AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007760

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007760 MtrunA17_Chr2g0296111 93.473 383 25 0 1 383 1 383 0.0 729
MsaG007760 MtrunA17_Chr2g0296151 66.234 385 118 4 5 382 3 382 0.0 509
MsaG007760 MtrunA17_Chr2g0295741 68.919 370 111 3 15 383 23 389 1.51e-177 499
MsaG007760 MtrunA17_Chr2g0296121 68.108 370 114 3 16 383 102 469 1.42e-172 489
MsaG007760 MtrunA17_Chr2g0295731 64.323 384 111 4 7 374 8 381 1.22e-171 484
MsaG007760 MtrunA17_Chr2g0296221 54.278 374 157 7 17 378 51 422 3.96e-126 370
MsaG007760 MtrunA17_Chr2g0296711 52.957 372 164 4 19 381 28 397 5.80e-126 369
MsaG007760 MtrunA17_Chr4g0067121 54.179 347 153 4 35 379 47 389 1.04e-124 365
MsaG007760 MtrunA17_Chr2g0296741 52.066 363 161 5 22 374 48 407 5.37e-123 362
MsaG007760 MtrunA17_Chr2g0296251 50.390 385 146 6 27 370 18 398 4.89e-122 358
MsaG007760 MtrunA17_Chr2g0296231 51.058 378 176 6 5 378 16 388 1.50e-121 357
MsaG007760 MtrunA17_Chr2g0296721 51.639 366 165 6 22 378 28 390 2.27e-121 357
MsaG007760 MtrunA17_Chr2g0296301 50.282 354 171 3 24 374 19 370 2.91e-121 356
MsaG007760 MtrunA17_Chr2g0315731 52.316 367 162 5 22 378 48 411 2.06e-120 355
MsaG007760 MtrunA17_Chr2g0296171 53.784 370 153 7 17 370 28 395 6.20e-119 351
MsaG007760 MtrunA17_Chr2g0296191 53.994 363 153 7 17 367 634 994 7.20e-115 358
MsaG007760 MtrunA17_Chr2g0296191 51.499 367 167 6 7 369 150 509 4.49e-108 340
MsaG007760 MtrunA17_Chr2g0296291 47.962 417 167 8 1 378 1 406 1.55e-114 340
MsaG007760 MtrunA17_Chr2g0296181 51.902 368 142 8 17 378 27 365 2.35e-112 333
MsaG007760 MtrunA17_Chr2g0295761 49.422 346 169 4 30 373 51 392 7.03e-108 322
MsaG007760 MtrunA17_Chr4g0067141 45.773 343 179 4 29 368 32 370 4.02e-94 286
MsaG007760 MtrunA17_Chr4g0067671 47.003 317 164 2 33 349 38 350 5.68e-88 270
MsaG007760 MtrunA17_Chr2g0286191 47.164 335 166 7 40 370 32 359 2.90e-86 266
MsaG007760 MtrunA17_Chr2g0296161 70.000 170 50 1 214 382 1 170 8.42e-84 252
MsaG007760 MtrunA17_Chr2g0296131 65.497 171 58 1 211 380 2 172 6.21e-75 230
MsaG007760 MtrunA17_Chr2g0296551 58.511 188 75 2 190 376 5 190 1.10e-69 217
MsaG007760 MtrunA17_Chr2g0295711 58.416 202 61 2 1 187 1 194 6.39e-68 213
MsaG007760 MtrunA17_Chr2g0296211 58.989 178 72 1 202 378 189 366 6.73e-66 213
MsaG007760 MtrunA17_Chr2g0296211 45.614 114 56 3 17 125 92 204 1.09e-18 86.7
MsaG007760 MtrunA17_Chr2g0296701 63.125 160 58 1 218 376 3 162 5.22e-65 204
MsaG007760 MtrunA17_Chr2g0295751 68.987 158 46 2 15 172 24 178 7.12e-62 206
