AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007208

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007208 MtrunA17_Chr2g0290691 91.772 158 8 1 6 158 379 536 5.30e-99 296
MsaG007208 MtrunA17_Chr6g0449401 74.510 153 37 2 6 157 473 624 5.61e-76 239
MsaG007208 MtrunA17_Chr7g0227331 75.000 160 31 3 6 157 405 563 1.20e-74 234
MsaG007208 MtrunA17_Chr8g0335351 73.203 153 41 0 5 157 543 695 2.09e-71 228
MsaG007208 MtrunA17_Chr7g0250631 71.429 154 42 2 5 157 536 688 3.14e-70 225
MsaG007208 MtrunA17_Chr8g0356291 68.085 141 42 2 18 157 314 452 2.21e-61 197
MsaG007208 MtrunA17_Chr2g0309421 69.565 138 38 3 22 157 365 500 2.97e-58 190
MsaG007208 MtrunA17_Chr1g0179101 63.226 155 50 3 7 157 644 795 1.70e-57 192
MsaG007208 MtrunA17_Chr7g0266031 61.438 153 54 3 8 157 667 817 1.12e-53 181
MsaG007208 MtrunA17_Chr3g0080101 57.792 154 60 3 7 157 591 742 5.01e-52 176
MsaG007208 MtrunA17_Chr2g0321101 48.344 151 67 1 8 158 277 416 4.21e-40 140
MsaG007208 MtrunA17_Chr4g0063311 50.314 159 61 4 7 152 273 426 2.37e-37 133
MsaG007208 MtrunA17_Chr8g0381091 49.645 141 58 3 20 157 267 397 4.48e-37 132
MsaG007208 MtrunA17_Chr2g0285711 46.000 150 68 3 11 157 260 399 1.35e-36 130
MsaG007208 MtrunA17_Chr4g0030361 44.366 142 67 5 17 158 456 585 2.31e-27 106
MsaG007208 MtrunA17_Chr2g0312641 50.459 109 50 2 53 157 286 394 2.69e-27 105
MsaG007208 MtrunA17_Chr8g0369091 48.571 105 53 1 54 158 775 878 1.50e-26 104
MsaG007208 MtrunA17_Chr2g0327561 49.524 105 52 1 54 158 812 915 1.33e-25 102
MsaG007208 MtrunA17_Chr2g0329011 44.355 124 65 2 35 157 186 306 1.11e-22 92.4
MsaG007208 MtrunA17_Chr8g0338741 47.778 90 44 1 62 151 246 332 6.37e-22 90.9
MsaG007208 MtrunA17_Chr4g0032761 47.917 96 47 1 62 157 250 342 7.52e-22 90.5
MsaG007208 MtrunA17_Chr3g0125381 42.268 97 56 0 56 152 100 196 4.69e-21 85.5
MsaG007208 MtrunA17_Chr5g0428981 40.659 91 53 1 62 151 947 1037 6.46e-18 79.7
MsaG007208 MtrunA17_Chr4g0018201 40.659 91 53 1 62 151 943 1033 7.61e-18 79.7
MsaG007208 MtrunA17_Chr4g0059751 37.113 97 59 1 62 158 249 343 1.39e-16 75.9
MsaG007208 MtrunA17_Chr7g0234641 42.222 90 49 1 62 151 237 323 1.74e-16 75.5
MsaG007208 MtrunA17_Chr3g0140631 37.634 93 58 0 59 151 185 277 1.92e-16 74.7
MsaG007208 MtrunA17_Chr8g0351491 42.529 87 50 0 65 151 170 256 6.68e-16 72.8
MsaG007208 MtrunA17_Chr8g0353621 38.000 100 61 1 56 155 156 254 6.93e-16 72.8
MsaG007208 MtrunA17_Chr6g0478601 38.298 94 57 1 62 154 296 389 2.43e-15 72.4
