AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007636

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007636 MtrunA17_Chr2g0294911 96.700 606 19 1 1 605 1 606 0.0 1217
MsaG007636 MtrunA17_Chr4g0067981 62.225 683 171 14 1 600 2 680 0.0 762
MsaG007636 MtrunA17_Chr1g0161431 59.091 308 117 3 25 325 8 313 1.93e-115 357
MsaG007636 MtrunA17_Chr3g0137101 48.189 359 151 7 26 356 6 357 1.97e-101 321
MsaG007636 MtrunA17_Chr3g0123131 58.462 260 92 3 28 277 13 266 2.79e-95 303
MsaG007636 MtrunA17_Chr8g0376881 57.874 254 105 1 26 277 23 276 2.22e-92 295
MsaG007636 MtrunA17_Chr1g0149841 44.444 378 174 9 24 385 1 358 3.57e-92 293
MsaG007636 MtrunA17_Chr7g0275891 49.827 289 120 5 24 291 1 285 6.60e-85 269
MsaG007636 MtrunA17_Chr2g0304841 42.973 370 178 7 24 363 14 380 3.34e-84 277
MsaG007636 MtrunA17_Chr4g0074401 42.703 370 172 6 17 368 6 353 2.19e-82 270
MsaG007636 MtrunA17_Chr1g0149851 52.045 269 110 3 24 273 1 269 1.96e-79 261
MsaG007636 MtrunA17_Chr4g0074411 44.706 340 149 8 22 346 12 327 6.96e-79 261
MsaG007636 MtrunA17_Chr7g0275401 47.751 289 139 4 24 303 1 286 4.12e-75 241
MsaG007636 MtrunA17_Chr4g0074421 51.515 198 73 3 78 264 2 187 3.41e-59 196
MsaG007636 MtrunA17_Chr3g0134461 47.034 236 76 2 25 259 5 192 5.57e-59 195
MsaG007636 MtrunA17_Chr3g0134481 43.882 237 75 2 25 261 5 183 4.55e-57 190
MsaG007636 MtrunA17_Chr4g0009851 43.096 239 112 5 28 266 11 225 1.63e-56 193
MsaG007636 MtrunA17_Chr1g0149831 50.739 203 79 4 87 278 1 193 4.20e-56 189
MsaG007636 MtrunA17_Chr4g0009901 42.917 240 113 4 28 267 10 225 2.08e-55 189
MsaG007636 MtrunA17_Chr6g0471041 38.843 242 120 6 27 266 13 228 4.56e-45 165
MsaG007636 MtrunA17_Chr4g0009981 41.753 194 90 1 73 266 2 172 2.37e-44 158
MsaG007636 MtrunA17_Chr8g0391911 34.766 256 141 6 28 269 9 252 2.04e-35 134
MsaG007636 MtrunA17_Chr6g0473731 37.838 185 86 5 108 284 10 173 3.76e-29 120
MsaG007636 MtrunA17_Chr8g0383961 42.754 138 76 1 27 164 13 147 1.48e-28 115
MsaG007636 MtrunA17_Chr8g0350761 30.000 270 165 6 30 277 19 286 1.35e-27 116
MsaG007636 MtrunA17_Chr4g0011071 35.220 159 80 3 78 236 39 174 3.91e-27 108
MsaG007636 MtrunA17_Chr4g0031961 47.458 118 47 3 186 295 99 209 5.31e-24 103
MsaG007636 MtrunA17_Chr4g0009991 39.286 140 61 2 120 259 1 116 7.97e-23 94.4
MsaG007636 MtrunA17_Chr3g0127941 36.875 160 94 4 32 186 56 213 3.50e-22 101
MsaG007636 MtrunA17_Chr7g0276361 38.971 136 74 2 29 158 11 143 1.20e-21 99.4
MsaG007636 MtrunA17_Chr6g0456631 36.220 127 78 2 26 150 7 132 1.30e-21 92.4
MsaG007636 MtrunA17_Chr4g0030271 35.333 150 91 3 6 150 62 210 3.30e-21 98.2
MsaG007636 MtrunA17_Chr7g0255711 57.971 69 29 0 203 271 84 152 3.87e-21 94.4
MsaG007636 MtrunA17_Chr1g0181811 39.344 122 71 1 32 150 85 206 8.73e-21 97.1
MsaG007636 MtrunA17_Chr3g0091641 35.971 139 84 2 19 152 9 147 1.63e-20 95.5
MsaG007636 MtrunA17_Chr4g0061021 35.507 138 84 2 18 152 21 156 5.85e-19 90.9
MsaG007636 MtrunA17_Chr8g0345901 39.344 122 67 3 34 150 38 157 9.02e-19 90.5
