Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG007720 | KEH37235.1 | 96.212 | 132 | 5 | 0 | 1 | 132 | 248 | 379 | 4.83e-87 | 268 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG007720 | tr|A0A072VGE0|A0A072VGE0_MEDTR | 96.212 | 132 | 5 | 0 | 1 | 132 | 248 | 379 | 2.31e-87 | 268 |
Gene ID | Type | Classification |
---|---|---|
MsaG007720 | TR | mTERF |
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsaG000180 | MsaG007720 | 0.817368 | 5.949673e-52 | 1.221788e-49 |
MsaG000405 | MsaG007720 | 0.821999 | 5.272117e-53 | 1.222595e-50 |
MsaG001366 | MsaG007720 | 0.809771 | 2.742225e-50 | 4.657130e-48 |
MsaG004105 | MsaG007720 | 0.806525 | 1.336820e-49 | 2.100547e-47 |
MsaG004793 | MsaG007720 | 0.807121 | 1.001895e-49 | 1.596524e-47 |
MsaG005329 | MsaG007720 | 0.815839 | 1.304239e-51 | 2.575388e-49 |
MsaG005774 | MsaG007720 | 0.812634 | 6.608050e-51 | 1.203791e-48 |
MsaG007353 | MsaG007720 | 0.830036 | 6.618076e-55 | 1.917333e-52 |
MsaG007568 | MsaG007720 | 0.807552 | 8.125807e-50 | 1.308198e-47 |
MsaG007720 | MsaG008220 | 0.804115 | 4.252643e-49 | 6.316477e-47 |
MsaG007720 | MsaG008324 | 0.826692 | 4.202845e-54 | 1.108029e-51 |
MsaG007720 | MsaG010816 | 0.810802 | 1.646662e-50 | 2.867620e-48 |
MsaG007720 | MsaG011080 | 0.804976 | 2.817378e-49 | 4.268973e-47 |
MsaG007720 | MsaG012287 | 0.831306 | 3.244177e-55 | 9.747081e-53 |
MsaG007720 | MsaG016567 | 0.808023 | 6.459328e-50 | 1.051773e-47 |
MsaG007720 | MsaG017170 | 0.814250 | 2.926655e-51 | 5.551829e-49 |
MsaG007720 | MsaG018134 | 0.800776 | 2.057710e-48 | 2.831409e-46 |
MsaG007720 | MsaG018909 | 0.804452 | 3.620127e-49 | 5.418999e-47 |
MsaG007720 | MsaG020078 | 0.818283 | 3.706374e-52 | 7.792547e-50 |
MsaG007720 | MsaG020787 | 0.808901 | 4.203934e-50 | 6.991478e-48 |
MsaG007720 | MsaG021026 | 0.811193 | 1.356591e-50 | 2.385117e-48 |
MsaG007720 | MsaG021471 | 0.804634 | 3.319305e-49 | 4.989593e-47 |
MsaG007720 | MsaG021485 | 0.822107 | 4.976185e-53 | 1.157327e-50 |
MsaG007720 | MsaG023761 | 0.802390 | 9.641130e-49 | 1.376127e-46 |
MsaG007720 | MsaG023762 | 0.803199 | 6.572578e-49 | 9.557424e-47 |
MsaG007720 | MsaG025287 | 0.808833 | 4.346936e-50 | 7.217463e-48 |
MsaG007720 | MsaG027340 | 0.803792 | 4.960456e-49 | 7.312551e-47 |
MsaG007720 | MsaG028698 | 0.802745 | 8.149485e-49 | 1.172686e-46 |
MsaG007720 | MsaG028804 | 0.802608 | 8.695429e-49 | 1.247364e-46 |
MsaG007720 | MsaG029232 | 0.806903 | 1.113537e-49 | 1.765284e-47 |
