AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007761

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007761 MtrunA17_Chr2g0295741 65.206 388 129 2 1 382 1 388 2.89e-180 506
MsaG007761 MtrunA17_Chr2g0296151 63.613 382 139 0 1 382 1 382 2.96e-176 495
MsaG007761 MtrunA17_Chr2g0296111 64.247 372 127 3 14 380 10 380 8.23e-168 474
MsaG007761 MtrunA17_Chr2g0296121 62.404 391 137 2 1 382 79 468 3.27e-167 476
MsaG007761 MtrunA17_Chr2g0295731 61.828 372 140 1 5 374 10 381 5.05e-163 462
MsaG007761 MtrunA17_Chr4g0067121 50.145 345 169 2 36 379 47 389 6.18e-123 360
MsaG007761 MtrunA17_Chr2g0296131 94.857 175 9 0 209 383 1 175 1.59e-121 348
MsaG007761 MtrunA17_Chr2g0296721 48.711 388 192 3 2 382 7 394 8.65e-121 355
MsaG007761 MtrunA17_Chr2g0296301 48.883 358 177 3 24 377 18 373 7.79e-120 352
MsaG007761 MtrunA17_Chr2g0296251 50.000 376 148 4 33 370 25 398 4.74e-119 350
MsaG007761 MtrunA17_Chr2g0296291 48.837 387 158 3 35 383 27 411 7.62e-118 348
MsaG007761 MtrunA17_Chr2g0296741 47.815 389 195 3 2 382 27 415 8.75e-118 348
MsaG007761 MtrunA17_Chr2g0296711 50.872 344 166 1 42 382 55 398 1.30e-115 343
MsaG007761 MtrunA17_Chr2g0296221 50.278 360 170 3 32 382 67 426 4.13e-115 342
MsaG007761 MtrunA17_Chr2g0295761 47.462 394 183 7 1 373 2 392 2.04e-113 336
MsaG007761 MtrunA17_Chr2g0315731 47.301 389 197 3 2 382 27 415 2.23e-113 337
MsaG007761 MtrunA17_Chr2g0296231 47.922 361 180 3 25 382 37 392 8.63e-111 330
MsaG007761 MtrunA17_Chr2g0296191 50.000 348 170 2 22 369 166 509 2.03e-108 341
MsaG007761 MtrunA17_Chr2g0296191 48.459 357 174 4 21 367 638 994 1.82e-100 320
MsaG007761 MtrunA17_Chr2g0296171 50.898 334 159 2 42 370 62 395 3.72e-107 321
MsaG007761 MtrunA17_Chr4g0067141 46.471 340 178 2 31 368 33 370 3.41e-104 312
MsaG007761 MtrunA17_Chr4g0067671 46.389 360 185 5 1 355 1 357 1.66e-102 307
MsaG007761 MtrunA17_Chr2g0296181 46.610 354 159 3 32 382 43 369 8.57e-99 299
MsaG007761 MtrunA17_Chr2g0286191 46.269 335 168 7 41 370 32 359 2.20e-96 291
MsaG007761 MtrunA17_Chr2g0296161 61.446 166 64 0 215 380 3 168 5.35e-72 222
MsaG007761 MtrunA17_Chr2g0295711 61.616 198 64 3 1 189 1 195 2.31e-71 222
MsaG007761 MtrunA17_Chr2g0296551 53.125 192 90 0 191 382 5 196 6.79e-66 207
MsaG007761 MtrunA17_Chr2g0295751 65.341 176 54 3 1 169 1 176 1.27e-64 214
MsaG007761 MtrunA17_Chr2g0296701 57.229 166 71 0 217 382 3 168 3.07e-64 202
