AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007531

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007531 MtrunA17_Chr2g0289181 43.348 233 52 3 166 318 1 233 1.99e-55 180
MsaG007531 MtrunA17_Chr1g0202461 33.793 290 118 11 4 253 79 334 4.31e-28 112
MsaG007531 MtrunA17_Chr2g0293901 83.544 79 13 0 1 79 1 79 1.82e-24 98.6
MsaG007531 MtrunA17_Chr8g0348311 47.000 100 50 2 156 255 134 230 3.10e-23 98.2
MsaG007531 MtrunA17_Chr7g0248691 46.602 103 52 2 153 255 130 229 5.77e-23 97.1
MsaG007531 MtrunA17_Chr6g0470151 41.270 126 58 2 78 202 243 353 2.28e-22 96.3
MsaG007531 MtrunA17_Chr8g0387501 41.228 114 65 2 180 293 130 241 1.43e-21 92.4
MsaG007531 MtrunA17_Chr6g0470161 40.367 109 42 1 210 318 35 120 1.17e-20 85.9
MsaG007531 MtrunA17_Chr2g0293481 39.669 121 61 3 144 253 221 340 1.25e-20 92.0
MsaG007531 MtrunA17_Chr4g0071521 45.783 83 42 1 157 236 220 302 9.38e-20 88.2
MsaG007531 MtrunA17_Chr7g0270051 31.818 198 105 5 130 307 3 190 1.36e-19 86.7
MsaG007531 MtrunA17_Chr8g0379461 46.429 84 38 1 179 262 76 152 1.36e-19 86.3
MsaG007531 MtrunA17_Chr4g0053281 42.593 108 55 1 136 236 216 323 1.56e-19 87.8
MsaG007531 MtrunA17_Chr5g0401821 45.783 83 42 1 157 236 174 256 1.80e-19 86.3
MsaG007531 MtrunA17_Chr2g0284321 35.714 154 90 5 151 297 2 153 3.22e-19 84.7
MsaG007531 MtrunA17_Chr8g0366921 56.250 80 28 2 1 79 1 74 9.05e-19 85.1
MsaG007531 MtrunA17_Chr8g0366921 45.238 84 44 2 78 160 238 320 5.50e-12 65.5
MsaG007531 MtrunA17_Chr2g0319961 57.377 61 26 0 179 239 80 140 2.20e-18 83.2
MsaG007531 MtrunA17_Chr1g0184691 45.783 83 44 1 156 238 271 352 3.13e-18 84.3
MsaG007531 MtrunA17_Chr7g0228941 50.769 65 32 0 173 237 46 110 7.50e-18 78.2
MsaG007531 MtrunA17_Chr1g0147881 43.210 81 45 1 156 236 240 319 1.28e-17 82.4
MsaG007531 MtrunA17_Chr7g0266621 52.500 80 37 1 80 158 223 302 2.48e-17 80.9
MsaG007531 MtrunA17_Chr7g0266621 56.962 79 31 1 1 79 1 76 1.22e-14 73.2
MsaG007531 MtrunA17_Chr4g0074001 46.250 80 41 2 161 239 340 418 6.01e-17 81.3
MsaG007531 MtrunA17_Chr3g0139021 43.750 80 44 1 158 236 292 371 6.06e-17 80.9
MsaG007531 MtrunA17_Chr7g0263531 52.632 76 34 2 175 250 185 258 6.87e-16 77.8
MsaG007531 MtrunA17_Chr7g0263531 43.750 64 35 1 176 239 358 420 3.98e-14 72.8
MsaG007531 MtrunA17_Chr7g0218081 47.222 72 37 1 168 239 399 469 1.08e-15 77.4
MsaG007531 MtrunA17_Chr7g0218081 46.970 66 33 2 175 240 224 287 4.29e-13 69.7
