AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006851

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006851 MtrunA17_Chr2g0286191 89.744 117 12 0 22 138 249 365 4.70e-71 218
MsaG006851 MtrunA17_Chr2g0286221 81.034 116 22 0 22 137 59 174 1.48e-62 190
MsaG006851 MtrunA17_Chr2g0295761 52.174 115 54 1 22 135 278 392 1.88e-33 121
MsaG006851 MtrunA17_Chr2g0295731 48.120 133 68 1 5 136 249 381 7.31e-33 119
MsaG006851 MtrunA17_Chr4g0067121 51.282 117 56 1 22 137 269 385 5.28e-32 117
MsaG006851 MtrunA17_Chr2g0296711 49.219 128 64 1 22 148 275 402 1.27e-30 114
MsaG006851 MtrunA17_Chr2g0296151 51.724 116 55 1 22 136 259 374 3.39e-30 112
MsaG006851 MtrunA17_Chr2g0296121 50.862 116 56 1 22 136 345 460 7.43e-30 113
MsaG006851 MtrunA17_Chr2g0296751 52.679 112 52 1 22 132 18 129 9.01e-30 106
MsaG006851 MtrunA17_Chr2g0296131 45.690 116 62 1 22 136 51 166 4.28e-29 105
MsaG006851 MtrunA17_Chr2g0296291 46.875 128 67 1 22 148 287 414 4.95e-29 110
MsaG006851 MtrunA17_Chr2g0296161 47.414 116 60 1 22 136 47 162 6.75e-29 104
MsaG006851 MtrunA17_Chr2g0296701 46.094 128 68 1 22 148 45 172 3.92e-28 102
MsaG006851 MtrunA17_Chr2g0295741 46.552 116 61 1 22 136 265 380 4.23e-28 107
MsaG006851 MtrunA17_Chr2g0296211 50.893 112 54 1 22 132 247 358 1.28e-27 105
MsaG006851 MtrunA17_Chr2g0296741 44.531 128 70 1 22 148 292 419 1.53e-27 106
MsaG006851 MtrunA17_Chr2g0296251 51.786 112 53 1 22 132 287 398 2.72e-27 105
MsaG006851 MtrunA17_Chr2g0296301 47.748 111 57 1 22 131 255 365 7.33e-27 103
MsaG006851 MtrunA17_Chr2g0296111 50.000 118 53 3 22 136 260 374 1.91e-26 102
MsaG006851 MtrunA17_Chr2g0296721 44.186 129 71 1 22 149 271 399 2.22e-26 102
MsaG006851 MtrunA17_Chr2g0296171 50.000 114 56 1 22 134 284 397 3.06e-26 102
MsaG006851 MtrunA17_Chr2g0296551 49.107 112 56 1 22 132 73 184 4.25e-26 98.2
MsaG006851 MtrunA17_Chr2g0296221 50.893 112 54 1 22 132 303 414 1.48e-25 100
MsaG006851 MtrunA17_Chr2g0315731 43.750 128 71 1 22 148 292 419 3.32e-25 99.8
MsaG006851 MtrunA17_Chr2g0296231 50.000 112 53 2 22 132 271 380 6.18e-24 96.3
MsaG006851 MtrunA17_Chr2g0296191 43.750 112 62 1 22 132 399 510 3.11e-23 95.1
MsaG006851 MtrunA17_Chr2g0296191 47.706 109 56 1 22 129 886 994 2.13e-22 92.8
MsaG006851 MtrunA17_Chr4g0067141 45.872 109 58 1 22 129 261 369 9.96e-22 89.7
MsaG006851 MtrunA17_Chr2g0295721 40.659 91 54 0 4 94 29 119 2.61e-17 73.2
MsaG006851 MtrunA17_Chr4g0067131 45.679 81 43 1 49 128 1 81 7.32e-17 71.2
MsaG006851 MtrunA17_Chr4g0067671 39.362 94 56 1 23 115 262 355 5.89e-15 70.9
MsaG006851 MtrunA17_Chr4g0001831 34.513 113 73 1 22 133 259 371 4.17e-14 68.6
MsaG006851 MtrunA17_Chr2g0296181 41.071 112 39 2 22 132 272 357 2.85e-13 66.2
MsaG006851 MtrunA17_Chr8g0377781 37.963 108 65 2 20 126 262 368 8.28e-13 65.1
MsaG006851 MtrunA17_Chr4g0067681 48.333 60 31 0 38 97 1 60 1.58e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006851 AT5G07900.1 43.119 109 61 1 22 129 291 399 5.07e-21 88.6
MsaG006851 AT1G21150.2 32.456 114 75 1 22 133 341 454 1.07e-14 70.9
MsaG006851 AT1G21150.3 32.456 114 75 1 22 133 241 354 1.09e-14 70.5
MsaG006851 AT1G21150.1 32.456 114 75 1 22 133 277 390 1.40e-14 70.5
MsaG006851 AT1G61960.1 32.673 101 64 1 33 129 351 451 5.40e-13 65.9
MsaG006851 AT1G61990.2 35.644 101 61 2 33 129 308 408 1.79e-11 61.6
MsaG006851 AT1G61990.1 35.644 101 61 2 33 129 308 408 1.79e-11 61.6
MsaG006851 AT1G62110.1 33.663 101 61 2 20 115 340 439 3.06e-11 60.8