MsaG007208 MtrunA17_Chr7g0245031 42.391 92 51 1 61 152 300 389 4.05e-15 71.6
MsaG007208 MtrunA17_Chr3g0135951 37.273 110 64 3 49 154 282 390 1.36e-13 67.4
MsaG007208 MtrunA17_Chr1g0170551 33.708 89 57 1 65 153 162 248 3.54e-13 66.2
MsaG007208 MtrunA17_Chr1g0153321 37.931 116 63 4 36 143 273 387 9.29e-13 64.7
MsaG007208 MtrunA17_Chr1g0167351 39.130 92 53 2 65 154 309 399 5.99e-11 59.7
MsaG007208 MtrunA17_Chr1g0183651 33.065 124 72 3 33 154 293 407 6.45e-11 59.7
MsaG007208 MtrunA17_Chr1g0190131 33.000 100 64 2 60 156 159 258 9.02e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007208 AT5G47750.1 81.013 158 26 1 5 158 362 519 4.56e-87 266
MsaG007208 AT4G26610.1 85.714 154 21 1 6 158 295 448 1.51e-83 254
MsaG007208 AT5G55910.2 82.911 158 23 3 5 158 281 438 1.02e-78 242
MsaG007208 AT5G55910.1 82.911 158 23 3 5 158 281 438 1.02e-78 242
MsaG007208 AT3G27580.2 67.949 156 47 1 6 158 353 508 2.39e-70 222
MsaG007208 AT3G27580.1 67.949 156 47 1 6 158 353 508 2.39e-70 222
MsaG007208 AT5G40030.1 64.286 154 50 4 6 157 284 434 8.22e-61 196
MsaG007208 AT1G79250.1 69.504 141 40 3 18 157 333 471 8.65e-60 194
MsaG007208 AT1G79250.3 69.504 141 40 3 18 157 333 471 8.65e-60 194
MsaG007208 AT1G79250.2 69.504 141 40 3 18 157 333 471 8.65e-60 194
MsaG007208 AT1G79250.4 69.504 141 40 3 18 157 333 471 8.65e-60 194
MsaG007208 AT3G12690.1 68.571 140 41 2 18 157 364 500 3.83e-58 190
MsaG007208 AT3G12690.3 68.571 140 41 2 18 157 364 500 3.83e-58 190
MsaG007208 AT3G12690.2 68.571 140 41 2 18 157 364 500 3.83e-58 190
MsaG007208 AT2G44830.2 62.893 159 53 4 5 158 532 689 4.38e-57 190
MsaG007208 AT2G44830.3 62.893 159 53 4 5 158 532 689 4.38e-57 190
MsaG007208 AT2G44830.1 62.893 159 53 4 5 158 532 689 4.38e-57 190
MsaG007208 AT3G52890.2 58.710 155 57 2 7 156 717 869 7.64e-54 182
MsaG007208 AT3G52890.4 58.710 155 57 2 7 156 717 869 7.64e-54 182
MsaG007208 AT3G52890.1 58.710 155 57 2 7 156 717 869 7.64e-54 182
MsaG007208 AT3G52890.3 58.710 155 57 2 7 156 717 869 7.64e-54 182
MsaG007208 AT2G36350.1 58.710 155 57 3 7 157 738 889 8.33e-53 179
MsaG007208 AT1G16440.1 75.238 105 26 0 53 157 323 427 7.14e-52 172
MsaG007208 AT5G03640.2 58.333 156 48 3 12 157 721 869 1.24e-49 171
MsaG007208 AT5G03640.1 58.333 156 48 3 12 157 721 869 1.24e-49 171
MsaG007208 AT3G44610.1 53.472 144 57 4 16 158 271 405 2.40e-43 149
MsaG007208 AT2G26700.1 41.969 193 66 6 7 157 268 456 6.63e-36 130
MsaG007208 AT2G34650.1 44.853 136 71 3 26 157 250 385 2.08e-30 114
