AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG006928

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006928 MtrunA17_Chr2g0287061 60.586 614 167 11 20 584 174 761 0.0 658
MsaG006928 MtrunA17_Chr2g0287061 97.251 291 5 1 1 288 1 291 0.0 591
MsaG006928 MtrunA17_Chr5g0448511 39.542 306 176 4 283 580 152 456 3.10e-69 233
MsaG006928 MtrunA17_Chr5g0448761 38.062 289 172 4 299 581 398 685 7.71e-60 213
MsaG006928 MtrunA17_Chr5g0448761 38.889 108 49 2 178 268 58 165 5.74e-12 68.9
MsaG006928 MtrunA17_Chr5g0440451 39.350 277 163 4 307 580 387 661 5.86e-54 197
MsaG006928 MtrunA17_Chr5g0440451 37.778 90 56 0 28 117 42 131 3.25e-12 69.7
MsaG006928 MtrunA17_Chr5g0440451 35.870 92 59 0 179 270 42 133 2.83e-11 66.6
MsaG006928 MtrunA17_Chr1g0202341 35.154 293 172 6 300 580 383 669 7.07e-50 183
MsaG006928 MtrunA17_Chr1g0202341 30.488 164 95 4 178 325 49 209 6.62e-13 71.6
MsaG006928 MtrunA17_Chr3g0118611 36.184 304 187 5 283 581 258 559 1.20e-49 184
MsaG006928 MtrunA17_Chr2g0324011 25.347 576 355 19 24 530 5 574 3.05e-43 163
MsaG006928 MtrunA17_Chr3g0080351 25.284 617 387 17 24 578 71 675 9.55e-43 164
MsaG006928 MtrunA17_Chr2g0291581 32.248 307 196 6 282 576 369 675 9.27e-42 160
MsaG006928 MtrunA17_Chr2g0291581 32.759 116 68 2 11 117 54 168 3.96e-11 65.9
MsaG006928 MtrunA17_Chr2g0329031 33.684 285 181 5 298 579 335 614 4.17e-39 152
MsaG006928 MtrunA17_Chr1g0207551 35.371 229 143 2 299 524 363 589 5.64e-39 152
MsaG006928 MtrunA17_Chr5g0441581 33.778 225 142 2 305 522 357 581 6.59e-33 134
MsaG006928 MtrunA17_Chr2g0296781 34.498 229 130 6 308 522 332 554 5.17e-30 125
MsaG006928 MtrunA17_Chr6g0458391 31.271 291 142 8 5 268 67 326 6.28e-30 125
MsaG006928 MtrunA17_Chr6g0458391 30.000 230 156 2 297 523 537 764 3.66e-28 119
MsaG006928 MtrunA17_Chr6g0458391 29.480 173 96 4 119 268 22 191 7.55e-16 81.3
MsaG006928 MtrunA17_Chr6g0477261 33.186 226 137 5 308 522 221 443 2.21e-29 123
MsaG006928 MtrunA17_Chr1g0196541 32.301 226 143 5 305 521 329 553 8.91e-29 121
MsaG006928 MtrunA17_Chr8g0363841 31.780 236 151 5 300 528 349 581 1.51e-28 120
MsaG006928 MtrunA17_Chr1g0203721 27.208 283 198 3 299 575 397 677 2.04e-27 117
MsaG006928 MtrunA17_Chr1g0203721 34.343 99 52 1 182 267 65 163 3.43e-12 69.7
MsaG006928 MtrunA17_Chr1g0203721 38.776 98 47 1 31 115 65 162 1.00e-11 68.2
MsaG006928 MtrunA17_Chr2g0294841 23.569 594 316 22 29 519 45 603 7.34e-26 112
MsaG006928 MtrunA17_Chr1g0203051 28.241 216 150 3 300 511 407 621 9.51e-25 108