MsaG007531 MtrunA17_Chr5g0445311 43.056 72 40 1 168 239 353 423 2.12e-15 76.6
MsaG007531 MtrunA17_Chr5g0445311 44.444 72 38 2 178 249 199 268 4.13e-12 66.6
MsaG007531 MtrunA17_Chr3g0079691 41.758 91 49 3 178 267 279 366 6.39e-15 75.5
MsaG007531 MtrunA17_Chr3g0079691 35.354 99 52 2 152 239 457 554 1.45e-12 68.2
MsaG007531 MtrunA17_Chr3g0101131 41.667 72 41 1 168 239 404 474 9.56e-15 74.7
MsaG007531 MtrunA17_Chr3g0101131 45.946 74 38 2 175 248 239 310 6.22e-13 69.3
MsaG007531 MtrunA17_Chr5g0447701 45.833 72 38 1 168 239 241 311 1.34e-14 73.9
MsaG007531 MtrunA17_Chr3g0099641 31.724 145 87 3 120 264 203 335 1.41e-14 73.9
MsaG007531 MtrunA17_Chr5g0418191 38.889 90 54 1 168 257 309 397 3.27e-14 72.8
MsaG007531 MtrunA17_Chr5g0418191 49.231 65 31 2 175 239 147 209 8.53e-12 65.5
MsaG007531 MtrunA17_Chr2g0322391 36.134 119 70 3 146 264 321 433 3.32e-14 73.2
MsaG007531 MtrunA17_Chr2g0302391 44.615 65 36 0 179 243 211 275 3.78e-14 72.8
MsaG007531 MtrunA17_Chr8g0368721 46.429 84 44 1 78 160 229 312 4.88e-14 71.6
MsaG007531 MtrunA17_Chr8g0368721 53.165 79 33 1 1 79 1 75 2.47e-12 66.6
MsaG007531 MtrunA17_Chr4g0075201 27.429 175 108 4 81 239 156 327 6.54e-14 72.0
MsaG007531 MtrunA17_Chr5g0432161 48.333 60 30 1 180 239 422 480 7.65e-14 72.0
MsaG007531 MtrunA17_Chr5g0432161 52.381 63 28 2 178 240 243 303 8.01e-13 68.9
MsaG007531 MtrunA17_Chr7g0270191 36.170 94 56 3 149 239 112 204 1.14e-13 70.5
MsaG007531 MtrunA17_Chr3g0136961 45.614 57 31 0 180 236 139 195 1.37e-13 70.1
MsaG007531 MtrunA17_Chr1g0194481 36.667 90 55 2 159 248 139 226 1.76e-13 70.1
MsaG007531 MtrunA17_Chr1g0150271 28.395 162 99 4 85 241 14 163 1.87e-13 69.3
MsaG007531 MtrunA17_Chr4g0025061 36.145 83 53 0 174 256 355 437 2.37e-13 70.5
MsaG007531 MtrunA17_Chr7g0249311 31.092 119 67 3 140 244 339 456 2.96e-13 70.5
MsaG007531 MtrunA17_Chr7g0249311 45.205 73 38 2 178 250 178 248 5.31e-11 63.5
MsaG007531 MtrunA17_Chr3g0106491 46.667 60 31 1 180 239 366 424 3.12e-13 70.1
MsaG007531 MtrunA17_Chr3g0106491 51.613 62 28 2 178 239 197 256 2.55e-12 67.4
MsaG007531 MtrunA17_Chr4g0001361 42.188 64 37 0 180 243 163 226 3.18e-13 69.3
MsaG007531 MtrunA17_Chr4g0042831 43.750 64 35 1 176 239 275 337 4.84e-13 69.3
MsaG007531 MtrunA17_Chr4g0042831 33.010 103 61 2 141 243 114 208 4.53e-11 63.2
MsaG007531 MtrunA17_Chr7g0270831 38.462 78 47 1 162 239 200 276 4.95e-13 69.3
