AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007719


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007719 MtrunA17_Chr2g0295731 90.000 330 33 0 19 348 52 381 0.0 608
MsaG007719 MtrunA17_Chr2g0296151 70.997 331 96 0 18 348 44 374 3.09e-170 479
MsaG007719 MtrunA17_Chr2g0296121 69.697 330 100 0 19 348 131 460 1.35e-161 460
MsaG007719 MtrunA17_Chr2g0295741 66.566 332 111 0 17 348 49 380 2.00e-156 444
MsaG007719 MtrunA17_Chr2g0296111 66.667 333 108 2 18 348 43 374 5.77e-156 442
MsaG007719 MtrunA17_Chr4g0067121 51.964 331 156 2 19 348 56 384 2.21e-122 357
MsaG007719 MtrunA17_Chr2g0296221 52.976 336 153 3 18 348 83 418 4.02e-119 351
MsaG007719 MtrunA17_Chr2g0296711 52.096 334 157 2 18 348 57 390 4.71e-119 350
MsaG007719 MtrunA17_Chr2g0296741 50.893 336 158 3 18 348 74 407 5.55e-116 342
MsaG007719 MtrunA17_Chr2g0296171 53.313 332 150 3 18 344 64 395 1.46e-115 341
MsaG007719 MtrunA17_Chr2g0296721 49.249 333 167 1 18 348 54 386 1.02e-112 333
MsaG007719 MtrunA17_Chr2g0296231 51.064 329 156 2 19 344 54 380 7.74e-112 331
MsaG007719 MtrunA17_Chr2g0315731 49.263 339 159 2 18 348 74 407 9.93e-111 329
MsaG007719 MtrunA17_Chr2g0296251 46.575 365 155 3 18 344 36 398 1.09e-110 328
MsaG007719 MtrunA17_Chr2g0296191 52.280 329 152 3 18 341 666 994 4.87e-108 338
MsaG007719 MtrunA17_Chr2g0296191 50.307 326 160 1 18 343 186 509 2.20e-104 329
MsaG007719 MtrunA17_Chr2g0296301 46.341 328 172 2 18 343 40 365 4.86e-106 316
MsaG007719 MtrunA17_Chr2g0295761 47.866 328 168 2 18 344 64 389 1.06e-105 315
MsaG007719 MtrunA17_Chr2g0296291 44.444 369 165 3 18 348 36 402 2.00e-105 315
MsaG007719 MtrunA17_Chr2g0286191 47.130 331 169 4 18 346 35 361 6.06e-98 294
MsaG007719 MtrunA17_Chr2g0296181 47.273 330 144 3 18 344 55 357 2.43e-93 283
MsaG007719 MtrunA17_Chr4g0067141 42.769 325 184 1 18 342 48 370 9.64e-89 271
MsaG007719 MtrunA17_Chr4g0067671 42.765 311 176 1 19 329 49 357 8.80e-85 260
MsaG007719 MtrunA17_Chr2g0296161 69.136 162 50 0 187 348 1 162 7.88e-77 233
MsaG007719 MtrunA17_Chr2g0296131 67.089 158 52 0 191 348 9 166 6.51e-74 226
MsaG007719 MtrunA17_Chr2g0295711 76.552 145 34 0 19 163 51 195 1.58e-71 221
MsaG007719 MtrunA17_Chr2g0296551 54.891 184 83 0 165 348 5 188 1.25e-68 213
MsaG007719 MtrunA17_Chr2g0295721 87.069 116 15 0 187 302 1 116 1.37e-66 205
MsaG007719 MtrunA17_Chr2g0296211 54.082 196 87 1 156 348 167 362 6.35e-65 210
MsaG007719 MtrunA17_Chr2g0296701 57.862 159 67 0 190 348 2 160 2.25e-60 191
MsaG007719 MtrunA17_Chr2g0295751 68.800 125 39 0 19 143 52 176 1.44e-50 175