MsaG006928 MtrunA17_Chr2g0308841 27.232 224 156 4 305 523 5 226 2.21e-23 100
MsaG006928 MtrunA17_Chr3g0082121 25.694 288 197 7 300 578 368 647 1.82e-22 102
MsaG006928 MtrunA17_Chr2g0329421 42.157 102 47 1 179 268 26 127 7.49e-22 94.0
MsaG006928 MtrunA17_Chr2g0329421 41.176 102 48 1 28 117 26 127 2.91e-18 84.0
MsaG006928 MtrunA17_Chr4g0033611 42.202 109 51 1 179 275 75 183 1.01e-21 95.1
MsaG006928 MtrunA17_Chr4g0033611 33.333 150 70 3 28 150 75 221 2.21e-16 79.3
MsaG006928 MtrunA17_Chr2g0276841 27.189 217 149 3 308 521 416 626 3.99e-21 97.8
MsaG006928 MtrunA17_Chr2g0287651 26.667 240 149 5 300 524 405 632 1.56e-20 96.3
MsaG006928 MtrunA17_Chr2g0290391 27.419 248 164 7 279 521 374 610 1.35e-19 92.8
MsaG006928 MtrunA17_Chr2g0291591 34.667 150 92 2 299 442 390 539 1.52e-19 92.4
MsaG006928 MtrunA17_Chr2g0291591 29.091 165 99 4 123 278 16 171 1.07e-11 67.8
MsaG006928 MtrunA17_Chr2g0329361 36.735 147 67 3 26 152 58 198 2.51e-19 87.8
MsaG006928 MtrunA17_Chr2g0329361 33.019 106 56 1 178 268 59 164 1.15e-13 71.2
MsaG006928 MtrunA17_Chr7g0233911 40.541 111 52 1 28 124 33 143 3.94e-19 86.3
MsaG006928 MtrunA17_Chr7g0233911 44.231 104 44 2 179 268 33 136 5.10e-18 83.2
MsaG006928 MtrunA17_Chr3g0078041 39.423 104 49 1 28 117 49 152 3.34e-18 84.0
MsaG006928 MtrunA17_Chr3g0078041 41.346 104 47 1 179 268 49 152 3.92e-17 80.9
MsaG006928 MtrunA17_Chr1g0198761 27.442 215 148 3 308 519 398 607 9.44e-18 87.0
MsaG006928 MtrunA17_Chr7g0258861 36.538 104 51 2 179 268 9 111 1.54e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG006928 AT5G18960.2 64.000 300 107 1 285 584 490 788 1.19e-128 396
MsaG006928 AT5G18960.2 45.845 349 161 9 1 328 14 355 6.09e-83 276
MsaG006928 AT5G18960.1 64.000 300 107 1 285 584 490 788 1.19e-128 396
MsaG006928 AT5G18960.1 45.845 349 161 9 1 328 14 355 6.09e-83 276
MsaG006928 AT3G06250.2 66.551 287 95 1 298 584 479 764 2.36e-128 394
MsaG006928 AT3G06250.2 55.914 279 111 5 1 268 1 278 3.40e-95 308
MsaG006928 AT3G06250.2 50.000 112 55 1 158 269 7 117 2.22e-30 127
MsaG006928 AT3G06250.2 56.989 93 40 0 26 118 187 279 3.95e-29 123
MsaG006928 AT3G06250.1 66.551 287 95 1 298 584 479 764 2.36e-128 394
MsaG006928 AT3G06250.1 55.914 279 111 5 1 268 1 278 3.40e-95 308
MsaG006928 AT3G06250.1 50.000 112 55 1 158 269 7 117 2.22e-30 127
MsaG006928 AT3G06250.1 56.989 93 40 0 26 118 187 279 3.95e-29 123
MsaG006928 AT3G06250.3 66.551 287 95 1 298 584 479 764 2.36e-128 394
