AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007431

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007431 MtrunA17_Chr2g0292731 88.810 563 52 1 148 710 1 552 0.0 1040
MsaG007431 MtrunA17_Chr2g0292741 86.667 555 58 2 156 710 1 539 0.0 996
MsaG007431 MtrunA17_Chr6g0481041 40.405 740 390 16 1 706 1 723 7.54e-173 512
MsaG007431 MtrunA17_Chr4g0024351 36.034 716 409 17 7 706 47 729 9.32e-144 437
MsaG007431 MtrunA17_Chr2g0322731 37.094 709 411 15 9 706 81 765 1.03e-143 438
MsaG007431 MtrunA17_Chr8g0350321 35.901 727 416 20 9 706 101 806 2.75e-136 421
MsaG007431 MtrunA17_Chr7g0246631 35.773 724 413 17 9 706 77 774 5.65e-136 419
MsaG007431 MtrunA17_Chr1g0203671 34.406 715 415 18 25 706 96 789 3.65e-126 394
MsaG007431 MtrunA17_Chr5g0410711 33.468 744 437 22 1 709 61 781 1.75e-124 389
MsaG007431 MtrunA17_Chr2g0331581 32.701 685 417 13 28 706 143 789 4.34e-117 370
MsaG007431 MtrunA17_Chr6g0472471 33.012 727 405 22 28 706 73 765 5.32e-117 369
MsaG007431 MtrunA17_Chr2g0292761 37.175 616 301 17 103 707 1 541 4.02e-115 357
MsaG007431 MtrunA17_Chr5g0394111 32.659 692 365 17 32 707 41 647 1.42e-103 330
MsaG007431 MtrunA17_Chr7g0224451 36.917 493 237 12 25 509 17 443 8.74e-92 293
MsaG007431 MtrunA17_Chr2g0304191 88.971 136 15 0 230 365 1 136 5.58e-81 253
MsaG007431 MtrunA17_Chr7g0224481 36.939 379 175 9 25 395 17 339 7.43e-65 218
MsaG007431 MtrunA17_Chr7g0224491 37.188 320 163 7 398 706 1 293 8.76e-55 189
MsaG007431 MtrunA17_Chr8g0349941 95.556 45 2 0 465 509 67 111 2.80e-23 95.1
MsaG007431 MtrunA17_Chr2g0319671 32.018 228 131 7 226 449 63 270 5.13e-23 100
MsaG007431 MtrunA17_Chr6g0460611 45.055 91 50 0 85 175 2 92 1.99e-22 92.0
MsaG007431 MtrunA17_Chr5g0419821 47.000 100 52 1 23 121 32 131 5.25e-19 85.1
MsaG007431 MtrunA17_Chr2g0319681 26.638 229 160 4 482 706 1 225 2.40e-17 82.4
MsaG007431 MtrunA17_Chr7g0224431 32.468 154 62 3 249 401 4 116 1.07e-12 65.5
MsaG007431 MtrunA17_Chr2g0319691 31.579 114 75 2 336 449 1 111 4.12e-12 64.7
MsaG007431 MtrunA17_Chr2g0319701 25.946 185 117 5 533 706 48 223 1.29e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007431 AT1G73360.1 38.928 709 384 14 22 706 30 713 1.97e-160 480
MsaG007431 AT1G17920.2 38.081 688 386 15 28 706 25 681 1.74e-147 446
MsaG007431 AT1G17920.1 38.081 688 386 15 28 706 25 681 1.74e-147 446
MsaG007431 AT3G03260.1 38.483 725 386 17 7 706 5 694 4.04e-147 445
MsaG007431 AT1G05230.3 36.364 704 403 13 8 706 51 714 7.86e-146 442
MsaG007431 AT1G05230.8 36.261 706 403 14 8 706 51 716 1.01e-143 437
