AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007608

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007608 MtrunA17_Chr2g0294671 96.646 477 16 0 1 477 20 496 0.0 948
MsaG007608 MtrunA17_Chr6g0448821 30.078 256 174 3 212 463 102 356 4.09e-31 123
MsaG007608 MtrunA17_Chr5g0417641 35.096 208 129 5 262 464 87 293 1.04e-29 118
MsaG007608 MtrunA17_Chr5g0428941 29.455 275 184 6 198 465 213 484 1.74e-29 121
MsaG007608 MtrunA17_Chr5g0428941 23.050 282 168 6 192 469 139 375 3.30e-13 71.6
MsaG007608 MtrunA17_Chr1g0212691 29.474 285 189 6 192 468 175 455 5.61e-29 119
MsaG007608 MtrunA17_Chr1g0212691 23.485 264 176 5 170 411 67 326 1.10e-13 73.2
MsaG007608 MtrunA17_Chr4g0034981 24.913 289 208 7 192 473 309 595 1.11e-26 113
MsaG007608 MtrunA17_Chr4g0034981 25.292 257 177 6 225 471 271 522 3.63e-20 93.6
MsaG007608 MtrunA17_Chr4g0034981 29.949 197 135 3 189 383 378 573 8.17e-15 77.0
MsaG007608 MtrunA17_Chr4g0043941 26.103 272 195 4 198 463 47 318 1.53e-26 109
MsaG007608 MtrunA17_Chr4g0043941 24.424 217 153 3 268 476 10 223 9.78e-14 72.4
MsaG007608 MtrunA17_Chr4g0043941 28.947 152 106 2 188 337 109 260 1.37e-12 68.9
MsaG007608 MtrunA17_Chr2g0304101 30.992 242 152 6 242 469 70 310 2.54e-26 108
MsaG007608 MtrunA17_Chr5g0445491 31.579 228 141 6 196 414 230 451 1.96e-25 108
MsaG007608 MtrunA17_Chr5g0445491 27.468 233 139 7 251 459 177 403 1.62e-17 85.1
MsaG007608 MtrunA17_Chr5g0445491 30.052 193 130 4 188 376 258 449 3.93e-17 84.0
MsaG007608 MtrunA17_Chr2g0311181 29.778 225 146 5 248 467 86 303 1.14e-22 98.6
MsaG007608 MtrunA17_Chr8g0391881 27.907 258 174 6 191 440 121 374 1.52e-20 93.2
MsaG007608 MtrunA17_Chr3g0127891 25.000 324 191 12 196 475 187 502 2.08e-20 94.0
MsaG007608 MtrunA17_Chr7g0250261 29.756 205 132 5 268 463 78 279 3.48e-16 79.0
MsaG007608 MtrunA17_Chr0c29g0494001 29.756 205 132 5 268 463 78 279 3.48e-16 79.0
MsaG007608 MtrunA17_Chr4g0067141 25.000 276 181 8 196 460 81 341 8.10e-13 69.7
MsaG007608 MtrunA17_Chr2g0296171 25.498 251 174 7 196 439 99 343 3.98e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007608 AT4G14605.2 63.901 446 150 4 29 464 24 468 0.0 560
MsaG007608 AT4G14605.1 63.901 446 150 4 29 464 24 468 0.0 560
MsaG007608 AT2G21710.1 28.912 294 194 7 192 475 316 604 3.45e-33 133
MsaG007608 AT2G21710.1 30.366 191 130 3 189 377 385 574 1.79e-13 73.2
MsaG007608 AT2G21710.1 28.114 281 182 7 58 337 306 567 4.34e-13 72.0
