AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007101

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007101 MtrunA17_Chr2g0289221 91.900 321 25 1 1 320 1 321 0.0 618
MsaG007101 MtrunA17_Chr5g0417281 59.339 514 191 8 13 511 4 514 0.0 563
MsaG007101 MtrunA17_Chr5g0436761 54.167 504 179 7 9 506 1 458 5.27e-178 508
MsaG007101 MtrunA17_Chr5g0417211 53.150 508 130 9 9 511 1 405 1.39e-165 474
MsaG007101 MtrunA17_Chr5g0424931 50.369 542 211 12 7 511 6 526 1.59e-165 479
MsaG007101 MtrunA17_Chr1g0178011 45.294 510 197 14 9 504 1 442 7.66e-131 388
MsaG007101 MtrunA17_Chr2g0277461 45.365 507 206 11 13 510 6 450 1.04e-127 380
MsaG007101 MtrunA17_Chr3g0115271 43.393 560 248 13 10 511 9 557 8.98e-126 379
MsaG007101 MtrunA17_Chr5g0425261 49.462 372 153 14 9 361 1 356 1.22e-99 305
MsaG007101 MtrunA17_Chr5g0424741 40.713 533 223 18 9 464 1 517 3.39e-99 309
MsaG007101 MtrunA17_Chr5g0424801 50.427 351 140 13 9 340 1 336 1.12e-98 302
MsaG007101 MtrunA17_Chr2g0277531 48.408 314 146 10 14 320 14 318 1.78e-88 274
MsaG007101 MtrunA17_Chr2g0326761 40.148 406 168 11 17 361 8 399 1.21e-86 281
MsaG007101 MtrunA17_Chr1g0181211 40.148 406 174 10 17 360 24 422 7.45e-85 276
MsaG007101 MtrunA17_Chr4g0028971 39.320 412 179 10 9 358 1 403 1.37e-83 273
MsaG007101 MtrunA17_Chr1g0199681 35.547 512 244 15 17 464 91 580 7.28e-82 268
MsaG007101 MtrunA17_Chr7g0264171 37.379 412 184 12 17 362 28 431 3.88e-76 251
MsaG007101 MtrunA17_Chr5g0425661 53.571 196 85 2 20 213 37 228 3.07e-64 209
MsaG007101 MtrunA17_Chr7g0264161 40.635 315 148 10 30 320 2 301 5.38e-64 210
MsaG007101 MtrunA17_Chr2g0282961 35.176 398 221 11 11 382 45 431 3.18e-59 206
MsaG007101 MtrunA17_Chr4g0046371 31.947 457 255 13 20 446 38 468 1.16e-55 198
MsaG007101 MtrunA17_Chr2g0276781 35.977 353 194 12 20 350 23 365 6.13e-52 187
MsaG007101 MtrunA17_Chr8g0385791 34.688 369 215 10 20 368 59 421 3.87e-51 187
MsaG007101 MtrunA17_Chr4g0009751 33.148 359 212 10 20 358 19 369 3.83e-50 181
MsaG007101 MtrunA17_Chr2g0285461 33.060 366 201 12 11 345 11 363 9.97e-50 183
MsaG007101 MtrunA17_Chr8g0346711 35.000 360 202 12 20 358 29 377 2.63e-49 180
MsaG007101 MtrunA17_Chr2g0326281 32.727 385 201 13 20 358 56 428 8.13e-49 180