MsaG006928 AT3G06250.3 55.914 279 111 5 1 268 1 278 3.40e-95 308
MsaG006928 AT3G06250.3 50.000 112 55 1 158 269 7 117 2.22e-30 127
MsaG006928 AT3G06250.3 56.989 93 40 0 26 118 187 279 3.95e-29 123
MsaG006928 AT4G38180.1 29.493 651 349 23 20 580 63 693 1.15e-69 240
MsaG006928 AT2G27110.3 37.209 301 185 3 283 580 191 490 7.01e-56 201
MsaG006928 AT2G27110.2 37.209 301 185 3 283 580 336 635 2.78e-55 201
MsaG006928 AT2G27110.1 37.209 301 185 3 283 580 336 635 2.78e-55 201
MsaG006928 AT4G38170.1 34.539 304 192 4 284 581 155 457 4.05e-55 196
MsaG006928 AT1G52520.1 26.677 626 370 18 29 580 85 695 6.28e-55 198
MsaG006928 AT4G15090.1 33.214 280 180 3 299 575 383 658 7.90e-43 164
MsaG006928 AT4G15090.5 33.214 280 180 3 299 575 383 658 7.90e-43 164
MsaG006928 AT4G15090.3 33.214 280 180 3 299 575 383 658 7.90e-43 164
MsaG006928 AT4G15090.2 33.214 280 180 3 299 575 383 658 7.90e-43 164
MsaG006928 AT4G15090.4 33.214 280 180 3 299 575 383 658 7.90e-43 164
MsaG006928 AT3G22170.2 32.734 278 175 4 298 570 391 661 3.38e-41 160
MsaG006928 AT3G22170.1 32.734 278 175 4 298 570 391 661 3.38e-41 160
MsaG006928 AT1G76320.3 35.745 235 144 3 294 525 315 545 5.21e-37 146
MsaG006928 AT1G76320.2 36.364 231 140 3 298 525 319 545 5.65e-37 147
MsaG006928 AT1G76320.1 36.364 231 140 3 298 525 319 545 6.55e-37 146
MsaG006928 AT1G76320.4 36.364 231 140 3 298 525 319 545 6.55e-37 146
MsaG006928 AT1G80010.1 27.848 316 219 5 274 580 402 717 6.40e-32 131
MsaG006928 AT1G80010.1 32.143 112 67 1 16 118 82 193 1.55e-11 67.8
MsaG006928 AT2G32250.8 32.906 234 149 3 298 525 285 516 8.41e-32 131
MsaG006928 AT2G32250.7 32.906 234 149 3 298 525 285 516 8.92e-32 131
MsaG006928 AT2G32250.2 32.906 234 149 3 298 525 326 557 9.21e-32 131
MsaG006928 AT2G32250.4 32.906 234 149 3 298 525 326 557 9.21e-32 131
MsaG006928 AT2G32250.6 32.906 234 149 3 298 525 326 557 9.50e-32 131
MsaG006928 AT2G32250.1 32.906 234 149 3 298 525 326 557 9.50e-32 131
MsaG006928 AT2G32250.3 32.906 234 149 3 298 525 326 557 9.50e-32 131
MsaG006928 AT2G32250.5 32.906 234 149 3 298 525 326 557 1.10e-31 131
MsaG006928 AT1G10240.2 24.870 575 332 24 29 521 48 604 1.51e-30 127
MsaG006928 AT1G10240.1 24.870 575 332 24 29 521 48 604 1.51e-30 127
MsaG006928 AT4G19990.6 30.677 251 165 3 307 549 339 588 4.45e-27 117
MsaG006928 AT4G19990.2 30.677 251 165 3 307 549 339 588 4.45e-27 117
MsaG006928 AT4G19990.3 30.677 251 165 3 307 549 339 588 5.27e-27 116