MsaG007608 AT2G44020.1 27.726 321 186 6 191 468 143 460 1.11e-29 122
MsaG007608 AT4G38160.4 27.372 274 186 5 202 465 52 322 2.40e-27 112
MsaG007608 AT4G38160.4 30.719 153 103 3 188 337 110 262 3.48e-14 73.9
MsaG007608 AT4G38160.1 27.372 274 186 5 202 465 52 322 2.40e-27 112
MsaG007608 AT4G38160.1 30.719 153 103 3 188 337 110 262 3.48e-14 73.9
MsaG007608 AT1G78930.1 28.125 256 179 3 212 463 320 574 3.30e-27 115
MsaG007608 AT4G38160.2 27.372 274 186 5 202 465 52 322 5.83e-27 112
MsaG007608 AT4G38160.2 30.719 153 103 3 188 337 110 262 4.73e-14 73.9
MsaG007608 AT4G38160.3 27.372 274 186 5 202 465 52 322 6.24e-27 112
MsaG007608 AT4G38160.3 30.719 153 103 3 188 337 110 262 5.36e-14 73.9
MsaG007608 AT2G34620.1 30.833 240 151 6 242 467 56 294 3.22e-25 105
MsaG007608 AT2G34620.1 28.972 214 143 7 170 379 66 274 2.29e-11 65.1
MsaG007608 AT2G21710.2 28.049 246 162 7 192 427 316 556 6.15e-24 105
MsaG007608 AT2G21710.2 25.229 218 156 2 228 438 245 462 3.82e-15 78.2
MsaG007608 AT2G21710.2 28.685 251 162 6 58 307 306 540 6.78e-13 71.2
MsaG007608 AT2G21710.2 31.847 157 104 3 189 343 385 540 4.23e-12 68.9
MsaG007608 AT2G03050.1 32.039 206 129 6 270 467 70 272 6.33e-24 101
MsaG007608 AT2G03050.1 25.346 217 140 8 168 379 53 252 5.10e-11 63.9
MsaG007608 AT2G03050.2 32.039 206 129 6 270 467 71 273 6.84e-24 101
MsaG007608 AT2G03050.2 25.346 217 140 8 168 379 54 253 5.55e-11 63.9
MsaG007608 AT2G21710.3 28.163 245 161 7 192 426 316 555 1.37e-23 104
MsaG007608 AT2G21710.3 25.229 218 156 2 228 438 245 462 3.07e-15 78.6
MsaG007608 AT2G21710.3 28.685 251 162 6 58 307 306 540 7.95e-13 71.2
MsaG007608 AT2G21710.3 31.847 157 104 3 189 343 385 540 3.98e-12 68.9
MsaG007608 AT4G02990.2 26.224 286 193 7 191 465 214 492 6.82e-23 102
MsaG007608 AT4G02990.2 25.909 220 145 4 170 375 115 330 1.00e-14 77.0
MsaG007608 AT4G02990.1 26.224 286 193 7 191 465 214 492 6.82e-23 102
MsaG007608 AT4G02990.1 25.909 220 145 4 170 375 115 330 1.00e-14 77.0
MsaG007608 AT3G18870.1 27.962 211 128 5 267 463 68 268 3.33e-20 90.9
MsaG007608 AT5G55580.1 25.941 239 162 7 196 422 223 458 3.95e-19 90.5
MsaG007608 AT5G55580.1 28.497 193 135 3 188 377 251 443 4.44e-18 87.0
MsaG007608 AT5G55580.1 23.864 264 188 8 185 440 180 438 3.35e-16 81.3
MsaG007608 AT5G55580.1 26.267 217 131 7 266 459 186 396 7.02e-13 71.2
MsaG007608 AT5G55580.1 25.000 188 131 5 268 445 223 410 1.64e-12 70.1