MsaG007636 MtrunA17_Chr5g0400241 37.903 124 68 2 145 268 255 369 7.35e-17 84.0
MsaG007636 MtrunA17_Chr5g0400241 37.008 127 76 2 28 153 14 137 6.85e-14 74.7
MsaG007636 MtrunA17_Chr1g0198601 36.552 145 78 4 198 330 199 341 3.91e-16 80.5
MsaG007636 MtrunA17_Chr5g0423031 45.679 81 43 1 28 108 10 89 6.10e-16 76.3
MsaG007636 MtrunA17_Chr4g0010001 44.444 81 44 1 28 108 10 89 6.51e-16 73.6
MsaG007636 MtrunA17_Chr5g0417521 55.714 70 24 2 206 274 11 74 4.66e-15 77.0
MsaG007636 MtrunA17_Chr4g0040481 41.379 87 46 1 209 295 157 238 4.67e-15 77.8
MsaG007636 MtrunA17_Chr4g0054981 47.887 71 37 0 209 279 309 379 7.68e-15 77.8
MsaG007636 MtrunA17_Chr4g0054981 36.220 127 77 2 28 153 14 137 1.65e-13 73.6
MsaG007636 MtrunA17_Chr6g0469321 63.462 52 19 0 227 278 71 122 3.68e-14 71.2
MsaG007636 MtrunA17_Chr4g0042451 55.932 59 25 1 206 263 13 71 7.59e-14 73.2
MsaG007636 MtrunA17_Chr2g0324891 53.333 60 28 0 209 268 160 219 7.89e-14 73.9
MsaG007636 MtrunA17_Chr2g0321461 55.932 59 25 1 206 263 41 99 8.23e-14 73.6
MsaG007636 MtrunA17_Chr6g0461641 51.667 60 28 1 205 263 37 96 2.00e-13 72.4
MsaG007636 MtrunA17_Chr5g0422621 30.220 182 115 5 151 325 133 309 2.28e-13 72.0
MsaG007636 MtrunA17_Chr5g0402631 50.000 72 35 1 207 277 211 282 8.42e-13 70.1
MsaG007636 MtrunA17_Chr2g0310011 54.237 59 26 1 206 263 31 89 2.27e-12 68.9
MsaG007636 MtrunA17_Chr8g0356501 54.237 59 26 1 206 263 33 91 2.92e-12 68.6
MsaG007636 MtrunA17_Chr4g0045361 50.746 67 32 1 207 272 271 337 3.03e-12 68.9
MsaG007636 MtrunA17_Chr1g0197171 52.308 65 27 2 203 263 34 98 7.92e-12 66.6
MsaG007636 MtrunA17_Chr7g0274471 59.259 54 21 1 210 262 218 271 1.17e-11 67.0
MsaG007636 MtrunA17_Chr4g0062921 34.074 135 77 4 166 295 188 315 1.25e-11 67.0
MsaG007636 MtrunA17_Chr7g0261911 49.206 63 31 1 206 267 258 320 1.95e-11 66.6
MsaG007636 MtrunA17_Chr1g0190301 50.847 59 28 1 205 262 170 228 5.60e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007636 AT3G16857.2 46.999 683 241 21 21 600 26 690 2.14e-174 511
MsaG007636 AT3G16857.1 46.364 660 234 20 21 577 26 668 5.21e-163 481
MsaG007636 AT3G16857.3 44.671 638 232 21 66 600 1 620 1.13e-143 430
MsaG007636 AT2G01760.1 46.053 380 159 9 24 373 3 366 9.03e-96 298
MsaG007636 AT2G25180.1 59.924 262 93 3 26 281 11 266 9.05e-96 305
MsaG007636 AT2G01760.2 42.892 408 159 10 24 373 3 394 1.49e-89 283
MsaG007636 AT4G31920.1 47.143 350 142 8 24 354 9 334 1.56e-87 283
MsaG007636 AT1G67710.1 49.702 336 128 9 29 333 8 333 5.53e-86 278
MsaG007636 AT5G58080.1 46.875 320 128 7 26 324 12 310 6.33e-79 262
MsaG007636 AT2G01760.3 42.271 317 137 9 87 373 1 301 2.57e-62 209
MsaG007636 AT1G67710.2 45.683 278 110 9 87 333 1 268 1.64e-56 197
MsaG007636 AT5G49240.1 42.915 247 134 3 18 259 28 272 9.27e-54 185
MsaG007636 AT1G49190.1 35.254 295 149 7 27 296 29 306 8.74e-46 167
MsaG007636 AT3G62670.1 39.407 236 128 5 33 262 42 268 1.96e-45 167
MsaG007636 AT1G67710.3 41.473 258 110 9 107 333 12 259 1.16e-40 154
MsaG007636 AT5G07210.2 33.955 268 145 6 32 269 16 281 7.71e-39 151
