AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007628

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007628 MtrunA17_Chr2g0294841 98.387 682 11 0 66 747 80 761 0.0 1425
MsaG007628 MtrunA17_Chr2g0276841 59.899 596 234 3 66 661 105 695 0.0 764
MsaG007628 MtrunA17_Chr1g0198761 42.965 661 356 6 72 731 93 733 0.0 558
MsaG007628 MtrunA17_Chr2g0290391 40.956 586 327 3 72 647 90 666 2.59e-155 467
MsaG007628 MtrunA17_Chr5g0431001 49.091 385 193 1 72 456 107 488 1.02e-131 400
MsaG007628 MtrunA17_Chr5g0448761 27.349 596 412 11 72 652 100 689 1.83e-73 254
MsaG007628 MtrunA17_Chr1g0202341 27.664 535 366 10 62 589 84 604 4.82e-62 221
MsaG007628 MtrunA17_Chr2g0329031 27.828 557 357 14 56 597 38 564 5.13e-60 216
MsaG007628 MtrunA17_Chr5g0441581 28.184 479 313 9 127 590 117 579 7.32e-59 212
MsaG007628 MtrunA17_Chr3g0080351 27.969 522 338 10 74 589 129 618 8.36e-58 211
MsaG007628 MtrunA17_Chr8g0363841 26.036 603 396 14 76 648 62 644 3.92e-54 198
MsaG007628 MtrunA17_Chr3g0118611 30.100 402 263 7 205 596 110 503 5.84e-54 199
MsaG007628 MtrunA17_Chr2g0287651 26.981 530 357 9 73 589 115 627 9.41e-54 199
MsaG007628 MtrunA17_Chr3g0082121 28.067 538 363 10 65 599 79 595 7.08e-53 195
MsaG007628 MtrunA17_Chr1g0207551 27.938 519 353 9 78 589 80 584 2.78e-52 194
MsaG007628 MtrunA17_Chr5g0448511 29.759 373 256 4 222 589 21 392 6.01e-52 189
MsaG007628 MtrunA17_Chr2g0296781 28.631 482 298 13 136 590 90 552 1.54e-51 191
MsaG007628 MtrunA17_Chr5g0440451 25.746 536 353 8 76 589 87 599 8.65e-50 188
MsaG007628 MtrunA17_Chr6g0477261 27.540 443 306 9 160 590 2 441 1.30e-47 179
MsaG007628 MtrunA17_Chr2g0324011 26.220 492 346 9 129 609 99 584 2.35e-46 174
MsaG007628 MtrunA17_Chr1g0203051 23.741 556 378 11 48 581 90 621 1.85e-44 169
MsaG007628 MtrunA17_Chr6g0458391 27.390 544 340 17 65 589 253 760 1.91e-44 171
MsaG007628 MtrunA17_Chr2g0287061 25.954 524 330 11 73 589 224 696 1.20e-43 169
MsaG007628 MtrunA17_Chr1g0203721 24.771 545 373 12 65 589 91 618 8.82e-42 164
MsaG007628 MtrunA17_Chr1g0196541 23.956 551 368 12 136 666 90 609 4.67e-41 160
MsaG007628 MtrunA17_Chr2g0291581 24.048 499 351 7 103 589 134 616 8.89e-34 138
MsaG007628 MtrunA17_Chr1g0164591 26.415 318 223 3 116 432 1 308 5.09e-33 130
MsaG007628 MtrunA17_Chr3g0081281 54.839 93 42 0 177 269 1 93 8.54e-30 113
MsaG007628 MtrunA17_Chr2g0291591 24.786 468 308 12 98 543 122 567 1.59e-29 124
