AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006787

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006787 MtrunA17_Chr2g0285461 88.677 786 32 6 1 729 1 786 0.0 1275
MsaG006787 MtrunA17_Chr5g0433301 56.069 758 272 18 1 729 1 726 0.0 705
MsaG006787 MtrunA17_Chr3g0109391 55.512 762 247 22 13 729 20 734 0.0 704
MsaG006787 MtrunA17_Chr4g0070181 56.073 494 182 14 1 465 1 488 6.13e-157 485
MsaG006787 MtrunA17_Chr1g0156321 63.836 365 115 6 13 363 37 398 1.86e-149 458
MsaG006787 MtrunA17_Chr2g0301251 55.579 484 172 14 13 464 17 489 5.16e-149 464
MsaG006787 MtrunA17_Chr8g0389081 51.724 551 224 12 16 531 29 572 3.23e-148 461
MsaG006787 MtrunA17_Chr2g0276781 50.959 365 165 4 14 366 18 380 1.02e-113 359
MsaG006787 MtrunA17_Chr8g0385791 50.272 368 166 7 13 366 53 417 4.27e-110 354
MsaG006787 MtrunA17_Chr8g0346711 48.980 343 159 5 16 346 26 364 1.79e-103 332
MsaG006787 MtrunA17_Chr4g0029671 41.201 483 222 14 19 456 54 519 3.31e-99 324
MsaG006787 MtrunA17_Chr2g0326281 46.684 377 161 8 19 358 56 429 1.30e-98 323
MsaG006787 MtrunA17_Chr7g0239061 45.553 371 181 9 13 367 8 373 9.50e-97 315
MsaG006787 MtrunA17_Chr4g0009751 47.945 365 161 10 17 361 17 372 3.32e-96 311
MsaG006787 MtrunA17_Chr4g0046371 45.646 379 160 9 19 366 38 401 5.15e-95 310
MsaG006787 MtrunA17_Chr2g0282961 46.218 357 153 7 11 341 45 388 1.18e-94 307
MsaG006787 MtrunA17_Chr2g0326761 36.609 407 197 9 13 365 5 404 8.11e-71 245
MsaG006787 MtrunA17_Chr4g0028971 36.199 442 198 14 16 386 9 437 1.99e-69 241
MsaG006787 MtrunA17_Chr1g0199681 36.207 406 206 9 13 370 88 488 7.76e-68 237
MsaG006787 MtrunA17_Chr1g0181211 34.146 410 182 9 13 352 21 412 2.42e-63 224
MsaG006787 MtrunA17_Chr8g0376771 63.054 203 55 7 537 729 600 792 3.60e-63 228
MsaG006787 MtrunA17_Chr7g0264171 33.040 454 232 14 16 401 28 477 5.20e-58 208
MsaG006787 MtrunA17_Chr3g0115271 34.596 396 206 7 9 358 9 397 1.22e-57 206
MsaG006787 MtrunA17_Chr8g0356211 39.382 259 141 6 119 367 2 254 6.17e-51 180
MsaG006787 MtrunA17_Chr5g0424931 32.817 387 220 11 6 359 6 385 7.82e-51 186
MsaG006787 MtrunA17_Chr7g0264161 34.653 303 186 6 28 320 1 301 1.00e-47 171
MsaG006787 MtrunA17_Chr5g0417281 30.809 383 225 11 13 375 5 367 1.04e-46 174
MsaG006787 MtrunA17_Chr5g0436761 32.044 362 211 11 12 353 3 349 8.78e-43 161
MsaG006787 MtrunA17_Chr1g0178011 30.352 369 226 11 8 359 1 355 1.07e-41 158
MsaG006787 MtrunA17_Chr2g0289221 32.698 315 182 12 6 302 7 309 6.61e-38 145
MsaG006787 MtrunA17_Chr2g0277461 29.745 353 213 12 19 353 13 348 4.76e-35 139
MsaG006787 MtrunA17_Chr5g0417211 30.033 303 187 9 16 302 9 302 1.74e-32 130
MsaG006787 MtrunA17_Chr5g0424741 26.425 386 226 11 10 351 4 375 5.33e-31 128
MsaG006787 MtrunA17_Chr5g0424801 27.778 342 223 10 11 340 6 335 2.42e-28 117
MsaG006787 MtrunA17_Chr5g0425261 27.778 342 223 10 11 340 6 335 3.16e-27 114
MsaG006787 MtrunA17_Chr2g0277531 28.758 306 177 13 19 302 20 306 1.01e-25 108
MsaG006787 MtrunA17_Chr5g0425661 30.631 222 129 6 2 206 20 233 2.48e-25 105
MsaG006787 MtrunA17_Chr1g0156311 46.018 113 28 3 228 340 1 80 1.39e-14 69.7
MsaG006787 MtrunA17_Chr5g0424791 35.156 128 70 5 82 206 34 151 7.04e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006787 AT5G37020.2 52.185 778 261 21 1 729 1 716 0.0 643
MsaG006787 AT5G37020.1 52.185 778 261 21 1 729 1 716 0.0 642