MsaG007608 AT5G55580.2 25.941 239 162 7 196 422 117 352 4.84e-19 89.4
MsaG007608 AT5G55580.2 28.497 193 135 3 188 377 145 337 3.72e-18 86.7
MsaG007608 AT5G55580.2 23.529 255 185 7 194 440 80 332 3.65e-16 80.5
MsaG007608 AT5G55580.2 25.778 225 138 7 258 459 72 290 5.09e-13 70.9
MsaG007608 AT5G55580.2 25.000 188 131 5 268 445 117 304 1.44e-12 69.7
MsaG007608 AT2G36000.1 29.082 196 126 5 279 467 122 311 1.04e-17 84.7
MsaG007608 AT2G36000.2 26.840 231 153 7 246 467 88 311 1.36e-17 84.0
MsaG007608 AT5G55580.3 27.097 155 110 3 196 347 223 377 2.18e-14 75.1
MsaG007608 AT5G55580.3 24.747 198 128 6 266 448 186 377 3.48e-12 68.6
MsaG007608 AT5G54180.1 24.555 281 171 9 189 465 247 490 1.41e-13 73.2
MsaG007608 AT1G79220.1 30.769 143 92 3 192 332 149 286 1.25e-11 66.6
MsaG007608 AT1G21150.3 24.815 270 181 7 196 456 56 312 1.82e-11 65.9
MsaG007608 AT1G21150.1 24.815 270 181 7 196 456 92 348 3.01e-11 65.5
MsaG007608 AT1G21150.2 24.815 270 181 7 196 456 156 412 6.09e-11 64.7
MsaG007608 AT1G62110.1 25.822 213 146 8 232 439 195 400 6.27e-11 64.7

Find 91 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTTAACCAACTCAGATTT+TGG 0.122678 2:-20578352 None:intergenic
CACAAAACTTGGTACCTTTC+AGG 0.146574 2:+20577334 MsaT007608.1:CDS
TTCAATTGTCCACAAAATTT+TGG 0.188550 2:+20578163 MsaT007608.1:CDS
ATGAAATTCTTTGAATCTTT+AGG 0.253896 2:+20577905 MsaT007608.1:CDS
GGATACACCGTGGAAGAAAT+TGG 0.270831 2:+20578238 MsaT007608.1:CDS
TTATCCTTAACAGGTGGATT+TGG 0.286270 2:-20577551 None:intergenic
AAAGAAAATGCAAGTTGATT+AGG 0.286687 2:+20578516 MsaT007608.1:CDS
TTCTCATTATCCTCACTAAA+AGG 0.287136 2:+20577837 MsaT007608.1:CDS
TTGTGTTACGGGCTTTATAT+TGG 0.339450 2:-20578200 None:intergenic
GAGAGAAGGTGATCAATAAA+AGG 0.354084 2:-20577295 None:intergenic
GGGATTATGATTATGAGATA+TGG 0.366062 2:+20578259 MsaT007608.1:CDS
CTATCCAAATCCACCTGTTA+AGG 0.367312 2:+20577547 MsaT007608.1:CDS
GCGTCCGATTTATCCTTAAC+AGG 0.379368 2:-20577560 None:intergenic
CTGAGAATTTGATGCCGAAA+TGG 0.384614 2:+20578305 MsaT007608.1:CDS
TCCACCTGTTAAGGATAAAT+CGG 0.398567 2:+20577556 MsaT007608.1:CDS
TAAAGATTCAAAGAATTTCA+TGG 0.398908 2:-20577903 None:intergenic
TTTACCCTCCAAACTATAGT+AGG 0.403290 2:-20577762 None:intergenic
ACACTGTAACTAACAATAGT+TGG 0.403606 2:-20578073 None:intergenic
GGTGAGATTACTGTTGAATC+AGG 0.408489 2:+20578444 MsaT007608.1:CDS
AGCTTACAACTCTTGAGATC+AGG 0.409547 2:+20577453 MsaT007608.1:CDS
CTAGGACTAGGAGGTGGAAT+TGG 0.414937 2:-20577584 None:intergenic
