AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007434


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007434 MtrunA17_Chr2g0292781 80.682 88 14 1 66 153 2 86 3.77e-43 137
MsaG007434 MtrunA17_Chr4g0024351 31.818 154 92 4 10 150 59 212 1.65e-16 75.5
MsaG007434 MtrunA17_Chr7g0224481 33.884 121 69 4 17 131 22 137 4.28e-14 68.2
MsaG007434 MtrunA17_Chr7g0224451 35.537 121 67 5 17 131 22 137 6.07e-14 67.8
MsaG007434 MtrunA17_Chr2g0322731 32.117 137 81 3 15 139 104 240 8.01e-14 67.8
MsaG007434 MtrunA17_Chr1g0203671 29.932 147 89 4 15 148 99 244 1.59e-13 67.0
MsaG007434 MtrunA17_Chr2g0331581 31.148 122 73 3 15 125 143 264 5.96e-13 65.1
MsaG007434 MtrunA17_Chr6g0481041 33.588 131 71 4 15 131 33 161 6.19e-13 65.1
MsaG007434 MtrunA17_Chr6g0472471 30.328 122 74 3 15 126 73 193 7.34e-13 65.1
MsaG007434 MtrunA17_Chr5g0410711 31.405 121 73 2 15 125 93 213 1.39e-11 61.2
MsaG007434 MtrunA17_Chr5g0394111 27.737 137 78 4 18 136 40 173 7.25e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007434 AT1G05230.8 34.058 138 79 3 7 132 60 197 6.36e-16 74.3
MsaG007434 AT1G05230.4 34.058 138 79 3 7 132 60 197 6.36e-16 74.3
MsaG007434 AT1G05230.9 34.058 138 79 3 7 132 60 197 6.36e-16 74.3
MsaG007434 AT1G05230.10 34.058 138 79 3 7 132 60 197 6.36e-16 74.3
MsaG007434 AT1G05230.7 34.058 138 79 3 7 132 60 197 6.36e-16 74.3
MsaG007434 AT1G05230.11 34.058 138 79 3 7 132 60 197 6.36e-16 74.3
MsaG007434 AT1G05230.2 34.058 138 79 3 7 132 60 197 6.36e-16 74.3
MsaG007434 AT1G05230.6 34.058 138 79 3 7 132 60 197 6.36e-16 74.3
MsaG007434 AT1G05230.5 34.058 138 79 3 7 132 60 197 6.36e-16 74.3
MsaG007434 AT1G05230.1 34.058 138 79 3 7 132 60 197 6.36e-16 74.3
MsaG007434 AT1G05230.3 34.058 138 79 3 7 132 60 197 7.42e-16 73.9
MsaG007434 AT4G04890.2 30.323 155 94 4 9 149 60 214 1.70e-14 70.1
MsaG007434 AT4G04890.1 30.323 155 94 4 9 149 60 214 1.70e-14 70.1
MsaG007434 AT5G17320.1 35.246 122 69 3 14 125 29 150 4.51e-14 68.9
MsaG007434 AT1G05230.12 34.058 138 79 3 7 132 60 197 1.20e-13 67.8
MsaG007434 AT1G79840.5 34.211 114 71 2 15 125 105 217 2.84e-13 66.6
MsaG007434 AT1G79840.1 34.211 114 71 2 15 125 105 217 3.71e-13 66.2
MsaG007434 AT1G79840.3 34.211 114 71 2 15 125 47 159 3.92e-13 66.2
MsaG007434 AT1G79840.6 34.211 114 71 2 15 125 47 159 3.92e-13 66.2
MsaG007434 AT1G79840.2 34.211 114 71 2 15 125 134 246 4.26e-13 66.2
MsaG007434 AT1G79840.4 34.211 114 71 2 15 125 105 217 4.29e-13 65.9
MsaG007434 AT1G17920.2 32.520 123 70 3 15 126 25 145 7.40e-13 65.5
MsaG007434 AT1G17920.1 32.520 123 70 3 15 126 25 145 7.40e-13 65.5
MsaG007434 AT3G03260.1 33.058 121 69 3 17 126 29 148 1.19e-12 64.7
MsaG007434 AT3G03260.2 33.058 121 69 3 17 126 29 148 1.36e-12 64.7
MsaG007434 AT1G73360.1 32.520 123 70 3 15 126 36 156 1.76e-12 64.3
MsaG007434 AT4G17710.1 32.500 120 74 3 9 125 84 199 1.31e-11 61.6
MsaG007434 AT4G21750.2 30.882 136 82 3 9 132 60 195 1.32e-11 61.6
MsaG007434 AT4G21750.1 30.882 136 82 3 9 132 60 195 1.32e-11 61.6
MsaG007434 AT4G21750.3 30.882 136 82 3 9 132 60 195 1.32e-11 61.6
MsaG007434 AT4G21750.4 30.882 136 82 3 9 132 60 195 1.32e-11 61.6
MsaG007434 AT2G32370.1 30.612 147 86 5 11 147 68 208 1.38e-11 61.6
MsaG007434 AT5G46880.3 31.008 129 72 3 9 125 109 232 1.78e-11 61.2
MsaG007434 AT5G46880.1 31.008 129 72 3 9 125 109 232 1.78e-11 61.2
MsaG007434 AT5G46880.2 31.008 129 72 3 9 125 109 232 1.78e-11 61.2
MsaG007434 AT4G00730.2 32.450 151 85 4 9 142 132 282 3.96e-11 60.5
MsaG007434 AT4G00730.1 32.450 151 85 4 9 142 132 282 5.52e-11 60.1