MsaG007431 AT1G05230.4 36.261 706 403 14 8 706 51 716 1.01e-143 437
MsaG007431 AT1G05230.9 36.261 706 403 14 8 706 51 716 1.01e-143 437
MsaG007431 AT1G05230.10 36.261 706 403 14 8 706 51 716 1.01e-143 437
MsaG007431 AT1G05230.7 36.261 706 403 14 8 706 51 716 1.01e-143 437
MsaG007431 AT1G05230.11 36.261 706 403 14 8 706 51 716 1.01e-143 437
MsaG007431 AT1G05230.2 36.261 706 403 14 8 706 51 716 1.01e-143 437
MsaG007431 AT1G05230.6 36.261 706 403 14 8 706 51 716 1.01e-143 437
MsaG007431 AT1G05230.5 36.261 706 403 14 8 706 51 716 1.01e-143 437
MsaG007431 AT1G05230.1 36.261 706 403 14 8 706 51 716 1.01e-143 437
MsaG007431 AT4G04890.2 36.401 728 414 17 2 706 34 735 4.31e-141 431
MsaG007431 AT4G04890.1 36.401 728 414 17 2 706 34 735 4.31e-141 431
MsaG007431 AT4G21750.2 36.657 712 404 15 25 706 63 757 1.27e-138 425
MsaG007431 AT4G21750.1 36.657 712 404 15 25 706 63 757 1.27e-138 425
MsaG007431 AT4G21750.3 36.657 712 404 15 25 706 63 757 1.27e-138 425
MsaG007431 AT4G21750.4 36.657 712 404 15 25 706 63 757 1.27e-138 425
MsaG007431 AT5G17320.1 35.180 722 411 18 17 706 19 715 5.37e-127 394
MsaG007431 AT4G00730.1 34.566 703 414 18 20 706 126 798 5.56e-125 391
MsaG007431 AT3G61150.1 33.379 731 428 21 11 706 97 803 9.55e-123 385
MsaG007431 AT1G05230.12 36.021 583 337 10 8 586 51 601 1.59e-120 374
MsaG007431 AT1G34650.1 35.584 711 393 20 32 706 24 705 4.67e-119 373
MsaG007431 AT3G03260.2 39.893 559 302 12 7 556 5 538 1.85e-117 363
MsaG007431 AT5G52170.3 34.366 710 379 20 11 706 42 678 9.65e-116 363
MsaG007431 AT5G52170.1 34.366 710 379 20 11 706 42 678 9.65e-116 363
MsaG007431 AT5G52170.2 34.406 715 377 21 11 706 42 683 4.83e-115 362
MsaG007431 AT5G46880.3 31.474 753 431 21 8 710 93 810 1.04e-109 352
MsaG007431 AT5G46880.1 31.474 753 431 21 8 710 93 810 1.04e-109 352
MsaG007431 AT5G46880.2 31.474 753 431 21 8 710 93 810 1.04e-109 352
MsaG007431 AT1G79840.3 32.853 694 401 19 25 706 44 684 5.13e-105 335
MsaG007431 AT1G79840.6 32.853 694 401 19 25 706 44 684 5.13e-105 335
MsaG007431 AT2G32370.1 31.918 730 416 22 8 704 37 718 1.34e-104 335
MsaG007431 AT1G79840.1 32.853 694 401 19 25 706 102 742 1.96e-104 336
MsaG007431 AT1G79840.2 32.853 694 401 19 25 706 131 771 5.31e-104 335
MsaG007431 AT4G17710.1 30.213 705 391 18 23 710 87 707 1.04e-95 311
MsaG007431 AT4G25530.1 29.196 709 449 16 8 706 17 682 8.67e-88 290
MsaG007431 AT1G79840.4 32.746 568 325 14 25 584 102 620 1.37e-87 288
MsaG007431 AT4G00730.2 35.535 439 250 10 20 447 126 542 2.75e-80 267
MsaG007431 AT1G79840.5 32.366 448 248 12 25 464 102 502 2.21e-60 212
