AlfalfaGEDB | MsaG006714

M. sativa ssp. caerulea / MsaG006714

KEGG: K09419
GO: -
PFAM: PF00447.20

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG006714	XP_003593809.2	76.823	384	49	2	1	384	1	344	0.0	563

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG006714	sp\|O49403\|HFA4A_ARATH	44.308	325	166	7	1	316	1	319	2.13e-72	234

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG006714	tr\|G7ILT3\|G7ILT3_MEDTR	76.823	384	49	2	1	384	1	344	0.0	563

TFs/TRs:

Gene ID	Type	Classification
MsaG006714	TF	HSF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG000722	MsaG006714	0.816976	7.280245e-52	1.480024e-49
MsaG001273	MsaG006714	0.817819	4.712033e-52	9.789510e-50
MsaG006221	MsaG006714	0.800487	2.355857e-48	3.220438e-46
MsaG006714	MsaG006801	0.910943	2.583733e-82	2.596800e-78
MsaG006714	MsaG007182	0.814540	2.527134e-51	4.828766e-49
MsaG006714	MsaG008995	0.845399	7.810944e-59	3.626559e-56
MsaG006714	MsaG009345	0.801645	1.368972e-48	1.921065e-46
MsaG006714	MsaG010221	0.800275	2.600931e-48	3.538400e-46
MsaG006714	MsaG011177	0.806295	1.493871e-49	2.334652e-47
MsaG006714	MsaG011383	0.802269	1.020711e-48	1.452832e-46
MsaG006714	MsaG011472	0.801741	1.308669e-48	1.840456e-46
MsaG006714	MsaG012287	0.830410	5.367200e-55	1.571646e-52
MsaG006714	MsaG015809	0.804561	3.436315e-49	5.156963e-47
MsaG006714	MsaG019525	0.809196	3.637076e-50	6.091575e-48
MsaG006714	MsaG019795	0.810732	1.705038e-50	2.964120e-48
MsaG006714	MsaG020336	0.813438	4.411720e-51	8.200103e-49
MsaG006714	MsaG020815	0.817497	5.564979e-52	1.146604e-49
MsaG006714	MsaG032702	0.808370	5.452700e-50	8.952736e-48
MsaG006714	MsaG033995	0.857003	4.267620e-62	2.965198e-59
MsaG006714	MsaG041202	0.800407	2.444834e-48	3.336160e-46
MsaG006714	MsaG041237	0.807076	1.023912e-49	1.629856e-47
MsaG006714	MsaG043350	0.807188	9.695574e-50	1.547450e-47
MsaG006714	MsaG044019	0.806147	1.604627e-49	2.498833e-47
MsaG006714	MsaG046471	0.808443	5.262120e-50	8.655061e-48
MsaG006714	MsaG007986	0.818413	3.463414e-52	7.306395e-50
MsaG006714	MsaG031242	0.808661	4.728711e-50	7.818946e-48
MsaG006714	MsaG035299	0.814190	3.017384e-51	5.715202e-49
MsaG006714	MsaG037897	0.811341	1.259892e-50	2.223197e-48
MsaG006714	MsaG046905	0.800980	1.870713e-48	2.585827e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG006714	MtrunA17_Chr2g0284481	76.823	384	49	2	1	384	1	344	0.0	563
MsaG006714	MtrunA17_Chr8g0379701	48.615	325	161	3	2	325	1	320	6.16e-103	310
MsaG006714	MtrunA17_Chr5g0410881	45.405	185	97	2	14	194	31	215	1.51e-52	182
MsaG006714	MtrunA17_Chr4g0039891	40.741	189	101	2	13	194	10	194	2.37e-47	168
MsaG006714	MtrunA17_Chr3g0135831	39.320	206	112	3	14	212	45	244	3.57e-45	159
MsaG006714	MtrunA17_Chr7g0260331	40.884	181	107	0	14	194	48	228	3.29e-44	157
MsaG006714	MtrunA17_Chr4g0071051	42.079	202	104	4	14	215	100	288	1.04e-43	157
MsaG006714	MtrunA17_Chr6g0467041	61.111	108	42	0	5	112	12	119	2.79e-43	153
MsaG006714	MtrunA17_Chr2g0330821	40.201	199	106	3	2	194	60	251	2.76e-42	151
MsaG006714	MtrunA17_Chr1g0204741	38.830	188	111	1	6	193	31	214	1.02e-41	149
MsaG006714	MtrunA17_Chr8g0378061	43.017	179	88	4	16	191	23	190	1.70e-41	152
MsaG006714	MtrunA17_Chr8g0392001	43.548	186	101	2	13	194	20	205	3.42e-41	149
MsaG006714	MtrunA17_Chr2g0301521	53.957	139	64	0	99	237	1	139	1.86e-40	141
MsaG006714	MtrunA17_Chr6g0485571	40.884	181	100	2	13	193	9	182	3.26e-40	145