MsaG007628 MtrunA17_Chr7g0258861 26.070 257 177 6 62 315 42 288 7.90e-16 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007628 AT1G10240.2 58.361 598 244 3 66 663 83 675 0.0 749
MsaG007628 AT1G10240.1 58.361 598 244 3 66 663 83 675 0.0 749
MsaG007628 AT5G28530.3 40.388 619 328 8 56 648 67 670 3.89e-151 456
MsaG007628 AT5G28530.2 40.388 619 328 8 56 648 67 670 3.89e-151 456
MsaG007628 AT5G28530.1 39.903 619 335 7 56 648 67 674 1.17e-150 455
MsaG007628 AT5G28530.5 36.782 609 301 9 56 648 67 607 9.35e-117 365
MsaG007628 AT5G28530.4 36.289 609 308 8 56 648 67 611 2.53e-116 364
MsaG007628 AT4G38180.1 29.944 531 359 8 72 596 113 636 5.80e-76 261
MsaG007628 AT4G15090.1 26.394 538 381 9 107 636 122 652 1.54e-57 210
MsaG007628 AT4G15090.5 26.394 538 381 9 107 636 122 652 1.54e-57 210
MsaG007628 AT4G15090.3 26.394 538 381 9 107 636 122 652 1.54e-57 210
MsaG007628 AT4G15090.2 26.394 538 381 9 107 636 122 652 1.54e-57 210
MsaG007628 AT4G15090.4 26.394 538 381 9 107 636 122 652 1.54e-57 210
MsaG007628 AT3G22170.2 25.704 568 370 15 65 614 103 636 2.33e-53 198
MsaG007628 AT3G22170.1 25.704 568 370 15 65 614 103 636 2.33e-53 198
MsaG007628 AT1G76320.3 26.401 553 372 9 47 589 12 539 2.35e-52 193
MsaG007628 AT1G76320.2 26.401 553 372 9 47 589 12 539 8.99e-52 192
MsaG007628 AT1G76320.1 26.582 553 371 9 47 589 12 539 9.46e-52 192
MsaG007628 AT1G76320.4 26.582 553 371 9 47 589 12 539 9.46e-52 192
MsaG007628 AT2G27110.2 27.607 489 323 12 110 589 108 574 1.40e-48 184
MsaG007628 AT2G27110.1 27.607 489 323 12 110 589 108 574 1.40e-48 184
MsaG007628 AT2G27110.3 30.026 383 258 7 213 589 51 429 1.03e-47 181
MsaG007628 AT4G19990.5 24.825 572 370 10 36 589 25 554 4.89e-43 167
MsaG007628 AT4G19990.3 24.825 572 370 10 36 589 25 554 6.26e-43 167
MsaG007628 AT4G19990.4 24.825 572 370 10 36 589 25 554 6.26e-43 167
MsaG007628 AT4G19990.7 24.825 572 370 10 36 589 39 568 6.87e-43 167
MsaG007628 AT4G19990.6 24.825 572 370 10 36 589 25 554 8.92e-43 166
MsaG007628 AT4G19990.2 24.825 572 370 10 36 589 25 554 8.92e-43 166
MsaG007628 AT4G38170.1 28.457 376 254 7 222 589 26 394 2.61e-42 162
MsaG007628 AT4G19990.1 25.660 530 334 10 78 589 10 497 2.70e-42 164
MsaG007628 AT3G06250.2 27.052 536 305 17 73 589 231 699 5.41e-42 164
MsaG007628 AT3G06250.1 27.052 536 305 17 73 589 231 699 5.41e-42 164
MsaG007628 AT3G06250.3 27.052 536 305 17 73 589 231 699 5.41e-42 164
MsaG007628 AT1G52520.1 26.418 564 359 16 99 634 144 679 2.71e-41 161