MsaG007760 MtrunA17_Chr2g0295721 67.241 116 38 0 214 329 1 116 4.08e-52 169
MsaG007760 MtrunA17_Chr2g0296751 60.741 135 52 1 243 376 1 135 3.76e-50 166
MsaG007760 MtrunA17_Chr4g0001831 32.530 332 209 7 27 350 25 349 3.62e-43 154
MsaG007760 MtrunA17_Chr2g0286221 49.701 167 77 4 216 379 16 178 1.77e-42 146
MsaG007760 MtrunA17_Chr2g0296561 54.688 128 52 3 23 148 20 143 3.71e-34 124
MsaG007760 MtrunA17_Chr8g0377781 29.293 297 203 2 72 364 74 367 1.25e-30 120
MsaG007760 MtrunA17_Chr8g0377801 25.364 343 247 3 34 375 50 384 3.44e-27 111
MsaG007760 MtrunA17_Chr4g0067131 50.617 81 39 1 287 366 1 81 1.62e-18 79.7
MsaG007760 MtrunA17_Chr4g0067681 52.459 61 29 0 276 336 1 61 4.48e-16 72.4
MsaG007760 MtrunA17_Chr8g0339041 22.261 283 198 8 84 355 62 333 4.29e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007760 AT5G07900.1 32.891 377 239 6 2 367 26 399 5.26e-61 202
MsaG007760 AT1G21150.3 30.968 310 207 3 30 335 10 316 2.93e-43 154
MsaG007760 AT1G21150.1 32.000 300 197 3 40 335 56 352 5.36e-43 154
MsaG007760 AT1G21150.2 32.000 300 197 3 40 335 120 416 1.58e-42 154
MsaG007760 AT1G61980.3 29.888 358 200 9 40 355 52 400 3.61e-31 123
MsaG007760 AT1G61980.2 29.888 358 200 9 40 355 52 400 3.61e-31 123
MsaG007760 AT1G61980.1 29.888 358 200 9 40 355 52 400 3.61e-31 123
MsaG007760 AT1G61970.2 28.090 356 199 10 41 351 53 396 4.23e-31 122
MsaG007760 AT1G61970.7 28.090 356 199 10 41 351 53 396 4.23e-31 122
MsaG007760 AT1G61970.6 28.090 356 199 10 41 351 53 396 4.23e-31 122
MsaG007760 AT1G61970.3 28.090 356 199 10 41 351 53 396 4.23e-31 122
MsaG007760 AT1G61970.5 28.090 356 199 10 41 351 53 396 4.23e-31 122
MsaG007760 AT1G61970.4 28.090 356 199 10 41 351 53 396 4.23e-31 122
MsaG007760 AT1G61970.1 28.090 356 199 10 41 351 53 396 4.23e-31 122
MsaG007760 AT1G61990.2 28.701 331 195 5 41 338 53 375 1.14e-30 121
MsaG007760 AT1G61990.1 28.701 331 195 5 41 338 53 375 1.14e-30 121
MsaG007760 AT1G62120.1 29.600 375 204 13 7 331 17 381 1.56e-29 119
MsaG007760 AT5G64950.1 27.508 309 206 5 30 326 26 328 4.08e-29 117
MsaG007760 AT3G46950.1 30.769 286 181 8 7 286 17 291 3.18e-26 109
MsaG007760 AT1G62085.1 26.781 407 204 13 8 330 18 414 5.53e-26 108
MsaG007760 AT1G62085.2 26.781 407 204 13 8 330 18 414 5.53e-26 108
MsaG007760 AT5G23930.1 26.509 381 202 8 40 350 55 427 2.27e-24 104
MsaG007760 AT1G62010.1 27.797 295 169 4 75 331 71 359 3.78e-24 103
MsaG007760 AT1G62110.1 28.421 285 191 6 7 284 17 295 7.87e-23 100
MsaG007760 AT1G61960.1 29.839 248 164 4 41 284 53 294 2.78e-21 95.5