MsaG007101 MtrunA17_Chr7g0239061 33.516 364 203 13 20 358 14 363 2.26e-48 177
MsaG007101 MtrunA17_Chr2g0301251 31.549 355 196 11 17 341 20 357 7.15e-48 178
MsaG007101 MtrunA17_Chr5g0433301 32.670 352 200 10 20 345 24 364 1.72e-47 176
MsaG007101 MtrunA17_Chr4g0029671 32.713 376 225 8 20 369 54 427 2.19e-47 176
MsaG007101 MtrunA17_Chr4g0070181 31.728 353 194 11 17 339 20 355 4.81e-47 176
MsaG007101 MtrunA17_Chr3g0109391 32.386 352 201 10 20 345 26 366 2.44e-46 173
MsaG007101 MtrunA17_Chr8g0376771 33.152 368 197 15 11 345 11 362 3.01e-46 173
MsaG007101 MtrunA17_Chr1g0156321 35.244 349 182 15 20 339 43 376 5.67e-46 172
MsaG007101 MtrunA17_Chr8g0389081 31.549 355 200 12 17 342 29 369 4.07e-44 167
MsaG007101 MtrunA17_Chr5g0424791 44.000 150 72 6 71 219 13 151 1.01e-23 97.4
MsaG007101 MtrunA17_Chr5g0425651 49.558 113 49 6 238 343 1 112 2.65e-23 95.1
MsaG007101 MtrunA17_Chr8g0356211 27.354 223 145 5 130 339 2 220 6.24e-23 99.4
MsaG007101 MtrunA17_Chr5g0419861 42.623 122 64 2 52 170 5 123 1.13e-18 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007101 AT1G77850.1 37.017 543 268 15 9 489 1 531 4.59e-100 313
MsaG007101 AT1G77850.2 37.017 543 268 15 9 489 1 531 5.53e-100 313
MsaG007101 AT4G30080.1 40.141 426 174 14 17 379 16 423 9.25e-83 270
MsaG007101 AT2G28350.2 38.702 416 178 12 13 363 3 406 9.92e-80 262
MsaG007101 AT2G28350.1 38.702 416 178 12 13 363 3 406 1.81e-79 262
MsaG007101 AT2G46530.1 36.240 367 191 16 20 358 21 372 7.10e-55 194
MsaG007101 AT2G46530.3 36.240 367 191 16 20 358 42 393 2.13e-54 193
MsaG007101 AT3G61830.1 36.176 340 187 13 20 339 25 354 1.02e-53 191
MsaG007101 AT1G35520.1 36.170 376 207 14 3 358 10 372 1.87e-52 187
MsaG007101 AT5G37020.2 34.091 352 195 10 20 345 22 362 1.04e-51 188
MsaG007101 AT5G37020.1 34.091 352 195 10 20 345 22 362 1.66e-51 188
MsaG007101 AT4G23980.2 33.923 339 197 9 20 339 12 342 1.83e-51 186
MsaG007101 AT4G23980.1 33.923 339 197 9 20 339 12 342 1.88e-51 186
MsaG007101 AT1G34390.1 35.638 376 207 15 3 358 10 370 3.58e-51 184
MsaG007101 AT2G33860.1 35.072 345 189 10 20 339 54 388 5.74e-51 184
MsaG007101 AT1G34310.1 35.263 380 205 14 3 358 10 372 7.15e-51 183
MsaG007101 AT1G34410.1 34.574 376 213 13 3 358 10 372 7.77e-51 183
MsaG007101 AT5G62000.2 33.983 359 211 10 20 358 61 413 1.95e-49 182
