AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007746

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007746 MtrunA17_Chr8g0353751 63.504 137 50 0 90 226 744 880 3.29e-53 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007746 AT3G12280.2 57.463 134 53 2 90 223 735 864 4.08e-42 152
MsaG007746 AT3G12280.1 57.463 134 53 2 90 223 735 864 4.08e-42 152

Find 43 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTGTAGAGCCACTGCTTT+TGG 0.167941 2:-22481107 MsaT007746.1:CDS
TGTTGGGTGAAGTCAGAATT+AGG 0.300929 2:-22481546 MsaT007746.1:CDS
GCTCGGAACATTGAGTTAAA+TGG 0.362997 2:+22481862 None:intergenic
TTTCTCCTAGATCATTATGT+TGG 0.375290 2:-22481563 MsaT007746.1:intron
CGTATTTGTTGATTGGTCAT+TGG 0.390654 2:-22481212 MsaT007746.1:CDS
TTGATAGCGTATTTGTTGAT+TGG 0.394006 2:-22481219 MsaT007746.1:CDS
TTCATAGTGAATTTGTTGAT+TGG 0.400565 2:-22482015 MsaT007746.1:CDS
GGCCATTGGCACGCATGGAC+TGG 0.408428 2:-22481994 MsaT007746.1:CDS
TTCTCCTAGATCATTATGTT+GGG 0.426785 2:-22481562 MsaT007746.1:intron
TCAGCATCTGGTAAAACAAT+AGG 0.429570 2:-22481178 MsaT007746.1:CDS
GCATGCCAGGACTCAGCATC+TGG 0.452002 2:-22481190 MsaT007746.1:CDS
TCTGTCGATGAAGAATTATC+TGG 0.461758 2:+22481464 None:intergenic
CTGTGGTTGCATTCTATAGC+TGG 0.465799 2:+22482054 None:intergenic
GCACGCATGGACTGGCCATA+TGG 0.467219 2:-22481986 MsaT007746.1:intron
TGATTGGTCATTGGCATGCC+AGG 0.482497 2:-22481203 MsaT007746.1:CDS
TTATAACCTCCTTAAAAGTC+AGG 0.483868 2:+22481281 None:intergenic
AGGAGCGTGGTTGAAATGCT+AGG 0.502214 2:-22481526 MsaT007746.1:CDS
CTATAATCTCTCGATAAGTT+AGG 0.506862 2:+22482077 None:intergenic
GGCCAGTCCATGCGTGCCAA+TGG 0.508439 2:+22481992 None:intergenic
AGAATTATCTCACAAGATAG+AGG 0.512835 2:-22482297 MsaT007746.1:intron
TAGGGTTATCTCAGCAGATA+AGG 0.519446 2:-22481507 MsaT007746.1:CDS
TGATTGGCCATTGGCACGCA+TGG 0.524304 2:-22481999 MsaT007746.1:CDS
CTATGCATGTGTCTCTCCAA+TGG 0.533378 2:-22481775 MsaT007746.1:CDS
TATGCATGTGTCTCTCCAAT+GGG 0.533482 2:-22481774 MsaT007746.1:CDS
ACCTCCTTAAAAGTCAGGCT+CGG 0.541985 2:+22481286 None:intergenic
AGGGTTATCTCAGCAGATAA+GGG 0.550907 2:-22481506 MsaT007746.1:CDS
CAAAAGCAGTGGCTCTACAG+AGG 0.551471 2:+22481108 None:intergenic
TGAATTTGTTGATTGGCCAT+TGG 0.561366 2:-22482008 MsaT007746.1:CDS
AAGCCTTTCTGTTATGTCTG+TGG 0.562011 2:-22481817 MsaT007746.1:CDS
GGAGCGTGGTTGAAATGCTA+GGG 0.571354 2:-22481525 MsaT007746.1:CDS
CTTCCACAGACATAACAGAA+AGG 0.576860 2:+22481814 None:intergenic
TTTGGAACTTGGCGTTGAAG+GGG 0.582633 2:-22481089 MsaT007746.1:CDS
AAAAGCAGTGGCTCTACAGA+GGG 0.599947 2:+22481109 None:intergenic
AGTACCTCGGATGATCCCAT+TGG 0.608676 2:+22481759 None:intergenic
TTGCTTCTGTGCAGTCACAA+AGG 0.625881 2:-22481398 MsaT007746.1:intron
CTCTCCAATGGGATCATCCG+AGG 0.629524 2:-22481763 MsaT007746.1:intron
AAAACTCGTGGGTTGCATTG+CGG 0.634698 2:+22481241 None:intergenic
ACGCCAAGTTCCAAAAGCAG+TGG 0.646767 2:+22481097 None:intergenic
AGAAGCAACACACAATGATG+TGG 0.674691 2:+22481413 None:intergenic
CAATGATGTGGTCAATATGA+CGG 0.678181 2:+22481425 None:intergenic
TGAAGTCAGAATTAGGAGCG+TGG 0.693278 2:-22481539 MsaT007746.1:CDS
AGAGACACATGCATAGTGTG+TGG 0.766106 2:+22481782 None:intergenic
AAAACTGGTGGTGTGCACTG+TGG 0.770580 2:+22482037 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22481078 22482333 22481078 ID=MsaG007746
Chr2 mRNA 22481078 22482333 22481078 ID=MsaT007746.1;Parent=MsaG007746
Chr2 exon 22481078 22481317 22481078 ID=MsaT007746.1.exon5;Parent=MsaT007746.1
Chr2 CDS 22481078 22481317 22481078 ID=cds.MsaT007746.1;Parent=MsaT007746.1
Chr2 exon 22481399 22481575 22481399 ID=MsaT007746.1.exon4;Parent=MsaT007746.1
Chr2 CDS 22481399 22481575 22481399 ID=cds.MsaT007746.1;Parent=MsaT007746.1
Chr2 exon 22481764 22481885 22481764 ID=MsaT007746.1.exon3;Parent=MsaT007746.1
Chr2 CDS 22481764 22481885 22481764 ID=cds.MsaT007746.1;Parent=MsaT007746.1
Chr2 exon 22481987 22482101 22481987 ID=MsaT007746.1.exon2;Parent=MsaT007746.1
Chr2 CDS 22481987 22482101 22481987 ID=cds.MsaT007746.1;Parent=MsaT007746.1
Chr2 exon 22482298 22482333 22482298 ID=MsaT007746.1.exon1;Parent=MsaT007746.1
Chr2 CDS 22482298 22482333 22482298 ID=cds.MsaT007746.1;Parent=MsaT007746.1
Gene Sequence

