AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007094


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007094 MtrunA17_Chr2g0289181 44.589 231 33 3 75 210 2 232 8.36e-55 174
MsaG007094 MtrunA17_Chr1g0202461 60.000 60 23 1 64 123 239 297 2.74e-19 84.7
MsaG007094 MtrunA17_Chr7g0248691 49.275 69 35 0 55 123 122 190 8.82e-18 80.1
MsaG007094 MtrunA17_Chr6g0470161 44.318 88 49 0 123 210 32 119 1.12e-17 75.5
MsaG007094 MtrunA17_Chr8g0348311 55.738 61 23 1 63 123 135 191 1.06e-16 77.0
MsaG007094 MtrunA17_Chr2g0284321 50.746 67 29 1 61 123 5 71 3.25e-16 74.3
MsaG007094 MtrunA17_Chr5g0401821 42.308 78 38 1 80 150 192 269 2.85e-15 72.4
MsaG007094 MtrunA17_Chr8g0379461 71.053 38 11 0 88 125 77 114 4.15e-15 71.6
MsaG007094 MtrunA17_Chr2g0319961 44.000 75 33 1 60 125 44 118 7.62e-15 71.2
MsaG007094 MtrunA17_Chr7g0270051 46.970 66 28 1 65 123 24 89 1.30e-14 70.5
MsaG007094 MtrunA17_Chr4g0053281 46.053 76 33 2 80 152 259 329 1.35e-14 71.2
MsaG007094 MtrunA17_Chr1g0184691 66.667 39 13 0 84 122 290 328 1.71e-14 71.2
MsaG007094 MtrunA17_Chr6g0470151 38.843 121 59 4 5 110 233 353 2.01e-14 70.9
MsaG007094 MtrunA17_Chr1g0147881 66.667 39 13 0 84 122 259 297 3.59e-14 70.1
MsaG007094 MtrunA17_Chr7g0228941 46.552 58 26 1 81 138 46 98 4.99e-14 66.2
MsaG007094 MtrunA17_Chr8g0387501 33.871 124 77 2 2 125 49 167 6.54e-14 68.9
MsaG007094 MtrunA17_Chr2g0293481 44.156 77 30 2 60 123 227 303 8.84e-14 69.3
MsaG007094 MtrunA17_Chr3g0139021 40.541 74 38 2 66 138 292 360 1.40e-13 68.6
MsaG007094 MtrunA17_Chr4g0071521 64.103 39 14 0 84 122 242 280 2.77e-13 67.4
MsaG007094 MtrunA17_Chr7g0266611 50.000 88 43 1 123 210 78 164 3.55e-13 65.1
MsaG007094 MtrunA17_Chr5g0447701 47.059 68 32 2 83 150 88 151 2.24e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007094 AT5G52830.1 58.333 60 24 1 64 123 143 201 4.77e-18 81.6
MsaG007094 AT4G01250.1 54.237 59 27 0 65 123 106 164 4.37e-17 78.2
MsaG007094 AT5G45050.4 45.882 85 40 2 54 138 1009 1087 1.89e-16 77.8
MsaG007094 AT5G45050.2 45.882 85 40 2 54 138 1120 1198 2.01e-16 77.8
MsaG007094 AT5G45050.1 45.882 85 40 2 54 138 1148 1226 2.03e-16 77.8
MsaG007094 AT4G23550.1 58.491 53 22 0 71 123 124 176 3.14e-16 75.9
MsaG007094 AT1G29280.1 48.571 70 27 1 63 123 41 110 1.62e-15 73.6
MsaG007094 AT4G31550.1 69.231 39 12 0 84 122 243 281 8.59e-15 72.0
MsaG007094 AT4G31550.2 69.231 39 12 0 84 122 242 280 8.87e-15 72.0
MsaG007094 AT2G24570.1 69.231 39 12 0 84 122 240 278 1.08e-14 71.6
MsaG007094 AT3G58710.2 75.000 36 9 0 88 123 71 106 2.02e-14 70.5
MsaG007094 AT3G58710.1 75.000 36 9 0 88 123 72 107 2.02e-14 70.5
MsaG007094 AT2G23320.1 51.562 64 23 2 88 148 241 299 2.35e-14 70.9
MsaG007094 AT4G24240.1 66.667 39 13 0 84 122 278 316 3.48e-14 70.5
MsaG007094 AT5G28650.1 53.968 63 27 2 84 146 259 319 3.64e-14 70.5
MsaG007094 AT3G04670.1 66.667 39 13 0 84 122 259 297 5.07e-14 70.1
MsaG007094 AT2G30590.1 47.541 61 29 1 65 122 288 348 1.72e-13 68.6
MsaG007094 AT2G34830.2 69.444 36 11 0 88 123 225 260 1.42e-12 66.2
MsaG007094 AT2G34830.1 69.444 36 11 0 88 123 216 251 1.44e-12 66.2
MsaG007094 AT1G30650.1 64.103 39 14 0 85 123 215 253 5.16e-12 64.7
MsaG007094 AT2G38470.1 45.333 75 36 3 71 145 169 238 4.23e-11 62.0