MsaG007208 AT5G58140.6 43.066 137 71 2 22 158 727 856 1.01e-28 111
MsaG007208 AT5G58140.7 43.066 137 71 2 22 158 727 856 1.01e-28 111
MsaG007208 AT5G58140.5 43.066 137 71 2 22 158 727 856 1.01e-28 111
MsaG007208 AT5G58140.2 43.066 137 71 2 22 158 727 856 1.01e-28 111
MsaG007208 AT5G58140.1 43.066 137 71 2 22 158 727 856 1.01e-28 111
MsaG007208 AT5G58140.3 43.066 137 71 2 22 158 727 856 1.23e-28 110
MsaG007208 AT3G14370.1 50.442 113 43 2 53 158 282 388 1.07e-26 104
MsaG007208 AT1G53700.1 50.467 107 52 1 53 158 286 392 2.82e-26 103
MsaG007208 AT3G45780.2 48.571 105 53 1 54 158 841 944 2.43e-24 98.6
MsaG007208 AT3G45780.1 48.571 105 53 1 54 158 841 944 2.43e-24 98.6
MsaG007208 AT3G25250.1 45.361 97 50 1 55 151 225 318 2.63e-23 94.7
MsaG007208 AT1G51170.1 40.909 132 70 3 26 157 208 331 4.18e-23 94.4
MsaG007208 AT4G13000.1 40.972 144 74 5 15 151 172 311 4.83e-22 90.9
MsaG007208 AT3G20830.1 47.917 96 47 1 62 157 240 332 5.75e-22 91.3
MsaG007208 AT3G08720.1 44.086 93 49 2 60 152 291 380 2.82e-18 80.9
MsaG007208 AT3G08720.3 44.086 93 49 2 60 152 291 380 2.82e-18 80.9
MsaG007208 AT3G08720.4 44.086 93 49 2 60 152 291 380 2.82e-18 80.9
MsaG007208 AT3G08720.2 44.086 93 49 2 60 152 291 380 2.82e-18 80.9
MsaG007208 AT3G08730.1 43.011 93 50 2 60 152 285 374 8.39e-18 79.7
MsaG007208 AT3G08730.2 43.011 93 50 2 60 152 285 374 8.39e-18 79.7
MsaG007208 AT2G25880.2 40.426 94 56 0 59 152 141 234 2.13e-17 77.0
MsaG007208 AT2G45490.1 40.659 91 54 0 65 155 176 266 2.53e-17 77.0
MsaG007208 AT2G25880.4 40.426 94 56 0 59 152 186 279 3.55e-17 77.0
MsaG007208 AT2G25880.1 40.426 94 56 0 59 152 173 266 3.77e-17 76.6
MsaG007208 AT1G48490.4 38.136 118 67 2 43 154 984 1101 4.53e-17 77.8
MsaG007208 AT1G48490.3 38.136 118 67 2 43 154 983 1100 4.66e-17 77.8
MsaG007208 AT1G48490.2 38.136 118 67 2 43 154 983 1100 4.66e-17 77.8
MsaG007208 AT1G48490.1 38.136 118 67 2 43 154 983 1100 4.66e-17 77.8
MsaG007208 AT2G25880.3 40.426 94 56 0 59 152 234 327 7.43e-17 76.6
MsaG007208 AT4G32830.1 38.710 93 57 0 59 151 179 271 1.57e-16 75.1
MsaG007208 AT1G45160.1 44.944 89 48 1 65 152 854 942 2.25e-15 72.8
MsaG007208 AT1G45160.2 44.944 89 48 1 65 152 854 942 2.30e-15 72.8
MsaG007208 AT5G62310.1 37.234 94 58 1 65 157 942 1035 4.01e-15 72.4
MsaG007208 AT3G17850.1 35.652 115 72 2 42 154 1046 1160 1.04e-14 70.9
MsaG007208 AT2G45490.2 39.241 79 48 0 65 143 176 254 9.53e-13 64.3
MsaG007208 AT5G09890.1 36.735 98 58 2 50 143 286 383 1.34e-12 64.7
