AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007323

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007323 MtrunA17_Chr2g0291581 89.124 662 56 4 1 655 35 687 0.0 1214
MsaG007323 MtrunA17_Chr2g0291581 47.826 184 86 2 707 890 55 228 8.26e-45 173
MsaG007323 MtrunA17_Chr2g0291581 44.144 111 56 2 894 1003 483 588 6.73e-17 85.9
MsaG007323 MtrunA17_Chr2g0291591 48.257 545 261 3 1 530 25 563 6.70e-176 525
MsaG007323 MtrunA17_Chr2g0291591 84.416 231 14 1 660 890 23 231 1.05e-124 392
MsaG007323 MtrunA17_Chr2g0291591 93.827 81 5 0 891 971 483 563 9.58e-40 156
MsaG007323 MtrunA17_Chr2g0296781 36.542 561 332 9 50 590 1 557 3.03e-115 372
MsaG007323 MtrunA17_Chr5g0441581 35.313 623 362 10 46 646 32 635 7.22e-115 370
MsaG007323 MtrunA17_Chr1g0207551 35.101 641 381 11 38 657 27 653 9.24e-112 365
MsaG007323 MtrunA17_Chr1g0207551 27.811 169 120 1 722 888 33 201 2.24e-11 68.2
MsaG007323 MtrunA17_Chr6g0477261 41.163 430 248 4 164 590 19 446 4.20e-110 356
MsaG007323 MtrunA17_Chr2g0329031 34.016 635 369 10 46 655 14 623 1.45e-109 357
MsaG007323 MtrunA17_Chr3g0080351 33.981 618 372 8 44 643 74 673 1.75e-105 349
MsaG007323 MtrunA17_Chr1g0196541 36.136 559 337 9 50 590 1 557 7.52e-103 338
MsaG007323 MtrunA17_Chr1g0202341 31.711 637 394 10 44 651 49 673 1.15e-102 337
MsaG007323 MtrunA17_Chr1g0202341 31.250 192 103 8 717 896 45 219 1.55e-13 75.1
MsaG007323 MtrunA17_Chr5g0448761 31.715 659 410 16 44 672 58 706 2.52e-93 314
MsaG007323 MtrunA17_Chr2g0287061 31.240 605 377 10 44 642 181 752 3.19e-84 290
MsaG007323 MtrunA17_Chr2g0287061 32.110 109 65 1 722 830 182 281 5.61e-11 67.0
MsaG007323 MtrunA17_Chr5g0440451 28.947 646 388 16 46 647 43 661 8.29e-82 286
MsaG007323 MtrunA17_Chr5g0448511 32.421 475 303 7 199 665 11 475 5.50e-79 268
MsaG007323 MtrunA17_Chr3g0118611 32.222 450 294 5 200 647 118 558 1.36e-77 270
MsaG007323 MtrunA17_Chr8g0363841 27.273 583 399 6 48 609 19 597 4.92e-63 227
MsaG007323 MtrunA17_Chr8g0363841 29.730 148 90 4 722 855 7 154 1.13e-11 68.9
MsaG007323 MtrunA17_Chr2g0324011 27.425 598 394 12 46 617 10 593 3.54e-62 223
MsaG007323 MtrunA17_Chr1g0203721 26.803 638 417 17 47 650 64 685 1.80e-58 217
MsaG007323 MtrunA17_Chr2g0287651 25.521 576 371 13 50 589 79 632 1.13e-53 202
MsaG007323 MtrunA17_Chr6g0458391 26.040 553 386 10 45 587 224 763 4.28e-53 199
MsaG007323 MtrunA17_Chr6g0458391 35.556 135 80 3 720 847 88 222 6.43e-11 66.6
MsaG007323 MtrunA17_Chr1g0198761 25.738 711 438 20 19 679 28 698 2.06e-52 197
MsaG007323 MtrunA17_Chr1g0203051 25.777 547 357 15 59 576 95 621 5.49e-50 188
MsaG007323 MtrunA17_Chr3g0082121 25.870 661 418 17 46 667 43 670 1.09e-47 182
MsaG007323 MtrunA17_Chr2g0276841 25.856 584 375 17 46 594 70 630 5.75e-47 180
MsaG007323 MtrunA17_Chr2g0294841 23.077 702 470 15 18 677 17 690 2.02e-38 154
MsaG007323 MtrunA17_Chr2g0290391 24.791 597 386 17 42 597 47 621 8.76e-38 152
MsaG007323 MtrunA17_Chr5g0431001 26.077 441 272 13 34 434 54 480 2.31e-33 135
MsaG007323 MtrunA17_Chr1g0164591 29.536 237 165 2 183 418 73 308 5.27e-28 116
MsaG007323 MtrunA17_Chr7g0258861 26.502 283 176 6 46 301 10 287 1.22e-22 100
MsaG007323 MtrunA17_Chr7g0258861 25.904 166 112 4 722 881 9 169 4.15e-11 65.5