MsaG007531 MtrunA17_Chr7g0238921 45.333 75 37 2 177 251 122 192 7.14e-13 68.2
MsaG007531 MtrunA17_Chr2g0288871 35.000 100 61 3 141 239 269 365 7.64e-13 68.9
MsaG007531 MtrunA17_Chr4g0025051 36.145 83 53 0 174 256 330 412 1.14e-12 68.6
MsaG007531 MtrunA17_Chr7g0249301 47.541 61 29 3 180 239 293 351 1.67e-12 67.4
MsaG007531 MtrunA17_Chr1g0150281 43.860 57 32 0 180 236 141 197 3.00e-12 66.2
MsaG007531 MtrunA17_Chr7g0243891 47.143 70 35 2 171 240 208 275 3.60e-12 67.0
MsaG007531 MtrunA17_Chr7g0243891 38.889 72 43 1 168 239 363 433 9.39e-11 62.8
MsaG007531 MtrunA17_Chr4g0004911 42.424 66 37 1 174 239 331 395 3.70e-12 67.0
MsaG007531 MtrunA17_Chr4g0051181 27.647 170 102 4 70 239 155 303 3.73e-12 66.6
MsaG007531 MtrunA17_Chr4g0060271 38.462 78 46 1 164 239 238 315 7.32e-12 66.2
MsaG007531 MtrunA17_Chr4g0051881 41.429 70 39 2 175 244 175 242 7.97e-12 65.1
MsaG007531 MtrunA17_Chr2g0294231 45.000 60 32 1 180 239 204 262 8.98e-12 65.5
MsaG007531 MtrunA17_Chr7g0216891 31.858 113 72 3 124 236 169 276 1.04e-11 65.5
MsaG007531 MtrunA17_Chr2g0333851 37.805 82 47 1 164 241 150 231 1.37e-11 64.3
MsaG007531 MtrunA17_Chr1g0150801 44.262 61 33 1 176 236 99 158 1.43e-11 62.0
MsaG007531 MtrunA17_Chr5g0418781 34.314 102 65 2 135 236 81 180 1.97e-11 63.5
MsaG007531 MtrunA17_Chr5g0443131 41.538 65 37 1 180 244 158 221 2.09e-11 63.9
MsaG007531 MtrunA17_Chr7g0266611 63.077 65 23 1 254 318 102 165 3.00e-11 61.2
MsaG007531 MtrunA17_Chr4g0068541 37.681 69 42 1 180 248 209 276 3.41e-11 63.5
MsaG007531 MtrunA17_Chr3g0129321 40.625 64 37 1 176 239 115 177 4.37e-11 61.6
MsaG007531 MtrunA17_Chr1g0188941 32.184 87 54 2 153 239 29 110 4.67e-11 59.3
MsaG007531 MtrunA17_Chr8g0383251 37.349 83 49 2 159 239 86 167 8.61e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007531 AT4G01250.1 29.814 322 167 12 4 317 3 273 5.61e-30 115
MsaG007531 AT5G45050.4 38.333 120 72 2 156 274 1017 1135 1.37e-19 90.1
MsaG007531 AT5G45050.1 38.333 120 72 2 156 274 1156 1274 1.39e-19 90.1
MsaG007531 AT5G45050.2 38.333 120 72 2 156 274 1128 1246 1.60e-19 89.7
MsaG007531 AT2G34830.1 42.308 104 49 3 154 248 182 283 3.48e-19 87.8
MsaG007531 AT2G34830.2 42.308 104 49 3 154 248 191 292 3.65e-19 87.8
MsaG007531 AT4G23550.1 37.984 129 67 4 163 279 124 251 3.91e-19 86.7
MsaG007531 AT2G24570.1 45.783 83 44 1 156 238 221 302 7.67e-19 85.9