MsaG007720 | MsaG029482 | 0.808903 | 4.201280e-50 | 6.987275e-48 |
MsaG007720 | MsaG029487 | 0.817119 | 6.764959e-52 | 1.380349e-49 |
MsaG007720 | MsaG032046 | 0.800524 | 2.315682e-48 | 3.168170e-46 |
MsaG007720 | MsaG032786 | 0.801381 | 1.550126e-48 | 2.162255e-46 |
MsaG007720 | MsaG034207 | 0.833046 | 1.210207e-55 | 3.825405e-53 |
MsaG007720 | MsaG035463 | 0.829435 | 9.253854e-55 | 2.635136e-52 |
MsaG007720 | MsaG035675 | 0.809943 | 2.518568e-50 | 4.295030e-48 |
MsaG007720 | MsaG036241 | 0.820394 | 1.230616e-52 | 2.735007e-50 |
MsaG007720 | MsaG036420 | 0.860770 | 3.225748e-63 | 2.583047e-60 |
MsaG007720 | MsaG042510 | 0.807771 | 7.304743e-50 | 1.182214e-47 |
MsaG007720 | MsaG042898 | 0.800180 | 2.718639e-48 | 3.690593e-46 |
MsaG007720 | MsaG002433 | 0.800257 | 2.622446e-48 | 3.566304e-46 |
MsaG007720 | MsaG008955 | 0.808311 | 5.611675e-50 | 9.200680e-48 |
MsaG007720 | MsaG011633 | 0.802805 | 7.921508e-49 | 1.141445e-46 |
MsaG007720 | MsaG019592 | 0.816467 | 9.460041e-52 | 1.898039e-49 |
MsaG007720 | MsaG026536 | 0.805958 | 1.757963e-49 | 2.725382e-47 |
MsaG007720 | MsaG032663 | 0.832192 | 1.966195e-55 | 6.062324e-53 |
MsaG007720 | MsaG034613 | 0.817275 | 6.243269e-52 | 1.279043e-49 |
MsaG007720 | MsaG033153 | 0.809208 | 3.617360e-50 | 6.060135e-48 |
MsaG007720 | MsaG033504 | 0.811452 | 1.192248e-50 | 2.109574e-48 |
MsaG007720 | MsaG034183 | 0.809301 | 3.455435e-50 | 5.801806e-48 |
MsaG007720 | MsaG036665 | 0.809888 | 2.587674e-50 | 4.407110e-48 |
MsaG007720 | MsaG036543 | 0.822460 | 4.125430e-53 | 9.685990e-51 |
MsaG007720 | MsaG042833 | 0.800439 | 2.408407e-48 | 3.288849e-46 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG007720 | MtrunA17_Chr2g0295761 | 96.212 | 132 | 5 | 0 | 1 | 132 | 261 | 392 | 7.23e-91 | 268 |
MsaG007720 | MtrunA17_Chr2g0296251 | 62.016 | 129 | 49 | 0 | 1 | 129 | 270 | 398 | 8.30e-50 | 163 |
MsaG007720 | MtrunA17_Chr2g0296131 | 56.818 | 132 | 57 | 0 | 1 | 132 | 34 | 165 | 1.53e-49 | 156 |
MsaG007720 | MtrunA17_Chr2g0296231 | 61.538 | 130 | 48 | 1 | 1 | 130 | 254 | 381 | 5.62e-49 | 160 |
MsaG007720 | MtrunA17_Chr2g0296701 | 61.538 | 130 | 50 | 0 | 1 | 130 | 28 | 157 | 1.79e-48 | 153 |
MsaG007720 | MtrunA17_Chr2g0296551 | 58.462 | 130 | 54 | 0 | 1 | 130 | 56 | 185 | 2.81e-48 | 153 |
MsaG007720 | MtrunA17_Chr2g0296221 | 58.462 | 130 | 54 | 0 | 1 | 130 | 286 | 415 | 9.50e-48 | 159 |
MsaG007720 | MtrunA17_Chr2g0296291 | 59.690 | 129 | 52 | 0 | 1 | 129 | 270 | 398 | 6.60e-47 | 156 |
