Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG007315 | XP_024631664.1 | 97.345 | 113 | 2 | 1 | 1 | 112 | 1 | 113 | 9.33e-65 | 223 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG007315 | sp|F4HY56|RLT1_ARATH | 71.795 | 78 | 18 | 1 | 33 | 106 | 33 | 110 | 1.20e-28 | 111 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG007315 | tr|A2Q504|A2Q504_MEDTR | 97.345 | 113 | 2 | 1 | 1 | 112 | 1 | 113 | 4.46e-65 | 223 |
Gene ID | Type | Classification |
---|---|---|
MsaG007315 | TF | HB-other |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000107 | MsaG007315 | 0.801094 | 1.773685e-48 | 2.458004e-46 |
MsaG000419 | MsaG007315 | 0.819509 | 1.956635e-52 | 4.247837e-50 |
MsaG002978 | MsaG007315 | 0.805450 | 2.244909e-49 | 3.439153e-47 |
MsaG004014 | MsaG007315 | 0.838428 | 5.321322e-57 | 1.977652e-54 |
MsaG004092 | MsaG007315 | 0.811300 | 1.286278e-50 | 2.267458e-48 |
MsaG004112 | MsaG007315 | 0.800921 | 1.923118e-48 | 2.654712e-46 |
MsaG004424 | MsaG007315 | 0.821407 | 7.212174e-53 | 1.646368e-50 |
MsaG004986 | MsaG007315 | 0.805651 | 2.038325e-49 | 3.137370e-47 |
MsaG005189 | MsaG007315 | 0.812281 | 7.885643e-51 | 1.423974e-48 |
MsaG005239 | MsaG007315 | 0.811079 | 1.435677e-50 | 2.517145e-48 |
MsaG005329 | MsaG007315 | 0.808744 | 4.540283e-50 | 7.522578e-48 |
MsaG005406 | MsaG007315 | 0.830894 | 4.090254e-55 | 1.214427e-52 |
MsaG005702 | MsaG007315 | 0.808719 | 4.596829e-50 | 7.611667e-48 |
MsaG005886 | MsaG007315 | 0.832509 | 1.642728e-55 | 5.111853e-53 |
MsaG005984 | MsaG007315 | 0.818295 | 3.683701e-52 | 7.747154e-50 |
MsaG006610 | MsaG007315 | 0.826711 | 4.161269e-54 | 1.097623e-51 |
MsaG006855 | MsaG007315 | 0.812030 | 8.938754e-51 | 1.604204e-48 |
MsaG007241 | MsaG007315 | 0.824687 | 1.249832e-53 | 3.117100e-51 |
MsaG007315 | MsaG007335 | 0.804615 | 3.348640e-49 | 5.031509e-47 |
MsaG007315 | MsaG007354 | 0.825406 | 8.471352e-54 | 2.154840e-51 |
MsaG007315 | MsaG008220 | 0.801329 | 1.588588e-48 | 2.213294e-46 |
MsaG007315 | MsaG008495 | 0.800859 | 1.979852e-48 | 2.729272e-46 |
MsaG007315 | MsaG008634 | 0.839294 | 3.184830e-57 | 1.215996e-54 |
MsaG007315 | MsaG009603 | 0.813217 | 4.929850e-51 | 9.112706e-49 |
MsaG007315 | MsaG010104 | 0.839703 | 2.495912e-57 | 9.653135e-55 |
MsaG007315 | MsaG010365 | 0.818630 | 3.094690e-52 | 6.565579e-50 |
MsaG007315 | MsaG010860 | 0.817399 | 5.855356e-52 | 1.203379e-49 |
MsaG007315 | MsaG011298 | 0.832521 | 1.631315e-55 | 5.078075e-53 |
MsaG007315 | MsaG012489 | 0.814906 | 2.098867e-51 | 4.047691e-49 |
MsaG007315 | MsaG015018 | 0.812530 | 6.958991e-51 | 1.264512e-48 |
MsaG007315 | MsaG017057 | 0.821662 | 6.302967e-53 | 1.448608e-50 |
MsaG007315 | MsaG017303 | 0.812075 | 8.739593e-51 | 1.570149e-48 |
