AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007747


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007747 MtrunA17_Chr8g0353751 55.556 207 90 1 98 302 735 941 4.93e-64 225
MsaG007747 MtrunA17_Chr8g0353751 61.268 142 55 0 389 530 744 885 2.57e-51 189
MsaG007747 MtrunA17_Chr8g0353751 58.947 95 36 2 1 95 555 646 1.35e-25 111
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007747 AT3G12280.2 48.077 208 93 4 98 302 726 921 2.87e-49 183
MsaG007747 AT3G12280.2 56.716 134 54 2 389 522 735 864 1.35e-39 154
MsaG007747 AT3G12280.2 65.169 89 28 2 1 89 547 632 1.94e-25 111
MsaG007747 AT3G12280.1 48.077 208 93 4 98 302 726 921 2.88e-49 183
MsaG007747 AT3G12280.1 56.716 134 54 2 389 522 735 864 1.35e-39 154
MsaG007747 AT3G12280.1 65.169 89 28 2 1 89 547 632 1.94e-25 111

Find 122 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAACTGAACCTGAGTTTC+TGG 0.137982 2:-22488620 MsaT007747.1:CDS
CTTGGGAAAGTTAATATTTC+AGG 0.148410 2:+22487250 None:intergenic
CTCTGTAGAGCCACTGCTTT+TGG 0.167941 2:-22484072 MsaT007747.1:CDS
CCAGAATCTAGTAAAATAAT+AGG 0.219822 2:-22484143 MsaT007747.1:CDS
GGAGAGCATGGCATGGAAAA+AGG 0.224771 2:-22489458 MsaT007747.1:CDS
TTCATTCAAAGAACAACTTT+TGG 0.245558 2:-22489479 MsaT007747.1:CDS
AATCAACAACTTCAGCTAAT+TGG 0.265538 2:+22488385 None:intergenic
AGAGGTGTGTCCGCTGGTTT+AGG 0.315056 2:+22488784 None:intergenic
TTGGGAGCAGTCAGAATAAA+TGG 0.315321 2:-22487913 MsaT007747.1:CDS
ATACGTCCTCCCTTGTCCTT+TGG 0.323788 2:+22488826 None:intergenic
TACGTCCTCCCTTGTCCTTT+GGG 0.358218 2:+22488827 None:intergenic
AGTACCTCAGATGATGAAAA+TGG 0.376291 2:+22488140 None:intergenic
TCTGATGATGTAGGATTCTC+TGG 0.386395 2:+22485324 None:intergenic
CGTATTTGTTGATTGGTCAT+TGG 0.390654 2:-22484177 MsaT007747.1:CDS
TTGATAGCGTATTTGTTGAT+TGG 0.394006 2:-22484184 MsaT007747.1:CDS
AGAAGCAACACAACATGATT+TGG 0.399003 2:+22488742 None:intergenic
AAGATTTCCTGTTATGCATT+GGG 0.406154 2:-22488198 MsaT007747.1:CDS
AAAACTGAACCTGAGTTTCT+GGG 0.413033 2:-22488619 MsaT007747.1:CDS
CAATGCATAACAGGAAATCT+TGG 0.415525 2:+22488200 None:intergenic
TCTGTGGCTTATCATCATTA+TGG 0.417278 2:+22488508 None:intergenic
TGTTTCTCAGATCAGTATGT+TGG 0.421499 2:-22488892 MsaT007747.1:intron
TATGGCCCGCGCCCGCCAAT+GGG 0.433969 2:+22488526 None:intergenic
TGTCTCCTAGATCATTATGT+TGG 0.439372 2:-22485423 MsaT007747.1:intron
TATGCATGTGTCTCTCCAAT+AGG 0.440195 2:-22487187 MsaT007747.1:CDS
CAAGATTTCCTGTTATGCAT+TGG 0.442326 2:-22488199 MsaT007747.1:CDS
AGAAGTCGACTGAAGTATCC+TGG 0.442610 2:+22489505 None:intergenic