MsaG007101 AT5G62000.5 33.983 359 211 10 20 358 61 413 1.95e-49 182
MsaG007101 AT5G62000.3 33.983 359 211 10 20 358 61 413 1.95e-49 182
MsaG007101 AT5G62000.1 33.983 359 211 10 20 358 61 413 1.95e-49 182
MsaG007101 AT5G62000.4 33.983 359 211 10 20 358 61 413 2.25e-49 182
MsaG007101 AT1G35540.1 34.181 354 205 12 3 339 10 352 2.30e-49 179
MsaG007101 AT1G59750.2 34.503 342 192 11 20 339 22 353 3.24e-49 180
MsaG007101 AT1G59750.4 34.503 342 192 11 20 339 22 353 3.29e-49 180
MsaG007101 AT1G59750.1 34.503 342 192 11 20 339 22 353 4.05e-49 179
MsaG007101 AT1G59750.3 34.503 342 192 11 20 339 22 353 4.05e-49 179
MsaG007101 AT5G60450.2 34.865 370 207 13 15 356 36 399 1.45e-48 179
MsaG007101 AT1G35240.1 34.492 374 216 13 3 358 10 372 2.04e-48 176
MsaG007101 AT1G30330.1 32.877 365 203 11 12 345 10 363 2.57e-48 179
MsaG007101 AT5G60450.1 35.068 365 203 13 20 356 65 423 2.99e-48 178
MsaG007101 AT1G30330.3 32.698 367 203 11 12 345 10 365 7.41e-48 178
MsaG007101 AT1G30330.2 32.698 367 203 11 12 345 10 365 7.41e-48 178
MsaG007101 AT1G19850.2 34.540 359 178 12 17 339 40 377 1.68e-46 174
MsaG007101 AT1G19850.1 34.540 359 178 12 17 339 51 388 1.74e-46 174
MsaG007101 AT5G20730.3 31.161 353 197 10 17 339 21 357 1.62e-44 169
MsaG007101 AT5G20730.1 31.161 353 197 10 17 339 21 357 1.80e-44 169
MsaG007101 AT1G19220.1 31.549 355 196 12 17 341 20 357 2.10e-44 168
MsaG007101 AT5G20730.2 31.161 353 196 11 17 339 21 356 2.90e-43 165
MsaG007101 AT1G34170.1 31.234 381 210 14 20 369 25 384 1.24e-42 159
MsaG007101 AT3G61830.2 36.842 285 154 11 72 339 28 303 4.15e-42 158
MsaG007101 AT2G46530.2 37.638 271 142 11 106 358 24 285 5.53e-40 152
MsaG007101 AT2G33860.2 37.931 232 124 7 124 339 4 231 8.44e-40 150
MsaG007101 AT5G37020.3 35.685 241 130 8 124 345 33 267 1.77e-37 147
MsaG007101 AT1G34170.2 30.376 372 199 14 20 369 25 358 1.41e-36 141
MsaG007101 AT1G34170.3 30.376 372 199 14 20 369 25 358 2.92e-36 142
MsaG007101 AT1G43950.1 36.957 230 120 7 2 222 9 222 4.61e-32 123
MsaG007101 ATMG00940.1 38.679 106 60 3 211 316 44 144 3.34e-13 68.2
MsaG007101 AT4G01500.1 30.597 134 90 1 124 257 38 168 2.86e-11 65.5

Find 137 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGGTTCATTTATGCTATT+TGG 0.105998 2:+13787764 MsaT007101.1:CDS