MsaG007315 | MsaG017286 | 0.824808 | 1.170522e-53 | 2.929038e-51 |
MsaG007315 | MsaG017412 | 0.819488 | 1.979176e-52 | 4.294379e-50 |
MsaG007315 | MsaG018229 | 0.813123 | 5.167458e-51 | 9.529447e-49 |
MsaG007315 | MsaG018489 | 0.808765 | 4.495050e-50 | 7.451058e-48 |
MsaG007315 | MsaG018786 | 0.807903 | 6.849433e-50 | 1.112085e-47 |
MsaG007315 | MsaG018975 | 0.815929 | 1.246043e-51 | 2.466043e-49 |
MsaG007315 | MsaG019308 | 0.839515 | 2.792364e-57 | 1.073552e-54 |
MsaG007315 | MsaG019935 | 0.801643 | 1.370617e-48 | 1.923271e-46 |
MsaG007315 | MsaG020245 | 0.812651 | 6.551642e-51 | 1.194017e-48 |
MsaG007315 | MsaG020288 | 0.844817 | 1.119460e-58 | 5.099094e-56 |
MsaG007315 | MsaG020467 | 0.816128 | 1.125246e-51 | 2.238303e-49 |
MsaG007315 | MsaG020652 | 0.805437 | 2.258474e-49 | 3.458893e-47 |
MsaG007315 | MsaG021022 | 0.811110 | 1.413351e-50 | 2.479886e-48 |
MsaG007315 | MsaG022284 | 0.828774 | 1.335615e-54 | 3.732420e-52 |
MsaG007315 | MsaG025289 | 0.827808 | 2.278291e-54 | 6.196868e-52 |
MsaG007315 | MsaG026022 | 0.820885 | 9.502158e-53 | 2.139381e-50 |
MsaG007315 | MsaG026192 | 0.817334 | 6.055782e-52 | 1.242480e-49 |
MsaG007315 | MsaG026849 | 0.801604 | 1.395528e-48 | 1.956513e-46 |
MsaG007315 | MsaG026831 | 0.804220 | 4.044452e-49 | 6.021907e-47 |
MsaG007315 | MsaG026987 | 0.836707 | 1.462878e-56 | 5.157769e-54 |
MsaG007315 | MsaG027505 | 0.816431 | 9.634889e-52 | 1.931349e-49 |
MsaG007315 | MsaG028109 | 0.800190 | 2.705221e-48 | 3.673259e-46 |
MsaG007315 | MsaG029093 | 0.803134 | 6.779813e-49 | 9.843814e-47 |
MsaG007315 | MsaG029422 | 0.814427 | 2.675903e-51 | 5.098781e-49 |
MsaG007315 | MsaG033593 | 0.806016 | 1.709578e-49 | 2.653957e-47 |
MsaG007315 | MsaG034977 | 0.812636 | 6.598676e-51 | 1.202166e-48 |
MsaG007315 | MsaG035151 | 0.805600 | 2.088080e-49 | 3.210103e-47 |
MsaG007315 | MsaG035198 | 0.850870 | 2.449308e-60 | 1.367888e-57 |
MsaG007315 | MsaG035743 | 0.801557 | 1.427145e-48 | 1.998650e-46 |
MsaG007315 | MsaG042000 | 0.817586 | 5.317098e-52 | 1.097994e-49 |
MsaG007315 | MsaG042579 | 0.819333 | 2.145411e-52 | 4.636462e-50 |
MsaG007315 | MsaG043788 | 0.814641 | 2.401024e-51 | 4.599444e-49 |
MsaG007315 | MsaG044278 | 0.832157 | 2.005393e-55 | 6.177172e-53 |
MsaG007315 | MsaG044477 | 0.802416 | 9.523351e-49 | 1.360126e-46 |
MsaG007315 | MsaG044957 | 0.815474 | 1.571789e-51 | 3.075000e-49 |
MsaG007315 | MsaG045251 | 0.828918 | 1.233262e-54 | 3.460404e-52 |
MsaG007315 | MsaG045548 | 0.815339 | 1.683664e-51 | 3.282748e-49 |
MsaG007315 | MsaG045568 | 0.821693 | 6.201403e-53 | 1.426437e-50 |
MsaG007315 | MsaG045767 | 0.815963 | 1.224256e-51 | 2.425089e-49 |
MsaG007315 | MsaG045820 | 0.805864 | 1.839521e-49 | 2.845537e-47 |
MsaG007315 | MsaG046027 | 0.854972 | 1.665883e-61 | 1.075065e-58 |
