AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006877


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006877 MtrunA17_Chr2g0286441 81.723 383 33 3 1 347 1 382 0.0 620
MsaG006877 MtrunA17_Chr1g0196071 58.133 375 115 6 11 347 21 391 1.11e-148 425
MsaG006877 MtrunA17_Chr1g0201891 53.651 315 104 4 34 306 48 362 2.33e-113 336
MsaG006877 MtrunA17_Chr2g0278511 52.174 322 107 5 34 310 49 368 1.89e-112 333
MsaG006877 MtrunA17_Chr2g0315641 54.098 305 130 4 16 313 20 321 3.72e-111 328
MsaG006877 MtrunA17_Chr5g0404321 51.911 314 114 2 32 308 48 361 4.41e-111 330
MsaG006877 MtrunA17_Chr8g0373001 52.885 312 112 2 32 308 46 357 8.58e-108 321
MsaG006877 MtrunA17_Chr1g0155301 54.125 303 111 3 39 313 70 372 2.53e-106 318
MsaG006877 MtrunA17_Chr2g0315651 48.592 284 134 5 39 313 37 317 2.02e-83 257
MsaG006877 MtrunA17_Chr8g0373361 37.396 361 171 10 32 347 51 401 4.90e-67 216
MsaG006877 MtrunA17_Chr1g0156151 58.904 146 56 1 54 195 66 211 1.37e-53 176
MsaG006877 MtrunA17_Chr8g0373331 35.570 298 124 7 32 306 45 297 1.36e-48 167
MsaG006877 MtrunA17_Chr8g0373341 37.631 287 98 9 39 307 50 273 1.08e-38 140
MsaG006877 MtrunA17_Chr8g0373351 34.413 247 87 8 87 308 43 239 7.21e-26 105
MsaG006877 MtrunA17_Chr3g0091401 42.268 97 50 1 82 178 9 99 2.09e-15 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006877 AT2G47900.2 64.134 329 81 4 12 308 30 353 1.78e-152 435
MsaG006877 AT2G47900.1 64.134 329 81 4 12 308 30 353 1.78e-152 435
MsaG006877 AT2G47900.3 63.939 330 81 4 12 308 30 354 2.15e-152 435
MsaG006877 AT3G06380.1 53.050 377 134 8 4 347 3 369 5.35e-131 379
MsaG006877 AT5G18680.1 52.632 380 140 8 4 347 3 378 9.35e-129 374
MsaG006877 AT2G18280.2 50.867 346 136 6 32 347 41 382 2.07e-110 328
MsaG006877 AT2G18280.1 50.867 346 136 6 32 347 41 382 2.07e-110 328
MsaG006877 AT1G76900.2 48.725 353 115 7 17 308 37 384 4.09e-109 327
MsaG006877 AT1G76900.1 48.725 353 115 7 17 308 37 384 4.09e-109 327
MsaG006877 AT1G76900.3 48.725 353 115 7 17 308 37 384 4.09e-109 327
MsaG006877 AT1G43640.1 47.130 331 139 5 14 312 34 360 1.80e-106 319
MsaG006877 AT1G53320.1 49.379 322 152 2 3 313 8 329 2.62e-104 311
MsaG006877 AT1G47270.3 47.429 350 141 7 32 347 37 377 1.89e-102 307
MsaG006877 AT1G47270.1 47.429 350 141 7 32 347 62 402 4.26e-102 307
