-13 66.2
MsaG006713 AT4G04025.1 42.254 71 41 0 115 185 103 173 1.76e-11 60.8
MsaG006713 AT3G52525.1 46.667 60 32 0 126 185 67 126 2.17e-11 60.5

Find 28 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTATTTACTAGTTAATC+TGG 0.300711 2:+8756850 MsaT006713.1:CDS
TTCACCCTTGAAACTTTCTT+TGG 0.300747 2:-8755990 None:intergenic
ATTTACTAGTTAATCTGGTT+TGG 0.305585 2:+8756855 MsaT006713.1:CDS
TAATTATGAGAAGCATAGTT+TGG 0.331902 2:+8755917 MsaT006713.1:CDS
TTTACTAGTTAATCTGGTTT+GGG 0.333093 2:+8756856 MsaT006713.1:CDS
GAAAATATATTCACCAAGAT+TGG 0.341908 2:+8756010 MsaT006713.1:CDS
ACAGCAAAACTATCTAATTC+TGG 0.358668 2:-8756137 None:intergenic
GATTAGTAAGGCAGAAGAAA+TGG 0.373351 2:+8756229 MsaT006713.1:CDS
ATCTCATAATCAAAGTATTT+AGG 0.389618 2:+8756300 MsaT006713.1:CDS
TCAACCTTTGAAACCAATCT+TGG 0.392383 2:-8756023 None:intergenic
GAATCTCTAAAATCCTGCTT+TGG 0.398970 2:-8756179 None:intergenic
CTGCGATTCACTAAGGTACA+TGG 0.426392 2:-8751922 None:intergenic
ACGATAGGCGGCGGCGTTTG+CGG 0.428514 2:-8751967 None:intergenic
AGAAGAAATGGAAGAGTTGT+TGG 0.458484 2:+8756241 MsaT006713.1:CDS
GGAAAAGCATGAAGAGTTGA+AGG 0.459523 2:+8755938 MsaT006713.1:CDS
AAAGATGAAGAAAGAAATTG+TGG 0.494347 2:+8756094 MsaT006713.1:CDS
TGAAGAGAAACAGATTAGTA+AGG 0.497006 2:+8756217 MsaT006713.1:CDS
GGAAACCAAAGAAAGTTTCA+AGG 0.508120 2:+8755985 MsaT006713.1:CDS
TTCACCAAGATTGGTTTCAA+AGG 0.510346 2:+8756019 MsaT006713.1:CDS
CATAATCAAAGTATTTAGGC+AGG 0.542446 2:+8756304 MsaT006713.1:CDS
TCTCTTTCTAGATTGAAAAG+TGG 0.543229 2:+8756795 MsaT006713.1:intron
ATGTTCATCAAATCCAAAGC+AGG 0.550210 2:+8756166 MsaT006713.1:CDS
TAAAAGCATTAGAAGAAATG+AGG 0.552124 2:+8756057 MsaT006713.1:CDS
GTTAATCTGGTTTGGGACTG+AGG 0.553096 2:+8756863 MsaT006713.1:CDS
GAAACCAAAGAAAGTTTCAA+GGG 0.604643 2:+8755986 MsaT006713.1:CDS
GATGAAGAAAGAAATTGTGG+AGG 0.614642 2:+8756097 MsaT006713.1:CDS
TCGAGACTTACGATAGGCGG+CGG 0.664477 2:-8751976 None:intergenic
TATTCGAGACTTACGATAGG+CGG 0.716091 2:-8751979 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 8751924 8756883 8751924 ID=MsaG006713
Chr2 mRNA 8751924 8756883 8751924 ID=MsaT006713.1;Parent=MsaG006713
Chr2 exon 8751924 8751987 8751924 ID=MsaT006713.1.exon1;Parent=MsaT006713.1
Chr2 CDS 8751924 8751987 8751924 ID=cds.MsaT006713.1;Parent=MsaT006713.1
Chr2 exon 8755838 8756325 8755838 ID=MsaT006713.1.exon2;Parent=MsaT006713.1
Chr2 CDS 8755838 8756325 8755838 ID=cds.MsaT006713.1;Parent=MsaT006713.1
Chr2 exon 8756806 8756883 8756806 ID=MsaT006713.1.exon3;Parent=MsaT006713.1
Chr2 CDS 8756806 8756883 8756806 ID=cds.MsaT006713.1;Parent=MsaT006713.1
Gene Sequence

>MsaT006713.1

ATGTACCTTAGTGAATCGCAGAAAAATCTTCGTCAGTCAATCGCCGCAAACGCCGCCGCCTATCATTCGACGAAGAATTCTTCTAGCTTAAGAAATATTCGTTTTGAGAAAAGTGAGATTGCATCATCTCAGAAGAAACAAAGTAATTATGAGAAGCATAGTTTGGAAAAGCATGAAGAGTTGAAGGTAAAGACTCAAGAAAAACAAAGGAGGAAACCAAAGAAAGTTTCAAGGGTGAAAATATATTCACCAAGATTGGTTTCAAAGGTTGAAATATGCAGAATAAAAGCATTAGAAGAAATGAGGAACAAAGCAAAACAAAAGATGAAGAAAGAAATTGTGGAGGAAATTATTAGTACTAAACCAGAATTAGATAGTTTTGCTGTGATTAAATGTTCATCAAATCCAAAGCAGGATTTTAGAGATTCAATGATTGAGATGATTGAAGAGAAACAGATTAGTAAGGCAGAAGAAATGGAAGAGTTGTTGGCATGTTATTTGACTTTGAATGCTGATGAGTATCATGATCTCATAATCAAAGTATTTAGGCAGATTGAAAAGTGGATACTTATCGACATCTATAAAAAAGGAAGAAAAAATTATTTACTAGTTAATCTGGTTTGGGACTGA

Protein sequence

>MsaT006713.1

MYLSESQKNLRQSIAANAAAYHSTKNSSSLRNIRFEKSEIASSQKKQSNYEKHSLEKHEELKVKTQEKQRRKPKKVSRVKIYSPRLVSKVEICRIKALEEMRNKAKQKMKKEIVEEIISTKPELDSFAVIKCSSNPKQDFRDSMIEMIEEKQISKAEEMEELLACYLTLNADEYHDLIIKVFRQIEKWILIDIYKKGRKNYLLVNLVWD*