MsaG007719 MtrunA17_Chr4g0001831 31.737 334 215 5 21 346 44 372 1.77e-49 169
MsaG007719 MtrunA17_Chr2g0296751 62.400 125 47 0 220 344 5 129 2.93e-49 162
MsaG007719 MtrunA17_Chr2g0286221 51.235 162 73 3 189 348 16 173 6.95e-48 159
MsaG007719 MtrunA17_Chr8g0377781 29.831 295 204 2 47 338 74 368 1.35e-37 138
MsaG007719 MtrunA17_Chr8g0377801 26.807 332 235 4 18 342 55 385 2.49e-35 132
MsaG007719 MtrunA17_Chr2g0296561 46.552 116 55 2 19 134 41 149 4.06e-25 99.4
MsaG007719 MtrunA17_Chr4g0067131 51.852 81 39 0 260 340 1 81 1.37e-23 93.2
MsaG007719 MtrunA17_Chr4g0067681 54.098 61 28 0 249 309 1 61 6.82e-16 71.6
MsaG007719 MtrunA17_Chr8g0339041 19.932 296 232 4 56 347 59 353 2.20e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007719 AT5G07900.1 34.756 328 206 4 20 341 74 399 8.75e-66 213
MsaG007719 AT1G21150.3 31.065 338 217 6 18 345 23 354 6.09e-48 165
MsaG007719 AT1G21150.1 31.065 338 217 6 18 345 59 390 1.50e-47 165
MsaG007719 AT1G21150.2 31.065 338 217 6 18 345 123 454 4.71e-47 166
MsaG007719 AT1G61980.3 27.778 360 207 7 18 333 55 405 1.75e-37 139
MsaG007719 AT1G61980.2 27.778 360 207 7 18 333 55 405 1.75e-37 139
MsaG007719 AT1G61980.1 27.778 360 207 7 18 333 55 405 1.75e-37 139
MsaG007719 AT1G62120.1 26.050 357 219 7 18 333 59 411 6.34e-35 132
MsaG007719 AT1G61990.2 27.671 365 212 7 18 341 55 408 6.32e-34 129
MsaG007719 AT1G61990.1 27.671 365 212 7 18 341 55 408 6.32e-34 129
MsaG007719 AT1G61970.2 26.857 350 211 7 18 327 55 399 1.25e-33 129
MsaG007719 AT1G61970.7 26.857 350 211 7 18 327 55 399 1.25e-33 129
MsaG007719 AT1G61970.6 26.857 350 211 7 18 327 55 399 1.25e-33 129
MsaG007719 AT1G61970.3 26.857 350 211 7 18 327 55 399 1.25e-33 129
MsaG007719 AT1G61970.5 26.857 350 211 7 18 327 55 399 1.25e-33 129
MsaG007719 AT1G61970.4 26.857 350 211 7 18 327 55 399 1.25e-33 129
MsaG007719 AT1G61970.1 26.857 350 211 7 18 327 55 399 1.25e-33 129
MsaG007719 AT3G46950.1 26.250 400 217 10 18 345 55 448 2.97e-33 128
MsaG007719 AT1G62085.1 25.758 396 206 10 18 333 58 445 4.41e-32 125
MsaG007719 AT1G62085.2 25.758 396 206 10 18 333 58 445 4.41e-32 125
MsaG007719 AT5G64950.1 29.577 355 228 8 4 342 28 376 1.08e-31 123
MsaG007719 AT1G62010.1 25.857 321 199 5 50 333 71 389 3.14e-30 119
MsaG007719 AT1G61960.1 24.250 400 222 9 19 341 56 451 4.43e-27 111
MsaG007719 AT1G62110.1 28.916 249 168 4 18 261 55 299 9.75e-26 107
MsaG007719 AT1G62110.1 20.563 355 231 11 21 328 90 440 5.47e-11 63.9
MsaG007719 AT1G62150.1 22.829 403 221 10 18 340 61 453 6.50e-24 102
MsaG007719 AT5G23930.1 19.010 384 237 7 18 332 58 436 1.22e-13 72.0

