AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007233

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007233 MtrunA17_Chr2g0290491 99.398 166 1 0 1 166 1 166 5.70e-122 340
MsaG007233 MtrunA17_Chr5g0446491 66.061 165 43 4 1 155 1 162 1.01e-68 206
MsaG007233 MtrunA17_Chr3g0102351 73.239 142 23 3 1 132 1 137 6.66e-67 202
MsaG007233 MtrunA17_Chr8g0382931 76.190 126 23 2 16 134 27 152 1.27e-63 194
MsaG007233 MtrunA17_Chr4g0076321 73.077 130 30 2 1 125 1 130 5.21e-63 190
MsaG007233 MtrunA17_Chr7g0263601 73.950 119 22 1 1 110 1 119 1.50e-61 187
MsaG007233 MtrunA17_Chr1g0185911 64.539 141 40 2 3 133 9 149 1.92e-59 182
MsaG007233 MtrunA17_Chr8g0384451 77.778 108 22 1 16 121 39 146 3.71e-58 180
MsaG007233 MtrunA17_Chr4g0076381 52.564 156 54 4 2 144 48 196 8.11e-53 167
MsaG007233 MtrunA17_Chr1g0195851 75.000 96 24 0 16 111 35 130 8.28e-52 164
MsaG007233 MtrunA17_Chr2g0296321 59.091 132 45 3 4 133 57 181 6.93e-50 160
MsaG007233 MtrunA17_Chr4g0067091 67.619 105 31 1 4 108 12 113 1.07e-48 156
MsaG007233 MtrunA17_Chr1g0165041 62.745 102 38 0 13 114 2 103 1.17e-47 153
MsaG007233 MtrunA17_Chr1g0191981 64.130 92 33 0 17 108 4 95 7.69e-41 134
MsaG007233 MtrunA17_Chr1g0159271 53.571 84 39 0 31 114 1 84 5.14e-26 95.5
MsaG007233 MtrunA17_Chr5g0446601 43.119 109 59 1 1 106 5 113 4.49e-24 92.8
MsaG007233 MtrunA17_Chr1g0158951 46.429 84 44 1 47 129 19 102 1.45e-19 79.0
MsaG007233 MtrunA17_Chr1g0159291 40.789 76 45 0 22 97 7 82 9.37e-19 76.3
MsaG007233 MtrunA17_Chr2g0307551 33.333 78 52 0 18 95 12 89 1.53e-14 67.0
MsaG007233 MtrunA17_Chr4g0062271 32.530 83 56 0 13 95 7 89 2.49e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007233 AT4G14540.1 83.471 121 16 1 1 117 1 121 6.73e-71 211
MsaG007233 AT5G47640.1 83.929 112 16 2 16 127 26 135 6.21e-64 194
MsaG007233 AT2G38880.3 68.939 132 36 2 10 139 12 140 1.58e-60 184
MsaG007233 AT2G38880.2 70.312 128 33 2 10 135 12 136 2.17e-60 184
MsaG007233 AT2G38880.1 70.312 128 33 2 10 135 12 136 2.17e-60 184
MsaG007233 AT2G38880.5 70.312 128 33 2 10 135 12 136 2.17e-60 184
MsaG007233 AT2G37060.2 77.064 109 21 1 6 110 15 123 2.68e-59 182
MsaG007233 AT2G37060.3 77.064 109 21 1 6 110 15 123 2.68e-59 182
MsaG007233 AT2G37060.1 77.064 109 21 1 6 110 15 123 2.68e-59 182
MsaG007233 AT2G38880.7 69.531 128 32 2 10 135 12 134 1.04e-58 179
MsaG007233 AT3G53340.5 62.838 148 38 4 15 147 27 172 9.50e-58 178
MsaG007233 AT3G53340.1 62.838 148 38 4 15 147 27 172 9.50e-58 178
MsaG007233 AT2G13570.1 83.333 96 16 0 16 111 35 130 1.33e-57 179
MsaG007233 AT2G38880.4 81.000 100 17 1 10 107 12 111 1.44e-57 176
MsaG007233 AT2G38880.11 75.926 108 24 1 10 115 12 119 4.80e-57 176
MsaG007233 AT2G38880.8 75.926 108 24 1 10 115 12 119 4.80e-57 176
MsaG007233 AT2G38880.9 81.000 100 17 1 10 107 12 111 6.11e-57 176
MsaG007233 AT2G38880.10 81.000 100 17 1 10 107 12 111 6.11e-57 176
MsaG007233 AT2G38880.6 81.000 100 17 1 10 107 12 111 6.11e-57 176
MsaG007233 AT3G53340.3 77.778 99 22 0 16 114 28 126 6.08e-56 173
MsaG007233 AT3G53340.2 77.778 99 22 0 16 114 28 126 6.08e-56 173
MsaG007233 AT3G53340.4 80.435 92 18 0 16 107 28 119 2.99e-55 170
MsaG007233 AT2G47810.1 72.549 102 28 0 16 117 50 151 5.21e-52 163
MsaG007233 AT5G47670.2 63.063 111 39 1 3 111 13 123 2.44e-51 163
MsaG007233 AT5G47670.1 63.063 111 39 1 3 111 42 152 5.13e-51 163
MsaG007233 AT5G47670.3 63.063 111 39 1 3 111 42 152 5.13e-51 163
MsaG007233 AT1G21970.1 52.273 132 60 2 12 140 52 183 8.84e-47 152
MsaG007233 AT1G09030.1 61.458 96 37 0 17 112 3 98 4.09e-43 140
MsaG007233 AT5G08190.2 34.286 105 63 2 13 113 7 109 1.78e-17 75.5
MsaG007233 AT5G23090.4 32.692 104 64 2 13 112 7 108 9.32e-17 73.6
MsaG007233 AT5G08190.1 37.349 83 52 0 13 95 7 89 1.88e-16 72.8
MsaG007233 AT5G23090.5 33.000 100 61 2 13 108 7 104 6.91e-16 71.2
MsaG007233 AT5G23090.2 33.000 100 61 2 13 108 7 104 6.91e-16 71.2
MsaG007233 AT5G23090.1 33.000 100 61 2 13 108 7 104 6.91e-16 71.2
MsaG007233 AT5G23090.3 32.911 79 47 2 34 108 15 91 2.24e-11 58.9