MsaG006714	MtrunA17_Chr5g0402291	42.197	173	70	2	15	157	9	181	3.68e-40	144
MsaG006714	MtrunA17_Chr1g0185131	37.234	188	104	2	6	193	23	196	1.22e-38	141
MsaG006714	MtrunA17_Chr8g0375041	42.500	160	76	3	13	157	7	165	2.92e-38	138
MsaG006714	MtrunA17_Chr5g0397881	57.944	107	44	1	3	108	11	117	4.94e-38	140
MsaG006714	MtrunA17_Chr3g0134031	60.000	100	39	1	9	107	17	116	5.94e-36	135
MsaG006714	MtrunA17_Chr1g0203601	55.660	106	46	1	4	108	6	111	1.45e-35	132
MsaG006714	MtrunA17_Chr5g0441531	58.000	100	41	1	9	107	18	117	9.99e-34	126
MsaG006714	MtrunA17_Chr7g0257331	48.571	105	54	0	15	119	22	126	1.21e-32	122
MsaG006714	MtrunA17_Chr6g0472421	57.609	92	39	0	15	106	33	124	2.02e-32	124
MsaG006714	MtrunA17_Chr4g0023771	65.333	75	25	1	1	74	1	75	6.31e-28	106
MsaG006714	MtrunA17_Chr4g0047421	36.810	163	87	3	15	165	33	191	4.90e-27	107
MsaG006714	MtrunA17_Chr1g0179321	59.677	62	25	0	13	74	47	108	1.25e-22	91.7
MsaG006714	MtrunA17_Chr2g0330841	27.586	203	115	7	75	258	41	230	9.77e-13	69.3
MsaG006714	MtrunA17_Chr4g0023781	46.341	82	31	1	290	371	3	71	1.27e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG006714	AT4G18880.1	44.308	325	166	7	1	316	1	319	2.17e-73	234
MsaG006714	AT5G45710.3	38.920	352	167	7	2	353	1	304	1.14e-64	210
MsaG006714	AT5G45710.1	38.920	352	167	7	2	353	1	304	1.14e-64	210
MsaG006714	AT4G17750.1	43.979	191	96	1	14	193	51	241	1.32e-50	177
MsaG006714	AT1G32330.2	42.408	191	104	2	9	193	31	221	1.47e-47	169
MsaG006714	AT1G32330.1	42.408	191	104	2	9	193	31	221	1.54e-47	169
MsaG006714	AT2G26150.1	38.647	207	116	2	14	212	43	246	2.07e-47	165
MsaG006714	AT2G26150.4	38.647	207	116	2	14	212	43	246	2.07e-47	165
MsaG006714	AT3G02990.1	41.530	183	106	1	13	194	21	203	4.49e-46	164
MsaG006714	AT5G16820.1	40.323	186	109	1	11	194	23	208	5.42e-45	162
MsaG006714	AT5G16820.2	40.323	186	109	1	11	194	23	208	5.42e-45	162
MsaG006714	AT4G13980.1	39.216	204	110	3	16	213	24	219	8.30e-45	161
MsaG006714	AT5G03720.2	37.838	185	104	2	13	194	53	229	5.61e-43	155
MsaG006714	AT5G03720.1	37.838	185	104	2	13	194	53	229	5.61e-43	155
MsaG006714	AT3G22830.1	40.217	184	106	1	14	193	60	243	8.28e-43	154
MsaG006714	AT3G24520.1	39.779	181	83	2	13	193	15	169	4.36e-40	145
MsaG006714	AT5G62020.1	51.880	133	56	2	2	133	9	134	6.65e-40	144
MsaG006714	AT1G67970.1	39.698	199	115	4	13	209	17	212	1.02e-39	145
MsaG006714	AT5G43840.1	42.473	186	84	5	12	193	14	180	2.43e-39	142
MsaG006714	AT3G63350.1	38.710	186	93	3	15	193	28	199	6.14e-38	138
MsaG006714	AT3G51910.1	35.638	188	103	2	6	193	20	189	6.30e-38	138
MsaG006714	AT1G46264.1	58.333	108	41	2	1	107	22	126	2.22e-37	138
MsaG006714	AT5G54070.1	36.765	204	108	5	13	214	69	253	3.79e-35	132
MsaG006714	AT4G36990.1	57.576	99	42	0	15	113	14	112	4.19e-35	130
MsaG006714	AT2G41690.1	54.369	103	46	1	14	115	39	141	9.08e-35	129
MsaG006714	AT4G11660.1	59.783	92	37	0	15	106	59	150	3.45e-34	130
MsaG006714	AT5G45710.4	30.268	261	135	6	99	359	1	214	2.38e-23	98.2
MsaG006714	AT5G45710.2	30.268	261	135	6	99	359	1	214	2.38e-23	98.2
MsaG006714	AT4G18870.1	47.727	88	46	0	15	102	146	233	9.61e-22	94.7
MsaG006714	AT4G18870.1	45.545	101	53	2	2	102	1	99	1.14e-21	94.4
MsaG006714	AT1G77570.1	37.778	90	53	2	16	102	27	116	1.62e-15	73.6
MsaG006714	AT2G26150.3	27.397	146	95	2	75	212	49	191	1.24e-14	74.3
MsaG006714	AT2G26150.2	27.397	146	95	2	75	212	49	191	1.24e-14	74.3

