AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007030


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007030 MtrunA17_Chr8g0363841 76.712 146 34 0 1 146 1 146 3.34e-75 243
MsaG007030 MtrunA17_Chr8g0363841 43.750 208 64 3 148 302 436 643 1.68e-43 158
MsaG007030 MtrunA17_Chr2g0329361 28.025 157 89 6 18 154 62 214 2.44e-12 65.9
MsaG007030 MtrunA17_Chr7g0258861 34.677 124 71 4 19 137 12 130 1.47e-11 64.3
MsaG007030 MtrunA17_Chr5g0448761 39.773 88 48 3 62 144 104 191 2.65e-11 64.3
MsaG007030 MtrunA17_Chr4g0033611 27.815 151 105 3 14 162 73 221 3.84e-11 62.4
MsaG007030 MtrunA17_Chr5g0440451 29.365 126 76 2 18 143 44 156 5.07e-11 63.5
MsaG007030 MtrunA17_Chr2g0329421 28.972 107 75 1 18 124 28 133 6.49e-11 61.2
MsaG007030 MtrunA17_Chr2g0287061 33.036 112 62 2 7 118 173 271 7.98e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007030 AT4G38180.1 42.529 87 46 2 62 144 117 203 4.78e-14 73.2
MsaG007030 AT3G59470.3 32.039 103 69 1 18 120 71 172 4.05e-11 62.8
MsaG007030 AT3G59470.1 32.039 103 69 1 18 120 71 172 4.05e-11 62.8
MsaG007030 AT3G59470.2 32.039 103 69 1 18 120 83 184 4.52e-11 62.8

Find 52 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCAGGAATCTTGTAATTT+TGG 0.216335 2:+12893447 MsaT007030.1:intron
ATTATTCTTAAACCCTTCTT+TGG 0.257903 2:-12891058 None:intergenic
TTTGGACTCATCCACTTCTA+TGG 0.267599 2:+12892435 MsaT007030.1:CDS
GTCTTTATACAATTTAGTTT+TGG 0.305862 2:+12892696 MsaT007030.1:CDS
ATGTTAGCAAGGAAGGAGTT+TGG 0.356508 2:+12891151 MsaT007030.1:CDS
GGGTTTAAGAATAATGAATA+TGG 0.371754 2:+12891066 MsaT007030.1:CDS
ACGGGACCTCATAAAGTCTA+TGG 0.389034 2:+12891269 MsaT007030.1:CDS
AACAGTAAAACTCTTTAAAA+CGG 0.389800 2:-12891035 None:intergenic
ATCACCATCAGTCATTGAAT+TGG 0.389903 2:-12892638 None:intergenic
GAAAAGGTGGCGACAATTAG+AGG 0.395021 2:+12892183 MsaT007030.1:CDS
ATGACTGATGGTGATGATTC+CGG 0.408403 2:+12892646 MsaT007030.1:CDS
TTTAGTGGCATTTGAGAAGA+TGG 0.409481 2:+12892159 MsaT007030.1:intron
CAAGATTTAGTGTAAGAAAA+GGG 0.432600 2:+12890977 MsaT007030.1:CDS
TGTCAATCAGTTGAAATTGA+TGG 0.438803 2:+12890874 MsaT007030.1:CDS
ACAAGATTTAGTGTAAGAAA+AGG 0.440176 2:+12890976 MsaT007030.1:CDS
AATTCACCATAGACTTTATG+AGG 0.446268 2:-12891275 None:intergenic
ACAGTAAAACTCTTTAAAAC+GGG 0.452807 2:-12891034 None:intergenic
GACATTTGACATATTTCTCA+TGG 0.454279 2:-12891244 None:intergenic
CTACGTTGATAATTGCTACT+TGG 0.456826 2:-12893480 None:intergenic
AAATATGTCAAATGTCAAAC+GGG 0.464386 2:+12891251 MsaT007030.1:CDS
CGCAACAAATGTCTTTGCTC+AGG 0.472523 2:-12891219 None:intergenic
CTTATGAAAGAGCTGATTCA+AGG 0.480001 2:+12891100 MsaT007030.1:CDS
GGAAGCACTTCCGTGGAGAT+TGG 0.486127 2:+12892343 MsaT007030.1:CDS
GAAATATGTCAAATGTCAAA+CGG 0.486335 2:+12891250 MsaT007030.1:CDS
AATGAGTATCTTAATGGGGC+AGG 0.495180 2:+12892319 MsaT007030.1:CDS
ACCATCAGTCATTGAATTGG+AGG 0.504486 2:-12892635 None:intergenic
CGTTGATAATTGCTACTTGG+TGG 0.516800 2:-12893477 None:intergenic
AGGTTTAATGTTAGCAAGGA+AGG 0.523844 2:+12891144 MsaT007030.1:CDS
AAGGACAAATTGCAAAGCTA+TGG 0.542290 2:+12891119 MsaT007030.1:CDS
ATTATGATCTAATACAAACT+TGG 0.563904 2:-12891181 None:intergenic
AGCGTTGCGGTAAAGAAGAC+CGG 0.568368 2:-12892665 None:intergenic
ATTTAGTGTAAGAAAAGGGA+GGG 0.568911 2:+12890981 MsaT007030.1:CDS
GGCGAGGTTTAATGTTAGCA+AGG 0.569412 2:+12891140 MsaT007030.1:CDS
TAATGAATATGGAGGTGAAG+TGG 0.572461 2:+12891077 MsaT007030.1:CDS
GAGTATCTTAATGGGGCAGG+TGG 0.572748 2:+12892322 MsaT007030.1:CDS
ACCTCCAATTCAATGACTGA+TGG 0.575513 2:+12892634 MsaT007030.1:CDS
GATTTAGTGTAAGAAAAGGG+AGG 0.580368 2:+12890980 MsaT007030.1:CDS
CTGAGCAAAGACATTTGTTG+CGG 0.581932 2:+12891220 MsaT007030.1:CDS
ACTGCAGCTAACCATAGAAG+TGG 0.582272 2:-12892446 None:intergenic
GGCAGGTGGAAGCACTTCCG+TGG 0.590405 2:+12892336 MsaT007030.1:CDS
ATAAAGTCTATGGTGAATTG+TGG 0.590947 2:+12891279 MsaT007030.1:CDS
CATTTGAGAAGATGGTGAAA+AGG 0.603784 2:+12892167 MsaT007030.1:CDS
TTTAAGAATAATGAATATGG+AGG 0.604513 2:+12891069 MsaT007030.1:CDS
TTGAGAAGATGGTGAAAAGG+TGG 0.621585 2:+12892170 MsaT007030.1:CDS
CAAATTGCAAAGCTATGGCG+AGG 0.632194 2:+12891124 MsaT007030.1:CDS
TTTAAATGAGTATCTTAATG+GGG 0.632939 2:+12892315 MsaT007030.1:CDS
TCACTACTGTTTATAACACG+AGG 0.639447 2:-12892241 None:intergenic
AACTCTACGACATGAATCTG+TGG 0.642020 2:+12892264 MsaT007030.1:CDS
AACAAATGTCTTTGCTCAGG+CGG 0.646674 2:-12891216 None:intergenic
ATCACATCGACCAATCTCCA+CGG 0.672242 2:-12892353 None:intergenic
CTACTGTTTATAACACGAGG+AGG 0.687262 2:-12892238 None:intergenic
AAGACTTCAGAATAGCGTTG+CGG 0.703840 2:-12892678 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 12890859 12893522 12890859 ID=MsaG007030
Chr2 mRNA 12890859 12893522 12890859 ID=MsaT007030.1;Parent=MsaG007030
Chr2 exon 12890859 12891300 12890859 ID=MsaT007030.1.exon1;Parent=MsaT007030.1
Chr2 CDS 12890859 12891300 12890859 ID=cds.MsaT007030.1;Parent=MsaT007030.1
Chr2 exon 12892164 12892456 12892164 ID=MsaT007030.1.exon2;Parent=MsaT007030.1
Chr2 CDS 12892164 12892456 12892164 ID=cds.MsaT007030.1;Parent=MsaT007030.1
Chr2 exon 12892616 12892752 12892616 ID=MsaT007030.1.exon3;Parent=MsaT007030.1
Chr2 CDS 12892616 12892752 12892616 ID=cds.MsaT007030.1;Parent=MsaT007030.1
Chr2 exon 12893453 12893522 12893453 ID=MsaT007030.1.exon4;Parent=MsaT007030.1
Chr2 CDS 12893453 12893522 12893453 ID=cds.MsaT007030.1;Parent=MsaT007030.1
Gene Sequence