AACATTAGCACATCTATATT+TGG 0.209645 2:+13787653 MsaT007101.1:CDS
AATATATATTATAGGTTAAC+TGG 0.211032 2:+13787273 MsaT007101.1:intron
GGAGACATGGCGGAAATATT+TGG 0.255210 2:-13785354 None:intergenic
CTTCCAACTCTTTCAATGAT+AGG 0.271660 2:+13787570 MsaT007101.1:CDS
TTCTTTGGTGCTGCACTTGT+GGG 0.284414 2:-13787735 None:intergenic
TGAACCAGGATTAACTTCTT+TGG 0.304162 2:-13787750 None:intergenic
AGGGATAGGGTTTCAGTGTT+GGG 0.310433 2:-13785507 None:intergenic
ACCTCCCAAGGGCTTTGCTT+TGG 0.312781 2:-13787320 None:intergenic
GACACCTGATCACCACTAAT+TGG 0.319561 2:+13785895 MsaT007101.1:CDS
GCGCAGCACCGCCAAATCTT+TGG 0.327738 2:-13785399 None:intergenic
CCGACGTTCATGGCAAAGTC+TGG 0.342190 2:+13785838 MsaT007101.1:CDS
TACAATTGCCCTCAGCAAAA+AGG 0.346652 2:+13787375 MsaT007101.1:CDS
TTTGGATTATGTGAGACTTC+AGG 0.376866 2:-13787302 None:intergenic
ATCCAACAATGTTTGATTCA+AGG 0.377439 2:-13787486 None:intergenic
AGGATGCAATGAAGATTAAC+TGG 0.377747 2:+13786159 MsaT007101.1:CDS
AATCTTTGGTTCTACACGGC+TGG 0.380640 2:-13785385 None:intergenic
ATCCGTCGGCATACTAAGTT+TGG 0.389012 2:+13786005 MsaT007101.1:CDS
ATCCTTGAATCAAACATTGT+TGG 0.389726 2:+13787484 MsaT007101.1:CDS
ACCCTATCTGATGCTAATAA+TGG 0.393308 2:+13785723 MsaT007101.1:CDS
CTTCTTTGGTGCTGCACTTG+TGG 0.393725 2:-13787736 None:intergenic
CCCTTTGAGACAAAGTTTGA+CGG 0.397840 2:+13787710 MsaT007101.1:CDS
CACCATTATTAGCATCAGAT+AGG 0.401174 2:-13785725 None:intergenic
AATATAGATGTGCTAATGTT+TGG 0.410256 2:-13787650 None:intergenic
CTGTCTGTTACCGACGTTCA+TGG 0.414559 2:+13785828 MsaT007101.1:CDS
CCGTCAAACTTTGTCTCAAA+GGG 0.416058 2:-13787710 None:intergenic
GGTGATGAAATTGTTTCCTC+TGG 0.421466 2:+13785690 MsaT007101.1:CDS
GGGATAGGGTTTCAGTGTTG+GGG 0.425954 2:-13785506 None:intergenic
GCCGTGTAGAACCAAAGATT+TGG 0.431938 2:+13785388 MsaT007101.1:CDS
TACTCCAATTAGTGGTGATC+AGG 0.433415 2:-13785899 None:intergenic
AGCATGCGCCATGGACGATT+AGG 0.434996 2:-13786275 None:intergenic
TCATCTGGAGATATGTTTGT+TGG 0.440275 2:+13785981 MsaT007101.1:CDS
GAAACCCTATCCCTTCTTGA+TGG 0.440854 2:+13785516 MsaT007101.1:CDS
AACCATCAGTGATGGGAGTT+AGG 0.442469 2:-13785623 None:intergenic
CATGTTCTAAGTGACCTTGC+GGG 0.450390 2:-13785470 None:intergenic
CAAGGGAGAAGATGGTTGTA+TGG 0.451305 2:+13787832 MsaT007101.1:CDS