MsaG007531 AT5G28650.1 47.059 85 42 1 157 238 237 321 9.63e-19 85.9
MsaG007531 AT4G31550.2 46.341 82 41 1 158 236 221 302 1.00e-18 85.5
MsaG007531 AT4G31550.1 45.679 81 43 1 156 236 224 303 1.16e-18 85.5
MsaG007531 AT1G29280.1 45.055 91 41 1 158 239 44 134 1.18e-18 84.3
MsaG007531 AT1G30650.1 42.308 104 49 3 154 248 184 285 1.21e-18 86.3
MsaG007531 AT3G04670.1 47.059 85 42 1 157 238 237 321 1.89e-18 84.7
MsaG007531 AT2G23320.1 57.895 57 24 0 180 236 241 297 2.88e-18 84.3
MsaG007531 AT4G24240.1 45.783 83 42 1 157 236 256 338 5.12e-18 84.0
MsaG007531 AT3G58710.2 51.515 66 32 0 179 244 70 135 2.20e-17 80.9
MsaG007531 AT3G58710.1 51.515 66 32 0 179 244 71 136 2.35e-17 80.9
MsaG007531 AT4G30935.1 40.000 105 58 3 136 239 290 390 8.39e-17 81.3
MsaG007531 AT2G30590.1 43.529 85 45 1 157 238 288 372 1.56e-16 79.7
MsaG007531 AT5G07100.1 33.333 144 88 5 107 247 163 301 4.60e-16 77.8
MsaG007531 AT5G07100.1 43.056 72 40 1 172 243 110 180 7.40e-12 65.5
MsaG007531 AT5G07100.2 33.333 144 88 5 107 247 70 208 4.83e-16 76.3
MsaG007531 AT5G07100.2 43.056 72 40 1 172 243 17 87 1.12e-11 63.9
MsaG007531 AT5G07100.4 33.333 144 88 5 107 247 70 208 4.83e-16 76.3
MsaG007531 AT5G07100.4 43.056 72 40 1 172 243 17 87 1.12e-11 63.9
MsaG007531 AT5G07100.5 33.333 144 88 5 107 247 70 208 4.83e-16 76.3
MsaG007531 AT5G07100.5 43.056 72 40 1 172 243 17 87 1.12e-11 63.9
MsaG007531 AT5G07100.3 33.333 144 88 5 107 247 70 208 4.83e-16 76.3
MsaG007531 AT5G07100.3 43.056 72 40 1 172 243 17 87 1.12e-11 63.9
MsaG007531 AT2G04880.2 36.434 129 62 5 141 255 231 353 8.06e-16 78.2
MsaG007531 AT2G04880.1 36.434 129 62 5 141 255 255 377 8.99e-16 78.2
MsaG007531 AT3G01080.2 48.485 66 33 1 174 239 339 403 1.59e-15 77.4
MsaG007531 AT3G01080.2 45.714 70 36 2 175 244 201 268 1.59e-13 71.2
MsaG007531 AT3G01080.1 48.485 66 33 1 174 239 301 365 2.03e-15 77.0
MsaG007531 AT3G01080.1 48.485 66 32 2 175 240 163 226 2.05e-13 70.9
MsaG007531 AT4G26640.5 41.667 72 41 1 168 239 194 264 1.16e-14 74.3
MsaG007531 AT4G26640.5 34.454 119 68 3 138 256 2 110 1.70e-11 65.1
MsaG007531 AT4G26640.6 41.667 72 41 1 168 239 194 264 1.16e-14 74.3
MsaG007531 AT4G26640.6 34.454 119 68 3 138 256 2 110 1.70e-11 65.1
MsaG007531 AT4G26640.4 41.667 72 41 1 168 239 194 264 1.16e-14 74.3
MsaG007531 AT4G26640.4 34.454 119 68 3 138 256 2 110 1.70e-11 65.1
MsaG007531 AT4G26640.3 41.667 72 41 1 168 239 194 264 1.16e-14 74.3