Find 39 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGGTAGTGGTGGAGTTTA+TGG 0.223086 2:-15338736 MsaT007233.1:CDS
TCAACGGTGATGATCTTCTT+TGG 0.252720 2:-15338888 MsaT007233.1:CDS
TTTGAGATCTTTGCGTTTGC+TGG 0.274169 2:+15339009 None:intergenic
ATCCTACTCACGTTTGCTAT+TGG 0.294063 2:+15339048 None:intergenic
GGGAGTGGATCGCCATCTTC+TGG 0.311375 2:-15338652 MsaT007233.1:CDS
CGTGAGTAGGATCATGAAAA+AGG 0.331544 2:-15339037 MsaT007233.1:CDS
GAGTTTATCAGTTTCATAAC+AGG 0.346221 2:-15338952 MsaT007233.1:CDS
GGACATAATATGTATGGATC+TGG 0.358939 2:-15338673 MsaT007233.1:CDS
CTTTGGGCTATGACAACTCT+TGG 0.376889 2:-15338871 MsaT007233.1:CDS
CAACGGTGATGATCTTCTTT+GGG 0.389396 2:-15338887 MsaT007233.1:CDS
GGAGAGAAGAGTGCTATGAT+TGG 0.411557 2:-15338790 MsaT007233.1:CDS
CAGGTTGCAGAGAGCAAGAT+AGG 0.412786 2:-15339077 MsaT007233.1:CDS
GAGTCAGGAGGTGGTCAAAC+AGG 0.413478 2:-15339096 MsaT007233.1:CDS
AGTGGTGGAGTTTATGGTCA+TGG 0.452362 2:-15338730 MsaT007233.1:CDS
GGAGTTTATGGTCATGGAGT+TGG 0.458217 2:-15338724 MsaT007233.1:CDS
AGAAGAGTGCTATGATTGGT+AGG 0.483869 2:-15338786 MsaT007233.1:CDS
ATCATCATCATCATAGAAGA+TGG 0.485382 2:+15338697 None:intergenic
CTAGTACTCCTTCCAGAAGA+TGG 0.486834 2:+15338640 None:intergenic
ATCTTCTATGATGATGATGA+TGG 0.489435 2:-15338695 MsaT007233.1:CDS
GGTAGGAGTGATCAGAGAGA+TGG 0.493822 2:-15338769 MsaT007233.1:CDS
GGAAATGTTGGTGAGGGTAG+TGG 0.495110 2:-15338748 MsaT007233.1:CDS
GTTGAATCAGACAATGAGTC+AGG 0.495902 2:-15339111 MsaT007233.1:CDS
TTTGAGTAAGTATAGAGAGA+TGG 0.496858 2:-15338815 MsaT007233.1:CDS
ATGATGGGACATAATATGTA+TGG 0.500614 2:-15338679 MsaT007233.1:CDS
GATCAGAGAGATGGAAATGT+TGG 0.512395 2:-15338760 MsaT007233.1:CDS
GACATAATATGTATGGATCT+GGG 0.545156 2:-15338672 MsaT007233.1:CDS
AGAGATGGAAATGTTGGTGA+GGG 0.549342 2:-15338754 MsaT007233.1:CDS
ATAAGTGTCAGAAAGAGAAG+AGG 0.567471 2:-15338918 MsaT007233.1:CDS
AGTAAGTATAGAGAGATGGA+AGG 0.568189 2:-15338811 MsaT007233.1:CDS
AATGTTGGTGAGGGTAGTGG+TGG 0.579830 2:-15338745 MsaT007233.1:CDS
AATATGTATGGATCTGGGAG+TGG 0.582558 2:-15338667 MsaT007233.1:CDS
TTCCAATAGCAAACGTGAGT+AGG 0.595976 2:-15339050 MsaT007233.1:CDS
TCAGACAATGAGTCAGGAGG+TGG 0.599898 2:-15339105 MsaT007233.1:CDS
TTACTCAAATAAATCTTGAG+TGG 0.610816 2:+15338829 None:intergenic
GAGAAGAGGAAAACAATCAA+CGG 0.615125 2:-15338904 MsaT007233.1:CDS
GAGAGATGGAAATGTTGGTG+AGG 0.620088 2:-15338755 MsaT007233.1:CDS
GTGGATCGCCATCTTCTGGA+AGG 0.622809 2:-15338648 MsaT007233.1:CDS
TCTTCTATGATGATGATGAT+GGG 0.641124 2:-15338694 MsaT007233.1:CDS
GAATCAGACAATGAGTCAGG+AGG 0.682591 2:-15339108 MsaT007233.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 15338636 15339136 15338636 ID=MsaG007233
Chr2 mRNA 15338636 15339136 15338636 ID=MsaT007233.1;Parent=MsaG007233
Chr2 exon 15338636 15339136 15338636 ID=MsaT007233.1.exon1;Parent=MsaT007233.1
Chr2 CDS 15338636 15339136 15338636 ID=cds.MsaT007233.1;Parent=MsaT007233.1
Gene Sequence