MsaG007720 | MtrunA17_Chr2g0296751 | 58.462 | 130 | 54 | 0 | 1 | 130 | 1 | 130 | 1.18e-46 | 148 |
MsaG007720 | MtrunA17_Chr4g0067121 | 52.273 | 132 | 63 | 0 | 1 | 132 | 252 | 383 | 1.13e-45 | 152 |
MsaG007720 | MtrunA17_Chr2g0296191 | 57.812 | 128 | 54 | 0 | 1 | 128 | 382 | 509 | 2.69e-45 | 157 |
MsaG007720 | MtrunA17_Chr2g0296191 | 57.143 | 126 | 54 | 0 | 1 | 126 | 869 | 994 | 2.06e-44 | 154 |
MsaG007720 | MtrunA17_Chr2g0296721 | 57.692 | 130 | 55 | 0 | 1 | 130 | 254 | 383 | 3.38e-45 | 151 |
MsaG007720 | MtrunA17_Chr2g0296211 | 58.915 | 129 | 53 | 0 | 2 | 130 | 231 | 359 | 7.03e-45 | 150 |
MsaG007720 | MtrunA17_Chr2g0296301 | 53.846 | 130 | 60 | 0 | 1 | 130 | 238 | 367 | 8.02e-45 | 150 |
MsaG007720 | MtrunA17_Chr2g0296161 | 50.758 | 132 | 65 | 0 | 1 | 132 | 30 | 161 | 1.36e-44 | 143 |
MsaG007720 | MtrunA17_Chr2g0295741 | 54.545 | 132 | 60 | 0 | 1 | 132 | 248 | 379 | 2.39e-44 | 149 |
MsaG007720 | MtrunA17_Chr2g0296741 | 57.600 | 125 | 53 | 0 | 6 | 130 | 280 | 404 | 2.49e-44 | 149 |
MsaG007720 | MtrunA17_Chr2g0286191 | 56.250 | 128 | 55 | 1 | 2 | 129 | 233 | 359 | 4.52e-44 | 147 |
MsaG007720 | MtrunA17_Chr2g0296171 | 57.364 | 129 | 55 | 0 | 1 | 129 | 267 | 395 | 5.36e-44 | 148 |
MsaG007720 | MtrunA17_Chr2g0296711 | 58.140 | 129 | 54 | 0 | 1 | 129 | 258 | 386 | 1.33e-43 | 147 |
MsaG007720 | MtrunA17_Chr2g0315731 | 55.385 | 130 | 58 | 0 | 1 | 130 | 275 | 404 | 1.37e-43 | 147 |
MsaG007720 | MtrunA17_Chr2g0296121 | 50.000 | 132 | 66 | 0 | 1 | 132 | 328 | 459 | 3.11e-43 | 147 |
MsaG007720 | MtrunA17_Chr2g0295731 | 51.515 | 132 | 64 | 0 | 1 | 132 | 249 | 380 | 5.63e-43 | 145 |
MsaG007720 | MtrunA17_Chr2g0296151 | 52.672 | 131 | 62 | 0 | 2 | 132 | 243 | 373 | 1.16e-42 | 144 |
MsaG007720 | MtrunA17_Chr2g0296111 | 54.615 | 130 | 58 | 1 | 3 | 132 | 245 | 373 | 2.26e-42 | 143 |
MsaG007720 | MtrunA17_Chr2g0286221 | 50.394 | 127 | 62 | 1 | 6 | 132 | 47 | 172 | 2.32e-37 | 125 |
MsaG007720 | MtrunA17_Chr4g0067141 | 46.825 | 126 | 67 | 0 | 1 | 126 | 244 | 369 | 5.66e-35 | 124 |
MsaG007720 | MtrunA17_Chr4g0067671 | 48.673 | 113 | 58 | 0 | 1 | 113 | 244 | 356 | 4.52e-34 | 121 |
MsaG007720 | MtrunA17_Chr2g0295721 | 57.143 | 84 | 36 | 0 | 1 | 84 | 30 | 113 | 2.26e-28 | 100 |
MsaG007720 | MtrunA17_Chr2g0296181 | 46.512 | 129 | 43 | 1 | 2 | 130 | 256 | 358 | 2.78e-26 | 101 |
MsaG007720 | MtrunA17_Chr4g0067131 | 50.617 | 81 | 40 | 0 | 45 | 125 | 1 | 81 | 7.78e-25 | 90.5 |
