AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007315


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007315 MtrunA17_Chr2g0282771 97.345 113 2 1 1 112 1 113 8.58e-69 223
MsaG007315 MtrunA17_Chr4g0046591 74.699 83 19 1 26 108 18 98 3.40e-35 127
MsaG007315 MtrunA17_Chr4g0040511 69.841 63 19 0 36 98 16 78 3.49e-24 95.9
MsaG007315 MtrunA17_Chr5g0441991 51.724 58 28 0 41 98 20 77 1.71e-15 70.9
MsaG007315 MtrunA17_Chr3g0094681 50.909 55 27 0 42 96 21 75 2.69e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007315 AT1G28420.1 71.795 78 18 1 33 106 33 110 1.22e-29 111
MsaG007315 AT5G44180.2 64.286 70 24 1 37 105 15 84 1.38e-22 91.7
MsaG007315 AT5G44180.3 64.286 70 24 1 37 105 15 84 1.87e-22 91.3
MsaG007315 AT5G44180.1 64.286 70 24 1 37 105 15 84 1.87e-22 91.3
MsaG007315 AT4G03250.1 47.368 57 30 0 42 98 22 78 5.59e-14 67.0

Find 31 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGCCACCTTTGCTGGATTC+TGG 0.216776 2:+16432509 MsaT007315.1:CDS
GCGAAAGGCGCCACCTTTGC+TGG 0.311681 2:+16432502 MsaT007315.1:CDS
GCCTTTCGCGCCTTCTTAAC+CGG 0.347806 2:-16432488 None:intergenic
AACAACAAGATTATGAGTTC+AGG 0.378782 2:+16432166 MsaT007315.1:CDS
TCAAGCATTTCAAGCTGAAA+AGG 0.383787 2:-16432226 None:intergenic
CAAGCATTTCAAGCTGAAAA+GGG 0.399966 2:-16432225 None:intergenic
GTCTGAGAAGTTGGGGTTGT+CGG 0.401007 2:+16432409 MsaT007315.1:CDS
GATAGAGCTGTCTGAGAAGT+TGG 0.402849 2:+16432400 MsaT007315.1:CDS
CCTTCTTGTCTTTCAACCTC+CGG 0.426074 2:-16432460 None:intergenic
ATAGAGCTGTCTGAGAAGTT+GGG 0.440544 2:+16432401 MsaT007315.1:CDS
GAAAGACAAGAAGGAGTTAC+CGG 0.460260 2:+16432469 MsaT007315.1:CDS
AATTCCCAGAAGGGTGATGA+TGG 0.465278 2:+16432106 MsaT007315.1:CDS
TTCAGTGGAGACATATCCTT+CGG 0.476034 2:+16432367 MsaT007315.1:intron
CCGGATCCATATTCATTACA+AGG 0.514192 2:-16432534 None:intergenic
GAAGGAGTTACCGGTTAAGA+AGG 0.522295 2:+16432478 MsaT007315.1:CDS
ATTCCCAGAAGGGTGATGAT+GGG 0.552822 2:+16432107 MsaT007315.1:CDS
AGTAATGAAAATTTCAACAA+TGG 0.570399 2:+16432139 MsaT007315.1:CDS
TCTATCCTTGTTGTCTCCGA+AGG 0.573578 2:-16432383 None:intergenic
ACCGGTTAAGAAGGCGCGAA+AGG 0.574449 2:+16432487 MsaT007315.1:CDS
TTCCCAGAAGGGTGATGATG+GGG 0.582040 2:+16432108 MsaT007315.1:CDS
GCAGAGAATAATTCCCAGAA+GGG 0.596544 2:+16432097 MsaT007315.1:CDS
GCTCCAGAATCCAGCAAAGG+TGG 0.596648 2:-16432512 None:intergenic
CGGATCGACAGTTACAGATG+TGG 0.596696 2:+16432429 MsaT007315.1:CDS
TAGAGCTGTCTGAGAAGTTG+GGG 0.600237 2:+16432402 MsaT007315.1:CDS
AAGCATTTCAAGCTGAAAAG+GGG 0.602993 2:-16432224 None:intergenic
AGCAGAGAATAATTCCCAGA+AGG 0.615412 2:+16432096 MsaT007315.1:CDS
CATATCCTTCGGAGACAACA+AGG 0.621717 2:+16432378 MsaT007315.1:CDS
AAGATTATGAGTTCAGGTGA+AGG 0.636764 2:+16432172 MsaT007315.1:CDS
CCGGAGGTTGAAAGACAAGA+AGG 0.646614 2:+16432460 MsaT007315.1:CDS
TCCCAGAAGGGTGATGATGG+GGG 0.649850 2:+16432109 MsaT007315.1:CDS
AAGGCTCCAGAATCCAGCAA+AGG 0.677006 2:-16432515 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 16432085 16432541 16432085 ID=MsaG007315
Chr2 mRNA 16432085 16432541 16432085 ID=MsaT007315.1;Parent=MsaG007315
Chr2 exon 16432085 16432262 16432085 ID=MsaT007315.1.exon1;Parent=MsaT007315.1
Chr2 CDS 16432085 16432262 16432085 ID=cds.MsaT007315.1;Parent=MsaT007315.1
Chr2 exon 16432372 16432541 16432372 ID=MsaT007315.1.exon2;Parent=MsaT007315.1
Chr2 CDS 16432372 16432541 16432372 ID=cds.MsaT007315.1;Parent=MsaT007315.1
Gene Sequence

>MsaT007315.1

ATGGAAGCTGAAGCAGAGAATAATTCCCAGAAGGGTGATGATGGGGGTGAAAATAGTAATGAAAATTTCAACAATGGAAGTAACAACAAGATTATGAGTTCAGGTGAAGGTCAAAGCAAACCTAAGAGACAAATGAAAACCCCTTTTCAGCTTGAAATGCTTGAGAAAGCTTATGCTTTGGAGACATATCCTTCGGAGACAACAAGGATAGAGCTGTCTGAGAAGTTGGGGTTGTCGGATCGACAGTTACAGATGTGGTTTTGTCACCGGAGGTTGAAAGACAAGAAGGAGTTACCGGTTAAGAAGGCGCGAAAGGCGCCACCTTTGCTGGATTCTGGAGCCTTGTAA

Protein sequence

>MsaT007315.1

MEAEAENNSQKGDDGGENSNENFNNGSNNKIMSSGEGQSKPKRQMKTPFQLEMLEKAYALETYPSETTRIELSEKLGLSDRQLQMWFCHRRLKDKKELPVKKARKAPPLLDSGAL*