Find 74 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CGCATGACGGATTGTTCTTC+TGG	0.207001	2:+8763950	None:intergenic
CAACTGATGACATTGTTTCT+TGG	0.248061	2:-8765614	MsaT006714.1:CDS
AGTTGCATACAAGATGTTAT+TGG	0.278275	2:+8764208	None:intergenic
AACTGATGACATTGTTTCTT+GGG	0.286701	2:-8765613	MsaT006714.1:CDS
TCTCACTAAGACTTATGATA+TGG	0.289830	2:-8765649	MsaT006714.1:CDS
TCTTCTGGTTCAACAAAATC+AGG	0.301153	2:+8763965	None:intergenic
TGAAACTTGAGAAGTTATTA+TGG	0.310950	2:+8765517	None:intergenic
ATGCTAGCTACATTACAAAA+AGG	0.320103	2:+8764433	None:intergenic
GAAAAGTTGATACACAACTA+TGG	0.322439	2:-8764852	MsaT006714.1:CDS
TTATTATAATCAGTGGTAAC+CGG	0.354372	2:+8763925	None:intergenic
AGCTCAGTTGCATCATGATT+AGG	0.360536	2:+8764082	None:intergenic
GGCTCAGTTGCAACTGGATT+AGG	0.367439	2:+8764043	None:intergenic
CGGATGTCTTTGAAAATAAG+AGG	0.384067	2:+8764270	None:intergenic
AAGAAACCTGGGAATGAGTT+AGG	0.397323	2:-8764511	MsaT006714.1:CDS
CATGTCGACTTCTGGTGACC+TGG	0.398347	2:+8764168	None:intergenic
ACAGTACTCGAAGGGTCATT+TGG	0.400137	2:-8764587	MsaT006714.1:CDS
GGCTCAGTTGCAACTGGATC+AGG	0.407148	2:+8764121	None:intergenic
GTTTCTTGAAATTCAGAGAA+AGG	0.442458	2:-8764641	MsaT006714.1:CDS
ACTGCTGGCTCAGTTGCAAC+TGG	0.444286	2:+8764037	None:intergenic
GGGAATGATGGAAGAAGCTC+AGG	0.447000	2:-8765700	None:intergenic
GAGAAAAGGAGGCACCTTAT+CGG	0.448416	2:+8765668	None:intergenic
CGAATCATCAATGACGAGTT+GGG	0.464732	2:-8764317	MsaT006714.1:CDS
ACATCCGGCTCAGTTGCAAC+TGG	0.474094	2:+8764115	None:intergenic
ATGGAGTTCATGTCGACTTC+TGG	0.480577	2:+8764160	None:intergenic
GATGACTCAATGAATTATGT+AGG	0.484303	2:-8764235	MsaT006714.1:CDS
CATGAGCATTCTGCGAAGAA+TGG	0.490641	2:+8764752	None:intergenic
CATATACCTAACTCATTCCC+AGG	0.495219	2:+8764505	None:intergenic
GAGTCATCAAAATCTACATT+CGG	0.497020	2:+8764250	None:intergenic
TCATAAGTCTTAGTGAGAAA+AGG	0.497418	2:+8765654	None:intergenic
TGAAATTCAGAGAAAGGAAG+AGG	0.498215	2:-8764635	MsaT006714.1:CDS
TCGCAGAATGCTCATGGCCA+AGG	0.501991	2:-8764745	MsaT006714.1:CDS
GGTCAAGTGCTGAAGAAACC+TGG	0.503718	2:-8764523	MsaT006714.1:CDS
GAACAATCCGTCATGCGTCC+CGG	0.506448	2:-8763944	MsaT006714.1:CDS
TTATTATGGTTAAAGTATTG+AGG	0.507346	2:+8765531	None:intergenic
CGCAGAATGCTCATGGCCAA+GGG	0.508087	2:-8764744	MsaT006714.1:CDS
GAAACAATGTCATCAGTTGA+AGG	0.519956	2:+8765618	None:intergenic
TCGAATCATCAATGACGAGT+TGG	0.521047	2:-8764318	MsaT006714.1:CDS
TGATCCAGTTGCAACTGAGC+CGG	0.522036	2:-8764119	MsaT006714.1:CDS
CTAGTGGAGCAGCAACCCCT+TGG	0.524076	2:+8764728	None:intergenic
AAAAGTTGATACACAACTAT+GGG	0.529213	2:-8764851	MsaT006714.1:CDS
