AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007717

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007717 MtrunA17_Chr2g0295741 93.830 389 24 0 1 389 1 389 0.0 751
MsaG007717 MtrunA17_Chr2g0296151 66.753 385 122 2 1 385 1 379 0.0 511
MsaG007717 MtrunA17_Chr2g0296111 67.838 370 115 3 23 389 15 383 2.70e-177 498
MsaG007717 MtrunA17_Chr2g0295731 64.599 387 122 3 1 379 1 380 2.39e-174 491
MsaG007717 MtrunA17_Chr2g0296121 64.962 391 135 2 1 389 79 469 3.03e-174 494
MsaG007717 MtrunA17_Chr2g0296221 51.571 382 175 4 17 388 45 426 3.75e-125 368
MsaG007717 MtrunA17_Chr2g0296251 49.745 392 155 4 25 376 9 398 6.56e-124 363
MsaG007717 MtrunA17_Chr2g0296741 50.541 370 173 3 28 388 47 415 2.45e-122 360
MsaG007717 MtrunA17_Chr2g0296721 50.949 369 172 3 28 388 27 394 2.90e-121 357
MsaG007717 MtrunA17_Chr2g0296711 51.453 344 164 1 48 388 55 398 2.06e-120 355
MsaG007717 MtrunA17_Chr2g0315731 49.593 369 176 3 29 388 48 415 2.84e-118 350
MsaG007717 MtrunA17_Chr2g0296171 50.667 375 171 4 16 376 21 395 3.33e-117 347
MsaG007717 MtrunA17_Chr4g0067121 48.116 345 176 2 42 385 47 389 2.82e-115 341
MsaG007717 MtrunA17_Chr2g0296291 46.173 405 174 5 25 388 9 410 1.06e-114 340
MsaG007717 MtrunA17_Chr2g0296231 47.606 376 189 4 17 388 21 392 4.60e-114 338
MsaG007717 MtrunA17_Chr2g0296181 49.602 377 159 4 17 389 21 370 8.29e-114 337
MsaG007717 MtrunA17_Chr2g0296301 47.922 361 176 5 30 383 18 373 1.12e-112 334
MsaG007717 MtrunA17_Chr2g0296191 51.226 367 169 4 17 373 628 994 7.20e-112 350
MsaG007717 MtrunA17_Chr2g0296191 50.267 374 173 4 7 375 144 509 5.11e-110 345
MsaG007717 MtrunA17_Chr2g0295761 49.419 344 171 2 37 379 51 392 9.45e-110 327
MsaG007717 MtrunA17_Chr4g0067141 44.444 342 186 2 35 374 31 370 1.65e-93 285
MsaG007717 MtrunA17_Chr4g0067671 46.273 322 171 1 40 361 38 357 1.14e-90 277
MsaG007717 MtrunA17_Chr2g0286191 44.880 332 177 4 47 376 32 359 9.53e-88 270
MsaG007717 MtrunA17_Chr2g0296131 68.786 173 54 0 216 388 2 174 6.92e-85 256
MsaG007717 MtrunA17_Chr2g0296161 67.879 165 53 0 221 385 3 167 4.72e-75 230
MsaG007717 MtrunA17_Chr2g0295711 60.396 202 66 3 1 195 1 195 8.61e-73 226
MsaG007717 MtrunA17_Chr2g0295751 71.429 161 46 0 12 172 13 173 1.56e-70 229
MsaG007717 MtrunA17_Chr2g0296551 55.729 192 85 0 197 388 5 196 1.94e-70 219
MsaG007717 MtrunA17_Chr2g0296211 52.195 205 95 1 187 388 166 370 6.81e-65 211
MsaG007717 MtrunA17_Chr2g0296701 58.434 166 69 0 223 388 3 168 3.36e-63 200
MsaG007717 MtrunA17_Chr2g0296751 61.702 141 53 1 248 388 1 140 2.96e-55 179