MsaG007431 AT5G07260.2 20.253 632 394 15 83 706 3 532 1.02e-24 109
MsaG007431 AT5G07260.1 20.283 636 394 15 83 706 3 537 1.09e-24 109

Find 164 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCACCAATCCTGAATATT+TGG 0.136860 2:-17946208 None:intergenic
AAACTATGGATGTGGCTTTA+AGG 0.147288 2:+17948397 MsaT007431.1:CDS
CCAAAGAACAATCACTTTAA+AGG 0.153190 2:+17946162 MsaT007431.1:CDS
ATGGACTGGTGCTCAGAATT+TGG 0.173529 2:-17948551 None:intergenic
ATCGCTTTGTAGGATCTTTA+AGG 0.190512 2:-17947719 None:intergenic
TAGTGGGATGGTCTTTCTTA+TGG 0.230215 2:+17948066 MsaT007431.1:intron
GTGTACCCATCTTGAGTATT+TGG 0.232783 2:-17946108 None:intergenic
AAACCAAAACTTGATTTGTT+TGG 0.252374 2:-17944896 None:intergenic
TTTGGAGATTTGACCCAAAA+AGG 0.264891 2:-17946090 None:intergenic
CCTTTAAAGTGATTGTTCTT+TGG 0.271566 2:-17946162 None:intergenic
AACCAAAACTTGATTTGTTT+GGG 0.288547 2:-17944895 None:intergenic
GAAGAATCTTCCAAATATTC+AGG 0.291428 2:+17946198 MsaT007431.1:CDS
GTTACCTGTGATCGCTTTGT+AGG 0.304101 2:-17947729 None:intergenic
AGCCCAAACAAATCAAGTTT+TGG 0.306856 2:+17944893 MsaT007431.1:CDS
TATTCCTCCCTTCAAGTGTT+TGG 0.321521 2:-17947476 None:intergenic
GTTGGCGACTATAATTTATA+CGG 0.324947 2:+17947236 MsaT007431.1:CDS
AAAGCTCCATCTCGACTTCC+AGG 0.326040 2:-17946569 None:intergenic
TGACGTCGTTCTGCCTCGTC+TGG 0.329592 2:-17944847 None:intergenic
CGGAGCTGAAAGCTGGATTA+AGG 0.349348 2:+17947256 MsaT007431.1:CDS
CCTCCTATGGTTTCTTCAAC+AGG 0.351635 2:-17947329 None:intergenic
TGAAATGCAGTTGCCATGAT+AGG 0.356217 2:-17948127 None:intergenic
CAATGCTATCAAGAGTTAAA+TGG 0.356804 2:-17947575 None:intergenic
AACTATGGATGTGGCTTTAA+GGG 0.361467 2:+17948398 MsaT007431.1:CDS
TCAGCTTTGGTAACAATTGT+AGG 0.366355 2:-17946518 None:intergenic
TGGACTGGTGCTCAGAATTT+GGG 0.370714 2:-17948550 None:intergenic
TTACTGCTGCTACCACCATT+TGG 0.373536 2:+17947664 MsaT007431.1:CDS
CCAAATCTTAATGCACCATT+TGG 0.373891 2:+17948483 MsaT007431.1:CDS
TCGAGATGGAGCTTTATTAC+TGG 0.373995 2:+17946577 MsaT007431.1:CDS
CACTTTGATAGTTTCAGCTT+TGG 0.378308 2:-17946531 None:intergenic
CTCTATACATGTAATGTGTA+TGG 0.392878 2:-17947208 None:intergenic
ATTAGTAACTTTCGAGATGA+TGG 0.395166 2:+17943642 MsaT007431.1:CDS
ACTATCAAAGTGTTTGAAAT+CGG 0.396566 2:+17946542 MsaT007431.1:CDS
CCAAATGGTGCATTAAGATT+TGG 0.397130 2:-17948483 None:intergenic
AGAATGTGTGAGAGATCTTT+AGG 0.399513 2:+17947287 MsaT007431.1:CDS
GTCAATTGGCTGTGAAAGTT+GGG 0.402996 2:+17944866 MsaT007431.1:CDS
GAGTGCTCAAATCATGTTCA+TGG 0.409107 2:+17945937 MsaT007431.1:CDS
TTAGAATAGTAGCTGCCAAT+TGG 0.411318 2:-17948623 None:intergenic