Find 59 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTACCTCATCAACAATTT+TGG 0.089846 2:+22147084 MsaT007719.1:CDS
GCAATCTGACAAGGAAATTT+TGG 0.119034 2:+22147425 MsaT007719.1:CDS
CTCAAATTCTTTACAAACTA+TGG 0.179377 2:+22147186 MsaT007719.1:CDS
TAAATTTAGTGATGGACTTT+TGG 0.199642 2:+22147796 MsaT007719.1:CDS
GTGAAGGAATTAAAGGATTT+GGG 0.230293 2:+22147600 MsaT007719.1:CDS
CTTGTAAGTGGAACAAAATT+TGG 0.240532 2:-22147127 None:intergenic
AGACCAACCCCATTTCTTAA+AGG 0.251996 2:-22147709 None:intergenic
TGACAAAATATAAGGTGTTT+TGG 0.267880 2:-22147853 None:intergenic
GGTATTGATTACTGTTGTTT+TGG 0.274832 2:-22147664 None:intergenic
AAGAATTTGAGAACAGATTC+AGG 0.277439 2:-22147174 None:intergenic
GGTGAAGGAATTAAAGGATT+TGG 0.282534 2:+22147599 MsaT007719.1:CDS
ATGGATGTTAACATACAATA+AGG 0.292032 2:-22147765 None:intergenic
AGGTTGGGACTATTTGATTC+TGG 0.292547 2:-22147380 None:intergenic
AAACTTTGGCAACACCGTTT+TGG 0.327649 2:-22147274 None:intergenic
AAACTTAATGACAAAATATA+AGG 0.334003 2:-22147861 None:intergenic
AAGTTGATACCTTTAAGAAA+TGG 0.365182 2:+22147700 MsaT007719.1:CDS
AGTTGATACCTTTAAGAAAT+GGG 0.365271 2:+22147701 MsaT007719.1:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2:+22147815 MsaT007719.1:CDS
CAAGTCGGTGAAGGAATTAA+AGG 0.378802 2:+22147593 MsaT007719.1:CDS
AACTTATCAGTCACTACAAA+TGG 0.416436 2:-22147969 None:intergenic
GTCAGAGGTAGAAGCACCTT+TGG 0.417863 2:-22147310 None:intergenic
AATAGTCCCAACCTATGAAT+TGG 0.421560 2:+22147389 MsaT007719.1:CDS
TTTGGGTCAATCAGTTGGGT+TGG 0.431987 2:+22147814 MsaT007719.1:CDS
TGGCAATCATATCGCGTAGT+TGG 0.438979 2:-22147221 None:intergenic
TATCAACTTTCTCTTTCCAT+AGG 0.439630 2:-22147686 None:intergenic
GGTTTGCAGGAAAGCAGCTC+TGG 0.441213 2:-22147249 None:intergenic
GGCAATCATATCGCGTAGTT+GGG 0.453430 2:-22147220 None:intergenic
ATCACAATGCTGATTGAGAA+TGG 0.457142 2:+22147501 MsaT007719.1:CDS
GTTGAAACGAACCTTGTAAG+TGG 0.460213 2:-22147139 None:intergenic
GGAAAGCAGCTCTGGCATCT+TGG 0.471973 2:-22147241 None:intergenic
GAATCTGTACACCAATTCAT+AGG 0.478001 2:-22147400 None:intergenic
CTGTACACCAATTCATAGGT+TGG 0.484595 2:-22147396 None:intergenic
AGGCTTGCATACAAAACTCT+AGG 0.506576 2:-22147357 None:intergenic
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2:+22147824 MsaT007719.1:CDS
TGTACACCAATTCATAGGTT+GGG 0.507173 2:-22147395 None:intergenic
TCACAATGCTGATTGAGAAT+GGG 0.508211 2:+22147502 MsaT007719.1:CDS
CAACAGTAATCAATACCCTA+TGG 0.513109 2:+22147670 MsaT007719.1:CDS
ATCAACTTTCTCTTTCCATA+GGG 0.529759 2:-22147685 None:intergenic
GTGCAATTTCTTCTCAACAA+AGG 0.536369 2:+22147915 MsaT007719.1:CDS
TGTGTCCTAACTCACTCATC+CGG 0.538232 2:-22146669 None:intergenic
TTCCTGCAAACCCTCCAAAA+CGG 0.545667 2:+22147260 MsaT007719.1:CDS
GTTGATACCTTTAAGAAATG+GGG 0.553181 2:+22147702 MsaT007719.1:CDS
AAACATTAAAACTTTGCTGA+GGG 0.556810 2:+22147536 MsaT007719.1:CDS
TTGGGACTATTTGATTCTGG+AGG 0.560239 2:-22147377 None:intergenic
AATTGCACAACAGCGGCCCT+CGG 0.562081 2:-22147900 None:intergenic
CCCAAAATTGTTGATGAGGT+AGG 0.566478 2:-22147085 None:intergenic
AAAACCCAAAATTGTTGATG+AGG 0.567106 2:-22147089 None:intergenic
CAGATTCTTGCAATCTGACA+AGG 0.575407 2:+22147416 MsaT007719.1:CDS
GGCTTGCATACAAAACTCTA+GGG 0.581368 2:-22147356 None:intergenic
ATACCTTTAAGAAATGGGGT+TGG 0.587688 2:+22147706 MsaT007719.1:CDS
CAAACATTAAAACTTTGCTG+AGG 0.594344 2:+22147535 MsaT007719.1:CDS
TGAAGGAATTAAAGGATTTG+GGG 0.597102 2:+22147601 MsaT007719.1:CDS
TGACATGCTCAAGTCGGTGA+AGG 0.598383 2:+22147584 MsaT007719.1:CDS
GACAATGTTAACAATGTCAG+AGG 0.618058 2:-22147325 None:intergenic
AAATTTGGAAGCAGATTGTG+AGG 0.621731 2:-22147112 None:intergenic
AAAATTGTTGATGAGGTAGG+AGG 0.622972 2:-22147082 None:intergenic
CACAATGCTGATTGAGAATG+GGG 0.632348 2:+22147503 MsaT007719.1:CDS
GAGAAGAAATTGCACAACAG+CGG 0.635644 2:-22147907 None:intergenic
GAGACGTGACATGCTCAAGT+CGG 0.666473 2:+22147578 MsaT007719.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22146625 22148076 22146625 ID=MsaG007719
Chr2 mRNA 22146625 22148076 22146625 ID=MsaT007719.1;Parent=MsaG007719
Chr2 exon 22146625 22146676 22146625 ID=MsaT007719.1.exon1;Parent=MsaT007719.1
Chr2 CDS 22146625 22146676 22146625 ID=cds.MsaT007719.1;Parent=MsaT007719.1
Chr2 exon 22147082 22148076 22147082 ID=MsaT007719.1.exon2;Parent=MsaT007719.1
Chr2 CDS 22147082 22148076 22147082 ID=cds.MsaT007719.1;Parent=MsaT007719.1
Gene Sequence

