AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007718


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007718 MtrunA17_Chr2g0296111 88.710 372 39 1 23 394 15 383 0.0 661
MsaG007718 MtrunA17_Chr2g0296121 67.089 395 125 3 1 394 79 469 0.0 518
MsaG007718 MtrunA17_Chr2g0296151 66.076 395 119 4 1 393 1 382 0.0 515
MsaG007718 MtrunA17_Chr2g0295741 63.797 395 136 4 1 394 1 389 9.08e-172 484
MsaG007718 MtrunA17_Chr2g0295731 64.267 389 120 5 3 385 6 381 1.86e-170 481
MsaG007718 MtrunA17_Chr4g0067121 54.179 347 153 4 46 390 47 389 6.71e-124 363
MsaG007718 MtrunA17_Chr2g0296711 50.895 391 183 5 7 391 9 396 5.60e-123 362
MsaG007718 MtrunA17_Chr2g0296251 50.255 392 150 6 31 381 11 398 1.24e-122 360
MsaG007718 MtrunA17_Chr2g0296221 53.581 377 158 8 25 389 51 422 1.95e-122 361
MsaG007718 MtrunA17_Chr2g0296741 53.561 351 157 3 39 385 59 407 3.84e-122 360
MsaG007718 MtrunA17_Chr2g0296721 53.107 354 161 4 39 389 39 390 1.06e-120 355
MsaG007718 MtrunA17_Chr2g0315731 52.113 355 164 3 39 389 59 411 5.96e-118 349
MsaG007718 MtrunA17_Chr2g0296301 50.142 351 170 3 38 385 22 370 2.64e-117 346
MsaG007718 MtrunA17_Chr2g0296231 50.000 376 180 5 17 389 18 388 2.45e-116 344
MsaG007718 MtrunA17_Chr2g0296171 53.100 371 157 6 25 381 28 395 8.32e-116 343
MsaG007718 MtrunA17_Chr2g0296291 49.219 384 152 5 45 389 27 406 1.52e-114 340
MsaG007718 MtrunA17_Chr2g0296191 52.877 365 157 7 25 378 634 994 5.72e-110 346
MsaG007718 MtrunA17_Chr2g0296191 49.869 383 169 6 1 380 147 509 1.54e-107 339
MsaG007718 MtrunA17_Chr2g0296181 49.864 369 151 7 25 389 27 365 3.90e-107 320
MsaG007718 MtrunA17_Chr2g0295761 48.266 346 173 4 41 384 51 392 1.59e-105 316
MsaG007718 MtrunA17_Chr4g0067141 43.194 382 202 5 1 379 1 370 9.12e-94 286
MsaG007718 MtrunA17_Chr4g0067671 44.414 367 190 5 5 368 3 358 2.17e-89 274
MsaG007718 MtrunA17_Chr2g0296161 72.941 170 45 1 225 393 1 170 1.78e-85 257
MsaG007718 MtrunA17_Chr2g0286191 45.672 335 171 7 51 381 32 359 3.30e-83 258
MsaG007718 MtrunA17_Chr2g0296131 65.497 171 58 1 222 391 2 172 2.19e-73 226
MsaG007718 MtrunA17_Chr2g0295751 66.304 184 55 3 1 183 1 178 9.61e-71 230
MsaG007718 MtrunA17_Chr2g0296551 58.511 188 75 2 201 387 5 190 2.14e-68 214
MsaG007718 MtrunA17_Chr2g0296211 55.941 202 83 3 192 389 167 366 1.27e-66 216
MsaG007718 MtrunA17_Chr2g0296211 40.517 116 65 1 25 140 92 203 5.88e-20 90.5
MsaG007718 MtrunA17_Chr2g0296701 64.375 160 56 1 229 387 3 162 8.44e-66 207
