AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007190 MtrunA17_Chr2g0290341 84.178 493 39 5 39 501 22 505 0.0 833
MsaG007190 MtrunA17_Chr2g0290321 70.577 537 100 7 18 501 19 550 0.0 739
MsaG007190 MtrunA17_Chr7g0239471 69.844 514 114 7 1 501 1 486 0.0 651
MsaG007190 MtrunA17_Chr3g0120841 45.155 547 229 8 18 499 21 561 3.26e-144 426
MsaG007190 MtrunA17_Chr3g0120231 74.038 208 45 2 302 501 2 208 2.62e-99 298
MsaG007190 MtrunA17_Chr3g0120201 48.263 259 126 4 247 499 6 262 1.30e-72 231
MsaG007190 MtrunA17_Chr3g0120221 55.114 176 75 2 6 179 4 177 1.82e-61 199
MsaG007190 MtrunA17_Chr7g0238851 72.619 84 20 2 14 97 1 81 2.35e-29 111
MsaG007190 MtrunA17_Chr3g0120181 75.862 58 14 0 40 97 2 59 4.77e-28 106
MsaG007190 MtrunA17_Chr3g0120191 56.410 78 34 0 109 186 11 88 7.42e-27 104
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007190 AT4G16420.2 55.306 490 188 8 18 501 18 482 0.0 529
MsaG007190 AT4G16420.1 55.102 490 193 8 18 501 18 486 0.0 526
MsaG007190 AT4G16420.3 54.898 490 193 8 18 501 18 485 0.0 525
MsaG007190 AT3G07740.1 40.481 541 253 8 18 502 20 547 6.07e-130 389
MsaG007190 AT3G07740.3 41.155 537 246 9 23 502 3 526 7.00e-129 385
MsaG007190 AT3G07740.4 39.964 548 253 9 18 502 20 554 4.28e-127 382
MsaG007190 AT3G07740.2 40.574 488 221 8 71 502 2 476 2.21e-113 343

Find 106 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTCATTTCTTGAGGAATT+TGG 0.149009 2:-14802846 None:intergenic
AGACAGAAGAATGCTTTATT+TGG 0.172431 2:+14801077 MsaT007190.1:CDS
TTTGGAGATAATTCAGAATT+TGG 0.173645 2:+14801095 MsaT007190.1:CDS
TCTCCAAAGCACGGAGATTT+TGG 0.194560 2:+14802813 MsaT007190.1:CDS
CAGTTCCTCTAATGCTTTCT+TGG 0.220518 2:-14799939 None:intergenic
AAATGTATGGCCTGGAAAAT+TGG 0.221477 2:+14802188 MsaT007190.1:CDS
TCTTCATCTGCACTCCAGTC+TGG 0.242682 2:-14802009 None:intergenic
TCAGCATCATCATCATATTC+AGG 0.289106 2:-14802921 None:intergenic
TTCGGAACCTTGTGATTGTT+CGG 0.301945 2:-14803582 None:intergenic
AAGGAGCAGGTGAAGCAAAA+TGG 0.330889 2:+14801560 MsaT007190.1:CDS
GCATGAACACGTCATATTTC+CGG 0.336745 2:-14803256 None:intergenic
CCTCCAAAATCTCCGTGCTT+TGG 0.341129 2:-14802816 None:intergenic
TTATACGTATCTATAATAAA+AGG 0.343925 2:+14803012 MsaT007190.1:CDS
ACAAAAGCAATCATGATTAC+AGG 0.344529 2:+14801707 MsaT007190.1:CDS
CTCTCCGGTCTTCCCTCTTC+CGG 0.360280 2:+14802285 MsaT007190.1:CDS
ACCATCCAAGAAAGCATTAG+AGG 0.372484 2:+14799934 MsaT007190.1:CDS
TCGGAACCTTGTGATTGTTC+GGG 0.376591 2:-14803581 None:intergenic
