AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006891

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006891 MtrunA17_Chr2g0286661 97.189 747 21 0 1 747 12 758 0.0 1505
MsaG006891 MtrunA17_Chr1g0194121 27.607 326 199 11 270 581 447 749 1.74e-28 122
MsaG006891 MtrunA17_Chr1g0194121 48.315 89 46 0 77 165 132 220 3.19e-21 99.4
MsaG006891 MtrunA17_Chr7g0270541 26.176 340 213 10 273 598 449 764 1.42e-25 113
MsaG006891 MtrunA17_Chr7g0270541 46.809 94 48 1 79 170 130 223 6.81e-22 101
MsaG006891 MtrunA17_Chr1g0162871 29.600 250 161 4 261 502 449 691 2.87e-25 112
MsaG006891 MtrunA17_Chr1g0162871 38.922 167 81 6 29 184 41 197 7.95e-23 104
MsaG006891 MtrunA17_Chr8g0334671 56.250 80 35 0 86 165 181 260 2.15e-24 105
MsaG006891 MtrunA17_Chr2g0302791 25.155 322 213 5 273 583 465 769 9.48e-24 107
MsaG006891 MtrunA17_Chr2g0302791 48.958 96 47 1 79 172 124 219 5.82e-22 101
MsaG006891 MtrunA17_Chr4g0046991 56.410 78 34 0 92 169 73 150 2.67e-23 97.1
MsaG006891 MtrunA17_Chr5g0420051 50.000 92 45 1 79 169 167 258 1.42e-22 101
MsaG006891 MtrunA17_Chr7g0258471 46.512 86 46 0 80 165 130 215 3.18e-22 100
MsaG006891 MtrunA17_Chr3g0122411 37.956 137 82 2 77 211 150 285 3.81e-22 100
MsaG006891 MtrunA17_Chr8g0386271 48.864 88 44 1 79 165 85 172 5.25e-22 99.0
MsaG006891 MtrunA17_Chr7g0231911 54.217 83 38 0 88 170 145 227 6.28e-22 97.4
MsaG006891 MtrunA17_Chr7g0224821 45.977 87 47 0 79 165 64 150 2.29e-21 96.7
MsaG006891 MtrunA17_Chr2g0308321 47.253 91 47 1 79 168 157 247 2.43e-21 98.6
MsaG006891 MtrunA17_Chr4g0061651 47.872 94 46 2 79 171 112 203 2.64e-21 97.8
MsaG006891 MtrunA17_Chr8g0360361 40.385 104 62 0 92 195 65 168 1.33e-19 87.0
MsaG006891 MtrunA17_Chr2g0282521 43.956 91 51 0 79 169 51 141 2.12e-19 85.5
MsaG006891 MtrunA17_Chr8g0377401 52.055 73 35 0 92 164 162 234 3.63e-19 87.8
MsaG006891 MtrunA17_Chr8g0377421 40.833 120 60 2 63 171 95 214 3.97e-19 89.0
MsaG006891 MtrunA17_Chr3g0132091 43.478 92 52 0 74 165 55 146 8.31e-19 89.0
MsaG006891 MtrunA17_Chr8g0377431 45.070 71 39 0 77 147 136 206 6.94e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006891 AT5G18830.1 46.173 784 365 15 2 741 24 794 0.0 660
MsaG006891 AT5G18830.3 46.173 784 365 15 2 741 24 794 0.0 659
MsaG006891 AT5G18830.2 45.725 772 360 12 2 741 24 768 0.0 654
MsaG006891 AT1G53160.3 49.020 102 47 2 77 173 34 135 6.16e-25 102
MsaG006891 AT1G76580.1 28.629 248 162 4 261 501 482 721 7.25e-25 111
MsaG006891 AT1G76580.1 40.952 105 59 1 91 195 113 214 4.34e-21 99.4
MsaG006891 AT2G33810.1 47.826 92 48 0 78 169 40 131 2.16e-24 99.8