ATGTCGACTTCTGGTGACCT+GGG	0.544064	2:+8764169	None:intergenic
TCAGGGTAGTAGTTCCGATA+AGG	0.544906	2:-8765682	MsaT006714.1:CDS
TCGGAAGCATCGATTACTGC+TGG	0.547244	2:+8764022	None:intergenic
TTAGGATTAACAACTACATC+CGG	0.548377	2:+8764100	None:intergenic
CATTCTTCGCAGAATGCTCA+TGG	0.549403	2:-8764751	MsaT006714.1:CDS
TGTCGATCGGATTCTGCTAG+TGG	0.551922	2:+8764712	None:intergenic
CAAAGTGTGCTCTCTTATGT+TGG	0.554182	2:-8764544	MsaT006714.1:CDS
TAAGACTTATGATATGGTTG+AGG	0.565627	2:-8765643	MsaT006714.1:CDS
TGGCTCAACTGCAACAAGAT+CGG	0.566890	2:+8764003	None:intergenic
AGCATTCAATGTCTGTCGAT+CGG	0.568118	2:+8764699	None:intergenic
AGCTAGCATTGAAGATCATG+TGG	0.568546	2:-8764419	MsaT006714.1:CDS
TATTATAATCAGTGGTAACC+GGG	0.569793	2:+8763926	None:intergenic
GGATCAGGAACAACTACCAC+TGG	0.574913	2:+8764136	None:intergenic
GATAAAATTACAGTACTCGA+AGG	0.578742	2:-8764596	MsaT006714.1:CDS
ACTGATGACATTGTTTCTTG+GGG	0.579964	2:-8765612	MsaT006714.1:CDS
GTCAAGTGCTGAAGAAACCT+GGG	0.583533	2:-8764522	MsaT006714.1:CDS
TCAGGGACAACTGCAACAGT+TGG	0.589380	2:+8763983	None:intergenic
TAATGATAGAAGAGACACAC+TGG	0.597081	2:+8764363	None:intergenic
AGGAACAACTACCACTGGCA+TGG	0.597086	2:+8764141	None:intergenic
CTTCTGGTTCAACAAAATCA+GGG	0.603287	2:+8763966	None:intergenic
GTGGTAACCGGGACGCATGA+CGG	0.609132	2:+8763937	None:intergenic
GTCATTTGGAAACAGTGGAA+AGG	0.611941	2:-8764573	MsaT006714.1:CDS
TAAGTCTTAGTGAGAAAAGG+AGG	0.624943	2:+8765657	None:intergenic
TAGATGTTGAAGTTCAGCCC+AGG	0.629376	2:-8764186	MsaT006714.1:CDS
ATCTTATGAAGAATATCCAA+AGG	0.635890	2:-8764792	MsaT006714.1:CDS
ATAAAATTACAGTACTCGAA+GGG	0.640506	2:-8764595	MsaT006714.1:CDS
GTCTCTTCTATCATTAAACG+TGG	0.666811	2:-8764356	MsaT006714.1:CDS
CGACATGAACTCCATGCCAG+TGG	0.675176	2:-8764152	MsaT006714.1:CDS
GCTAGCATTGAAGATCATGT+GGG	0.675912	2:-8764418	MsaT006714.1:CDS
AATGATAGAAGAGACACACT+GGG	0.677326	2:+8764364	None:intergenic
GAAGGGTCATTTGGAAACAG+TGG	0.685082	2:-8764578	MsaT006714.1:CDS
GCAGAATGCTCATGGCCAAG+GGG	0.714345	2:-8764743	MsaT006714.1:CDS
GGAATGATGGAAGAAGCTCA+GGG	0.733848	2:-8765699	MsaT006714.1:CDS
ATGATAGAAGAGACACACTG+GGG	0.819436	2:+8764365	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	8763934	8765718	8763934	ID=MsaG006714
Chr2	mRNA	8763934	8765718	8763934	ID=MsaT006714.1;Parent=MsaG006714
Chr2	exon	8763934	8764881	8763934	ID=MsaT006714.1.exon2;Parent=MsaT006714.1
Chr2	CDS	8763934	8764881	8763934	ID=cds.MsaT006714.1;Parent=MsaT006714.1
Chr2	exon	8765497	8765718	8765497	ID=MsaT006714.1.exon1;Parent=MsaT006714.1
Chr2	CDS	8765497	8765718	8765497	ID=cds.MsaT006714.1;Parent=MsaT006714.1