>MsaT007030.1

ATGGAGATAAATGAATGTCAATCAGTTGAAATTGATGGATGCAAAGATATTTTTCTTGGTCAAGTTGTGAATAACAAAGATGAAGCTTATAATTTATATCAAGCACATGCCTTCAAAACAAGATTTAGTGTAAGAAAAGGGAGGGAGTTATATTATGATAATGAGAGAAAAATCACCCGTTTTAAAGAGTTTTACTGTTCCAAAGAAGGGTTTAAGAATAATGAATATGGAGGTGAAGTGGCTTATGAAAGAGCTGATTCAAGGACAAATTGCAAAGCTATGGCGAGGTTTAATGTTAGCAAGGAAGGAGTTTGGAAAGTTACCAAGTTTGTATTAGATCATAATCATCAACTTGTACCGCCTGAGCAAAGACATTTGTTGCGGTCCATGAGAAATATGTCAAATGTCAAACGGGACCTCATAAAGTCTATGGTGAATTGTGTGGCATTTGAGAAGATGGTGAAAAGGTGGCGACAATTAGAGGTTGAGAACGAATTCAAAAATAGTCAGAGTGCGCCTCCTCGTGTTATAAACAGTAGTGAAACTCTACGACATGAATCTGTGGTATATACTCACAAAATCTTTACACTTTTTTTAAATGAGTATCTTAATGGGGCAGGTGGAAGCACTTCCGTGGAGATTGGTCGATGTGATAATGTGTCATATCATGAAGTTGTGTTGAACATCATCCCCAATAAAAAGTATATCGTGACTTTGGACTCATCCACTTCTATGAGTTTAGAAGAAGATTCTACCTCCAATTCAATGACTGATGGTGATGATTCCGGTCTTCTTTACCGCAACGCTATTCTGAAGTCTTTATACAATTTAGTTTTGGAATCCCAGGAGGACAAAAAAGCACAAAAAATCATGAATCTTGTAATTTTGGTCGGATACCACCAAGTAGCAATTATCAACGTAGACAATGATCAAGTGATCTGA

Protein sequence

>MsaT007030.1

MEINECQSVEIDGCKDIFLGQVVNNKDEAYNLYQAHAFKTRFSVRKGRELYYDNERKITRFKEFYCSKEGFKNNEYGGEVAYERADSRTNCKAMARFNVSKEGVWKVTKFVLDHNHQLVPPEQRHLLRSMRNMSNVKRDLIKSMVNCVAFEKMVKRWRQLEVENEFKNSQSAPPRVINSSETLRHESVVYTHKIFTLFLNEYLNGAGGSTSVEIGRCDNVSYHEVVLNIIPNKKYIVTLDSSTSMSLEEDSTSNSMTDGDDSGLLYRNAILKSLYNLVLESQEDKKAQKIMNLVILVGYHQVAIINVDNDQVI*