MsaG006891 AT1G02065.1 51.613 93 44 1 84 176 180 271 6.81e-24 103
MsaG006891 AT1G69170.1 48.421 95 49 0 74 168 106 200 1.88e-23 103
MsaG006891 AT2G47070.1 50.000 96 41 2 71 165 90 179 7.07e-23 105
MsaG006891 AT2G47070.1 27.385 325 190 10 273 583 360 652 4.51e-22 102
MsaG006891 AT1G27370.6 46.392 97 52 0 69 165 153 249 9.50e-23 100
MsaG006891 AT1G27360.2 53.333 75 35 0 91 165 174 248 9.75e-23 101
MsaG006891 AT1G27360.1 53.333 75 35 0 91 165 174 248 9.75e-23 101
MsaG006891 AT1G27360.3 53.333 75 35 0 91 165 174 248 9.75e-23 101
MsaG006891 AT1G27360.4 53.333 75 35 0 91 165 174 248 9.75e-23 101
MsaG006891 AT1G27370.1 46.392 97 52 0 69 165 153 249 1.05e-22 101
MsaG006891 AT1G27370.5 46.392 97 52 0 69 165 153 249 1.05e-22 101
MsaG006891 AT1G27370.3 46.392 97 52 0 69 165 153 249 1.05e-22 101
MsaG006891 AT1G27370.2 46.392 97 52 0 69 165 153 249 1.05e-22 101
MsaG006891 AT1G27370.4 46.392 97 52 0 69 165 153 249 1.05e-22 101
MsaG006891 AT1G27370.7 46.392 97 52 0 69 165 153 249 1.05e-22 101
MsaG006891 AT3G57920.1 45.714 105 55 2 70 172 35 139 1.35e-22 100
MsaG006891 AT1G53160.2 45.000 120 56 3 77 186 34 153 2.10e-22 95.5
MsaG006891 AT1G53160.1 45.000 120 56 3 77 186 34 153 2.10e-22 95.5
MsaG006891 AT3G60030.1 54.430 79 36 0 92 170 127 205 5.85e-22 102
MsaG006891 AT3G60030.1 25.305 328 201 11 270 583 398 695 2.54e-19 93.6
MsaG006891 AT3G15270.1 48.352 91 47 0 80 170 51 141 5.99e-22 94.4
MsaG006891 AT2G47070.2 27.385 325 190 10 273 583 231 523 7.29e-22 101
MsaG006891 AT2G47070.2 54.167 48 22 0 118 165 3 50 1.44e-11 68.6
MsaG006891 AT5G43270.3 53.247 77 36 0 89 165 166 242 1.73e-21 98.2
MsaG006891 AT5G43270.1 53.247 77 36 0 89 165 166 242 1.73e-21 98.2
MsaG006891 AT5G43270.2 53.247 77 36 0 89 165 166 242 1.73e-21 98.2
MsaG006891 AT1G20980.1 42.017 119 65 1 76 190 104 222 2.11e-21 100
MsaG006891 AT1G20980.1 24.779 339 225 8 261 587 485 805 4.23e-21 99.4
MsaG006891 AT1G76580.2 40.952 105 59 1 91 195 113 214 2.56e-21 99.8
MsaG006891 AT1G76580.2 30.682 176 114 2 261 429 482 656 3.96e-21 99.0
MsaG006891 AT1G76580.3 40.952 105 59 1 91 195 113 214 2.75e-21 99.8
MsaG006891 AT1G76580.3 30.682 176 114 2 261 429 482 656 5.67e-21 98.6
MsaG006891 AT5G50570.1 50.000 74 37 0 92 165 101 174 5.67e-20 92.8
MsaG006891 AT5G50570.2 50.000 74 37 0 92 165 101 174 5.67e-20 92.8
MsaG006891 AT5G50670.1 50.000 74 37 0 92 165 101 174 5.67e-20 92.8
MsaG006891 AT5G50670.2 50.000 74 37 0 92 165 101 174 5.67e-20 92.8
MsaG006891 AT2G42200.1 52.000 75 36 0 91 165 73 147 8.56e-19 89.7
MsaG006891 AT1G02065.2 48.214 56 29 0 84 139 180 235 1.21e-11 65.9