Find 25 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCCATCTGATGATCTTTC+GGG 0.269210 2:-18004540 None:intergenic
AACCACCAGTTCACTTGTTT+TGG 0.280631 2:-18004029 None:intergenic
GGTTCCATCTGATGATCTTT+CGG 0.329348 2:-18004541 None:intergenic
AGCTGAAATTCAAATGCATT+TGG 0.349094 2:+18004221 MsaT007434.1:CDS
TCCTCTTCTCCCACGTAGCT+TGG 0.388842 2:-18004489 None:intergenic
AATGCATTTGGAAGCGAAAA+GGG 0.449227 2:+18004233 MsaT007434.1:CDS
AAATGCATTTGGAAGCGAAA+AGG 0.450705 2:+18004232 MsaT007434.1:CDS
ATTCTGCTGCTCAAAGATCT+AGG 0.453800 2:+18001866 MsaT007434.1:CDS
TTTGGAAGCGAAAAGGGAAA+TGG 0.469455 2:+18004239 MsaT007434.1:CDS
CTCTCACTGGAGTTGAAGTT+AGG 0.473633 2:+18004513 MsaT007434.1:CDS
ATACAAAAGAGCTCAAGTTA+AGG 0.476145 2:+18004055 MsaT007434.1:CDS
TTGAGCCAAAACAAGTGAAC+TGG 0.483755 2:+18004024 MsaT007434.1:CDS
TGGCTCAAGACCAAGCTCTT+CGG 0.488016 2:-18004009 None:intergenic
AACGTGAATCACGGCATGCT+TGG 0.497561 2:-18004284 None:intergenic
TCTCAAGAATGCTACTCAAA+AGG 0.498418 2:+18004179 MsaT007434.1:intron
GGGAGAAGAGGAACTCTCAC+TGG 0.520997 2:+18004500 MsaT007434.1:CDS
TCCAAGCTACGTGGGAGAAG+AGG 0.561213 2:+18004488 MsaT007434.1:CDS
AGTGAAATAGCCGAAGAGCT+TGG 0.563271 2:+18003999 MsaT007434.1:CDS
GTTGTTTCAAACGTGAATCA+CGG 0.584844 2:-18004293 None:intergenic
AGCCAAAACAAGTGAACTGG+TGG 0.610770 2:+18004027 MsaT007434.1:CDS
ATTCACGTTTGAAACAACTT+CGG 0.629375 2:+18004298 MsaT007434.1:CDS
AAAATTGAATCCAAGCTACG+TGG 0.657189 2:+18004479 MsaT007434.1:CDS
CTTCCCGAAAGATCATCAGA+TGG 0.663295 2:+18004537 MsaT007434.1:CDS
CTTTGAGCAGCAGAATGACG+AGG 0.674055 2:-18001859 None:intergenic
AAATTGAATCCAAGCTACGT+GGG 0.714001 2:+18004480 MsaT007434.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 18001814 18004572 18001814 ID=MsaG007434
Chr2 mRNA 18001814 18004572 18001814 ID=MsaT007434.1;Parent=MsaG007434
Chr2 exon 18001814 18001896 18001814 ID=MsaT007434.1.exon1;Parent=MsaT007434.1
Chr2 CDS 18001814 18001896 18001814 ID=cds.MsaT007434.1;Parent=MsaT007434.1
Chr2 exon 18003959 18004076 18003959 ID=MsaT007434.1.exon2;Parent=MsaT007434.1
Chr2 CDS 18003959 18004076 18003959 ID=cds.MsaT007434.1;Parent=MsaT007434.1
Chr2 exon 18004186 18004347 18004186 ID=MsaT007434.1.exon3;Parent=MsaT007434.1
Chr2 CDS 18004186 18004347 18004186 ID=cds.MsaT007434.1;Parent=MsaT007434.1
Chr2 exon 18004474 18004572 18004474 ID=MsaT007434.1.exon4;Parent=MsaT007434.1
Chr2 CDS 18004474 18004572 18004474 ID=cds.MsaT007434.1;Parent=MsaT007434.1
Gene Sequence

>MsaT007434.1

ATGGACTTAACTTTGAGATTAGCGCCACCTAAAAATGGAGCTTTTCCTCGTCATTCTGCTGCTCAAAGATCTAGGCTCGAAGAAATATTTCAAACTCTCAAATATCCAACTCATAAGCAAAAAAGTGAAATAGCCGAAGAGCTTGGTCTTGAGCCAAAACAAGTGAACTGGTGGTTTACATACAAAAGAGCTCAAGTTAAGAATGCTACTCAAAAGGAAGTTAACGCTGCTCTTCGAGCTGAAATTCAAATGCATTTGGAAGCGAAAAGGGAAATGGAGAGACAAAATAGTGTATCTTGCCAAGCATGCCGTGATTCACGTTTGAAACAACTTCGGCTTGAAAATGAACTTTTGAAAGAAAAGCTGTCAAAATTGAATCCAAGCTACGTGGGAGAAGAGGAACTCTCACTGGAGTTGAAGTTAGGTCTTCCCGAAAGATCATCAGATGGAACCTATCTTTAG

Protein sequence

>MsaT007434.1

MDLTLRLAPPKNGAFPRHSAAQRSRLEEIFQTLKYPTHKQKSEIAEELGLEPKQVNWWFTYKRAQVKNATQKEVNAALRAEIQMHLEAKREMERQNSVSCQACRDSRLKQLRLENELLKEKLSKLNPSYVGEEELSLELKLGLPERSSDGTYL*