AAACTATAGTAGGCAAAAGC+TGG 0.426748 2:-20577752 None:intergenic
GTCGCATAATAGTCCTAATC+TGG 0.437513 2:-20577724 None:intergenic
GCAATGTACTTCCGTTCTCT+AGG 0.440973 2:+20578118 MsaT007608.1:CDS
AACCCTATTAGCAGCAGAAA+AGG 0.445757 2:+20577186 MsaT007608.1:CDS
CAATGTACTTCCGTTCTCTA+GGG 0.451044 2:+20578119 MsaT007608.1:CDS
TGCTATAAGTTAGCAGGGCT+GGG 0.457629 2:-20577967 None:intergenic
GTGCTATAAGTTAGCAGGGC+TGG 0.458589 2:-20577968 None:intergenic
GTGGACAATTGAATAGAAGA+AGG 0.464746 2:-20578153 None:intergenic
TCCTCATATTGCTTATCTGA+TGG 0.465202 2:+20577682 MsaT007608.1:CDS
TGAGAATTTGATGCCGAAAT+GGG 0.466974 2:+20578306 MsaT007608.1:CDS
CTCAACTTCCTTCCTCTACA+AGG 0.472025 2:+20576991 MsaT007608.1:CDS
GAATGAATATTGACCAGATT+AGG 0.481227 2:+20577711 MsaT007608.1:CDS
GGACGAAGATTTCCCTCAGC+AGG 0.482343 2:-20577662 None:intergenic
TCTGCGTGCTATAAGTTAGC+AGG 0.485788 2:-20577973 None:intergenic
CTTCTGAGACACCAAATTCA+CGG 0.490228 2:-20578021 None:intergenic
GATACACCGTGGAAGAAATT+GGG 0.490444 2:+20578239 MsaT007608.1:CDS
AGGTGTTGACAAGGAACAAT+GGG 0.491795 2:+20577925 MsaT007608.1:CDS
TCTGCTGCTAATAGGGTTGG+AGG 0.494961 2:-20577181 None:intergenic
ACTAAAAGGCCTCAATTATG+TGG 0.498259 2:+20577851 MsaT007608.1:CDS
CACTGTAACTAACAATAGTT+GGG 0.500646 2:-20578072 None:intergenic
GGATTACCCAAAATCTGAGT+TGG 0.502609 2:+20578345 MsaT007608.1:CDS
TAGAGTGAAGCTTCGAGAGA+AGG 0.507356 2:-20577309 None:intergenic
CATAATCCCAATTTCTTCCA+CGG 0.513081 2:-20578245 None:intergenic
AAGTTAGCAGGGCTGGGAAA+CGG 0.513243 2:-20577961 None:intergenic
CGTTCTCTAGGGGTTGATGT+TGG 0.515834 2:+20578130 MsaT007608.1:CDS
AAACAAGGTTAATACAGCCT+TGG 0.517039 2:-20577215 None:intergenic
TTCACTCTAAACACAAAACT+TGG 0.527791 2:+20577323 MsaT007608.1:CDS
ATTGTGGGGTGGTTGAATTG+AGG 0.533017 2:-20576342 None:intergenic
ATGGCACGGAATGGAAATTG+TGG 0.536305 2:-20576358 None:intergenic
AACTATAGTAGGCAAAAGCT+GGG 0.542556 2:-20577751 None:intergenic
TTTGCCTACTATAGTTTGGA+GGG 0.546380 2:+20577758 MsaT007608.1:CDS
AAGAAGACCCTTGTAGAGGA+AGG 0.547271 2:-20576999 None:intergenic
TCCATCAGATAAGCAATATG+AGG 0.550329 2:-20577683 None:intergenic
TTAGAGTCTACCGCTGGACT+AGG 0.566955 2:-20577602 None:intergenic
AGCAGAAAAGGAAGAAGCCA+AGG 0.567349 2:+20577198 MsaT007608.1:CDS
GCTTACAACTCTTGAGATCA+GGG 0.568117 2:+20577454 MsaT007608.1:CDS
TCCGATTTATCCTTAACAGG+TGG 0.568259 2:-20577557 None:intergenic
TGGCACGGAATGGAAATTGT+GGG 0.570971 2:-20576357 None:intergenic
