Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG007520 | XP_024637264.1 | 92.683 | 82 | 6 | 0 | 27 | 108 | 84 | 165 | 3.33e-50 | 166 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsaG007520 | tr|G7JQ00|G7JQ00_MEDTR | 92.683 | 82 | 6 | 0 | 27 | 108 | 144 | 225 | 1.15e-49 | 166 |
| Gene ID | Type | Classification |
|---|---|---|
| MsaG007520 | TF | B3 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAACTTTAGTGATAAAATT+TGG | 0.119364 | 2:+19352345 | None:intergenic |
| TAACTTTAGTGATAAAATTT+GGG | 0.270703 | 2:+19352346 | None:intergenic |
| CACAGTGCATGTAGAAAATC+AGG | 0.323162 | 2:-19352310 | MsaT007520.1:intron |
| AGGAATTTGCCGAAAGTATC+AGG | 0.335653 | 2:+19351655 | None:intergenic |
| AATCGTGATGTTTCACTCTA+AGG | 0.341127 | 2:+19351635 | None:intergenic |
| ATAGGAAGATATGCGTTTAA+AGG | 0.361554 | 2:-19352379 | None:intergenic |
| TAGGAACTACCTGATACTTT+CGG | 0.400617 | 2:-19351664 | MsaT007520.1:CDS |
| TCCGAACATCACATTTGGTA+TGG | 0.417548 | 2:+19351549 | None:intergenic |
| CCGAACATCACATTTGGTAT+GGG | 0.451435 | 2:+19351550 | None:intergenic |
| TTGTGCTGCCAATGGTATTG+AGG | 0.463000 | 2:-19351509 | MsaT007520.1:CDS |
| CCAAATGTGATGTTCGGAAA+AGG | 0.475440 | 2:-19351544 | MsaT007520.1:CDS |
| CCCATACCAAATGTGATGTT+CGG | 0.477035 | 2:-19351550 | MsaT007520.1:CDS |
| GTCATGCGTTTATCAACGCT+TGG | 0.494559 | 2:+19351426 | None:intergenic |
| TGTGCTGCCAATGGTATTGA+GGG | 0.501205 | 2:-19351508 | MsaT007520.1:CDS |
| ACATGAATGATATTGTCATA+AGG | 0.526037 | 2:+19351595 | None:intergenic |
| AACTTTAGTGATAAAATTTG+GGG | 0.527049 | 2:+19352347 | None:intergenic |
| CGAACATCACATTTGGTATG+GGG | 0.552019 | 2:+19351551 | None:intergenic |
| TTTCAAGTGCAAGTGCGTCA+TGG | 0.567746 | 2:-19351470 | MsaT007520.1:CDS |
| GTCTCCGCCCTCAATACCAT+TGG | 0.581950 | 2:+19351501 | None:intergenic |
| CACGATTACGTCATGTTGTG+CGG | 0.590860 | 2:-19351619 | MsaT007520.1:CDS |
| ATGTGATGTTCGGAAAAGGT+TGG | 0.606400 | 2:-19351540 | MsaT007520.1:CDS |
| ACTTTAGTGATAAAATTTGG+GGG | 0.668220 | 2:+19352348 | None:intergenic |
| GCTGCCAATGGTATTGAGGG+CGG | 0.739556 | 2:-19351505 | MsaT007520.1:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 19351438 | 19352391 | 19351438 | ID=MsaG007520 |
| Chr2 | mRNA | 19351438 | 19352391 | 19351438 | ID=MsaT007520.1;Parent=MsaG007520 |
| Chr2 | exon | 19351438 | 19351683 | 19351438 | ID=MsaT007520.1.exon2;Parent=MsaT007520.1 |
| Chr2 | CDS | 19351438 | 19351683 | 19351438 | ID=cds.MsaT007520.1;Parent=MsaT007520.1 |
| Chr2 | exon | 19352311 | 19352391 | 19352311 | ID=MsaT007520.1.exon1;Parent=MsaT007520.1 |
| Chr2 | CDS | 19352311 | 19352391 | 19352311 | ID=cds.MsaT007520.1;Parent=MsaT007520.1 |
| Gene Sequence |
| Protein sequence |