MsaG007323 MtrunA17_Chr2g0308841 24.229 227 164 5 362 586 4 224 8.33e-19 87.8
MsaG007323 MtrunA17_Chr2g0329421 34.014 147 90 3 716 857 22 166 4.94e-15 75.1
MsaG007323 MtrunA17_Chr4g0033611 36.538 104 63 1 722 822 75 178 5.78e-15 76.3
MsaG007323 MtrunA17_Chr4g0033611 39.583 96 53 2 46 137 76 170 1.01e-11 66.6
MsaG007323 MtrunA17_Chr2g0291601 66.038 53 6 1 119 159 1 53 1.95e-13 65.9
MsaG007323 MtrunA17_Chr2g0329361 29.032 155 94 3 672 820 20 164 1.57e-12 68.9
MsaG007323 MtrunA17_Chr2g0329361 34.314 102 57 2 44 137 59 158 8.90e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007323 AT1G52520.1 61.162 654 226 10 21 655 59 703 0.0 828
MsaG007323 AT1G52520.1 48.756 201 81 6 686 878 42 228 3.69e-40 159
MsaG007323 AT1G52520.1 42.478 113 58 3 893 1003 498 605 3.53e-18 90.5
MsaG007323 AT1G80010.1 44.733 655 325 11 29 653 76 723 0.0 555
MsaG007323 AT1G80010.1 55.612 196 79 2 702 889 74 269 7.70e-58 213
MsaG007323 AT1G80010.1 44.231 104 50 2 894 997 525 620 1.25e-14 79.0
MsaG007323 AT4G15090.1 33.129 652 405 9 25 655 30 671 6.76e-115 374
MsaG007323 AT4G15090.5 33.129 652 405 9 25 655 30 671 6.76e-115 374
MsaG007323 AT4G15090.3 33.129 652 405 9 25 655 30 671 6.76e-115 374
MsaG007323 AT4G15090.2 33.129 652 405 9 25 655 30 671 6.76e-115 374
MsaG007323 AT4G15090.4 33.129 652 405 9 25 655 30 671 6.76e-115 374
MsaG007323 AT3G22170.2 34.161 644 368 11 25 644 57 668 2.26e-114 373
MsaG007323 AT3G22170.1 34.161 644 368 11 25 644 57 668 2.26e-114 373
MsaG007323 AT1G76320.3 34.395 628 350 12 50 646 1 597 1.49e-102 336
MsaG007323 AT1G76320.1 34.236 628 351 11 50 646 1 597 2.55e-102 338
MsaG007323 AT1G76320.4 34.236 628 351 11 50 646 1 597 2.55e-102 338
MsaG007323 AT1G76320.2 34.236 628 351 11 50 646 1 597 2.89e-102 337
MsaG007323 AT4G19990.3 34.629 566 315 9 49 589 24 559 6.69e-91 307
MsaG007323 AT4G19990.4 34.629 566 315 9 49 589 24 559 6.69e-91 307
MsaG007323 AT4G19990.5 34.629 566 315 9 49 589 24 559 9.81e-91 306
MsaG007323 AT4G19990.6 34.629 566 315 9 49 589 24 559 1.28e-90 307
MsaG007323 AT4G19990.2 34.629 566 315 9 49 589 24 559 1.28e-90 307
MsaG007323 AT4G19990.7 34.629 566 315 9 49 589 38 573 1.40e-90 306
MsaG007323 AT2G27110.2 29.921 635 399 14 32 653 40 641 1.48e-87 300
MsaG007323 AT2G27110.1 29.921 635 399 14 32 653 40 641 1.48e-87 300
MsaG007323 AT4G38180.1 31.414 573 361 10 46 593 73 638 4.04e-87 298
MsaG007323 AT4G38180.1 33.077 130 79 2 722 843 72 201 5.54e-11 67.4
MsaG007323 AT3G06250.2 29.853 613 397 10 32 643 176 756 4.71e-85 291
MsaG007323 AT3G06250.2 30.405 148 91 3 722 869 27 162 1.04e-12 72.8
MsaG007323 AT3G06250.1 29.853 613 397 10 32 643 176 756 4.71e-85 291
MsaG007323 AT3G06250.1 30.405 148 91 3 722 869 27 162 1.04e-12 72.8
MsaG007323 AT3G06250.3 29.853 613 397 10 32 643 176 756 4.71e-85 291
MsaG007323 AT3G06250.3 30.405 148 91 3 722 869 27 162 1.04e-12 72.8
MsaG007323 AT4G19990.1 35.656 488 278 6 109 589 44 502 2.38e-84 288
MsaG007323 AT5G18960.2 30.413 605 379 14 46 643 211 780 2.13e-82 285
MsaG007323 AT5G18960.2 28.889 135 87 1 721 855 41 166 1.39e-12 72.4
MsaG007323 AT5G18960.1 30.413 605 379 14 46 643 211 780 2.13e-82 285
MsaG007323 AT5G18960.1 28.889 135 87 1 721 855 41 166 1.39e-12 72.4