MsaG007628 AT5G18960.2 25.143 525 332 14 73 589 252 723 9.89e-39 154
MsaG007628 AT5G18960.1 25.143 525 332 14 73 589 252 723 9.89e-39 154
MsaG007628 AT1G80010.1 25.192 520 345 13 99 590 154 657 4.17e-37 149
MsaG007628 AT2G32250.8 22.378 572 367 11 37 593 2 511 1.04e-36 148
MsaG007628 AT2G32250.5 22.478 565 375 12 37 593 43 552 1.13e-36 148
MsaG007628 AT2G32250.7 22.378 572 367 11 37 593 2 511 1.21e-36 148
MsaG007628 AT2G32250.6 22.378 572 367 11 37 593 43 552 1.22e-36 148
MsaG007628 AT2G32250.1 22.378 572 367 11 37 593 43 552 1.22e-36 148
MsaG007628 AT2G32250.3 22.378 572 367 11 37 593 43 552 1.22e-36 148
MsaG007628 AT2G32250.2 22.378 572 367 11 37 593 43 552 1.58e-36 147
MsaG007628 AT2G32250.4 22.378 572 367 11 37 593 43 552 1.58e-36 147

Find 157 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCTTCAGAAGGTTTATTT+TGG 0.139353 2:-20851075 None:intergenic
TCCAAATATTGTATCCTTTC+AGG 0.144098 2:-20854532 None:intergenic
ATGACATGCTGTTGGGAATT+TGG 0.166402 2:+20851641 MsaT007628.1:CDS
ATTCAATCATATGAGGTATT+TGG 0.211487 2:+20851577 MsaT007628.1:CDS
GTTCCATCGGCTCTACAATT+TGG 0.214855 2:+20853726 MsaT007628.1:CDS
ACCTCAATATGCATCCTTTC+TGG 0.234586 2:+20854252 MsaT007628.1:CDS
GATACAATATTTGGAATCTT+TGG 0.243804 2:+20854540 MsaT007628.1:CDS
GCCTGAAAGGATACAATATT+TGG 0.244750 2:+20854531 MsaT007628.1:CDS
TATACTCATTAAGGACATTC+TGG 0.255249 2:+20853830 MsaT007628.1:CDS
TCACATTATGCAGGCTTTCT+TGG 0.257492 2:+20853522 MsaT007628.1:intron
TTTGGTTTCAACACTTCTAA+TGG 0.258812 2:+20854558 MsaT007628.1:CDS
CAAAAGGCATGTTTACTTTC+GGG 0.259449 2:-20853625 None:intergenic
CTTGACAGAATTGTTTCTTC+TGG 0.282713 2:-20850865 None:intergenic
GTCCCTGAATGGCGTGTTAC+TGG 0.284993 2:+20851187 MsaT007628.1:CDS
AAGGCAAATGTTAAGATTAA+TGG 0.285271 2:+20851336 MsaT007628.1:CDS
TGCTAGTTGCGCCATGAAAA+TGG 0.292848 2:-20854501 None:intergenic
TTGAGGCGATATTTCTTTGC+AGG 0.296991 2:+20853865 MsaT007628.1:CDS
ACAAATCTTTGACCAATAAA+AGG 0.311831 2:-20850910 None:intergenic
CACATTATGCAGGCTTTCTT+GGG 0.312370 2:+20853523 MsaT007628.1:intron
CCGTTGGATGAAAACGTTAA+TGG 0.319103 2:-20853914 None:intergenic
GTACATGCGTATTGTGAAAA+AGG 0.319270 2:+20851153 MsaT007628.1:CDS
TGGCTTGGATTGGACAATAA+TGG 0.319336 2:+20851661 MsaT007628.1:CDS
ACAAAAGGCATGTTTACTTT+CGG 0.321090 2:-20853626 None:intergenic
ATGGAGGTTGCAAAGTGTTT+TGG 0.321516 2:+20854317 MsaT007628.1:CDS
TTATTAGCCTATACTCATTA+AGG 0.323374 2:+20853821 MsaT007628.1:CDS