MsaG006928 AT4G19990.4 30.677 251 165 3 307 549 339 588 5.27e-27 116
MsaG006928 AT4G19990.5 30.677 251 165 3 307 549 339 588 5.70e-27 116
MsaG006928 AT4G19990.1 30.677 251 165 3 307 549 282 531 5.91e-27 116
MsaG006928 AT4G19990.7 30.677 251 165 3 307 549 353 602 6.45e-27 116
MsaG006928 AT5G28530.4 21.887 530 344 16 29 519 55 553 6.01e-24 106
MsaG006928 AT5G28530.5 21.509 530 342 16 29 519 55 549 8.86e-24 106
MsaG006928 AT3G59470.3 48.039 102 41 1 179 268 69 170 1.02e-23 100
MsaG006928 AT3G59470.3 34.161 161 73 3 14 148 62 215 3.53e-19 87.8
MsaG006928 AT3G59470.1 48.039 102 41 1 179 268 69 170 1.02e-23 100
MsaG006928 AT3G59470.1 34.161 161 73 3 14 148 62 215 3.53e-19 87.8
MsaG006928 AT3G59470.2 48.039 102 41 1 179 268 81 182 1.34e-23 100
MsaG006928 AT3G59470.2 34.161 161 73 3 14 148 74 227 4.88e-19 87.8
MsaG006928 AT3G07500.1 38.532 109 54 1 173 268 26 134 2.29e-19 87.4
MsaG006928 AT3G07500.1 39.806 103 49 1 28 117 32 134 2.56e-16 78.6
MsaG006928 AT4G12850.4 42.982 114 52 2 179 279 10 123 1.72e-18 82.8
MsaG006928 AT4G12850.4 42.574 101 48 1 28 118 10 110 2.05e-18 82.4
MsaG006928 AT4G12850.1 42.574 101 48 1 28 118 10 110 2.72e-18 83.6
MsaG006928 AT4G12850.1 40.984 122 52 2 179 280 10 131 3.85e-18 83.2
MsaG006928 AT4G12850.2 40.909 110 52 2 19 118 4 110 3.53e-18 81.6
MsaG006928 AT4G12850.2 44.000 100 46 1 179 268 10 109 1.63e-17 79.7
MsaG006928 AT2G43280.1 39.815 108 51 1 24 117 17 124 4.24e-18 83.6
MsaG006928 AT2G43280.1 40.952 105 48 2 178 268 20 124 1.13e-14 73.6
MsaG006928 AT5G28530.1 25.283 265 178 6 267 519 360 616 8.23e-18 87.8
MsaG006928 AT5G28530.3 24.528 265 176 6 267 519 360 612 1.19e-17 87.0
MsaG006928 AT5G28530.2 24.528 265 176 6 267 519 360 612 1.19e-17 87.0
MsaG006928 AT2G43280.2 33.835 133 74 1 44 162 2 134 1.81e-14 72.0
MsaG006928 AT2G43280.2 40.909 88 38 2 195 268 2 89 7.65e-12 64.7

Find 118 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATGCTCATGCAGCATATT+TGG 0.062384 2:+11559879 MsaT006928.1:CDS
CTGGGTTCCGTTCTATTTAA+AGG 0.153193 2:+11560848 MsaT006928.1:CDS
TTCTTTGGTGGAAGTTTAAA+TGG 0.183946 2:+11560915 MsaT006928.1:CDS
AGGGTTCAGTTAAATGATTC+TGG 0.241282 2:+11559574 MsaT006928.1:CDS
CATGCAGCATATTTGGGTTT+TGG 0.247228 2:+11559886 MsaT006928.1:CDS
TTGCAGTGCTTTAGTTATCT+TGG 0.278930 2:+11561125 MsaT006928.1:CDS
GTAGAAGACTCAATAGCTTT+TGG 0.284582 2:-11559679 None:intergenic
CTTTATCGATCGAGAACTAA+TGG 0.295969 2:+11559472 MsaT006928.1:CDS
GATTCCATGCTTTCTTTCAT+AGG 0.296754 2:-11560891 None:intergenic