MsaG007718 MtrunA17_Chr2g0295711 53.807 197 77 2 5 198 9 194 1.79e-60 194
MsaG007718 MtrunA17_Chr2g0296751 62.222 135 50 1 254 387 1 135 4.57e-52 171
MsaG007718 MtrunA17_Chr2g0295721 68.103 116 37 0 225 340 1 116 4.26e-51 167
MsaG007718 MtrunA17_Chr4g0001831 30.882 340 228 4 30 365 17 353 3.52e-45 159
MsaG007718 MtrunA17_Chr2g0286221 50.303 165 79 3 227 390 16 178 1.64e-43 149
MsaG007718 MtrunA17_Chr8g0377781 29.080 337 231 3 43 375 35 367 1.72e-34 131
MsaG007718 MtrunA17_Chr8g0377801 26.822 343 242 3 45 386 50 384 9.77e-34 129
MsaG007718 MtrunA17_Chr2g0296561 51.639 122 55 2 38 159 26 143 3.20e-30 113
MsaG007718 MtrunA17_Chr4g0067131 50.617 81 39 1 298 377 1 81 1.98e-18 79.7
MsaG007718 MtrunA17_Chr4g0067681 55.738 61 27 0 287 347 1 61 8.95e-17 74.3
MsaG007718 MtrunA17_Chr8g0339041 22.180 266 192 5 95 355 62 317 4.20e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007718 AT5G07900.1 34.309 376 229 8 17 378 28 399 2.78e-65 214
MsaG007718 AT1G21150.3 31.290 310 206 3 41 346 10 316 3.22e-44 157
MsaG007718 AT1G21150.1 32.333 300 196 3 51 346 56 352 7.49e-44 157
MsaG007718 AT1G21150.2 32.333 300 196 3 51 346 120 416 3.45e-43 156
MsaG007718 AT5G64950.1 28.155 309 204 5 41 337 26 328 2.06e-31 123
MsaG007718 AT1G61970.2 27.451 357 208 9 51 365 52 399 8.37e-29 116
MsaG007718 AT1G61970.7 27.451 357 208 9 51 365 52 399 8.37e-29 116
MsaG007718 AT1G61970.6 27.451 357 208 9 51 365 52 399 8.37e-29 116
MsaG007718 AT1G61970.3 27.451 357 208 9 51 365 52 399 8.37e-29 116
MsaG007718 AT1G61970.5 27.451 357 208 9 51 365 52 399 8.37e-29 116
MsaG007718 AT1G61970.4 27.451 357 208 9 51 365 52 399 8.37e-29 116
MsaG007718 AT1G61970.1 27.451 357 208 9 51 365 52 399 8.37e-29 116
MsaG007718 AT1G61980.3 28.492 358 205 9 51 366 52 400 9.33e-29 116
MsaG007718 AT1G61980.2 28.492 358 205 9 51 366 52 400 9.33e-29 116
MsaG007718 AT1G61980.1 28.492 358 205 9 51 366 52 400 9.33e-29 116
MsaG007718 AT1G61990.2 27.273 396 216 10 2 349 4 375 3.47e-28 115
MsaG007718 AT1G61990.1 27.273 396 216 10 2 349 4 375 3.47e-28 115
MsaG007718 AT1G62120.1 27.747 364 207 10 27 342 26 381 2.92e-27 112
MsaG007718 AT3G46950.1 30.112 269 172 7 34 297 34 291 1.38e-25 108
MsaG007718 AT1G62010.1 26.935 323 186 6 64 342 43 359 1.61e-22 99.0
MsaG007718 AT5G23930.1 25.355 422 226 11 23 363 20 433 4.63e-21 95.1
MsaG007718 AT1G62085.1 25.765 392 200 13 34 341 30 414 4.78e-21 95.1