CCTCATGATTCCATGGCTTT+AGG 0.327738 2:+20854772 MsaT007628.1:CDS
CTCACATCTATCTCTGTAAA+AGG 0.340205 2:-20851391 None:intergenic
AGGCAAATGGTGGATAAATA+TGG 0.340475 2:+20853778 MsaT007628.1:CDS
AACCACAGCGAGATGATTTA+CGG 0.342181 2:-20851123 None:intergenic
AAACCAGTAACACGCCATTC+AGG 0.361653 2:-20851190 None:intergenic
TCACCATTATGTGCACAATA+TGG 0.372426 2:+20854859 MsaT007628.1:CDS
CTTTAGCAAACATGCATATA+CGG 0.380754 2:-20851300 None:intergenic
GATACTGATGAAATGATTCA+TGG 0.382834 2:+20854733 MsaT007628.1:CDS
TTCTCTCCAAGTTCTCTGAT+TGG 0.385450 2:+20853659 MsaT007628.1:CDS
CTTTAGTTGCGCCCTTCTGC+GGG 0.388321 2:+20851696 MsaT007628.1:CDS
GTGCTGCAACTAGCTCTTCC+TGG 0.388829 2:-20854231 None:intergenic
CTCTTATCATCAGGAGATAT+CGG 0.409413 2:-20851277 None:intergenic
CAAAATAAACCTTCTGAAGA+TGG 0.410862 2:+20851076 MsaT007628.1:CDS
GTTGGGAATTTGGCTTGGAT+TGG 0.415439 2:+20851651 MsaT007628.1:CDS
TCAAGATAGATAATAATAAC+AGG 0.416672 2:+20851521 MsaT007628.1:CDS
CAGAAAGTCTGCTTGAAAAC+AGG 0.419908 2:+20854151 MsaT007628.1:CDS
TGATCAGTCAGTATTGTTTG+TGG 0.423171 2:-20853565 None:intergenic
TGGATCTGATTGCTCAATAA+TGG 0.423232 2:+20854888 MsaT007628.1:CDS
AGATTAATGGAGCTAGAAAA+AGG 0.423796 2:+20851349 MsaT007628.1:CDS
TTGAATAAGACGCACCTCAT+TGG 0.427145 2:-20851245 None:intergenic
AATATGAGTGAAGATGCATT+AGG 0.432322 2:+20854919 MsaT007628.1:CDS
AATTCCCAACAGCATGTCAT+AGG 0.437999 2:-20851638 None:intergenic
TGGAGTTTGCTCAAGGCATA+AGG 0.440065 2:-20854211 None:intergenic
ATGCTGTTGGGAATTTGGCT+TGG 0.440367 2:+20851646 MsaT007628.1:CDS
ATATGCATGTTTGCTAAAGC+TGG 0.454030 2:+20851304 MsaT007628.1:CDS
GACACCGCACCGAGGAAAGA+TGG 0.454060 2:+20850998 MsaT007628.1:CDS
TCATCAGTATCTTCTACCTC+TGG 0.460431 2:-20854721 None:intergenic
AAGAAATATCGCCTCAAGAA+TGG 0.462464 2:-20853859 None:intergenic
CCGATCCGATGGCGAAGCAT+TGG 0.468854 2:+20854463 MsaT007628.1:CDS
CATATACGGCTCTTATCATC+AGG 0.473122 2:-20851286 None:intergenic
ATATGAGTGAAGATGCATTA+GGG 0.474188 2:+20854920 MsaT007628.1:CDS
GAGAATATGCCATATACAAA+AGG 0.474322 2:-20853641 None:intergenic
GCTTTAGTTGCGCCCTTCTG+CGG 0.475590 2:+20851695 MsaT007628.1:CDS
AAAATAAACCTTCTGAAGAT+GGG 0.478175 2:+20851077 MsaT007628.1:CDS
CGGATCGGTAAATATTGATC+CGG 0.479172 2:-20854448 None:intergenic
CTCATCAATTCAATCATATG+AGG 0.479395 2:+20851570 MsaT007628.1:CDS
CGGCCAACCCCATCTTTCCT+CGG 0.480137 2:-20851007 None:intergenic
TGTCCATATTGTGCACATAA+TGG 0.485930 2:-20854862 None:intergenic
