AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007716


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007716 MtrunA17_Chr2g0295731 95.357 280 13 0 1 280 102 381 0.0 548
MsaG007716 MtrunA17_Chr2g0296151 70.000 280 84 0 1 280 95 374 4.02e-137 392
MsaG007716 MtrunA17_Chr2g0296121 68.929 280 87 0 1 280 181 460 2.04e-131 380
MsaG007716 MtrunA17_Chr2g0295741 65.357 280 97 0 1 280 101 380 1.52e-126 365
MsaG007716 MtrunA17_Chr2g0296111 65.125 281 95 2 2 280 95 374 6.92e-125 361
MsaG007716 MtrunA17_Chr4g0067121 51.957 281 134 1 1 280 104 384 1.52e-101 301
MsaG007716 MtrunA17_Chr2g0296711 50.883 283 136 2 1 280 108 390 9.45e-95 286
MsaG007716 MtrunA17_Chr2g0296741 50.175 285 135 2 1 280 125 407 1.41e-93 282
MsaG007716 MtrunA17_Chr2g0296221 51.579 285 133 3 1 280 134 418 1.60e-93 283
MsaG007716 MtrunA17_Chr2g0296231 50.896 279 132 3 1 276 104 380 3.34e-91 275
MsaG007716 MtrunA17_Chr2g0296721 48.227 282 144 1 1 280 105 386 3.45e-89 270
MsaG007716 MtrunA17_Chr2g0296171 51.957 281 130 3 1 276 115 395 7.75e-89 270
MsaG007716 MtrunA17_Chr2g0296251 46.395 319 123 3 1 276 85 398 4.65e-88 267
MsaG007716 MtrunA17_Chr2g0315731 48.432 287 135 2 2 280 126 407 6.98e-88 268
MsaG007716 MtrunA17_Chr2g0296291 44.340 318 139 2 1 280 85 402 1.48e-86 264
MsaG007716 MtrunA17_Chr2g0296191 50.719 278 132 3 1 273 717 994 1.08e-83 271
MsaG007716 MtrunA17_Chr2g0296191 47.552 286 128 3 1 275 235 509 9.91e-82 265
MsaG007716 MtrunA17_Chr2g0296301 44.765 277 151 1 1 275 89 365 3.40e-82 252
MsaG007716 MtrunA17_Chr2g0295761 45.487 277 150 1 1 276 113 389 4.78e-81 249
MsaG007716 MtrunA17_Chr2g0296161 67.901 162 52 0 119 280 1 162 2.77e-76 229
MsaG007716 MtrunA17_Chr2g0286191 44.286 280 152 3 1 278 84 361 2.60e-74 231
MsaG007716 MtrunA17_Chr2g0296131 66.456 158 53 0 123 280 9 166 1.03e-72 221
MsaG007716 MtrunA17_Chr4g0067141 42.336 274 158 0 1 274 97 370 3.77e-72 226
MsaG007716 MtrunA17_Chr2g0296551 55.435 184 82 0 97 280 5 188 9.43e-70 214
MsaG007716 MtrunA17_Chr4g0067671 42.529 261 150 0 1 261 97 357 1.53e-69 219
MsaG007716 MtrunA17_Chr2g0296181 45.520 279 122 3 1 276 106 357 2.07e-69 219
MsaG007716 MtrunA17_Chr2g0296211 53.571 196 88 1 88 280 167 362 1.68e-64 206
MsaG007716 MtrunA17_Chr2g0295721 85.345 116 17 0 119 234 1 116 1.76e-64 197
MsaG007716 MtrunA17_Chr2g0296701 57.862 159 67 0 122 280 2 160 5.53e-60 188
MsaG007716 MtrunA17_Chr2g0296751 61.600 125 48 0 152 276 5 129 7.46e-48 157
MsaG007716 MtrunA17_Chr2g0286221 50.000 160 78 2 121 280 16 173 3.09e-47 156
MsaG007716 MtrunA17_Chr2g0295711 73.684 95 25 0 1 95 101 195 3.59e-39 136
MsaG007716 MtrunA17_Chr4g0001831 29.930 284 192 4 1 278 90 372 2.87e-34 127
MsaG007716 MtrunA17_Chr8g0377781 28.148 270 190 3 4 270 100 368 4.73e-27 108
MsaG007716 MtrunA17_Chr2g0295751 70.667 75 22 0 1 75 102 176 1.84e-25 105
MsaG007716 MtrunA17_Chr4g0067131 50.617 81 40 0 192 272 1 81 6.29e-24 92.8
MsaG007716 MtrunA17_Chr8g0377801 25.271 277 204 2 1 274 109 385 1.98e-23 98.6
MsaG007716 MtrunA17_Chr4g0067681 55.738 61 27 0 181 241 1 61 8.15e-17 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007716 AT5G07900.1 34.409 279 177 3 1 273 121 399 2.60e-50 171
MsaG007716 AT1G21150.3 28.772 285 193 5 1 277 72 354 2.35e-32 122
MsaG007716 AT1G21150.1 28.772 285 193 5 1 277 108 390 4.44e-32 122
MsaG007716 AT1G21150.2 28.772 285 193 5 1 277 172 454 9.20e-32 122
MsaG007716 AT1G61980.3 26.861 309 175 7 1 265 104 405 1.38e-25 105
MsaG007716 AT1G61980.2 26.861 309 175 7 1 265 104 405 1.38e-25 105
MsaG007716 AT1G61980.1 26.861 309 175 7 1 265 104 405 1.38e-25 105
MsaG007716 AT5G64950.1 29.455 275 175 5 6 265 96 366 1.51e-22 96.3
MsaG007716 AT1G61970.2 26.087 299 178 6 1 259 104 399 1.03e-21 94.4
MsaG007716 AT1G61970.7 26.087 299 178 6 1 259 104 399 1.03e-21 94.4
MsaG007716 AT1G61970.6 26.087 299 178 6 1 259 104 399 1.03e-21 94.4
MsaG007716 AT1G61970.3 26.087 299 178 6 1 259 104 399 1.03e-21 94.4
MsaG007716 AT1G61970.5 26.087 299 178 6 1 259 104 399 1.03e-21 94.4
MsaG007716 AT1G61970.4 26.087 299 178 6 1 259 104 399 1.03e-21 94.4
MsaG007716 AT1G61970.1 26.087 299 178 6 1 259 104 399 1.03e-21 94.4
MsaG007716 AT1G62010.1 24.648 284 173 6 20 265 109 389 1.32e-20 91.3
MsaG007716 AT1G62120.1 25.407 307 184 7 1 265 108 411 5.43e-20 89.4
MsaG007716 AT1G61990.2 25.868 317 179 9 1 273 104 408 7.39e-18 83.2
MsaG007716 AT1G61990.1 25.868 317 179 9 1 273 104 408 7.39e-18 83.2
MsaG007716 AT3G46950.1 29.293 198 123 3 1 191 104 291 2.03e-15 76.3
MsaG007716 AT1G61960.1 28.652 178 125 2 1 176 104 281 6.22e-13 68.9
MsaG007716 AT1G62110.1 26.263 198 131 3 1 189 104 295 8.36e-13 68.6
MsaG007716 AT1G62085.1 28.571 182 121 4 1 176 107 285 1.06e-11 65.1
MsaG007716 AT1G62085.2 28.571 182 121 4 1 176 107 285 1.06e-11 65.1