CACTCATAAACCGTTCGGTT+TGG 0.412582 2:-17945919 None:intergenic
AGCACTTGGTCAACTGCATA+TGG 0.416823 2:-17948570 None:intergenic
CCACAATTTGCGAAGCTTGT+GGG 0.416831 2:+17945590 MsaT007431.1:CDS
CGTCAATTGGCTGTGAAAGT+TGG 0.419536 2:+17944865 MsaT007431.1:CDS
GAAATTGCACACATTTCAAA+TGG 0.421250 2:+17948102 MsaT007431.1:CDS
AGCGGAGGTAAGACGTTTGT+AGG 0.421913 2:-17943676 None:intergenic
GAACAAATAACAAAACCATT+TGG 0.423668 2:-17948450 None:intergenic
CGAGGCAGAACGACGTCAAT+TGG 0.430389 2:+17944852 MsaT007431.1:CDS
CGAGAAAGAGGTGCAGTGTT+TGG 0.431308 2:-17946823 None:intergenic
AAATCTCCAAATACTCAAGA+TGG 0.432047 2:+17946102 MsaT007431.1:CDS
ATGGGAGGTGGCGGTGAGTC+CGG 0.432550 2:+17943609 MsaT007431.1:CDS
GAAGATTAGTGGCATAGATT+TGG 0.434141 2:+17946227 MsaT007431.1:CDS
ACAAAACCATTTGGAAGGAT+TGG 0.438392 2:-17948441 None:intergenic
GTGTTTGAAATCGGTTCGCC+TGG 0.443787 2:+17946551 MsaT007431.1:CDS
TCAGGATTGGTGAAGATTAG+TGG 0.444549 2:+17946216 MsaT007431.1:CDS
TAGAATAGTAGCTGCCAATT+GGG 0.446699 2:-17948622 None:intergenic
ATTTATACGGAGCTGAAAGC+TGG 0.450930 2:+17947249 MsaT007431.1:CDS
AGATGCTGCAATGGATGAAT+TGG 0.453548 2:+17946044 MsaT007431.1:CDS
TCTCCTGCACGAAATGTTGA+TGG 0.453739 2:-17945636 None:intergenic
TACTTATATCAACACCCAAT+TGG 0.455805 2:+17948608 MsaT007431.1:CDS
GCAGTAACAACAGTGCCATT+TGG 0.456929 2:-17947648 None:intergenic
CACAATTTGCGAAGCTTGTG+GGG 0.460810 2:+17945591 MsaT007431.1:CDS
TTGAGAAATGGATTACTCAA+TGG 0.460842 2:+17943590 None:intergenic
AAAGTGTGATTAACCTCCTA+TGG 0.462791 2:-17947342 None:intergenic
CAACTGCATTTCAATTATCA+AGG 0.467731 2:+17948137 MsaT007431.1:CDS
CATCTTCCACATTAAGCACT+TGG 0.467910 2:-17948584 None:intergenic
TCATAAACCGTTCGGTTTGG+TGG 0.470954 2:-17945916 None:intergenic
GAACAAGAGAACTCTGTTGA+AGG 0.473464 2:+17944921 MsaT007431.1:CDS
CATTTAACTCTTGATAGCAT+TGG 0.474351 2:+17947576 MsaT007431.1:CDS
GAAAAGTTGATGCTGGTGTA+TGG 0.477684 2:+17946767 MsaT007431.1:CDS
CTGCACCTCTTTCTCGTGGT+TGG 0.478289 2:+17946830 MsaT007431.1:CDS
TAACAACAGTGCCATTTGGC+TGG 0.481596 2:-17947644 None:intergenic
TCAGTGATCCAAACACTTGA+AGG 0.482426 2:+17947468 MsaT007431.1:intron
TTAAGAGCTTGCTCAAACTC+CGG 0.486029 2:-17945853 None:intergenic
TTGAGCACTCATAAACCGTT+CGG 0.491484 2:-17945924 None:intergenic
TGTGAATGCTTAACAATGCC+TGG 0.491755 2:+17947537 MsaT007431.1:CDS
TGATGGTCTTCCACAGGAAA+TGG 0.495341 2:-17945619 None:intergenic
CCCACAAGCTTCGCAAATTG+TGG 0.495588 2:-17945590 None:intergenic
ATGTGTGCAATTTCATGCAT+TGG 0.495739 2:-17948093 None:intergenic
TGAGAAATGGATTACTCAAT+GGG 0.495986 2:+17943591 None:intergenic