GATTCAAAGAATTTCATGGT+GGG 0.572446 2:-20577899 None:intergenic
AGAAATTCTAGTGGATGTAG+TGG 0.577348 2:+20577520 MsaT007608.1:CDS
AATGTACTTCCGTTCTCTAG+GGG 0.585781 2:+20578120 MsaT007608.1:CDS
ACTTTCTTAGAGTCTACCGC+TGG 0.586538 2:-20577608 None:intergenic
GGAGGGTAAAATTAAGCCAG+TGG 0.594688 2:+20577775 MsaT007608.1:CDS
AGAAAAGAAGACCCTTGTAG+AGG 0.600416 2:-20577003 None:intergenic
ATTGCTTATCTGATGGAACT+CGG 0.604771 2:+20577689 MsaT007608.1:CDS
TGAATCTTTAGGTGTTGACA+AGG 0.607852 2:+20577916 MsaT007608.1:CDS
AGACTGATTCCACATAATTG+AGG 0.610049 2:-20577860 None:intergenic
TAGGTGTTGACAAGGAACAA+TGG 0.611520 2:+20577924 MsaT007608.1:CDS
TATTGTTAGTTACAGTGTAG+AGG 0.611669 2:+20578078 MsaT007608.1:CDS
TTGTAGCAACCAAACCTGAA+AGG 0.613581 2:-20577348 None:intergenic
CAACATCAACCCCTAGAGAA+CGG 0.618334 2:-20578129 None:intergenic
CTGCGTGCTATAAGTTAGCA+GGG 0.619373 2:-20577972 None:intergenic
GAATCACTTTACGAAGAGCA+TGG 0.623459 2:+20577497 MsaT007608.1:CDS
TATACCACTCACTCGAGACA+CGG 0.626260 2:-20577636 None:intergenic
ACCTCCTAGTCCTAGTCCAG+CGG 0.630206 2:+20577592 MsaT007608.1:CDS
GACGAAGATTTCCCTCAGCA+GGG 0.635800 2:-20577661 None:intergenic
TCTACCGCTGGACTAGGACT+AGG 0.636230 2:-20577596 None:intergenic
GGTGTCTCAGAAGAGAACAT+AGG 0.641929 2:+20578031 MsaT007608.1:CDS
AAAGCCGTGTCTCGAGTGAG+TGG 0.646198 2:+20577632 MsaT007608.1:CDS
AGAGCATGGAGAAATTCTAG+TGG 0.648996 2:+20577511 MsaT007608.1:CDS
GCTGGACTAGGACTAGGAGG+TGG 0.650924 2:-20577590 None:intergenic
GAGTGGTATAGACCCTGCTG+AGG 0.656302 2:+20577649 MsaT007608.1:CDS
GAACGGAAGTACATTGCTGT+CGG 0.657257 2:-20578112 None:intergenic
TCAACTTCCTTCCTCTACAA+GGG 0.663747 2:+20576992 MsaT007608.1:CDS
AGTGGTATAGACCCTGCTGA+GGG 0.666006 2:+20577650 MsaT007608.1:CDS
ACCGCTGGACTAGGACTAGG+AGG 0.679156 2:-20577593 None:intergenic
AGATTCAAAGAATTTCATGG+TGG 0.685200 2:-20577900 None:intergenic
ACGGAATGGAAATTGTGGGG+TGG 0.687703 2:-20576353 None:intergenic
GGCACGGAATGGAAATTGTG+GGG 0.705646 2:-20576356 None:intergenic
CTTGGAGAGAGGATACACCG+TGG 0.810527 2:+20578228 MsaT007608.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 20576329 20578537 20576329 ID=MsaG007608
Chr2 mRNA 20576329 20578537 20576329 ID=MsaT007608.1;Parent=MsaG007608
Chr2 exon 20576329 20576402 20576329 ID=MsaT007608.1.exon1;Parent=MsaT007608.1
Chr2 CDS 20576329 20576402 20576329 ID=cds.MsaT007608.1;Parent=MsaT007608.1
Chr2 exon 20576977 20577038 20576977 ID=MsaT007608.1.exon2;Parent=MsaT007608.1