MsaG007718 AT1G62085.2 25.765 392 200 13 34 341 30 414 4.78e-21 95.1
MsaG007718 AT1G61960.1 28.235 255 173 4 45 295 46 294 1.39e-19 90.5
MsaG007718 AT1G62110.1 26.374 273 189 5 28 295 30 295 6.17e-18 85.9
MsaG007718 AT1G62150.1 24.206 252 186 3 45 294 52 300 3.18e-16 80.5

Find 77 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTACCTCATCAACAATTT+TGG 0.159722 2:-22144736 MsaT007718.1:CDS
TTTCCTGCAACCCTTCTAAA+AGG 0.220666 2:-22144561 MsaT007718.1:CDS
TTAATTTAGTGATGAACTTT+TGG 0.234643 2:-22144018 MsaT007718.1:CDS
GAAATTGGTGGAGGAATTAA+AGG 0.238950 2:-22144221 MsaT007718.1:CDS
GGTGGAGGAATTAAAGGATT+TGG 0.242361 2:-22144215 MsaT007718.1:CDS
ATCAACTTCTTTAGAAACTA+TGG 0.267482 2:-22144634 MsaT007718.1:CDS
GTGGAGGAATTAAAGGATTT+GGG 0.314616 2:-22144214 MsaT007718.1:CDS
AGACCAACCCCACTTCTTAA+AGG 0.324708 2:+22144105 None:intergenic
GTTTCTTGATAAGTGTATAA+AGG 0.325915 2:-22143822 MsaT007718.1:CDS
TTAAAAGAATGCTTTGGTTA+AGG 0.353006 2:-22144279 MsaT007718.1:CDS
GACACATGACATGCTGAAAT+TGG 0.353711 2:-22144236 MsaT007718.1:CDS
TAGCCATGGTTCCTTTGAAA+TGG 0.395647 2:+22144585 None:intergenic
TTTCTTGATAAGTGTATAAA+GGG 0.396797 2:-22143821 MsaT007718.1:CDS
ATGTTCTACAAGCATTTAGA+AGG 0.400394 2:-22144075 MsaT007718.1:CDS
AGAGGGTTAGGGTTAGGGTT+TGG 0.404880 2:+22144841 None:intergenic
TGCGTATGTGGAGCAAAGTC+TGG 0.409089 2:+22144693 None:intergenic
TTGCAATATCTTCTGAAGAA+AGG 0.412458 2:-22143899 MsaT007718.1:CDS
TGAAATGGTAAGACGTAGTT+GGG 0.418042 2:+22144600 None:intergenic
TAAGTGTATAAAGGGTATTA+AGG 0.419835 2:-22143813 MsaT007718.1:CDS
GAATCTATAAATCAATTGAT+AGG 0.434987 2:+22144420 None:intergenic
ATTCGCAATCCATCTTTCTT+CGG 0.435209 2:-22144364 MsaT007718.1:CDS
CAAACATTAAAAGAATGCTT+TGG 0.436839 2:-22144285 MsaT007718.1:CDS
CTTAAAACTAATTGCGTATG+TGG 0.438575 2:+22144681 None:intergenic
TTATCTATTAAAGTTGTATG+AGG 0.446208 2:-22143783 MsaT007718.1:CDS
TTGGGTCAATCAGTTAGGTT+GGG 0.447898 2:-22143999 MsaT007718.1:CDS
TTTCTTTAAGTTTCGAAAAG+AGG 0.455763 2:-22143943 MsaT007718.1:CDS
AAGAAGTTGATAACAGAGTC+AGG 0.461266 2:+22144646 None:intergenic
TTGAAATGGTAAGACGTAGT+TGG 0.462482 2:+22144599 None:intergenic
TTTGGGTCAATCAGTTAGGT+TGG 0.464253 2:-22144000 MsaT007718.1:CDS
AATGCTTTGGTTAAGGAGTC+GGG 0.471203 2:-22144272 MsaT007718.1:CDS
GTCAGAGGTAGAAGCGCCTA+TGG 0.482625 2:+22144510 None:intergenic
TTCCTGCAACCCTTCTAAAA+GGG 0.482738 2:-22144560 MsaT007718.1:CDS