MsaG007208 AT5G09890.2 36.735 98 58 2 50 143 286 383 1.36e-12 64.7
MsaG007208 AT1G50240.3 35.955 89 55 1 65 153 161 247 1.84e-12 64.7
MsaG007208 AT1G50240.2 35.955 89 55 1 65 153 161 247 1.84e-12 64.7
MsaG007208 AT1G12580.1 40.449 89 48 3 67 152 206 292 1.46e-11 62.0
MsaG007208 AT2G20470.3 37.349 83 50 1 63 143 318 400 2.03e-11 61.2
MsaG007208 AT2G20470.2 38.272 81 48 1 65 143 320 400 2.27e-11 61.2
MsaG007208 AT2G20470.1 38.272 81 48 1 65 143 320 400 2.27e-11 61.2
MsaG007208 AT1G30640.3 34.906 106 64 3 53 154 215 319 7.91e-11 59.7
MsaG007208 AT1G30640.2 34.737 95 58 2 53 143 308 402 8.33e-11 59.7
MsaG007208 AT1G30640.1 34.737 95 58 2 53 143 308 402 8.33e-11 59.7

Find 50 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTGGCAAAACTCCGTTTA+AGG 0.192811 2:+15001135 MsaT007208.1:CDS
TTGCAGAAAGGGATCTTATC+AGG 0.265548 2:+15001233 MsaT007208.1:CDS
TTGATTGCCAAGCTCGGTTT+TGG 0.289209 2:-15000954 None:intergenic
GCAGTTGATTGTTGGACTTA+TGG 0.319968 2:+15001091 MsaT007208.1:CDS
AAGCTAACAACAGGTGCTTC+TGG 0.322983 2:-15001211 None:intergenic
AAACGGTGTGGCTCGGTTCC+CGG 0.333246 2:-15001161 None:intergenic
TTTCTATACGAGTTGTTGTT+TGG 0.373039 2:+15001118 MsaT007208.1:CDS
TTTGGCAAAACTCCGTTTAA+GGG 0.411556 2:+15001136 MsaT007208.1:CDS
CCTTCACCCTTGATTATCTC+AGG 0.417264 2:-15001058 None:intergenic
AAAACTCCGTTTAAGGGATC+CGG 0.419047 2:+15001142 MsaT007208.1:CDS
TGTTGTTAGCTTTGCAGAAA+GGG 0.428583 2:+15001222 MsaT007208.1:CDS
AGAACTAGAGATGGAGCATT+GGG 0.430335 2:+15000826 None:intergenic
GGCTGACCTACCACGTTAAA+CGG 0.432552 2:-15001178 None:intergenic
CGCAAGGCCAAAACCGAGCT+TGG 0.443083 2:+15000947 MsaT007208.1:CDS
TGTTGGAACTCACGAATACC+TGG 0.462280 2:+15001033 MsaT007208.1:CDS
ACGGCAGTGCAGTTGATTGT+TGG 0.471044 2:+15001083 MsaT007208.1:CDS
AAAGGGATGTTGTTTAATCT+CGG 0.474175 2:-15001309 None:intergenic
TATCAGGGGTTTGCTTGTAA+AGG 0.485912 2:+15001249 MsaT007208.1:CDS
CTTTCTGCAAAGCTAACAAC+AGG 0.494011 2:-15001220 None:intergenic
CTGTTGTTAGCTTTGCAGAA+AGG 0.494754 2:+15001221 MsaT007208.1:CDS
AAAGGAACCTCAGCACAGAT+TGG 0.496667 2:+15001267 MsaT007208.1:CDS
CGGTTCCCGGATCCCTTAAA+CGG 0.499894 2:-15001148 None:intergenic
GACATAGAGCGAGCGTCGGT+GGG 0.522703 2:-15001007 None:intergenic
AGACATAGAGCGAGCGTCGG+TGG 0.528304 2:-15001008 None:intergenic
CACAGATTGGCTTATAAAAG+AGG 0.530338 2:+15001280 MsaT007208.1:CDS
ACTGACTTGATTGCCAAGCT+CGG 0.542542 2:-15000960 None:intergenic
TGCAGAAAGGGATCTTATCA+GGG 0.542643 2:+15001234 MsaT007208.1:CDS