ATCTTTGGTTCTACACGGCT+GGG 0.452340 2:-13785384 None:intergenic
CACGAACTTACTCCAATTAG+TGG 0.453246 2:-13785907 None:intergenic
GGTTATGGCGGCAGCGGAGT+TGG 0.455795 2:+13786052 MsaT007101.1:CDS
GAGATGGCTGTTTAGTTTCG+AGG 0.456957 2:-13785800 None:intergenic
AAGGGATAGGGTTTCAGTGT+TGG 0.458344 2:-13785508 None:intergenic
TCATGCAAATTACTTCCAAC+AGG 0.463742 2:-13787800 None:intergenic
TTCAAAATGCTATGGTTAAG+AGG 0.466292 2:-13787686 None:intergenic
GATAACTAAAGAATCGGTTA+TGG 0.468357 2:+13786037 MsaT007101.1:CDS
GGTTCTACACGGCTGGGTTG+TGG 0.468361 2:-13785378 None:intergenic
TTTCAATCCTTGCATTGTAT+CGG 0.468436 2:+13785548 MsaT007101.1:CDS
GAGTATTATCCTACGATTGA+AGG 0.470883 2:+13786101 MsaT007101.1:CDS
TTCCAACTCTTTCAATGATA+GGG 0.473757 2:+13787571 MsaT007101.1:CDS
ACAAAGACTTCATCAGTATG+AGG 0.479542 2:-13785591 None:intergenic
AAGATTTGGCGGTGCTGCGC+CGG 0.479939 2:+13785402 MsaT007101.1:CDS
GATAGGGTTTCAGTGTTGGG+GGG 0.483168 2:-13785504 None:intergenic
ACCGTCAAACTTTGTCTCAA+AGG 0.497762 2:-13787711 None:intergenic
CATTGCATCCTCAACAACCT+TGG 0.498204 2:-13786147 None:intergenic
GGATAGGGTTTCAGTGTTGG+GGG 0.498312 2:-13785505 None:intergenic
AGAATCGGTTATGGCGGCAG+CGG 0.500580 2:+13786046 MsaT007101.1:CDS
GGCCCTATCATTGAAAGAGT+TGG 0.504567 2:-13787573 None:intergenic
CTGGAAATCTATCATTGGAA+TGG 0.511209 2:-13787447 None:intergenic
TCTGCTACCAAGGGAGAAGA+TGG 0.513250 2:+13787824 MsaT007101.1:CDS
GAATGCTGGAAATCTATCAT+TGG 0.515653 2:-13787452 None:intergenic
GAAATATTTGGTTCTACCAT+CGG 0.518665 2:-13785342 None:intergenic
TGCTAAGATAACTAAAGAAT+CGG 0.520496 2:+13786031 MsaT007101.1:CDS
CGTGACCATCAAGAAGGGAT+AGG 0.523750 2:-13785521 None:intergenic
TCTCTCTATATTATCTAATG+AGG 0.525248 2:-13787420 None:intergenic
TTTGCATGATTCTGCTACCA+AGG 0.526896 2:+13787814 MsaT007101.1:CDS
GCTGGGTTGTGGTGGAGACA+TGG 0.530128 2:-13785367 None:intergenic
ATATTATAGGTTAACTGGGA+TGG 0.530339 2:+13787278 MsaT007101.1:intron
AGTGGTGATCAGGTGTCTCA+AGG 0.534349 2:-13785889 None:intergenic
TAATCCAAAGCAAAGCCCTT+GGG 0.536094 2:+13787316 MsaT007101.1:CDS
GTATGGGCTGCAATAATTGA+AGG 0.536513 2:+13787849 MsaT007101.1:CDS
GAAATTAGTTCAACCTCCCA+AGG 0.540471 2:-13787332 None:intergenic
TATGGCGGCAGCGGAGTTGG+CGG 0.543054 2:+13786055 MsaT007101.1:CDS
ATGAAGATTAACTGGAATTG+TGG 0.543117 2:+13786167 MsaT007101.1:CDS