ATAACACCAAAGGGAGAAAT+AGG 0.311248 2:-11559754 None:intergenic
GAAAGCATGGAATCATTCTT+TGG 0.319532 2:+11560900 MsaT006928.1:CDS
GGTGAACCTATTTCTCCCTT+TGG 0.320761 2:+11559748 MsaT006928.1:CDS
CATGCTCATGCAGCATATTT+GGG 0.329135 2:+11559880 MsaT006928.1:CDS
TAAAATTGGTAGGCTTCATT+TGG 0.352504 2:-11559856 None:intergenic
TGGGTTCCGTTCTATTTAAA+GGG 0.358637 2:+11560849 MsaT006928.1:CDS
CTGCATGAGCATGGTAAAAT+TGG 0.364754 2:-11559870 None:intergenic
ATCGAATGGGATTTAAAGTT+CGG 0.379216 2:+11559440 MsaT006928.1:CDS
AAAAGATCATAATCATGATC+TGG 0.383971 2:+11560074 MsaT006928.1:CDS
CTGGTTGTCCAGCATTCATA+AGG 0.391197 2:+11559554 MsaT006928.1:CDS
AGTGCTGTACTCGTCAAATA+TGG 0.393973 2:+11560777 MsaT006928.1:CDS
CGCTTTGTGTGCTCAAAAGA+AGG 0.394545 2:+11559964 MsaT006928.1:CDS
AGGGTCCCTCAGCAGATATT+TGG 0.395130 2:+11561166 MsaT006928.1:CDS
TCGCCGGTCTGATGGGTAAT+GGG 0.413317 2:+11560688 MsaT006928.1:CDS
GCCTTACAGCCAACTCTTGA+AGG 0.417533 2:-11559997 None:intergenic
CTTCTGCACTGTTCTTCTAC+TGG 0.426641 2:-11561059 None:intergenic
TGGTAATGAGTAATGCAATT+GGG 0.428684 2:+11559326 MsaT006928.1:CDS
CCCTTCAAGAGTTGGCTGTA+AGG 0.428884 2:+11559996 MsaT006928.1:CDS
TGTCAAATTCATCGACTGTC+TGG 0.437658 2:-11560746 None:intergenic
CTCGCCGGTCTGATGGGTAA+TGG 0.438659 2:+11560687 MsaT006928.1:CDS
AACTGAACCCTTATGAATGC+TGG 0.443026 2:-11559562 None:intergenic
TTCATTCCGCGTTATGAAAG+AGG 0.443774 2:+11560957 MsaT006928.1:CDS
GCTACAACTAATGTCAAGAT+TGG 0.453950 2:-11561243 None:intergenic
CTGGTAATGAGTAATGCAAT+TGG 0.460733 2:+11559325 MsaT006928.1:CDS
TCATGATCTGGGCCTTGAAA+AGG 0.461115 2:+11560086 MsaT006928.1:CDS
ATATCTTATGGTAATCGAAT+GGG 0.462953 2:+11559427 MsaT006928.1:CDS
TTCTTTGTAGACACGTGTTG+AGG 0.466046 2:+11561291 MsaT006928.1:CDS
TAAGTGTGGAAATGATGATA+TGG 0.473912 2:+11561193 MsaT006928.1:CDS
AAAAGAAGAACTTGAAGTAC+AGG 0.474189 2:+11559795 MsaT006928.1:CDS
AGGATCATGGAGTAGCTCTC+AGG 0.475535 2:+11561406 MsaT006928.1:CDS
GGATTTAAAATCTATGAAGA+AGG 0.480432 2:+11561146 MsaT006928.1:CDS
GAGTAATGCAATTGGGGAGG+AGG 0.480640 2:+11559333 MsaT006928.1:CDS
GCATCCTGTTGGTACTGAAC+TGG 0.481228 2:+11559306 MsaT006928.1:CDS
TTTATCGATCGAGAACTAAT+GGG 0.481588 2:+11559473 MsaT006928.1:CDS
AGCCAACTCTTGAAGGGTGC+TGG 0.486059 2:-11559990 None:intergenic
AGGATTAAGAATCAACCATC+AGG 0.487093 2:+11560027 MsaT006928.1:CDS
ACCCTCTCGCAAAATCTCAT+AGG 0.490493 2:-11561519 None:intergenic
ACCAACATATGGTTCGATCC+TGG 0.490539 2:-11559818 None:intergenic
CTGAACTCTTGACCAACATA+TGG 0.491150 2:-11559829 None:intergenic