GTGGCAGCATGGGATTCTAT+AGG 0.487656 2:-20854178 None:intergenic
ATAAAACCAATCAGAGAACT+TGG 0.489580 2:-20853665 None:intergenic
CTTGGGCTTCATGAAAGGAA+AGG 0.491097 2:+20853540 MsaT007628.1:CDS
ATACTCATTAAGGACATTCT+GGG 0.491639 2:+20853831 MsaT007628.1:CDS
AGGAAAGATGGGGTTGGCCG+AGG 0.499837 2:+20851010 MsaT007628.1:CDS
TTATGCCTTGAGCAAACTCC+AGG 0.504552 2:+20854213 MsaT007628.1:CDS
TGCTTGTGACCAAGTTTCAA+TGG 0.504988 2:+20854612 MsaT007628.1:CDS
ACACCGCACCGAGGAAAGAT+GGG 0.507773 2:+20850999 MsaT007628.1:CDS
GCTTTCTTGGGCTTCATGAA+AGG 0.511793 2:+20853535 MsaT007628.1:CDS
GAGGCCTATGACATGCTGTT+GGG 0.513583 2:+20851634 MsaT007628.1:CDS
CATGGCTTTAGGCAAGCTAA+AGG 0.513686 2:+20854783 MsaT007628.1:CDS
TGAAGAAAGCTGGAGGCAAA+TGG 0.514494 2:+20853765 MsaT007628.1:CDS
AAGGCGTTCCTCGGTTTCAA+CGG 0.516469 2:-20854584 None:intergenic
CTCTTCCTGGAGTTTGCTCA+AGG 0.516928 2:-20854218 None:intergenic
AACCAGTAACACGCCATTCA+GGG 0.519550 2:-20851189 None:intergenic
TGCAAATCCTCATGATTCCA+TGG 0.519849 2:+20854765 MsaT007628.1:CDS
CTTTAGCTTGCCTAAAGCCA+TGG 0.519923 2:-20854782 None:intergenic
AGAATGTCCTTAATGAGTAT+AGG 0.523345 2:-20853828 None:intergenic
GAGTACATTGCATGGTCCTA+CGG 0.523416 2:+20851547 MsaT007628.1:CDS
AGCAACATCAAGGCGTTCCT+CGG 0.524644 2:-20854593 None:intergenic
AAGGAGAGAAGGGCAAGAGA+TGG 0.526646 2:+20854802 MsaT007628.1:CDS
GCATCCTTTCTGGTTGATGA+AGG 0.529559 2:+20854262 MsaT007628.1:CDS
CTAGCAGGGATCAGCCTGAA+AGG 0.537919 2:+20854518 MsaT007628.1:CDS
AAATGGCACAGATTTGTCTC+AGG 0.538047 2:+20850816 None:intergenic
ATTTACGCCATGTGATGACC+TGG 0.539389 2:-20854659 None:intergenic
AACCTCCATCGGTCTGAGAA+TGG 0.539487 2:-20854303 None:intergenic
AAACACTTTGCAACCTCCAT+CGG 0.539933 2:-20854314 None:intergenic
GGACTCGGTCTGACATGTAC+TGG 0.541636 2:-20853893 None:intergenic
GGCATAAGGAGTAAGTATAG+TGG 0.542397 2:-20854197 None:intergenic
AGAGGCCTATGACATGCTGT+TGG 0.543489 2:+20851633 MsaT007628.1:CDS
CAGTATCTTCTACCTCTGGC+AGG 0.547423 2:-20854717 None:intergenic
TAGGCAAGCTAAAGGAGAGA+AGG 0.550540 2:+20854791 MsaT007628.1:CDS
TCTTGCATTTAGTTATGCAT+CGG 0.553812 2:+20854684 MsaT007628.1:CDS
ATCAATATTTACCGATCCGA+TGG 0.556315 2:+20854452 MsaT007628.1:CDS
ACCAGAAAGGATGCATATTG+AGG 0.558654 2:-20854253 None:intergenic
AGTAAGTATAGTGGCAGCAT+GGG 0.558789 2:-20854188 None:intergenic
GCATACTTTCATCCCGCAGA+AGG 0.560022 2:-20851708 None:intergenic
CTAAAGCTGGAATGTCGGTA+AGG 0.564431 2:+20851317 MsaT007628.1:CDS