CACTAATTGGAGTAAGTTCG+TGG 0.543836 2:+13785908 MsaT007101.1:CDS
GATACAATGCAAGGATTGAA+AGG 0.544112 2:-13785546 None:intergenic
CAAAGGGGTTCAAAATGCTA+TGG 0.546020 2:-13787694 None:intergenic
CTCCTAACTCCCATCACTGA+TGG 0.547018 2:+13785621 MsaT007101.1:CDS
ATAATCCAAAGCAAAGCCCT+TGG 0.548641 2:+13787315 MsaT007101.1:CDS
ACCATTATTAGCATCAGATA+GGG 0.549720 2:-13785724 None:intergenic
CATACAACCATCTTCTCCCT+TGG 0.552659 2:-13787831 None:intergenic
TCGTCCATGGCGCATGCTTG+AGG 0.553392 2:+13786280 MsaT007101.1:CDS
TCTCTGCTCCTAATCGTCCA+TGG 0.557073 2:+13786267 MsaT007101.1:CDS
TTGCATGATTCTGCTACCAA+GGG 0.558661 2:+13787815 MsaT007101.1:CDS
TCTTTGGTGCTGCACTTGTG+GGG 0.558880 2:-13787734 None:intergenic
ATGATGATTATTCAAAGAGA+TGG 0.559089 2:+13786216 MsaT007101.1:CDS
GTGTAGAACCAAAGATTTGG+CGG 0.559765 2:+13785391 MsaT007101.1:CDS
GGCAATTGTAGATTCAGTGC+AGG 0.560065 2:-13787362 None:intergenic
TCCTACTGAGGTTCGTGAAG+AGG 0.565181 2:+13785659 MsaT007101.1:CDS
AGTATTATCCTACGATTGAA+GGG 0.565286 2:+13786102 MsaT007101.1:CDS
AAGGGAGAAGATGGTTGTAT+GGG 0.567406 2:+13787833 MsaT007101.1:CDS
GGTGGATGCCAAGGTTGTTG+AGG 0.569678 2:+13786139 MsaT007101.1:CDS
TCACAAAACCCTTCAATCGT+AGG 0.570407 2:-13786110 None:intergenic
CGACGTTCATGGCAAAGTCT+GGG 0.573902 2:+13785839 MsaT007101.1:CDS
TGTTCACGAACCTCCTACTG+AGG 0.580009 2:+13785647 MsaT007101.1:CDS
GTGAACACAACCATCAGTGA+TGG 0.580232 2:-13785631 None:intergenic
CCAAATAGCATAAATGAACC+AGG 0.581265 2:-13787764 None:intergenic
AGCACCAAAGAAGTTAATCC+TGG 0.583231 2:+13787746 MsaT007101.1:CDS
TCTTCACGAACCTCAGTAGG+AGG 0.584861 2:-13785657 None:intergenic
AAAGTTTACTATTTCCCGCA+AGG 0.585623 2:+13785456 MsaT007101.1:CDS
GTGACCATCAAGAAGGGATA+GGG 0.586087 2:-13785520 None:intergenic
ATATGTTTGTTGGAATCCGT+CGG 0.586941 2:+13785991 MsaT007101.1:CDS
GTTCGTGAAGAGGAACCTAC+AGG 0.587140 2:+13785669 MsaT007101.1:CDS
TCTACACGGCTGGGTTGTGG+TGG 0.587866 2:-13785375 None:intergenic
GTGGTGATCAGGTGTCTCAA+GGG 0.590017 2:-13785888 None:intergenic
AACTAAAGAATCGGTTATGG+CGG 0.592037 2:+13786040 MsaT007101.1:CDS
GACTTCATCAGTATGAGGAT+CGG 0.593232 2:-13785586 None:intergenic
GAAACAATTTCATCACCTGT+AGG 0.594064 2:-13785684 None:intergenic
ATATATATTATAGGTTAACT+GGG 0.595356 2:+13787274 MsaT007101.1:intron