GCTCAGGACATTGAGCTAGA+TGG 0.442938 2:+22487275 None:intergenic
CTTTACACTGTTGTGACACC+TGG 0.443475 2:-22487441 MsaT007747.1:CDS
GTTAATATTTCAGGAGGCTC+AGG 0.444282 2:+22487259 None:intergenic
CAGAATCTAGTAAAATAATA+GGG 0.447373 2:-22484142 MsaT007747.1:CDS
GATTGCCCATTGGCGGGCGC+GGG 0.447736 2:-22488531 MsaT007747.1:CDS
GTTTCTCAGATCAGTATGTT+GGG 0.449448 2:-22488891 MsaT007747.1:intron
TGATTGCCCATTGGCGGGCG+CGG 0.456069 2:-22488532 MsaT007747.1:CDS
ATCTGTATGGCCACTGGTGA+TGG 0.459470 2:-22488442 MsaT007747.1:CDS
AGCTGAAGTTGTTGATTCAA+AGG 0.461689 2:-22488379 MsaT007747.1:CDS
CTCTCCAATACATCATCAGC+AGG 0.462992 2:+22489598 None:intergenic
ACACAAGACATTGAGCTAGA+TGG 0.464043 2:+22488243 None:intergenic
AGTACCTCAGATGATCCTAT+TGG 0.469550 2:+22487172 None:intergenic
TGTTTCCTAGATCATTATGT+TGG 0.470021 2:-22487932 MsaT007747.1:intron
GACGGTTGATTTCCTCTGAA+AGG 0.471928 2:+22489395 None:intergenic
GTTTCCTAGATCATTATGTT+GGG 0.473365 2:-22487931 MsaT007747.1:intron
GTCTCCTAGATCATTATGTT+GGG 0.480218 2:-22485422 MsaT007747.1:intron
TAGGGTTATCTCAGGAGATA+AGG 0.480996 2:-22485367 MsaT007747.1:CDS
TTATGGCCCGCGCCCGCCAA+TGG 0.482127 2:+22488525 None:intergenic
TGATTGGTCATTGGCATGCC+AGG 0.482497 2:-22484168 MsaT007747.1:CDS
CGCTCCATCTGTATGGCCAC+TGG 0.485239 2:-22488448 MsaT007747.1:CDS
ACGGTTGATTTCCTCTGAAA+GGG 0.485458 2:+22489396 None:intergenic
ACACATGAAGACTCTTTCCC+AGG 0.486510 2:-22489523 MsaT007747.1:CDS
TTTGTTGATTGCCCATTGGC+GGG 0.491247 2:-22488537 MsaT007747.1:CDS
TCATAGTTTCTGCTGCCTCC+AGG 0.491389 2:+22489323 None:intergenic
AGAATAGTTTCATTGAATGA+TGG 0.491623 2:+22489349 None:intergenic
ACATATTCGCTCCATCTGTA+TGG 0.497154 2:-22488455 MsaT007747.1:CDS
AAGAGCATGGTTGAAATGCT+AGG 0.508698 2:-22485386 MsaT007747.1:CDS
GTTCCCTGCTGATGATGTAT+TGG 0.508997 2:-22489602 MsaT007747.1:CDS
AGAGAGATGTCTGATGATGT+AGG 0.509767 2:+22485315 None:intergenic
GAGATAAGGGAGAATGTATA+CGG 0.509906 2:-22485353 MsaT007747.1:CDS
TCAGAGGAAATCAACCGTCT+TGG 0.513122 2:-22489391 MsaT007747.1:CDS
AATGCATAACAGGAAATCTT+GGG 0.514779 2:+22488201 None:intergenic
GATGATGTATTGGAGAGCTC+TGG 0.517279 2:-22489592 MsaT007747.1:CDS
GAAGTTGTTGATTCAAAGGA+TGG 0.517332 2:-22488375 MsaT007747.1:intron
AGGGTTATCTCAGGAGATAA+GGG 0.517484 2:-22485366 MsaT007747.1:CDS
TGAAGAAGAGGTGTGTCCGC+TGG 0.517765 2:+22488778 None:intergenic
ATTTGTTGATTGCCCATTGG+CGG 0.524796 2:-22488538 MsaT007747.1:CDS
CTATAATCTCCTTAAAACTC+AGG 0.530245 2:+22484246 None:intergenic