MsaG007323 AT2G27110.3 31.942 479 316 7 176 653 27 496 6.07e-82 281
MsaG007323 AT2G32250.5 32.137 613 350 14 49 644 41 604 1.13e-81 282
MsaG007323 AT2G32250.2 33.151 549 317 10 49 586 41 550 1.19e-81 283
MsaG007323 AT2G32250.4 33.151 549 317 10 49 586 41 550 1.19e-81 283
MsaG007323 AT2G32250.6 32.137 613 350 14 49 644 41 604 1.21e-81 283
MsaG007323 AT2G32250.1 32.137 613 350 14 49 644 41 604 1.21e-81 283
MsaG007323 AT2G32250.3 32.137 613 350 14 49 644 41 604 1.21e-81 283
MsaG007323 AT2G32250.7 32.847 548 318 10 50 586 1 509 6.67e-81 280
MsaG007323 AT2G32250.8 31.863 612 351 14 50 644 1 563 7.01e-81 280
MsaG007323 AT4G38170.1 32.018 456 296 8 204 656 21 465 6.30e-72 249
MsaG007323 AT1G10240.2 26.117 582 364 15 46 586 48 604 8.06e-48 182
MsaG007323 AT1G10240.1 26.117 582 364 15 46 586 48 604 8.06e-48 182
MsaG007323 AT5G28530.1 23.920 648 426 15 45 650 54 676 1.14e-34 142
MsaG007323 AT5G28530.3 24.037 649 420 16 45 650 54 672 2.10e-34 141
MsaG007323 AT5G28530.2 24.037 649 420 16 45 650 54 672 2.10e-34 141
MsaG007323 AT5G28530.4 23.349 621 400 19 45 650 54 613 5.10e-27 118
MsaG007323 AT5G28530.5 23.473 622 394 20 45 650 54 609 7.70e-27 117
MsaG007323 AT3G59470.3 37.037 108 65 1 722 826 69 176 6.56e-15 76.3
MsaG007323 AT3G59470.3 39.130 92 51 2 46 133 70 160 7.41e-11 64.3
MsaG007323 AT3G59470.1 37.037 108 65 1 722 826 69 176 6.56e-15 76.3
MsaG007323 AT3G59470.1 39.130 92 51 2 46 133 70 160 7.41e-11 64.3
MsaG007323 AT3G59470.2 37.037 108 65 1 722 826 81 188 7.64e-15 76.3
MsaG007323 AT3G59470.2 39.130 92 51 2 46 133 82 172 9.16e-11 64.3

Find 193 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCAACTTATTAGAATATT+TGG 0.117558 2:-16480862 None:intergenic
TTATCTTGACAACACTTATT+TGG 0.170954 2:+16481052 MsaT007323.1:CDS
GAAGAAATGTTCTCTTGTTT+TGG 0.204791 2:+16481794 MsaT007323.1:CDS
TGCACGAAAGCACTTATTAA+TGG 0.206460 2:-16489334 None:intergenic
AATAGCTATGCCAGGGAAAT+TGG 0.208039 2:+16486942 MsaT007323.1:CDS
TCAATGATGAAGGGCATTTA+AGG 0.212565 2:+16480970 MsaT007323.1:CDS
GGCATTTAAGGAATGCATTA+TGG 0.227561 2:+16480982 MsaT007323.1:CDS
AACTGAAGCTCTTGATGATT+TGG 0.234723 2:+16488168 MsaT007323.1:CDS
CATGGTCAATCAGTGTTATT+AGG 0.249432 2:+16481122 MsaT007323.1:CDS
GGGAAATTGGATTTGCTATT+CGG 0.274552 2:+16486955 MsaT007323.1:CDS
GGATTCATGATTCAATGTTT+TGG 0.288413 2:+16481437 MsaT007323.1:CDS
AAATAATAGAATCATATAAA+TGG 0.289585 2:+16481165 MsaT007323.1:CDS
TTAGGTTGTGGCCTGCTTGC+AGG 0.291307 2:+16481140 MsaT007323.1:CDS
AAATGCCTATCATTTCTTAC+TGG 0.295826 2:-16488385 None:intergenic
TCCATCAAATAAAAGAAGTT+TGG 0.301368 2:-16480945 None:intergenic
CGATATTTCTGAGGGTAAAA+AGG 0.306656 2:+16480367 MsaT007323.1:CDS
GGTATGTCTGCAACACGGTC+TGG 0.311372 2:+16481545 MsaT007323.1:CDS
AGTCGGCTCAACCTTTCTTT+TGG 0.323275 2:-16487237 None:intergenic
CTGCAGTTCAAAAGAGCATT+TGG 0.324605 2:-16481720 None:intergenic
CGAGAAACAAGCGGAAATTA+AGG 0.328330 2:+16481877 MsaT007323.1:CDS
TGGCCCTATCACCGTCTTCT+TGG 0.333592 2:+16481838 MsaT007323.1:CDS
ACTGTAGATGCTTCCGGTTA+TGG 0.346656 2:+16487302 MsaT007323.1:CDS
CTGTAGATGCTTCCGGTTAT+GGG 0.351570 2:+16487303 MsaT007323.1:CDS
TTGACCAAGAAGACGGTGAT+AGG 0.355516 2:-16481842 None:intergenic
TCATTACAAGGTAGCGTTGC+AGG 0.357719 2:+16482192 MsaT007323.1:CDS