MsaG006787 AT5G37020.3 50.073 683 244 19 82 729 1 621 3.07e-174 518
MsaG006787 AT1G19220.1 54.473 503 177 15 1 466 1 488 2.09e-151 470
MsaG006787 AT5G20730.2 55.876 485 154 14 4 450 5 467 1.38e-147 462
MsaG006787 AT5G20730.3 55.761 486 154 15 4 450 5 468 6.74e-146 457
MsaG006787 AT5G20730.1 55.761 486 154 15 4 450 5 468 1.05e-145 457
MsaG006787 AT1G19850.2 50.509 491 213 10 15 490 39 514 5.89e-143 442
MsaG006787 AT1G19850.1 50.509 491 213 10 15 490 50 525 6.12e-143 442
MsaG006787 AT5G62000.2 50.269 372 168 7 2 359 44 412 2.44e-112 361
MsaG006787 AT5G62000.5 50.269 372 168 7 2 359 44 412 2.44e-112 361
MsaG006787 AT5G62000.3 50.269 372 168 7 2 359 44 412 2.44e-112 361
MsaG006787 AT5G62000.1 50.269 372 168 7 2 359 44 412 2.44e-112 361
MsaG006787 AT5G62000.4 50.269 372 168 7 2 359 44 412 2.86e-112 361
MsaG006787 AT1G59750.4 50.272 368 165 6 14 366 17 381 3.68e-112 355
MsaG006787 AT1G59750.2 50.272 368 165 6 14 366 17 381 4.96e-112 355
MsaG006787 AT1G59750.1 50.272 368 165 6 14 366 17 381 6.17e-112 354
MsaG006787 AT1G59750.3 50.272 368 165 6 14 366 17 381 6.17e-112 354
MsaG006787 AT5G60450.2 49.604 379 155 11 18 365 40 413 3.04e-106 342
MsaG006787 AT5G60450.1 49.604 379 155 11 18 365 64 437 1.08e-105 342
MsaG006787 AT2G46530.1 48.997 349 160 7 16 351 18 361 1.03e-104 333
MsaG006787 AT3G61830.1 49.570 349 157 8 13 347 19 362 2.32e-104 333
MsaG006787 AT2G46530.3 48.997 349 160 7 16 351 39 382 2.93e-104 333
MsaG006787 AT2G33860.1 43.590 390 173 8 19 375 54 429 2.06e-92 301
MsaG006787 AT4G23980.2 46.133 362 172 8 14 359 7 361 2.24e-91 300
MsaG006787 AT4G23980.1 46.133 362 172 8 14 359 7 361 2.43e-91 299
MsaG006787 AT3G61830.2 48.571 315 143 8 47 347 2 311 2.47e-88 289
MsaG006787 AT2G46530.2 51.383 253 118 4 100 351 26 274 1.14e-78 262
MsaG006787 AT1G34310.1 41.761 352 185 7 19 358 27 370 8.16e-78 262
MsaG006787 AT2G33860.2 49.265 272 130 4 111 375 2 272 3.06e-77 256
MsaG006787 AT1G35240.1 40.000 365 199 7 6 358 14 370 5.37e-77 260
MsaG006787 AT1G35540.1 40.286 350 189 7 6 343 14 355 5.54e-77 260
MsaG006787 AT1G34410.1 40.000 365 199 7 6 358 14 370 1.15e-76 259
MsaG006787 AT1G35520.1 40.688 349 187 7 6 342 14 354 3.51e-75 255
MsaG006787 AT1G34170.1 41.194 335 181 9 19 342 25 354 1.84e-71 243
MsaG006787 AT1G34390.1 41.193 352 185 8 19 358 27 368 1.88e-71 245
MsaG006787 AT2G28350.2 36.854 426 187 14 13 371 4 414 1.30e-69 242
MsaG006787 AT2G28350.1 36.854 426 187 14 13 371 4 414 1.69e-69 242
MsaG006787 AT4G30080.1 36.962 395 176 13 13 345 13 396 7.09e-65 229
MsaG006787 AT1G34170.2 37.910 335 166 8 19 342 25 328 1.41e-61 215
MsaG006787 AT1G34170.3 37.910 335 166 8 19 342 25 328 7.77e-61 214
MsaG006787 AT1G77850.1 33.079 393 212 9 16 363 16 402 9.38e-51 187
MsaG006787 AT1G77850.2 33.079 393 212 9 16 363 16 402 1.04e-50 187
MsaG006787 AT1G43950.1 43.069 202 98 4 19 209 27 222 3.99e-44 159
MsaG006787 AT1G30330.1 47.115 208 82 13 540 729 608 805 1.85e-30 129
MsaG006787 AT1G30330.3 47.115 208 82 13 540 729 610 807 1.95e-30 129
MsaG006787 AT1G30330.2 47.115 208 82 13 540 729 610 807 1.95e-30 129

Find 227 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAGTTGGTATGCGTTTC+AGG 0.162161 2:-9854219 MsaT006787.1:CDS
TAATGGGATGTCGAACTTTA+AGG 0.164117 2:-9852488 MsaT006787.1:CDS