MsaG007636 AT5G07210.1 32.971 276 145 7 32 269 16 289 2.77e-36 144
MsaG007636 AT2G27070.1 32.103 271 147 7 32 268 16 283 3.03e-33 134
MsaG007636 AT1G49190.2 44.800 125 69 0 27 151 29 153 2.86e-29 123
MsaG007636 AT5G59570.2 52.273 88 42 0 194 281 125 212 3.48e-24 103
MsaG007636 AT5G59570.1 52.273 88 42 0 194 281 125 212 3.48e-24 103
MsaG007636 AT3G46640.2 46.364 110 52 1 194 303 128 230 3.63e-23 101
MsaG007636 AT3G46640.1 46.364 110 52 1 194 303 128 230 3.63e-23 101
MsaG007636 AT2G46790.1 40.650 123 68 2 32 150 37 158 4.60e-23 103
MsaG007636 AT2G46790.3 40.650 123 68 2 32 150 37 158 4.80e-23 102
MsaG007636 AT3G46640.3 49.020 102 45 1 194 295 128 222 6.02e-23 100
MsaG007636 AT5G24470.1 39.200 125 71 2 31 151 49 172 5.08e-21 97.4
MsaG007636 AT5G60100.7 39.837 123 69 2 32 150 64 185 5.50e-21 96.3
MsaG007636 AT5G60100.6 39.837 123 69 2 32 150 64 185 5.50e-21 96.3
MsaG007636 AT5G60100.4 39.837 123 69 2 32 150 64 185 5.50e-21 96.3
MsaG007636 AT5G60100.5 39.837 123 69 2 32 150 64 185 6.82e-21 96.7
MsaG007636 AT5G60100.3 39.837 123 69 2 32 150 64 185 6.82e-21 96.7
MsaG007636 AT5G60100.1 39.837 123 69 2 32 150 64 185 6.82e-21 96.7
MsaG007636 AT2G40970.1 60.606 66 26 0 203 268 98 163 8.80e-21 92.4
MsaG007636 AT5G60100.2 39.837 123 69 2 32 150 64 185 1.71e-20 95.5
MsaG007636 AT5G05090.1 59.091 66 27 0 203 268 74 139 2.42e-20 91.7
MsaG007636 AT3G10760.1 59.091 66 27 0 203 268 98 163 3.78e-20 92.4
MsaG007636 AT5G61380.1 38.710 124 73 1 32 152 19 142 4.13e-20 95.1
MsaG007636 AT5G02810.1 40.164 122 70 1 32 150 78 199 5.38e-20 94.7
MsaG007636 AT5G44190.2 50.649 77 38 0 203 279 140 216 8.44e-18 85.9
MsaG007636 AT5G44190.1 50.649 77 38 0 203 279 140 216 1.06e-17 85.9
MsaG007636 AT2G20570.1 39.823 113 63 2 170 277 108 220 4.33e-16 81.3
MsaG007636 AT2G20570.2 38.060 134 77 2 145 277 108 236 2.15e-14 76.3
MsaG007636 AT3G04030.1 51.282 78 30 3 206 282 41 111 3.54e-14 75.1
MsaG007636 AT3G04030.3 51.282 78 30 3 206 282 41 111 4.17e-14 75.1
MsaG007636 AT5G18240.2 50.000 82 37 3 205 285 40 118 5.53e-14 74.7
MsaG007636 AT5G18240.3 50.000 82 37 3 205 285 40 118 5.53e-14 74.7
MsaG007636 AT5G18240.5 50.000 82 37 3 205 285 40 118 5.82e-14 74.7
MsaG007636 AT5G18240.1 50.000 82 37 3 205 285 40 118 5.89e-14 74.7
MsaG007636 AT5G18240.4 50.000 82 37 3 205 285 40 118 5.89e-14 74.7
MsaG007636 AT3G04030.2 48.052 77 33 2 206 282 41 110 4.88e-13 71.6
MsaG007636 AT4G18020.8 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
MsaG007636 AT4G18020.8 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
MsaG007636 AT4G18020.11 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
MsaG007636 AT4G18020.11 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
MsaG007636 AT4G18020.4 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
MsaG007636 AT4G18020.4 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
MsaG007636 AT4G18020.5 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
MsaG007636 AT4G18020.5 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