MsaG007717 MtrunA17_Chr2g0295721 70.536 112 33 0 223 334 5 116 1.32e-50 166
MsaG007717 MtrunA17_Chr4g0001831 30.172 348 232 6 34 374 25 368 1.23e-44 158
MsaG007717 MtrunA17_Chr2g0286221 45.122 164 85 3 216 376 8 169 7.27e-40 140
MsaG007717 MtrunA17_Chr2g0296561 51.128 133 56 3 34 166 26 149 2.04e-32 119
MsaG007717 MtrunA17_Chr8g0377781 28.475 295 208 2 79 370 74 368 5.34e-31 121
MsaG007717 MtrunA17_Chr8g0377801 24.252 301 225 2 77 374 85 385 1.70e-24 103
MsaG007717 MtrunA17_Chr4g0067131 51.852 81 39 0 292 372 1 81 1.55e-22 90.9
MsaG007717 MtrunA17_Chr4g0067681 52.459 61 29 0 281 341 1 61 1.03e-15 71.2
MsaG007717 MtrunA17_Chr8g0339041 21.429 294 212 8 92 374 63 348 4.87e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007717 AT5G07900.1 33.634 333 213 4 47 373 69 399 6.73e-63 207
MsaG007717 AT1G21150.1 30.816 331 221 4 47 371 56 384 2.79e-46 163
MsaG007717 AT1G21150.2 30.816 331 221 4 47 371 120 448 1.04e-45 163
MsaG007717 AT1G21150.3 29.912 341 231 4 37 371 10 348 1.10e-45 160
MsaG007717 AT1G61990.2 28.493 365 213 6 48 373 53 408 7.26e-36 136
MsaG007717 AT1G61990.1 28.493 365 213 6 48 373 53 408 7.26e-36 136
MsaG007717 AT1G61980.3 28.841 371 213 9 47 373 52 415 9.22e-35 133
MsaG007717 AT1G61980.2 28.841 371 213 9 47 373 52 415 9.22e-35 133
MsaG007717 AT1G61980.1 28.841 371 213 9 47 373 52 415 9.22e-35 133
MsaG007717 AT1G61970.2 28.732 355 204 8 47 359 52 399 2.89e-34 131
MsaG007717 AT1G61970.7 28.732 355 204 8 47 359 52 399 2.89e-34 131
MsaG007717 AT1G61970.6 28.732 355 204 8 47 359 52 399 2.89e-34 131
MsaG007717 AT1G61970.3 28.732 355 204 8 47 359 52 399 2.89e-34 131
MsaG007717 AT1G61970.5 28.732 355 204 8 47 359 52 399 2.89e-34 131
MsaG007717 AT1G61970.4 28.732 355 204 8 47 359 52 399 2.89e-34 131
MsaG007717 AT1G61970.1 28.732 355 204 8 47 359 52 399 2.89e-34 131
MsaG007717 AT1G62120.1 28.852 357 203 8 47 359 56 405 1.14e-31 125
MsaG007717 AT1G62085.1 27.132 387 206 11 47 359 55 439 9.22e-30 120
MsaG007717 AT1G62085.2 27.132 387 206 11 47 359 55 439 9.22e-30 120
MsaG007717 AT3G46950.1 30.363 303 198 7 25 321 29 324 7.40e-29 117
MsaG007717 AT5G64950.1 26.225 347 234 6 34 365 27 366 7.17e-28 113
MsaG007717 AT1G61960.1 25.062 403 221 9 48 373 53 451 5.48e-26 109
MsaG007717 AT1G62010.1 26.349 315 193 4 82 359 71 383 1.83e-25 107
MsaG007717 AT5G23930.1 24.479 384 212 7 47 359 55 431 8.06e-25 105
MsaG007717 AT1G62110.1 28.521 284 189 6 47 321 52 330 4.40e-23 100
MsaG007717 AT1G62150.1 28.085 235 164 3 47 278 58 290 2.59e-19 89.7
MsaG007717 AT1G56380.1 23.706 367 216 10 45 359 14 368 6.86e-17 82.0