MsaG007531 AT4G26640.3 34.454 119 68 3 138 256 2 110 1.70e-11 65.1
MsaG007531 AT4G26640.7 41.667 72 41 1 168 239 330 400 1.24e-14 74.7
MsaG007531 AT4G26640.7 34.146 123 71 3 134 256 134 246 1.10e-11 65.9
MsaG007531 AT4G26640.2 41.667 72 41 1 168 239 370 440 1.35e-14 74.7
MsaG007531 AT4G26640.2 34.146 123 71 3 134 256 174 286 1.09e-11 65.9
MsaG007531 AT1G69810.2 43.590 78 43 1 174 250 140 217 1.44e-14 73.6
MsaG007531 AT4G26640.1 41.667 72 41 1 168 239 298 368 1.50e-14 74.3
MsaG007531 AT4G26640.1 34.146 123 71 3 134 256 102 214 1.28e-11 65.5
MsaG007531 AT1G69810.1 43.590 78 43 1 174 250 198 275 1.81e-14 73.9
MsaG007531 AT1G13960.1 41.667 72 41 1 168 239 398 468 4.16e-14 73.2
MsaG007531 AT1G13960.1 44.928 69 36 2 175 243 225 291 3.30e-12 67.4
MsaG007531 AT2G03340.1 41.667 72 41 1 168 239 404 474 4.38e-14 73.2
MsaG007531 AT2G03340.1 45.714 70 36 2 175 244 246 313 7.98e-12 66.2
MsaG007531 AT2G37260.1 41.463 82 43 2 159 236 325 405 5.22e-14 72.8
MsaG007531 AT2G37260.1 50.769 65 30 2 175 239 161 223 5.32e-12 66.6
MsaG007531 AT2G38470.1 51.471 68 31 2 173 240 178 243 5.99e-14 72.8
MsaG007531 AT2G38470.1 46.667 60 31 1 180 239 363 421 1.08e-13 72.0
MsaG007531 AT2G37260.3 41.463 82 43 2 159 236 245 325 7.05e-14 72.0
MsaG007531 AT2G37260.3 50.769 65 30 2 175 239 81 143 8.01e-12 65.9
MsaG007531 AT2G37260.2 41.463 82 43 2 159 236 243 323 7.51e-14 71.6
MsaG007531 AT2G37260.2 50.769 65 30 2 175 239 79 141 8.79e-12 65.5
MsaG007531 AT3G01080.3 45.714 70 36 2 175 244 201 268 8.36e-14 71.2
MsaG007531 AT1G13960.2 41.667 72 41 1 168 239 245 315 8.51e-14 71.6
MsaG007531 AT1G13960.2 44.928 69 36 2 175 243 72 138 7.28e-12 65.9
MsaG007531 AT4G04450.2 36.145 83 53 0 174 256 193 275 1.15e-13 71.6
MsaG007531 AT4G04450.1 36.145 83 53 0 174 256 287 369 2.01e-13 71.2
MsaG007531 AT5G26170.1 47.619 63 32 1 174 236 108 169 3.23e-13 67.4
MsaG007531 AT4G22070.4 39.394 66 40 0 174 239 292 357 6.45e-13 69.7
MsaG007531 AT4G22070.3 39.394 66 40 0 174 239 292 357 6.45e-13 69.7
MsaG007531 AT4G22070.2 39.394 66 40 0 174 239 292 357 6.45e-13 69.7
MsaG007531 AT4G22070.1 39.394 66 40 0 174 239 292 357 6.45e-13 69.7
MsaG007531 AT2G30250.1 45.312 64 34 1 180 243 329 391 7.88e-13 68.9
MsaG007531 AT1G80840.1 37.805 82 47 1 165 242 128 209 8.31e-13 68.2
MsaG007531 AT4G26440.1 38.542 96 53 3 167 262 170 259 8.93e-13 69.3