TCATTCGTGAAATGCCTCAT+GGG 0.496695 2:+17946872 MsaT007431.1:CDS
GAGTTACCAATCCTTCCAAA+TGG 0.500630 2:+17948435 MsaT007431.1:CDS
TACATGTATAGAGATCTTGT+TGG 0.502405 2:+17947218 MsaT007431.1:CDS
CAGCTTTGGTAACAATTGTA+GGG 0.502451 2:-17946517 None:intergenic
GGTTGGCATGTTTCTCGACT+CGG 0.505106 2:+17946248 MsaT007431.1:CDS
TATATAATGTATGATCCTAC+AGG 0.508050 2:-17948357 None:intergenic
CGTGGTTGGAAACATCCCTC+TGG 0.513481 2:+17946844 MsaT007431.1:CDS
GTCGTTCTGCCTCGTCTGGA+TGG 0.514221 2:-17944843 None:intergenic
CGAAAATCTCAAAATAAAAG+AGG 0.514839 2:+17945558 MsaT007431.1:CDS
ATTTCAAATGGACCCTATCA+TGG 0.514869 2:+17948114 MsaT007431.1:CDS
TTAACTCTTGATAGCATTGG+TGG 0.517292 2:+17947579 MsaT007431.1:CDS
CTGCAATGGATGAATTGGTT+AGG 0.518596 2:+17946049 MsaT007431.1:CDS
CTTAACAATGCCTGGTCAAG+TGG 0.522028 2:+17947545 MsaT007431.1:CDS
ATCTTCCAAATATTCAGGAT+TGG 0.522094 2:+17946203 MsaT007431.1:CDS
AGGTGCAGTGTTTGGTCGAG+AGG 0.523583 2:-17946815 None:intergenic
CATCCCTACTCAGAGGCAAA+TGG 0.525201 2:+17948308 MsaT007431.1:CDS
TCACCATTTGCCTCTGAGTA+GGG 0.526700 2:-17948311 None:intergenic
AACTCTTGATAGCATTGGTG+GGG 0.529682 2:+17947581 MsaT007431.1:CDS
ACTATGAGGAGAGGTGTGCT+AGG 0.529898 2:+17945967 MsaT007431.1:CDS
GCCACAATTTGCGAAGCTTG+TGG 0.529984 2:+17945589 MsaT007431.1:CDS
GAGGTAAGACGTTTGTAGGA+AGG 0.532797 2:-17943672 None:intergenic
ATTAGTGGCATAGATTTGGT+TGG 0.532831 2:+17946231 MsaT007431.1:CDS
AAATAACAAAACCATTTGGA+AGG 0.532923 2:-17948446 None:intergenic
AAAACTTTCTGAGCAGGCAG+AGG 0.539007 2:-17947690 None:intergenic
TAACTCTTGATAGCATTGGT+GGG 0.540658 2:+17947580 MsaT007431.1:CDS
GAAATGCAGTTGCCATGATA+GGG 0.544552 2:-17948126 None:intergenic
ATCATTCGTGAAATGCCTCA+TGG 0.548977 2:+17946871 MsaT007431.1:CDS
TTTCTGATGAAGTTTCATGA+AGG 0.549807 2:+17944813 MsaT007431.1:intron
GGTCACTTGGGTTGAACATG+TGG 0.552782 2:+17947169 MsaT007431.1:intron
ATACTGTCAGATGCTCCAAA+TGG 0.553262 2:-17948498 None:intergenic
GAAAGTTTCACTTCTCCTGT+AGG 0.556978 2:+17948342 MsaT007431.1:CDS
TACTGCAGAAAAGTTGATGC+TGG 0.557695 2:+17946760 MsaT007431.1:CDS
GATAAAGCTTGCTGATAGAA+TGG 0.559844 2:+17947503 MsaT007431.1:CDS
GTTACTAATGTCTTCATCAC+CGG 0.562506 2:-17943628 None:intergenic
TTCAAGTATGGATGTTTGAG+CGG 0.568957 2:-17943694 None:intergenic
ATGTGAAGGATGCTCTTATG+AGG 0.569555 2:+17948646 MsaT007431.1:CDS
TTGGGTTGAACATGTGGAAG+TGG 0.571125 2:+17947175 MsaT007431.1:CDS
TGGTTAAACTCCACTTGACC+AGG 0.571210 2:-17947555 None:intergenic
TTCATGGTAATCTAACTATG+AGG 0.572450 2:+17945953 MsaT007431.1:CDS
AGATCCTACAAAGCGATCAC+AGG 0.573425 2:+17947725 MsaT007431.1:CDS