>MsaT007233.1

ATGGTTGAATCAGACAATGAGTCAGGAGGTGGTCAAACAGGTTGCAGAGAGCAAGATAGGTTTCTTCCAATAGCAAACGTGAGTAGGATCATGAAAAAGGCTTTACCAGCAAACGCAAAGATCTCAAAAGAAGCAAAAGAAACAGTACAAGAGTGTGTATCAGAGTTTATCAGTTTCATAACAGGAGAAGCTTCTGATAAGTGTCAGAAAGAGAAGAGGAAAACAATCAACGGTGATGATCTTCTTTGGGCTATGACAACTCTTGGATTTGAAGATTATGTTGAGCCACTCAAGATTTATTTGAGTAAGTATAGAGAGATGGAAGGAGAGAAGAGTGCTATGATTGGTAGGAGTGATCAGAGAGATGGAAATGTTGGTGAGGGTAGTGGTGGAGTTTATGGTCATGGAGTTGGAGTTCCATCTTCTATGATGATGATGATGGGACATAATATGTATGGATCTGGGAGTGGATCGCCATCTTCTGGAAGGAGTACTAGATAG
Protein sequence

>MsaT007233.1

MVESDNESGGGQTGCREQDRFLPIANVSRIMKKALPANAKISKEAKETVQECVSEFISFITGEASDKCQKEKRKTINGDDLLWAMTTLGFEDYVEPLKIYLSKYREMEGEKSAMIGRSDQRDGNVGEGSGGVYGHGVGVPSSMMMMMGHNMYGSGSGSPSSGRSTR*