MsaG007636 AT4G18020.10 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
MsaG007636 AT4G18020.10 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
MsaG007636 AT4G18020.7 36.029 136 78 3 26 154 12 145 5.18e-13 72.0
MsaG007636 AT4G18020.7 51.613 62 29 1 207 268 294 354 6.93e-13 71.6
MsaG007636 AT4G18020.3 36.029 136 78 3 26 154 12 145 7.01e-13 72.0
MsaG007636 AT4G18020.3 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
MsaG007636 AT4G18020.2 36.029 136 78 3 26 154 12 145 7.01e-13 72.0
MsaG007636 AT4G18020.2 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
MsaG007636 AT4G18020.1 36.029 136 78 3 26 154 12 145 7.01e-13 72.0
MsaG007636 AT4G18020.1 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
MsaG007636 AT4G18020.6 36.029 136 78 3 26 154 12 145 7.01e-13 72.0
MsaG007636 AT4G18020.6 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
MsaG007636 AT4G18020.9 36.029 136 78 3 26 154 12 145 7.01e-13 72.0
MsaG007636 AT4G18020.9 51.613 62 29 1 207 268 294 354 7.38e-13 71.6
MsaG007636 AT1G69580.1 54.237 59 26 1 206 263 30 88 7.35e-13 70.5
MsaG007636 AT1G69580.2 54.237 59 26 1 206 263 30 88 7.54e-13 70.5
MsaG007636 AT3G24120.2 52.308 65 27 2 203 263 31 95 3.01e-12 68.2
MsaG007636 AT3G24120.1 52.308 65 27 2 203 263 31 95 3.31e-12 68.2
MsaG007636 AT1G79430.2 53.333 60 27 1 205 263 29 88 3.65e-12 68.6
MsaG007636 AT3G25790.1 38.655 119 61 4 214 330 200 308 3.86e-12 68.6
MsaG007636 AT4G13640.2 52.308 65 27 2 203 263 27 91 7.37e-12 67.0
MsaG007636 AT4G13640.1 52.308 65 27 2 203 263 27 91 7.48e-12 67.0
MsaG007636 AT1G49560.1 37.383 107 59 2 209 307 191 297 1.98e-11 66.2
MsaG007636 AT3G12730.1 49.275 69 28 2 202 263 9 77 2.72e-11 64.3
MsaG007636 AT1G25550.1 45.070 71 38 1 198 267 197 267 3.78e-11 65.5
MsaG007636 AT5G29000.4 45.714 70 35 2 196 262 215 284 5.82e-11 64.7
MsaG007636 AT1G68670.1 45.679 81 42 2 194 272 198 278 5.98e-11 65.1
MsaG007636 AT2G03500.1 47.059 68 35 1 207 273 230 297 8.68e-11 64.7

Find 146 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGCTTCATCAACAGTTTA+TGG 0.134096 2:-20919055 MsaT007636.1:CDS
CTTCAAACCCTTTCAGCTTC+TGG 0.197138 2:-20918163 MsaT007636.1:CDS
CCTTATCTCCGACCAGTTTC+CGG 0.220153 2:-20920093 MsaT007636.1:CDS
GTTGTTGTTGTTGAGTTTCA+TGG 0.237423 2:+20920160 None:intergenic
GATGTCTTGCCATCGATTTC+AGG 0.243060 2:-20917563 MsaT007636.1:CDS
CATGGATGGATTCAAACTTT+TGG 0.246707 2:-20919540 MsaT007636.1:CDS
GAAGCTACTTCGGGAATTAA+AGG 0.286940 2:-20917710 MsaT007636.1:CDS
AGTACGCAAGAGTCAATATT+TGG 0.295173 2:-20918211 MsaT007636.1:CDS
TGAGGTGTTGACCAACATTT+TGG 0.304132 2:+20917474 None:intergenic
CACCAGTCTGCTCAATCCTT+TGG 0.321223 2:-20917968 MsaT007636.1:CDS
GAGAACTCAAAACTAAGATT+TGG 0.328580 2:-20918085 MsaT007636.1:CDS
ACTTCGGGAATTAAAGGATT+AGG 0.335057 2:-20917704 MsaT007636.1:CDS
AAGAAGATGAAGCAGATGAT+AGG 0.337750 2:-20919122 MsaT007636.1:CDS
TTGAGCAGACTGGTGAAAAT+TGG 0.352834 2:+20917976 None:intergenic
TTCAGCTTCTGGCCAGTTCT+CGG 0.367341 2:-20918152 MsaT007636.1:CDS