TGTTTCCAACCACGAGAAAG+AGG 0.576681 2:-17946835 None:intergenic
AGCTACTATTCTAAATGTGA+AGG 0.578993 2:+17948632 MsaT007431.1:CDS
AGACCATCAACATTTCGTGC+AGG 0.579781 2:+17945633 MsaT007431.1:CDS
AATCTCCAAATACTCAAGAT+GGG 0.583747 2:+17946103 MsaT007431.1:CDS
TGGCTGGCTTGTGTCATCGT+CGG 0.583839 2:-17947628 None:intergenic
GATGACACAAGCCAGCCAAA+TGG 0.584827 2:+17947633 MsaT007431.1:CDS
AAAAGTTGATGCTGGTGTAT+GGG 0.585328 2:+17946768 MsaT007431.1:CDS
AGCAGGCAGAGGGAGCCAAA+TGG 0.585985 2:-17947679 None:intergenic
AAATGTTGATGGTCTTCCAC+AGG 0.587353 2:-17945625 None:intergenic
GATTACTCAATGGGAGGTGG+CGG 0.589387 2:+17943600 MsaT007431.1:CDS
GCATCTGACAGTATTGAAGA+TGG 0.589389 2:+17948507 MsaT007431.1:CDS
CTATGAGGAGAGGTGTGCTA+GGG 0.589969 2:+17945968 MsaT007431.1:CDS
TTGGTCAACTGCATATGGAC+TGG 0.592188 2:-17948565 None:intergenic
TCTGCATGTAACTTGCAAGA+AGG 0.593980 2:-17945816 None:intergenic
TGAAATTCCACCAAACCGAA+CGG 0.595042 2:+17945909 MsaT007431.1:CDS
AAATGCTCAGCTGAAACAAG+AGG 0.596460 2:+17945669 MsaT007431.1:CDS
ATGGATTACTCAATGGGAGG+TGG 0.596619 2:+17943597 MsaT007431.1:CDS
GGTTCGCCTGGAAGTCGAGA+TGG 0.599887 2:+17946563 MsaT007431.1:CDS
ATGGTGCATTAAGATTTGGT+TGG 0.601403 2:-17948479 None:intergenic
ATCACCATTTGCCTCTGAGT+AGG 0.601759 2:-17948312 None:intergenic
AGATGGCTCGATGTTAACAT+TGG 0.603127 2:+17948524 MsaT007431.1:CDS
AACACTGCACCTCTTTCTCG+TGG 0.603163 2:+17946826 MsaT007431.1:CDS
ACTATGGATGTGGCTTTAAG+GGG 0.607798 2:+17948399 MsaT007431.1:CDS
CAGTGATCCAAACACTTGAA+GGG 0.609013 2:+17947469 MsaT007431.1:intron
CATTCGTGAAATGCCTCATG+GGG 0.609503 2:+17946873 MsaT007431.1:CDS
AAACTTTCTGAGCAGGCAGA+GGG 0.610006 2:-17947689 None:intergenic
AAGTATGGATGTTTGAGCGG+AGG 0.615778 2:-17943691 None:intergenic
TGGTCTTCCACAGGAAATGG+TGG 0.617088 2:-17945616 None:intergenic
TCAAGTTGTAGATGCTGCAA+TGG 0.619732 2:+17946035 MsaT007431.1:CDS
GCAGAAAGAGATAACACTAC+CGG 0.621791 2:+17945834 MsaT007431.1:CDS
GTTCTGCCTCGTCTGGATGG+TGG 0.624308 2:-17944840 None:intergenic
ATACCTGTTGAAGAAACCAT+AGG 0.625785 2:+17947326 MsaT007431.1:CDS
AGGCAGAGGGAGCCAAATGG+TGG 0.636325 2:-17947676 None:intergenic
AATTCGCGAGGCCTCACTAG+AGG 0.637219 2:-17946724 None:intergenic
CCTGTTGAAGAAACCATAGG+AGG 0.639101 2:+17947329 MsaT007431.1:CDS
TCACGAATGATGCATCCAGA+GGG 0.641603 2:-17946859 None:intergenic
ACAATTTGCGAAGCTTGTGG+GGG 0.652499 2:+17945592 MsaT007431.1:CDS
CTGGCTTGTGTCATCGTCGG+TGG 0.655813 2:-17947625 None:intergenic
TTCACGAATGATGCATCCAG+AGG 0.657086 2:-17946860 None:intergenic
GGTAATCTAACTATGAGGAG+AGG 0.673701 2:+17945958 MsaT007431.1:CDS