Find 167 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGATTCAACTATCATTT+TGG 0.092702 2:+11169288 MsaT006891.1:CDS
TCTTCCAGATAGCATCTTTC+TGG 0.114884 2:-11165871 None:intergenic
AACTTTATTCCTGTACTTAT+TGG 0.134941 2:+11169007 MsaT006891.1:CDS
ACATTTATCCACATAATATT+TGG 0.170789 2:-11165421 None:intergenic
CGCTGTCACCGTTAATATTC+TGG 0.195200 2:-11164921 None:intergenic
GTTGATATTTCCGAGCTTAA+AGG 0.202717 2:+11164025 MsaT006891.1:CDS
TTTGTTATGCTTATCAAATT+TGG 0.209664 2:-11169322 None:intergenic
ACTTTATTCCTGTACTTATT+GGG 0.226415 2:+11169008 MsaT006891.1:CDS
GAAGAAGAACCGGTTGTTAA+TGG 0.267583 2:+11164880 MsaT006891.1:CDS
ACTTGGAGCCGCAGCCTTCT+TGG 0.274103 2:-11165209 None:intergenic
AAAGGATATTATAATGAATA+TGG 0.277893 2:+11170231 MsaT006891.1:CDS
CTCTCCTTTCTAAACAATTT+CGG 0.289124 2:+11169083 MsaT006891.1:CDS
CAACTGATAACTTGGCGTTT+TGG 0.290551 2:-11169489 None:intergenic
AGGTTTGAAGCCTTCCATTT+TGG 0.297680 2:-11169452 None:intergenic
GGCGGCATCGGCGCGTTGTC+AGG 0.305792 2:+11163988 MsaT006891.1:CDS
CATGTCAACATCACTTATAT+TGG 0.320229 2:-11169356 None:intergenic
TTAACCTCGGGTCACGCTTT+CGG 0.322042 2:-11163856 None:intergenic
GCTGAAACACAAGCGAATAT+TGG 0.331955 2:+11164964 MsaT006891.1:CDS
ACGGAGTTGTAGAAGGAAAT+TGG 0.333246 2:+11164275 MsaT006891.1:CDS
TCGGCGCGTTGTCAGGTTCC+TGG 0.339982 2:+11163995 MsaT006891.1:CDS
GCTGCTACTGTTGTTCTTGA+TGG 0.340959 2:+11164088 MsaT006891.1:CDS
CCGAAGATCTCTCATCCGTT+TGG 0.354807 2:+11163741 MsaT006891.1:CDS
TAAAAGAAACCCGGCCTGTT+TGG 0.355695 2:-11165157 None:intergenic
CTGTAGGTTGAGAATGAGTC+TGG 0.356081 2:+11168977 MsaT006891.1:intron
AACTGCACGCGCGGCGGCAT+CGG 0.357274 2:+11163976 MsaT006891.1:CDS
TCAAAGATATTGCTATTTGT+TGG 0.367649 2:+11169252 MsaT006891.1:CDS
TCCTAGTTCTCCGTCACAAT+TGG 0.377414 2:-11164388 None:intergenic
TCTAACAAAGCATCAAGTTC+GGG 0.378400 2:-11163920 None:intergenic
TAAGAGTGATTATTCGTCTA+TGG 0.379736 2:+11165032 MsaT006891.1:CDS
ACTCTGCATCTGCTCTAAAC+TGG 0.380253 2:-11170150 None:intergenic
GTTGAACAAACACCTTCTAA+TGG 0.384218 2:-11170404 None:intergenic
TTTCGTCTGGATTAGGGTTA+GGG 0.392019 2:-11163823 None:intergenic
CGAAGATCTCTCATCCGTTT+GGG 0.394668 2:+11163742 MsaT006891.1:CDS
ACTTGCTGCGGCCCAAGTTT+CGG 0.398259 2:+11166247 MsaT006891.1:CDS
AAACAATTTCGGTCTGCATC+TGG 0.400091 2:+11169094 MsaT006891.1:CDS
GTTCGTGCTCGTTTCTTCCC+TGG 0.407134 2:-11163956 None:intergenic
CCAGAGACCCAGAATATTAA+CGG 0.418853 2:+11164913 MsaT006891.1:CDS
TTCTAACAAAGCATCAAGTT+CGG 0.418865 2:-11163921 None:intergenic
TTTGGAAGAGGAGGGAGTTG+TGG 0.423312 2:-11163800 None:intergenic
GAAAATTACATACCGAAACT+TGG 0.426287 2:-11166259 None:intergenic