TGAAATGCTAGGGTTATCTC+AGG 0.532115 2:-22485375 MsaT007747.1:CDS
TACAATTATCCCAAAGGACA+AGG 0.539877 2:-22488836 MsaT007747.1:CDS
TTGCTTCTATGTAGTCGCAC+AGG 0.540096 2:-22488727 MsaT007747.1:intron
CAGACATAACAGGAAGGCTT+GGG 0.541418 2:+22487233 None:intergenic
ATCACCAGTGGCCATACAGA+TGG 0.542017 2:+22488444 None:intergenic
CAATATGACGATTGAAGAAG+AGG 0.545947 2:+22488766 None:intergenic
ACAGACATAACAGGAAGGCT+TGG 0.546125 2:+22487232 None:intergenic
ATCTCCTTAAAACTCAGGCT+CGG 0.548102 2:+22484251 None:intergenic
CAAAAGCAGTGGCTCTACAG+AGG 0.551471 2:+22484073 None:intergenic
AAGCCTTCCTGTTATGTCTG+TGG 0.553212 2:-22487230 MsaT007747.1:CDS
AAAACGGGTGACCTGCACTC+TGG 0.568545 2:+22488570 None:intergenic
TGTATTTGTTGATTGCCCAT+TGG 0.569139 2:-22488541 MsaT007747.1:CDS
CTATGTAAAGCCGCTGCTGT+CGG 0.569655 2:-22487466 MsaT007747.1:intron
TGCTAGACCGTCCCTTTCAG+AGG 0.571805 2:-22489407 MsaT007747.1:CDS
ACAAACAGCAACCAGAGTGC+AGG 0.572144 2:-22488581 MsaT007747.1:CDS
GTTTCATTGAATGATGGCAT+TGG 0.572221 2:+22489355 None:intergenic
CAATGAAACTATTCTCGACC+TGG 0.573458 2:-22489341 MsaT007747.1:CDS
TTTGGAACTTGGCGCTGAAG+GGG 0.574775 2:-22484054 MsaT007747.1:CDS
TCAGATCAGTATGTTGGGAG+CGG 0.579103 2:-22488886 MsaT007747.1:intron
ATAATTCCAACACGCTTCTG+TGG 0.584901 2:+22488492 None:intergenic
GATAAGCCACAGAAGCGTGT+TGG 0.585393 2:-22488498 MsaT007747.1:CDS
GGGAAAGTTAATATTTCAGG+AGG 0.586410 2:+22487253 None:intergenic
GAATACGCGGCATGGCTGAA+AGG 0.587007 2:-22488860 MsaT007747.1:CDS
AGAGCATGGTTGAAATGCTA+GGG 0.588393 2:-22485385 MsaT007747.1:CDS
GTACAATCTCCCAGAAACTC+AGG 0.592499 2:+22488610 None:intergenic
TGTAATGAAACTTGTGGTGA+TGG 0.594107 2:+22489543 None:intergenic
CTACAACTATCTCAGCAGAC+AGG 0.595751 2:-22487877 MsaT007747.1:CDS
AGCGGTCAGAATACGCGGCA+TGG 0.598188 2:-22488868 MsaT007747.1:CDS
AAAAGCAGTGGCTCTACAGA+GGG 0.599947 2:+22484074 None:intergenic
TTTACACTGTTGTGACACCT+GGG 0.605280 2:-22487440 MsaT007747.1:CDS
TTGATTTCCTCTGAAAGGGA+CGG 0.607373 2:+22489400 None:intergenic
TGCAATCAGAATCAAGAGCA+TGG 0.611351 2:-22485399 MsaT007747.1:CDS
GAAGTCGACTGAAGTATCCT+GGG 0.612687 2:+22489506 None:intergenic
ATAACAGGAAATCTTGGGAA+AGG 0.622579 2:+22488206 None:intergenic
CTTCCACAGACATAACAGGA+AGG 0.636074 2:+22487227 None:intergenic
TTGCTTCTATGCAGTCACAA+AGG 0.637898 2:-22485258 MsaT007747.1:intron
AGCAGTCAGAATAAATGGCA+TGG 0.640293 2:-22487908 MsaT007747.1:CDS
AAAGGCTACAATTATCCCAA+AGG 0.645341 2:-22488842 MsaT007747.1:CDS
GCGCCAAGTTCCAAAAGCAG+TGG 0.653587 2:+22484062 None:intergenic