MsaG006877 AT1G47270.2 47.429 350 141 7 32 347 61 401 4.92e-102 307
MsaG006877 AT1G43640.2 48.973 292 117 4 53 312 3 294 2.16e-99 298
MsaG006877 AT5G18680.2 52.941 289 98 6 93 347 2 286 3.74e-95 285
MsaG006877 AT3G06380.2 53.957 278 91 6 102 347 5 277 4.92e-93 280
MsaG006877 AT1G25280.1 44.063 379 140 7 4 310 3 381 1.98e-90 278
MsaG006877 AT1G25280.3 43.350 203 65 3 158 310 1 203 4.49e-44 153
MsaG006877 AT1G25280.2 43.350 203 65 3 158 310 1 203 4.49e-44 153
MsaG006877 AT1G61940.2 68.605 86 27 0 42 127 1 86 2.68e-36 132
MsaG006877 AT1G61940.1 70.492 61 18 0 42 102 1 61 2.03e-22 95.1

Find 93 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGATCATAGCTGAAATTTC+AGG 0.195471 2:+11029791 None:intergenic
GACAAGATTATTCTGCAATT+TGG 0.212541 2:-11026744 MsaT006877.1:CDS
ATACCCTATTTCGGCATTTC+AGG 0.216986 2:-11026685 MsaT006877.1:CDS
AATGCCTGAAATGCCGAAAT+AGG 0.239475 2:+11026681 None:intergenic
TGGCGGTTGGCGACAGTTTA+CGG 0.281341 2:-11029970 MsaT006877.1:CDS
GAAAACCTGTTTAACAGAAA+TGG 0.297610 2:+11029763 None:intergenic
GCTGGTGGCTTCCGAGAAAA+TGG 0.312186 2:-11026785 MsaT006877.1:CDS
AAAACCTGTTTAACAGAAAT+GGG 0.345752 2:+11029764 None:intergenic
ATTCTGCAATTTGGAAAAGT+TGG 0.358632 2:-11026735 MsaT006877.1:CDS
TTTCCCCATTTCTGTTAAAC+AGG 0.366547 2:-11029768 MsaT006877.1:intron
GGATTTCCGATACCCTATTT+CGG 0.372729 2:-11026694 MsaT006877.1:CDS
AGTAGTGGTGGTGGCGGAGA+AGG 0.373376 2:-11030002 MsaT006877.1:CDS
TCCTTAAGATCAAATTTCCT+TGG 0.391394 2:-11027682 MsaT006877.1:intron
AACATAGTTGTTGCTTCCCC+TGG 0.398674 2:+11027808 None:intergenic
TAGTTGCCTGTTGGGACCTT+GGG 0.403395 2:+11027562 None:intergenic
CAGAATAATCTTGTCGTGTT+CGG 0.408007 2:+11026752 None:intergenic
ACAGTTTACGGCAGAGTTGT+TGG 0.415866 2:-11029958 MsaT006877.1:CDS
TTCTGCAATTTGGAAAAGTT+GGG 0.417811 2:-11026734 MsaT006877.1:CDS
TCTCTATCCGGACCTCTTGT+TGG 0.419329 2:-11026928 MsaT006877.1:CDS
TGATGACGGGAAGTTCCTTC+TGG 0.420704 2:-11027897 MsaT006877.1:CDS
ATAGTTGCCTGTTGGGACCT+TGG 0.425411 2:+11027561 None:intergenic
TTACTAAAAGTCGTTCTACT+AGG 0.428715 2:-11027609 MsaT006877.1:CDS
CCACTGGATAGTTGCCTGTT+GGG 0.432338 2:+11027554 None:intergenic
CAGTTTACGGCAGAGTTGTT+GGG 0.438166 2:-11029957 MsaT006877.1:CDS
ATGCCTGAAATGCCGAAATA+GGG 0.443678 2:+11026682 None:intergenic
AAGAACGTTGTTGCGTGTGC+TGG 0.445764 2:-11029860 MsaT006877.1:CDS