GGAATGCCACCATCCAGACG+AGG 0.674051 2:+17944834 MsaT007431.1:CDS
CGAATGATGTTAAATTCGCG+AGG 0.685235 2:-17946736 None:intergenic
TGATCCAAACACTTGAAGGG+AGG 0.696996 2:+17947472 MsaT007431.1:CDS
GAAATGGATTACTCAATGGG+AGG 0.699453 2:+17943594 None:intergenic
AGTCATTCATCCCTACTCAG+AGG 0.722493 2:+17948301 MsaT007431.1:intron
ATTCGTGAAATGCCTCATGG+GGG 0.745536 2:+17946874 MsaT007431.1:CDS
AGTTGACCAAGTGCTTAATG+TGG 0.750936 2:+17948578 MsaT007431.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 17943597 17948677 17943597 ID=MsaG007431
Chr2 mRNA 17943597 17948677 17943597 ID=MsaT007431.1;Parent=MsaG007431
Chr2 exon 17943597 17943718 17943597 ID=MsaT007431.1.exon1;Parent=MsaT007431.1
Chr2 CDS 17943597 17943718 17943597 ID=cds.MsaT007431.1;Parent=MsaT007431.1
Chr2 exon 17944825 17944942 17944825 ID=MsaT007431.1.exon2;Parent=MsaT007431.1
Chr2 CDS 17944825 17944942 17944825 ID=cds.MsaT007431.1;Parent=MsaT007431.1
Chr2 exon 17945499 17945690 17945499 ID=MsaT007431.1.exon3;Parent=MsaT007431.1
Chr2 CDS 17945499 17945690 17945499 ID=cds.MsaT007431.1;Parent=MsaT007431.1
Chr2 exon 17945799 17946269 17945799 ID=MsaT007431.1.exon4;Parent=MsaT007431.1
Chr2 CDS 17945799 17946269 17945799 ID=cds.MsaT007431.1;Parent=MsaT007431.1
Chr2 exon 17946497 17946598 17946497 ID=MsaT007431.1.exon5;Parent=MsaT007431.1
Chr2 CDS 17946497 17946598 17946497 ID=cds.MsaT007431.1;Parent=MsaT007431.1
Chr2 exon 17946697 17946903 17946697 ID=MsaT007431.1.exon6;Parent=MsaT007431.1
Chr2 CDS 17946697 17946903 17946697 ID=cds.MsaT007431.1;Parent=MsaT007431.1
Chr2 exon 17947170 17947350 17947170 ID=MsaT007431.1.exon7;Parent=MsaT007431.1
Chr2 CDS 17947170 17947350 17947170 ID=cds.MsaT007431.1;Parent=MsaT007431.1
Chr2 exon 17947472 17947746 17947472 ID=MsaT007431.1.exon8;Parent=MsaT007431.1
Chr2 CDS 17947472 17947746 17947472 ID=cds.MsaT007431.1;Parent=MsaT007431.1
Chr2 exon 17948069 17948158 17948069 ID=MsaT007431.1.exon9;Parent=MsaT007431.1
Chr2 CDS 17948069 17948158 17948069 ID=cds.MsaT007431.1;Parent=MsaT007431.1
Chr2 exon 17948303 17948677 17948303 ID=MsaT007431.1.exon10;Parent=MsaT007431.1
Chr2 CDS 17948303 17948677 17948303 ID=cds.MsaT007431.1;Parent=MsaT007431.1
Gene Sequence

>MsaT007431.1

