AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007400


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007400 MtrunA17_Chr2g0292411 98.551 207 3 0 1 207 1 207 2.57e-153 423
MsaG007400 MtrunA17_Chr3g0103391 51.429 140 52 4 48 184 116 242 8.76e-37 130
MsaG007400 MtrunA17_Chr8g0352251 62.162 111 38 1 86 192 127 237 2.54e-36 128
MsaG007400 MtrunA17_Chr8g0343451 52.381 126 49 3 49 173 110 225 2.09e-35 127
MsaG007400 MtrunA17_Chr2g0329811 43.750 160 82 5 47 199 118 276 6.59e-34 123
MsaG007400 MtrunA17_Chr2g0300261 44.444 144 71 3 62 204 32 167 4.48e-32 116
MsaG007400 MtrunA17_Chr7g0267581 42.138 159 69 5 49 204 122 260 7.71e-30 112
MsaG007400 MtrunA17_Chr1g0177091 43.537 147 59 6 44 186 111 237 2.34e-29 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007400 AT1G30500.2 54.146 205 73 7 1 203 1 186 3.93e-65 199
MsaG007400 AT1G30500.1 53.171 205 71 7 1 203 1 182 6.31e-63 194
MsaG007400 AT2G34720.1 50.000 212 83 8 1 206 1 195 3.81e-58 182
MsaG007400 AT2G34720.2 59.060 149 51 4 62 206 18 160 7.41e-54 170
MsaG007400 AT2G34720.3 59.060 149 51 4 62 206 18 160 7.41e-54 170
MsaG007400 AT3G20910.1 45.625 160 73 3 48 204 112 260 2.13e-37 132
MsaG007400 AT5G12840.5 46.108 167 72 4 47 207 118 272 7.27e-37 130
MsaG007400 AT5G12840.3 46.108 167 72 4 47 207 118 272 7.27e-37 130
MsaG007400 AT5G12840.1 46.108 167 72 4 47 207 118 272 7.27e-37 130
MsaG007400 AT5G12840.4 45.509 167 73 4 47 207 117 271 7.99e-37 129
MsaG007400 AT5G12840.2 45.509 167 73 4 47 207 117 271 7.99e-37 129
MsaG007400 AT1G72830.1 47.552 143 56 4 61 192 134 268 3.54e-34 124
MsaG007400 AT1G54160.1 57.843 102 40 2 86 184 148 249 8.19e-33 120
MsaG007400 AT1G72830.2 47.222 144 56 5 61 192 134 269 2.17e-32 119
MsaG007400 AT1G17590.3 50.000 120 46 3 61 179 132 238 9.76e-31 115
MsaG007400 AT1G17590.2 50.000 120 46 3 61 179 132 238 9.76e-31 115
MsaG007400 AT1G17590.4 50.000 120 46 3 61 179 132 238 9.76e-31 115
MsaG007400 AT1G17590.1 50.000 120 46 3 61 179 132 238 9.76e-31 115
MsaG007400 AT3G14020.2 54.808 104 42 2 73 175 134 233 3.43e-29 110
MsaG007400 AT3G14020.1 54.808 104 42 2 73 175 134 233 3.43e-29 110
MsaG007400 AT5G06510.4 40.559 143 61 5 76 203 2 135 9.81e-25 95.9
MsaG007400 AT5G06510.7 40.559 143 61 5 76 203 2 135 9.81e-25 95.9
MsaG007400 AT5G06510.1 46.364 110 46 3 104 203 124 230 2.61e-24 97.1
MsaG007400 AT5G06510.5 46.364 110 46 3 104 203 124 230 2.61e-24 97.1
MsaG007400 AT5G06510.2 46.364 110 46 3 104 203 124 230 2.61e-24 97.1
MsaG007400 AT5G06510.6 46.364 110 46 3 104 203 124 230 2.61e-24 97.1
MsaG007400 AT5G06510.3 46.364 110 46 3 104 203 75 181 2.72e-24 95.9
MsaG007400 AT3G05690.1 42.609 115 42 2 62 172 105 199 3.57e-23 94.7
MsaG007400 AT3G05690.2 42.609 115 42 2 62 172 142 236 8.12e-23 94.4
MsaG007400 AT1G72830.3 54.878 82 27 1 121 192 162 243 3.09e-22 92.4

