AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007039


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007039 MtrunA17_Chr2g0288481 85.214 257 25 3 1 257 1 244 3.10e-149 416
MsaG007039 MtrunA17_Chr7g0272991 48.370 184 88 6 69 251 82 259 2.97e-51 168
MsaG007039 MtrunA17_Chr2g0310741 53.947 152 54 4 70 217 85 224 4.35e-50 165
MsaG007039 MtrunA17_Chr8g0376351 88.372 86 9 1 106 190 1 86 2.04e-48 155
MsaG007039 MtrunA17_Chr1g0191681 50.602 166 67 6 69 230 82 236 3.15e-48 160
MsaG007039 MtrunA17_Chr3g0132191 50.336 149 58 2 70 218 37 169 8.68e-43 145
MsaG007039 MtrunA17_Chr2g0318881 58.442 77 31 1 89 164 23 99 3.48e-23 91.3
MsaG007039 MtrunA17_Chr2g0318921 58.442 77 31 1 89 164 23 99 3.82e-23 90.9
MsaG007039 MtrunA17_Chr3g0081161 42.308 78 45 0 89 166 9 86 3.35e-12 65.1
MsaG007039 MtrunA17_Chr8g0354151 39.744 78 47 0 89 166 9 86 1.27e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007039 AT1G54830.2 52.500 160 70 3 69 224 51 208 4.50e-49 161
MsaG007039 AT1G54830.3 52.500 160 70 3 69 224 51 208 4.50e-49 161
MsaG007039 AT1G54830.1 52.500 160 70 3 69 224 51 208 4.50e-49 161
MsaG007039 AT1G08970.3 51.765 170 65 6 70 237 62 216 1.17e-47 158
MsaG007039 AT1G08970.4 51.765 170 65 6 70 237 62 216 1.17e-47 158
MsaG007039 AT1G08970.2 51.765 170 65 6 70 237 62 216 1.17e-47 158
MsaG007039 AT1G08970.1 51.765 170 65 6 70 237 62 216 1.17e-47 158
MsaG007039 AT1G56170.1 57.812 128 49 3 67 192 55 179 3.85e-47 155
MsaG007039 AT1G56170.2 57.812 128 49 3 67 192 55 179 3.85e-47 155
MsaG007039 AT3G48590.1 51.678 149 63 2 70 218 47 186 6.50e-46 154
MsaG007039 AT5G63470.2 56.250 128 53 1 70 194 60 187 1.03e-43 149
MsaG007039 AT5G63470.1 56.250 128 53 1 70 194 60 187 1.03e-43 149
MsaG007039 AT5G50480.1 46.250 160 69 5 66 223 35 179 1.68e-37 131
MsaG007039 AT5G27910.1 42.012 169 77 6 65 231 16 165 8.78e-32 115
MsaG007039 AT5G50490.1 45.192 104 54 1 65 168 16 116 1.39e-25 99.8
MsaG007039 AT5G50470.1 41.085 129 67 2 70 192 48 173 1.10e-24 98.2
MsaG007039 AT5G38140.1 39.806 103 60 1 63 165 44 144 1.34e-21 89.7