CCTTGATTATCTCAGGTGCC+AGG 0.550487 2:-15001051 None:intergenic
CCTGGCACCTGAGATAATCA+AGG 0.551189 2:+15001051 MsaT007208.1:CDS
TAGAACTAGAGATGGAGCAT+TGG 0.559898 2:+15000825 None:intergenic
AAACTCCGTTTAAGGGATCC+GGG 0.560479 2:+15001143 MsaT007208.1:CDS
CCACGTTAAACGGTGTGGCT+CGG 0.563162 2:-15001168 None:intergenic
TTATAAGCCAATCTGTGCTG+AGG 0.571793 2:-15001274 None:intergenic
ATAATCAAGGGTGAAGGTCA+CGG 0.593639 2:+15001064 MsaT007208.1:CDS
CAAATCAAAGAAAGAACGCA+AGG 0.607512 2:+15000931 MsaT007208.1:CDS
GCAGAAAGGGATCTTATCAG+GGG 0.632689 2:+15001235 MsaT007208.1:CDS
ACCTACCACGTTAAACGGTG+TGG 0.635170 2:-15001173 None:intergenic
GATGGCGGCTCAATACATGC+AGG 0.638381 2:-15000863 None:intergenic
CCTGAGATAATCAAGGGTGA+AGG 0.640110 2:+15001058 MsaT007208.1:CDS
ACACGAGGTAGGAGCAACAC+AGG 0.648331 2:-15000885 None:intergenic
TCTGCAACGAGCTCAAGTCG+AGG 0.651611 2:-15000983 None:intergenic
GAGGTAGGAGCAACACAGGA+TGG 0.657527 2:-15000881 None:intergenic
CTGGCACCTGAGATAATCAA+GGG 0.661653 2:+15001052 MsaT007208.1:CDS
CCGAGCCACACCGTTTAACG+TGG 0.665030 2:+15001168 MsaT007208.1:CDS
AGATTGGCTTATAAAAGAGG+CGG 0.676279 2:+15001283 MsaT007208.1:CDS
GTAGGAGCAACACAGGATGG+CGG 0.676336 2:-15000878 None:intergenic
AAAAGACATAGAGCGAGCGT+CGG 0.699256 2:-15001011 None:intergenic
GCCACACCGTTTAACGTGGT+AGG 0.710142 2:+15001172 MsaT007208.1:CDS
GATTTGCTAGAAAAGAGCCG+AGG 0.729953 2:-15000914 None:intergenic
GGTGCTTCTGGAAATCGCAG+CGG 0.758291 2:-15001199 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 15000836 15001318 15000836 ID=MsaG007208
Chr2 mRNA 15000836 15001318 15000836 ID=MsaT007208.1;Parent=MsaG007208
Chr2 exon 15000836 15001318 15000836 ID=MsaT007208.1.exon1;Parent=MsaT007208.1
Chr2 CDS 15000836 15001318 15000836 ID=cds.MsaT007208.1;Parent=MsaT007208.1
Gene Sequence

>MsaT007208.1

ATGGAGCATTGGGTTTACTGTGCTCAGCCTGCATGTATTGAGCCGCCATCCTGTGTTGCTCCTACCTCGTGTTTTTCGCCTCGGCTCTTTTCTAGCAAATCAAAGAAAGAACGCAAGGCCAAAACCGAGCTTGGCAATCAAGTCAGTCCTCGACTTGAGCTCGTTGCAGAGCCCACCGACGCTCGCTCTATGTCTTTTGTTGGAACTCACGAATACCTGGCACCTGAGATAATCAAGGGTGAAGGTCACGGCAGTGCAGTTGATTGTTGGACTTATGGAATCTTTCTATACGAGTTGTTGTTTGGCAAAACTCCGTTTAAGGGATCCGGGAACCGAGCCACACCGTTTAACGTGGTAGGTCAGCCGCTGCGATTTCCAGAAGCACCTGTTGTTAGCTTTGCAGAAAGGGATCTTATCAGGGGTTTGCTTGTAAAGGAACCTCAGCACAGATTGGCTTATAAAAGAGGCGGCAACCGAGATTAA
Protein sequence

>MsaT007208.1

MEHWVYCAQPACIEPPSCVAPTSCFSPRLFSSKSKKERKAKTELGNQVSPRLELVAEPTDARSMSFVGTHEYLAPEIIKGEGHGSAVDCWTYGIFLYELLFGKTPFKGSGNRATPFNVVGQPLRFPEAPVVSFAERDLIRGLLVKEPQHRLAYKRGGNRD*