GAGTAAGTATAGTGGCAGCA+TGG 0.565564 2:-20854189 None:intergenic
GTGATAGCACCATTGAAACT+TGG 0.565620 2:-20854621 None:intergenic
AGTGGAAAGCTGAGTTCCAT+CGG 0.569331 2:+20853713 MsaT007628.1:CDS
CTTGGTCACAAGCAACATCA+AGG 0.570174 2:-20854603 None:intergenic
TGTGCACAATATGGACATGA+TGG 0.571985 2:+20854868 MsaT007628.1:CDS
CCAATGCTTCGCCATCGGAT+CGG 0.572305 2:-20854463 None:intergenic
TGAGGTATTTGGTGATGCTG+TGG 0.573633 2:+20851588 MsaT007628.1:CDS
AGGCAAGCTAAAGGAGAGAA+GGG 0.573940 2:+20854792 MsaT007628.1:CDS
ACAACCTTCATCAACCAGAA+AGG 0.583485 2:-20854266 None:intergenic
TTGGGTCGCAATACGATGAG+TGG 0.584461 2:+20853695 MsaT007628.1:CDS
TCTGGGCTTTACCATTCTTG+AGG 0.584691 2:+20853848 MsaT007628.1:CDS
TTTCCAAATTGTAGAGCCGA+TGG 0.585557 2:-20853729 None:intergenic
GAGCAAAGCAAAAGATGCAA+AGG 0.586020 2:+20854122 MsaT007628.1:CDS
CAGCTGATGTGCTCTTGACA+AGG 0.588190 2:-20854343 None:intergenic
AATCCCTCCTCGTTACTCCT+CGG 0.588500 2:-20851027 None:intergenic
ACAGACCAATGCTTCGCCAT+CGG 0.588693 2:-20854468 None:intergenic
TCGGCTCTACAATTTGGAAA+CGG 0.592465 2:+20853732 MsaT007628.1:CDS
CATACTTTCATCCCGCAGAA+GGG 0.593580 2:-20851707 None:intergenic
AAATAAACCTTCTGAAGATG+GGG 0.596801 2:+20851078 MsaT007628.1:CDS
GTCATAGGCCTCTACTCGGT+AGG 0.599446 2:-20851623 None:intergenic
AACCCCATCTTTCCTCGGTG+CGG 0.602286 2:-20851002 None:intergenic
CACGTGTTAAAACTCTTCCC+AGG 0.602561 2:+20854641 MsaT007628.1:CDS
GCATGTCATAGGCCTCTACT+CGG 0.603797 2:-20851627 None:intergenic
ATTGTGCACTCAAGAACCGT+TGG 0.604864 2:-20853930 None:intergenic
TGATACAACCTACCGAGTAG+AGG 0.608589 2:+20851615 MsaT007628.1:CDS
GGGGAGGAGGAAAAGCGCAA+AGG 0.609723 2:+20851098 MsaT007628.1:CDS
GTTTGCTAAAGCTGGAATGT+CGG 0.610570 2:+20851312 MsaT007628.1:CDS
GCACCGAGGAAAGATGGGGT+TGG 0.613357 2:+20851004 MsaT007628.1:CDS
TGCCTGAAAACTCAAATAGA+CGG 0.614950 2:-20854372 None:intergenic
GGATCGGTAAATATTGATCC+GGG 0.615359 2:-20854447 None:intergenic
CACCATTCTCAGACCGATGG+AGG 0.619807 2:+20854301 MsaT007628.1:CDS
GGATGACATTGGCACTGTCG+AGG 0.620402 2:+20850837 MsaT007628.1:CDS
TGATTGAATTGATGAGCCGT+AGG 0.623124 2:-20851563 None:intergenic
TGGCCGAGGAGTAACGAGGA+GGG 0.628175 2:+20851024 MsaT007628.1:CDS
AATGGAATCCGTTGAAACCG+AGG 0.632410 2:+20854576 MsaT007628.1:CDS
GATGAAAACGTTAATGGACT+CGG 0.633126 2:-20853908 None:intergenic
AGACACCATTCTCAGACCGA+TGG 0.638459 2:+20854298 MsaT007628.1:CDS
TTGGCCGAGGAGTAACGAGG+AGG 0.640151 2:+20851023 MsaT007628.1:CDS
CCTAAAGCCATGGAATCATG+AGG 0.642544 2:-20854772 None:intergenic