GTTAAATGATTCTGGAAAGT+GGG 0.491161 2:+11559582 MsaT006928.1:CDS
ATTCATCGACTGTCTGGCTC+TGG 0.491387 2:-11560740 None:intergenic
CATCAGCTCAGTTCAAGAAC+TGG 0.495226 2:+11559535 MsaT006928.1:CDS
AGGAAGAAAATGCTTGAAGT+AGG 0.495697 2:+11559721 MsaT006928.1:CDS
CAAGAGCTCGCCGGTCTGAT+GGG 0.495940 2:+11560681 MsaT006928.1:CDS
TGAACTCTGTGAGAGGTGTT+TGG 0.496070 2:-11560938 None:intergenic
AGAATCAACCATCAGGAAAG+TGG 0.496214 2:+11560034 MsaT006928.1:CDS
CAATTCAGTGTTTACCTCCT+CGG 0.500177 2:-11560148 None:intergenic
ATGGATTGAAGGACAATGCT+TGG 0.502036 2:+11560796 MsaT006928.1:CDS
AAAGATCATAATCATGATCT+GGG 0.502626 2:+11560075 MsaT006928.1:CDS
TTCCAGCACCCTTCAAGAGT+TGG 0.503277 2:+11559988 MsaT006928.1:CDS
AGTTAAATGATTCTGGAAAG+TGG 0.505016 2:+11559581 MsaT006928.1:CDS
ACTCGTCAAATATGGATTGA+AGG 0.507225 2:+11560785 MsaT006928.1:CDS
CTACTCCATGATCCTATGTC+AGG 0.507285 2:-11561398 None:intergenic
ACAAGAGCTCGCCGGTCTGA+TGG 0.507295 2:+11560680 MsaT006928.1:CDS
TATATCTTATGGTAATCGAA+TGG 0.509718 2:+11559426 MsaT006928.1:CDS
ATTACCAGTTCAGTACCAAC+AGG 0.512740 2:-11559310 None:intergenic
GTATGAGAAGCGTGCATCCT+GGG 0.521503 2:+11560830 MsaT006928.1:CDS
ATGGGATTTAAAGTTCGGAT+TGG 0.522097 2:+11559445 MsaT006928.1:CDS
CCTCAGCATTTCTTGTCCAT+CGG 0.524241 2:-11561364 None:intergenic
CCAAATATGCTGCATGAGCA+TGG 0.525898 2:-11559879 None:intergenic
TACCTCCTCGGTCAAAATGT+TGG 0.526158 2:-11560136 None:intergenic
TTGGCTGTAAGGCTTATTTG+AGG 0.528311 2:+11560007 MsaT006928.1:CDS
AGATTTGTGTGTTCGAAGGA+GGG 0.530744 2:+11559511 MsaT006928.1:CDS
TCTTTGTAGACACGTGTTGA+GGG 0.535253 2:+11561292 MsaT006928.1:CDS
TTGAAATTAATAACACCAAA+GGG 0.535464 2:-11559763 None:intergenic
TTTCTTGTGATCCATACATA+GGG 0.536566 2:+11559365 MsaT006928.1:CDS
TGTATGAGAAGCGTGCATCC+TGG 0.538953 2:+11560829 MsaT006928.1:CDS
ATTTGGGATAATGATTGTAA+AGG 0.539019 2:+11559282 None:intergenic
TCGGTCAAAATGTTGGAAGC+AGG 0.543963 2:-11560129 None:intergenic
TAGAAGATTTGTGTGTTCGA+AGG 0.548021 2:+11559507 MsaT006928.1:CDS
TCGTGAAGAAGAAAGAAAAG+AGG 0.548083 2:+11560992 MsaT006928.1:CDS
AAGATTTGTGTGTTCGAAGG+AGG 0.548897 2:+11559510 MsaT006928.1:CDS
GGAAGAAAATGCTTGAAGTA+GGG 0.553026 2:+11559722 MsaT006928.1:CDS
GCATCTAAATACATAGATGC+TGG 0.554139 2:+11561467 MsaT006928.1:CDS
TCGACTACCCACTTTCCTGA+TGG 0.556567 2:-11560042 None:intergenic
TGGTTGTCCAGCATTCATAA+GGG 0.557324 2:+11559555 MsaT006928.1:CDS
GATTTAAAATCTATGAAGAA+GGG 0.574074 2:+11561147 MsaT006928.1:CDS