Gene Sequence

>MsaT006714.1

ATGATGGAAGAAGCTCAGGGTAGTAGTTCCGATAAGGTGCCTCCTTTTCTCACTAAGACTTATGATATGGTTGAGGATCCTTCAACTGATGACATTGTTTCTTGGGGTGCTACTGATGAAAGTTTTATTGTTTGGAATAAACTAGATTTTGAAAAAGATGTGTTGCCTCAATACTTTAACCATAATAACTTCTCAAGTTTCATCAGACAGCTTAATACCTATGGCTTTAGAAAAGTTGATACACAACTATGGGAATACGCAAACGATGATTTCGTAAGAAATCAAAAACATCTTATGAAGAATATCCAAAGGCGCAAAACAGTTTATAGCCATTCTTCGCAGAATGCTCATGGCCAAGGGGTTGCTGCTCCACTAGCAGAATCCGATCGACAGACATTGAATGCTCATATTCAAAAACTTAAACACGACAATGAACAAATGTTTCTTGAAATTCAGAGAAAGGAAGAGGAGAGAAAAATGCATGAGATAAAATTACAGTACTCGAAGGGTCATTTGGAAACAGTGGAAAGGAAACATCAAAGTGTGCTCTCTTATGTTGGTCAAGTGCTGAAGAAACCTGGGAATGAGTTAGGTATATGTCCGCTGACAAAAACTACAAAGCGAAAAAGGAGGATTCCGAGAAACAATCCTTTTTGTAATGTAGCTAGCATTGAAGATCATGTGGGAACTTCTCATGTAATACATAAAGAAAACGACCCCAGTGTGTCTCTTCTATCATTAAACGTGGAACAATTGAATTTGCTCGAATCATCAATGACGAGTTGGGAGAAGATTAAAAATGATCTTGGTGCCTCTTATTTTCAAAGACATCCGAATGTAGATTTTGATGACTCAATGAATTATGTAGGCTGTCCAATAACATCTTGTATGCAACTAGATGTTGAAGTTCAGCCCAGGTCACCAGAAGTCGACATGAACTCCATGCCAGTGGTAGTTGTTCCTGATCCAGTTGCAACTGAGCCGGATGTAGTTGTTAATCCTAATCATGATGCAACTGAGCTGAATGTAATCGTTGTTCCTAATCCAGTTGCAACTGAGCCAGCAGTAATCGATGCTTCCGATCTTGTTGCAGTTGAGCCAACTGTTGCAGTTGTCCCTGATTTTGTTGAACCAGAAGAACAATCCGTCATGCGTCCCGGTTACCACTGA

Protein sequence

>MsaT006714.1

MMEEAQGSSSDKVPPFLTKTYDMVEDPSTDDIVSWGATDESFIVWNKLDFEKDVLPQYFNHNNFSSFIRQLNTYGFRKVDTQLWEYANDDFVRNQKHLMKNIQRRKTVYSHSSQNAHGQGVAAPLAESDRQTLNAHIQKLKHDNEQMFLEIQRKEEERKMHEIKLQYSKGHLETVERKHQSVLSYVGQVLKKPGNELGICPLTKTTKRKRRIPRNNPFCNVASIEDHVGTSHVIHKENDPSVSLLSLNVEQLNLLESSMTSWEKIKNDLGASYFQRHPNVDFDDSMNYVGCPITSCMQLDVEVQPRSPEVDMNSMPVVVVPDPVATEPDVVVNPNHDATELNVIVVPNPVATEPAVIDASDLVAVEPTVAVVPDFVEPEEQSVMRPGYH*