GTTTGAGTCGGCCACTCCAT+TGG 0.486888 2:+22144303 None:intergenic
GAATGCTTTGGTTAAGGAGT+CGG 0.487572 2:-22144273 MsaT007718.1:CDS
ATCTGTAAGGAAAACATTGT+GGG 0.488825 2:-22144143 MsaT007718.1:CDS
TTCTTTAAGTTTCGAAAAGA+GGG 0.495393 2:-22143942 MsaT007718.1:CDS
GAGAAAATGTTGGTGATGAT+AGG 0.497803 2:+22144803 None:intergenic
AAAGTCTGGAAGCAGATTGT+GGG 0.509506 2:+22144707 None:intergenic
AGGGTTTGGTGAGAAAGTTA+AGG 0.510973 2:+22144855 None:intergenic
CGAGCGTCTCCGAAGAAAGA+TGG 0.514763 2:+22144355 None:intergenic
TGCATGCCAAACTATCTGTA+AGG 0.519763 2:-22144156 MsaT007718.1:CDS
CAAAGTCTGGAAGCAGATTG+TGG 0.519859 2:+22144706 None:intergenic
TCGAAAAGAGGGTCCTTCCA+AGG 0.522254 2:-22143931 MsaT007718.1:CDS
GGTTGAATCTGAAGAGTTGG+TGG 0.537307 2:+22144771 None:intergenic
ACTACGTCTTACCATTTCAA+AGG 0.541064 2:-22144596 MsaT007718.1:CDS
ATTAGATTGCTGATTGCCAA+TGG 0.546889 2:-22144319 MsaT007718.1:CDS
AAACTGATGGTCCTTTGGCA+AGG 0.547751 2:+22143966 None:intergenic
AGAGGTTGAATCTGAAGAGT+TGG 0.550770 2:+22144768 None:intergenic
GAGGTAGGAGACTGCAAAAG+AGG 0.555968 2:+22144750 None:intergenic
AGTCGATGCCTTTAAGAAGT+GGG 0.559365 2:-22144113 MsaT007718.1:CDS
GATGCACTTGCCCTTGCCAA+AGG 0.563336 2:-22143977 MsaT007718.1:CDS
TATTGCAATACAGAGGCCCT+TGG 0.565124 2:+22143914 None:intergenic
AACTGATGGTCCTTTGGCAA+GGG 0.571808 2:+22143967 None:intergenic
TAGATTCTTGCAATCAGACA+AGG 0.576536 2:-22144404 MsaT007718.1:CDS
CGAAAAGAGGGTCCTTCCAA+GGG 0.576545 2:-22143930 MsaT007718.1:CDS
AAGTCGATGCCTTTAAGAAG+TGG 0.577016 2:-22144114 MsaT007718.1:CDS
TATCTGTAAGGAAAACATTG+TGG 0.577111 2:-22144144 MsaT007718.1:CDS
GCCAAAATTGTTGATGAGGT+AGG 0.577466 2:+22144735 None:intergenic
AGAGAAAAGCTCTATGACGA+TGG 0.581395 2:+22144887 None:intergenic
AAGGCAACCACATTGTATGT+TGG 0.594814 2:-22144056 MsaT007718.1:CDS
GCAATACAGAGGCCCTTGGA+AGG 0.596897 2:+22143918 None:intergenic
AAAAGCCAAAATTGTTGATG+AGG 0.598384 2:+22144731 None:intergenic
ATGCCTTTAAGAAGTGGGGT+TGG 0.598873 2:-22144108 MsaT007718.1:CDS
GTCGATGCCTTTAAGAAGTG+GGG 0.602513 2:-22144112 MsaT007718.1:CDS
GGCATGCAATGCTACACCAA+AGG 0.603475 2:+22144171 None:intergenic
GAAAATGTTAACAATGTCAG+AGG 0.609432 2:+22144495 None:intergenic
ACATGACATGCTGAAATTGG+TGG 0.610610 2:-22144233 MsaT007718.1:CDS
TTTCTTCGGAGACGCTCGTG+TGG 0.619956 2:-22144350 MsaT007718.1:CDS
TGACATGCTGAAATTGGTGG+AGG 0.631062 2:-22144230 MsaT007718.1:CDS
ACTCAATCTTGCTTACACCA+TGG 0.635583 2:-22143756 MsaT007718.1:CDS