TCGAGGCTTTGAAGAATATA+TGG 0.369544 2:-20919296 MsaT007636.1:CDS
CTTCGGGAATTAAAGGATTA+GGG 0.371380 2:-20917703 MsaT007636.1:CDS
ATATCCAGTAGCAGTATAAA+AGG 0.377177 2:-20917746 MsaT007636.1:CDS
AAACAATGCTTACTAATAAC+AGG 0.383212 2:+20919491 None:intergenic
CTATTGACTGATATTCCATT+TGG 0.387671 2:+20917794 None:intergenic
CACGGAGACCCGCCGGAAAC+TGG 0.388410 2:+20920081 None:intergenic
GGCAAGAGTGAAAGAATTCC+AGG 0.393464 2:-20917422 MsaT007636.1:CDS
TTCGGAAGAGAAAGAATAAC+TGG 0.397525 2:-20919263 MsaT007636.1:CDS
CTAGACTTTGCGCCGAGAAC+TGG 0.398375 2:+20918140 None:intergenic
TCATTTAGCATCTGACCTCT+TGG 0.399993 2:+20917872 None:intergenic
GATAATGAGTTTGACTTTGA+TGG 0.400817 2:-20917211 MsaT007636.1:CDS
AAACGTTGCCAGTCATCTTC+AGG 0.402049 2:-20918681 MsaT007636.1:intron
TAACAGGATCTGTTATAAAC+TGG 0.403457 2:+20917169 None:intergenic
ATTTGCTAATCCCCAATCAT+TGG 0.406561 2:+20917640 None:intergenic
CATGAGTGTGTTTCTGAACC+AGG 0.407623 2:-20917351 MsaT007636.1:intron
TGCTTCGGCTCAATGTTGAT+AGG 0.409175 2:+20918004 None:intergenic
CAAAGAAGCCTCGAGTTGTT+TGG 0.412556 2:-20919083 MsaT007636.1:CDS
AACCTCAGCTCTCTTACATT+TGG 0.414035 2:+20919631 None:intergenic
TTTCATGCGGAAGCTACTTC+GGG 0.415768 2:-20917719 MsaT007636.1:CDS
CCATCAGAAGTCCAATGATT+GGG 0.416956 2:-20917651 MsaT007636.1:CDS
TATTCAAGTTACTTTGCTGC+TGG 0.418842 2:+20918245 None:intergenic
TGAAAATTGGCAAACTGCTT+CGG 0.421606 2:+20917989 None:intergenic
ATGGAAATGATGAATGTTCC+TGG 0.426157 2:-20918716 MsaT007636.1:CDS
ACATCAATGTTTCCTTGTAA+AGG 0.437888 2:+20917581 None:intergenic
CTGAGCCATCTGCGTCAACC+AGG 0.458938 2:+20917901 None:intergenic
ATAAGGTTGCCGGAGTTTGA+TGG 0.460200 2:+20920110 None:intergenic
TATGGCTGTTGTGAATCAAT+TGG 0.460490 2:-20919037 MsaT007636.1:CDS
TCCATCAGAAGTCCAATGAT+TGG 0.463174 2:-20917652 MsaT007636.1:CDS
CGTAATGAATTGTCAACAAA+AGG 0.464558 2:+20917446 None:intergenic
TGATTAGAAAGAGAGCTAAC+AGG 0.464856 2:+20917153 None:intergenic
TGGAAAGATCTGAAGCAATC+TGG 0.465336 2:-20919243 MsaT007636.1:CDS
CTCTTGACTTTGATCAGAGA+AGG 0.465507 2:+20917379 None:intergenic
TTCTCGCAGCACAAAGGCGT+TGG 0.468445 2:-20917241 MsaT007636.1:intron
CCGGAAACTGGTCGGAGATA+AGG 0.470882 2:+20920093 None:intergenic
CCCAATCATTGGACTTCTGA+TGG 0.470889 2:+20917651 None:intergenic
TTGTCGAGTCTGTTGGATAG+AGG 0.472904 2:+20917526 None:intergenic
ATGGCTGTTGTGAATCAATT+GGG 0.473696 2:-20919036 MsaT007636.1:CDS
ATGCAACCTACAGGACCAAA+TGG 0.473881 2:-20917809 MsaT007636.1:CDS
ACATATGAGCAGCAGTAAAC+CGG 0.474063 2:-20918047 MsaT007636.1:CDS
ATTTCATGCGGAAGCTACTT+CGG 0.474676 2:-20917720 MsaT007636.1:CDS
CTAGCCAAGCTTCAAGCAGC+AGG 0.477941 2:-20918121 MsaT007636.1:CDS
GGAACATATTGGATTGGAGA+TGG 0.480393 2:-20919519 MsaT007636.1:CDS
AGAAGATGAAGCAGATGATA+GGG 0.481453 2:-20919121 MsaT007636.1:CDS
CAACATGCAAATCCTTTACA+AGG 0.491783 2:-20917593 MsaT007636.1:CDS
TTCCCTCTCAGATGCACTCC+GGG 0.493223 2:-20917770 MsaT007636.1:CDS
GTAGGTTGCATTAAAGATGA+TGG 0.494028 2:+20917821 None:intergenic
TACTCATCTTCAACGAATGA+AGG 0.499328 2:-20919171 MsaT007636.1:CDS