CAAACGGATGAGAGATCTTC+GGG 0.427353 2:-11163740 None:intergenic
CTTGCAGTGCCAAACAGGCC+GGG 0.431911 2:+11165148 MsaT006891.1:intron
CTTCCAGATAGCATCTTTCT+GGG 0.432338 2:-11165870 None:intergenic
GGGTTAGGGTTTGGAAGAGG+AGG 0.433357 2:-11163809 None:intergenic
ACCGGTTCTTCTTCATGATC+CGG 0.435427 2:-11164871 None:intergenic
GCTCCCAGAAAGATGCTATC+TGG 0.435998 2:+11165867 MsaT006891.1:CDS
TCTGGATTAGGGTTAGGGTT+TGG 0.440583 2:-11163818 None:intergenic
ATCTTCAACAACTGATAACT+TGG 0.440620 2:-11169497 None:intergenic
AAGGGAAACGGAGTTGTAGA+AGG 0.440946 2:+11164268 MsaT006891.1:CDS
GCTTGCAGTGCCAAACAGGC+CGG 0.446028 2:+11165147 MsaT006891.1:intron
CAATATGAAGGCGACTTAGA+AGG 0.446383 2:-11165003 None:intergenic
TTTAGAGCAGATGCAGAGTT+GGG 0.447298 2:+11170154 MsaT006891.1:CDS
CCGTTAATATTCTGGGTCTC+TGG 0.448445 2:-11164913 None:intergenic
TTGCAATGCCAAATATTATG+TGG 0.451793 2:+11165413 MsaT006891.1:CDS
TTAGGGTTAGGGTTTGGAAG+AGG 0.455409 2:-11163812 None:intergenic
GATGCTTTGTTAGAAGATAA+CGG 0.463452 2:+11163929 MsaT006891.1:CDS
GCCGGATCATGAAGAAGAAC+CGG 0.466001 2:+11164870 MsaT006891.1:CDS
ATATATCCCTCTAGCTCTAC+AGG 0.469152 2:-11165358 None:intergenic
AGTTGGCAGTATCCATTAGA+AGG 0.469741 2:+11170392 MsaT006891.1:CDS
CCAAACGGATGAGAGATCTT+CGG 0.470646 2:-11163741 None:intergenic
GTTTAGAGCAGATGCAGAGT+TGG 0.473139 2:+11170153 MsaT006891.1:CDS
CAGACCGAAATTGTTTAGAA+AGG 0.476881 2:-11169087 None:intergenic
AGATCTTCGGGAGGGTCCAT+TGG 0.479717 2:-11163728 None:intergenic
TTAGAAGATAACGGTTTACC+AGG 0.484755 2:+11163938 MsaT006891.1:CDS
GCTGTCACCGTTAATATTCT+GGG 0.485620 2:-11164920 None:intergenic
ATGCTAGAGATGCTATTTGT+CGG 0.486858 2:+11169404 MsaT006891.1:CDS
TGTGTATCTATCTTTCATCA+TGG 0.489539 2:+11170358 MsaT006891.1:CDS
CCTGGGGCCTGCATTTATTG+AGG 0.489676 2:+11168153 MsaT006891.1:CDS
TCATATTGAGGAAAATCTCC+TGG 0.489818 2:+11168135 MsaT006891.1:CDS
AATTGGAGCGGCATAATACT+AGG 0.491494 2:+11164292 MsaT006891.1:CDS
TGTGACGGAGAACTAGGAAT+AGG 0.492998 2:+11164393 MsaT006891.1:CDS
AGGCTATCATCGACGGCATA+GGG 0.494022 2:+11164045 MsaT006891.1:CDS
GTTGACGAACCATTAACAAC+CGG 0.496044 2:-11164889 None:intergenic
GGTTAGGGTTTGGAAGAGGA+GGG 0.496553 2:-11163808 None:intergenic
AAGGCTATCATCGACGGCAT+AGG 0.500460 2:+11164044 MsaT006891.1:CDS
ATCACTTGTTGTTTGTCTTG+TGG 0.502076 2:+11170333 MsaT006891.1:CDS
CTTTATTCCTGTACTTATTG+GGG 0.502295 2:+11169009 MsaT006891.1:CDS
CATCCCACATGCTTTGAAGC+AGG 0.502812 2:+11166192 MsaT006891.1:CDS
TTTGCAGATATTCCAATGGT+TGG 0.503021 2:+11165327 MsaT006891.1:intron
TCTTTATCCCCAATAAGTAC+AGG 0.504320 2:-11169016 None:intergenic