MsaG007761 MtrunA17_Chr2g0296211 49.754 203 99 1 183 382 168 370 1.02e-62 205
MsaG007761 MtrunA17_Chr2g0296751 58.088 136 57 0 242 377 1 136 1.04e-52 172
MsaG007761 MtrunA17_Chr2g0295721 70.642 109 32 0 217 325 5 113 6.83e-52 169
MsaG007761 MtrunA17_Chr4g0001831 31.988 347 227 5 28 368 25 368 5.32e-50 172
MsaG007761 MtrunA17_Chr8g0377781 31.757 296 199 2 72 364 73 368 4.20e-41 149
MsaG007761 MtrunA17_Chr2g0286221 44.937 158 85 2 217 374 18 173 7.24e-41 142
MsaG007761 MtrunA17_Chr2g0296561 52.941 136 55 3 25 160 23 149 1.34e-33 122
MsaG007761 MtrunA17_Chr8g0377801 26.349 315 228 3 71 381 85 399 1.31e-31 123
MsaG007761 MtrunA17_Chr4g0067131 54.321 81 37 0 286 366 1 81 1.32e-25 99.0
MsaG007761 MtrunA17_Chr4g0067681 55.738 61 27 0 275 335 1 61 1.61e-17 76.3
MsaG007761 MtrunA17_Chr8g0339041 22.414 290 214 7 86 368 63 348 6.06e-13 69.7
MsaG007761 MtrunA17_Chr3g0127891 23.308 266 182 6 95 348 236 491 3.72e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007761 AT5G07900.1 33.934 333 212 4 41 367 69 399 1.98e-61 203
MsaG007761 AT1G21150.1 31.627 332 217 6 41 365 56 384 2.87e-46 163
MsaG007761 AT1G21150.3 30.321 343 229 6 30 365 9 348 3.82e-46 162
MsaG007761 AT1G21150.2 31.627 332 217 6 41 365 120 448 1.53e-45 162
MsaG007761 AT1G61980.3 30.081 369 211 8 41 367 52 415 4.14e-43 155
MsaG007761 AT1G61980.2 30.081 369 211 8 41 367 52 415 4.14e-43 155
MsaG007761 AT1G61980.1 30.081 369 211 8 41 367 52 415 4.14e-43 155
MsaG007761 AT1G62085.1 29.024 410 206 10 27 353 32 439 1.68e-39 146
MsaG007761 AT1G62085.2 29.024 410 206 10 27 353 32 439 1.68e-39 146
MsaG007761 AT3G46950.1 28.224 411 204 10 22 353 32 430 7.36e-39 144
MsaG007761 AT1G62120.1 29.219 397 227 9 5 355 19 407 3.41e-38 142
MsaG007761 AT1G61990.2 27.981 411 239 7 5 367 7 408 1.29e-37 140
MsaG007761 AT1G61990.1 27.981 411 239 7 5 367 7 408 1.29e-37 140
MsaG007761 AT1G61970.2 28.571 406 220 10 5 353 7 399 5.87e-37 139
MsaG007761 AT1G61970.7 28.571 406 220 10 5 353 7 399 5.87e-37 139
MsaG007761 AT1G61970.6 28.571 406 220 10 5 353 7 399 5.87e-37 139
MsaG007761 AT1G61970.3 28.571 406 220 10 5 353 7 399 5.87e-37 139
MsaG007761 AT1G61970.5 28.571 406 220 10 5 353 7 399 5.87e-37 139
MsaG007761 AT1G61970.4 28.571 406 220 10 5 353 7 399 5.87e-37 139
MsaG007761 AT1G61970.1 28.571 406 220 10 5 353 7 399 5.87e-37 139