GATATTCCATTTGGTCCTGT+AGG 0.499380 2:+20917803 None:intergenic
CAAAACTAAGATTTGGAGAT+GGG 0.507173 2:-20918078 MsaT007636.1:CDS
CAAGGATCATGAGGCAAGTA+GGG 0.508406 2:+20920039 None:intergenic
CTGGTCGGAGATAAGGTTGC+CGG 0.509887 2:+20920100 None:intergenic
ATAATGAGTTTGACTTTGAT+GGG 0.511882 2:-20917210 MsaT007636.1:CDS
ATTCAAGTTACTTTGCTGCT+GGG 0.515195 2:+20918246 None:intergenic
GTTGAGAAGCATCATATGTC+AGG 0.515544 2:+20917612 None:intergenic
TCTTGACTTTGATCAGAGAA+GGG 0.515547 2:+20917380 None:intergenic
GATTGGGGATTAGCAAATAC+AGG 0.520281 2:-20917635 MsaT007636.1:CDS
TTCAAAGCCTCGATTCGCAC+AGG 0.523205 2:+20919306 None:intergenic
ATATATGGCAGCATGTGGTT+CGG 0.524503 2:-20919281 MsaT007636.1:CDS
TCAAGGATCATGAGGCAAGT+AGG 0.525643 2:+20920038 None:intergenic
GCTTCGGCTCAATGTTGATA+GGG 0.528322 2:+20918005 None:intergenic
TTTAAGCGATGTGCATATGC+CGG 0.529451 2:-20919564 MsaT007636.1:CDS
CCGCATATTTGCGCTGCCAA+AGG 0.530696 2:+20917952 None:intergenic
AAACCGGTGAACTTTCTTCA+CGG 0.535622 2:-20918031 MsaT007636.1:CDS
CGATGTGCATATGCCGGACA+TGG 0.535790 2:-20919558 MsaT007636.1:CDS
ATACAAATTCTCATGCTACA+AGG 0.536556 2:-20917847 MsaT007636.1:CDS
CTTTGGCAGCGCAAATATGC+GGG 0.536705 2:-20917951 MsaT007636.1:CDS
TCAAAACTAAGATTTGGAGA+TGG 0.536886 2:-20918079 MsaT007636.1:CDS
TTGTATCTTCGAAGGTTGAG+CGG 0.540940 2:-20918274 MsaT007636.1:CDS
GAACTGGCCAGAAGCTGAAA+GGG 0.541735 2:+20918156 None:intergenic
AGTTTGAATCCATCCATGTC+CGG 0.543637 2:+20919545 None:intergenic
ATCTCCGACCAGTTTCCGGC+GGG 0.544362 2:-20920089 MsaT007636.1:CDS
AAGCCCCTGGTTGACGCAGA+TGG 0.544982 2:-20917906 MsaT007636.1:CDS
AATACCGTTTGTATCTTCGA+AGG 0.548651 2:-20918282 MsaT007636.1:CDS
TTAATCTCGATGTTTGTACC+TGG 0.554637 2:+20917404 None:intergenic
TCATCTTTAATGCAACCTAC+AGG 0.556863 2:-20917818 MsaT007636.1:CDS
GTGCATATGCCGGACATGGA+TGG 0.559547 2:-20919554 MsaT007636.1:CDS
TTTCCCTCTCAGATGCACTC+CGG 0.561462 2:-20917771 MsaT007636.1:CDS
ATATCATACCTGAAGATGAC+TGG 0.562314 2:+20918673 None:intergenic
TGAGCCATCTGCGTCAACCA+GGG 0.564665 2:+20917902 None:intergenic
AGAACTGGCCAGAAGCTGAA+AGG 0.565004 2:+20918155 None:intergenic
ATAGTGATGTCGGCTGATGA+TGG 0.565866 2:-20919384 MsaT007636.1:intron
TGACTCTTGCGTACTCATGA+AGG 0.572243 2:+20918219 None:intergenic
ATTCCGTGAAGAAAGTTCAC+CGG 0.576781 2:+20918028 None:intergenic
TGCCAAAGGATTGAGCAGAC+TGG 0.579512 2:+20917966 None:intergenic
CAAGGTGATCACCAAAATGT+TGG 0.586348 2:-20917485 MsaT007636.1:CDS
GCAAGAGCTCAAAGAAGAGG+AGG 0.587165 2:-20919149 MsaT007636.1:CDS
CTTCTCTGATCAAAGTCAAG+AGG 0.587257 2:-20917378 MsaT007636.1:CDS
ACAAGTACACGGAGACCCGC+CGG 0.589774 2:+20920074 None:intergenic
GAGCCATCTGCGTCAACCAG+GGG 0.591634 2:+20917903 None:intergenic
CCACGATGGCCTGAAATCGA+TGG 0.592221 2:+20917554 None:intergenic
AAGTCCTGCTGCTTGAAGCT+TGG 0.592461 2:+20918117 None:intergenic
GAATGTGTTTATAGTGATGT+CGG 0.592942 2:-20919394 MsaT007636.1:intron
TGTCAACAAAAGGTGTAATG+AGG 0.594092 2:+20917456 None:intergenic
AGATGAGTAATTAGCATCAG+TGG 0.594629 2:+20919185 None:intergenic