TGCAGCTGGTGCTAACAAAA+AGG 0.378782 2:+14802664 MsaT007190.1:CDS
TCCTCTAATGCTTTCTTGGA+TGG 0.379688 2:-14799935 None:intergenic
CTTCATCTGCACTCCAGTCT+GGG 0.385677 2:-14802008 None:intergenic
TCAACACATAAGTCAAAATC+AGG 0.387672 2:-14801652 None:intergenic
AGCTGAAGCAGATATATATC+TGG 0.388984 2:+14803494 MsaT007190.1:CDS
GGTGTTAAATCCTTCTCAAC+TGG 0.395197 2:-14803215 None:intergenic
CCCGGGAAAAGCTAGTTTGC+TGG 0.399949 2:+14802700 MsaT007190.1:CDS
GAATTTGGTGCTGCTGCTCC+AGG 0.409359 2:+14801110 MsaT007190.1:CDS
GGATTTAACACCCGAGGAAA+AGG 0.418496 2:+14803226 MsaT007190.1:CDS
ATGCTCATTAAGAAGGGTAT+TGG 0.420108 2:+14803929 MsaT007190.1:CDS
AAGCTGAAGGACGTGCTCGC+AGG 0.426743 2:+14803535 MsaT007190.1:CDS
CCGAATGCATTGATGTCTGC+AGG 0.431685 2:+14803600 MsaT007190.1:CDS
AACGTTTGTGCTGCGAGTTA+AGG 0.442285 2:+14803771 MsaT007190.1:CDS
TGAGAAATTACTCGCTGATA+TGG 0.449215 2:+14802941 MsaT007190.1:CDS
TTCCTCTCATCTCCCCAGAC+TGG 0.451454 2:+14801995 MsaT007190.1:CDS
CTGTCGGTACAAATGATTGC+TGG 0.454974 2:+14803830 MsaT007190.1:CDS
GGGAATTGAAATGTATGGCC+TGG 0.457047 2:+14802180 MsaT007190.1:intron
AAGAAATGAAGGCCGTACTT+TGG 0.471852 2:+14802857 MsaT007190.1:CDS
GAACTTCTTGCAACGGCGGA+AGG 0.472081 2:+14802434 MsaT007190.1:CDS
AGGAGCAGGTGAAGCAAAAT+GGG 0.476294 2:+14801561 MsaT007190.1:CDS
AATGTATGGCCTGGAAAATT+GGG 0.480655 2:+14802189 MsaT007190.1:CDS
CTGCAGGAAGCAATTGCTGC+TGG 0.486220 2:+14803459 MsaT007190.1:intron
TTGATCCCATGAAAATTGAC+AGG 0.487796 2:+14803897 MsaT007190.1:CDS
TACTGCAAGAAAGATATTAC+CGG 0.489315 2:+14801601 MsaT007190.1:CDS
GTCAACACACATGCTGCAGC+TGG 0.495214 2:+14802650 MsaT007190.1:CDS
AGGGTCGGATTCTGGGCCCT+CGG 0.495801 2:+14802628 MsaT007190.1:intron
GTATGACATGCTCATTAAGA+AGG 0.500449 2:+14803922 MsaT007190.1:CDS
AAAGGCATCCAATAAAGGCC+CGG 0.500851 2:+14802682 MsaT007190.1:CDS
AGCTGAAGGACGTGCTCGCA+GGG 0.501164 2:+14803536 MsaT007190.1:CDS
CTTGAACATTTGATGAGCAT+CGG 0.502400 2:-14803870 None:intergenic
AGGACATGTCTCATGCTGTT+GGG 0.502752 2:+14802399 MsaT007190.1:intron
TTCTTGGCATAAACCTCCAT+CGG 0.503817 2:-14799908 None:intergenic
GAAGCCTATCATACCGGAAG+AGG 0.505742 2:-14802298 None:intergenic
CAGTTCTGAGCAATTCATCA+TGG 0.506351 2:-14803298 None:intergenic
CCTGCAGACATCAATGCATT+CGG 0.509384 2:-14803600 None:intergenic
GAGAATCTGTCAATGGCAAA+AGG 0.511677 2:-14802794 None:intergenic
CAGCAATCATTTGTACCGAC+AGG 0.513175 2:-14803829 None:intergenic
TAGGACATGTCTCATGCTGT+TGG 0.513693 2:+14802398 MsaT007190.1:intron