MsaG007315 | MsaG046498 | 0.805728 | 1.963618e-49 | 3.027852e-47 |
MsaG007315 | MsaG046507 | 0.848424 | 1.171368e-59 | 6.016303e-57 |
MsaG007315 | MsaG047011 | 0.803991 | 4.511833e-49 | 6.681969e-47 |
MsaG007315 | MsaG004665 | 0.812698 | 6.397176e-51 | 1.167286e-48 |
MsaG007315 | MsaG001950 | 0.824737 | 1.216650e-53 | 3.038474e-51 |
MsaG007315 | MsaG009513 | 0.800297 | 2.573520e-48 | 3.502914e-46 |
MsaG007315 | MsaG012946 | 0.817316 | 6.112508e-52 | 1.253559e-49 |
MsaG007315 | MsaG011874 | 0.836677 | 1.488445e-56 | 5.242968e-54 |
MsaG007315 | MsaG013252 | 0.835618 | 2.756780e-56 | 9.406730e-54 |
MsaG007315 | MsaG019605 | 0.841173 | 1.034093e-57 | 4.188887e-55 |
MsaG007315 | MsaG019592 | 0.803218 | 6.514994e-49 | 9.477613e-47 |
MsaG007315 | MsaG020277 | 0.850244 | 3.664274e-60 | 2.002489e-57 |
MsaG007315 | MsaG019828 | 0.862146 | 1.232730e-63 | 1.040431e-60 |
MsaG007315 | MsaG028121 | 0.806879 | 1.126371e-49 | 1.784643e-47 |
MsaG007315 | MsaG030007 | 0.812713 | 6.349193e-51 | 1.158942e-48 |
MsaG007315 | MsaG034262 | 0.838898 | 4.029150e-57 | 1.519604e-54 |
MsaG007315 | MsaG030563 | 0.811822 | 9.917141e-51 | 1.770764e-48 |
MsaG007315 | MsaG036471 | 0.834683 | 4.734875e-56 | 1.571103e-53 |
MsaG007315 | MsaG036661 | 0.803296 | 6.276930e-49 | 9.147831e-47 |
MsaG007315 | MsaG037513 | 0.802208 | 1.050245e-48 | 1.492791e-46 |
MsaG007315 | MsaG036473 | 0.824979 | 1.067143e-53 | 2.682892e-51 |
MsaG007315 | MsaG037051 | 0.801330 | 1.587636e-48 | 2.212032e-46 |
MsaG007315 | MsaG035795 | 0.807598 | 7.943817e-50 | 1.280297e-47 |
MsaG007315 | MsaG041852 | 0.816292 | 1.034792e-51 | 2.067000e-49 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG007315 | MtrunA17_Chr2g0282771 | 97.345 | 113 | 2 | 1 | 1 | 112 | 1 | 113 | 8.58e-69 | 223 |
MsaG007315 | MtrunA17_Chr4g0046591 | 74.699 | 83 | 19 | 1 | 26 | 108 | 18 | 98 | 3.40e-35 | 127 |
MsaG007315 | MtrunA17_Chr4g0040511 | 69.841 | 63 | 19 | 0 | 36 | 98 | 16 | 78 | 3.49e-24 | 95.9 |
MsaG007315 | MtrunA17_Chr5g0441991 | 51.724 | 58 | 28 | 0 | 41 | 98 | 20 | 77 | 1.71e-15 | 70.9 |
MsaG007315 | MtrunA17_Chr3g0094681 | 50.909 | 55 | 27 | 0 | 42 | 96 | 21 | 75 | 2.69e-14 | 67.4 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG007315 | AT1G28420.1 | 71.795 | 78 | 18 | 1 | 33 | 106 | 33 | 110 | 1.22e-29 | 111 |
MsaG007315 | AT5G44180.2 | 64.286 | 70 | 24 | 1 | 37 | 105 | 15 | 84 | 1.38e-22 | 91.7 |
MsaG007315 | AT5G44180.3 | 64.286 | 70 | 24 | 1 | 37 | 105 | 15 | 84 | 1.87e-22 | 91.3 |
MsaG007315 | AT5G44180.1 | 64.286 | 70 | 24 | 1 | 37 | 105 | 15 | 84 | 1.87e-22 | 91.3 |
MsaG007315 | AT4G03250.1 | 47.368 | 57 | 30 | 0 | 42 | 98 | 22 | 78 | 5.59e-14 | 67.0 |
Find 31 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
GCGCCACCTTTGCTGGATTC+TGG | 0.216776 | 2:+16432509 | MsaT007315.1:CDS |
