AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007097

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007097 MtrunA17_Chr2g0289201 97.354 378 6 3 1 378 1 374 0.0 759
MsaG007097 MtrunA17_Chr5g0439391 64.259 263 79 4 121 378 46 298 2.55e-103 306
MsaG007097 MtrunA17_Chr1g0151961 59.684 253 92 4 118 362 116 366 4.84e-90 276
MsaG007097 MtrunA17_Chr5g0413201 53.878 245 111 1 111 353 62 306 6.94e-83 255
MsaG007097 MtrunA17_Chr1g0192791 49.801 251 106 6 116 353 14 257 2.28e-67 214
MsaG007097 MtrunA17_Chr1g0149651 31.200 250 139 5 122 341 174 420 1.91e-37 140
MsaG007097 MtrunA17_Chr2g0305101 30.739 257 149 6 112 341 85 339 2.63e-36 135
MsaG007097 MtrunA17_Chr3g0137241 32.000 250 139 6 122 341 107 355 6.18e-36 134
MsaG007097 MtrunA17_Chr4g0065241 30.576 278 167 6 90 341 38 315 4.75e-35 131
MsaG007097 MtrunA17_Chr5g0398181 28.906 256 141 5 121 341 27 276 3.38e-30 117
MsaG007097 MtrunA17_Chr4g0008591 42.667 75 43 0 269 343 347 421 2.30e-12 68.6
MsaG007097 MtrunA17_Chr3g0141941 39.535 86 46 2 258 343 327 406 5.20e-12 67.0
MsaG007097 MtrunA17_Chr7g0240371 48.333 60 31 0 284 343 307 366 5.93e-12 67.0
MsaG007097 MtrunA17_Chr1g0155681 39.535 86 46 2 258 343 338 417 9.41e-12 66.6
MsaG007097 MtrunA17_Chr8g0373801 39.560 91 50 3 260 347 554 642 1.12e-11 66.6
MsaG007097 MtrunA17_Chr3g0141931 42.254 71 41 0 273 343 339 409 1.78e-11 65.5
MsaG007097 MtrunA17_Chr4g0025521 46.667 60 32 0 284 343 404 463 2.16e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007097 AT1G62360.1 78.707 263 50 5 121 378 121 382 8.67e-132 382
MsaG007097 AT4G08150.1 52.941 306 122 5 71 362 88 385 1.55e-90 278
MsaG007097 AT1G23380.1 51.807 249 114 3 117 362 81 326 4.83e-81 251
MsaG007097 AT1G70510.1 50.410 244 121 0 117 360 65 308 5.70e-80 248
MsaG007097 AT1G23380.2 51.394 251 114 4 117 362 81 328 5.65e-79 246
MsaG007097 AT1G70510.2 49.597 248 121 1 117 360 65 312 9.61e-78 242
MsaG007097 AT4G32040.2 27.701 361 219 6 21 341 4 362 7.74e-43 153
MsaG007097 AT4G32040.1 27.701 361 219 6 21 341 4 362 7.83e-43 154
MsaG007097 AT5G25220.2 32.653 245 139 5 122 341 160 403 9.86e-41 149
MsaG007097 AT5G25220.1 32.653 245 139 5 122 341 160 403 1.07e-40 149
MsaG007097 AT5G11060.1 31.429 245 142 6 122 341 124 367 2.27e-36 136
MsaG007097 AT1G62990.1 30.924 249 142 6 121 341 29 275 2.20e-31 120
MsaG007097 AT1G75430.1 49.091 55 28 0 289 343 209 263 2.24e-11 64.3
MsaG007097 AT5G02030.1 46.667 60 32 0 284 343 350 409 2.93e-11 65.5
MsaG007097 AT1G19700.1 46.667 60 32 0 284 343 353 412 4.19e-11 64.7
MsaG007097 AT1G19700.3 46.667 60 32 0 284 343 353 412 4.19e-11 64.7
MsaG007097 AT1G19700.5 46.667 60 32 0 284 343 353 412 4.19e-11 64.7
MsaG007097 AT1G19700.4 46.667 60 32 0 284 343 353 412 4.19e-11 64.7