CCAAATTCCTCAAGAAATGA+AGG 0.515725 2:+14802846 MsaT007190.1:CDS
CTTGCAACGGCGGAAGGGCA+AGG 0.527237 2:+14802440 MsaT007190.1:CDS
GGCACACGGCACACTTGATA+CGG 0.529984 2:-14801630 None:intergenic
TGGTTATAACCCGAAAAGAC+AGG 0.531230 2:+14802890 MsaT007190.1:CDS
CTCTTGCATCTTCAGATACA+CGG 0.533130 2:-14803804 None:intergenic
TTCATCTGCACTCCAGTCTG+GGG 0.535702 2:-14802007 None:intergenic
TTGACAGCACTTGAAGTAGC+TGG 0.541031 2:-14803624 None:intergenic
TAAGAACAAAGAGGCATGCA+TGG 0.541880 2:+14802237 MsaT007190.1:CDS
AAGGCATCCAATAAAGGCCC+GGG 0.550057 2:+14802683 MsaT007190.1:CDS
AGTACGGCCTTCATTTCTTG+AGG 0.551492 2:-14802853 None:intergenic
CATACCGGAAGAGGGAAGAC+CGG 0.552809 2:-14802289 None:intergenic
TGCTTTGGAGAATCTGTCAA+TGG 0.554735 2:-14802801 None:intergenic
TGCATCTTCAGATACACGGT+TGG 0.556814 2:-14803800 None:intergenic
AACTTCTTGCAACGGCGGAA+GGG 0.560095 2:+14802435 MsaT007190.1:CDS
CACTGGCTTCAACCAAAGTA+CGG 0.564700 2:-14802869 None:intergenic
TCTGGCAAATAAGAGAAGAA+AGG 0.569800 2:+14803512 MsaT007190.1:CDS
CGTACTTTGGTTGAAGCCAG+TGG 0.574934 2:+14802870 MsaT007190.1:CDS
TGTGAGATAGGTCAAGGAGC+AGG 0.575240 2:+14801547 MsaT007190.1:intron
ATATGAAAATCCAGTTGAGA+AGG 0.580164 2:+14803205 MsaT007190.1:CDS
CCGAGGAAAAGGCAATATGC+CGG 0.591718 2:+14803237 MsaT007190.1:CDS
AAGAGAAGAAAGGAAGCTGA+AGG 0.596906 2:+14803522 MsaT007190.1:CDS
TGAAGAAATGTTGATTCTAG+AGG 0.597822 2:+14802026 MsaT007190.1:CDS
CAAAAGCAATCATGATTACA+GGG 0.598809 2:+14801708 MsaT007190.1:CDS
CAATCATGATTACAGGGTCA+TGG 0.603132 2:+14801714 MsaT007190.1:CDS
GGCTCGATGAAAGAGAGCGC+AGG 0.603188 2:+14803153 MsaT007190.1:intron
TGCAGCATGTGTGTTGACCG+AGG 0.608087 2:-14802645 None:intergenic
GCAGCATGTGTGTTGACCGA+GGG 0.612844 2:-14802644 None:intergenic
CAGAGAAGAACTTCTTGCAA+CGG 0.616082 2:+14802427 MsaT007190.1:CDS
AAGCCTATCATACCGGAAGA+GGG 0.618355 2:-14802297 None:intergenic
GCAGAAGTAGCTGAGCATGT+TGG 0.618695 2:+14802211 MsaT007190.1:CDS
TATGACATGCTCATTAAGAA+GGG 0.622187 2:+14803923 MsaT007190.1:CDS
CTCCAGTCTGGGGAGATGAG+AGG 0.625328 2:-14801997 None:intergenic
AGAAGAACTTCTTGCAACGG+CGG 0.628446 2:+14802430 MsaT007190.1:CDS
GGAAAAGCTAGTTTGCTGGA+AGG 0.630114 2:+14802704 MsaT007190.1:CDS
GTTCATGCGCTTTCATACCA+AGG 0.639075 2:+14803271 MsaT007190.1:CDS
TTGACAGATTCTCCAAAGCA+CGG 0.643030 2:+14802804 MsaT007190.1:CDS
CGAATGCATTGATGTCTGCA+GGG 0.644855 2:+14803601 MsaT007190.1:CDS
TGAGAAGGATTTAACACCCG+AGG 0.647022 2:+14803220 MsaT007190.1:CDS