TTGCTCCTGCATGAGGTGGC+TGG 0.453181 2:+11027635 None:intergenic
GGCATGAGCAGCTGCAGTGT+TGG 0.476628 2:-11026858 MsaT006877.1:CDS
GGGGAAGCAACAACTATGTT+GGG 0.477342 2:-11027805 MsaT006877.1:CDS
GTTTCACCCAAGGTCCCAAC+AGG 0.477369 2:-11027568 MsaT006877.1:CDS
TGCGCTTTATAAAGCATTGA+AGG 0.484376 2:+11028082 None:intergenic
AGGAGATTCTCCCTTGGACC+AGG 0.489762 2:+11028102 None:intergenic
AATAATATGTTTGAGTGGAC+CGG 0.492380 2:+11028058 None:intergenic
TCTTCTCCGAATTGAGGCCT+CGG 0.497256 2:-11029903 MsaT006877.1:CDS
AATGCTTTATAAAGCGCAAC+CGG 0.499079 2:-11028077 MsaT006877.1:CDS
TGAAGTTCTTCTCCGAATTG+AGG 0.501294 2:-11029909 MsaT006877.1:CDS
TGGGAAGGATTTGTTTACAA+TGG 0.502527 2:-11026715 MsaT006877.1:CDS
GACGGCCAGCCACCTCATGC+AGG 0.508409 2:-11027640 MsaT006877.1:CDS
AGGGGAAGCAACAACTATGT+TGG 0.508852 2:-11027806 MsaT006877.1:CDS
TTCCGAGAAAATGGAACAGC+TGG 0.510258 2:-11026776 MsaT006877.1:CDS
TTTATCGCCAACAAGAGGTC+CGG 0.512577 2:+11026921 None:intergenic
TAAAAGTCGTTCTACTAGGT+TGG 0.514151 2:-11027605 MsaT006877.1:CDS
TGCAGCTGCTCATGCCACCT+CGG 0.519909 2:+11026865 None:intergenic
AATTAAATGTGCTAGGCTCT+AGG 0.520129 2:-11027516 MsaT006877.1:intron
ACAACTATGTTGGGAAATTG+AGG 0.521031 2:-11027796 MsaT006877.1:intron
TAGACGCAGATGATATGTCC+AGG 0.522084 2:-11027826 MsaT006877.1:CDS
ATGACACAGTGCATTCTCCT+TGG 0.528561 2:+11027082 None:intergenic
TAGGTTGGTGAATCTAAAGC+AGG 0.531070 2:-11027590 MsaT006877.1:CDS
TCATGTCTTCGATTATGTTG+CGG 0.535725 2:+11030041 None:intergenic
TTCGATTGAAGAGGATGGAA+TGG 0.537253 2:+11027054 None:intergenic
AGATAGTGAACTTTGTTCCA+AGG 0.538026 2:+11027665 None:intergenic
GTGGGAGAGAACTCTCTATC+CGG 0.538392 2:-11026940 MsaT006877.1:intron
TGTCCTCCGAGGCCTCAATT+CGG 0.540412 2:+11029897 None:intergenic
ATTCAGCGCTAGGTGATGAC+GGG 0.542238 2:-11027910 MsaT006877.1:intron
AATTCAGCGCTAGGTGATGA+CGG 0.546541 2:-11027911 MsaT006877.1:intron
AAGGAGAATGCACTGTGTCA+TGG 0.546764 2:-11027080 MsaT006877.1:CDS
ATGATATAATCTGTGCATGT+AGG 0.547626 2:+11027854 None:intergenic
GCCACTGGATAGTTGCCTGT+TGG 0.548446 2:+11027553 None:intergenic
TCATATGAGATGTGAGCCAC+TGG 0.549162 2:+11027538 None:intergenic
GCAATTTGGAAAAGTTGGGA+AGG 0.553649 2:-11026730 MsaT006877.1:CDS
ACTTCACGCAGAAGCTCTTG+TGG 0.562675 2:+11029926 None:intergenic
CCATCCTCTTCAATCGAACC+AGG 0.562739 2:-11027049 MsaT006877.1:intron