AATTCCTATGAAAGAAAGCA+TGG 0.575352 2:+11560887 MsaT006928.1:CDS
AGATATTTGGTGCGTAAGTG+TGG 0.576360 2:+11561179 MsaT006928.1:CDS
GAATCAACCATCAGGAAAGT+GGG 0.580534 2:+11560035 MsaT006928.1:CDS
AAGAACTTGAAGTACAGGCC+AGG 0.583073 2:+11559800 MsaT006928.1:CDS
GGAGAAAACTGTGTTGGCAC+AGG 0.583735 2:+11561543 MsaT006928.1:CDS
TGTCAGATGTTTGAATATGA+AGG 0.583906 2:+11561266 MsaT006928.1:CDS
CCTTACAGCCAACTCTTGAA+GGG 0.585947 2:-11559996 None:intergenic
GCTTTGTGTGCTCAAAAGAA+GGG 0.587200 2:+11559965 MsaT006928.1:CDS
CTCGCTACATCTTACACCGA+TGG 0.590796 2:+11561348 MsaT006928.1:CDS
ATTGTAAAGGAGCATCCTGT+TGG 0.597584 2:+11559295 MsaT006928.1:CDS
GCCAGGATCGAACCATATGT+TGG 0.601674 2:+11559817 MsaT006928.1:CDS
AATGTGCCCTTTAAATAGAA+CGG 0.602152 2:-11560855 None:intergenic
CTTTAAATAGAACGGAACCC+AGG 0.602743 2:-11560847 None:intergenic
GGTAATGAGTAATGCAATTG+GGG 0.604332 2:+11559327 MsaT006928.1:CDS
ATGAGCATGGTAAAATTGGT+AGG 0.604922 2:-11559866 None:intergenic
AGCATGGAATCATTCTTTGG+TGG 0.604989 2:+11560903 MsaT006928.1:CDS
GCAAAGGAACAAGAGCTCGC+CGG 0.611525 2:+11560672 MsaT006928.1:intron
AAAATGCTTGAAGTAGGGAA+TGG 0.612218 2:+11559727 MsaT006928.1:CDS
CTTGAAATTAATAACACCAA+AGG 0.620334 2:-11559764 None:intergenic
TACGCACCAAATATCTGCTG+AGG 0.623181 2:-11561172 None:intergenic
CGCGGAATGAACTCTGTGAG+AGG 0.627384 2:-11560945 None:intergenic
AGGGTGGGAGAAAACTGTGT+TGG 0.629447 2:+11561537 MsaT006928.1:CDS
CTGAAGTTACTCGCAAGCCA+AGG 0.631542 2:+11559701 MsaT006928.1:CDS
GAACCCATTACCCATCAGAC+CGG 0.633308 2:-11560691 None:intergenic
CGGTGTAAGATGTAGCGAGA+AGG 0.633470 2:-11561344 None:intergenic
ACGCACCAAATATCTGCTGA+GGG 0.642827 2:-11561171 None:intergenic
TCGATGAATTTGACACCTCG+TGG 0.655612 2:+11560754 MsaT006928.1:CDS
AATGAGTAATGCAATTGGGG+AGG 0.656176 2:+11559330 MsaT006928.1:CDS
TGGACAAGAAATGCTGAGGA+TGG 0.668666 2:+11561368 MsaT006928.1:CDS
CCGATGGACAAGAAATGCTG+AGG 0.681471 2:+11561364 MsaT006928.1:CDS
TCGAGACCTCTTTCATAACG+CGG 0.715495 2:-11560963 None:intergenic
GACGAGTACAGCACTCCACG+AGG 0.746656 2:-11560769 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 11559292 11561568 11559292 ID=MsaG006928
Chr2 mRNA 11559292 11561568 11559292 ID=MsaT006928.1;Parent=MsaG006928
Chr2 exon 11559292 11560155 11559292 ID=MsaT006928.1.exon1;Parent=MsaT006928.1
Chr2 CDS 11559292 11560155 11559292 ID=cds.MsaT006928.1;Parent=MsaT006928.1
Chr2 exon 11560678 11561568 11560678 ID=MsaT006928.1.exon2;Parent=MsaT006928.1
Chr2 CDS 11560678 11561568 11560678 ID=cds.MsaT006928.1;Parent=MsaT006928.1