Chr2 CDS 20576977 20577038 20576977 ID=cds.MsaT007608.1;Parent=MsaT007608.1
Chr2 exon 20577149 20577355 20577149 ID=MsaT007608.1.exon3;Parent=MsaT007608.1
Chr2 CDS 20577149 20577355 20577149 ID=cds.MsaT007608.1;Parent=MsaT007608.1
Chr2 exon 20577447 20578537 20577447 ID=MsaT007608.1.exon4;Parent=MsaT007608.1
Chr2 CDS 20577447 20578537 20577447 ID=cds.MsaT007608.1;Parent=MsaT007608.1
Gene Sequence

>MsaT007608.1

ATGAAAACTTTCTCCTCAATTCAACCACCCCACAATTTCCATTCCGTGCCATTTTTCTCTTACACTTCCATAAGATTTTGCATCAGGACTCAACTTCCTTCCTCTACAAGGGTCTTCTTTTCTCAGGAAAAATCTGACACAGATGAGTCATTAAACGTAAAACTGTTGCCTCCAACCCTATTAGCAGCAGAAAAGGAAGAAGCCAAGGCTGTATTAACCTTGTTTTTGAAGAAACGAGGTTTGAGCAATGCAAATGCAGCAAGAACGATTAACAAGTCAGATCCTTTTATTGATCACCTTCTCTCGAAGCTTCACTCTAAACACAAAACTTGGTACCTTTCAGGGAGAGAGCTTACAACTCTTGAGATCAGGGATGCTCTTATCTCTTATCTCGAATCACTTTACGAAGAGCATGGAGAAATTCTAGTGGATGTAGTGGAAAACTATCCAAATCCACCTGTTAAGGATAAATCGGACGCACCAATTCCACCTCCTAGTCCTAGTCCAGCGGTAGACTCTAAGAAAGTTAAAGCCGTGTCTCGAGTGAGTGGTATAGACCCTGCTGAGGGAAATCTTCGTCCTCATATTGCTTATCTGATGGAACTCGGAATGAATATTGACCAGATTAGGACTATTATGCGACGATTCCCAGCTTTTGCCTACTATAGTTTGGAGGGTAAAATTAAGCCAGTGGTTGAGTTTTTTCTTGAACTTGGAGTACCAAAAGAAAAAATTCTCATTATCCTCACTAAAAGGCCTCAATTATGTGGAATCAGTCTATCTAAAAATCTTAAGCCCACCATGAAATTCTTTGAATCTTTAGGTGTTGACAAGGAACAATGGGCGAAAGTGATCTACCGTTTCCCAGCCCTGCTAACTTATAGCACGCAGAAGATTAATGAAAGTGTAGATTTTCTCCGTGAATTTGGTGTCTCAGAAGAGAACATAGGTAAGATTTTAACTCGCTGCCCAACTATTGTTAGTTACAGTGTAGAGGACAATCTTCGACCGACAGCAATGTACTTCCGTTCTCTAGGGGTTGATGTTGGCCTTCTTCTATTCAATTGTCCACAAAATTTTGGTCTTAGCATTGAGGCCAATATAAAGCCCGTAACACAATTTTTCTTGGAGAGAGGATACACCGTGGAAGAAATTGGGATTATGATTATGAGATATGGAATGTTGTACACATTCAGCTTGACTGAGAATTTGATGCCGAAATGGGATTACTTTTTGACTATGGATTACCCAAAATCTGAGTTGGTTAAATTCCCTCAATTTTTTGGATACAGTTTAGAGCAAAGAATTAAACCGAGATATGCACGCGTGAAAAATTCTGGGGTGAGATTACTGTTGAATCAGGTTCTTTCACTGTCAAGCAGCAACTTTGACGAGATCTTAAGAAAGAAAATAAAGAAAATGCAAGTTGATTAG
Protein sequence

>MsaT007608.1

MKTFSSIQPPHNFHSVPFFSYTSIRFCIRTQLPSSTRVFFSQEKSDTDESLNVKLLPPTLLAAEKEEAKAVLTLFLKKRGLSNANAARTINKSDPFIDHLLSKLHSKHKTWYLSGRELTTLEIRDALISYLESLYEEHGEILVDVVENYPNPPVKDKSDAPIPPPSPSPAVDSKKVKAVSRVSGIDPAEGNLRPHIAYLMELGMNIDQIRTIMRRFPAFAYYSLEGKIKPVVEFFLELGVPKEKILIILTKRPQLCGISLSKNLKPTMKFFESLGVDKEQWAKVIYRFPALLTYSTQKINESVDFLREFGVSEENIGKILTRCPTIVSYSVEDNLRPTAMYFRSLGVDVGLLLFNCPQNFGLSIEANIKPVTQFFLERGYTVEEIGIMIMRYGMLYTFSLTENLMPKWDYFLTMDYPKSELVKFPQFFGYSLEQRIKPRYARVKNSGVRLLLNQVLSLSSSNFDEILRKKIKKMQVD*