MsaG007531 AT2G47260.1 36.667 90 55 2 150 239 146 233 9.03e-13 68.6
MsaG007531 AT1G62300.1 39.394 66 40 0 174 239 307 372 1.86e-12 68.2
MsaG007531 AT4G18170.1 36.047 86 53 2 159 244 153 236 5.30e-12 66.2
MsaG007531 AT1G69310.4 33.000 100 65 2 138 237 107 204 6.43e-12 65.5
MsaG007531 AT1G69310.2 33.000 100 65 2 138 237 107 204 6.43e-12 65.5
MsaG007531 AT1G69310.1 33.000 100 65 2 138 237 107 204 6.43e-12 65.5
MsaG007531 AT5G43290.1 54.386 57 25 1 180 236 115 170 7.05e-12 65.1
MsaG007531 AT5G56270.1 43.333 60 33 1 180 239 488 546 8.24e-12 66.2
MsaG007531 AT5G56270.1 45.588 68 35 2 172 239 266 331 1.33e-11 65.9
MsaG007531 AT1G29860.1 37.805 82 48 2 169 248 124 204 8.41e-12 65.1
MsaG007531 AT1G55600.1 31.461 89 60 1 151 239 279 366 1.00e-11 65.9
MsaG007531 AT4G31800.2 40.000 60 36 0 180 239 112 171 1.19e-11 64.3
MsaG007531 AT4G31800.1 40.000 60 36 0 180 239 177 236 1.52e-11 64.7
MsaG007531 AT3G62340.1 40.000 85 47 3 156 239 96 177 2.03e-11 63.9
MsaG007531 AT1G18860.1 43.333 60 34 0 180 239 240 299 2.87e-11 64.7
MsaG007531 AT5G49520.1 43.333 60 33 1 180 239 222 280 3.50e-11 63.9
MsaG007531 AT5G15130.2 37.662 77 46 1 165 239 169 245 3.78e-11 64.3
MsaG007531 AT5G15130.1 37.662 77 46 1 165 239 211 287 4.80e-11 63.9
MsaG007531 AT1G66550.2 33.333 153 79 7 156 294 78 221 6.83e-11 62.0
MsaG007531 AT5G45260.1 45.455 66 34 2 180 243 1209 1274 7.80e-11 63.5
MsaG007531 AT5G41570.1 31.183 93 61 2 146 236 63 154 8.49e-11 60.5
MsaG007531 AT1G66550.1 29.744 195 107 8 114 294 48 226 8.77e-11 61.6

Find 54 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCTGAAAGAGAATTTAT+TGG 0.190862 2:-19535477 MsaT007531.1:CDS
TAGAAACCCAAACTAGTTCT+AGG 0.250090 2:-19537057 MsaT007531.1:CDS
ACAAAGTTCTTAATTTCTTC+TGG 0.267372 2:+19535591 None:intergenic
GTAAAACCCTAGAACTAGTT+TGG 0.270284 2:+19537050 None:intergenic
CTAGTTTGGGTTTCTAGAAT+TGG 0.312695 2:+19537064 None:intergenic
CAAACCTCAGATCAAGAAAA+AGG 0.317679 2:-19537006 MsaT007531.1:intron
CTTCAATGGATAATGATGAT+TGG 0.367851 2:-19537921 None:intergenic
TTCAATGGATAATGATGATT+GGG 0.373937 2:-19537920 None:intergenic
GCTTATATTGTGACGTATAA+AGG 0.379319 2:-19535633 MsaT007531.1:CDS
GTGGTGGTGGTTAGGATTTG+AGG 0.379807 2:+19537820 None:intergenic
GATTGTTGCTGATTTGTGTT+TGG 0.383802 2:+19537028 None:intergenic
TCTAACTCTATGAAGTCATT+TGG 0.386725 2:+19537697 None:intergenic
TACTGATGGTGGTGGTGGTT+AGG 0.393621 2:+19537812 None:intergenic