AGGGAGTTCCACGGTAGACA+TGG 0.596664 2:-13785869 None:intergenic
CTATCCCTTCTTGATGGTCA+CGG 0.597434 2:+13785522 MsaT007101.1:CDS
AAGGACCGTGACCATCAAGA+AGG 0.601243 2:-13785527 None:intergenic
TGTTTAGTTTCGAGGTCAAG+CGG 0.603245 2:-13785792 None:intergenic
GGTAAGATGATACATCCTGT+TGG 0.604159 2:+13787785 MsaT007101.1:CDS
TAGTTTCGAGGTCAAGCGGT+GGG 0.611231 2:-13785788 None:intergenic
TGAACACAACCATCAGTGAT+GGG 0.614197 2:-13785630 None:intergenic
GCATGTTCTAAGTGACCTTG+CGG 0.616291 2:-13785471 None:intergenic
GCCAAATCTTTGGTTCTACA+CGG 0.618346 2:-13785389 None:intergenic
ATTGATGTCGCGCAAGAACG+AGG 0.619010 2:-13785762 None:intergenic
TCCTCTTCACGAACCTCAGT+AGG 0.621000 2:-13785660 None:intergenic
AGAGTTGGAAGTAGTTCCTG+TGG 0.623129 2:-13787558 None:intergenic
TATACCTCAAGCATGCGCCA+TGG 0.631418 2:-13786284 None:intergenic
TCCAAAGCAAAGCCCTTGGG+AGG 0.633549 2:+13787319 MsaT007101.1:CDS
AAATTAGTTCAACCTCCCAA+GGG 0.635245 2:-13787331 None:intergenic
CACCAAACTTAGTATGCCGA+CGG 0.637276 2:-13786007 None:intergenic
GTAACAGACAGTTCTTGAGA+TGG 0.639997 2:-13785816 None:intergenic
AGGACCGTGACCATCAAGAA+GGG 0.643039 2:-13785526 None:intergenic
CTATCTGATGCTAATAATGG+TGG 0.650418 2:+13785726 MsaT007101.1:CDS
TGTCGCGCAAGAACGAGGTA+CGG 0.658162 2:-13785757 None:intergenic
GACGAAAAGAAACTCGTCTG+CGG 0.661354 2:+13785930 MsaT007101.1:CDS
GGGTTGTGGTGGAGACATGG+CGG 0.664200 2:-13785364 None:intergenic
GAGTTTCGCCATGTCTACCG+TGG 0.667413 2:+13785861 MsaT007101.1:CDS
CGTCAAACTTTGTCTCAAAG+GGG 0.667527 2:-13787709 None:intergenic
TCAACGGCCGATACAATGCA+AGG 0.667965 2:-13785555 None:intergenic
AGGATCGGCGAGGTAATCAA+CGG 0.669436 2:-13785571 None:intergenic
GGTGTCTCAAGGGAGTTCCA+CGG 0.685317 2:-13785878 None:intergenic
CCAGACTTTGCCATGAACGT+CGG 0.685939 2:-13785838 None:intergenic
TTAGTTTCGAGGTCAAGCGG+TGG 0.704813 2:-13785789 None:intergenic
CATCAGTATGAGGATCGGCG+AGG 0.728455 2:-13785581 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 13785345 13787868 13785345 ID=MsaG007101
Chr2 mRNA 13785345 13787868 13785345 ID=MsaT007101.1;Parent=MsaG007101
Chr2 exon 13785345 13786301 13785345 ID=MsaT007101.1.exon1;Parent=MsaT007101.1
Chr2 CDS 13785345 13786301 13785345 ID=cds.MsaT007101.1;Parent=MsaT007101.1
Chr2 exon 13787287 13787868 13787287 ID=MsaT007101.1.exon2;Parent=MsaT007101.1