Find 53 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGCTTAATCTTCTGAATT+TGG 0.149271 2:+13035070 None:intergenic
ATCTTCTTAATCCTTGTTAA+AGG 0.176954 2:+13035559 None:intergenic
ATTGGGACACTTTCTCTTCT+TGG 0.187927 2:+13035292 None:intergenic
AATGGAGCGTGACATATTTA+TGG 0.230451 2:-13035320 MsaT007039.1:CDS
GGCGAGGCACTGATACTATT+TGG 0.252757 2:+13035758 None:intergenic
TGGTCCTCCAAGAATGCTTA+TGG 0.263320 2:-13035200 MsaT007039.1:CDS
GGCATGTGAAATGTTTATTA+TGG 0.268895 2:-13035476 MsaT007039.1:CDS
TGTCTTCCCATAAGCATTCT+TGG 0.296015 2:+13035193 None:intergenic
GTTGTCGGTGAGAGATTTCT+TGG 0.314048 2:+13035677 None:intergenic
GAATTTGGCAAATCAGTAGA+AGG 0.372595 2:+13035085 None:intergenic
GTGCCACCTCAGTATGTTGC+AGG 0.380885 2:-13035232 MsaT007039.1:CDS
TAATAAGGAACATTCCCTAT+TGG 0.390711 2:+13035274 None:intergenic
ACAGACATAGGCAGATAATA+AGG 0.391390 2:+13035259 None:intergenic
GGAAACATTTGATTTGCAAA+AGG 0.397276 2:+13035130 None:intergenic
AATAAGGAACATTCCCTATT+GGG 0.400669 2:+13035275 None:intergenic
CCCGTTGTAAGGAGGTTGAT+TGG 0.412220 2:+13035165 None:intergenic
AGAAGAGAAAGTGTCCCAAT+AGG 0.412571 2:-13035289 MsaT007039.1:CDS
GAAACATTTGATTTGCAAAA+GGG 0.424540 2:+13035131 None:intergenic
ACTGTTGATTTCAAAAGTAA+TGG 0.437192 2:-13035586 MsaT007039.1:CDS
ATGGAGCGTGACATATTTAT+GGG 0.438860 2:-13035319 MsaT007039.1:CDS
GAAGAGAAAGTGTCCCAATA+GGG 0.454022 2:-13035288 MsaT007039.1:CDS
TCTACTCAGGTATGATGAAA+AGG 0.465576 2:+13035708 None:intergenic
GGTCCTCCAAGAATGCTTAT+GGG 0.469493 2:-13035199 MsaT007039.1:CDS
CCGTTGTAAGGAGGTTGATT+GGG 0.477387 2:+13035166 None:intergenic
CCAATCAACCTCCTTACAAC+GGG 0.477395 2:-13035165 MsaT007039.1:CDS
CATGCCTTAGCAAACAGAAT+AGG 0.486382 2:+13035493 None:intergenic
CCTAGTTGATATTGTCCCTA+GGG 0.487741 2:-13035350 MsaT007039.1:CDS
TGGAGCTAGCAACAAGGTCA+TGG 0.501489 2:-13035456 MsaT007039.1:CDS
CCCTAGGGACAATATCAACT+AGG 0.511793 2:+13035350 None:intergenic
TGTGATGGATGCCCGTTGTA+AGG 0.514096 2:+13035154 None:intergenic
TATGTTGCAGGTCCATCATA+TGG 0.515120 2:-13035220 MsaT007039.1:CDS
CTTGGAGTTGAACTCTACTC+AGG 0.515545 2:+13035695 None:intergenic
AAGATCATGAAAGCAGAAGA+AGG 0.528329 2:-13035541 MsaT007039.1:CDS
GGCACCTATTCTGTTTGCTA+AGG 0.530640 2:-13035497 MsaT007039.1:CDS
TGATTTGCAAAAGGGTGTGA+TGG 0.553637 2:+13035139 None:intergenic
TCCTAGTTGATATTGTCCCT+AGG 0.558927 2:-13035351 MsaT007039.1:CDS
CTTCCCATAAGCATTCTTGG+AGG 0.580142 2:+13035196 None:intergenic
GTACTTGCAGTGTGAAACAA+AGG 0.581978 2:+13035736 None:intergenic
GGAGCTAGCAACAAGGTCAT+GGG 0.582315 2:-13035455 MsaT007039.1:CDS
CCCAATCAACCTCCTTACAA+CGG 0.599569 2:-13035166 MsaT007039.1:CDS
ATTCTTGGAGGACCATATGA+TGG 0.609961 2:+13035208 None:intergenic
GTAATGGCTTACCTTTAACA+AGG 0.611130 2:-13035570 MsaT007039.1:CDS
TACTTGCAGTGTGAAACAAA+GGG 0.618670 2:+13035737 None:intergenic
GGTGGCACATGAACAGACAT+AGG 0.629233 2:+13035247 None:intergenic
AAGGTCATGGGCTAATACAG+AGG 0.637560 2:-13035443 MsaT007039.1:CDS
GCAGTGTGAAACAAAGGGCG+AGG 0.647228 2:+13035742 None:intergenic
AATTTGGCAAATCAGTAGAA+GGG 0.648246 2:+13035086 None:intergenic
GATGGATGCCCGTTGTAAGG+AGG 0.657482 2:+13035157 None:intergenic
AGTGAGTATGATATCAGCTG+AGG 0.690629 2:-13035518 MsaT007039.1:CDS
TTATTATGGAGCTAGCAACA+AGG 0.697305 2:-13035462 MsaT007039.1:CDS
GGACCTGCAACATACTGAGG+TGG 0.712994 2:+13035229 None:intergenic
GATGGACCTGCAACATACTG+AGG 0.735823 2:+13035226 None:intergenic
AAAGACACAAGTGAACCCAG+AGG 0.809094 2:-13035818 MsaT007039.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 13035075 13035848 13035075 ID=MsaG007039
Chr2 mRNA 13035075 13035848 13035075 ID=MsaT007039.1;Parent=MsaG007039
Chr2 exon 13035075 13035848 13035075 ID=MsaT007039.1.exon1;Parent=MsaT007039.1
Chr2 CDS 13035075 13035848 13035075 ID=cds.MsaT007039.1;Parent=MsaT007039.1
Gene Sequence