GTTGAGCAAACAGTCCAAGA+CGG 0.655365 2:+22489377 None:intergenic
GAATTCATTAACAAAGCCGA+TGG 0.655551 2:-22487399 MsaT007747.1:intron
TGACAAAGAGTTATACAATG+AGG 0.657399 2:+22489432 None:intergenic
CTCTCCAATAGGATCATCTG+AGG 0.660685 2:-22487176 MsaT007747.1:intron
ACAATTATCCCAAAGGACAA+GGG 0.662029 2:-22488835 MsaT007747.1:CDS
TTGGGAGCGGTCAGAATACG+CGG 0.673382 2:-22488873 MsaT007747.1:CDS
GTGTAAAGTTCCGACAGCAG+CGG 0.675258 2:+22487456 None:intergenic
TTCAGCGAATCCTAAACCAG+CGG 0.676523 2:-22488794 MsaT007747.1:CDS
CAATGATGTGGTCAATATGA+CGG 0.678181 2:+22485285 None:intergenic
AGAAGCAACACACAATGATG+TGG 0.679428 2:+22485273 None:intergenic
GTGATAAGTTCCATCACCAG+TGG 0.684233 2:+22488432 None:intergenic
TGAAACTATTCTCGACCTGG+AGG 0.688326 2:-22489338 MsaT007747.1:CDS
AAAACACGTGGGTTGCACCG+TGG 0.697661 2:+22484206 None:intergenic
AGAGACACATGCATAGTGTG+CGG 0.699537 2:+22487195 None:intergenic
ACACTGTTGTGACACCTGGG+AGG 0.709129 2:-22487437 MsaT007747.1:CDS
ATTATCCCAAAGGACAAGGG+AGG 0.714855 2:-22488832 MsaT007747.1:CDS
TCTCCAATACATCATCAGCA+GGG 0.719122 2:+22489599 None:intergenic
GCAGCTATAAGAAGCAACCA+CGG 0.739982 2:-22484223 MsaT007747.1:CDS
TTCATGTGTAATGAAACTTG+TGG 0.753409 2:+22489537 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22484034 22489626 22484034 ID=MsaG007747
Chr2 mRNA 22484034 22489626 22484034 ID=MsaT007747.1;Parent=MsaG007747
Chr2 exon 22484034 22484282 22484034 ID=MsaT007747.1.exon9;Parent=MsaT007747.1
Chr2 CDS 22484034 22484282 22484034 ID=cds.MsaT007747.1;Parent=MsaT007747.1
Chr2 exon 22485259 22485435 22485259 ID=MsaT007747.1.exon8;Parent=MsaT007747.1
Chr2 CDS 22485259 22485435 22485259 ID=cds.MsaT007747.1;Parent=MsaT007747.1
Chr2 exon 22487177 22487298 22487177 ID=MsaT007747.1.exon7;Parent=MsaT007747.1
Chr2 CDS 22487177 22487298 22487177 ID=cds.MsaT007747.1;Parent=MsaT007747.1
Chr2 exon 22487400 22487478 22487400 ID=MsaT007747.1.exon6;Parent=MsaT007747.1
Chr2 CDS 22487400 22487478 22487400 ID=cds.MsaT007747.1;Parent=MsaT007747.1
Chr2 exon 22487870 22487944 22487870 ID=MsaT007747.1.exon5;Parent=MsaT007747.1
Chr2 CDS 22487870 22487944 22487870 ID=cds.MsaT007747.1;Parent=MsaT007747.1
Chr2 exon 22488145 22488266 22488145 ID=MsaT007747.1.exon4;Parent=MsaT007747.1
Chr2 CDS 22488145 22488266 22488145 ID=cds.MsaT007747.1;Parent=MsaT007747.1
Chr2 exon 22488376 22488646 22488376 ID=MsaT007747.1.exon3;Parent=MsaT007747.1
Chr2 CDS 22488376 22488646 22488376 ID=cds.MsaT007747.1;Parent=MsaT007747.1
Chr2 exon 22488728 22488904 22488728 ID=MsaT007747.1.exon2;Parent=MsaT007747.1