GCTAAAGCAATGATGAGATC+TGG 0.357977 2:-16481288 None:intergenic
CATGGCAGGACAACCAGTTC+TGG 0.364832 2:-16480600 None:intergenic
GATGGTGGCGAACATGAAAA+TGG 0.368179 2:+16486801 MsaT007323.1:CDS
ATCCCTCTTGTGGCTTTAGT+TGG 0.368401 2:+16481089 MsaT007323.1:CDS
GCATTTAAGGAATGCATTAT+GGG 0.369143 2:+16480983 MsaT007323.1:CDS
TGCAGAGGGGAAAACAATTA+AGG 0.370228 2:+16480764 MsaT007323.1:CDS
ACGGCAGAGGAAATTATAAA+TGG 0.374105 2:-16480907 None:intergenic
CACACAAAATCCAACCTCTT+TGG 0.375093 2:-16480462 None:intergenic
TATGGGTTGATGCTAAATCT+AGG 0.378405 2:+16481000 MsaT007323.1:CDS
GTGTGAGGATTAAGAATTCA+TGG 0.378670 2:+16480480 MsaT007323.1:CDS
CTTCTCTTTACTGTTACGTT+TGG 0.380146 2:-16486994 None:intergenic
CACATCCATGATTACTACTA+TGG 0.380508 2:+16489301 MsaT007323.1:CDS
GTATGTCTGCAACACGGTCT+GGG 0.384016 2:+16481546 MsaT007323.1:CDS
ACATGGAACACATGTATATC+AGG 0.387568 2:+16481197 MsaT007323.1:CDS
GGAAATTGGATTTGCTATTC+GGG 0.388562 2:+16486956 MsaT007323.1:CDS
TGAGAGTAGTGGAATCTAAT+AGG 0.393466 2:+16487120 MsaT007323.1:CDS
TTTACGAAGACCGAGTTCAT+TGG 0.400562 2:+16481489 MsaT007323.1:CDS
TCAAACTCCACTCCCTCAAC+AGG 0.406663 2:-16486891 None:intergenic
AGAATTTGAGCTCCCATAAC+CGG 0.414377 2:-16487315 None:intergenic
AACCGATTAGACCATTGAAT+AGG 0.415320 2:-16482121 None:intergenic
ACCCTATTCAATGGTCTAAT+CGG 0.415941 2:+16482119 MsaT007323.1:CDS
CCACAAACAGACTTCTTTGA+AGG 0.418328 2:+16481601 MsaT007323.1:CDS
TTCCACTTCAAGTTGGAACT+TGG 0.419493 2:-16481774 None:intergenic
AGAGCACCATCTTCATCTCT+TGG 0.423726 2:-16480323 None:intergenic
ACCGATTAGACCATTGAATA+GGG 0.424191 2:-16482120 None:intergenic
CCTTCAAAGAAGTCTGTTTG+TGG 0.428381 2:-16481601 None:intergenic
TTGATGGATTTCAATGATGA+AGG 0.428882 2:+16480960 MsaT007323.1:CDS
ATGCCAACTGTAGATGCTTC+CGG 0.429407 2:+16487296 MsaT007323.1:CDS
AATTTGTGAGACGCTGAAAC+AGG 0.436325 2:-16488308 None:intergenic
ATTTCAATGCATTCTCCATC+CGG 0.439531 2:-16480299 None:intergenic
CTCTTTCAGGATCAAATGAA+TGG 0.442050 2:-16487175 None:intergenic
CTCCACTCCCTCAACAGGTT+GGG 0.449152 2:-16486886 None:intergenic
AAGAACCAGTAAGAAATGAT+AGG 0.455809 2:+16488380 MsaT007323.1:CDS
TCCCTCCTCCACAACTTGTA+AGG 0.455897 2:-16482155 None:intergenic
CAATCAGTGTTATTAGGTTG+TGG 0.457165 2:+16481128 MsaT007323.1:CDS
TTACGAAGACCGAGTTCATT+GGG 0.459257 2:+16481490 MsaT007323.1:CDS
CATGATAAGGATTAAGTTAG+TGG 0.463158 2:+16480619 MsaT007323.1:CDS
TTCCCCTCTGCATCAGAACT+AGG 0.470411 2:-16480753 None:intergenic
CAACACCATAGTAGTAATCA+TGG 0.474207 2:-16489306 None:intergenic
TAGTGGAATCTAATAGGTGG+AGG 0.476847 2:+16487126 MsaT007323.1:CDS
ATTTGCTATGCCAAAGAGGT+TGG 0.481359 2:+16480452 MsaT007323.1:CDS
TCGTGATGTAGAACACAAAC+AGG 0.481522 2:+16482240 MsaT007323.1:CDS
TTGAGAAAAGAAACCAGAAC+TGG 0.483081 2:+16480587 MsaT007323.1:CDS
CTGGTTGTCCTGCCATGATA+AGG 0.483669 2:+16480606 MsaT007323.1:CDS
ATAGCTATTATAATAGTTGT+AGG 0.483777 2:-16486928 None:intergenic
CTATTCGGGTTAAATCTTCG+TGG 0.484193 2:+16486970 MsaT007323.1:CDS
CGCCCAACCTGTTGAGGGAG+TGG 0.485119 2:+16486884 MsaT007323.1:CDS
TAGAAGTGCCTTACAAGTTG+TGG 0.488022 2:+16482147 MsaT007323.1:CDS
ACATCATCACCACCTCATCT+TGG 0.493280 2:-16488282 None:intergenic
GTGCTTTGTTGTAGCAGTCA+AGG 0.493576 2:+16480536 MsaT007323.1:CDS
