AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007720


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007720 MtrunA17_Chr2g0295761 96.212 132 5 0 1 132 261 392 7.23e-91 268
MsaG007720 MtrunA17_Chr2g0296251 62.016 129 49 0 1 129 270 398 8.30e-50 163
MsaG007720 MtrunA17_Chr2g0296131 56.818 132 57 0 1 132 34 165 1.53e-49 156
MsaG007720 MtrunA17_Chr2g0296231 61.538 130 48 1 1 130 254 381 5.62e-49 160
MsaG007720 MtrunA17_Chr2g0296701 61.538 130 50 0 1 130 28 157 1.79e-48 153
MsaG007720 MtrunA17_Chr2g0296551 58.462 130 54 0 1 130 56 185 2.81e-48 153
MsaG007720 MtrunA17_Chr2g0296221 58.462 130 54 0 1 130 286 415 9.50e-48 159
MsaG007720 MtrunA17_Chr2g0296291 59.690 129 52 0 1 129 270 398 6.60e-47 156
MsaG007720 MtrunA17_Chr2g0296751 58.462 130 54 0 1 130 1 130 1.18e-46 148
MsaG007720 MtrunA17_Chr4g0067121 52.273 132 63 0 1 132 252 383 1.13e-45 152
MsaG007720 MtrunA17_Chr2g0296191 57.812 128 54 0 1 128 382 509 2.69e-45 157
MsaG007720 MtrunA17_Chr2g0296191 57.143 126 54 0 1 126 869 994 2.06e-44 154
MsaG007720 MtrunA17_Chr2g0296721 57.692 130 55 0 1 130 254 383 3.38e-45 151
MsaG007720 MtrunA17_Chr2g0296211 58.915 129 53 0 2 130 231 359 7.03e-45 150
MsaG007720 MtrunA17_Chr2g0296301 53.846 130 60 0 1 130 238 367 8.02e-45 150
MsaG007720 MtrunA17_Chr2g0296161 50.758 132 65 0 1 132 30 161 1.36e-44 143
MsaG007720 MtrunA17_Chr2g0295741 54.545 132 60 0 1 132 248 379 2.39e-44 149
MsaG007720 MtrunA17_Chr2g0296741 57.600 125 53 0 6 130 280 404 2.49e-44 149
MsaG007720 MtrunA17_Chr2g0286191 56.250 128 55 1 2 129 233 359 4.52e-44 147
MsaG007720 MtrunA17_Chr2g0296171 57.364 129 55 0 1 129 267 395 5.36e-44 148
MsaG007720 MtrunA17_Chr2g0296711 58.140 129 54 0 1 129 258 386 1.33e-43 147
MsaG007720 MtrunA17_Chr2g0315731 55.385 130 58 0 1 130 275 404 1.37e-43 147
MsaG007720 MtrunA17_Chr2g0296121 50.000 132 66 0 1 132 328 459 3.11e-43 147
MsaG007720 MtrunA17_Chr2g0295731 51.515 132 64 0 1 132 249 380 5.63e-43 145
MsaG007720 MtrunA17_Chr2g0296151 52.672 131 62 0 2 132 243 373 1.16e-42 144
MsaG007720 MtrunA17_Chr2g0296111 54.615 130 58 1 3 132 245 373 2.26e-42 143
MsaG007720 MtrunA17_Chr2g0286221 50.394 127 62 1 6 132 47 172 2.32e-37 125
MsaG007720 MtrunA17_Chr4g0067141 46.825 126 67 0 1 126 244 369 5.66e-35 124
MsaG007720 MtrunA17_Chr4g0067671 48.673 113 58 0 1 113 244 356 4.52e-34 121
MsaG007720 MtrunA17_Chr2g0295721 57.143 84 36 0 1 84 30 113 2.26e-28 100
MsaG007720 MtrunA17_Chr2g0296181 46.512 129 43 1 2 130 256 358 2.78e-26 101
MsaG007720 MtrunA17_Chr4g0067131 50.617 81 40 0 45 125 1 81 7.78e-25 90.5
MsaG007720 MtrunA17_Chr4g0001831 34.127 126 83 0 1 126 242 367 2.57e-19 82.0
MsaG007720 MtrunA17_Chr4g0067681 46.774 62 33 0 34 95 1 62 5.71e-17 70.1
MsaG007720 MtrunA17_Chr8g0377781 30.894 123 85 0 1 123 246 368 7.75e-13 63.9
MsaG007720 MtrunA17_Chr8g0377801 26.984 126 92 0 1 126 259 384 1.04e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007720 AT5G07900.1 40.476 126 75 0 2 127 275 400 6.23e-27 103
MsaG007720 AT1G21150.3 33.333 126 83 1 2 126 225 350 7.96e-17 75.5
MsaG007720 AT1G21150.1 33.333 126 83 1 2 126 261 386 1.00e-16 75.5
MsaG007720 AT1G21150.2 33.333 126 83 1 2 126 325 450 1.07e-16 75.5
MsaG007720 AT1G61990.2 33.051 118 76 1 12 126 291 408 5.55e-15 70.5
MsaG007720 AT1G61990.1 33.051 118 76 1 12 126 291 408 5.55e-15 70.5
MsaG007720 AT1G61970.2 34.314 102 66 1 12 112 298 399 1.40e-13 66.6
MsaG007720 AT1G61970.7 34.314 102 66 1 12 112 298 399 1.40e-13 66.6
MsaG007720 AT1G61970.6 34.314 102 66 1 12 112 298 399 1.40e-13 66.6
MsaG007720 AT1G61970.3 34.314 102 66 1 12 112 298 399 1.40e-13 66.6
MsaG007720 AT1G61970.5 34.314 102 66 1 12 112 298 399 1.40e-13 66.6
MsaG007720 AT1G61970.4 34.314 102 66 1 12 112 298 399 1.40e-13 66.6
MsaG007720 AT1G61970.1 34.314 102 66 1 12 112 298 399 1.40e-13 66.6
MsaG007720 AT1G62010.1 34.314 102 66 1 12 112 282 383 1.60e-13 66.2
MsaG007720 AT1G61960.1 30.081 123 83 1 12 131 334 456 1.85e-13 66.2
MsaG007720 AT1G62120.1 33.628 113 74 1 2 113 294 406 6.82e-13 64.7
MsaG007720 AT1G61980.3 33.051 118 76 2 12 126 298 415 1.86e-12 63.2
MsaG007720 AT1G61980.2 33.051 118 76 2 12 126 298 415 1.86e-12 63.2
MsaG007720 AT1G61980.1 33.051 118 76 2 12 126 298 415 1.86e-12 63.2
MsaG007720 AT5G64950.1 32.824 131 83 2 1 126 245 375 8.49e-12 61.2
MsaG007720 AT1G62085.1 32.039 103 69 1 12 113 338 440 7.55e-11 58.9
MsaG007720 AT1G62085.2 32.039 103 69 1 12 113 338 440 7.55e-11 58.9