CAGGCATACTTGCCAACCAT+TGG 0.506762 2:-11165339 None:intergenic
TATCTATCTTTCATCATGGG+AGG 0.507164 2:+11170362 MsaT006891.1:CDS
TTGTCTTCAGCCGAGTTATG+TGG 0.515481 2:-11168067 None:intergenic
TCATGGGAGGGTTAACGAGT+TGG 0.515866 2:+11170375 MsaT006891.1:CDS
TACACATGCCCTTCAGCAGT+TGG 0.521989 2:-11169381 None:intergenic
AGTTGTTGAAGATAACAGTC+AGG 0.525980 2:+11169507 MsaT006891.1:CDS
TCCAATTGTGACGGAGAACT+AGG 0.526978 2:+11164387 MsaT006891.1:CDS
AGCCTTCTTGGAAATTCCGC+AGG 0.535663 2:-11165197 None:intergenic
CTAGAGGGATATATCCGCCC+TGG 0.540836 2:+11165367 MsaT006891.1:CDS
TAATACTAGGAGGAGGAGAA+AGG 0.540952 2:+11164305 MsaT006891.1:CDS
ATGATCTTCCGTGTAATGAA+AGG 0.541427 2:+11165921 MsaT006891.1:CDS
AGATCACGCACATAATACAT+AGG 0.541842 2:-11165840 None:intergenic
GACGGCATAGGGTTTGTTTG+CGG 0.543698 2:+11164056 MsaT006891.1:CDS
GTGTATCTATCTTTCATCAT+GGG 0.544893 2:+11170359 MsaT006891.1:CDS
ACGAGCACGAACTGCACGCG+CGG 0.548901 2:+11163967 MsaT006891.1:CDS
ATAACTACCTCAATAAATGC+AGG 0.551106 2:-11168160 None:intergenic
GTGACCGTCAAAATCTTACC+AGG 0.551898 2:+11170272 MsaT006891.1:CDS
CATATTGAGGAAAATCTCCT+GGG 0.554987 2:+11168136 MsaT006891.1:CDS
CTCTTATTGTCACAATATGA+AGG 0.557692 2:-11165015 None:intergenic
CCTCAATAAATGCAGGCCCC+AGG 0.559939 2:-11168153 None:intergenic
AAAAGTCGAGGCAAACATGC+AGG 0.562694 2:+11166149 MsaT006891.1:CDS
TGAAGGCGACTTAGAAGGAT+CGG 0.563449 2:-11164998 None:intergenic
CAATATAAGTGATGTTGACA+TGG 0.564749 2:+11169357 MsaT006891.1:CDS
GATATGTGTCCCTCATATTG+AGG 0.564934 2:+11168123 MsaT006891.1:CDS
AAAATTACATACCGAAACTT+GGG 0.567163 2:-11166258 None:intergenic
AGAAAGATGCTATCTGGAAG+AGG 0.569804 2:+11165873 MsaT006891.1:CDS
AAGGCTGTAGATTCTGCAGT+AGG 0.571229 2:+11164324 MsaT006891.1:CDS
GCTATTTGTCGGAAAGGTGG+AGG 0.572191 2:+11169415 MsaT006891.1:CDS
TTTCCTGCTTCAAAGCATGT+GGG 0.574026 2:-11166195 None:intergenic
TACTTGACCCGCGAGAAAGT+TGG 0.574402 2:-11163885 None:intergenic
AGAGATGCTATTTGTCGGAA+AGG 0.576533 2:+11169409 MsaT006891.1:CDS
ATCTATCTTTCATCATGGGA+GGG 0.580834 2:+11170363 MsaT006891.1:CDS
ACTTGCTCCAACTTTCTCGC+GGG 0.581441 2:+11163878 MsaT006891.1:CDS
GAATATCGTCGTCGAAGTCG+AGG 0.582065 2:-11163778 None:intergenic
CGGACTTCGATGAAGGGAAA+CGG 0.583712 2:+11164256 MsaT006891.1:CDS
TGTTTCAGCAGAAGCAACAA+AGG 0.585054 2:-11164950 None:intergenic
CGTTCCGTCTCCACATAACT+CGG 0.585530 2:+11168057 MsaT006891.1:CDS
AGTTGGAGCAAGTTAACCTC+GGG 0.590174 2:-11163868 None:intergenic
AGCTTAAAGGCTATCATCGA+CGG 0.590404 2:+11164038 MsaT006891.1:CDS
ACTTGCTTGTAGATATAGCA+TGG 0.593375 2:+11169173 MsaT006891.1:CDS
AACTTGCTCCAACTTTCTCG+CGG 0.593749 2:+11163877 MsaT006891.1:CDS