MsaG007717 AT1G56380.2 23.706 367 216 10 45 359 25 379 8.60e-17 81.6

Find 87 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGAATGATATGACGTAAT+TGG 0.199215 2:+22139179 None:intergenic
TCCTATCTCATTCACACTTT+TGG 0.219454 2:-22139316 MsaT007717.1:CDS
GTTGAAAAGGGTTAGGGTTT+GGG 0.231224 2:+22139404 None:intergenic
TTAATTTAGTGATGAACTTT+TGG 0.234643 2:-22138604 MsaT007717.1:CDS
ATTCAAAATTCACATCTCTT+TGG 0.274713 2:-22138944 MsaT007717.1:CDS
AGACCAACCCCATTTCTTAA+AGG 0.307244 2:+22138691 None:intergenic
AAACTTTGGCAACACCCTTT+TGG 0.315392 2:+22139126 None:intergenic
CTCAGTTTCTTTAGAAATCA+TGG 0.318502 2:-22139214 MsaT007717.1:CDS
TCCCTTGAGATGATAGATTA+GGG 0.318644 2:+22139444 None:intergenic
TGTTGAAAAGGGTTAGGGTT+TGG 0.321557 2:+22139403 None:intergenic
GTGCAGAAATGCAGAGAAAA+TGG 0.329555 2:+22139370 None:intergenic
CTTGGTTCTTGCTGGAAGCC+TGG 0.349228 2:-22139041 MsaT007717.1:CDS
GAGGAATGTTGAAAAGGGTT+AGG 0.357311 2:+22139397 None:intergenic
CAACTATAAACAAAACCTTA+TGG 0.357342 2:-22138730 MsaT007717.1:CDS
AAGAGCCCCTTGGTTCTTGC+TGG 0.357827 2:-22139049 MsaT007717.1:CDS
AGTCGATACCTTTAAGAAAT+GGG 0.363719 2:-22138699 MsaT007717.1:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2:-22138585 MsaT007717.1:CDS
GTACACACCAGGGACAAAAT+TGG 0.368393 2:-22138329 MsaT007717.1:CDS
AAGTCGATACCTTTAAGAAA+TGG 0.369211 2:-22138700 MsaT007717.1:CDS
GGGACTGGAGTCAGGTATTC+CGG 0.370799 2:-22138844 MsaT007717.1:CDS
GAGGTTGGTGGAGGAATTAA+AGG 0.376599 2:-22138807 MsaT007717.1:CDS
GGGTTAGGGTTTGGGGATTG+TGG 0.389338 2:+22139412 None:intergenic
TTTAGTCCCAACCTACGAAT+TGG 0.397525 2:-22139011 MsaT007717.1:CDS
GGCAATGAGGAATGTTGAAA+AGG 0.405575 2:+22139391 None:intergenic
GTGCAATATCTTCTAAAGAA+AGG 0.408077 2:-22138485 MsaT007717.1:CDS
ATCCCTTGAGATGATAGATT+AGG 0.409134 2:+22139443 None:intergenic
GAGAATGATATGACGTAATT+GGG 0.415442 2:+22139180 None:intergenic
GTCAGAAGTAGAAGCACCTT+TGG 0.415567 2:+22139090 None:intergenic
TTGAAAAGGGTTAGGGTTTG+GGG 0.438703 2:+22139405 None:intergenic
ATCGACTTTCTCTTTCCATA+AGG 0.444594 2:+22138715 None:intergenic
GCATTTCTGCACTAAAACTT+CGG 0.453039 2:-22139359 MsaT007717.1:CDS
TGGCAAGATTGCTTCGGGAC+TGG 0.453668 2:-22138859 MsaT007717.1:CDS
TGGAGGAATTAAAGGATTTG+AGG 0.454242 2:-22138799 MsaT007717.1:CDS
AACGTATTATGTATGTGTTA+AGG 0.454424 2:+22138405 None:intergenic
AGGAATGTTGAAAAGGGTTA+GGG 0.455795 2:+22139398 None:intergenic
AAGAAACTGAGAACAGAATC+AGG 0.455801 2:+22139226 None:intergenic
TATAAACCAATTCGTAGGTT+GGG 0.458485 2:+22139005 None:intergenic
ATTGCTTCGGGACTGGAGTC+AGG 0.459523 2:-22138852 MsaT007717.1:CDS