AGCTCTGTCATCTCTTGCAT+TGG 0.494419 2:-16480835 None:intergenic
CTAAAGCAATGATGAGATCT+GGG 0.494575 2:-16481287 None:intergenic
GTCTTGTGTTGTAGTTGTGA+AGG 0.496440 2:+16487026 MsaT007323.1:CDS
CACCAAGTTCCAACTTGAAG+TGG 0.497024 2:+16481772 MsaT007323.1:CDS
TAGAGAGTTGCAACTCATAG+TGG 0.499235 2:-16488228 None:intergenic
CGTAGGAAAGAAACAAGAAC+AGG 0.499540 2:+16487077 MsaT007323.1:CDS
CTCCTAAGTACATTCTTTCG+CGG 0.499725 2:+16482026 MsaT007323.1:CDS
ACTATTATAATAGCTATGCC+AGG 0.505200 2:+16486934 MsaT007323.1:CDS
GGAGGAGGGAGTAATTTCTC+TGG 0.511124 2:+16482168 MsaT007323.1:CDS
AGGACTAAAACGGTTGTGAC+CGG 0.512191 2:-16482080 None:intergenic
CACATAGGAGGATGGACAGT+GGG 0.512891 2:+16487213 MsaT007323.1:CDS
CTATTATTTCACCTGCAAGC+AGG 0.514073 2:-16481151 None:intergenic
AACTTGTCCTTCAGATATAC+CGG 0.514192 2:-16481515 None:intergenic
TGCCTTACAAGTTGTGGAGG+AGG 0.514813 2:+16482153 MsaT007323.1:CDS
AATAAGTTGAACCTAAGAAA+AGG 0.515686 2:+16480873 MsaT007323.1:CDS
ACATTTCTTCCACTTCAAGT+TGG 0.516480 2:-16481781 None:intergenic
AACTTAATCCTTATCATGGC+AGG 0.517948 2:-16480614 None:intergenic
TGATGGATTTCAATGATGAA+GGG 0.520805 2:+16480961 MsaT007323.1:CDS
CAGTATCATCAGGACTAAAA+CGG 0.522367 2:-16482090 None:intergenic
TACTATTTCCAGATAGATGA+GGG 0.522528 2:+16486696 MsaT007323.1:intron
GGGTCAGTATCAGTATCATC+AGG 0.522563 2:-16482100 None:intergenic
AATTTCTCTGGATCATTACA+AGG 0.524035 2:+16482180 MsaT007323.1:CDS
TAAGCAACTACTTGAGGTTG+AGG 0.526880 2:+16486731 MsaT007323.1:CDS
CTGATACTGACCCTATTCAA+TGG 0.530519 2:+16482110 MsaT007323.1:CDS
GGACAGTGGGGCCAAAAGAA+AGG 0.531952 2:+16487226 MsaT007323.1:CDS
CTATCTGTAATAATAGTTTG+AGG 0.532529 2:-16481227 None:intergenic
TCAAACTCCATTCCGACGGC+AGG 0.533529 2:-16480401 None:intergenic
GGGAGCTCAAATTCTAATGA+AGG 0.533763 2:+16487323 MsaT007323.1:CDS
CACTAACTTAATCCTTATCA+TGG 0.537066 2:-16480618 None:intergenic
AGACTACGCCCAACCTGTTG+AGG 0.540785 2:+16486878 MsaT007323.1:CDS
TAGTCAATTGCATACGGCAG+AGG 0.541377 2:-16480920 None:intergenic
TTGGATTAGTCAATTGCATA+CGG 0.547484 2:-16480926 None:intergenic
ACATAGTGGCGGTAACACTT+TGG 0.550501 2:+16482442 MsaT007323.1:intron
ACTCCACTCCCTCAACAGGT+TGG 0.553844 2:-16486887 None:intergenic
GATTGAATTCGATGCAGCAT+GGG 0.556013 2:+16481415 MsaT007323.1:CDS
GACTACGCCCAACCTGTTGA+GGG 0.556672 2:+16486879 MsaT007323.1:CDS
ATGGGTTGATGCTAAATCTA+GGG 0.557484 2:+16481001 MsaT007323.1:CDS
TGATTGAATTCGATGCAGCA+TGG 0.558452 2:+16481414 MsaT007323.1:CDS
TGTTGCACCTGCCGTCGGAA+TGG 0.558619 2:+16480394 MsaT007323.1:CDS
AATAGCAAATCCAATTTCCC+TGG 0.562021 2:-16486952 None:intergenic
CTGATTCTTAGCTCGGAAGA+AGG 0.563727 2:+16489260 MsaT007323.1:intron
TGACCAAGAAGACGGTGATA+GGG 0.563728 2:-16481841 None:intergenic
ACAATGCAATTACACGTGGA+TGG 0.565499 2:+16481818 MsaT007323.1:CDS
ACATGGTAAAAGAACATCAA+GGG 0.568150 2:+16488356 MsaT007323.1:CDS
TGCAGCAAGCAATCGCCGGA+TGG 0.569478 2:+16480284 MsaT007323.1:CDS
GAATTTGTTGCACCTGCCGT+CGG 0.571617 2:+16480389 MsaT007323.1:CDS
GCCTTACAAGTTGTGGAGGA+GGG 0.572299 2:+16482154 MsaT007323.1:CDS
TTATATTTGCTATGCCAAAG+AGG 0.572634 2:+16480448 MsaT007323.1:CDS
AGTTAGTGGAGTCTCAGAGA+TGG 0.573080 2:+16480633 MsaT007323.1:CDS
CGAGTCTATAGTGACATACA+TGG 0.575473 2:+16488339 MsaT007323.1:CDS
AGCTCTTTACAAGAAGTACA+AGG 0.577775 2:+16481646 MsaT007323.1:CDS