Find 55 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAGAATTGAGATTGATAT+TGG 0.249770 2:+17628447 None:intergenic
CCAGGCCAATCTTCAGTTAA+TGG 0.300525 2:-17629371 MsaT007400.1:intron
CGGATAAGGTTGTGCATCAT+AGG 0.313332 2:+17629516 None:intergenic
CTGTAGTAGGGATCCGGATA+TGG 0.349828 2:+17629553 None:intergenic
CCGTAGGGTTGCGGCGGATA+AGG 0.368018 2:+17629502 None:intergenic
GAAATTCCGTTTGCAGATAT+AGG 0.399724 2:+17631343 None:intergenic
AATTCCCATTAACTGAAGAT+TGG 0.417171 2:+17629366 None:intergenic
CTTTCTTTGAATTCAGAAAC+CGG 0.421740 2:+17628515 None:intergenic
AGCAAAGATGCTTCTGTAGT+AGG 0.425029 2:+17629540 None:intergenic
CAGGCCAATCTTCAGTTAAT+GGG 0.435926 2:-17629370 MsaT007400.1:CDS
TGTCGTGATTCATGCAAGTA+TGG 0.440305 2:+17628574 None:intergenic
AAAAGAAAGTTACACGGAAT+CGG 0.459956 2:-17629228 MsaT007400.1:CDS
GCACCTCCACAGCTTGGCAC+GGG 0.466695 2:-17631280 MsaT007400.1:CDS
ACCACCTGCTTATCCATATC+CGG 0.479165 2:-17629566 MsaT007400.1:CDS
CTGAAAAGAGCAAGAGGATG+TGG 0.485801 2:-17628541 MsaT007400.1:CDS
CGCCGGTATTGATAATGCTC+AGG 0.487682 2:-17631317 MsaT007400.1:CDS
GCAAAGATGCTTCTGTAGTA+GGG 0.499527 2:+17629541 None:intergenic
GTCAATGCAAAACAGTATCA+TGG 0.506073 2:-17629292 MsaT007400.1:CDS
ATGCTTCTGTAGTAGGGATC+CGG 0.507422 2:+17629547 None:intergenic
GGATAAGGTTGTGCATCATA+GGG 0.522061 2:+17629517 None:intergenic
GCTTGGCACGGGGCATGCTA+TGG 0.522353 2:-17631269 MsaT007400.1:intron
GGATGTCCACCGTAGGGTTG+CGG 0.523458 2:+17629493 None:intergenic
AGCACCTCCACAGCTTGGCA+CGG 0.523882 2:-17631281 MsaT007400.1:CDS
TTTGCCAACTGATGCAGTTG+AGG 0.525775 2:-17629326 MsaT007400.1:CDS
GCATGCCCCGTGCCAAGCTG+TGG 0.532400 2:+17631274 None:intergenic
GAGCAAGAGGATGTGGAGGC+CGG 0.538187 2:-17628534 MsaT007400.1:CDS
GAGAATCAACAAGATGAGGT+TGG 0.539095 2:-17628490 MsaT007400.1:CDS
CCTTATCCGCCGCAACCCTA+CGG 0.540441 2:-17629502 MsaT007400.1:CDS
TGCCCCGTGCCAAGCTGTGG+AGG 0.541599 2:+17631277 None:intergenic
GGATCCGGATATGGATAAGC+AGG 0.543946 2:+17629562 None:intergenic
GGCTCCTCAACTGCATCAGT+TGG 0.545637 2:+17629322 None:intergenic
AACCTGAGCATTATCAATAC+CGG 0.557780 2:+17631315 None:intergenic
ACCCTACGGTGGACATCCAA+TGG 0.560327 2:-17629488 MsaT007400.1:intron
ATGCAGCCTATATCTGCAAA+CGG 0.566429 2:-17631349 MsaT007400.1:CDS
CACCTCCACAGCTTGGCACG+GGG 0.573071 2:-17631279 MsaT007400.1:CDS
TGTCCAGATAACGAGGCTGA+TGG 0.582397 2:-17631394 MsaT007400.1:intron
ACCATTGGATGTCCACCGTA+GGG 0.583635 2:+17629487 None:intergenic
CACGCTCTGAAAAGAGCAAG+AGG 0.590582 2:-17628547 MsaT007400.1:CDS
TATGCAGCACCTCCACAGCT+TGG 0.593812 2:-17631286 MsaT007400.1:CDS
AGATGAGAATCAACAAGATG+AGG 0.599218 2:-17628494 MsaT007400.1:CDS
TCAACTGCATCAGTTGGCAA+AGG 0.603408 2:+17629328 None:intergenic
GGATATGGATAAGCAGGTGG+TGG 0.616937 2:+17629568 None:intergenic
GAAAGTTACACGGAATCGGA+AGG 0.621098 2:-17629224 MsaT007400.1:intron
TATCCGCCGCAACCCTACGG+TGG 0.624811 2:-17629499 MsaT007400.1:CDS
TTAATGGGAATTCAGCATGC+AGG 0.625390 2:-17629355 MsaT007400.1:CDS
ATCATGTGTGGAAGAAGTCA+TGG 0.636060 2:+17632445 None:intergenic
TCTTCCACACATGATCCCAC+AGG 0.647344 2:-17632437 MsaT007400.1:intron
TGTCCACCGTAGGGTTGCGG+CGG 0.648752 2:+17629496 None:intergenic
AACCATTGGATGTCCACCGT+AGG 0.650023 2:+17629486 None:intergenic
AAAAGAGCAAGAGGATGTGG+AGG 0.656650 2:-17628538 MsaT007400.1:CDS
AATACCTGTGGGATCATGTG+TGG 0.669627 2:+17632433 None:intergenic
TCCGGATATGGATAAGCAGG+TGG 0.669883 2:+17629565 None:intergenic
AAATTGTTGTCCAGATAACG+AGG 0.691918 2:-17631401 MsaT007400.1:intron
AATCAGAAAAGAAAGTTACA+CGG 0.697568 2:-17629234 MsaT007400.1:CDS
GCAAACGGAATTTCTCACGC+CGG 0.724226 2:-17631334 MsaT007400.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 17628411 17632465 17628411 ID=MsaG007400
Chr2 mRNA 17628411 17632465 17628411 ID=MsaT007400.1;Parent=MsaG007400
Chr2 exon 17628411 17628596 17628411 ID=MsaT007400.1.exon5;Parent=MsaT007400.1
Chr2 CDS 17628411 17628596 17628411 ID=cds.MsaT007400.1;Parent=MsaT007400.1
Chr2 exon 17629225 17629389 17629225 ID=MsaT007400.1.exon4;Parent=MsaT007400.1
Chr2 CDS 17629225 17629389 17629225 ID=cds.MsaT007400.1;Parent=MsaT007400.1
Chr2 exon 17629489 17629593 17629489 ID=MsaT007400.1.exon3;Parent=MsaT007400.1
Chr2 CDS 17629489 17629593 17629489 ID=cds.MsaT007400.1;Parent=MsaT007400.1
Chr2 exon 17631270 17631409 17631270 ID=MsaT007400.1.exon2;Parent=MsaT007400.1
Chr2 CDS 17631270 17631409 17631270 ID=cds.MsaT007400.1;Parent=MsaT007400.1
Chr2 exon 17632438 17632465 17632438 ID=MsaT007400.1.exon1;Parent=MsaT007400.1
Chr2 CDS 17632438 17632465 17632438 ID=cds.MsaT007400.1;Parent=MsaT007400.1
Gene Sequence