ACTAATAACCCTTGGTACAT+TGG 0.472256 2:+22138547 None:intergenic
CAAACATGGCAAGATTGCTT+CGG 0.473757 2:-22138865 MsaT007717.1:CDS
GCAATGAGGAATGTTGAAAA+GGG 0.479958 2:+22139392 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.480856 2:-22138586 MsaT007717.1:CDS
TTTCCTGCGACCCCTCCAAA+AGG 0.489524 2:-22139141 MsaT007717.1:CDS
GCCAAAAGTGTGAATGAGAT+AGG 0.495345 2:+22139315 None:intergenic
AAACATGGCAAGATTGCTTC+GGG 0.506298 2:-22138864 MsaT007717.1:CDS
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2:-22138576 MsaT007717.1:CDS
CTATAAACCAATTCGTAGGT+TGG 0.510197 2:+22139004 None:intergenic
AACCCTAATCTATCATCTCA+AGG 0.510708 2:-22139446 MsaT007717.1:CDS
ACCTCGGTATGTCACCTGTC+CGG 0.513896 2:+22138825 None:intergenic
AAGAGTTTAGAAGCAAATTG+TGG 0.524390 2:+22139286 None:intergenic
ATCAGTCATTACAAATGAAG+TGG 0.533113 2:+22138436 None:intergenic
ATCTTCTAAAGAAAGGTTTG+CGG 0.535884 2:-22138478 MsaT007717.1:CDS
GTTGAAACGAACATTGTAAG+AGG 0.539788 2:+22139261 None:intergenic
CCTTGTCAGTAAGAGCCCCT+TGG 0.547312 2:-22139059 MsaT007717.1:CDS
AGCTGAATCTTGTACACACC+AGG 0.549695 2:-22138340 MsaT007717.1:CDS
AGATAGGACACAACAAATGA+AGG 0.552393 2:+22139331 None:intergenic
TTTAATTCCTCCACCAACCT+CGG 0.560343 2:+22138809 None:intergenic
TTCCTGCGACCCCTCCAAAA+GGG 0.564822 2:-22139140 MsaT007717.1:CDS
TGGAGTCAGGTATTCCGGAC+AGG 0.568713 2:-22138839 MsaT007717.1:CDS
GTCGATACCTTTAAGAAATG+GGG 0.575626 2:-22138698 MsaT007717.1:CDS
AGGCTTCCAGCAAGAACCAA+GGG 0.579663 2:+22139043 None:intergenic
TGACATACCGAGGTTGGTGG+AGG 0.582066 2:-22138816 MsaT007717.1:CDS
AACCCTTGGTACATTGGCAA+TGG 0.582930 2:+22138553 None:intergenic
CGGAGTGACCGACTCAAACA+TGG 0.586400 2:-22138879 MsaT007717.1:CDS
CCAGAAAATTATCATACCTA+GGG 0.587562 2:-22138516 MsaT007717.1:CDS
GGCCATTGCCAATGTACCAA+GGG 0.590859 2:-22138555 MsaT007717.1:CDS
ACCCTAATCTATCATCTCAA+GGG 0.591554 2:-22139445 MsaT007717.1:CDS
AGACTACAACTAATAACCCT+TGG 0.592723 2:+22138539 None:intergenic
CAATCTTGCCATGTTTGAGT+CGG 0.596864 2:+22138871 None:intergenic
ATTACGTCATATCATTCTCA+AGG 0.599986 2:-22139176 MsaT007717.1:CDS
AGGTGACATACCGAGGTTGG+TGG 0.600565 2:-22138819 MsaT007717.1:CDS
GGGTCGCAGGAAAGTAGCCA+TGG 0.602735 2:+22139151 None:intergenic
CAGGCTTCCAGCAAGAACCA+AGG 0.603768 2:+22139042 None:intergenic
TCCAGAAAATTATCATACCT+AGG 0.606904 2:-22138517 MsaT007717.1:CDS
ATCAGATTGCTAATTGAGAA+CGG 0.608545 2:-22138899 MsaT007717.1:CDS
GACAGGTGACATACCGAGGT+TGG 0.612646 2:-22138822 MsaT007717.1:CDS
CCAAGGGGCTCTTACTGACA+AGG 0.613557 2:+22139059 None:intergenic
ATACCTTTAAGAAATGGGGT+TGG 0.619145 2:-22138694 MsaT007717.1:CDS