AGCACGAACTGCACGCGCGG+CGG 0.596963 2:+11163970 MsaT006891.1:CDS
CGGAATTTCCAAGAAGGCTG+CGG 0.598319 2:+11165201 MsaT006891.1:CDS
GATGCTATTTGTCGGAAAGG+TGG 0.601141 2:+11169412 MsaT006891.1:CDS
AAGTATGCCTGTAGAGCTAG+AGG 0.601194 2:+11165351 MsaT006891.1:CDS
CGTAAAGCTTAAAAGAAACC+CGG 0.601561 2:-11165166 None:intergenic
GATCACGCACATAATACATA+GGG 0.602014 2:-11165839 None:intergenic
CGAAGTCGAGGAGGTAAGAG+AGG 0.603612 2:-11163766 None:intergenic
GAGGTAAGAGAGGTCCCAAA+CGG 0.604613 2:-11163756 None:intergenic
TGACATGGACCAACTGCTGA+AGG 0.605178 2:+11169372 MsaT006891.1:CDS
AATGATGATTCCAATTGTGA+CGG 0.605762 2:+11164378 MsaT006891.1:CDS
TCAAGTTCGGGGCATGCACA+TGG 0.606489 2:-11163908 None:intergenic
AGAGATACTGTCAACAGTGT+GGG 0.614903 2:+11164119 MsaT006891.1:CDS
TCGAGATCTCAAGAATCCCC+TGG 0.617392 2:-11170290 None:intergenic
CTAATCCAGACGAAAACGAT+AGG 0.618355 2:+11163831 MsaT006891.1:CDS
CTTTGAAGCAGGAAAACCCA+TGG 0.618843 2:+11166203 MsaT006891.1:CDS
TAGAGGGATATATCCGCCCT+GGG 0.627129 2:+11165368 MsaT006891.1:CDS
AGCGGCATAATACTAGGAGG+AGG 0.628758 2:+11164298 MsaT006891.1:CDS
ATCGTCAGGATCGTACACCC+AGG 0.629008 2:-11165385 None:intergenic
AGGCAAACATGCAGGCACCT+AGG 0.631524 2:+11166157 MsaT006891.1:CDS
CGGATGAGAGATCTTCGGGA+GGG 0.639323 2:-11163736 None:intergenic
AAGTTGGAGCAAGTTAACCT+CGG 0.643009 2:-11163869 None:intergenic
GGATGAATATAGTGAAGCCT+AGG 0.643635 2:-11166174 None:intergenic
ATCCTGCGGAATTTCCAAGA+AGG 0.645595 2:+11165195 MsaT006891.1:CDS
TCAGCCGAGTTATGTGGAGA+CGG 0.653109 2:-11168061 None:intergenic
TCGATGATAGCCTTTAAGCT+CGG 0.653435 2:-11164035 None:intergenic
TCGTCAGGATCGTACACCCA+GGG 0.658661 2:-11165384 None:intergenic
GCTTTACGACTGGAATCCTG+CGG 0.658919 2:+11165181 MsaT006891.1:CDS
ATCATTCAGATGAATTAGAG+CGG 0.660105 2:-11165898 None:intergenic
CTTGTGGAAGTAACTTGCTG+CGG 0.661577 2:+11166235 MsaT006891.1:CDS
GGATCCGAAAGCGTGACCCG+AGG 0.668770 2:+11163852 MsaT006891.1:CDS
GAAATATCAACTTCACAACC+AGG 0.673466 2:-11164013 None:intergenic
AATTTCTGTGACAAAAGTCG+AGG 0.673573 2:+11166137 MsaT006891.1:CDS
GAAACAAACCTTTCATTACA+CGG 0.677907 2:-11165929 None:intergenic
TATCGTCGTCGAAGTCGAGG+AGG 0.681010 2:-11163775 None:intergenic
CTAACAAAGCATCAAGTTCG+GGG 0.681130 2:-11163919 None:intergenic
AAGAGATACTGTCAACAGTG+TGG 0.681163 2:+11164118 MsaT006891.1:CDS
GACATGGACCAACTGCTGAA+GGG 0.681962 2:+11169373 MsaT006891.1:CDS
GTTGTAGAAGGAAATTGGAG+CGG 0.686927 2:+11164280 MsaT006891.1:CDS
ACGGATGAGAGATCTTCGGG+AGG 0.691526 2:-11163737 None:intergenic
TGACCGTCAAAATCTTACCA+GGG 0.692658 2:+11170273 MsaT006891.1:CDS
TAGAAGATAACGGTTTACCA+GGG 0.694372 2:+11163939 MsaT006891.1:CDS