GATAATAATCAGCAGATTTC+TGG 0.177527 2:-9852982 MsaT006787.1:intron
TGCTCCCTTCTTGGTCTATT+GGG 0.181638 2:+9852572 None:intergenic
GCGGATCATTGCTCCCTTCT+TGG 0.200523 2:+9852563 None:intergenic
AGATCCTTCCAAACAGCTTC+TGG 0.207005 2:-9853256 MsaT006787.1:CDS
TTGCTCCCTTCTTGGTCTAT+TGG 0.213710 2:+9852571 None:intergenic
GTGATTCTGTGCTGTTTATC+TGG 0.230987 2:-9854837 MsaT006787.1:intron
GAAAAGAACCAATTGCTTCT+TGG 0.261328 2:-9854532 MsaT006787.1:CDS
GGAGGTAAGCTTGGATAGTT+TGG 0.269119 2:+9855664 None:intergenic
CTCTTAATTATCAAGGGATT+GGG 0.281845 2:-9853386 MsaT006787.1:CDS
AAGGGATTGGGGTTAATTCT+TGG 0.291066 2:-9853374 MsaT006787.1:CDS
AGTTGTAAGTAGATGCCTTT+TGG 0.291101 2:+9854900 None:intergenic
TTGTTGCAGGTTTATAAATC+AGG 0.292598 2:-9852189 MsaT006787.1:intron
GGAACCCAAGAGCTTGTTCT+TGG 0.317973 2:+9852761 None:intergenic
TCATCTCTTCTAATGCATAA+TGG 0.318760 2:-9852505 MsaT006787.1:CDS
TGTGGCTGCTACTGCACTTC+AGG 0.319159 2:-9853295 MsaT006787.1:CDS
TACATAGGAAATGTTGTTAA+TGG 0.322032 2:+9853692 None:intergenic
AGGTCACAAATGCCAGTTAT+TGG 0.326550 2:+9853994 None:intergenic
CATCTCTTCTAATGCATAAT+GGG 0.330297 2:-9852504 MsaT006787.1:CDS
GAACAAACACATACCTTCTT+GGG 0.341095 2:+9856779 None:intergenic
TTCTCCTTTGCACAGTATCA+TGG 0.346541 2:-9852828 MsaT006787.1:CDS
GGCATGAAAGATGATGATTT+TGG 0.347577 2:-9853459 MsaT006787.1:CDS
CTGCCTCAGGTGGAGCAGTT+AGG 0.348006 2:-9852658 MsaT006787.1:CDS
CTTGGAGTTCCCAGCTCTGC+TGG 0.355032 2:+9855331 None:intergenic
TTAAAGCAGCGGTCCTGTTC+GGG 0.371765 2:+9853199 None:intergenic
TGAACAAACACATACCTTCT+TGG 0.374610 2:+9856778 None:intergenic
GGTGGATCCACTCCTCTCTT+CGG 0.375518 2:-9852698 MsaT006787.1:CDS
AGATACATGGGTCCAATAAC+TGG 0.375695 2:-9854006 MsaT006787.1:CDS
TCTCTTAATTATCAAGGGAT+TGG 0.381630 2:-9853387 MsaT006787.1:CDS
CAAGGAACCCAGAAGCTGTT+TGG 0.386898 2:+9853248 None:intergenic
CTCTTGAGCAGGAGGCTGTT+GGG 0.387165 2:+9855112 None:intergenic
CCTGGATTGCCTTCGTTCCA+TGG 0.388608 2:-9853629 MsaT006787.1:intron
GTTGAGTTTGTTGTTGCTGC+TGG 0.388634 2:+9853016 None:intergenic
GGCCTTTCAAAATAATAATC+AGG 0.388931 2:-9853139 MsaT006787.1:CDS
GTGGATCCACTCCTCTCTTC+GGG 0.390379 2:-9852697 MsaT006787.1:CDS
CTCTTGCAGGCCAGCCCAAA+AGG 0.390511 2:-9854915 MsaT006787.1:intron
CCATGGAACGAAGGCAATCC+AGG 0.392004 2:+9853629 None:intergenic
CTTCAATCTCTTAATTATCA+AGG 0.393969 2:-9853393 MsaT006787.1:CDS
TCATGTAATTAGAAGATTGA+TGG 0.401440 2:+9852425 None:intergenic
GACTTGGATCGCAGGTTGGT+TGG 0.404773 2:-9853769 MsaT006787.1:intron
GAGGTAAGCTTGGATAGTTT+GGG 0.407695 2:+9855665 None:intergenic
CACTCTAGGTTGCCTTTCTC+CGG 0.408251 2:+9853733 None:intergenic
CGCCTTCCAGTCCGAACATT+CGG 0.413325 2:+9852101 None:intergenic
GTCTTCCAAGAACAAGCTCT+TGG 0.413478 2:-9852766 MsaT006787.1:CDS
GCAACAGTCGCAGCCTCAAC+AGG 0.414241 2:-9853160 MsaT006787.1:CDS
CTTGTCTCTTTGCCAGCAGT+TGG 0.417884 2:-9856176 MsaT006787.1:CDS
ATTAAAGCAGCGGTCCTGTT+CGG 0.418907 2:+9853198 None:intergenic
GGGATTCTCCGTGCCTCGCC+GGG 0.419026 2:-9855248 MsaT006787.1:CDS
CCAATGAGACTCTCTTCAGC+TGG 0.421483 2:-9856816 MsaT006787.1:CDS
TTCTTGCATACCCAGGAAAA+CGG 0.422042 2:-9852340 MsaT006787.1:CDS
GAGGCACCTTCCAGCAGAGC+TGG 0.422946 2:-9855341 MsaT006787.1:CDS
TAGTTTGTCGGCTGTTTACT+TGG 0.426749 2:+9855313 None:intergenic
CTTAAACGACCGTGGCCTCC+TGG 0.428239 2:-9853647 MsaT006787.1:CDS