TAAGTCAAAATCAGGGCACA+CGG 0.654683 2:-14801644 None:intergenic
GGCAAGAGAAAGTACTCATG+TGG 0.657734 2:+14803557 MsaT007190.1:CDS
GAAGATGCAAGAGGACCTGT+CGG 0.659393 2:+14803814 MsaT007190.1:CDS
CGTGTATCTGAAGATGCAAG+AGG 0.659698 2:+14803805 MsaT007190.1:CDS
AGATTAAGAGAGATCTCAGG+AGG 0.659721 2:-14803954 None:intergenic
TAATGACAATGACACTGAGG+AGG 0.665463 2:+14802968 MsaT007190.1:CDS
ACACTTGATACGGATTACTC+CGG 0.666906 2:-14801620 None:intergenic
CAACACATAAGTCAAAATCA+GGG 0.668458 2:-14801651 None:intergenic
TATCTGCTTCAGCTGAAGTG+CGG 0.668725 2:-14803485 None:intergenic
TGATCCCATGAAAATTGACA+GGG 0.695744 2:+14803898 MsaT007190.1:CDS
TGTTGGAACTAAGAACAAAG+AGG 0.697393 2:+14802228 MsaT007190.1:CDS
TGTGGTCCCGAACAATCACA+AGG 0.700460 2:+14803575 MsaT007190.1:CDS
GTTTAATGACAATGACACTG+AGG 0.755990 2:+14802965 MsaT007190.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 14799911 14803961 14799911 ID=MsaG007190
Chr2 mRNA 14799911 14803961 14799911 ID=MsaT007190.1;Parent=MsaG007190
Chr2 exon 14799911 14799960 14799911 ID=MsaT007190.1.exon1;Parent=MsaT007190.1
Chr2 CDS 14799911 14799960 14799911 ID=cds.MsaT007190.1;Parent=MsaT007190.1
Chr2 exon 14801070 14801131 14801070 ID=MsaT007190.1.exon2;Parent=MsaT007190.1
Chr2 CDS 14801070 14801131 14801070 ID=cds.MsaT007190.1;Parent=MsaT007190.1
Chr2 exon 14801557 14801735 14801557 ID=MsaT007190.1.exon3;Parent=MsaT007190.1
Chr2 CDS 14801557 14801735 14801557 ID=cds.MsaT007190.1;Parent=MsaT007190.1
Chr2 exon 14801982 14802047 14801982 ID=MsaT007190.1.exon4;Parent=MsaT007190.1
Chr2 CDS 14801982 14802047 14801982 ID=cds.MsaT007190.1;Parent=MsaT007190.1
Chr2 exon 14802181 14802306 14802181 ID=MsaT007190.1.exon5;Parent=MsaT007190.1
Chr2 CDS 14802181 14802306 14802181 ID=cds.MsaT007190.1;Parent=MsaT007190.1
Chr2 exon 14802401 14802461 14802401 ID=MsaT007190.1.exon6;Parent=MsaT007190.1
Chr2 CDS 14802401 14802461 14802401 ID=cds.MsaT007190.1;Parent=MsaT007190.1
Chr2 exon 14802630 14802725 14802630 ID=MsaT007190.1.exon7;Parent=MsaT007190.1
Chr2 CDS 14802630 14802725 14802630 ID=cds.MsaT007190.1;Parent=MsaT007190.1
Chr2 exon 14802793 14803033 14802793 ID=MsaT007190.1.exon8;Parent=MsaT007190.1
Chr2 CDS 14802793 14803033 14802793 ID=cds.MsaT007190.1;Parent=MsaT007190.1
Chr2 exon 14803154 14803364 14803154 ID=MsaT007190.1.exon9;Parent=MsaT007190.1
Chr2 CDS 14803154 14803364 14803154 ID=cds.MsaT007190.1;Parent=MsaT007190.1