GACCGTCAAAATCTTACCAG+GGG 0.695671 2:+11170274 MsaT006891.1:CDS
TGGAGCGGCATAATACTAGG+AGG 0.705490 2:+11164295 MsaT006891.1:CDS
AGTATGCCTGTAGAGCTAGA+GGG 0.705762 2:+11165352 MsaT006891.1:CDS
ATATTGAGGAAAATCTCCTG+GGG 0.711856 2:+11168137 MsaT006891.1:CDS
TCAGGATCGTACACCCAGGG+CGG 0.738843 2:-11165381 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 11163731 11170432 11163731 ID=MsaG006891
Chr2 mRNA 11163731 11170432 11163731 ID=MsaT006891.1;Parent=MsaG006891
Chr2 exon 11163731 11164143 11163731 ID=MsaT006891.1.exon1;Parent=MsaT006891.1
Chr2 CDS 11163731 11164143 11163731 ID=cds.MsaT006891.1;Parent=MsaT006891.1
Chr2 exon 11164242 11164414 11164242 ID=MsaT006891.1.exon2;Parent=MsaT006891.1
Chr2 CDS 11164242 11164414 11164242 ID=cds.MsaT006891.1;Parent=MsaT006891.1
Chr2 exon 11164827 11165053 11164827 ID=MsaT006891.1.exon3;Parent=MsaT006891.1
Chr2 CDS 11164827 11165053 11164827 ID=cds.MsaT006891.1;Parent=MsaT006891.1
Chr2 exon 11165155 11165229 11165155 ID=MsaT006891.1.exon4;Parent=MsaT006891.1
Chr2 CDS 11165155 11165229 11165155 ID=cds.MsaT006891.1;Parent=MsaT006891.1
Chr2 exon 11165334 11165441 11165334 ID=MsaT006891.1.exon5;Parent=MsaT006891.1
Chr2 CDS 11165334 11165441 11165334 ID=cds.MsaT006891.1;Parent=MsaT006891.1
Chr2 exon 11165828 11165942 11165828 ID=MsaT006891.1.exon6;Parent=MsaT006891.1
Chr2 CDS 11165828 11165942 11165828 ID=cds.MsaT006891.1;Parent=MsaT006891.1
Chr2 exon 11166133 11166268 11166133 ID=MsaT006891.1.exon7;Parent=MsaT006891.1
Chr2 CDS 11166133 11166268 11166133 ID=cds.MsaT006891.1;Parent=MsaT006891.1
Chr2 exon 11168012 11168174 11168012 ID=MsaT006891.1.exon8;Parent=MsaT006891.1
Chr2 CDS 11168012 11168174 11168012 ID=cds.MsaT006891.1;Parent=MsaT006891.1
Chr2 exon 11168983 11169528 11168983 ID=MsaT006891.1.exon9;Parent=MsaT006891.1
Chr2 CDS 11168983 11169528 11168983 ID=cds.MsaT006891.1;Parent=MsaT006891.1
Chr2 exon 11170145 11170432 11170145 ID=MsaT006891.1.exon10;Parent=MsaT006891.1
Chr2 CDS 11170145 11170432 11170145 ID=cds.MsaT006891.1;Parent=MsaT006891.1
Gene Sequence

>MsaT006891.1

ATGGACCCTCCCGAAGATCTCTCATCCGTTTGGGACCTCTCTTACCTCCTCGACTTCGACGACGATATTCCACAACTCCCTCCTCTTCCAAACCCTAACCCTAATCCAGACGAAAACGATAGGATCCGAAAGCGTGACCCGAGGTTAACTTGCTCCAACTTTCTCGCGGGTCAAGTACCATGTGCATGCCCCGAACTTGATGCTTTGTTAGAAGATAACGGTTTACCAGGGAAGAAACGAGCACGAACTGCACGCGCGGCGGCATCGGCGCGTTGTCAGGTTCCTGGTTGTGAAGTTGATATTTCCGAGCTTAAAGGCTATCATCGACGGCATAGGGTTTGTTTGCGGTGTGCAAATGCTGCTACTGTTGTTCTTGATGGTGATGTGAAGAGATACTGTCAACAGTGTGGGAAGTTTCATGTTTTATCGGACTTCGATGAAGGGAAACGGAGTTGTAGAAGGAAATTGGAGCGGCATAATACTAGGAGGAGGAGAAAGGCTGTAGATTCTGCAGTAGGAGTTGATAATGAAGTTCAAACTGTTACTCAAAATGATGATTCCAATTGTGACGGAGAACTAGGAATAGATTATTCGAATTTGAGTAGTGAGAATATCGAAAAAAGAGTGTTGCCGGATCATGAAGAAGAACCGGTTGTTAATGGTTCGTCAACACCAGAGACCCAGAATATTAACGGTGACAGCGTCGTTTCCTTTGTTGCTTCTGCTGAAACACAAGCGAATATTGGAAAAGATGTTTCCGATCCTTCTAAGTCGCCTTCATATTGTGACAATAAGAGTGATTATTCGTCTATGTGCCAAACAGGCCGGGTTTCTTTTAAGCTTTACGACTGGAATCCTGCGGAATTTCCAAGAAGGCTGCGGCTCCAAATATTCCAATGGTTGGCAAGTATGCCTGTAGAGCTAGAGGGATATATCCGCCCTGGGTGTACGATCCTGACGATTTTTATTGCAATGCCAAATATTATGTGGATAAATTTACTCAAAGACCCTATGTATTATGTGCGTGATCTTGTTGCTCCCAGAAAGATGCTATCTGGAAGAGGAACCGCTCTAATTCATCTGAATGATATGATCTTCCGTGTAATGAAAGATGGAATTTCTGTGACAAAAGTCGAGGCAAACATGCAGGCACCTAGGCTTCACTATATTCATCCCACATGCTTTGAAGCAGGAAAACCCATGGAATTTTTCGCTTGTGGAAGTAACTTGCTGCGGCCCAAGTTTCGGCTTCTTGTATCTTTTTATGGAAAGTATCTGAAGTGTGAGTATTGCGTTCCGTCTCCACATAACTCGGCTGAAGACAATATTTCTTGTGCTTTTGATAATCAGTTATATAAGATATGTGTCCCTCATATTGAGGAAAATCTCCTGGGGCCTGCATTTATTGAGGTTGAGAATGAGTCTGGTTTATCAAACTTTATTCCTGTACTTATTGGGGATAAAGAAATTTGCACTGAAATGAAGATATTGCAACAAAAATTAGACGCTTCTCTCCTTTCTAAACAATTTCGGTCTGCATCTGGCAGTTCTATTTGTAGCTCATGTGAAGCTTTTGCACATATTCACACATCATCATCAGACTTGCTTGTAGATATAGCATGGTTACTTAAAGACCCGACTTCAGAAATTTTTGACAGAATGGTATCTGCTTCACAGATTCAAAGATATTGCTATTTGTTGGATTTTCTAATATGCAATGATTCAACTATCATTTTGGGAAAAATATTGCCAAATTTGATAAGCATAACAAAAAGCATGAAATCCAATATAAGTGATGTTGACATGGACCAACTGCTGAAGGGCATGTGTAATGCTAGAGATGCTATTTGTCGGAAAGGTGGAGGTATAGTTTTGAACTCCAAAATGGAAGGCTTCAAACCTGCTCTGTGCAGTTCCCAAAACGCCAAGTTATCAGTTGTTGAAGATAACAGTCAGGGTATCCAGTTTAGAGCAGATGCAGAGTTGGGAGTTCTGAGCAGTCTCGCTTCCGATGAAAAAAATCAGAAGATACCGCTTTTGAAAAAGGATATTATAATGAATATGGAAGAGTTGCCTAAAAGATGTGACCGTCAAAATCTTACCAGGGGATTCTTGAGATCTCGACCAACTATTTTTGTGCTTGTATCACTTGTTGTTTGTCTTGTGGTATGTGTATCTATCTTTCATCATGGGAGGGTTAACGAGTTGGCAGTATCCATTAGAAGGTGTTTGTTCAACCATTGA
Protein sequence

>MsaT006891.1

MDPPEDLSSVWDLSYLLDFDDDIPQLPPLPNPNPNPDENDRIRKRDPRLTCSNFLAGQVPCACPELDALLEDNGLPGKKRARTARAAASARCQVPGCEVDISELKGYHRRHRVCLRCANAATVVLDGDVKRYCQQCGKFHVLSDFDEGKRSCRRKLERHNTRRRRKAVDSAVGVDNEVQTVTQNDDSNCDGELGIDYSNLSSENIEKRVLPDHEEEPVVNGSSTPETQNINGDSVVSFVASAETQANIGKDVSDPSKSPSYCDNKSDYSSMCQTGRVSFKLYDWNPAEFPRRLRLQIFQWLASMPVELEGYIRPGCTILTIFIAMPNIMWINLLKDPMYYVRDLVAPRKMLSGRGTALIHLNDMIFRVMKDGISVTKVEANMQAPRLHYIHPTCFEAGKPMEFFACGSNLLRPKFRLLVSFYGKYLKCEYCVPSPHNSAEDNISCAFDNQLYKICVPHIEENLLGPAFIEVENESGLSNFIPVLIGDKEICTEMKILQQKLDASLLSKQFRSASGSSICSSCEAFAHIHTSSSDLLVDIAWLLKDPTSEIFDRMVSASQIQRYCYLLDFLICNDSTIILGKILPNLISITKSMKSNISDVDMDQLLKGMCNARDAICRKGGGIVLNSKMEGFKPALCSSQNAKLSVVEDNSQGIQFRADAELGVLSSLASDEKNQKIPLLKKDIIMNMEELPKRCDRQNLTRGFLRSRPTIFVLVSLVVCLVVCVSIFHHGRVNELAVSIRRCLFNH*