MsaG007760 AT1G62150.1 25.581 258 175 4 34 283 52 300 1.05e-19 90.9
MsaG007760 AT1G79220.1 23.975 317 220 10 76 380 82 389 8.73e-12 66.6

Find 85 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTGGAGGAATTAAAGAATT+TGG 0.111392 2:-22620717 MsaT007760.1:CDS
TATTATTCAGGCTTGCTTTA+AGG 0.167218 2:+22620957 None:intergenic
TTAATTTACTCATGAACTTT+TGG 0.214050 2:-22620520 MsaT007760.1:CDS
TTTCCTGCAACCCTTCTAAA+AGG 0.220666 2:-22621063 MsaT007760.1:CDS
CTCACCTTCTTTAGAAACTA+TGG 0.240194 2:-22621136 MsaT007760.1:CDS
ATTGTAAGTGGAGGAAAGTT+TGG 0.277663 2:+22621195 None:intergenic
CAGGCTTGCTTTAAGGATCT+TGG 0.285333 2:+22620964 None:intergenic
GTGGAGGAATTAAAGAATTT+GGG 0.289340 2:-22620716 MsaT007760.1:CDS
GTTTCTTGATACATGTATAA+AGG 0.301142 2:-22620324 MsaT007760.1:CDS
CCATGTTTAACAATATATTA+TGG 0.307920 2:-22621369 MsaT007760.1:CDS
AGGCTTGCTTTAAGGATCTT+GGG 0.308598 2:+22620965 None:intergenic
ATTCGCAATCCATCTTTCTT+TGG 0.319814 2:-22620866 MsaT007760.1:CDS
AAAGAGGGTTAGGGTTTAGA+AGG 0.320241 2:+22621332 None:intergenic
AGACCAACCCCACTTCTTAA+AGG 0.324708 2:+22620607 None:intergenic
TACATGTATAAAGGGTATTA+AGG 0.329386 2:-22620315 MsaT007760.1:CDS
GACACATGACATGCTGAAAT+TGG 0.353711 2:-22620738 MsaT007760.1:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2:-22620501 MsaT007760.1:CDS
TAATGTTGGAAAAGAGGGTT+AGG 0.372467 2:+22621322 None:intergenic
ACTCCTTCAAAATCAACCTT+TGG 0.392434 2:-22620689 MsaT007760.1:CDS
TTTCTTGATACATGTATAAA+GGG 0.393177 2:-22620323 MsaT007760.1:CDS
TAGCCATGGTTCCTTTGAAA+TGG 0.395647 2:+22621087 None:intergenic
GTCAGAGTTAGAAGCACCTT+TGG 0.395726 2:+22621012 None:intergenic
ATGTTCTACAAGCATTTAGA+AGG 0.400394 2:-22620577 MsaT007760.1:CDS
CTGAAGTGTTGGTGCAGAAA+TGG 0.408928 2:+22621281 None:intergenic
TTGCAATATCTTCTGAAGAA+AGG 0.412458 2:-22620401 MsaT007760.1:CDS
TGAAATGGTAAGACGTAGTT+GGG 0.418042 2:+22621102 None:intergenic
AGCTTCTCAGGCACTACAAA+TGG 0.425476 2:+22620347 None:intergenic
GAATCTATAAATCAATTGAT+AGG 0.434987 2:+22620922 None:intergenic
TTATCTATTAAAGTTGTATG+AGG 0.446208 2:-22620285 MsaT007760.1:CDS
TTTCTTTAAGTTTCGAAAAG+AGG 0.455763 2:-22620445 MsaT007760.1:CDS
AAAAGGGTAATGAGTAATGT+TGG 0.457470 2:+22621308 None:intergenic
TTGAAATGGTAAGACGTAGT+TGG 0.462482 2:+22621101 None:intergenic
AATGCTTTGCGTAAGGAGTC+AGG 0.470114 2:-22620774 MsaT007760.1:CDS
GTATCAAGAAACAGCTTCTC+AGG 0.470819 2:+22620335 None:intergenic
GAAAGTTTGGAAGCAGATTG+TGG 0.472463 2:+22621208 None:intergenic
CTTGAAACAAACATTGTAAG+TGG 0.473763 2:+22621183 None:intergenic
