AlfalfaGEDB | MsaG007048

M. sativa ssp. caerulea / MsaG007048

KEGG: -
GO: GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007048	XP_013462843.2	86.124	209	25	3	5	210	47	254	8.13e-123	358

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007048	sp\|Q700D9\|MYBF_ARATH	72.581	62	16	1	19	79	15	76	7.15e-22	93.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007048	tr\|A0A072V6D0\|A0A072V6D0_MEDTR	86.124	209	25	3	5	210	31	238	5.44e-123	357

TFs/TRs:

Gene ID	Type	Classification
MsaG007048	TF	GARP-G2-like

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsaG002826	MsaG007048	0.812305	7.789778e-51	1.407523e-48
MsaG007048	MsaG032531	0.813264	4.814656e-51	8.910048e-49
MsaG007048	MsaG033878	0.814253	2.923548e-51	5.546161e-49
MsaG007048	MsaG044703	0.801254	1.645483e-48	2.288622e-46
MsaG007048	MsaG018616	0.810666	1.761419e-50	3.057215e-48
MsaG007048	MsaG033152	0.802148	1.080556e-48	1.533755e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007048	MtrunA17_Chr2g0288601	73.684	76	17	2	5	77	47	122	1.79e-30	108
MsaG007048	MtrunA17_Chr1g0210911	67.500	80	25	1	6	84	1	80	1.10e-28	109
MsaG007048	MtrunA17_Chr7g0243081	76.812	69	15	1	15	82	59	127	5.92e-28	108
MsaG007048	MtrunA17_Chr2g0320231	61.446	83	31	1	15	96	85	167	3.03e-26	103
MsaG007048	MtrunA17_Chr1g0162931	72.727	66	17	1	18	82	9	74	3.40e-26	102
MsaG007048	MtrunA17_Chr8g0379121	68.056	72	22	1	12	82	96	167	9.95e-26	103
MsaG007048	MtrunA17_Chr2g0283951	57.143	84	31	2	8	86	70	153	3.46e-21	90.1
MsaG007048	MtrunA17_Chr1g0154261	72.000	50	14	0	33	82	133	182	8.41e-18	79.7
MsaG007048	MtrunA17_Chr8g0366281	57.971	69	23	1	14	82	167	229	2.50e-17	79.0
MsaG007048	MtrunA17_Chr3g0095611	47.436	78	40	1	11	87	3	80	1.16e-16	75.1
MsaG007048	MtrunA17_Chr5g0410741	60.345	58	23	0	33	90	226	283	1.22e-16	77.4
MsaG007048	MtrunA17_Chr6g0479431	58.333	60	25	0	35	94	211	270	2.01e-16	76.6
MsaG007048	MtrunA17_Chr3g0109981	68.750	48	15	0	35	82	283	330	1.23e-15	74.7
MsaG007048	MtrunA17_Chr7g0274471	56.452	62	25	1	34	93	226	287	7.97e-15	72.4
MsaG007048	MtrunA17_Chr7g0261911	41.304	92	47	2	14	104	256	341	9.31e-15	72.4
MsaG007048	MtrunA17_Chr1g0190301	61.224	49	19	0	34	82	183	231	4.17e-14	70.1
MsaG007048	MtrunA17_Chr7g0258551	63.265	49	18	0	34	82	220	268	1.18e-13	68.9
MsaG007048	MtrunA17_Chr1g0197171	55.932	59	23	1	28	83	43	101	1.51e-13	67.8
MsaG007048	MtrunA17_Chr7g0254981	45.055	91	47	2	33	121	57	146	1.56e-13	67.8
MsaG007048	MtrunA17_Chr5g0409731	58.182	55	20	1	28	79	24	78	2.61e-13	67.4
MsaG007048	MtrunA17_Chr1g0175091	58.333	60	23	1	34	93	266	323	3.74e-13	67.4
MsaG007048	MtrunA17_Chr2g0290991	60.000	50	20	0	34	83	47	96	9.89e-13	65.9
MsaG007048	MtrunA17_Chr2g0291021	63.265	49	18	0	34	82	57	105	1.36e-12	65.5
MsaG007048	MtrunA17_Chr6g0461641	60.870	46	18	0	34	79	50	95	2.45e-12	65.1
MsaG007048	MtrunA17_Chr1g0175081	53.448	58	25	1	34	91	30	85	5.26e-12	60.1
MsaG007048	MtrunA17_Chr2g0310011	41.667	84	42	2	33	110	42	124	9.89e-12	63.2
MsaG007048	MtrunA17_Chr2g0321461	47.945	73	31	2	7	79	33	98	1.45e-11	62.8
MsaG007048	MtrunA17_Chr8g0356501	49.153	59	27	1	28	83	36	94	2.05e-11	62.0
MsaG007048	MtrunA17_Chr5g0417521	49.057	53	27	0	34	86	23	75	3.34e-11	61.6