CTCTAAATCACATAGGAGGA+TGG 0.578313 2:+16487205 MsaT007323.1:CDS
CAAATATGAAATCCCTCTTG+TGG 0.578562 2:+16481079 MsaT007323.1:CDS
TATCACCGTCTTCTTGGTCA+AGG 0.580733 2:+16481844 MsaT007323.1:CDS
GGACTAAAACGGTTGTGACC+GGG 0.581921 2:-16482079 None:intergenic
TTACTATTTCCAGATAGATG+AGG 0.583204 2:+16486695 MsaT007323.1:intron
ACCATGCAGCAAGCAATCGC+CGG 0.584825 2:+16480280 MsaT007323.1:CDS
GTGGCGAACATGAAAATGGA+AGG 0.585092 2:+16486805 MsaT007323.1:CDS
TTGTCAAGACGATATTTCTG+AGG 0.585249 2:+16480358 MsaT007323.1:CDS
ACTACTTGAGGTTGAGGACA+AGG 0.585298 2:+16486737 MsaT007323.1:CDS
ACAAAGTAAGCAACTACTTG+AGG 0.589391 2:+16486725 MsaT007323.1:CDS
TAGTGGAGTCTCAGAGATGG+AGG 0.590281 2:+16480636 MsaT007323.1:CDS
TTGCCTAGTTCTGATGCAGA+GGG 0.592446 2:+16480750 MsaT007323.1:CDS
ATACCGGTGCCCAATGAACT+CGG 0.592551 2:-16481499 None:intergenic
GAAATTCCAAGAGATGAAGA+TGG 0.592964 2:+16480317 MsaT007323.1:CDS
ATAAAGAAGCTAATAGTCGT+AGG 0.594056 2:+16487060 MsaT007323.1:CDS
AGTCAATTGTTTGAGATCGA+TGG 0.594570 2:+16486783 MsaT007323.1:CDS
CTAAGTACATTCTTTCGCGG+TGG 0.595177 2:+16482029 MsaT007323.1:CDS
TACATGGTAAAAGAACATCA+AGG 0.596297 2:+16488355 MsaT007323.1:CDS
CGCGAAAGAATGTACTTAGG+AGG 0.597604 2:-16482025 None:intergenic
AAAACTCTAAATCACATAGG+AGG 0.599156 2:+16487201 MsaT007323.1:CDS
CGACTCTAGATGTTGAAGTA+CGG 0.600367 2:+16487255 MsaT007323.1:CDS
ATAAATGGTTGTTTAGAACA+TGG 0.600697 2:+16481180 MsaT007323.1:CDS
GGAATCTAATAGGTGGAGGG+TGG 0.600823 2:+16487130 MsaT007323.1:CDS
TCCGGCGATTGCTTGCTGCA+TGG 0.603156 2:-16480281 None:intergenic
CAATTGTTTGAGATCGATGG+TGG 0.603449 2:+16486786 MsaT007323.1:CDS
ATTCAATAAGTTCCAAGATG+AGG 0.606495 2:+16488270 MsaT007323.1:CDS
ATTCCAACTAAAGCCACAAG+AGG 0.609409 2:-16481092 None:intergenic
TAACCGGAAGCATCTACAGT+TGG 0.612183 2:-16487299 None:intergenic
AAGTGCCTTACAAGTTGTGG+AGG 0.614403 2:+16482150 MsaT007323.1:CDS
TTGGGCACCGGTATATCTGA+AGG 0.614520 2:+16481508 MsaT007323.1:CDS
TGTCAAGACGATATTTCTGA+GGG 0.614817 2:+16480359 MsaT007323.1:CDS
ACTATGACTGTCAACTCCCG+TGG 0.616006 2:-16486835 None:intergenic
GAGTAGTGGAATCTAATAGG+TGG 0.616272 2:+16487123 MsaT007323.1:CDS
CAATAAGTTCCAAGATGAGG+TGG 0.616780 2:+16488273 MsaT007323.1:CDS
TTCCAACTAAAGCCACAAGA+GGG 0.616806 2:-16481091 None:intergenic
AGCTCAAATTCTAATGAAGG+AGG 0.617343 2:+16487326 MsaT007323.1:CDS
TATGTCTGCAACACGGTCTG+GGG 0.623170 2:+16481547 MsaT007323.1:CDS
CGCAAAACTCTAAATCACAT+AGG 0.623536 2:+16487198 MsaT007323.1:CDS
TTGCTGGTATGTCTGCAACA+CGG 0.624394 2:+16481540 MsaT007323.1:CDS
AATGGAAGGGAGGAAACCAC+GGG 0.626247 2:+16486819 MsaT007323.1:CDS
GTTGCCTAGTTCTGATGCAG+AGG 0.630104 2:+16480749 MsaT007323.1:CDS
CTAATAACACTGATTGACCA+TGG 0.632937 2:-16481121 None:intergenic
ACGCTCCTTGACCAAGAAGA+CGG 0.634531 2:-16481849 None:intergenic
AAATGGAAGGGAGGAAACCA+CGG 0.638573 2:+16486818 MsaT007323.1:CDS
AGACCGAGTTCATTGGGCAC+CGG 0.643751 2:+16481496 MsaT007323.1:CDS
CATGGTAAAAGAACATCAAG+GGG 0.645016 2:+16488357 MsaT007323.1:CDS
ATTGAATTCGATGCAGCATG+GGG 0.645271 2:+16481416 MsaT007323.1:CDS
CACCGCGAAAGAATGTACTT+AGG 0.647338 2:-16482028 None:intergenic
CGTACTTCAACATCTAGAGT+CGG 0.648024 2:-16487254 None:intergenic
CTATTATAATAGCTATGCCA+GGG 0.648310 2:+16486935 MsaT007323.1:CDS