ATCCGTCACCATTCCCTCTC+AGG 0.433049 2:-9853670 MsaT006787.1:CDS
TGCCTCAGGTGGAGCAGTTA+GGG 0.438031 2:-9852657 MsaT006787.1:CDS
AGTGAGCTTCGCCGAATGTT+CGG 0.438476 2:-9852112 MsaT006787.1:CDS
ATTACATTACCACCGTTTCC+TGG 0.440592 2:-9852604 MsaT006787.1:CDS
TCTTGCATACCCAGGAAAAC+GGG 0.442241 2:-9852339 MsaT006787.1:CDS
CCAGCTGAAGAGAGTCTCAT+TGG 0.442653 2:+9856816 None:intergenic
CGCCAAGGGTATGACAAATT+CGG 0.445368 2:+9854273 None:intergenic
AAAAGGCATCTACTTACAAC+TGG 0.447091 2:-9854898 MsaT006787.1:CDS
GGTTTATAAATCAGGGTCCT+TGG 0.447324 2:-9852181 MsaT006787.1:CDS
GAAAATAGTGAAACGGCTAT+TGG 0.448976 2:+9854405 None:intergenic
GATCCTTCCAAACAGCTTCT+GGG 0.451775 2:-9853255 MsaT006787.1:CDS
GCGGTCCTGTTCGGGAAGTT+CGG 0.455221 2:+9853207 None:intergenic
AGCCACAGCTTGATACATAT+CGG 0.455527 2:+9853311 None:intergenic
TCTCCTTTGCACAGTATCAT+GGG 0.455578 2:-9852827 MsaT006787.1:CDS
ACTTGGATCGCAGGTTGGTT+GGG 0.456236 2:-9853768 MsaT006787.1:intron
GGCAACCTAGAGTGTCTTTG+TGG 0.458141 2:-9853724 MsaT006787.1:CDS
GCTGATTCTTCGTTGAATCA+TGG 0.461747 2:-9852391 MsaT006787.1:CDS
CATGGAGTAACGCCTGGTAT+TGG 0.463985 2:-9852373 MsaT006787.1:CDS
GAACTTTGGCATGCATGTGC+TGG 0.467783 2:-9856203 MsaT006787.1:CDS
AAACACCGAACTTCCCGAAC+AGG 0.469518 2:-9853212 MsaT006787.1:CDS
ATCTCAATGTATTCTGCCTC+AGG 0.469855 2:-9852671 MsaT006787.1:CDS
AGGTTGCCTTTCTCCGGCAG+TGG 0.470273 2:+9853739 None:intergenic
GTCTTGCAAAAGTAGTTTGT+CGG 0.474515 2:+9855301 None:intergenic
GGGGATTCTCCGTGCCTCGC+CGG 0.474560 2:-9855249 MsaT006787.1:CDS
ACCCAGGAAAACGGGGATCA+AGG 0.476331 2:-9852331 MsaT006787.1:CDS
TTCAGCCGCCCGGCGAGGCA+CGG 0.476974 2:+9855240 None:intergenic
TCTTCCAAGAACAAGCTCTT+GGG 0.477287 2:-9852765 MsaT006787.1:CDS
ATAAGCTGAGGAGGTAAGCT+TGG 0.477482 2:+9855655 None:intergenic
CCGAATAAAAGATTACTTTG+CGG 0.478680 2:+9852544 None:intergenic
ATCCGAATTTGTCATACCCT+TGG 0.484834 2:-9854275 MsaT006787.1:CDS
GAGTTCTAGATTCAGAACTT+TGG 0.485224 2:-9856217 MsaT006787.1:CDS
AATGGGATGTCGAACTTTAA+GGG 0.487362 2:-9852487 MsaT006787.1:CDS
GAGGAAGGCATCACAGTTTG+TGG 0.487690 2:+9854490 None:intergenic
TTGTTCTTGGAAGACTCATA+AGG 0.488411 2:+9852774 None:intergenic
CGACAAATATTTACCAAAGG+AGG 0.491583 2:+9855208 None:intergenic
ACTCTTGAGCAGGAGGCTGT+TGG 0.493884 2:+9855111 None:intergenic
CTCACATTGGCGCTCGGTAA+AGG 0.494139 2:-9853950 MsaT006787.1:intron
TTCAATCTCTTAATTATCAA+GGG 0.495099 2:-9853392 MsaT006787.1:CDS
GCATGAATTCTCCTTTGTTG+TGG 0.501682 2:-9853437 MsaT006787.1:CDS
GTTTGCAGCAAGAGCAAAAG+AGG 0.502339 2:-9855360 MsaT006787.1:intron
ATGTGCATCGACTTCCTTGT+TGG 0.502862 2:+9855690 None:intergenic
ATGAATCCACTGCCGGAGAA+AGG 0.504170 2:-9853745 MsaT006787.1:CDS
TCTATTGGGCACTCCCTACC+AGG 0.505062 2:+9852586 None:intergenic
TGGCTCCGAGACACGGATAG+AGG 0.506451 2:-9853417 MsaT006787.1:CDS
CGTTTAAGCCTGAGAGGGAA+TGG 0.506571 2:+9853662 None:intergenic
ATTACCACCGTTTCCTGGTA+GGG 0.508698 2:-9852599 MsaT006787.1:CDS
CAGTTTGTGGTCTGCTAGCA+CGG 0.509265 2:+9854503 None:intergenic
CACATACCTTCTTGGGGTGG+AGG 0.510763 2:+9856786 None:intergenic
CTAGTGACACAAGCACTCAC+GGG 0.513763 2:-9855270 MsaT006787.1:CDS
CAGGACCGCTGCTTTAATGC+AGG 0.514239 2:-9853193 MsaT006787.1:CDS
TGCCCTAACTGCTCCACCTG+AGG 0.514830 2:+9852655 None:intergenic
AGTGCTAAAAGACTTGTTGC+TGG 0.517965 2:-9854859 MsaT006787.1:CDS
CTTGCTATTAACTCTTGAGC+AGG 0.523192 2:+9855101 None:intergenic