MsaG007761 AT5G64950.1 30.966 352 211 10 30 359 25 366 6.01e-35 132
MsaG007761 AT1G61960.1 26.044 407 212 8 42 367 53 451 6.54e-33 128
MsaG007761 AT1G62110.1 26.923 390 206 7 41 353 52 439 2.52e-32 127
MsaG007761 AT1G62150.1 25.750 400 219 9 35 359 52 448 5.38e-31 123
MsaG007761 AT5G23930.1 27.506 389 204 7 41 358 55 436 1.24e-29 119
MsaG007761 AT1G62010.1 26.331 357 188 7 54 359 57 389 1.25e-28 115
MsaG007761 AT1G56380.2 25.135 370 213 11 42 359 28 385 2.11e-23 100
MsaG007761 AT1G56380.1 25.410 366 209 11 42 355 17 370 2.46e-23 100
MsaG007761 AT1G79220.1 23.701 308 192 9 79 380 119 389 4.31e-12 67.4

Find 76 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATACCTCATTCACAATTT+TGG 0.106320 2:+22631905 MsaT007761.1:CDS
GTGAAGGAGTTAAAGGATTT+AGG 0.142526 2:+22632421 MsaT007761.1:CDS
AATTGCAATCCAACTTTATT+TGG 0.152258 2:+22632277 MsaT007761.1:CDS
TTGCAATGACAGCGCGTATT+TGG 0.169327 2:-22632042 None:intergenic
TGCTAAAACACCAAGGGTTT+TGG 0.198044 2:+22632672 MsaT007761.1:CDS
TAAACTCAGTGATGAGCTTT+TGG 0.230631 2:+22632617 MsaT007761.1:CDS
TTTCTTCATAAAGCTTTAAT+AGG 0.257224 2:-22632858 None:intergenic
GAAGAGAGGTTTGCATAAAA+AGG 0.278062 2:+22632750 MsaT007761.1:CDS
TGAAGGAGTTAAAGGATTTA+GGG 0.287415 2:+22632422 MsaT007761.1:CDS
AGACCAGCCCCATTTCTTAA+AGG 0.311413 2:-22632530 None:intergenic
AAACTTTGGCAACACCCTTT+TGG 0.315392 2:-22632095 None:intergenic
AAATATTGCAAGATTGCTTC+GGG 0.322556 2:+22632357 MsaT007761.1:CDS
GGTTTCGGATAAATATGTTT+TGG 0.343580 2:-22632485 None:intergenic
TGAGTTGGTGAAGGAGTTAA+AGG 0.346015 2:+22632414 MsaT007761.1:CDS
AGTTGATACCTTTAAGAAAT+GGG 0.353795 2:+22632522 MsaT007761.1:CDS
AAGTTGATACCTTTAAGAAA+TGG 0.365182 2:+22632521 MsaT007761.1:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2:+22632636 MsaT007761.1:CDS
TGCAATGACAGCGCGTATTT+GGG 0.367219 2:-22632041 None:intergenic
AATAGTCCCAACCTATGAAT+TGG 0.380595 2:+22632210 MsaT007761.1:CDS
CTACTTCTTCCAAATAAAGT+TGG 0.386610 2:-22632286 None:intergenic
CATGTATATAAATCGGTTTA+AGG 0.388072 2:+22632822 MsaT007761.1:CDS
GTCAGAGTTAGAAGCACCTT+TGG 0.395726 2:-22632131 None:intergenic
TAAATATTGCAAGATTGCTT+CGG 0.406570 2:+22632356 MsaT007761.1:CDS
GTATTTATAAACCAATTCAT+AGG 0.407861 2:-22632221 None:intergenic
AAGAAGTTGAGAACTGAATC+AGG 0.411972 2:-22631995 None:intergenic
TATAAACCAATTCATAGGTT+GGG 0.416412 2:-22632216 None:intergenic