TGGGACAATGCAATTACACG+TGG 0.650233 2:+16481814 MsaT007323.1:CDS
TGATTCAAACTCCATTCCGA+CGG 0.656357 2:-16480405 None:intergenic
TTGGAGTTAATGATAATGAG+TGG 0.659577 2:+16481456 MsaT007323.1:CDS
AGTGGAATCTAATAGGTGGA+GGG 0.661358 2:+16487127 MsaT007323.1:CDS
AATGATTAGACTGAGAGTAG+TGG 0.669263 2:+16487109 MsaT007323.1:CDS
GCACTTGTGATAGATACAGA+AGG 0.672329 2:+16480795 MsaT007323.1:CDS
TGGCGAACATGAAAATGGAA+GGG 0.678993 2:+16486806 MsaT007323.1:CDS
CGAACATGAAAATGGAAGGG+AGG 0.681120 2:+16486809 MsaT007323.1:CDS
GCGTTTGATCGAGAAACAAG+CGG 0.681684 2:+16481868 MsaT007323.1:CDS
CTACTTGAGGTTGAGGACAA+GGG 0.687690 2:+16486738 MsaT007323.1:CDS
TTAGTTGGAATAAATCACCA+TGG 0.700334 2:+16481104 MsaT007323.1:CDS
TCACATAGGAGGATGGACAG+TGG 0.701404 2:+16487212 MsaT007323.1:CDS
ACTATTTCCAGATAGATGAG+GGG 0.701880 2:+16486697 MsaT007323.1:intron
ACATAGGAGGATGGACAGTG+GGG 0.705660 2:+16487214 MsaT007323.1:CDS
CGTAACAGTAAAGAGAAGCG+TGG 0.709182 2:+16486999 MsaT007323.1:CDS
TCAGAGATGGAGGATATGCG+AGG 0.712448 2:+16480646 MsaT007323.1:CDS
TGCCTAGTTCTGATGCAGAG+GGG 0.720033 2:+16480751 MsaT007323.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 16480266 16489381 16480266 ID=MsaG007323
Chr2 mRNA 16480266 16489381 16480266 ID=MsaT007323.1;Parent=MsaG007323
Chr2 exon 16480266 16480667 16480266 ID=MsaT007323.1.exon1;Parent=MsaT007323.1
Chr2 CDS 16480266 16480667 16480266 ID=cds.MsaT007323.1;Parent=MsaT007323.1
Chr2 exon 16480702 16482263 16480702 ID=MsaT007323.1.exon2;Parent=MsaT007323.1
Chr2 CDS 16480702 16482263 16480702 ID=cds.MsaT007323.1;Parent=MsaT007323.1
Chr2 exon 16482448 16482466 16482448 ID=MsaT007323.1.exon3;Parent=MsaT007323.1
Chr2 CDS 16482448 16482466 16482448 ID=cds.MsaT007323.1;Parent=MsaT007323.1
Chr2 exon 16486708 16487395 16486708 ID=MsaT007323.1.exon4;Parent=MsaT007323.1
Chr2 CDS 16486708 16487395 16486708 ID=cds.MsaT007323.1;Parent=MsaT007323.1
Chr2 exon 16488149 16488438 16488149 ID=MsaT007323.1.exon5;Parent=MsaT007323.1
Chr2 CDS 16488149 16488438 16488149 ID=cds.MsaT007323.1;Parent=MsaT007323.1
Chr2 exon 16489271 16489381 16489271 ID=MsaT007323.1.exon6;Parent=MsaT007323.1
Chr2 CDS 16489271 16489381 16489271 ID=cds.MsaT007323.1;Parent=MsaT007323.1
Gene Sequence

>MsaT007323.1

ATGGAAGATACTTCACCATGCAGCAAGCAATCGCCGGATGGAGAATGCATTGAAATTCCAAGAGATGAAGATGGTGCTCTAATTGAATTTGATTGTCAAGACGATATTTCTGAGGGTAAAAAGGAATTTGTTGCACCTGCCGTCGGAATGGAGTTTGAATCATATGATGATGCTTACAATTATTATATTTGCTATGCCAAAGAGGTTGGATTTTGTGTGAGGATTAAGAATTCATGGTTCAAGCGAAATAGCAAAGAAAAATACGGTGCAGTGCTTTGTTGTAGCAGTCAAGGTTTCAAAAGAATAAAAGATGTTAACAATTTGAGAAAAGAAACCAGAACTGGTTGTCCTGCCATGATAAGGATTAAGTTAGTGGAGTCTCAGAGATGGAGGATATGCGAGATACATAAATCCATTAAAAAGATGGGAAGTGGCACTAAAAATAACTCGTTGCCTAGTTCTGATGCAGAGGGGAAAACAATTAAGGTATATCATGCACTTGTGATAGATACAGAAGGTAATGACAATTTAAACTCCAATGCAAGAGATGACAGAGCTTTCTCCAAATATTCTAATAAGTTGAACCTAAGAAAAGGTGACACACAAGCCATTTATAATTTCCTCTGCCGTATGCAATTGACTAATCCAAACTTCTTTTATTTGATGGATTTCAATGATGAAGGGCATTTAAGGAATGCATTATGGGTTGATGCTAAATCTAGGGCTGCATGCAGTTATTTTAGTGATGTCATTTATCTTGACAACACTTATTTGGTAAACAAATATGAAATCCCTCTTGTGGCTTTAGTTGGAATAAATCACCATGGTCAATCAGTGTTATTAGGTTGTGGCCTGCTTGCAGGTGAAATAATAGAATCATATAAATGGTTGTTTAGAACATGGAACACATGTATATCAGGTTGTTCTCCTCAAACTATTATTACAGATAGATGCAAAGCTTTGCAGAGTGTAATTGCTGAAGTTTTTCCCAGATCTCATCATTGCTTTAGCTTGTCATTAATTATGAAAAAAGTACCAGAAAAACTGGGAGGGTTGCACAATTATGATGCAATCAAGAAAGCGCTGATTAAAGCAGTTTATGAGACATCAAAAGTGATTGAATTCGATGCAGCATGGGGATTCATGATTCAATGTTTTGGAGTTAATGATAATGAGTGGCTTTGTTCACTTTACGAAGACCGAGTTCATTGGGCACCGGTATATCTGAAGGACAAGTTTTTTGCTGGTATGTCTGCAACACGGTCTGGGGAAAGCATTAGTCCTTTTTTCGATAAATATGTCCACAAACAGACTTCTTTGAAGGAGTTTCTTGACAAGTATGAATTAGCTCTTTACAAGAAGTACAAGGAAGAATCTCTTGCAGATATTGAATCGAGAAGCTCAAGCCCTTTGCTAAAAACCAAATGCTCTTTTGAACTGCAGCTCTCTAGTATGTACACAAGAGAATTATTCACCAAGTTCCAACTTGAAGTGGAAGAAATGTTCTCTTGTTTTGGGACAATGCAATTACACGTGGATGGCCCTATCACCGTCTTCTTGGTCAAGGAGCGTTTGATCGAGAAACAAGCGGAAATTAAGGATTTTGAAGTTGTGTATAGTAAAACATCAGTTGAAGTTCGTTGTATCTGTTGTTGCTTTAATTTTTATGGATATCTTTGTCGGCATGCGTTGTGTGTACTCAATTTTATTGGGGTGGAGGAGATTCCTCCTAAGTACATTCTTTCGCGGTGGAATAAAGATTACAAGCGCTTTTGTATTCCCGGTCACAACCGTTTTAGTCCTGATGATACTGATACTGACCCTATTCAATGGTCTAATCGGTTATTTAGAAGTGCCTTACAAGTTGTGGAGGAGGGAGTAATTTCTCTGGATCATTACAAGGTAGCGTTGCAGGCTTTTGAAGAATCACTTCATCAGGTTCGTGATGTAGAACACAAACAGGATGGCGGTAACACTTTGGAAATAGATGAGGGGTCTCTACAAAGTAAGCAACTACTTGAGGTTGAGGACAAGGGCAACGAGCTTGAGAATGACTGTAGTCAATTGTTTGAGATCGATGGTGGCGAACATGAAAATGGAAGGGAGGAAACCACGGGAGTTGACAGTCATAGTGAAGAATCTCAAAGAACAAAAGACTACGCCCAACCTGTTGAGGGAGTGGAGTTTGAAACTTATGATGATGCCTACAACTATTATAATAGCTATGCCAGGGAAATTGGATTTGCTATTCGGGTTAAATCTTCGTGGACCAAACGTAACAGTAAAGAGAAGCGTGGTGCTGTCTTGTGTTGTAGTTGTGAAGGTTTCAAAACAAATAAAGAAGCTAATAGTCGTAGGAAAGAAACAAGAACAGGCTGTCTAGCAATGATTAGACTGAGAGTAGTGGAATCTAATAGGTGGAGGGTGGATGAAGTCAAGCTTGAGCATAACCATTCATTTGATCCTGAAAGAGCGCAAAACTCTAAATCACATAGGAGGATGGACAGTGGGGCCAAAAGAAAGGTTGAGCCGACTCTAGATGTTGAAGTACGGACAATCAAGTTATATCGAATGCCAACTGTAGATGCTTCCGGTTATGGGAGCTCAAATTCTAATGAAGGAGGAACTAGCAACAACGTTAAGTTTTCCAGGAGACTGAAGCTAAAAAAAGTTCAACAAAAAAAGCAAAAAACTGAAGCTCTTGATGATTTGGAGTCACAAAATTCAAGTCCACAGCTGAAAACAAGATGCCACTATGAGTTGCAACTCTCTAAATTGTACACGAATGCAATATTCAATAAGTTCCAAGATGAGGTGGTGATGATGTCTTCCTGTTTCAGCGTCTCACAAATTCAAACAAACGAGTCTATAGTGACATACATGGTAAAAGAACATCAAGGGGAAGAACCAGTAAGAAATGATAGGCATTTTGAAGTCATGTATGATAAAGCATTAACCGAACTCGGAAGAAGGATAACTCGCTGCAAGTGTCACATCCATGATTACTACTATGGTGTTGCATTGCCATTAATAAGTGCTTTCGTGCAAAATAGTAATGATGTGTCAAACTAG
Protein sequence

>MsaT007323.1

MEDTSPCSKQSPDGECIEIPRDEDGALIEFDCQDDISEGKKEFVAPAVGMEFESYDDAYNYYICYAKEVGFCVRIKNSWFKRNSKEKYGAVLCCSSQGFKRIKDVNNLRKETRTGCPAMIRIKLVESQRWRICEIHKSIKKMGSGTKNNSLPSSDAEGKTIKVYHALVIDTEGNDNLNSNARDDRAFSKYSNKLNLRKGDTQAIYNFLCRMQLTNPNFFYLMDFNDEGHLRNALWVDAKSRAACSYFSDVIYLDNTYLVNKYEIPLVALVGINHHGQSVLLGCGLLAGEIIESYKWLFRTWNTCISGCSPQTIITDRCKALQSVIAEVFPRSHHCFSLSLIMKKVPEKLGGLHNYDAIKKALIKAVYETSKVIEFDAAWGFMIQCFGVNDNEWLCSLYEDRVHWAPVYLKDKFFAGMSATRSGESISPFFDKYVHKQTSLKEFLDKYELALYKKYKEESLADIESRSSSPLLKTKCSFELQLSSMYTRELFTKFQLEVEEMFSCFGTMQLHVDGPITVFLVKERLIEKQAEIKDFEVVYSKTSVEVRCICCCFNFYGYLCRHALCVLNFIGVEEIPPKYILSRWNKDYKRFCIPGHNRFSPDDTDTDPIQWSNRLFRSALQVVEEGVISLDHYKVALQAFEESLHQVRDVEHKQDGGNTLEIDEGSLQSKQLLEVEDKGNELENDCSQLFEIDGGEHENGREETTGVDSHSEESQRTKDYAQPVEGVEFETYDDAYNYYNSYAREIGFAIRVKSSWTKRNSKEKRGAVLCCSCEGFKTNKEANSRRKETRTGCLAMIRLRVVESNRWRVDEVKLEHNHSFDPERAQNSKSHRRMDSGAKRKVEPTLDVEVRTIKLYRMPTVDASGYGSSNSNEGGTSNNVKFSRRLKLKKVQQKKQKTEALDDLESQNSSPQLKTRCHYELQLSKLYTNAIFNKFQDEVVMMSSCFSVSQIQTNESIVTYMVKEHQGEEPVRNDRHFEVMYDKALTELGRRITRCKCHIHDYYYGVALPLISAFVQNSNDVSN*