TTCACAAAGGTCTTGTTCAG+TGG 0.523923 2:+9852301 None:intergenic
TCTGCCACCGGACAGAATCC+AGG 0.524242 2:+9853974 None:intergenic
GGCGGATACCAAGAAGCAAT+TGG 0.526575 2:+9854524 None:intergenic
TGTTGCAGGTTTATAAATCA+GGG 0.529690 2:-9852188 MsaT006787.1:intron
GAGTTCCCAGCTCTGCTGGA+AGG 0.530943 2:+9855335 None:intergenic
GTTCGGACTGGAAGGCGAGT+TGG 0.531084 2:-9852095 MsaT006787.1:CDS
GTCAAGTGATAGTATGCACT+TGG 0.531718 2:-9854461 MsaT006787.1:CDS
AATAACTGGCATTTGTGACC+TGG 0.532832 2:-9853992 MsaT006787.1:CDS
TCACAAAGGTCTTGTTCAGT+GGG 0.532882 2:+9852302 None:intergenic
GCCGTCGAAGCGCATTGCGG+TGG 0.532901 2:-9852716 MsaT006787.1:intron
TCTTGCTGCAGCAGCTCATG+CGG 0.533897 2:-9854434 MsaT006787.1:CDS
TTAATTCTTGGATGCAGCCA+AGG 0.534781 2:-9853362 MsaT006787.1:CDS
TTGAATCATGGAGTAACGCC+TGG 0.538445 2:-9852379 MsaT006787.1:CDS
TCCTGGGTATGCAAGAAACC+AGG 0.538483 2:+9852346 None:intergenic
ACGCCTGGTATTGGTGAACC+TGG 0.541034 2:-9852364 MsaT006787.1:CDS
TCAGGTGGAGCAGTTAGGGC+AGG 0.541919 2:-9852653 MsaT006787.1:CDS
GGCATCTACTTACAACTGGA+TGG 0.547248 2:-9854894 MsaT006787.1:CDS
CAGGATGCTCTTTGAAACAG+AGG 0.547415 2:-9854200 MsaT006787.1:CDS
GGTTATAGAAAATAGTGAAA+CGG 0.547772 2:+9854398 None:intergenic
GTTTGCATATTAAGCATGGC+GGG 0.548627 2:+9853333 None:intergenic
CGCCGAATGTTCGGACTGGA+AGG 0.550148 2:-9852103 MsaT006787.1:CDS
CAGGGTCCTTGGAAGATCGT+TGG 0.550235 2:-9852170 MsaT006787.1:CDS
GAAGGCAATCCAGGAGGCCA+CGG 0.550715 2:+9853638 None:intergenic
TCTTAATTATCAAGGGATTG+GGG 0.550737 2:-9853385 MsaT006787.1:CDS
GCTAGTGACACAAGCACTCA+CGG 0.551619 2:-9855271 MsaT006787.1:CDS
ATTTCCCACAAAGACACTCT+AGG 0.551924 2:+9853719 None:intergenic
GGGCACTCCCTACCAGGAAA+CGG 0.553138 2:+9852592 None:intergenic
GGGAATGGTGACGGATACAT+AGG 0.554939 2:+9853677 None:intergenic
GCAACCTAGAGTGTCTTTGT+GGG 0.555847 2:-9853723 MsaT006787.1:CDS
GCTTGTGTCACTAGCAGTCA+AGG 0.559088 2:+9855279 None:intergenic
CATTACCACCGTTTCCTGGT+AGG 0.559493 2:-9852600 MsaT006787.1:CDS
TTTGTGACCTGGATTCTGTC+CGG 0.562740 2:-9853981 MsaT006787.1:CDS
ATCGGTTTGCATATTAAGCA+TGG 0.564195 2:+9853329 None:intergenic
GAGTGCCCAATAGACCAAGA+AGG 0.564439 2:-9852577 MsaT006787.1:CDS
TTTGTTGTGGCTCCGAGACA+CGG 0.566247 2:-9853424 MsaT006787.1:CDS
TTGATGGTAGGACATGGTTG+AGG 0.567713 2:+9852441 None:intergenic
TGCTCAAGAGTTAATAGCAA+GGG 0.572218 2:-9855099 MsaT006787.1:CDS
GAAGCCCTCTATCCGTGTCT+CGG 0.572260 2:+9853412 None:intergenic
ACAACTCTACTTCCAACTGC+TGG 0.574124 2:+9856164 None:intergenic
CGGACTGGAAGGCGAGTTGG+AGG 0.574126 2:-9852092 MsaT006787.1:CDS
TTTAGTCCTCCACCCCAAGA+AGG 0.575086 2:-9856792 MsaT006787.1:intron
TTCTTGGAAGACTCATAAGG+TGG 0.575295 2:+9852777 None:intergenic
ACTTGTTCACTGTGACCTTG+TGG 0.575829 2:+9856134 None:intergenic
TACAGCATTCAGAGGTTGCA+AGG 0.577363 2:+9855460 None:intergenic
AGGCACCTTCCAGCAGAGCT+GGG 0.577521 2:-9855340 MsaT006787.1:CDS
GGGATTTGCATGGTAACGAA+TGG 0.578891 2:-9855079 MsaT006787.1:CDS
GCCAATGTGAGTTCTGCCAC+CGG 0.579314 2:+9853962 None:intergenic
AGTGCCCAATAGACCAAGAA+GGG 0.582142 2:-9852576 MsaT006787.1:CDS
TATCATACTCGAGTTTCAGT+TGG 0.582440 2:-9854232 MsaT006787.1:CDS
AGCTTTAACATACTTCGCCA+AGG 0.582602 2:+9854258 None:intergenic
TGAGGATTAGTCTGAGCTTG+TGG 0.584983 2:+9853090 None:intergenic
GAACCCAGAAGCTGTTTGGA+AGG 0.588893 2:+9853252 None:intergenic