Chr2 exon 14803465 14803686 14803465 ID=MsaT007190.1.exon10;Parent=MsaT007190.1
Chr2 CDS 14803465 14803686 14803465 ID=cds.MsaT007190.1;Parent=MsaT007190.1
Chr2 exon 14803767 14803961 14803767 ID=MsaT007190.1.exon11;Parent=MsaT007190.1
Chr2 CDS 14803767 14803961 14803767 ID=cds.MsaT007190.1;Parent=MsaT007190.1
Gene Sequence

>MsaT007190.1

ATGGAGGTTTATGCCAAGAAAAAACCATCCAAGAAAGCATTAGAGGAACTGGCAAGGAGACAGAAGAATGCTTTATTTGGAGATAATTCAGAATTTGGTGCTGCTGCTCCAGGTCAAGGAGCAGGTGAAGCAAAATGGGCTCTGTATCATTGCAATTACTGCAAGAAAGATATTACCGGAGTAATCCGTATCAAGTGTGCCGTGTGCCCTGATTTTGACTTATGTGTTGAGTGCTTTTCTGTTGGAGCTGAGCTCACACCCCACAAAAGCAATCATGATTACAGGGTCATGAATAAATTGTCTTTTCCTCTCATCTCCCCAGACTGGAGTGCAGATGAAGAAATGTTGATTCTAGAGGGAATTGAAATGTATGGCCTGGAAAATTGGGCAGAAGTAGCTGAGCATGTTGGAACTAAGAACAAAGAGGCATGCATGGAACACTATAAGAAGTTTTACTTGAACTCTCCGGTCTTCCCTCTTCCGGACATGTCTCATGCTGTTGGGAGAAGCAGAGAAGAACTTCTTGCAACGGCGGAAGGGCAAGGGTCGGATTCTGGGCCCTCGGTCAACACACATGCTGCAGCTGGTGCTAACAAAAAGGCATCCAATAAAGGCCCGGGAAAAGCTAGTTTGCTGGAAGTCCTTTTGCCATTGACAGATTCTCCAAAGCACGGAGATTTTGGAGGAAATAAGCCAAATTCCTCAAGAAATGAAGGCCGTACTTTGGTTGAAGCCAGTGGTTATAACCCGAAAAGACAGGAATTCAATCCTGAATATGATGATGATGCTGAGAAATTACTCGCTGATATGGAGTTTAATGACAATGACACTGAGGAGGAGATAGAGCTCAAACTTCGTATTATACGTATCTATAATAAAAGGCTCGATGAAAGAGAGCGCAGGAAGAAATTCATACTTGAAAGAAATTTGCTATATGAAAATCCAGTTGAGAAGGATTTAACACCCGAGGAAAAGGCAATATGCCGGAAATATGACGTGTTCATGCGCTTTCATACCAAGGAAAACCATGATGAATTGCTCAGAACTGTTATCTCTGAACATAGATATCTGAAAAAGATTCTAGAGACTAAGGAAGCAATTGCTGCTGGTTGCCGCACTTCAGCTGAAGCAGATATATATCTGGCAAATAAGAGAAGAAAGGAAGCTGAAGGACGTGCTCGCAGGGCAAGAGAAAGTACTCATGTGGTCCCGAACAATCACAAGGTTCCGAATGCATTGATGTCTGCAGGGCCAGCTACTTCAAGTGCTGTCAATGCAACAAAACACTCAACAGCAGATTTACTTTCTGAAGTTGAGAAACGTTTGTGCTGCGAGTTAAGGTTGTCTCCAACCGTGTATCTGAAGATGCAAGAGGACCTGTCGGTACAAATGATTGCTGGAAGCGTCTCTTCAAAGTCCGATGCTCATCAAATGTTCAAGAATATTGATCCCATGAAAATTGACAGGGTGTATGACATGCTCATTAAGAAGGGTATTGGTTCCTCCTGA
Protein sequence

>MsaT007190.1

MEVYAKKKPSKKALEELARRQKNALFGDNSEFGAAAPGQGAGEAKWALYHCNYCKKDITGVIRIKCAVCPDFDLCVECFSVGAELTPHKSNHDYRVMNKLSFPLISPDWSADEEMLILEGIEMYGLENWAEVAEHVGTKNKEACMEHYKKFYLNSPVFPLPDMSHAVGRSREELLATAEGQGSDSGPSVNTHAAAGANKKASNKGPGKASLLEVLLPLTDSPKHGDFGGNKPNSSRNEGRTLVEASGYNPKRQEFNPEYDDDAEKLLADMEFNDNDTEEEIELKLRIIRIYNKRLDERERRKKFILERNLLYENPVEKDLTPEEKAICRKYDVFMRFHTKENHDELLRTVISEHRYLKKILETKEAIAAGCRTSAEADIYLANKRRKEAEGRARRARESTHVVPNNHKVPNALMSAGPATSSAVNATKHSTADLLSEVEKRLCCELRLSPTVYLKMQEDLSVQMIAGSVSSKSDAHQMFKNIDPMKIDRVYDMLIKKGIGSS*