Find 45 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGATGTGGAGACCCTTTAA+TGG 0.287579 2:-13710432 None:intergenic
ATAAATCCAGTTTCAGAATA+AGG 0.301581 2:-13711313 None:intergenic
TATGGACAGAAACCCATTAA+AGG 0.302088 2:+13710419 MsaT007094.1:CDS
TTCACATAACAACAAGATTA+TGG 0.327717 2:+13711234 MsaT007094.1:CDS
AACTTGCCTTATTCTGAAAC+TGG 0.331215 2:+13711307 MsaT007094.1:CDS
TTGGTTAGATGATTACTTAA+TGG 0.339688 2:+13711384 MsaT007094.1:CDS
CACTAAGCAAATCGAGAAAA+AGG 0.340079 2:+13710187 MsaT007094.1:CDS
TCGTGGGCATGGAGGAAATA+TGG 0.346010 2:+13710401 MsaT007094.1:CDS
GAGTGTTAGGAAATTAAAAT+TGG 0.348469 2:-13710046 None:intergenic
GTTGTTGTTCCGCGAGTGTT+AGG 0.379748 2:-13710059 None:intergenic
GGATGTGGAGACCCTTTAAT+GGG 0.385486 2:-13710431 None:intergenic
TCAATGCAAGCAACAGGTTT+GGG 0.387278 2:-13710117 None:intergenic
ATCAATGCAAGCAACAGGTT+TGG 0.388412 2:-13710118 None:intergenic
ATCCACAAATGTTAGAAACT+TGG 0.408052 2:+13711210 MsaT007094.1:CDS
GTGATATCAATGCAAGCAAC+AGG 0.436672 2:-13710123 None:intergenic
GACCCAGCTTCTTCGACAAC+GGG 0.441727 2:-13711187 None:intergenic
ACCCAAGTTTCTAACATTTG+TGG 0.449992 2:-13711212 None:intergenic
ATGGACAGAAACCCATTAAA+GGG 0.461015 2:+13710420 MsaT007094.1:CDS
TATAGCACACCAATGCCGTT+TGG 0.476487 2:-13710352 None:intergenic
TCCACAAATGTTAGAAACTT+GGG 0.479230 2:+13711211 MsaT007094.1:CDS
TTACCCGTTGTCGAAGAAGC+TGG 0.488577 2:+13711184 MsaT007094.1:CDS
GTTAGGAAATTAAAATTGGT+GGG 0.494516 2:-13710042 None:intergenic
AGCAATGCTCAAGTTGTTGT+CGG 0.499521 2:+13711277 MsaT007094.1:CDS
ATGGACTTCAACCGCCTCAA+TGG 0.514519 2:+13711403 MsaT007094.1:CDS
ACATAATAACCAAACGGCAT+TGG 0.525701 2:+13710343 MsaT007094.1:CDS
TGTTAGGAAATTAAAATTGG+TGG 0.530065 2:-13710043 None:intergenic
TGATAACAAACATAACAGCT+TGG 0.533538 2:+13711365 MsaT007094.1:CDS
CAGAAAACATAATAACCAAA+CGG 0.550138 2:+13710337 MsaT007094.1:intron
AGATTGTCCAGCAAGAAGAA+AGG 0.551728 2:+13710930 MsaT007094.1:CDS
TACCCGTTGTCGAAGAAGCT+GGG 0.554513 2:+13711185 MsaT007094.1:CDS
GAATGTGTGAACCTTGGATG+TGG 0.561410 2:-13710446 None:intergenic
TGACCCAGCTTCTTCGACAA+CGG 0.566165 2:-13711188 None:intergenic
TTTCAAAAGACTCGTGGGCA+TGG 0.568404 2:+13710390 MsaT007094.1:CDS
CTATTACAAATGTAGCACTT+TGG 0.573456 2:+13710906 MsaT007094.1:CDS
TTAGGAAATTAAAATTGGTG+GGG 0.575907 2:-13710041 None:intergenic
ATGAACACTAGTGTTTGTCA+TGG 0.581670 2:-13710004 None:intergenic
GACTTCAACCGCCTCAATGG+TGG 0.595091 2:+13711406 MsaT007094.1:CDS
AGAATAGAGCACCACCATTG+AGG 0.605565 2:-13711417 None:intergenic
GATTTGCTTAGTGCAACTGA+TGG 0.610064 2:-13710177 None:intergenic
TTAAAGGGTCTCCACATCCA+AGG 0.615060 2:+13710435 MsaT007094.1:CDS
TGAGCTTTCAAAAGACTCGT+GGG 0.628685 2:+13710385 MsaT007094.1:CDS
ATGAGCTTTCAAAAGACTCG+TGG 0.638619 2:+13710384 MsaT007094.1:CDS
CAAAAGACTCGTGGGCATGG+AGG 0.669284 2:+13710393 MsaT007094.1:CDS
ATAGAGCACCACCATTGAGG+CGG 0.679111 2:-13711414 None:intergenic
ACTTGAGCATTGCTACTCTG+TGG 0.710623 2:-13711268 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 13710006 13711444 13710006 ID=MsaG007094
Chr2 mRNA 13710006 13711444 13710006 ID=MsaT007094.1;Parent=MsaG007094
Chr2 exon 13710006 13710208 13710006 ID=MsaT007094.1.exon1;Parent=MsaT007094.1
Chr2 CDS 13710006 13710208 13710006 ID=cds.MsaT007094.1;Parent=MsaT007094.1
Chr2 exon 13710340 13710456 13710340 ID=MsaT007094.1.exon2;Parent=MsaT007094.1
Chr2 CDS 13710340 13710456 13710340 ID=cds.MsaT007094.1;Parent=MsaT007094.1
Chr2 exon 13710903 13710951 13710903 ID=MsaT007094.1.exon3;Parent=MsaT007094.1
Chr2 CDS 13710903 13710951 13710903 ID=cds.MsaT007094.1;Parent=MsaT007094.1
Chr2 exon 13711178 13711444 13711178 ID=MsaT007094.1.exon4;Parent=MsaT007094.1
Chr2 CDS 13711178 13711444 13711178 ID=cds.MsaT007094.1;Parent=MsaT007094.1
Gene Sequence