Find 27 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATAAGGTGATGAGATTT+TGG 0.153456 2:-22151331 MsaT007720.1:CDS
TTTCGTATAGTTTGGAGAAT+AGG 0.328948 2:-22151256 MsaT007720.1:CDS
ATTGAATTGTCTACTCCATT+TGG 0.352685 2:-22151173 MsaT007720.1:CDS
TTCGTATAGTTTGGAGAATA+GGG 0.359509 2:-22151255 MsaT007720.1:CDS
AAGTTGTTGTCTTTAAGAAA+TGG 0.363837 2:-22151427 MsaT007720.1:CDS
ATCCACGGAGAAAATTAATA+AGG 0.367860 2:-22151345 MsaT007720.1:CDS
TTGGGTCAATGAGTTGGGTT+GGG 0.371128 2:-22151312 MsaT007720.1:CDS
AGTTGTTGTCTTTAAGAAAT+GGG 0.373623 2:-22151426 MsaT007720.1:CDS
AAATGGTTCTTGAAGCGTTT+AGG 0.395855 2:-22151391 MsaT007720.1:CDS
TGAGTTGGGTTGGGACTCTT+CGG 0.410843 2:-22151303 MsaT007720.1:CDS
ACTCTTCGGCTCTTGTCAAA+AGG 0.469912 2:-22151289 MsaT007720.1:CDS
TTCTTGAAGCGTTTAGGAAG+CGG 0.487125 2:-22151385 MsaT007720.1:CDS
TTTGGGTCAATGAGTTGGGT+TGG 0.489140 2:-22151313 MsaT007720.1:CDS
GGCCATGTCGAAATCACGTT+GGG 0.495859 2:-22151456 None:intergenic
ATGGGGTTGGTCTGATGAAA+TGG 0.499033 2:-22151408 MsaT007720.1:CDS
GTTGTTGTCTTTAAGAAATG+GGG 0.513781 2:-22151425 MsaT007720.1:CDS
TGCGAAACAACACAAGCCCT+TGG 0.528307 2:+22151227 None:intergenic
TTCGGCTCTTGTCAAAAGGC+CGG 0.528513 2:-22151285 MsaT007720.1:CDS
TGGAGAATAGGGTCATTCCA+AGG 0.540810 2:-22151244 MsaT007720.1:CDS
TTGTCTTTAAGAAATGGGGT+TGG 0.562623 2:-22151421 MsaT007720.1:CDS
ATTGTGTATGTTAGCATCCA+CGG 0.574891 2:-22151360 MsaT007720.1:CDS
GGAGAATAGGGTCATTCCAA+GGG 0.578298 2:-22151243 MsaT007720.1:CDS
GGCTCTTGTCAAAAGGCCGG+AGG 0.586864 2:-22151282 MsaT007720.1:CDS
GTTTCGCATTTGATCTCCAG+AGG 0.592319 2:-22151212 MsaT007720.1:CDS
GGGCCATGTCGAAATCACGT+TGG 0.650039 2:-22151457 None:intergenic
ATCCCAACGTGATTTCGACA+TGG 0.666340 2:+22151454 None:intergenic
GTGGATGCTAACATACACAA+TGG 0.700210 2:+22151362 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22151076 22151474 22151076 ID=MsaG007720
Chr2 mRNA 22151076 22151474 22151076 ID=MsaT007720.1;Parent=MsaG007720
Chr2 exon 22151076 22151474 22151076 ID=MsaT007720.1.exon1;Parent=MsaT007720.1
Chr2 CDS 22151076 22151474 22151076 ID=cds.MsaT007720.1;Parent=MsaT007720.1
Gene Sequence