AAGATCAATTTCATTTACAG+AGG 0.595752 2:+20918183 None:intergenic
CCTTTGGCAGCGCAAATATG+CGG 0.597795 2:-20917952 MsaT007636.1:CDS
TGGTGAAGTTGAGGTTGACA+TGG 0.599570 2:+20920130 None:intergenic
CTTGACTTTGATCAGAGAAG+GGG 0.600312 2:+20917381 None:intergenic
TACCCGGAGTGCATCTGAGA+GGG 0.609734 2:+20917768 None:intergenic
AATAGAGATGGTATGAGTCA+AGG 0.612883 2:-20917503 MsaT007636.1:CDS
AATCAAACCTGTGCGAATCG+AGG 0.614923 2:-20919313 MsaT007636.1:CDS
TCAACTTCACCATCAAACTC+CGG 0.617812 2:-20920119 MsaT007636.1:CDS
CAGACTCGACAAAATAGAGA+TGG 0.622886 2:-20917515 MsaT007636.1:CDS
CATCAGAAGTCCAATGATTG+GGG 0.623338 2:-20917650 MsaT007636.1:CDS
CCATCGATTTCAGGCCATCG+TGG 0.623771 2:-20917554 MsaT007636.1:CDS
AAGATTGAAGACCTTCATAG+AGG 0.625198 2:+20919997 None:intergenic
GCATTCTTTCAAGGATCATG+AGG 0.625199 2:+20920030 None:intergenic
CTGCTGCAAAGAGAAGCCCC+TGG 0.625822 2:-20917919 MsaT007636.1:CDS
TGGAAATGATGAATGTTCCT+GGG 0.628797 2:-20918715 MsaT007636.1:CDS
AAAGATCTGAAGCAATCTGG+TGG 0.628824 2:-20919240 MsaT007636.1:CDS
TATCTCCGACCAGTTTCCGG+CGG 0.629363 2:-20920090 MsaT007636.1:CDS
GAAGAATATATGGCAGCATG+TGG 0.629609 2:-20919286 MsaT007636.1:CDS
TCAACCTTCGAAGATACAAA+CGG 0.630642 2:+20918278 None:intergenic
TTCGGGAATTAAAGGATTAG+GGG 0.632056 2:-20917702 MsaT007636.1:CDS
TTGTATTTCTCGCAGCACAA+AGG 0.634173 2:-20917247 MsaT007636.1:intron
AAGGCAAGAGCTCAAAGAAG+AGG 0.635070 2:-20919152 MsaT007636.1:CDS
ACGCAAGAGTCAATATTTGG+AGG 0.635081 2:-20918208 MsaT007636.1:CDS
TCGGGAATTAAAGGATTAGG+GGG 0.636049 2:-20917701 MsaT007636.1:CDS
TCAATAGACCAAACAACTCG+AGG 0.639848 2:+20919075 None:intergenic
GGAGTTTGATGGTGAAGTTG+AGG 0.651951 2:+20920121 None:intergenic
CATCATCAACAACAAGTACA+CGG 0.653506 2:+20920063 None:intergenic
GAGACCCGCCGGAAACTGGT+CGG 0.661705 2:+20920085 None:intergenic
CAAGAGCTCAAAGAAGAGGA+GGG 0.663100 2:-20919148 MsaT007636.1:CDS
TTTGTTGACAATTCATTACG+GGG 0.665334 2:-20917443 MsaT007636.1:CDS
TACCAAATGTAAGAGAGCTG+AGG 0.673543 2:-20919633 MsaT007636.1:CDS
ATACTGCTACTGGATATACC+CGG 0.681469 2:+20917752 None:intergenic
TATAAAAGGTGCATTTCATG+CGG 0.685544 2:-20917732 MsaT007636.1:CDS
ATACCCGGAGTGCATCTGAG+AGG 0.700149 2:+20917767 None:intergenic
ATGGCTCAGTCTCAACCAAG+AGG 0.702081 2:-20917887 MsaT007636.1:CDS
GTAATGAAAGGTGTGACACA+CGG 0.719143 2:-20919351 MsaT007636.1:CDS
ATAAACTGGAATGTTGTCTG+CGG 0.732217 2:+20917183 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 20917168 20920180 20917168 ID=MsaG007636
Chr2 mRNA 20917168 20920180 20917168 ID=MsaT007636.1;Parent=MsaG007636
Chr2 exon 20917168 20917254 20917168 ID=MsaT007636.1.exon6;Parent=MsaT007636.1
Chr2 CDS 20917168 20917254 20917168 ID=cds.MsaT007636.1;Parent=MsaT007636.1
Chr2 exon 20917352 20918305 20917352 ID=MsaT007636.1.exon5;Parent=MsaT007636.1
Chr2 CDS 20917352 20918305 20917352 ID=cds.MsaT007636.1;Parent=MsaT007636.1
Chr2 exon 20918682 20918758 20918682 ID=MsaT007636.1.exon4;Parent=MsaT007636.1
Chr2 CDS 20918682 20918758 20918682 ID=cds.MsaT007636.1;Parent=MsaT007636.1