>MsaT007094.1

ATGACAAACACTAGTGTTCATGAAATCAATCAATACCCCACCAATTTTAATTTCCTAACACTCGCGGAACAACAACTGATTCAACAAAACATCAACAATCAAGTGTCTGTTCCCAAACCTGTTGCTTGCATTGATATCACAACCGCTCATTTTTATCATGCATACAAACATCCATCAGTTGCACTAAGCAAATCGAGAAAAAGAAAACATAATAACCAAACGGCATTGGTGTGCTATATGAGAGAAGATGAGCTTTCAAAAGACTCGTGGGCATGGAGGAAATATGGACAGAAACCCATTAAAGGGTCTCCACATCCAAGGAGCTATTACAAATGTAGCACTTTGGCAGATTGTCCAGCAAGAAGAAAGAGTCAATTACCCGTTGTCGAAGAAGCTGGGTCATCCACAAATGTTAGAAACTTGGGTTCACATAACAACAAGATTATGGAGTTTGATGAGCCACAGAGTAGCAATGCTCAAGTTGTTGTCGGTGACTCAAACTTGCCTTATTCTGAAACTGGATTTATTAGAAGCTTTGATGATGATGACAATATTTTGATAACAAACATAACAGCTTGGTTAGATGATTACTTAATGGACTTCAACCGCCTCAATGGTGGTGCTCTATTCTCTTAG

Protein sequence

>MsaT007094.1

MTNTSVHEINQYPTNFNFLTLAEQQLIQQNINNQVSVPKPVACIDITTAHFYHAYKHPSVALSKSRKRKHNNQTALVCYMREDELSKDSWAWRKYGQKPIKGSPHPRSYYKCSTLADCPARRKSQLPVVEEAGSSTNVRNLGSHNNKIMEFDEPQSSNAQVVVGDSNLPYSETGFIRSFDDDDNILITNITAWLDDYLMDFNRLNGGALFS*