CATTGAAGGAGATTCTCCCT+TGG 0.563702 2:+11028096 None:intergenic
CACGCAACAACGTTCTTCCT+CGG 0.565317 2:+11029866 None:intergenic
GTGCTGGAGTGTGTAGGAGT+TGG 0.567870 2:-11029844 MsaT006877.1:CDS
GGCGGAGAAGGAAGAGAAAG+TGG 0.570148 2:-11029990 MsaT006877.1:CDS
TCATATGAATTAAATGTGCT+AGG 0.570586 2:-11027523 MsaT006877.1:CDS
CCTGGTTCGATTGAAGAGGA+TGG 0.573458 2:+11027049 None:intergenic
TACATTTGCGTGCAGCCAGA+AGG 0.580300 2:+11027882 None:intergenic
TGGACGGGTGACAATTGCCT+CGG 0.581243 2:-11026821 MsaT006877.1:CDS
AAGGAAGAGAAAGTGGCGGT+TGG 0.582497 2:-11029983 MsaT006877.1:CDS
ATCGCAAAGAGTAGTGGTGG+TGG 0.589679 2:-11030011 MsaT006877.1:CDS
TTGCGTGTGCTGGAGTGTGT+AGG 0.591079 2:-11029850 MsaT006877.1:CDS
CGGAGGACACGTGGCCGCCG+AGG 0.594437 2:-11029883 MsaT006877.1:CDS
GCAAAGAGTAGTGGTGGTGG+CGG 0.599034 2:-11030008 MsaT006877.1:CDS
CTTGTTGGCGATAAAAGAGA+TGG 0.602960 2:-11026913 MsaT006877.1:CDS
GAAATGCCGAAATAGGGTAT+CGG 0.603573 2:+11026688 None:intergenic
TTGAGGCCTCGGAGGACACG+TGG 0.607574 2:-11029892 MsaT006877.1:CDS
GCCACCTGGTTCGATTGAAG+AGG 0.610396 2:+11027045 None:intergenic
AAACAAATAATATGTTTGAG+TGG 0.611039 2:+11028053 None:intergenic
AGACGCAGATGATATGTCCA+GGG 0.615218 2:-11027825 MsaT006877.1:CDS
ATCAAGATCGCAAAGAGTAG+TGG 0.616284 2:-11030017 MsaT006877.1:CDS
ACAAAGTTCACTATCTATGA+CGG 0.627271 2:-11027658 MsaT006877.1:CDS
TCTAAAGCAGGTTTCACCCA+AGG 0.631576 2:-11027578 MsaT006877.1:CDS
TCTCCGAATTGAGGCCTCGG+AGG 0.639401 2:-11029900 MsaT006877.1:CDS
AAGATCGCAAAGAGTAGTGG+TGG 0.644592 2:-11030014 MsaT006877.1:CDS
CGGCGGCCACGTGTCCTCCG+AGG 0.663112 2:+11029886 None:intergenic
TCTTGGTATCAAAGCTGCTG+AGG 0.664183 2:+11026649 None:intergenic
CCCAACAGGCAACTATCCAG+TGG 0.669323 2:-11027554 MsaT006877.1:CDS
AAACCTGTTTAACAGAAATG+GGG 0.674456 2:+11029765 None:intergenic
GACGCAGATGATATGTCCAG+GGG 0.717675 2:-11027824 MsaT006877.1:CDS
GCAACAACGTTCTTCCTCGG+CGG 0.717767 2:+11029869 None:intergenic
GGAGAAGGAAGAGAAAGTGG+CGG 0.735942 2:-11029987 MsaT006877.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 11026657 11030074 11026657 ID=MsaG006877
Chr2 mRNA 11026657 11030074 11026657 ID=MsaT006877.1;Parent=MsaG006877
Chr2 exon 11026657 11026955 11026657 ID=MsaT006877.1.exon6;Parent=MsaT006877.1
Chr2 CDS 11026657 11026955 11026657 ID=cds.MsaT006877.1;Parent=MsaT006877.1