ACCTGGTTTCTTGCATACCC+AGG 0.589141 2:-9852347 MsaT006787.1:CDS
GTTGCTGCTGGTTATGATGA+CGG 0.589209 2:+9853028 None:intergenic
GGTTGGGATGAATCCACTGC+CGG 0.589399 2:-9853752 MsaT006787.1:CDS
GCAGAACTCACATTGGCGCT+CGG 0.590336 2:-9853956 MsaT006787.1:CDS
GCTTCGCCGAATGTTCGGAC+TGG 0.591896 2:-9852107 MsaT006787.1:CDS
ATTATGCATTAGAAGAGATG+AGG 0.592095 2:+9852507 None:intergenic
GCTATTAACTCTTGAGCAGG+AGG 0.592105 2:+9855104 None:intergenic
AGCATGGCGGGATCAAACCT+TGG 0.593115 2:+9853345 None:intergenic
TCTAATTACATGAACACCAC+AGG 0.594162 2:-9852415 MsaT006787.1:CDS
TGATGCTGTAGATGCTGTTG+AGG 0.596375 2:+9853072 None:intergenic
ATGGGATGTCGAACTTTAAG+GGG 0.597378 2:-9852486 MsaT006787.1:CDS
TCAATGTATTCTGCCTCAGG+TGG 0.598371 2:-9852668 MsaT006787.1:CDS
TGATATCCAACGATCTTCCA+AGG 0.601364 2:+9852164 None:intergenic
ATTGAGATGCTCCCGAAGAG+AGG 0.602086 2:+9852686 None:intergenic
AGCCTGAGAGGGAATGGTGA+CGG 0.604684 2:+9853668 None:intergenic
GGCTCCGAGACACGGATAGA+GGG 0.613419 2:-9853416 MsaT006787.1:CDS
TTATGCATTAGAAGAGATGA+GGG 0.615668 2:+9852508 None:intergenic
AGTTGACAAATAAGCTGAGG+AGG 0.618727 2:+9855646 None:intergenic
ACAACAAACTCAACAGCAAG+TGG 0.620384 2:-9853007 MsaT006787.1:CDS
AAACCAGGTTCACCAATACC+AGG 0.622204 2:+9852361 None:intergenic
CACGGTCGTTTAAGCCTGAG+AGG 0.624256 2:+9853656 None:intergenic
GCTTTAACATACTTCGCCAA+GGG 0.624647 2:+9854259 None:intergenic
GTAATTAGAAGATTGATGGT+AGG 0.628481 2:+9852429 None:intergenic
CTGCGCCTGCATTAAAGCAG+CGG 0.630823 2:+9853188 None:intergenic
TCTTGGAAGACTCATAAGGT+GGG 0.631001 2:+9852778 None:intergenic
AAGGGTATGACAAATTCGGA+TGG 0.631171 2:+9854277 None:intergenic
ACAAACTCAACAGCAAGTGG+TGG 0.632664 2:-9853004 MsaT006787.1:CDS
CTACTGCACTTCAGGACATG+AGG 0.633304 2:-9853287 MsaT006787.1:CDS
CAACGAAGAATCAGCACCTG+TGG 0.634227 2:+9852399 None:intergenic
CTTGCATACCCAGGAAAACG+GGG 0.635833 2:-9852338 MsaT006787.1:CDS
CTCTCAGGCTTAAACGACCG+TGG 0.637655 2:-9853655 MsaT006787.1:CDS
AGAAGATTGATGGTAGGACA+TGG 0.637666 2:+9852435 None:intergenic
TCGAACTTTAAGGGGATCAG+TGG 0.638766 2:-9852478 MsaT006787.1:CDS
AACAAACACATACCTTCTTG+GGG 0.638829 2:+9856780 None:intergenic
TGTTTGCTGAAATTGAAGCA+AGG 0.639205 2:+9853230 None:intergenic
CTGCTCAAGAGTTAATAGCA+AGG 0.640223 2:-9855100 MsaT006787.1:CDS
AGGTGGGATGATTCATCATG+CGG 0.640255 2:+9852794 None:intergenic
TTGGCCGTCGAAGCGCATTG+CGG 0.640591 2:-9852719 MsaT006787.1:intron
TAGTGACACAAGCACTCACG+GGG 0.641025 2:-9855269 MsaT006787.1:CDS
AGGAAAATTCACCTTCACAA+AGG 0.641819 2:+9852288 None:intergenic
TCCGGTGGCAGAACTCACAT+TGG 0.642216 2:-9853963 MsaT006787.1:CDS
GGTGGCTGTGTCAACCAACA+AGG 0.642632 2:-9855704 MsaT006787.1:CDS
TTAATAGCAAGGGATTTGCA+TGG 0.646026 2:-9855089 MsaT006787.1:CDS
TCACTTGACAGTACTGAGGA+AGG 0.646608 2:+9854475 None:intergenic
AAACACATACCTTCTTGGGG+TGG 0.651129 2:+9856783 None:intergenic
TGAAGTTGACAAATAAGCTG+AGG 0.655755 2:+9855643 None:intergenic
GTGTGTCATACAGCATTCAG+AGG 0.660186 2:+9855452 None:intergenic
TGAAGCTAACCATGGAACGA+AGG 0.660506 2:+9853620 None:intergenic
GATGCTCCCGAAGAGAGGAG+TGG 0.661943 2:+9852691 None:intergenic
GTGACCTGGATTCTGTCCGG+TGG 0.662152 2:-9853978 MsaT006787.1:CDS
GAACCCATGATACTGTGCAA+AGG 0.670731 2:+9852824 None:intergenic
ACTGCTGGCAAAGAGACAAG+AGG 0.671615 2:+9856179 None:intergenic
GCTGCCAGCCTGATCTCACA+GGG 0.673300 2:+9852068 None:intergenic