MsaG007097 AT1G19700.2 46.667 60 32 0 284 343 353 412 4.19e-11 64.7
MsaG007097 AT4G36870.2 39.024 82 46 2 264 343 480 559 7.63e-11 64.3
MsaG007097 AT4G36870.1 39.024 82 46 2 264 343 480 559 7.63e-11 64.3
MsaG007097 AT2G27220.1 45.000 60 33 0 284 343 230 289 9.44e-11 63.5
MsaG007097 AT2G27220.3 45.000 60 33 0 284 343 230 289 9.44e-11 63.5

Find 83 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTATTATTGTTAGTGGAATT+AGG 0.210507 2:+13748376 None:intergenic
AGAACTTTCCAAACCCTTAA+AGG 0.230018 2:-13747713 MsaT007097.1:CDS
ATTAGTATTGTTGTGGTTAT+TGG 0.247015 2:+13748432 None:intergenic
TCTTATGTGATGGGTGCTTT+TGG 0.272814 2:-13748541 MsaT007097.1:CDS
AGCAGAAGACAGGGAATTAA+AGG 0.275275 2:-13744141 MsaT007097.1:CDS
CATAGCTTCCTTTAAGGGTT+TGG 0.275443 2:+13747705 None:intergenic
AATTGTTGCCTTGCCTCTTT+TGG 0.282916 2:+13744038 None:intergenic
TTGTGGTTATTGGAATTTAT+TGG 0.285636 2:+13748442 None:intergenic
TGGTTTAGGTTACTATTTCA+TGG 0.291622 2:-13748284 MsaT007097.1:CDS
CATCAAAACACTCCTGGTTT+AGG 0.312805 2:-13748298 MsaT007097.1:CDS
AAAAGCATAGCTTCCTTTAA+GGG 0.321776 2:+13747700 None:intergenic
TCTTCTTCTTCAGATAATAT+TGG 0.323037 2:-13747637 MsaT007097.1:intron
TATTATTGTTAGTGGAATTA+GGG 0.324374 2:+13748377 None:intergenic
TTCTTCTTATTCTTATGTGA+TGG 0.373753 2:-13748551 MsaT007097.1:CDS
GCAGTTGGATCAGGTTGTTT+AGG 0.376419 2:-13747799 MsaT007097.1:CDS
GTGATTGTTATTATTGTTAG+TGG 0.377361 2:+13748369 None:intergenic
TCAATGGAGGGTAGTTCTAA+TGG 0.389732 2:-13748577 MsaT007097.1:CDS
ATCAGAAGCAAATAAACAAC+TGG 0.411205 2:-13743153 MsaT007097.1:CDS
TCTTCTTATTCTTATGTGAT+GGG 0.426756 2:-13748550 MsaT007097.1:CDS
CAAGGCAACAATTGCTTGAA+TGG 0.428670 2:-13744028 MsaT007097.1:CDS
AACCTTGCCACCACTTCACT+TGG 0.434594 2:+13747862 None:intergenic
TCCAGCTCTAGATCAGTTCA+TGG 0.439689 2:-13747770 MsaT007097.1:CDS
GTGAGGATGAGCCATGATCT+TGG 0.442354 2:+13748207 None:intergenic
AATTCCCTGTCTTCTGCTTG+AGG 0.446914 2:+13744146 None:intergenic
AGAGGTGTTGTTATTACAGT+TGG 0.455218 2:+13748342 None:intergenic
AGCAAGAGTTTATGAAGAAA+AGG 0.455849 2:-13744076 MsaT007097.1:CDS
AGAAAATACAGTGGATACTT+AGG 0.456169 2:-13744107 MsaT007097.1:CDS
AAGAGGAAGAAGAGCTTCCA+TGG 0.462925 2:+13748248 None:intergenic
ACTAAAGGCATCATCATCAT+TGG 0.476116 2:+13748493 None:intergenic
TCCTCACTATCACCGTCTCT+TGG 0.481181 2:-13748191 MsaT007097.1:CDS
TTCAGAGGACATGCAGTTTG+TGG 0.481599 2:-13743098 MsaT007097.1:CDS
TAGATCCTCAAGCAGAAGAC+AGG 0.488640 2:-13744151 MsaT007097.1:CDS
TTCTGTCAAATCCAAGATCA+TGG 0.491565 2:-13748218 MsaT007097.1:CDS
CTTGTAGTGAAGGTGGAGAT+AGG 0.494451 2:-13744220 MsaT007097.1:CDS
TCAATCAAAGGAAACGGCAC+TGG 0.497928 2:-13743126 MsaT007097.1:CDS
CTGGCTCTAGCAGAGTCAAC+AGG 0.498247 2:-13743181 MsaT007097.1:CDS