MsaG007048	MtrunA17_Chr4g0042451	47.368	57	29	1	34	90	25	80	6.38e-11	60.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsaG007048	AT4G04605.1	50.459	109	48	3	15	121	2	106	4.50e-28	105
MsaG007048	AT2G38300.1	56.481	108	42	3	15	117	42	149	3.37e-27	106
MsaG007048	AT2G02060.2	77.419	62	13	1	19	79	21	82	6.12e-26	101
MsaG007048	AT2G02060.1	77.419	62	13	1	19	79	21	82	6.12e-26	101
MsaG007048	AT2G42660.1	43.972	141	73	3	19	157	42	178	1.71e-24	97.4
MsaG007048	AT2G40260.1	66.667	72	23	1	19	89	74	145	2.29e-24	99.4
MsaG007048	AT1G14600.1	72.581	62	16	1	19	79	15	76	7.27e-23	93.2
MsaG007048	AT4G04555.1	55.556	72	31	1	15	85	2	73	2.05e-20	82.8
MsaG007048	AT4G04580.1	48.810	84	39	2	18	100	5	85	6.45e-18	77.8
MsaG007048	AT5G42630.2	51.852	81	33	1	2	82	87	161	1.23e-17	78.6
MsaG007048	AT5G42630.1	51.852	81	33	1	2	82	87	161	1.27e-17	79.3
MsaG007048	AT4G17695.1	68.000	50	16	0	33	82	171	220	1.44e-16	77.0
MsaG007048	AT1G32240.2	68.000	50	16	0	33	82	220	269	3.12e-16	76.6
MsaG007048	AT1G32240.1	68.000	50	16	0	33	82	220	269	3.26e-16	76.6
MsaG007048	AT5G16560.1	66.000	50	17	0	33	82	226	275	1.59e-15	74.7
MsaG007048	AT4G28610.1	63.265	49	18	0	34	82	233	281	5.84e-14	70.1
MsaG007048	AT5G29000.1	63.265	49	18	0	34	82	196	244	6.21e-14	70.1
MsaG007048	AT5G29000.3	63.265	49	18	0	34	82	196	244	6.21e-14	70.1
MsaG007048	AT5G29000.2	63.265	49	18	0	34	82	239	287	6.36e-14	70.1
MsaG007048	AT5G29000.4	63.265	49	18	0	34	82	239	287	6.38e-14	69.7
MsaG007048	AT3G04450.4	59.184	49	20	0	34	82	206	254	9.54e-14	69.7
MsaG007048	AT3G04450.2	59.184	49	20	0	34	82	206	254	9.54e-14	69.7
MsaG007048	AT3G04450.3	59.184	49	20	0	34	82	246	294	9.99e-14	68.9
MsaG007048	AT3G04450.1	59.184	49	20	0	34	82	246	294	1.13e-13	69.3
MsaG007048	AT5G45580.1	53.333	60	25	1	28	84	6	65	6.87e-13	66.2
MsaG007048	AT5G45580.2	53.333	60	25	1	28	84	22	81	6.91e-13	66.2
MsaG007048	AT2G01060.1	63.265	49	18	0	34	82	23	71	9.01e-13	66.2
MsaG007048	AT3G13040.3	46.667	75	40	0	34	108	249	323	9.22e-13	66.6
MsaG007048	AT3G13040.1	46.667	75	40	0	34	108	249	323	9.22e-13	66.6
MsaG007048	AT3G13040.2	46.667	75	40	0	34	108	249	323	9.22e-13	66.6
MsaG007048	AT3G12730.1	52.542	59	25	1	28	83	22	80	1.43e-12	65.1
MsaG007048	AT2G20400.2	61.224	49	19	0	34	82	239	287	1.86e-12	65.9
MsaG007048	AT2G20400.1	61.224	49	19	0	34	82	239	287	1.89e-12	65.9
MsaG007048	AT2G06020.1	55.319	47	21	0	33	79	93	139	2.21e-12	65.1
MsaG007048	AT5G06800.2	50.000	74	30	2	34	100	200	273	3.08e-12	65.1
MsaG007048	AT5G06800.3	61.224	49	19	0	34	82	200	248	4.89e-12	63.9
MsaG007048	AT3G24120.2	49.153	59	27	1	28	83	40	98	6.60e-12	63.5
MsaG007048	AT3G24120.1	49.153	59	27	1	28	83	40	98	6.93e-12	63.5
MsaG007048	AT5G06800.1	61.224	49	19	0	34	82	200	248	9.21e-12	63.5
MsaG007048	AT3G04030.1	56.522	46	20	0	34	79	53	98	1.74e-11	62.8
MsaG007048	AT5G18240.2	58.696	46	19	0	34	79	53	98	1.76e-11	62.8
MsaG007048	AT5G18240.3	58.696	46	19	0	34	79	53	98	1.76e-11	62.8
MsaG007048	AT3G04030.3	56.522	46	20	0	34	79	53	98	2.01e-11	62.8
MsaG007048	AT5G18240.5	58.696	46	19	0	34	79	53	98	2.01e-11	62.8
MsaG007048	AT5G18240.1	58.696	46	19	0	34	79	53	98	2.01e-11	62.8
MsaG007048	AT5G18240.4	58.696	46	19	0	34	79	53	98	2.01e-11	62.8
MsaG007048	AT4G13640.2	47.458	59	28	1	28	83	36	94	2.29e-11	62.0
MsaG007048	AT4G13640.1	47.458	59	28	1	28	83	36	94	2.41e-11	62.0
MsaG007048	AT1G79430.2	49.153	59	27	1	28	83	33	91	2.57e-11	62.4