AATTTCAGCATGTCATGTGT+CGG 0.639746 2:+22144238 None:intergenic
GGGTTGCAGGAAAGTAGCCA+TGG 0.641042 2:+22144571 None:intergenic
TTACCATTTCAAAGGAACCA+TGG 0.651356 2:-22144588 MsaT007718.1:CDS
CAGAAGATATTGCAATACAG+AGG 0.669772 2:+22143907 None:intergenic
AAGTCTGGAAGCAGATTGTG+GGG 0.672466 2:+22144708 None:intergenic
ATTGCTGATTGCCAATGGAG+TGG 0.672936 2:-22144314 MsaT007718.1:CDS
ATGGATACCAACATACAATG+TGG 0.711327 2:+22144049 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22143736 22144920 22143736 ID=MsaG007718
Chr2 mRNA 22143736 22144920 22143736 ID=MsaT007718.1;Parent=MsaG007718
Chr2 exon 22143736 22144920 22143736 ID=MsaT007718.1.exon1;Parent=MsaT007718.1
Chr2 CDS 22143736 22144920 22143736 ID=cds.MsaT007718.1;Parent=MsaT007718.1
Gene Sequence

>MsaT007718.1

ATGTTGAATATCCATCGTCATAGAGCTTTTCTCTATCTCAAAGCCTTAACTTTCTCACCAAACCCTAACCCTAACCCTCTTTTTCAACATTATTACCTATCATCACCAACATTTTCTCTCCATTTTTCCACCAACTCTTCAGATTCAACCTCTTTTGCAGTCTCCTACCTCATCAACAATTTTGGCTTTTCCCCACAATCTGCTTCCAGACTTTGCTCCACATACGCAATTAGTTTTAAGACTGCCCAAAAACCTGACTCTGTTATCAACTTCTTTAGAAACTATGGTTTTTCAGATTCCCAACTACGTCTTACCATTTCAAAGGAACCATGGCTACTTTCCTGCAACCCTTCTAAAAGGGTTTTGCCAAAGTTTCAATTTTTTCTCTCCATAGGCGCTTCTACCTCTGACATTGTTAACATTTTCACTAAGAGCCCCTACATCCTGAAAACAAGCCTCAATAATAAAATTATCAAAGCCTATCAATTGATTTATAGATTCTTGCAATCAGACAAGGACATTATTGATTGTGTAATTCGCAATCCATCTTTCTTCGGAGACGCTCGTGTGGTATCTAACATTAGATTGCTGATTGCCAATGGAGTGGCCGACTCAAACATTAAAAGAATGCTTTGGTTAAGGAGTCGGGCATTTCAGACACCGACACATGACATGCTGAAATTGGTGGAGGAATTAAAGGATTTGGGTTTTGCTACTTCAAAAACAACCTTTGGTGTAGCATTGCATGCCAAACTATCTGTAAGGAAAACATTGTGGGAAGAGAAAGTCGATGCCTTTAAGAAGTGGGGTTGGTCTGATGAAGATGTTCTACAAGCATTTAGAAGGCAACCACATTGTATGTTGGTATCCATTGATAAAATTAATTTAGTGATGAACTTTTGGGTCAATCAGTTAGGTTGGGATGCACTTGCCCTTGCCAAAGGACCATCAGTTTTTTCTTTAAGTTTCGAAAAGAGGGTCCTTCCAAGGGCCTCTGTATTGCAATATCTTCTGAAGAAAGGTTTGCGAAAAAAGGATGCAAGCTTAACTTGTCCATTTGTAGTGCCTGAAAAGCTGTTTCTTGATAAGTGTATAAAGGGTATTAAGGAGTCTTCTTATCTATTAAAGTTGTATGAGGAAAAACTCAATCTTGCTTACACCATGGACAAAACATGCACATCGTGA

Protein sequence

>MsaT007718.1

MLNIHRHRAFLYLKALTFSPNPNPNPLFQHYYLSSPTFSLHFSTNSSDSTSFAVSYLINNFGFSPQSASRLCSTYAISFKTAQKPDSVINFFRNYGFSDSQLRLTISKEPWLLSCNPSKRVLPKFQFFLSIGASTSDIVNIFTKSPYILKTSLNNKIIKAYQLIYRFLQSDKDIIDCVIRNPSFFGDARVVSNIRLLIANGVADSNIKRMLWLRSRAFQTPTHDMLKLVEELKDLGFATSKTTFGVALHAKLSVRKTLWEEKVDAFKKWGWSDEDVLQAFRRQPHCMLVSIDKINLVMNFWVNQLGWDALALAKGPSVFSLSFEKRVLPRASVLQYLLKKGLRKKDASLTCPFVVPEKLFLDKCIKGIKESSYLLKLYEEKLNLAYTMDKTCTS*