Chr2 CDS 13787287 13787868 13787287 ID=cds.MsaT007101.1;Parent=MsaT007101.1
Gene Sequence

>MsaT007101.1

ATGGTAGAACCAAATATTTCCGCCATGTCTCCACCACAACCCAGCCGTGTAGAACCAAAGATTTGGCGGTGCTGCGCCGGACACTCTGTCAAAATTCCTAAACTTCATTCAAAAGTTTACTATTTCCCGCAAGGTCACTTAGAACATGCTTGCCCTAATCCCCCCAACACTGAAACCCTATCCCTTCTTGATGGTCACGGTCCTTTCAATCCTTGCATTGTATCGGCCGTTGATTACCTCGCCGATCCTCATACTGATGAAGTCTTTGTCAAACTTCTCCTAACTCCCATCACTGATGGTTGTGTTCACGAACCTCCTACTGAGGTTCGTGAAGAGGAACCTACAGGTGATGAAATTGTTTCCTCTGGAAAAACTCTAACCCTATCTGATGCTAATAATGGTGGTGCATTCTCCGTACCTCGTTCTTGCGCGACATCAATCTTCCCACCGCTTGACCTCGAAACTAAACAGCCATCTCAAGAACTGTCTGTTACCGACGTTCATGGCAAAGTCTGGGAGTTTCGCCATGTCTACCGTGGAACTCCCTTGAGACACCTGATCACCACTAATTGGAGTAAGTTCGTGGACGAAAAGAAACTCGTCTGCGGCGATTCTGTAATTTTTATGAAAAAGAAATCATCTGGAGATATGTTTGTTGGAATCCGTCGGCATACTAAGTTTGGTGCTGCTAAGATAACTAAAGAATCGGTTATGGCGGCAGCGGAGTTGGCGGATAAGAATATCGCTTTTGAAGTTGAGTATTATCCTACGATTGAAGGGTTTTGTGATTTTGTGGTGGATGCCAAGGTTGTTGAGGATGCAATGAAGATTAACTGGAATTGTGGAATGAGAATTAAATTTTCTTTGAAGAATGATGATTATTCAAAGAGATGGTCAGTTTTTAAGGGTACAATATCTGCTCTCTCTGCTCCTAATCGTCCATGGCGCATGCTTGAGGTTAACTGGGATGGACCTGAAGTCTCACATAATCCAAAGCAAAGCCCTTGGGAGGTTGAACTAATTTCTTATATACCTGCACTGAATCTACAATTGCCCTCAGCAAAAAGGCTTAGAGTTGCTCAGGTTTATACCTCATTAGATAATATAGAGAGATCTTCCATTCCAATGATAGATTTCCAGCATTCAACAGCAAAATCCTTGAATCAAACATTGTTGGATTGTGATACTTTTATTGAGGGCATGCAGAGAGCCCAAGACAGTCTTTTTTCCACAGGAACTACTTCCAACTCTTTCAATGATAGGGCCAATGATTTCATTGAGAAAAATTCAATGGGAAGGTTGGATATTGTGTCAACTGAGCCAAACATTAGCACATCTATATTTGGTGACTTGTCACCTCTTAACCATAGCATTTTGAACCCCTTTGAGACAAAGTTTGACGGTCCCCACAAGTGCAGCACCAAAGAAGTTAATCCTGGTTCATTTATGCTATTTGGTAAGATGATACATCCTGTTGGAAGTAATTTGCATGATTCTGCTACCAAGGGAGAAGATGGTTGTATGGGCTGCAATAATTGA
Protein sequence

>MsaT007101.1

MVEPNISAMSPPQPSRVEPKIWRCCAGHSVKIPKLHSKVYYFPQGHLEHACPNPPNTETLSLLDGHGPFNPCIVSAVDYLADPHTDEVFVKLLLTPITDGCVHEPPTEVREEEPTGDEIVSSGKTLTLSDANNGGAFSVPRSCATSIFPPLDLETKQPSQELSVTDVHGKVWEFRHVYRGTPLRHLITTNWSKFVDEKKLVCGDSVIFMKKKSSGDMFVGIRRHTKFGAAKITKESVMAAAELADKNIAFEVEYYPTIEGFCDFVVDAKVVEDAMKINWNCGMRIKFSLKNDDYSKRWSVFKGTISALSAPNRPWRMLEVNWDGPEVSHNPKQSPWEVELISYIPALNLQLPSAKRLRVAQVYTSLDNIERSSIPMIDFQHSTAKSLNQTLLDCDTFIEGMQRAQDSLFSTGTTSNSFNDRANDFIEKNSMGRLDIVSTEPNISTSIFGDLSPLNHSILNPFETKFDGPHKCSTKEVNPGSFMLFGKMIHPVGSNLHDSATKGEDGCMGCNN*