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTATTCCAGGGATGCTTAT+TGG	0.219949	2:+13177447	MsaT007048.1:intron
GAATCTTTGTTGGTCGCTTT+TGG	0.263083	2:-13178699	None:intergenic
GTTTGGAGAATTACATTCTT+TGG	0.276827	2:-13177292	None:intergenic
CACACTTACATCCTCTTTGA+TGG	0.313928	2:+13177539	MsaT007048.1:CDS
ATCCTCTTTGATGGTATTCT+CGG	0.352821	2:+13177548	MsaT007048.1:CDS
GGACCTCCTGAGCTTGTTGA+TGG	0.365393	2:-13178773	None:intergenic
TTATATCTTCGAATCTTTGT+TGG	0.384293	2:-13178709	None:intergenic
CAATCAATTGTTATCTGTAA+TGG	0.391540	2:+13177500	MsaT007048.1:CDS
ATTCACTCTCTCTCCTCTCC+TGG	0.393108	2:-13178732	None:intergenic
ATGAATTGTAAAACTAACTT+TGG	0.405423	2:-13175692	None:intergenic
TCATGTCAAAAGCCACCTTC+AGG	0.411764	2:+13175739	MsaT007048.1:CDS
TTTACAATTCATGAATATCA+AGG	0.423774	2:+13175703	MsaT007048.1:CDS
TATTGGCTAGAGTCTTTCAA+TGG	0.428858	2:+13177464	MsaT007048.1:CDS
CAATGTTATTGCAGATGTAC+AGG	0.430914	2:+13175934	MsaT007048.1:intron
GACCTCCTGAGCTTGTTGAT+GGG	0.440748	2:-13178772	None:intergenic
GTGCATGCAGTTCAAAGGCT+TGG	0.455826	2:+13175426	MsaT007048.1:CDS
ATCAAAGTTGCCTCGGCCGT+TGG	0.462681	2:+13175380	MsaT007048.1:CDS
AAACAAGAGCAGATGATTGA+AGG	0.472078	2:+13175963	MsaT007048.1:CDS
AGAGAGACATCATTTGTGTC+TGG	0.491055	2:-13178794	None:intergenic
GGCAACTTTGATCGAACATA+AGG	0.496488	2:-13175369	None:intergenic
CTGTCCCCATCAACAAGCTC+AGG	0.503401	2:+13178767	MsaT007048.1:CDS
CCAAGCTACTATCTGAATCA+TGG	0.514407	2:+13177269	MsaT007048.1:CDS
TTTACCAGCTGCAAAGAGAA+AGG	0.520218	2:+13177196	MsaT007048.1:intron
TTACAATTCATGAATATCAA+GGG	0.527486	2:+13175704	MsaT007048.1:CDS
GCTTTGTGCATGCAGTTCAA+AGG	0.532535	2:+13175421	MsaT007048.1:CDS
ACCTCCTGAGCTTGTTGATG+GGG	0.534570	2:-13178771	None:intergenic
ATTTACGTACTTGAACAGTC+TGG	0.542233	2:-13177574	None:intergenic
TTTAGATTCTCATCGGACTC+AGG	0.544361	2:+13175318	MsaT007048.1:CDS
CATGCAGTTCAAAGGCTTGG+AGG	0.546546	2:+13175429	MsaT007048.1:CDS
ATGTTCGATCAAAGTTGCCT+CGG	0.547165	2:+13175373	MsaT007048.1:CDS
GAGTCTTTCAATGGAACTCA+AGG	0.550479	2:+13177473	MsaT007048.1:CDS
GCTTGGAACAAGGGCTGAGG+AGG	0.553779	2:-13177252	None:intergenic
CCATGATTCAGATAGTAGCT+TGG	0.557206	2:-13177269	None:intergenic
GTTGCCTCGGCCGTTGGACA+CGG	0.571641	2:+13175386	MsaT007048.1:CDS
AGATCCGTGTCCAACGGCCG+AGG	0.574058	2:-13175390	None:intergenic