TCTGACATAGCTGTTATGTT+TGG 0.394725 2:+19535423 None:intergenic
TAAAACCCTAGAACTAGTTT+GGG 0.406708 2:+19537051 None:intergenic
GAAATTAAGAACTTTGTTGT+TGG 0.434538 2:-19535585 MsaT007531.1:CDS
TATGTCAGAAGATTTATTGA+TGG 0.435423 2:-19535409 MsaT007531.1:CDS
TGGAGTTAGAGTTTCAGAAT+TGG 0.449537 2:+19537843 None:intergenic
TGTGTATGTTGTGGACTAGA+TGG 0.450832 2:+19537103 None:intergenic
GACTTTAACCACCTCAATAG+TGG 0.457259 2:-19535387 MsaT007531.1:CDS
ACAAGAGATGTTTGATTCAT+CGG 0.457654 2:+19535497 None:intergenic
ACCTGAGAATTGCTAGTCTC+TGG 0.471222 2:+19535525 None:intergenic
GCCAGAGACTAGCAATTCTC+AGG 0.477878 2:-19535526 MsaT007531.1:CDS
TGAAGTCATTTGGTTTAAGC+GGG 0.482952 2:+19537707 None:intergenic
AGCGGTTGTGATTTCAATGC+AGG 0.486927 2:+19537150 None:intergenic
ATGAAGTCATTTGGTTTAAG+CGG 0.492100 2:+19537706 None:intergenic
ATAACACACCAAGGACATCT+TGG 0.504754 2:+19536841 None:intergenic
TTGCTTCTTTGTGTATGTTG+TGG 0.506190 2:+19537094 None:intergenic
AGAAACCCAAACTAGTTCTA+GGG 0.507577 2:-19537056 MsaT007531.1:CDS
TGTCCTTGGTGTGTTATATG+AGG 0.509380 2:-19536835 MsaT007531.1:CDS
AGTTAGAGTTTCAGAATTGG+TGG 0.535695 2:+19537846 None:intergenic
GGAATAAAGCACCACTATTG+AGG 0.537769 2:+19535376 None:intergenic
GATGTTACTGATGGTGGTGG+TGG 0.548240 2:+19537807 None:intergenic
GTGGTGGTTAGGATTTGAGG+TGG 0.552967 2:+19537823 None:intergenic
AAAGAATGCCAAGATGTCCT+TGG 0.553216 2:-19536849 MsaT007531.1:CDS
GGATATGATGTTACTGATGG+TGG 0.569473 2:+19537801 None:intergenic
TGAACTTTCAACAGACTCAT+GGG 0.572196 2:-19536807 MsaT007531.1:CDS
GGTGGCAGAAGTAGTAAAGG+TGG 0.572647 2:+19537864 None:intergenic
TTAAAGGGTCTCCACATCCA+AGG 0.575832 2:-19536757 MsaT007531.1:intron
ATTGGTGGCAGAAGTAGTAA+AGG 0.577716 2:+19537861 None:intergenic
ATGTATGAAAACCTTGGATG+TGG 0.583670 2:+19536746 None:intergenic
ATGAACTTTCAACAGACTCA+TGG 0.586902 2:-19536808 MsaT007531.1:CDS
TCATGGGCATGGAGTAAACA+TGG 0.611520 2:-19536791 MsaT007531.1:CDS
TTTCAACAGACTCATGGGCA+TGG 0.620196 2:-19536802 MsaT007531.1:CDS
ATCATCATAGCATGATGATG+TGG 0.621279 2:+19537768 None:intergenic
TATGTTGTGGACTAGATGGA+TGG 0.621555 2:+19537107 None:intergenic
TGATGATGTGGTACTTTGAG+AGG 0.630706 2:+19537780 None:intergenic
TATGATGTTACTGATGGTGG+TGG 0.635627 2:+19537804 None:intergenic
AGAGGATATGATGTTACTGA+TGG 0.644683 2:+19537798 None:intergenic
TTCCCTCATATAACACACCA+AGG 0.662868 2:+19536832 None:intergenic