TATGTTCCTTACTGCAAGTC+TGG 0.423384 2:+22632180 MsaT007761.1:CDS
GTCTCATCAGTGACTATAAA+CGG 0.428293 2:-22632790 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.429067 2:+22632635 MsaT007761.1:CDS
TCTATGACATGTATATAAAT+CGG 0.442482 2:+22632815 MsaT007761.1:CDS
GTGGAGAAATAGTGAGAAAA+GGG 0.466735 2:-22631851 None:intergenic
GGTGGAGAAATAGTGAGAAA+AGG 0.473547 2:-22631852 None:intergenic
TGGGTTTGGTGAGAAAGTTA+AGG 0.482786 2:-22631816 None:intergenic
TTTAACTCCTTCACCAACTC+AGG 0.483502 2:-22632412 None:intergenic
AAATAGTGAGAAAAGGGTAA+TGG 0.487797 2:-22631845 None:intergenic
TTATAAACCAATTCATAGGT+TGG 0.490431 2:-22632217 None:intergenic
TCTTGTCCAGACTTGCAGTA+AGG 0.497150 2:-22632186 None:intergenic
TAAGGTCTTGAGATAGAGAA+GGG 0.500312 2:-22631798 None:intergenic
TTTCCTACGATCCCTCCAAA+AGG 0.504365 2:+22632080 MsaT007761.1:CDS
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2:+22632645 MsaT007761.1:CDS
TATGATCCTTCGCTCCAGAC+TGG 0.512261 2:-22632698 None:intergenic
CTCAACTTCTTTAGAAACCA+TGG 0.514218 2:+22632007 MsaT007761.1:CDS
AGAATCCAAAATTGTGAATG+AGG 0.517196 2:-22631910 None:intergenic
TTAAGGTCTTGAGATAGAGA+AGG 0.519722 2:-22631799 None:intergenic
CTTGAAATGAACATTGTAAG+TGG 0.523656 2:-22631960 None:intergenic
AGACTTGCAGTAAGGAACAT+AGG 0.526410 2:-22632178 None:intergenic
TATTCCACAACTGAGGCTCT+TGG 0.536781 2:-22632721 None:intergenic
GACTTGCAGTAAGGAACATA+GGG 0.539419 2:-22632177 None:intergenic
TTCCTACGATCCCTCCAAAA+GGG 0.559061 2:+22632081 MsaT007761.1:CDS
ATACCTTTAAGAAATGGGGC+TGG 0.564816 2:+22632527 MsaT007761.1:CDS
GTTGATACCTTTAAGAAATG+GGG 0.574983 2:+22632523 MsaT007761.1:CDS
GTGGAATATCTTCTGAAGAG+AGG 0.579571 2:+22632736 MsaT007761.1:CDS
CATATTTATCCGAAACCCGA+TGG 0.584129 2:+22632491 MsaT007761.1:CDS
ATCAAATTGTTGATTCAGAG+TGG 0.585441 2:+22632322 MsaT007761.1:CDS
GGTTGAATCTGAAGTGTTGG+TGG 0.589706 2:-22631870 None:intergenic
CAACTTGCCTGAGTTGGTGA+AGG 0.601746 2:+22632405 MsaT007761.1:CDS
TTTGGGAATCAGAGAAACCA+TGG 0.602265 2:-22632024 None:intergenic
ATTGAATCTTGCACAAACCA+AGG 0.602352 2:+22632882 MsaT007761.1:CDS
GGCCATTGCTAAAACACCAA+GGG 0.609965 2:+22632666 MsaT007761.1:CDS
GAGGTATGACACTGCAAAGG+AGG 0.613172 2:-22631891 None:intergenic
GGAGGTTGAATCTGAAGTGT+TGG 0.620260 2:-22631873 None:intergenic
TGAGATAGAGAAGGGTTGAA+CGG 0.624735 2:-22631790 None:intergenic
GACAGGCAACTTGCCTGAGT+TGG 0.629550 2:+22632399 MsaT007761.1:CDS