>MsaT007719.1

ATGAGTCGAAGTTCAAACCTCAATTATGAACAATTTTTCAACATCCGGATGACCTCCTACCTCATCAACAATTTTGGGTTTTCCTCACAATCTGCTTCCAAATTTTGTTCCACTTACAAGGTTCGTTTCAACAATGCCCAAAAACCTGAATCTGTTCTCAAATTCTTTACAAACTATGGTTTCTCTGATTCCCAACTACGCGATATGATTGCCAAGATGCCAGAGCTGCTTTCCTGCAAACCCTCCAAAACGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTACCTCTGACATTGTTAACATTGTCTCTACAAACCCTAGAGTTTTGTATGCAAGCCTCCAGAATCAAATAGTCCCAACCTATGAATTGGTGTACAGATTCTTGCAATCTGACAAGGAAATTTTGGCAAAATTGAGTTACAATGCAAATTTACTTTGTGATCGTACTGTGTATAATAACATCACAATGCTGATTGAGAATGGGGTGTCTGACTCAAACATTAAAACTTTGCTGAGGGAACAGACTCGAATATTCAAGAGACGTGACATGCTCAAGTCGGTGAAGGAATTAAAGGATTTGGGGTTTAATCCTTCAAAAACAACTTTTGGTGCAGCATTGCATGCCAAAACAACAGTAATCAATACCCTATGGAAAGAGAAAGTTGATACCTTTAAGAAATGGGGTTGGTCTGAAGAAGATTCTCTTGAAGCATTTAGAAAGAAACCTTATTGTATGTTAACATCCATTGAAAAAATAAATTTAGTGATGGACTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCCAAAACACCTTATATTTTGTCATTAAGTTTGGAAAAAAGGATCATTCCGAGGGCCGCTGTTGTGCAATTTCTTCTCAACAAAGGTTTGACAGATAAGAATGCAAGCTTAACTTATCCATTTGTAGTGACTGATAAGTTGTTTCTTAATATGTTTATAAAACGTTTTGAGAATGAGGCTTCTTATCTTTTAAAGTTGTATGAGGAAAAACGCAATCTAGGATAG

Protein sequence

>MsaT007719.1

MSRSSNLNYEQFFNIRMTSYLINNFGFSSQSASKFCSTYKVRFNNAQKPESVLKFFTNYGFSDSQLRDMIAKMPELLSCKPSKTVLPKFQFFLSKGASTSDIVNIVSTNPRVLYASLQNQIVPTYELVYRFLQSDKEILAKLSYNANLLCDRTVYNNITMLIENGVSDSNIKTLLREQTRIFKRRDMLKSVKELKDLGFNPSKTTFGAALHAKTTVINTLWKEKVDTFKKWGWSEEDSLEAFRKKPYCMLTSIEKINLVMDFWVNQLGWDAMAIAKTPYILSLSLEKRIIPRAAVVQFLLNKGLTDKNASLTYPFVVTDKLFLNMFIKRFENEASYLLKLYEEKRNLG*