Chr2 exon 11027050 11027108 11027050 ID=MsaT006877.1.exon5;Parent=MsaT006877.1
Chr2 CDS 11027050 11027108 11027050 ID=cds.MsaT006877.1;Parent=MsaT006877.1
Chr2 exon 11027517 11027696 11027517 ID=MsaT006877.1.exon4;Parent=MsaT006877.1
Chr2 CDS 11027517 11027696 11027517 ID=cds.MsaT006877.1;Parent=MsaT006877.1
Chr2 exon 11027797 11027926 11027797 ID=MsaT006877.1.exon3;Parent=MsaT006877.1
Chr2 CDS 11027797 11027926 11027797 ID=cds.MsaT006877.1;Parent=MsaT006877.1
Chr2 exon 11028037 11028124 11028037 ID=MsaT006877.1.exon2;Parent=MsaT006877.1
Chr2 CDS 11028037 11028124 11028037 ID=cds.MsaT006877.1;Parent=MsaT006877.1
Chr2 exon 11029769 11030074 11029769 ID=MsaT006877.1.exon1;Parent=MsaT006877.1
Chr2 CDS 11029769 11030074 11029769 ID=cds.MsaT006877.1;Parent=MsaT006877.1
Gene Sequence

>MsaT006877.1

ATGGCTATAATCCGCAACATAATCGAAGACATGAGATCAAGATCGCAAAGAGTAGTGGTGGTGGCGGAGAAGGAAGAGAAAGTGGCGGTTGGCGACAGTTTACGGCAGAGTTGTTGGGCTAACATGCCACAAGAGCTTCTGCGTGAAGTTCTTCTCCGAATTGAGGCCTCGGAGGACACGTGGCCGCCGAGGAAGAACGTTGTTGCGTGTGCTGGAGTGTGTAGGAGTTGGAGAGTTATCACTAAAGAGATTGTTAAAAAACCTGAAATTTCAGCTATGATCACTTTCCCCATTTCTGTTAAACAGCCTGGTCCAAGGGAGAATCTCCTTCAATGCTTTATAAAGCGCAACCGGTCCACTCAAACATATTATTTGTTTCTCAGTTTAACTAGTTCGCTAGGTGATGACGGGAAGTTCCTTCTGGCTGCACGCAAATGTAGACGTCCTACATGCACAGATTATATCATCTCTCTAGACGCAGATGATATGTCCAGGGGAAGCAACAACTATGTTGGGAAATTGAGATCAAATTTCCTTGGAACAAAGTTCACTATCTATGACGGCCAGCCACCTCATGCAGGAGCAAAATTTACTAAAAGTCGTTCTACTAGGTTGGTGAATCTAAAGCAGGTTTCACCCAAGGTCCCAACAGGCAACTATCCAGTGGCTCACATCTCATATGAATTAAATGTGCTAGGCTCTAGGGGGCCAAGGAGAATGCACTGTGTCATGGATTCCATTCCATCCTCTTCAATCGAACCAGAGAACTCTCTATCCGGACCTCTTGTTGGCGATAAAAGAGATGGTATGCTTGTGTTGAAAAACAAGGCTCCGAGGTGGCATGAGCAGCTGCAGTGTTGGTGCTTAAATTTTCATGGACGGGTGACAATTGCCTCGGTTAAAAACTTTCAGCTGGTGGCTTCCGAGAAAATGGAACAGCTGGACCGAACACGACAAGATTATTCTGCAATTTGGAAAAGTTGGGAAGGATTTGTTTACAATGGATTTCCGATACCCTATTTCGGCATTTCAGGCATTTGCAATCTGCCTCAGCAGCTTTGA

Protein sequence

>MsaT006877.1

MAIIRNIIEDMRSRSQRVVVVAEKEEKVAVGDSLRQSCWANMPQELLREVLLRIEASEDTWPPRKNVVACAGVCRSWRVITKEIVKKPEISAMITFPISVKQPGPRENLLQCFIKRNRSTQTYYLFLSLTSSLGDDGKFLLAARKCRRPTCTDYIISLDADDMSRGSNNYVGKLRSNFLGTKFTIYDGQPPHAGAKFTKSRSTRLVNLKQVSPKVPTGNYPVAHISYELNVLGSRGPRRMHCVMDSIPSSSIEPENSLSGPLVGDKRDGMLVLKNKAPRWHEQLQCWCLNFHGRVTIASVKNFQLVASEKMEQLDRTRQDYSAIWKSWEGFVYNGFPIPYFGISGICNLPQQL*