>MsaT007039.1

ATGGATCAAAAGACACAAGTGAACCCAGAGGAAAAAAGGAATGATACACCAACACAAAATCAATCAAACCAAATAGTATCAGTGCCTCGCCCTTTGTTTCACACTGCAAGTACTTCTGCCTTTTCATCATACCTGAGTAGAGTTCAACTCCAAGAAATCTCTCACCGACAACAAAAACAACAAGAATTGCAGAAAAAACTTGGCACCTTCTGGGCAAAACAAAATGAAGAAGTTCAGAAGACTGTTGATTTCAAAAGTAATGGCTTACCTTTAACAAGGATTAAGAAGATCATGAAAGCAGAAGAAGGAGTGAGTATGATATCAGCTGAGGCACCTATTCTGTTTGCTAAGGCATGTGAAATGTTTATTATGGAGCTAGCAACAAGGTCATGGGCTAATACAGAGGTGAACAAAAGAAAGACACTTCAAAAGAGTGACATTGCATCTGCTGTCTCAAGTAATGAAGTGTTTGATTTCCTAGTTGATATTGTCCCTAGGGAAAAAACAATGGAGCGTGACATATTTATGGGTATACCAAGAAGAGAAAGTGTCCCAATAGGGAATGTTCCTTATTATCTGCCTATGTCTGTTCATGTGCCACCTCAGTATGTTGCAGGTCCATCATATGGTCCTCCAAGAATGCTTATGGGAAGACATCTTCCCAATCAACCTCCTTACAACGGGCATCCATCACACCCTTTTGCAAATCAAATGTTTCCAATCCCAAAGAAAGAAAACGACCCTTCTACTGATTTGCCAAATTCAGAAGATTAA

Protein sequence

>MsaT007039.1

MDQKTQVNPEEKRNDTPTQNQSNQIVSVPRPLFHTASTSAFSSYLSRVQLQEISHRQQKQQELQKKLGTFWAKQNEEVQKTVDFKSNGLPLTRIKKIMKAEEGVSMISAEAPILFAKACEMFIMELATRSWANTEVNKRKTLQKSDIASAVSSNEVFDFLVDIVPREKTMERDIFMGIPRRESVPIGNVPYYLPMSVHVPPQYVAGPSYGPPRMLMGRHLPNQPPYNGHPSHPFANQMFPIPKKENDPSTDLPNSED*