Find 29 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAAGAAGTATTGGATATT+TGG 0.165459 2:+10726222 None:intergenic
TTCCTTTCCCATACGGGTTT+TGG 0.211977 2:+10726398 None:intergenic
AATACTTCTTATGCTGAGTT+TGG 0.261332 2:-10726212 MsaT006851.1:CDS
TCGATCCAAAGGTTTGATTA+AGG 0.285728 2:-10726149 MsaT006851.1:CDS
AGATGTTTCAACTTTCAATA+AGG 0.340961 2:-10726014 MsaT006851.1:CDS
AGCATCCTTAATCAAACCTT+TGG 0.342269 2:+10726144 None:intergenic
GATTAATGCGTATAAGAAAT+GGG 0.369032 2:-10726375 MsaT006851.1:CDS
AGATTAATGCGTATAAGAAA+TGG 0.381339 2:-10726376 MsaT006851.1:CDS
TGCGTATAAGAAATGGGGTT+TGG 0.406555 2:-10726369 MsaT006851.1:CDS
AAACTCAGCATAAGAAGTAT+TGG 0.453268 2:+10726214 None:intergenic
TCAGAGACTTTGAAAGGTGC+AGG 0.454054 2:+10726115 None:intergenic
TTGAAATTTCTTCGATCCAA+AGG 0.475136 2:-10726160 MsaT006851.1:CDS
TTAATCTTCCTTTCCCATAC+GGG 0.481781 2:+10726392 None:intergenic
ATTAATCTTCCTTTCCCATA+CGG 0.500717 2:+10726391 None:intergenic
AGATTAGTCCAAAACCCGTA+TGG 0.528933 2:-10726406 MsaT006851.1:CDS
GATTAGTCCAAAACCCGTAT+GGG 0.549456 2:-10726405 MsaT006851.1:CDS
GTCCAAAACCCGTATGGGAA+AGG 0.554208 2:-10726400 MsaT006851.1:CDS
CAATCTTCGAAGCAACGCAA+AGG 0.555930 2:-10725980 MsaT006851.1:CDS
GAAGTATCCATGGTGTATGT+TGG 0.562557 2:-10726317 MsaT006851.1:CDS
ATGGTGTATGTTGGCATCTG+TGG 0.563796 2:-10726308 MsaT006851.1:CDS
ATTAATGCGTATAAGAAATG+GGG 0.571047 2:-10726374 MsaT006851.1:CDS
AATCTTCGAAGCAACGCAAA+GGG 0.578629 2:-10725979 MsaT006851.1:CDS
CAGAGACTTTGAAAGGTGCA+GGG 0.587463 2:+10726116 None:intergenic
GATTCTAAGTTGCAGTGACA+TGG 0.589689 2:+10725942 None:intergenic
AATTTCAACACAAAAGCCCT+AGG 0.603574 2:+10726175 None:intergenic
AGAGACTTTGAAAGGTGCAG+GGG 0.631390 2:+10726117 None:intergenic
AGATCCTCAGAGACTTTGAA+AGG 0.638683 2:+10726109 None:intergenic
TGCACCTTTCAAAGTCTCTG+AGG 0.647402 2:-10726113 MsaT006851.1:CDS
ACAGATGCCAACATACACCA+TGG 0.670800 2:+10726310 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 10725946 10726434 10725946 ID=MsaG006851
Chr2 mRNA 10725946 10726434 10725946 ID=MsaT006851.1;Parent=MsaG006851
Chr2 exon 10725946 10726434 10725946 ID=MsaT006851.1.exon1;Parent=MsaT006851.1
Chr2 CDS 10725946 10726434 10725946 ID=cds.MsaT006851.1;Parent=MsaT006851.1
Gene Sequence

>MsaT006851.1

ATGCTAAGATTAGTCCAAAACCCGTATGGGAAAGGAAGATTAATGCGTATAAGAAATGGGGTTTGGTCTGATGAAATTATTGCCTCAGCATTTTTGAAGTATCCATGGTGTATGTTGGCATCTGTGGAAAAAATTGAAGCAGTGATGCAGTTTTTTGTCAATCACATGGATTGGGAGTCTAATGTGCTTGCCAAATATCCAATACTTCTTATGCTGAGTTTGGAAAAAAGGGTTATTCCTAGGGCTTTTGTGTTGAAATTTCTTCGATCCAAAGGTTTGATTAAGGATGCTAAATCCCCTGCACCTTTCAAAGTCTCTGAGGATCTGTTTTTGAAGAGATTTGTGACTTGTTTCGAAGAAGAAGCATCTCAGCTGTTGAAGTTGTATGAAGAGAAAAGAGATGTTTCAACTTTCAATAAGGTTTCGCAACTGCAATCTTCGAAGCAACGCAAAGGGTCTCTGCTACACGACCATGTCACTGCAACTTAG
Protein sequence

>MsaT006851.1

MLRLVQNPYGKGRLMRIRNGVWSDEIIASAFLKYPWCMLASVEKIEAVMQFFVNHMDWESNVLAKYPILLMLSLEKRVIPRAFVLKFLRSKGLIKDAKSPAPFKVSEDLFLKRFVTCFEEEASQLLKLYEEKRDVSTFNKVSQLQSSKQRKGSLLHDHVTAT*