ACTGAACAAGACCTTTGTGA+AGG 0.673840 2:-9852299 MsaT006787.1:intron
CTGCTGGCAAAGAGACAAGA+GGG 0.675164 2:+9856180 None:intergenic
GTGTCTCGGAGCCACAACAA+AGG 0.677037 2:+9853426 None:intergenic
CACTCCCTACCAGGAAACGG+TGG 0.679008 2:+9852595 None:intergenic
ACGGTCGTTTAAGCCTGAGA+GGG 0.680503 2:+9853657 None:intergenic
ATTCTCCGTGCCTCGCCGGG+CGG 0.687655 2:-9855245 MsaT006787.1:CDS
AACCGATATGTATCAAGCTG+TGG 0.692664 2:-9853313 MsaT006787.1:CDS
AGAGTTGTGTACTTTCCACA+AGG 0.698507 2:-9856149 MsaT006787.1:CDS
GGTTTGCATATTAAGCATGG+CGG 0.708185 2:+9853332 None:intergenic
TCAAGTGATAGTATGCACTT+GGG 0.709086 2:-9854460 MsaT006787.1:CDS
ACTATCACTTGACAGTACTG+AGG 0.709379 2:+9854471 None:intergenic
TGCTGGTTATGATGACGGAG+TGG 0.738797 2:+9853033 None:intergenic
TGGAACGAAGGCAATCCAGG+AGG 0.743475 2:+9853632 None:intergenic
TTTGTGGTCTGCTAGCACGG+CGG 0.758571 2:+9854506 None:intergenic
AGTGACACAAGCACTCACGG+GGG 0.846522 2:-9855268 MsaT006787.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 9852083 9856835 9852083 ID=MsaG006787
Chr2 mRNA 9852083 9856835 9852083 ID=MsaT006787.1;Parent=MsaG006787
Chr2 exon 9852083 9852202 9852083 ID=MsaT006787.1.exon15;Parent=MsaT006787.1
Chr2 CDS 9852083 9852202 9852083 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9852300 9852728 9852300 ID=MsaT006787.1.exon14;Parent=MsaT006787.1
Chr2 CDS 9852300 9852728 9852300 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9852760 9852893 9852760 ID=MsaT006787.1.exon13;Parent=MsaT006787.1
Chr2 CDS 9852760 9852893 9852760 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9852983 9853480 9852983 ID=MsaT006787.1.exon12;Parent=MsaT006787.1
Chr2 CDS 9852983 9853480 9852983 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9853630 9853777 9853630 ID=MsaT006787.1.exon11;Parent=MsaT006787.1
Chr2 CDS 9853630 9853777 9853630 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9853951 9854026 9853951 ID=MsaT006787.1.exon10;Parent=MsaT006787.1
Chr2 CDS 9853951 9854026 9853951 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9854184 9854306 9854184 ID=MsaT006787.1.exon9;Parent=MsaT006787.1
Chr2 CDS 9854184 9854306 9854184 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9854394 9854558 9854394 ID=MsaT006787.1.exon8;Parent=MsaT006787.1
Chr2 CDS 9854394 9854558 9854394 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9854838 9854923 9854838 ID=MsaT006787.1.exon7;Parent=MsaT006787.1
Chr2 CDS 9854838 9854923 9854838 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9855055 9855141 9855055 ID=MsaT006787.1.exon6;Parent=MsaT006787.1
Chr2 CDS 9855055 9855141 9855055 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9855222 9855374 9855222 ID=MsaT006787.1.exon5;Parent=MsaT006787.1
Chr2 CDS 9855222 9855374 9855222 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9855463 9855486 9855463 ID=MsaT006787.1.exon4;Parent=MsaT006787.1
Chr2 CDS 9855463 9855486 9855463 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9855627 9855725 9855627 ID=MsaT006787.1.exon3;Parent=MsaT006787.1
Chr2 CDS 9855627 9855725 9855627 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9856136 9856236 9856136 ID=MsaT006787.1.exon2;Parent=MsaT006787.1
Chr2 CDS 9856136 9856236 9856136 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Chr2 exon 9856793 9856835 9856793 ID=MsaT006787.1.exon1;Parent=MsaT006787.1