TACAATTCATGAATATCAAG+GGG	0.575681	2:+13175705	MsaT007048.1:CDS
ACAGTCGTTTAGATTCTCAT+CGG	0.585390	2:+13175311	MsaT007048.1:intron
CTCTTTGATGGTATTCTCGG+AGG	0.601250	2:+13177551	MsaT007048.1:CDS
CGGTGAAGATCCGTGTCCAA+CGG	0.603705	2:-13175396	None:intergenic
CAGATAGTAGCTTGGAACAA+GGG	0.609739	2:-13177261	None:intergenic
TCAGATAGTAGCTTGGAACA+AGG	0.613357	2:-13177262	None:intergenic
GTAGCTTGGAACAAGGGCTG+AGG	0.617170	2:-13177255	None:intergenic
TCCCCATCAACAAGCTCAGG+AGG	0.619846	2:+13178770	MsaT007048.1:CDS
TTCGAAGATATAACCAGGAG+AGG	0.626534	2:+13178719	MsaT007048.1:CDS
GAACTGCATGCACAAAGCAA+CGG	0.628677	2:-13175416	None:intergenic
AGCTGCAAAGAGAAAGGACA+TGG	0.645199	2:+13177202	MsaT007048.1:intron
CTCCGAGAATACCATCAAAG+AGG	0.653875	2:-13177550	None:intergenic
AAAGATTCGAAGATATAACC+AGG	0.674187	2:+13178714	MsaT007048.1:CDS
AGAGAAAGAGTACCTGAAGG+TGG	0.689810	2:-13175751	None:intergenic
AGATGAGTCTAAAAGCACTG+TGG	0.783415	2:+13175341	MsaT007048.1:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	13174915	13178835	13174915	ID=MsaG007048
Chr2	mRNA	13174915	13178835	13174915	ID=MsaT007048.1;Parent=MsaG007048
Chr2	exon	13174915	13174920	13174915	ID=MsaT007048.1.exon1;Parent=MsaT007048.1
Chr2	CDS	13174915	13174920	13174915	ID=cds.MsaT007048.1;Parent=MsaT007048.1
Chr2	exon	13175315	13175459	13175315	ID=MsaT007048.1.exon2;Parent=MsaT007048.1
Chr2	CDS	13175315	13175459	13175315	ID=cds.MsaT007048.1;Parent=MsaT007048.1
Chr2	exon	13175684	13175760	13175684	ID=MsaT007048.1.exon3;Parent=MsaT007048.1
Chr2	CDS	13175684	13175760	13175684	ID=cds.MsaT007048.1;Parent=MsaT007048.1
Chr2	exon	13175948	13175984	13175948	ID=MsaT007048.1.exon4;Parent=MsaT007048.1
Chr2	CDS	13175948	13175984	13175948	ID=cds.MsaT007048.1;Parent=MsaT007048.1
Chr2	exon	13177204	13177300	13177204	ID=MsaT007048.1.exon5;Parent=MsaT007048.1
Chr2	CDS	13177204	13177300	13177204	ID=cds.MsaT007048.1;Parent=MsaT007048.1
Chr2	exon	13177457	13177586	13177457	ID=MsaT007048.1.exon6;Parent=MsaT007048.1
Chr2	CDS	13177457	13177586	13177457	ID=cds.MsaT007048.1;Parent=MsaT007048.1
Chr2	exon	13178695	13178835	13178695	ID=MsaT007048.1.exon7;Parent=MsaT007048.1
Chr2	CDS	13178695	13178835	13178695	ID=cds.MsaT007048.1;Parent=MsaT007048.1