MsaG007720 | MtrunA17_Chr4g0001831 | 34.127 | 126 | 83 | 0 | 1 | 126 | 242 | 367 | 2.57e-19 | 82.0 |
MsaG007720 | MtrunA17_Chr4g0067681 | 46.774 | 62 | 33 | 0 | 34 | 95 | 1 | 62 | 5.71e-17 | 70.1 |
MsaG007720 | MtrunA17_Chr8g0377781 | 30.894 | 123 | 85 | 0 | 1 | 123 | 246 | 368 | 7.75e-13 | 63.9 |
MsaG007720 | MtrunA17_Chr8g0377801 | 26.984 | 126 | 92 | 0 | 1 | 126 | 259 | 384 | 1.04e-11 | 60.8 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsaG007720 | AT5G07900.1 | 40.476 | 126 | 75 | 0 | 2 | 127 | 275 | 400 | 6.23e-27 | 103 |
MsaG007720 | AT1G21150.3 | 33.333 | 126 | 83 | 1 | 2 | 126 | 225 | 350 | 7.96e-17 | 75.5 |
MsaG007720 | AT1G21150.1 | 33.333 | 126 | 83 | 1 | 2 | 126 | 261 | 386 | 1.00e-16 | 75.5 |
MsaG007720 | AT1G21150.2 | 33.333 | 126 | 83 | 1 | 2 | 126 | 325 | 450 | 1.07e-16 | 75.5 |
MsaG007720 | AT1G61990.2 | 33.051 | 118 | 76 | 1 | 12 | 126 | 291 | 408 | 5.55e-15 | 70.5 |
MsaG007720 | AT1G61990.1 | 33.051 | 118 | 76 | 1 | 12 | 126 | 291 | 408 | 5.55e-15 | 70.5 |
MsaG007720 | AT1G61970.2 | 34.314 | 102 | 66 | 1 | 12 | 112 | 298 | 399 | 1.40e-13 | 66.6 |
MsaG007720 | AT1G61970.7 | 34.314 | 102 | 66 | 1 | 12 | 112 | 298 | 399 | 1.40e-13 | 66.6 |
MsaG007720 | AT1G61970.6 | 34.314 | 102 | 66 | 1 | 12 | 112 | 298 | 399 | 1.40e-13 | 66.6 |
MsaG007720 | AT1G61970.3 | 34.314 | 102 | 66 | 1 | 12 | 112 | 298 | 399 | 1.40e-13 | 66.6 |
MsaG007720 | AT1G61970.5 | 34.314 | 102 | 66 | 1 | 12 | 112 | 298 | 399 | 1.40e-13 | 66.6 |
MsaG007720 | AT1G61970.4 | 34.314 | 102 | 66 | 1 | 12 | 112 | 298 | 399 | 1.40e-13 | 66.6 |
MsaG007720 | AT1G61970.1 | 34.314 | 102 | 66 | 1 | 12 | 112 | 298 | 399 | 1.40e-13 | 66.6 |
MsaG007720 | AT1G62010.1 | 34.314 | 102 | 66 | 1 | 12 | 112 | 282 | 383 | 1.60e-13 | 66.2 |
MsaG007720 | AT1G61960.1 | 30.081 | 123 | 83 | 1 | 12 | 131 | 334 | 456 | 1.85e-13 | 66.2 |
MsaG007720 | AT1G62120.1 | 33.628 | 113 | 74 | 1 | 2 | 113 | 294 | 406 | 6.82e-13 | 64.7 |
MsaG007720 | AT1G61980.3 | 33.051 | 118 | 76 | 2 | 12 | 126 | 298 | 415 | 1.86e-12 | 63.2 |
MsaG007720 | AT1G61980.2 | 33.051 | 118 | 76 | 2 | 12 | 126 | 298 | 415 | 1.86e-12 | 63.2 |
MsaG007720 | AT1G61980.1 | 33.051 | 118 | 76 | 2 | 12 | 126 | 298 | 415 | 1.86e-12 | 63.2 |
MsaG007720 | AT5G64950.1 | 32.824 | 131 | 83 | 2 | 1 | 126 | 245 | 375 | 8.49e-12 | 61.2 |
MsaG007720 | AT1G62085.1 | 32.039 | 103 | 69 | 1 | 12 | 113 | 338 | 440 | 7.55e-11 | 58.9 |
MsaG007720 | AT1G62085.2 | 32.039 | 103 | 69 | 1 | 12 | 113 | 338 | 440 | 7.55e-11 | 58.9 |