ATGGATTACTCAATGGGAGGTGGCGGTGAGTCCGGTGATGAAGACATTAGTAACTTTCGAGATGATGGAAACAAACCTTCCTACAAACGTCTTACCTCCGCTCAAACATCCATACTTGAAAATTTCATGAAGGAATGCCACCATCCAGACGAGGCAGAACGACGTCAATTGGCTGTGAAAGTTGGGCTTGAGCCCAAACAAATCAAGTTTTGGTTTCAGAACAAGAGAACTCTGTTGAAGCATCAACATGAACGAGAGAAAAACGGTGCTCTTCGTCGGGAGAATGACAAGATTCGTAACGAAAATCTCAAAATAAAAGAGGTACTGAAAGCCACAATTTGCGAAGCTTGTGGGGGTCCACCATTTCCTGTGGAAGACCATCAACATTTCGTGCAGGAGATGCAGCAAGAAAATGCTCAGCTGAAACAAGAGTGTGAAAAAATGTCTAGCCTTCTTGCAAGTTACATGCAGAAAGAGATAACACTACCGGAGTTTGAGCAAGCTCTTAAGTCTATAAAATCATTTTCACGTGACAGAGAACTTGAAATTCCACCAAACCGAACGGTTTATGAGTGCTCAAATCATGTTCATGGTAATCTAACTATGAGGAGAGGTGTGCTAGGGCTCAACTTAACACAAAACTATGATGCACAAAAGATAACTATGTCTCAAGTTGTAGATGCTGCAATGGATGAATTGGTTAGGCTTGTGAGAGTTGATGAGCCTTTTTGGGTCAAATCTCCAAATACTCAAGATGGGTACACTCTTCATCGCGAAAGTTATGAGCAAGTTTTTCCAAAGAACAATCACTTTAAAGGTGCGTATGTGTGCGAAGAATCTTCCAAATATTCAGGATTGGTGAAGATTAGTGGCATAGATTTGGTTGGCATGTTTCTCGACTCGGTCAAATGGACAAACTTGTTCCCTACAATTGTTACCAAAGCTGAAACTATCAAAGTGTTTGAAATCGGTTCGCCTGGAAGTCGAGATGGAGCTTTATTACTGATGAATGAAGAAATGCATATTTTATCACCTCTAGTGAGGCCTCGCGAATTTAACATCATTCGCTACTGCAGAAAAGTTGATGCTGGTGTATGGGTGATAACTGATGTCTCATTTGACTCCTCTCGACCAAACACTGCACCTCTTTCTCGTGGTTGGAAACATCCCTCTGGATGCATCATTCGTGAAATGCCTCATGGGGGTTGTCTGGTCACTTGGGTTGAACATGTGGAAGTGGAAGATAAGATCCATACACATTACATGTATAGAGATCTTGTTGGCGACTATAATTTATACGGAGCTGAAAGCTGGATTAAGGAGCTTCAAAGAATGTGTGAGAGATCTTTAGGCTCTTATGTTAAAGCAATACCTGTTGAAGAAACCATAGGAGTGATCCAAACACTTGAAGGGAGGAATAGTGTGATAAAGCTTGCTGATAGAATGGTCAAAATGTTTTGTGAATGCTTAACAATGCCTGGTCAAGTGGAGTTTAACCATTTAACTCTTGATAGCATTGGTGGGGTTAGAGTCTCTCTTCGCAATACCACCGACGATGACACAAGCCAGCCAAATGGCACTGTTGTTACTGCTGCTACCACCATTTGGCTCCCTCTGCCTGCTCAGAAAGTTTTTGAATTCCTTAAAGATCCTACAAAGCGATCACAGTGGGATGGTCTTTCTTATGGAAATCCAATGCATGAAATTGCACACATTTCAAATGGACCCTATCATGGCAACTGCATTTCAATTATCAAGTCATTCATCCCTACTCAGAGGCAAATGGTGATTCTACAAGAAAGTTTCACTTCTCCTGTAGGATCATACATTATATATGCTCCAAATGATAGAAAAACTATGGATGTGGCTTTAAGGGGTGAAGACTGTAAAGAGTTACCAATCCTTCCAAATGGTTTTGTTATTTGTTCGAAGAGCCAACCAAATCTTAATGCACCATTTGGAGCATCTGACAGTATTGAAGATGGCTCGATGTTAACATTGGCAGCCCAAATTCTGAGCACCAGTCCATATGCAGTTGACCAAGTGCTTAATGTGGAAGATGTTACTTATATCAACACCCAATTGGCAGCTACTATTCTAAATGTGAAGGATGCTCTTATGAGGTCTATTTAA
Protein sequence

>MsaT007431.1

MDYSMGGGGESGDEDISNFRDDGNKPSYKRLTSAQTSILENFMKECHHPDEAERRQLAVKVGLEPKQIKFWFQNKRTLLKHQHEREKNGALRRENDKIRNENLKIKEVLKATICEACGGPPFPVEDHQHFVQEMQQENAQLKQECEKMSSLLASYMQKEITLPEFEQALKSIKSFSRDRELEIPPNRTVYECSNHVHGNLTMRRGVLGLNLTQNYDAQKITMSQVVDAAMDELVRLVRVDEPFWVKSPNTQDGYTLHRESYEQVFPKNNHFKGAYVCEESSKYSGLVKISGIDLVGMFLDSVKWTNLFPTIVTKAETIKVFEIGSPGSRDGALLLMNEEMHILSPLVRPREFNIIRYCRKVDAGVWVITDVSFDSSRPNTAPLSRGWKHPSGCIIREMPHGGCLVTWVEHVEVEDKIHTHYMYRDLVGDYNLYGAESWIKELQRMCERSLGSYVKAIPVEETIGVIQTLEGRNSVIKLADRMVKMFCECLTMPGQVEFNHLTLDSIGGVRVSLRNTTDDDTSQPNGTVVTAATTIWLPLPAQKVFEFLKDPTKRSQWDGLSYGNPMHEIAHISNGPYHGNCISIIKSFIPTQRQMVILQESFTSPVGSYIIYAPNDRKTMDVALRGEDCKELPILPNGFVICSKSQPNLNAPFGASDSIEDGSMLTLAAQILSTSPYAVDQVLNVEDVTYINTQLAATILNVKDALMRSI*