Gene Sequence

>MsaT006928.1

ATGATTGTAAAGGAGCATCCTGTTGGTACTGAACTGGTAATGAGTAATGCAATTGGGGAGGAGGAAATTGATTTTTCTTGTGATCCATACATAGGGTTAGAGTTTTTTAATGCGGATGACGCGCTTAAGTTTTATATATCTTATGGTAATCGAATGGGATTTAAAGTTCGGATTGGTCAACTTTATCGATCGAGAACTAATGGGTCTGTTTCTTCTAGAAGATTTGTGTGTTCGAAGGAGGGACATCAGCTCAGTTCAAGAACTGGTTGTCCAGCATTCATAAGGGTTCAGTTAAATGATTCTGGAAAGTGGGTTGTTGATCATTTTCATAAGGATCATAACCACACTCTTGAAAATGAAAGTGAAAGTTTCGCTCCAACATTGCAGCCAAAAGCTATTGAGTCTTCTACTGAAGTTACTCGCAAGCCAAGGAAGAAAATGCTTGAAGTAGGGAATGGTGAACCTATTTCTCCCTTTGGTGTTATTAATTTCAAGCGTATGAGAAAAGAAGAACTTGAAGTACAGGCCAGGATCGAACCATATGTTGGTCAAGAGTTCAGTACTCCAAATGAAGCCTACCAATTTTACCATGCTCATGCAGCATATTTGGGTTTTGGAATTCGAGTCGGTCAATTGTTTCGTTCTAAAAATGACGGATCAATTACATCTCGACGCTTTGTGTGCTCAAAAGAAGGGTTCCAGCACCCTTCAAGAGTTGGCTGTAAGGCTTATTTGAGGATTAAGAATCAACCATCAGGAAAGTGGGTAGTCGACCGTCTTGAAAAAGATCATAATCATGATCTGGGCCTTGAAAAGGAACGTAAGACAAAAAGTCTTCCTGCTTCCAACATTTTGACCGAGGAGGAACAAGAGCTCGCCGGTCTGATGGGTAATGGGTTCACTAAAGATTATGAAAAGTGTGTTTACCAGAGCCAGACAGTCGATGAATTTGACACCTCGTGGAGTGCTGTACTCGTCAAATATGGATTGAAGGACAATGCTTGGCTAAAAGAAATGTATGAGAAGCGTGCATCCTGGGTTCCGTTCTATTTAAAGGGCACATTTTCTGCTGGAATTCCTATGAAAGAAAGCATGGAATCATTCTTTGGTGGAAGTTTAAATGGCCAAACACCTCTCACAGAGTTCATTCCGCGTTATGAAAGAGGTCTCGAGCGACGTCGTGAAGAAGAAAGAAAAGAGGATTTTAATACTTCCAATTTTCAAACTTTTTTGCAAACAAAAGAGCCAGTAGAAGAACAGTGCAGAAGACTTTACACTCTTGCAGTATTCAAAGTATTTCAGAAAGAGCTTTTGCAGTGCTTTAGTTATCTTGGATTTAAAATCTATGAAGAAGGGTCCCTCAGCAGATATTTGGTGCGTAAGTGTGGAAATGATGATATGGTGAAGCATGTAGTTACATTCAATGCATCCAATCTTGACATTAGTTGTAGCTGTCAGATGTTTGAATATGAAGGTGTTCTTTGTAGACACGTGTTGAGGGTTTTTCAAATATTGGAAGTAAGAGAAGTTCCTTCTCGCTACATCTTACACCGATGGACAAGAAATGCTGAGGATGGTGTTTTTCCTGACATAGGATCATGGAGTAGCTCTCAGGAGTTAAAAAGTTTGATGCTATGGAGTCTAAGAGAAACAGCATCTAAATACATAGATGCTGGTGCATCATCAATTGAAAAGTATAAACTTGCCTATGAGATTTTGCGAGAGGGTGGGAGAAAACTGTGTTGGCACAGGTGA
Protein sequence

>MsaT006928.1

MIVKEHPVGTELVMSNAIGEEEIDFSCDPYIGLEFFNADDALKFYISYGNRMGFKVRIGQLYRSRTNGSVSSRRFVCSKEGHQLSSRTGCPAFIRVQLNDSGKWVVDHFHKDHNHTLENESESFAPTLQPKAIESSTEVTRKPRKKMLEVGNGEPISPFGVINFKRMRKEELEVQARIEPYVGQEFSTPNEAYQFYHAHAAYLGFGIRVGQLFRSKNDGSITSRRFVCSKEGFQHPSRVGCKAYLRIKNQPSGKWVVDRLEKDHNHDLGLEKERKTKSLPASNILTEEEQELAGLMGNGFTKDYEKCVYQSQTVDEFDTSWSAVLVKYGLKDNAWLKEMYEKRASWVPFYLKGTFSAGIPMKESMESFFGGSLNGQTPLTEFIPRYERGLERRREEERKEDFNTSNFQTFLQTKEPVEEQCRRLYTLAVFKVFQKELLQCFSYLGFKIYEEGSLSRYLVRKCGNDDMVKHVVTFNASNLDISCSCQMFEYEGVLCRHVLRVFQILEVREVPSRYILHRWTRNAEDGVFPDIGSWSSSQELKSLMLWSLRETASKYIDAGASSIEKYKLAYEILREGGRKLCWHR*