GCGAAAGGCGCCACCTTTGC+TGG | 0.311681 | 2:+16432502 | MsaT007315.1:CDS |
GCCTTTCGCGCCTTCTTAAC+CGG | 0.347806 | 2:-16432488 | None:intergenic |
AACAACAAGATTATGAGTTC+AGG | 0.378782 | 2:+16432166 | MsaT007315.1:CDS |
TCAAGCATTTCAAGCTGAAA+AGG | 0.383787 | 2:-16432226 | None:intergenic |
CAAGCATTTCAAGCTGAAAA+GGG | 0.399966 | 2:-16432225 | None:intergenic |
GTCTGAGAAGTTGGGGTTGT+CGG | 0.401007 | 2:+16432409 | MsaT007315.1:CDS |
GATAGAGCTGTCTGAGAAGT+TGG | 0.402849 | 2:+16432400 | MsaT007315.1:CDS |
CCTTCTTGTCTTTCAACCTC+CGG | 0.426074 | 2:-16432460 | None:intergenic |
ATAGAGCTGTCTGAGAAGTT+GGG | 0.440544 | 2:+16432401 | MsaT007315.1:CDS |
GAAAGACAAGAAGGAGTTAC+CGG | 0.460260 | 2:+16432469 | MsaT007315.1:CDS |
AATTCCCAGAAGGGTGATGA+TGG | 0.465278 | 2:+16432106 | MsaT007315.1:CDS |
TTCAGTGGAGACATATCCTT+CGG | 0.476034 | 2:+16432367 | MsaT007315.1:intron |
CCGGATCCATATTCATTACA+AGG | 0.514192 | 2:-16432534 | None:intergenic |
GAAGGAGTTACCGGTTAAGA+AGG | 0.522295 | 2:+16432478 | MsaT007315.1:CDS |
ATTCCCAGAAGGGTGATGAT+GGG | 0.552822 | 2:+16432107 | MsaT007315.1:CDS |
AGTAATGAAAATTTCAACAA+TGG | 0.570399 | 2:+16432139 | MsaT007315.1:CDS |
TCTATCCTTGTTGTCTCCGA+AGG | 0.573578 | 2:-16432383 | None:intergenic |
ACCGGTTAAGAAGGCGCGAA+AGG | 0.574449 | 2:+16432487 | MsaT007315.1:CDS |
TTCCCAGAAGGGTGATGATG+GGG | 0.582040 | 2:+16432108 | MsaT007315.1:CDS |
GCAGAGAATAATTCCCAGAA+GGG | 0.596544 | 2:+16432097 | MsaT007315.1:CDS |
GCTCCAGAATCCAGCAAAGG+TGG | 0.596648 | 2:-16432512 | None:intergenic |
CGGATCGACAGTTACAGATG+TGG | 0.596696 | 2:+16432429 | MsaT007315.1:CDS |
TAGAGCTGTCTGAGAAGTTG+GGG | 0.600237 | 2:+16432402 | MsaT007315.1:CDS |
AAGCATTTCAAGCTGAAAAG+GGG | 0.602993 | 2:-16432224 | None:intergenic |
AGCAGAGAATAATTCCCAGA+AGG | 0.615412 | 2:+16432096 | MsaT007315.1:CDS |
CATATCCTTCGGAGACAACA+AGG | 0.621717 | 2:+16432378 | MsaT007315.1:CDS |
AAGATTATGAGTTCAGGTGA+AGG | 0.636764 | 2:+16432172 | MsaT007315.1:CDS |
CCGGAGGTTGAAAGACAAGA+AGG | 0.646614 | 2:+16432460 | MsaT007315.1:CDS |
TCCCAGAAGGGTGATGATGG+GGG | 0.649850 | 2:+16432109 | MsaT007315.1:CDS |
AAGGCTCCAGAATCCAGCAA+AGG | 0.677006 | 2:-16432515 | None:intergenic |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr2 | gene | 16432085 | 16432541 | 16432085 | ID=MsaG007315 |
Chr2 | mRNA | 16432085 | 16432541 | 16432085 | ID=MsaT007315.1;Parent=MsaG007315 |
Chr2 | exon | 16432085 | 16432262 | 16432085 | ID=MsaT007315.1.exon1;Parent=MsaT007315.1 |
Chr2 | CDS | 16432085 | 16432262 | 16432085 | ID=cds.MsaT007315.1;Parent=MsaT007315.1 |
Chr2 | exon | 16432372 | 16432541 | 16432372 | ID=MsaT007315.1.exon2;Parent=MsaT007315.1 |
Chr2 | CDS | 16432372 | 16432541 | 16432372 | ID=cds.MsaT007315.1;Parent=MsaT007315.1 |
Gene Sequence |
Protein sequence |