>MsaT007720.1

ATGTCGAAATCACGTTGGGATGCGAAAGTTGTTGTCTTTAAGAAATGGGGTTGGTCTGATGAAATGGTTCTTGAAGCGTTTAGGAAGCGGCCATTGTGTATGTTAGCATCCACGGAGAAAATTAATAAGGTGATGAGATTTTGGGTCAATGAGTTGGGTTGGGACTCTTCGGCTCTTGTCAAAAGGCCGGAGGTTTTTTCGTATAGTTTGGAGAATAGGGTCATTCCAAGGGCTTGTGTTGTTTCGCATTTGATCTCCAGAGGTTTGATAGAAAAGAATATTGAATTGTCTACTCCATTTGGTGTTAATGAAAATGTGTTTCTTGAAAAGTATGTGCAGTGTTTTAAGGAGGAAAGAGATGATTTGTTAAAGCTATACCTAGAAAAAATGGGTGTCTGA

Protein sequence

>MsaT007720.1

MSKSRWDAKVVVFKKWGWSDEMVLEAFRKRPLCMLASTEKINKVMRFWVNELGWDSSALVKRPEVFSYSLENRVIPRACVVSHLISRGLIEKNIELSTPFGVNENVFLEKYVQCFKEERDDLLKLYLEKMGV*