Gene Sequence

>MsaT007048.1

ATGAAGTCGTTTAGATTCTCATCGGACTCAGGAGATGAGTCTAAAAGCACTGTGGTGAGACCTTATGTTCGATCAAAGTTGCCTCGGCCGTTGGACACGGATCTTCACCGTTGCTTTGTGCATGCAGTTCAAAGGCTTGGAGGAGAAGAGAGGGCTACACCAAAGTTAGTTTTACAATTCATGAATATCAAGGGGCTTACCATATCTCATGTCAAAAGCCACCTTCAGATGTACAGGAGCATGAAACAAGAGCAGATGATTGAAGCTGCAAAGAGAAAGGACATGGCAGCTGATTTATCAGTTTCAGCAATCTCCTCCTCAGCCCTTGTTCCAAGCTACTATCTGAATCATGGCCAAAGAATGGATGCTTATTGGCTAGAGTCTTTCAATGGAACTCAAGGTAATCAATCAATTGTTATCTGTAATGGAGATGAGCAGAAGATGCACACTTACATCCTCTTTGATGGTATTCTCGGAGGCCAGACTGTTCAAGGGACCAAAAGCGACCAACAAAGATTCGAAGATATAACCAGGAGAGGAGAGAGAGTGAATGAAGACACAGTTCTGTCCCCATCAACAAGCTCAGGAGGTCCAGACACAAATGATGTCTCTCTTGAGCTCAAACTCAACTGA

Protein sequence

>MsaT007048.1

MKSFRFSSDSGDESKSTVVRPYVRSKLPRPLDTDLHRCFVHAVQRLGGEERATPKLVLQFMNIKGLTISHVKSHLQMYRSMKQEQMIEAAKRKDMAADLSVSAISSSALVPSYYLNHGQRMDAYWLESFNGTQGNQSIVICNGDEQKMHTYILFDGILGGQTVQGTKSDQQRFEDITRRGERVNEDTVLSPSTSSGGPDTNDVSLELKLN*