ATGTGCAACTGCAGTGAGAA+TGG 0.501403 2:-13747830 MsaT007097.1:CDS
ATTGTTAGTGGAATTAGGGA+TGG 0.507470 2:+13748381 None:intergenic
GGTGGAGATGCAGTTGGATC+AGG 0.509504 2:-13747808 MsaT007097.1:CDS
TTTGACAGAAGAAGAAGAAG+AGG 0.516596 2:+13748231 None:intergenic
GGACATGCAGTTTGTGGTGA+TGG 0.522795 2:-13743092 MsaT007097.1:CDS
GCAGAAGACAGGGAATTAAA+GGG 0.524288 2:-13744140 MsaT007097.1:CDS
AGCCTACATCAACTGTCAAA+AGG 0.527463 2:-13748167 MsaT007097.1:intron
AAAAGGAAAGCTTCCAAAAG+AGG 0.530019 2:-13744051 MsaT007097.1:CDS
TAGTGGAATTAGGGATGGGA+AGG 0.531021 2:+13748386 None:intergenic
GGTTTATCAATCAAAGGAAA+CGG 0.544135 2:-13743132 MsaT007097.1:CDS
AGTGAAGGTGGAGATAGGAA+TGG 0.544711 2:-13744215 MsaT007097.1:CDS
ATAACAGGAATCACAGAAGC+TGG 0.544820 2:-13743200 MsaT007097.1:intron
AATAACCATCAAAACACTCC+TGG 0.547863 2:-13748304 MsaT007097.1:CDS
TCCATGAACTGATCTAGAGC+TGG 0.552608 2:+13747769 None:intergenic
TTGTTAGTGGAATTAGGGAT+GGG 0.555961 2:+13748382 None:intergenic
ATGTAATAGTGTGGATGACT+TGG 0.557999 2:+13743067 None:intergenic
TGTTTGCATTAGTATTGTTG+TGG 0.559213 2:+13748425 None:intergenic
ACAACTGGTTTATCAATCAA+AGG 0.560044 2:-13743138 MsaT007097.1:CDS
CACCAAGTGAAGTGGTGGCA+AGG 0.566973 2:-13747864 MsaT007097.1:CDS
TGATGAGATGAAGTGACTAA+AGG 0.572274 2:+13748478 None:intergenic
ATATGATCAGCTTGTAGTGA+AGG 0.580233 2:-13744230 MsaT007097.1:intron
AAAGGCATCATCATCATTGG+AGG 0.581233 2:+13748496 None:intergenic
AAGTCATCCACACTATTACA+TGG 0.581325 2:-13743065 MsaT007097.1:CDS
CATGGTGTTAGATAGATCCA+TGG 0.582210 2:+13743018 None:intergenic
TGTGCAACTGCAGTGAGAAT+GGG 0.589860 2:-13747829 MsaT007097.1:CDS
GGTTGGAGCACCAAGTGAAG+TGG 0.591898 2:-13747872 MsaT007097.1:CDS
TCTCAGCAATTCATATCCCA+TGG 0.592615 2:-13743035 MsaT007097.1:CDS
ACAAACTGCATGTCCTCTGA+AGG 0.595663 2:+13743100 None:intergenic
TGATCAGCTTGTAGTGAAGG+TGG 0.598391 2:-13744227 MsaT007097.1:intron
GTGAGTATTAATTCAATGGA+GGG 0.604163 2:-13748589 None:intergenic
GGCAACAATTGCTTGAATGG+TGG 0.608396 2:-13744025 MsaT007097.1:CDS
TCCAAGAGACGGTGATAGTG+AGG 0.612274 2:+13748190 None:intergenic
TGGAGCACCAAGTGAAGTGG+TGG 0.614555 2:-13747869 MsaT007097.1:CDS
ACTAATGCAAACAACATAAG+CGG 0.624162 2:-13748415 MsaT007097.1:CDS
GAAAGCTTCCAAAAGAGGCA+AGG 0.624760 2:-13744046 MsaT007097.1:CDS
TGATGTAGGCTTCCAAGAGA+CGG 0.625494 2:+13748179 None:intergenic
ACATTATCCATGTAATAGTG+TGG 0.627160 2:+13743058 None:intergenic
ATGGACAACATCAACAACCA+TGG 0.630008 2:-13748265 MsaT007097.1:CDS
ACGGCACTGGAAGCCTTCAG+AGG 0.633606 2:-13743113 MsaT007097.1:CDS
GTGGTGCTTACAGATGGGTA+AGG 0.637343 2:+13743984 None:intergenic
ATGGTGTTAGATAGATCCAT+GGG 0.639715 2:+13743019 None:intergenic
AGATCCTCAAGCAGAAGACA+GGG 0.640900 2:-13744150 MsaT007097.1:CDS