>MsaT007400.1

ATGACTTCTTCCACACATGATCCCACAGATAACGAGGCTGATGGACAGCAGCAGTCAGAATCACAGATGCAGCCTATATCTGCAAACGGAATTTCTCACGCCGGTATTGATAATGCTCAGGTTGTTCAGTATGCAGCACCTCCACAGCTTGGCACGGGGCATGCTATGGCACCACCACCTGCTTATCCATATCCGGATCCCTACTACAGAAGCATCTTTGCTCCCTATGATGCACAACCTTATCCGCCGCAACCCTACGGTGGACATCCAATGGCCAATCTTCAGTTAATGGGAATTCAGCATGCAGGAGTTCCTTTGCCAACTGATGCAGTTGAGGAGCCTGTGTTTGTCAATGCAAAACAGTATCATGGTATTTTAAGACGCAGGCAGTCTCGTGCTAAAGCTGAATCAGAAAAGAAAGTTACACGGAATCGGAAGCCATACTTGCATGAATCACGACATTTGCACGCTCTGAAAAGAGCAAGAGGATGTGGAGGCCGGTTTCTGAATTCAAAGAAAGATGAGAATCAACAAGATGAGGTTGGATCAGCTGACAATTCACATTCCAATATCAATCTCAATTCTGATAGAAATGATCTTGCACAATCTGATAAAAATCCTTGA

Protein sequence

>MsaT007400.1

MTSSTHDPTDNEADGQQQSESQMQPISANGISHAGIDNAQVVQYAAPPQLGTGHAMAPPPAYPYPDPYYRSIFAPYDAQPYPPQPYGGHPMANLQLMGIQHAGVPLPTDAVEEPVFVNAKQYHGILRRRQSRAKAESEKKVTRNRKPYLHESRHLHALKRARGCGGRFLNSKKDENQQDEVGSADNSHSNINLNSDRNDLAQSDKNP*