Chr2 CDS 9856793 9856835 9856793 ID=cds.MsaT006787.1;Parent=MsaT006787.1
Gene Sequence

>MsaT006787.1

ATGAGACTCTCTTCAGCTGGTTTTAGTCCTCCACCCCAAGAAGTTCTAGATTCAGAACTTTGGCATGCATGTGCTGGCCCTCTTGTCTCTTTGCCAGCAGTTGGAAGTAGAGTTGTGTACTTTCCACAAGGTCACAGTGAACAAGTGGCTGTGTCAACCAACAAGGAAGTCGATGCACATATCCCAAACTATCCAAGCTTACCTCCTCAGCTTATTTGTCAACTTCATAATTTGACTATGCATATGACCTTGCAACCTCTGAATGCTCAAGAGCAAAAGAGGCACCTTCCAGCAGAGCTGGGAACTCCAAGTAAACAGCCGACAAACTACTTTTGCAAGACCTTGACTGCTAGTGACACAAGCACTCACGGGGGATTCTCCGTGCCTCGCCGGGCGGCTGAAAAAGTGTTTCCTCCTTTGGATTTCTCCCAACAGCCTCCTGCTCAAGAGTTAATAGCAAGGGATTTGCATGGTAACGAATGGAAATTCAGACATATCTTCGAGCGACCCAAAAGGCATCTACTTACAACTGGATGGAGTGTTTTTGTGAGTGCTAAAAGACTTGTTGCTGGTGATTCTGTGCTGTTTATCTGGAATGAAAAGAACCAATTGCTTCTTGGTATCCGCCGTGCTAGCAGACCACAAACTGTGATGCCTTCCTCAGTACTGTCAAGTGATAGTATGCACTTGGGACTTCTTGCTGCAGCAGCTCATGCGGCTGCAACCAATAGCCGTTTCACTATTTTCTATAACCCACGTGCGTGTCCATCCGAATTTGTCATACCCTTGGCGAAGTATGTTAAAGCTGTGTATCATACTCGAGTTTCAGTTGGTATGCGTTTCAGGATGCTCTTTGAAACAGAGGAGTCTAGTGTGCGACTATACATGGGTCCAATAACTGGCATTTGTGACCTGGATTCTGTCCGGTGGCAGAACTCACATTGGCGCTCGGTAAAGGTTGGTTGGGATGAATCCACTGCCGGAGAAAGGCAACCTAGAGTGTCTTTGTGGGAAATCGAGCCATTAACAACATTTCCTATGTATCCGTCACCATTCCCTCTCAGGCTTAAACGACCGTGGCCTCCTGGATTGCCTTCGTTCCATGGCATGAAAGATGATGATTTTGGCATGAATTCTCCTTTGTTGTGGCTCCGAGACACGGATAGAGGGCTTCAATCTCTTAATTATCAAGGGATTGGGGTTAATTCTTGGATGCAGCCAAGGTTTGATCCCGCCATGCTTAATATGCAAACCGATATGTATCAAGCTGTGGCTGCTACTGCACTTCAGGACATGAGGACTGTAGTAGATCCTTCCAAACAGCTTCTGGGTTCCTTGCTTCAATTTCAGCAAACACCGAACTTCCCGAACAGGACCGCTGCTTTAATGCAGGCGCAGATGTTGCAACAGTCGCAGCCTCAACAGGCCTTTCAAAATAATAATCAGGAGAGTCAAAATCTGTCGCAATCTCAGCCACAAGCTCAGACTAATCCTCAACAGCATCTACAGCATCAGCACTCATTCAATAATCCACTCCGTCATCATAACCAGCAGCAACAACAAACTCAACAGCAAGTGGTGGATAATAATCAGCAGATTTCTGGAGAGTTTTCTGATTCAAATGTGAACTCTTCAACTACTATTGTTTCTCCTTTGCACAGTATCATGGGTTCATCGTTTCCGCATGATGAATCATCCCACCTTATGAGTCTTCCAAGAACAAGCTCTTGGGTTCCTCGCATTGCGGTGGATCCACTCCTCTCTTCGGGAGCATCTCAATGTATTCTGCCTCAGGTGGAGCAGTTAGGGCAGGCACAGAATTCATTGTCTCAAAATGTTATTACATTACCACCGTTTCCTGGTAGGGAGTGCCCAATAGACCAAGAAGGGAGCAATGATCCGCAAAGTAATCTTTTATTCGGTGTCAATATAGATCCCTCATCTCTTCTAATGCATAATGGGATGTCGAACTTTAAGGGGATCAGTGGCAACAACAATGATTCCTCAACCATGTCCTACCATCAATCTTCTAATTACATGAACACCACAGGTGCTGATTCTTCGTTGAATCATGGAGTAACGCCTGGTATTGGTGAACCTGGTTTCTTGCATACCCAGGAAAACGGGGATCAAGGAAACAACCCACTGAACAAGACCTTTGTGAAGGTTTATAAATCAGGGTCCTTGGAAGATCGTTGGATATCACTAAATTCAGCAGCTACAATGAGCTGCGTAGTGAGCTTCGCCGAATGTTCGGACTGGAAGGCGAGTTGGAGGACCCTGTGA
Protein sequence

>MsaT006787.1

MRLSSAGFSPPPQEVLDSELWHACAGPLVSLPAVGSRVVYFPQGHSEQVAVSTNKEVDAHIPNYPSLPPQLICQLHNLTMHMTLQPLNAQEQKRHLPAELGTPSKQPTNYFCKTLTASDTSTHGGFSVPRRAAEKVFPPLDFSQQPPAQELIARDLHGNEWKFRHIFERPKRHLLTTGWSVFVSAKRLVAGDSVLFIWNEKNQLLLGIRRASRPQTVMPSSVLSSDSMHLGLLAAAAHAAATNSRFTIFYNPRACPSEFVIPLAKYVKAVYHTRVSVGMRFRMLFETEESSVRLYMGPITGICDLDSVRWQNSHWRSVKVGWDESTAGERQPRVSLWEIEPLTTFPMYPSPFPLRLKRPWPPGLPSFHGMKDDDFGMNSPLLWLRDTDRGLQSLNYQGIGVNSWMQPRFDPAMLNMQTDMYQAVAATALQDMRTVVDPSKQLLGSLLQFQQTPNFPNRTAALMQAQMLQQSQPQQAFQNNNQESQNLSQSQPQAQTNPQQHLQHQHSFNNPLRHHNQQQQQTQQQVVDNNQQISGEFSDSNVNSSTTIVSPLHSIMGSSFPHDESSHLMSLPRTSSWVPRIAVDPLLSSGASQCILPQVEQLGQAQNSLSQNVITLPPFPGRECPIDQEGSNDPQSNLLFGVNIDPSSLLMHNGMSNFKGISGNNNDSSTMSYHQSSNYMNTTGADSSLNHGVTPGIGEPGFLHTQENGDQGNNPLNKTFVKVYKSGSLEDRWISLNSAATMSCVVSFAECSDWKASWRTL*