Chr2 CDS 22488728 22488904 22488728 ID=cds.MsaT007747.1;Parent=MsaT007747.1
Chr2 exon 22489303 22489626 22489303 ID=MsaT007747.1.exon1;Parent=MsaT007747.1
Chr2 CDS 22489303 22489626 22489303 ID=cds.MsaT007747.1;Parent=MsaT007747.1
Gene Sequence

>MsaT007747.1

ATGTTCCCTGCTGATGATGTATTGGAGAGCTCTGGAATTACAGCTTTTGATCTTAGTCATACCATCACCACAAGTTTCATTACACATGAAGACTCTTTCCCAGGATACTTCAGTCGACTTCTCAATTCATTCAAAGAACAACTTTTGGAGAGCATGGCATGGAAAAAGGGTTCCTCATTGTATAACTCTTTGTCACTTGCTAGACCGTCCCTTTCAGAGGAAATCAACCGTCTTGGACTGTTTGCTCAACCAATGCCATCATTCAATGAAACTATTCTCGACCTGGAGGCAGCAGAAACTATGATTGACCTATTTTTCCGTGAGATCAGTATGTTGGGAGCGGTCAGAATACGCGGCATGGCTGAAAGGCTACAATTATCCCAAAGGACAAGGGAGGACGTATACAGTCTTTTTCAGCGAATCCTAAACCAGCGGACACACCTCTTCTTCAATCGTCATATTGACCAAATCATGTTGTGTTGCTTCTATGTAGTCGCACAGTTTTCAAAACTGAACCTGAGTTTCTGGGAGATTGTACACAGCTACAAACAGCAACCAGAGTGCAGGTCACCCGTTTTTCGCAGTGTATTTGTTGATTGCCCATTGGCGGGCGCGGGCCATAATGATGATAAGCCACAGAAGCGTGTTGGAATTATTACATTTTACAATGACATATTCGCTCCATCTGTATGGCCACTGGTGATGGAACTTATCACTGATGAAGAAACTGTGAAAAGTGACCAATTAGCTGAAGTTGTTGATTCAAAGGATGGCCATCTAGCTCAATGTCTTGTGTCTCCAAAAATAGTACCTTTCCCAAGATTTCCTGTTATGCATTGGGAAAAACGATACCCCACAAAAAACTTACGCGTCTCTCCATTTTCATCATCTGAGATCATTATGTTGGGAGCAGTCAGAATAAATGGCATGGTTGAAAAGCTACAACTATCTCAGCAGACAGGAGAGAATCCGCTGCTGTCGGAACTTTACACTGTTGTGACACCTGGGAGGAGTTACAAAATAGCTGAATTCATTAACAAAGCCGATGGCCATCTAGCTCAATGTCCTGAGCCTCCTGAAATATTAACTTTCCCAAGCCTTCCTGTTATGTCTGTGGAAGAAAAGTCTGCCGCACACTATGCATGTGTCTCTCCAATAGGATCATCTGAGATCATTATGTTGGGTGCAATCAGAATCAAGAGCATGGTTGAAATGCTAGGGTTATCTCAGGAGATAAGGGAGAATGTATACGGTCTGTTCCAGAGAATCCTACATCATCAGACATCTCTCTTCTTCAACCGTCATATTGACCACATCATTGTGTGTTGCTTCTATGCAGTCACAAAGATTTCAAAACCGAGCCTGAGTTTTAAGGAGATTATAGGCAGCTATAAGAAGCAACCACGGTGCAACCCACGTGTTTTTGATAGCGTATTTGTTGATTGGTCATTGGCATGCCAGGACCCAGAATCTAGTAAAATAATAGGGCATGAGAATGTCGACATTGTTACATTCTGCAATGATATATTTGTTCCCTCTGTAGAGCCACTGCTTTTGGAACTTGGCGCTGAAGGGGAAGCTGTGAAAAGTTGCTGA

Protein sequence

>MsaT007747.1

MFPADDVLESSGITAFDLSHTITTSFITHEDSFPGYFSRLLNSFKEQLLESMAWKKGSSLYNSLSLARPSLSEEINRLGLFAQPMPSFNETILDLEAAETMIDLFFREISMLGAVRIRGMAERLQLSQRTREDVYSLFQRILNQRTHLFFNRHIDQIMLCCFYVVAQFSKLNLSFWEIVHSYKQQPECRSPVFRSVFVDCPLAGAGHNDDKPQKRVGIITFYNDIFAPSVWPLVMELITDEETVKSDQLAEVVDSKDGHLAQCLVSPKIVPFPRFPVMHWEKRYPTKNLRVSPFSSSEIIMLGAVRINGMVEKLQLSQQTGENPLLSELYTVVTPGRSYKIAEFINKADGHLAQCPEPPEILTFPSLPVMSVEEKSAAHYACVSPIGSSEIIMLGAIRIKSMVEMLGLSQEIRENVYGLFQRILHHQTSLFFNRHIDHIIVCCFYAVTKISKPSLSFKEIIGSYKKQPRCNPRVFDSVFVDWSLACQDPESSKIIGHENVDIVTFCNDIFVPSVEPLLLELGAEGEAVKSC*