ATAAAGCACCACTATTGAGG+TGG 0.665488 2:+19535379 None:intergenic
GAAGTCATTTGGTTTAAGCG+GGG 0.666201 2:+19537708 None:intergenic
GTCCTTGGTGTGTTATATGA+GGG 0.673519 2:-19536834 MsaT007531.1:CDS
GTATGCACGATCAAAATGAG+CGG 0.723045 2:+19537132 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 19535371 19537938 19535371 ID=MsaG007531
Chr2 mRNA 19535371 19537938 19535371 ID=MsaT007531.1;Parent=MsaG007531
Chr2 exon 19535371 19535731 19535371 ID=MsaT007531.1.exon4;Parent=MsaT007531.1
Chr2 CDS 19535371 19535731 19535371 ID=cds.MsaT007531.1;Parent=MsaT007531.1
Chr2 exon 19536758 19536874 19536758 ID=MsaT007531.1.exon3;Parent=MsaT007531.1
Chr2 CDS 19536758 19536874 19536758 ID=cds.MsaT007531.1;Parent=MsaT007531.1
Chr2 exon 19537007 19537249 19537007 ID=MsaT007531.1.exon2;Parent=MsaT007531.1
Chr2 CDS 19537007 19537249 19537007 ID=cds.MsaT007531.1;Parent=MsaT007531.1
Chr2 exon 19537703 19537938 19537703 ID=MsaT007531.1.exon1;Parent=MsaT007531.1
Chr2 CDS 19537703 19537938 19537703 ID=cds.MsaT007531.1;Parent=MsaT007531.1
Gene Sequence

>MsaT007531.1

ATGGATAATGATGATTGGGATCTCTTTTCTATTGTGCGTAGCTGCAACACTACCACCTTTACTACTTCTGCCACCAATTCTGAAACTCTAACTCCACCTCAAATCCTAACCACCACCACCATCAGTAACATCATATCCTCTCAAAGTACCACATCATCATGCTATGATGATTTTACTTTTATCCAAGAAAATAATGCAATCTCTTTTGCCCCGCTTAAACCAAATGACTTCATAGATGATTTTTCAACACTCGTTGAACAACAACATATTCATCAAAGCAACAACAATCAAGTGTTTGTTTCGAAACATGTTGCCTGCATTGAAATCACAACCGCTCATTTTGATCGTGCATACAGCCATCCATCTAGTCCACAACATACACAAAGAAGCAATCAACTTCCAATTCTAGAAACCCAAACTAGTTCTAGGGTTTTACCAAACACAAATCAGCAACAATCAAACCTCAGATCAAGAAAAAGAAAAAAGAATGCCAAGATGTCCTTGGTGTGTTATATGAGGGAAGATGAACTTTCAACAGACTCATGGGCATGGAGTAAACATGGACAAAAATTCATTAAAGGGTCTCCACATCCAAGGTGCTATTATAAGTGCAGCAGTTTCGCAAATTGTCCAGCAAAAAAATATGTAGAGAAAAGCAAAACTCATGAGAACGCTTATATTGTGACGTATAAAGGAGAGCATGATCACCAAAAACCAGAAGAAATTAAGAACTTTGTTGTTGGTACCTCACATAACAAAATGATGCTGAAGTTTGATGAGCCAGAGACTAGCAATTCTCAGGTTTTTTCCGATGAATCAAACATCTCTTGTTCTGAAAGAGAATTTATTGGAAACTCTAATGATGATGATGACATTTTGATACCAAACATAACAGCTATGTCAGAAGATTTATTGATGGACTTTAACCACCTCAATAGTGGTGCTTTATTCCCATAG
Protein sequence

>MsaT007531.1

MDNDDWDLFSIVRSCNTTTFTTSATNSETLTPPQILTTTTISNIISSQSTTSSCYDDFTFIQENNAISFAPLKPNDFIDDFSTLVEQQHIHQSNNNQVFVSKHVACIEITTAHFDRAYSHPSSPQHTQRSNQLPILETQTSSRVLPNTNQQQSNLRSRKRKKNAKMSLVCYMREDELSTDSWAWSKHGQKFIKGSPHPRCYYKCSSFANCPAKKYVEKSKTHENAYIVTYKGEHDHQKPEEIKNFVVGTSHNKMMLKFDEPETSNSQVFSDESNISCSEREFIGNSNDDDDILIPNITAMSEDLLMDFNHLNSGALFP*