Find 46 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATCTGACAAGGAAATTT+TGG 0.116874 2:+22125984 MsaT007716.1:CDS
TAAATTTAGTGATGAACTTT+TGG 0.199642 2:+22126355 MsaT007716.1:CDS
GTAAAGGAATTAAAGGATTT+GGG 0.236314 2:+22126159 MsaT007716.1:CDS
AGACCAACCCCATTTCTTAA+AGG 0.251614 2:-22126268 None:intergenic
GGTATTGTTTACTGTTGTTT+TGG 0.253046 2:-22126223 None:intergenic
GGTAAAGGAATTAAAGGATT+TGG 0.272443 2:+22126158 MsaT007716.1:CDS
AAACTTAATGACAAAATATA+AGG 0.279924 2:-22126420 None:intergenic
TGACAAAATATAAGGTTGTT+TGG 0.318444 2:-22126412 None:intergenic
AAACTTTGGCAACACCGTTT+TGG 0.327649 2:-22125833 None:intergenic
CAAGTCGGTAAAGGAATTAA+AGG 0.349745 2:+22126152 MsaT007716.1:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2:+22126374 MsaT007716.1:CDS
AAGTTGATGCCTTTAAGAAA+TGG 0.366199 2:+22126259 MsaT007716.1:CDS
AGTTGATGCCTTTAAGAAAT+GGG 0.371481 2:+22126260 MsaT007716.1:CDS
AACTTATCAGTCACTACAAA+TGG 0.416436 2:-22126528 None:intergenic
GTCAGAGGTAGAAGCACCTT+TGG 0.417863 2:-22125869 None:intergenic
CATCAACTTTCTCTTTCCAT+AGG 0.426803 2:-22126245 None:intergenic
GGTTTGCAGGAAAGCAGCTC+TGG 0.441213 2:-22125808 None:intergenic
AATAGTCCCAGCCTATGAAT+TGG 0.461317 2:+22125948 MsaT007716.1:CDS
TCACAATGCTGATTCAGAAT+GGG 0.468303 2:+22126061 MsaT007716.1:CDS
ATCACAATGCTGATTCAGAA+TGG 0.471702 2:+22126060 MsaT007716.1:CDS
GGAAAGCAGCTCTGGCATCT+TGG 0.471973 2:-22125800 None:intergenic
GAATCTGTACACCAATTCAT+AGG 0.473956 2:-22125959 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.480856 2:+22126373 MsaT007716.1:CDS
CTGTACACCAATTCATAGGC+TGG 0.485721 2:-22125955 None:intergenic
CTGGGACTATTTGATTCTTG+AGG 0.490835 2:-22125936 None:intergenic
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2:+22126383 MsaT007716.1:CDS
AATATAAGGTTGTTTGGCAA+TGG 0.508469 2:-22126406 None:intergenic
ATCAACTTTCTCTTTCCATA+GGG 0.521600 2:-22126244 None:intergenic
CAACAGTAAACAATACCCTA+TGG 0.530932 2:+22126229 MsaT007716.1:CDS
TGACATGCTCAAGTCGGTAA+AGG 0.531848 2:+22126143 MsaT007716.1:CDS
GTGCAATTTCTTCTCAACAA+AGG 0.536369 2:+22126474 MsaT007716.1:CDS
TTCCTGCAAACCCTCCAAAA+CGG 0.545667 2:+22125819 MsaT007716.1:CDS
AATTGCACAACAGCGGCCCT+CGG 0.562081 2:-22126459 None:intergenic
AGGCTTACATACAAGACTCT+AGG 0.563542 2:-22125916 None:intergenic
TAAAGGAATTAAAGGATTTG+GGG 0.564846 2:+22126160 MsaT007716.1:CDS
CATTGCTGAGGGAACAGACT+CGG 0.569500 2:+22126106 MsaT007716.1:CDS
GTTGATGCCTTTAAGAAATG+GGG 0.573670 2:+22126261 MsaT007716.1:CDS
CAGATTCTTGCAATCTGACA+AGG 0.575407 2:+22125975 MsaT007716.1:CDS
AAACATTAAATCATTGCTGA+GGG 0.598387 2:+22126095 MsaT007716.1:CDS
ATGCCTTTAAGAAATGGGGT+TGG 0.598819 2:+22126265 MsaT007716.1:CDS
TGTACACCAATTCATAGGCT+GGG 0.603503 2:-22125954 None:intergenic
CACAATGCTGATTCAGAATG+GGG 0.608246 2:+22126062 MsaT007716.1:CDS
CAAACATTAAATCATTGCTG+AGG 0.611140 2:+22126094 MsaT007716.1:CDS
GACAATGTTAACAATGTCAG+AGG 0.628038 2:-22125884 None:intergenic
GAGAAGAAATTGCACAACAG+CGG 0.635644 2:-22126466 None:intergenic
GAGACGTGACATGCTCAAGT+CGG 0.669830 2:+22126137 MsaT007716.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22125793 22126635 22125793 ID=MsaG007716
Chr2 mRNA 22125793 22126635 22125793 ID=MsaT007716.1;Parent=MsaG007716
Chr2 exon 22125793 22126635 22125793 ID=MsaT007716.1.exon1;Parent=MsaT007716.1
Chr2 CDS 22125793 22126635 22125793 ID=cds.MsaT007716.1;Parent=MsaT007716.1
Gene Sequence