AAGAAGGTGAGAACAGAGTC+AGG 0.478602 2:+22621148 None:intergenic
TCCTACCTCATCAACAACTT+TGG 0.478665 2:-22621238 MsaT007760.1:CDS
TTTGGGTCAATCAGTTGGGT+TGG 0.480856 2:-22620502 MsaT007760.1:CDS
TTCCTGCAACCCTTCTAAAA+GGG 0.482738 2:-22621062 MsaT007760.1:CDS
GTGCAGAAATGGAGAGAAAA+GGG 0.484129 2:+22621292 None:intergenic
GTTTGAGTCGGCCACTCCAT+TGG 0.486888 2:+22620805 None:intergenic
AAAGTTTGGAAGCAGATTGT+GGG 0.488544 2:+22621209 None:intergenic
GAAACCATAGTTTCTAAAGA+AGG 0.490559 2:+22621132 None:intergenic
GGCTTGCTTTAAGGATCTTG+GGG 0.492460 2:+22620966 None:intergenic
TTCTTTAAGTTTCGAAAAGA+GGG 0.495393 2:-22620444 MsaT007760.1:CDS
AATGAGTAATGTTGGAAAAG+AGG 0.500309 2:+22621316 None:intergenic
CCATAATATATTGTTAAACA+TGG 0.502176 2:+22621369 None:intergenic
GGTGCAGAAATGGAGAGAAA+AGG 0.506359 2:+22621291 None:intergenic
ATGAGTAATGTTGGAAAAGA+GGG 0.517078 2:+22621317 None:intergenic
TTAAAAGAATGCTTTGCGTA+AGG 0.521144 2:-22620781 MsaT007760.1:CDS
TCGAAAAGAGGGTCCTTCCA+AGG 0.522254 2:-22620433 MsaT007760.1:CDS
ATTATATTGCTGATTGCCAA+TGG 0.523498 2:-22620821 MsaT007760.1:CDS
CGAGCGTCTCCAAAGAAAGA+TGG 0.526080 2:+22620857 None:intergenic
AAACTGCTGGTCCTTTGGCA+AGG 0.527369 2:+22620468 None:intergenic
AAGAGGGTTAGGGTTTAGAA+GGG 0.536351 2:+22621333 None:intergenic
ACTACGTCTTACCATTTCAA+AGG 0.541064 2:-22621098 MsaT007760.1:CDS
GGCTAGCAACGCTACACCAA+AGG 0.553591 2:+22620673 None:intergenic
AGTCGATGCCTTTAAGAAGT+GGG 0.559365 2:-22620615 MsaT007760.1:CDS
AACTGCTGGTCCTTTGGCAA+GGG 0.562420 2:+22620469 None:intergenic
GAGGTAGGAGACTGCAAAAG+AGG 0.562960 2:+22621252 None:intergenic
GATGCACTTGCCCTTGCCAA+AGG 0.563336 2:-22620479 MsaT007760.1:CDS
TATTGCAATACAGAGGCCCT+TGG 0.565124 2:+22620416 None:intergenic
AGAGGTCGAATCTGAAGTGT+TGG 0.569312 2:+22621270 None:intergenic
AATGTTGGAAAAGAGGGTTA+GGG 0.572620 2:+22621323 None:intergenic
CGAAAAGAGGGTCCTTCCAA+GGG 0.576545 2:-22620432 MsaT007760.1:CDS
AAGTCGATGCCTTTAAGAAG+TGG 0.577016 2:-22620616 MsaT007760.1:CDS
TAGATTCTTGCAATCAGACA+AGG 0.583953 2:-22620906 MsaT007760.1:CDS
GCAATACAGAGGCCCTTGGA+AGG 0.596897 2:+22620420 None:intergenic
ATGCCTTTAAGAAGTGGGGT+TGG 0.598873 2:-22620610 MsaT007760.1:CDS
GCCAAAGTTGTTGATGAGGT+AGG 0.602091 2:+22621237 None:intergenic
GTCGATGCCTTTAAGAAGTG+GGG 0.602513 2:-22620614 MsaT007760.1:CDS
ACATGACATGCTGAAATTGG+TGG 0.610610 2:-22620735 MsaT007760.1:CDS
AAGTTTGGAAGCAGATTGTG+GGG 0.614108 2:+22621210 None:intergenic
TGACATGCTGAAATTGGTGG+AGG 0.631062 2:-22620732 MsaT007760.1:CDS
AAAAGCCAAAGTTGTTGATG+AGG 0.632517 2:+22621233 None:intergenic
TTTCTTTGGAGACGCTCGTG+TGG 0.632941 2:-22620852 MsaT007760.1:CDS
ACTCAATCTTGCTTACACCA+TGG 0.635583 2:-22620258 MsaT007760.1:CDS
AATTTCAGCATGTCATGTGT+CGG 0.639746 2:+22620740 None:intergenic
GGGTTGCAGGAAAGTAGCCA+TGG 0.641042 2:+22621073 None:intergenic
GAAACAAACATTGTAAGTGG+AGG 0.650981 2:+22621186 None:intergenic
TTACCATTTCAAAGGAACCA+TGG 0.651356 2:-22621090 MsaT007760.1:CDS
ATGGATGTTAACATACAATG+AGG 0.655070 2:+22620551 None:intergenic
ATTGCTGATTGCCAATGGAG+TGG 0.669284 2:-22620816 MsaT007760.1:CDS
CAGAAGATATTGCAATACAG+AGG 0.669772 2:+22620409 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22620238 22621389 22620238 ID=MsaG007760
Chr2 mRNA 22620238 22621389 22620238 ID=MsaT007760.1;Parent=MsaG007760
Chr2 exon 22620238 22621389 22620238 ID=MsaT007760.1.exon1;Parent=MsaT007760.1
Chr2 CDS 22620238 22621389 22620238 ID=cds.MsaT007760.1;Parent=MsaT007760.1
Gene Sequence

>MsaT007760.1

ATGTTTAACAATATATTATGGCGACACAGAACAACCCTTCTAAACCCTAACCCTCTTTTCCAACATTACTCATTACCCTTTTCTCTCCATTTCTGCACCAACACTTCAGATTCGACCTCTTTTGCAGTCTCCTACCTCATCAACAACTTTGGCTTTTCCCCACAATCTGCTTCCAAACTTTCCTCCACTTACAATGTTTGTTTCAAGAATGCCCAAAAACCTGACTCTGTTCTCACCTTCTTTAGAAACTATGGTTTCTCAGATTCCCAACTACGTCTTACCATTTCAAAGGAACCATGGCTACTTTCCTGCAACCCTTCTAAAAGGGTTTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTAACTCTGACATTGTTAACATTGTCACTAAGAACCCCAAGATCCTTAAAGCAAGCCTGAATAATAAAATTATCAAAGCCTATCAATTGATTTATAGATTCTTGCAATCAGACAAGGTCATTATTGATTGTGTTATTCGCAATCCATCTTTCTTTGGAGACGCTCGTGTGGTATCTAACATTATATTGCTGATTGCCAATGGAGTGGCCGACTCAAACATTAAAAGAATGCTTTGCGTAAGGAGTCAGGCATTTCAGACACCGACACATGACATGCTGAAATTGGTGGAGGAATTAAAGAATTTGGGTTTTACTCCTTCAAAATCAACCTTTGGTGTAGCGTTGCTAGCCAAACTATCTGTAAGAAAAACATTGTGGGGAGAGAAAGTCGATGCCTTTAAGAAGTGGGGTTGGTCTGATGAAGATGTTCTACAAGCATTTAGAAGGCAACCTCATTGTATGTTAACATCCATTGATAAAATTAATTTACTCATGAACTTTTGGGTCAATCAGTTGGGTTGGGATGCACTTGCCCTTGCCAAAGGACCAGCAGTTTTTTCTTTAAGTTTCGAAAAGAGGGTCCTTCCAAGGGCCTCTGTATTGCAATATCTTCTGAAGAAAGGTTTGCGAAACAAGAATGCAAGCTTAACTTGTCCATTTGTAGTGCCTGAGAAGCTGTTTCTTGATACATGTATAAAGGGTATTAAGGAGTCTTCTTATCTATTAAAGTTGTATGAGGAAAAACTCAATCTTGCTTACACCATGGACAAAACATGCACATCATGA
Protein sequence

>MsaT007760.1

MFNNILWRHRTTLLNPNPLFQHYSLPFSLHFCTNTSDSTSFAVSYLINNFGFSPQSASKLSSTYNVCFKNAQKPDSVLTFFRNYGFSDSQLRLTISKEPWLLSCNPSKRVLPKFQFFLSKGASNSDIVNIVTKNPKILKASLNNKIIKAYQLIYRFLQSDKVIIDCVIRNPSFFGDARVVSNIILLIANGVADSNIKRMLCVRSQAFQTPTHDMLKLVEELKNLGFTPSKSTFGVALLAKLSVRKTLWGEKVDAFKKWGWSDEDVLQAFRRQPHCMLTSIDKINLLMNFWVNQLGWDALALAKGPAVFSLSFEKRVLPRASVLQYLLKKGLRNKNASLTCPFVVPEKLFLDTCIKGIKESSYLLKLYEEKLNLAYTMDKTCTS*