Chr2 exon 20919028 20919402 20919028 ID=MsaT007636.1.exon3;Parent=MsaT007636.1
Chr2 CDS 20919028 20919402 20919028 ID=cds.MsaT007636.1;Parent=MsaT007636.1
Chr2 exon 20919504 20919656 20919504 ID=MsaT007636.1.exon2;Parent=MsaT007636.1
Chr2 CDS 20919504 20919656 20919504 ID=cds.MsaT007636.1;Parent=MsaT007636.1
Chr2 exon 20920009 20920180 20920009 ID=MsaT007636.1.exon1;Parent=MsaT007636.1
Chr2 CDS 20920009 20920180 20920009 ID=cds.MsaT007636.1;Parent=MsaT007636.1
Gene Sequence

>MsaT007636.1

ATGAAACTCAACAACAACAACAAAATTTCCATGTCAACCTCAACTTCACCATCAAACTCCGGCAACCTTATCTCCGACCAGTTTCCGGCGGGTCTCCGTGTACTTGTTGTTGATGATGACCCTACTTGCCTCATGATCCTTGAAAGAATGCTCCGTTTTTGCCTCTATGAAGTTACCAAATGTAAGAGAGCTGAGGTTGCATTATCACTTCTTAGAGAGAATAAAAATGGGTTCGACATTGTTTTAAGCGATGTGCATATGCCGGACATGGATGGATTCAAACTTTTGGAACATATTGGATTGGAGATGGATCTTCCTGTTATTATGATGTCGGCTGATGATGGAAAAAACGTGGTAATGAAAGGTGTGACACACGGTGCTTGTGATTATCTAATCAAACCTGTGCGAATCGAGGCTTTGAAGAATATATGGCAGCATGTGGTTCGGAAGAGAAAGAATAACTGGAAAGATCTGAAGCAATCTGGTGGATTAGATGAAAGAGATCGAGAAAAAATTGTGTCCACTGATGCTAATTACTCATCTTCAACGAATGAAGGCAAGAGCTCAAAGAAGAGGAGGGATGAAGAAGATGAAGCAGATGATAGGGATGATTGTTCGACGTCAAAGAAGCCTCGAGTTGTTTGGTCTATTGAGCTTCATCAACAGTTTATGGCTGTTGTGAATCAATTGGGATTAGACAAGGCTGTTCCTAAAAAGATTATGGAAATGATGAATGTTCCTGGGCTCACTAGAGAAAACGTTGCCAGTCATCTTCAGAAATACCGTTTGTATCTTCGAAGGTTGAGCGGAGTTTCCCAGCAGCAAAGTAACTTGAATAACTCCTTCATGAGTACGCAAGAGTCAATATTTGGAGGAACCTCTGTAAATGAAATTGATCTTCAAACCCTTTCAGCTTCTGGCCAGTTCTCGGCGCAAAGTCTAGCCAAGCTTCAAGCAGCAGGACTTTACAGTCCTGAGAACTCAAAACTAAGATTTGGAGATGGGCAACTGCAACATATGAGCAGCAGTAAACCGGTGAACTTTCTTCACGGAATCCCTATCAACATTGAGCCGAAGCAGTTTGCCAATTTTCACCAGTCTGCTCAATCCTTTGGCAGCGCAAATATGCGGGTCAATGCTTCTGCTGCAAAGAGAAGCCCCTGGTTGACGCAGATGGCTCAGTCTCAACCAAGAGGTCAGATGCTAAATGAGAATACAAATTCTCATGCTACAAGGTTTCCATCATCTTTAATGCAACCTACAGGACCAAATGGAATATCAGTCAATAGTTTCCCTCTCAGATGCACTCCGGGTATATCCAGTAGCAGTATAAAAGGTGCATTTCATGCGGAAGCTACTTCGGGAATTAAAGGATTAGGGGGATTTAGTTATGATGTTTATAATGAACTCCATCAGAAGTCCAATGATTGGGGATTAGCAAATACAGGCCTGACATATGATGCTTCTCAACATGCAAATCCTTTACAAGGAAACATTGATGTCTTGCCATCGATTTCAGGCCATCGTGGTTTTTCCTCTATCCAACAGACTCGACAAAATAGAGATGGTATGAGTCAAGGTGATCACCAAAATGTTGGTCAACACCTCATTACACCTTTTGTTGACAATTCATTACGGGGCAAGAGTGAAAGAATTCCAGGTACAAACATCGAGATTAACCCCTTCTCTGATCAAAGTCAAGAGGATCTCATGAGTGTGTTTCTGAACCAGCACAAAGGCGTTGGACAAGTTGATAATGAGTTTGACTTTGATGGGTATTCCGCAGACAACATTCCAGTTTATAACAGATCCTGTTAG
Protein sequence

>MsaT007636.1

MKLNNNNKISMSTSTSPSNSGNLISDQFPAGLRVLVVDDDPTCLMILERMLRFCLYEVTKCKRAEVALSLLRENKNGFDIVLSDVHMPDMDGFKLLEHIGLEMDLPVIMMSADDGKNVVMKGVTHGACDYLIKPVRIEALKNIWQHVVRKRKNNWKDLKQSGGLDERDREKIVSTDANYSSSTNEGKSSKKRRDEEDEADDRDDCSTSKKPRVVWSIELHQQFMAVVNQLGLDKAVPKKIMEMMNVPGLTRENVASHLQKYRLYLRRLSGVSQQQSNLNNSFMSTQESIFGGTSVNEIDLQTLSASGQFSAQSLAKLQAAGLYSPENSKLRFGDGQLQHMSSSKPVNFLHGIPINIEPKQFANFHQSAQSFGSANMRVNASAAKRSPWLTQMAQSQPRGQMLNENTNSHATRFPSSLMQPTGPNGISVNSFPLRCTPGISSSSIKGAFHAEATSGIKGLGGFSYDVYNELHQKSNDWGLANTGLTYDASQHANPLQGNIDVLPSISGHRGFSSIQQTRQNRDGMSQGDHQNVGQHLITPFVDNSLRGKSERIPGTNIEINPFSDQSQEDLMSVFLNQHKGVGQVDNEFDFDGYSADNIPVYNRSC*