GATTTGTCTCAGGATGACAT+TGG 0.649568 2:+20850826 MsaT007628.1:CDS
AGAAAGCTGGAGGCAAATGG+TGG 0.650737 2:+20853768 MsaT007628.1:CDS
ACAGGCTCGAGTACATTGCA+TGG 0.652102 2:+20851539 MsaT007628.1:CDS
TACTGACTGATCATAACACA+TGG 0.653234 2:+20853575 MsaT007628.1:CDS
GCTCTACAATTTGGAAACGG+TGG 0.653467 2:+20853735 MsaT007628.1:CDS
TGAGTTGTTAAAATCCAATG+AGG 0.655034 2:+20851231 MsaT007628.1:CDS
TTTGACCAATAAAAGGAACA+AGG 0.658851 2:-20850903 None:intergenic
TTGATATTAGTAGAAAACGT+AGG 0.664945 2:+20854827 MsaT007628.1:CDS
ACTCTTCCCAGGTCATCACA+TGG 0.672595 2:+20854652 MsaT007628.1:CDS
CACCGCACCGAGGAAAGATG+GGG 0.676063 2:+20851000 MsaT007628.1:CDS
TTTACGCCATGTGATGACCT+GGG 0.676760 2:-20854658 None:intergenic
AATAAACCTTCTGAAGATGG+GGG 0.678235 2:+20851079 MsaT007628.1:CDS
TTTGGGGAAATCCTCCGCGG+TGG 0.687568 2:-20851057 None:intergenic
AACCGTAAATCATCTCGCTG+TGG 0.688131 2:+20851121 MsaT007628.1:CDS
GGATTCATTGATCCTGCCAG+AGG 0.692463 2:+20854705 MsaT007628.1:CDS
GGGTTGGCCGAGGAGTAACG+AGG 0.708315 2:+20851020 MsaT007628.1:CDS
AATTAGACGACACCGCACCG+AGG 0.751379 2:+20850990 MsaT007628.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 20850818 20854951 20850818 ID=MsaG007628
Chr2 mRNA 20850818 20854951 20850818 ID=MsaT007628.1;Parent=MsaG007628
Chr2 exon 20850818 20851753 20850818 ID=MsaT007628.1.exon1;Parent=MsaT007628.1
Chr2 CDS 20850818 20851753 20850818 ID=cds.MsaT007628.1;Parent=MsaT007628.1
Chr2 exon 20853535 20853978 20853535 ID=MsaT007628.1.exon2;Parent=MsaT007628.1
Chr2 CDS 20853535 20853978 20853535 ID=cds.MsaT007628.1;Parent=MsaT007628.1
Chr2 exon 20854088 20854951 20854088 ID=MsaT007628.1.exon3;Parent=MsaT007628.1
Chr2 CDS 20854088 20854951 20854088 ID=cds.MsaT007628.1;Parent=MsaT007628.1
Gene Sequence

>MsaT007628.1

ATGGCACAGATTTGTCTCAGGATGACATTGGCACTGTCGAGGAAACACCAGAAGAAACAATTCTGTCAAGACAAACTTCTGTTAACCTTGTTCCTTTTATTGGTCAAAGATTTGTCTCTCAAGAAGCTGCTTATGAATTTTATTGCAGTTTTGCTAAACAATTTGGCTTTTCAATTAGACGACACCGCACCGAGGAAAGATGGGGTTGGCCGAGGAGTAACGAGGAGGGATTTTACTTGCCACCGCGGAGGATTTCCCCAAAATAAACCTTCTGAAGATGGGGGAGGAGGAAAAGCGCAAAGGAACCGTAAATCATCTCGCTGTGGTTGTCAAGCGTACATGCGTATTGTGAAAAAGGCAGATTTTGATGTCCCTGAATGGCGTGTTACTGGTTTTAGAAACATCCACAATCATGAGTTGTTAAAATCCAATGAGGTGCGTCTTATTCAATCACACTGTCCGATATCTCCTGATGATAAGAGCCGTATATGCATGTTTGCTAAAGCTGGAATGTCGGTAAGGCAAATGTTAAGATTAATGGAGCTAGAAAAAGGCATCAAACTTGGTTGTCTTCCTTTTACAGAGATAGATGTGAGAAACTTGTTGCAGTCTTTTAGAAACGTGGATAAAGATAATGATGCTATTGATCTGATTGCAATGTGTAAGAGATTAAAAGATGAAAATCACAACTTCAAGTATGAATTCAAGATAGATAATAATAACAGGCTCGAGTACATTGCATGGTCCTACGGCTCATCAATTCAATCATATGAGGTATTTGGTGATGCTGTGGTTTTTGATACAACCTACCGAGTAGAGGCCTATGACATGCTGTTGGGAATTTGGCTTGGATTGGACAATAATGGAGTGATATGTTGCTTTAGTTGCGCCCTTCTGCGGGATGAAAGTATGCCGTCTTTTTCATGGGCTTTAAAGGCTTTCTTGGGCTTCATGAAAGGAAAGGCTCCACAAACAATACTGACTGATCATAACACATGGCTGAAAGAAGCTATTGCTGTTGAAATGCCCGAAAGTAAACATGCCTTTTGTATATGGCATATTCTCTCCAAGTTCTCTGATTGGTTTTATTTACTTCTTGGGTCGCAATACGATGAGTGGAAAGCTGAGTTCCATCGGCTCTACAATTTGGAAACGGTGGAAGATTTTGAAGAAAGCTGGAGGCAAATGGTGGATAAATATGGACTTCATGCAAATAAACATATTATTAGCCTATACTCATTAAGGACATTCTGGGCTTTACCATTCTTGAGGCGATATTTCTTTGCAGGATTAACCAGTACATGTCAGACCGAGTCCATTAACGTTTTCATCCAACGGTTCTTGAGTGCACAATCTCAACCAGAACGTTTTTTAGAGCAAGTGGCTGATATTGTTGATTTTAATGACCGAGCCGGAGCAAAGCAAAAGATGCAAAGGAAAATGCAGAAAGTCTGCTTGAAAACAGGTTCACCTATAGAATCCCATGCTGCCACTATACTTACTCCTTATGCCTTGAGCAAACTCCAGGAAGAGCTAGTTGCAGCACCTCAATATGCATCCTTTCTGGTTGATGAAGGTTGTTTTCAGGTTAGACACCATTCTCAGACCGATGGAGGTTGCAAAGTGTTTTGGCTTCCTTGTCAAGAGCACATCAGCTGCAGCTGCCGTCTATTTGAGTTTTCAGGCATCTTATGTAAACATGTGCTACGCGTCATGTCAACTAATAACTGTTTTCACATCCCGGATCAATATTTACCGATCCGATGGCGAAGCATTGGTCTGTCTTCTTTTAACCATTTTCATGGCGCAACTAGCAGGGATCAGCCTGAAAGGATACAATATTTGGAATCTTTGGTTTCAACACTTCTAATGGAATCCGTTGAAACCGAGGAACGCCTTGATGTTGCTTGTGACCAAGTTTCAATGGTGCTATCACGTGTTAAAACTCTTCCCAGGTCATCACATGGCGTAAATGATCTTGCATTTAGTTATGCATCGGATTCATTGATCCTGCCAGAGGTAGAAGATACTGATGAAATGATTCATGGTTTTACAATTGCAAATCCTCATGATTCCATGGCTTTAGGCAAGCTAAAGGAGAGAAGGGCAAGAGATGGAGTTGATATTAGTAGAAAACGTAGGCAGTTCTCATCACCATTATGTGCACAATATGGACATGATGGATCTGATTGCTCAATAATGGCTGATGATAATATGAGTGAAGATGCATTAGGGTATATGTAG
Protein sequence

>MsaT007628.1

MAQICLRMTLALSRKHQKKQFCQDKLLLTLFLLLVKDLSLKKLLMNFIAVLLNNLAFQLDDTAPRKDGVGRGVTRRDFTCHRGGFPQNKPSEDGGGGKAQRNRKSSRCGCQAYMRIVKKADFDVPEWRVTGFRNIHNHELLKSNEVRLIQSHCPISPDDKSRICMFAKAGMSVRQMLRLMELEKGIKLGCLPFTEIDVRNLLQSFRNVDKDNDAIDLIAMCKRLKDENHNFKYEFKIDNNNRLEYIAWSYGSSIQSYEVFGDAVVFDTTYRVEAYDMLLGIWLGLDNNGVICCFSCALLRDESMPSFSWALKAFLGFMKGKAPQTILTDHNTWLKEAIAVEMPESKHAFCIWHILSKFSDWFYLLLGSQYDEWKAEFHRLYNLETVEDFEESWRQMVDKYGLHANKHIISLYSLRTFWALPFLRRYFFAGLTSTCQTESINVFIQRFLSAQSQPERFLEQVADIVDFNDRAGAKQKMQRKMQKVCLKTGSPIESHAATILTPYALSKLQEELVAAPQYASFLVDEGCFQVRHHSQTDGGCKVFWLPCQEHISCSCRLFEFSGILCKHVLRVMSTNNCFHIPDQYLPIRWRSIGLSSFNHFHGATSRDQPERIQYLESLVSTLLMESVETEERLDVACDQVSMVLSRVKTLPRSSHGVNDLAFSYASDSLILPEVEDTDEMIHGFTIANPHDSMALGKLKERRARDGVDISRKRRQFSSPLCAQYGHDGSDCSIMADDNMSEDALGYM*