>MsaT007746.1

ATGGCTGAAAAGCTAGAATTATCTCACAAGATAGAGGGCCTAACTTATCGAGAGATTATAGCCAGCTATAGAATGCAACCACAGTGCACACCACCAGTTTTTCATAGTGAATTTGTTGATTGGCCATTGGCACGCATGGACTGGCCATATGACCATTTAACTCAATGTTCCGAGCCTCCTAAAATATTAACTTTCACAAGCCTTTCTGTTATGTCTGTGGAAGAAGTGTCTGCCACACACTATGCATGTGTCTCTCCAATGGGATCATCCGAGATCATTATGTTGGGTGAAGTCAGAATTAGGAGCGTGGTTGAAATGCTAGGGTTATCTCAGCAGATAAGGGAGAATGTATACAGTCTGTTCCAGATAATTCTTCATCGACAGACATCTCTCTTCTTCAACCGTCATATTGACCACATCATTGTGTGTTGCTTCTGTGCAGTCACAAAGATTTCAAAACCGAGCCTGACTTTTAAGGAGGTTATAAGCATCTATAAGAAGCAACCGCAATGCAACCCACGAGTTTTTGATAGCGTATTTGTTGATTGGTCATTGGCATGCCAGGACTCAGCATCTGGTAAAACAATAGGAAATGAGCATCTCGACATTATTACATTCTGCAATGATATATTTGTTCCCTCTGTAGAGCCACTGCTTTTGGAACTTGGCGTTGAAGGGGAGAAGCTGTGA
Protein sequence

>MsaT007746.1

MAEKLELSHKIEGLTYREIIASYRMQPQCTPPVFHSEFVDWPLARMDWPYDHLTQCSEPPKILTFTSLSVMSVEEVSATHYACVSPMGSSEIIMLGEVRIRSVVEMLGLSQQIRENVYSLFQIILHRQTSLFFNRHIDHIIVCCFCAVTKISKPSLTFKEVISIYKKQPQCNPRVFDSVFVDWSLACQDSASGKTIGNEHLDIITFCNDIFVPSVEPLLLELGVEGEKL*