Find 27 sgRNAs with CRISPR-Local
Find sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TTAATAAGGTGATGAGATTT+TGG | 0.153456 | 2:-22151331 | MsaT007720.1:CDS |
TTTCGTATAGTTTGGAGAAT+AGG | 0.328948 | 2:-22151256 | MsaT007720.1:CDS |
ATTGAATTGTCTACTCCATT+TGG | 0.352685 | 2:-22151173 | MsaT007720.1:CDS |
TTCGTATAGTTTGGAGAATA+GGG | 0.359509 | 2:-22151255 | MsaT007720.1:CDS |
AAGTTGTTGTCTTTAAGAAA+TGG | 0.363837 | 2:-22151427 | MsaT007720.1:CDS |
ATCCACGGAGAAAATTAATA+AGG | 0.367860 | 2:-22151345 | MsaT007720.1:CDS |
TTGGGTCAATGAGTTGGGTT+GGG | 0.371128 | 2:-22151312 | MsaT007720.1:CDS |
AGTTGTTGTCTTTAAGAAAT+GGG | 0.373623 | 2:-22151426 | MsaT007720.1:CDS |
AAATGGTTCTTGAAGCGTTT+AGG | 0.395855 | 2:-22151391 | MsaT007720.1:CDS |
TGAGTTGGGTTGGGACTCTT+CGG | 0.410843 | 2:-22151303 | MsaT007720.1:CDS |
ACTCTTCGGCTCTTGTCAAA+AGG | 0.469912 | 2:-22151289 | MsaT007720.1:CDS |
TTCTTGAAGCGTTTAGGAAG+CGG | 0.487125 | 2:-22151385 | MsaT007720.1:CDS |
TTTGGGTCAATGAGTTGGGT+TGG | 0.489140 | 2:-22151313 | MsaT007720.1:CDS |
GGCCATGTCGAAATCACGTT+GGG | 0.495859 | 2:-22151456 | None:intergenic |
ATGGGGTTGGTCTGATGAAA+TGG | 0.499033 | 2:-22151408 | MsaT007720.1:CDS |
GTTGTTGTCTTTAAGAAATG+GGG | 0.513781 | 2:-22151425 | MsaT007720.1:CDS |
TGCGAAACAACACAAGCCCT+TGG | 0.528307 | 2:+22151227 | None:intergenic |
TTCGGCTCTTGTCAAAAGGC+CGG | 0.528513 | 2:-22151285 | MsaT007720.1:CDS |
TGGAGAATAGGGTCATTCCA+AGG | 0.540810 | 2:-22151244 | MsaT007720.1:CDS |
TTGTCTTTAAGAAATGGGGT+TGG | 0.562623 | 2:-22151421 | MsaT007720.1:CDS |
ATTGTGTATGTTAGCATCCA+CGG | 0.574891 | 2:-22151360 | MsaT007720.1:CDS |
GGAGAATAGGGTCATTCCAA+GGG | 0.578298 | 2:-22151243 | MsaT007720.1:CDS |
GGCTCTTGTCAAAAGGCCGG+AGG | 0.586864 | 2:-22151282 | MsaT007720.1:CDS |
GTTTCGCATTTGATCTCCAG+AGG | 0.592319 | 2:-22151212 | MsaT007720.1:CDS |
GGGCCATGTCGAAATCACGT+TGG | 0.650039 | 2:-22151457 | None:intergenic |
ATCCCAACGTGATTTCGACA+TGG | 0.666340 | 2:+22151454 | None:intergenic |
GTGGATGCTAACATACACAA+TGG | 0.700210 | 2:+22151362 | None:intergenic |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr2 | gene | 22151076 | 22151474 | 22151076 | ID=MsaG007720 |
Chr2 | mRNA | 22151076 | 22151474 | 22151076 | ID=MsaT007720.1;Parent=MsaG007720 |
Chr2 | exon | 22151076 | 22151474 | 22151076 | ID=MsaT007720.1.exon1;Parent=MsaT007720.1 |
Chr2 | CDS | 22151076 | 22151474 | 22151076 | ID=cds.MsaT007720.1;Parent=MsaT007720.1 |
Gene Sequence |
Protein sequence |