TATTGCACAACTGAGGCCCT+AGG 0.619185 2:+22138500 None:intergenic
GAATCTATAAACCAATTCGT+AGG 0.623076 2:+22139000 None:intergenic
TGGCCATTGCCAATGTACCA+AGG 0.632658 2:-22138556 MsaT007717.1:CDS
TAGAAGATATTGCACAACTG+AGG 0.636070 2:+22138493 None:intergenic
ATGCAGAGAAAATGGCAATG+AGG 0.647774 2:+22139378 None:intergenic
TCCGGACAGGTGACATACCG+AGG 0.668311 2:-22138826 MsaT007717.1:CDS
ATATCATTCTCAAGGCACCA+TGG 0.688804 2:-22139168 MsaT007717.1:CDS
GCTGAATCTTGTACACACCA+GGG 0.712860 2:-22138339 MsaT007717.1:CDS
GGCTTCCAGCAAGAACCAAG+GGG 0.719885 2:+22139044 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22138319 22139488 22138319 ID=MsaG007717
Chr2 mRNA 22138319 22139488 22138319 ID=MsaT007717.1;Parent=MsaG007717
Chr2 exon 22138319 22139488 22138319 ID=MsaT007717.1.exon1;Parent=MsaT007717.1
Chr2 CDS 22138319 22139488 22138319 ID=cds.MsaT007717.1;Parent=MsaT007717.1
Gene Sequence

>MsaT007717.1

ATGTTCAAATTCAATAGAAGAACCCTAATCTATCATCTCAAGGGATTAACTTCTCCACAATCCCCAAACCCTAACCCTTTTCAACATTCCTCATTGCCATTTTCTCTGCATTTCTGCACTAAAACTTCGGATTCACCTTCATTTGTTGTGTCCTATCTCATTCACACTTTTGGCTTTTCACCACAATTTGCTTCTAAACTCTTCTCCTCTTACAATGTTCGTTTCAACACCGCCCAAAAACCTGATTCTGTTCTCAGTTTCTTTAGAAATCATGGTTTCTCTGATTCCCAATTACGTCATATCATTCTCAAGGCACCATGGCTACTTTCCTGCGACCCCTCCAAAAGGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTACTTCTGACATTGTTAACCTTGTCAGTAAGAGCCCCTTGGTTCTTGCTGGAAGCCTGGAAAATCTTTTAGTCCCAACCTACGAATTGGTTTATAGATTCTTGCAATCTCACGATGACACTATTGCTTGTGTAATTCAAAATTCACATCTCTTTGGACAGAGTAATGTGTCATCTAATATCAGATTGCTAATTGAGAACGGAGTGACCGACTCAAACATGGCAAGATTGCTTCGGGACTGGAGTCAGGTATTCCGGACAGGTGACATACCGAGGTTGGTGGAGGAATTAAAGGATTTGAGGTTTAATCCTTCAAAAACATCTTTTGTTATAGCGCTGCTTGCCAAAACAACTATAAACAAAACCTTATGGAAAGAGAAAGTCGATACCTTTAAGAAATGGGGTTGGTCTGATGAAGATGTTGCTGAAGCATTTAGAAAAGACCCTCATTGTATGTTAAAATCCATTGATAAAATTAATTTAGTGATGAACTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCCAATGTACCAAGGGTTATTAGTTGTAGTCTCCAGAAAATTATCATACCTAGGGCCTCAGTTGTGCAATATCTTCTAAAGAAAGGTTTGCGGAAAAAGAATGCAAGCTTAACCACTTCATTTGTAATGACTGATAAGTCGTTCCTTAACACATACATAATACGTTTTAAGGAGGAGTCATCTTATCTCTTAAAGCTCTATGAAGAAAAGCTGAATCTTGTACACACCAGGGACAAAATTGGCATGTCATGA
Protein sequence

>MsaT007717.1

MFKFNRRTLIYHLKGLTSPQSPNPNPFQHSSLPFSLHFCTKTSDSPSFVVSYLIHTFGFSPQFASKLFSSYNVRFNTAQKPDSVLSFFRNHGFSDSQLRHIILKAPWLLSCDPSKRVLPKFQFFLSKGASTSDIVNLVSKSPLVLAGSLENLLVPTYELVYRFLQSHDDTIACVIQNSHLFGQSNVSSNIRLLIENGVTDSNMARLLRDWSQVFRTGDIPRLVEELKDLRFNPSKTSFVIALLAKTTINKTLWKEKVDTFKKWGWSDEDVAEAFRKDPHCMLKSIDKINLVMNFWVNQLGWDAMAIANVPRVISCSLQKIIIPRASVVQYLLKKGLRKKNASLTTSFVMTDKSFLNTYIIRFKEESSYLLKLYEEKLNLVHTRDKIGMS*