>MsaT007716.1

ATGATTGCCAAGATGCCAGAGCTGCTTTCCTGCAAACCCTCCAAAACGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTACCTCTGACATTGTTAACATTGTCAGTAAGACTCCTAGAGTCTTGTATGTAAGCCTCAAGAATCAAATAGTCCCAGCCTATGAATTGGTGTACAGATTCTTGCAATCTGACAAGGAAATTTTGGAAAAGTTGAATTACAGTGCAAATTTACTTTGTGATTGTACTGTGCAAAATAACATCACAATGCTGATTCAGAATGGGGTGTCTGACTCAAACATTAAATCATTGCTGAGGGAACAGACTCGGATATTCAAGAGACGTGACATGCTCAAGTCGGTAAAGGAATTAAAGGATTTGGGGTTTAATCCTTCAAAAACAACTTTTGGTGTAGCATTGCATGCCAAAACAACAGTAAACAATACCCTATGGAAAGAGAAAGTTGATGCCTTTAAGAAATGGGGTTGGTCTGAAGAAGATTCTCTCGAAGCATTTAGAAAGAAATCTTATTCTATGTTAACATCCATTGATAAAATAAATTTAGTGATGAACTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCCAAACAACCTTATATTTTGTCATTAAGTTTGGAAAAAAGGATCATTCCGAGGGCCGCTGTTGTGCAATTTCTTCTCAACAAAGGTTTGACAGATAAGAACGCAAGCTTAACTTATCCATTTGTAGTGACTGATAAGTTGTTTCTTAATATGTTTATAAAACGTTTTGAGAATGAGGCTTGTTATCTTTTAAAGTTGTATGAGGAAAAACGCAATCTAGGATAG

Protein sequence

>MsaT007716.1

MIAKMPELLSCKPSKTVLPKFQFFLSKGASTSDIVNIVSKTPRVLYVSLKNQIVPAYELVYRFLQSDKEILEKLNYSANLLCDCTVQNNITMLIQNGVSDSNIKSLLREQTRIFKRRDMLKSVKELKDLGFNPSKTTFGVALHAKTTVNNTLWKEKVDAFKKWGWSEEDSLEAFRKKSYSMLTSIDKINLVMNFWVNQLGWDAMAIAKQPYILSLSLEKRIIPRAAVVQFLLNKGLTDKNASLTYPFVVTDKLFLNMFIKRFENEACYLLKLYEEKRNLG*