ATGAAATAGTAACCTAAACC+AGG 0.642596 2:+13748286 None:intergenic
CCGAGGTAATAACTAGAGCA+TGG 0.644204 2:+13743000 None:intergenic
AGAATGGGTGGAGATGCAGT+TGG 0.655702 2:-13747814 MsaT007097.1:CDS
GCAACTGCAGTGAGAATGGG+TGG 0.659150 2:-13747826 MsaT007097.1:CDS
GGTGGAGCAGACATTACAAG+TGG 0.680780 2:-13744007 MsaT007097.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 13743012 13748596 13743012 ID=MsaG007097
Chr2 mRNA 13743012 13748596 13743012 ID=MsaT007097.1;Parent=MsaG007097
Chr2 exon 13743012 13743215 13743012 ID=MsaT007097.1.exon4;Parent=MsaT007097.1
Chr2 CDS 13743012 13743215 13743012 ID=cds.MsaT007097.1;Parent=MsaT007097.1
Chr2 exon 13743995 13744242 13743995 ID=MsaT007097.1.exon3;Parent=MsaT007097.1
Chr2 CDS 13743995 13744242 13743995 ID=cds.MsaT007097.1;Parent=MsaT007097.1
Chr2 exon 13747638 13747893 13747638 ID=MsaT007097.1.exon2;Parent=MsaT007097.1
Chr2 CDS 13747638 13747893 13747638 ID=cds.MsaT007097.1;Parent=MsaT007097.1
Chr2 exon 13748168 13748596 13748168 ID=MsaT007097.1.exon1;Parent=MsaT007097.1
Chr2 CDS 13748168 13748596 13748168 ID=cds.MsaT007097.1;Parent=MsaT007097.1
Gene Sequence

>MsaT007097.1

ATGGAGGGTAGTTCTAATGGAAGTTCTTCTTATTCTTATGTGATGGGTGCTTTTGGAGAAAACAGTGGTGGGCTATGTCCTCCAATGATGATGATGCCTTTAGTCACTTCATCTCATCATAATGCTCATCATCCAATAAATTCCAATAACCACAACAATACTAATGCAAACAACATAAGCGGTCTTTTCCTTCCCATCCCTAATTCCACTAACAATAATAACAATCACTACACCAACTGTAATAACAACACCTCTTCTATCATGCTTCAAAATAACCATCAAAACACTCCTGGTTTAGGTTACTATTTCATGGACAACATCAACAACCATGGAAGCTCTTCTTCCTCTTCTTCTTCTTCTGTCAAATCCAAGATCATGGCTCATCCTCACTATCACCGTCTCTTGGAAGCCTACATCAACTGTCAAAAGGTTGGAGCACCAAGTGAAGTGGTGGCAAGGTTAGAAGAAGCATGTGCAACTGCAGTGAGAATGGGTGGAGATGCAGTTGGATCAGGTTGTTTAGGTGAAGATCCAGCTCTAGATCAGTTCATGGAAGCTTACTGTGAGATGCTTATCAAGTATGAGCAAGAACTTTCCAAACCCTTAAAGGAAGCTATGCTTTTTCTTCAAAGGATTGAAGTCCAGTTCAAAAATCTTACAGTTTCTTCTTCTTCAGATAATATTGCTTGTAGTGAAGGTGGAGATAGGAATGGATCATCTGAAGAAGATCATGTTGATATGTACGACAACATGATAGATCCTCAAGCAGAAGACAGGGAATTAAAGGGTCAGCTTTTGAGAAAATACAGTGGATACTTAGGCAGTCTGAAGCAAGAGTTTATGAAGAAAAGGAAAAAAGGAAAGCTTCCAAAAGAGGCAAGGCAACAATTGCTTGAATGGTGGAGCAGACATTACAAGTGGCCTTACCCATCTGAATCACAGAAGCTGGCTCTAGCAGAGTCAACAGGTCTTGATCAGAAGCAAATAAACAACTGGTTTATCAATCAAAGGAAACGGCACTGGAAGCCTTCAGAGGACATGCAGTTTGTGGTGATGGATCCAAGTCATCCACACTATTACATGGATAATGTTCTCAGCAATTCATATCCCATGGATCTATCTAACACCATGCTCTAG
Protein sequence

>MsaT007097.1

MEGSSNGSSSYSYVMGAFGENSGGLCPPMMMMPLVTSSHHNAHHPINSNNHNNTNANNISGLFLPIPNSTNNNNNHYTNCNNNTSSIMLQNNHQNTPGLGYYFMDNINNHGSSSSSSSSSVKSKIMAHPHYHRLLEAYINCQKVGAPSEVVARLEEACATAVRMGGDAVGSGCLGEDPALDQFMEAYCEMLIKYEQELSKPLKEAMLFLQRIEVQFKNLTVSSSSDNIACSEGGDRNGSSEEDHVDMYDNMIDPQAEDRELKGQLLRKYSGYLGSLKQEFMKKRKKGKLPKEARQQLLEWWSRHYKWPYPSESQKLALAESTGLDQKQINNWFINQRKRHWKPSEDMQFVVMDPSHPHYYMDNVLSNSYPMDLSNTML*