CATACCAAGAGCCTCAGTTG+TGG 0.635286 2:+22632717 MsaT007761.1:CDS
ACATAGGGTTCTTAGTGACA+AGG 0.636619 2:-22632162 None:intergenic
ACTGAGAAAATTGATTGTGA+AGG 0.638960 2:-22632451 None:intergenic
AATACGCGCTGTCATTGCAA+AGG 0.640391 2:+22632045 MsaT007761.1:CDS
TTTATTTGGAAGAAGTAGCG+TGG 0.642616 2:+22632291 MsaT007761.1:CDS
GAAATGAACATTGTAAGTGG+AGG 0.651609 2:-22631957 None:intergenic
GGATCGTAGGAAAGCAAGCG+TGG 0.652763 2:-22632070 None:intergenic
GTATATAAATCGGTTTAAGG+AGG 0.668406 2:+22632825 MsaT007761.1:CDS
CAGAAGATATTCCACAACTG+AGG 0.677963 2:-22632728 None:intergenic
TGGCCATTGCTAAAACACCA+AGG 0.690744 2:+22632665 MsaT007761.1:CDS
AATATTGCAAGATTGCTTCG+GGG 0.721927 2:+22632358 MsaT007761.1:CDS
AATGAGGTATGACACTGCAA+AGG 0.727461 2:-22631894 None:intergenic
ATGGATGTTAACATACACTG+AGG 0.775832 2:-22632586 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22631773 22632924 22631773 ID=MsaG007761
Chr2 mRNA 22631773 22632924 22631773 ID=MsaT007761.1;Parent=MsaG007761
Chr2 exon 22631773 22632924 22631773 ID=MsaT007761.1.exon1;Parent=MsaT007761.1
Chr2 CDS 22631773 22632924 22631773 ID=cds.MsaT007761.1;Parent=MsaT007761.1
Gene Sequence

>MsaT007761.1

ATGTTTAACACTCATCTCCGTTCAACCCTTCTCTATCTCAAGACCTTAACTTTCTCACCAAACCCAAAAACTCCATTACCCTTTTCTCACTATTTCTCCACCAACACTTCAGATTCAACCTCCTTTGCAGTGTCATACCTCATTCACAATTTTGGATTCTCCCCACAATTTGCTTCAAAACTTTCCTCCACTTACAATGTTCATTTCAAGACTCCCCGAAAACCTGATTCAGTTCTCAACTTCTTTAGAAACCATGGTTTCTCTGATTCCCAAATACGCGCTGTCATTGCAAAGGAACCACGCTTGCTTTCCTACGATCCCTCCAAAAGGGTGTTGCCAAAGTTTCAATTTTTACTCTCCAAAGGTGCTTCTAACTCTGACATTGTTAACCTTGTCACTAAGAACCCTATGTTCCTTACTGCAAGTCTGGACAAGAAAATAGTCCCAACCTATGAATTGGTTTATAAATACTTGCAATCTGATAAACACATTCTTGCTTTGTTGAATTGCAATCCAACTTTATTTGGAAGAAGTAGCGTGGAACATAACATCAAATTGTTGATTCAGAGTGGAGTGACAGACATAAATATTGCAAGATTGCTTCGGGGCAATATTAAGTTTTTCATGACAGGCAACTTGCCTGAGTTGGTGAAGGAGTTAAAGGATTTAGGGTTTCATCCTTCACAATCAATTTTCTCAGTAGCATTGATTGCCAAAACATATTTATCCGAAACCCGATGGAATGAAAAAGTTGATACCTTTAAGAAATGGGGCTGGTCTGATGAAGATGTTCTTGAAGCATTTAAAAAGCAACCTCAGTGTATGTTAACATCCATTGATAAAATAAACTCAGTGATGAGCTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCTAAAACACCAAGGGTTTTGGGGGCCAGTCTGGAGCGAAGGATCATACCAAGAGCCTCAGTTGTGGAATATCTTCTGAAGAGAGGTTTGCATAAAAAGGAAGCAAGTTTAACTTCTCCGTTTATAGTCACTGATGAGACATTCTATGACATGTATATAAATCGGTTTAAGGAGGAGTCTTCTTACCTATTAAAGCTTTATGAAGAAAAATTGAATCTTGCACAAACCAAGGAAAAAACTGGCATGATATGA
Protein sequence

>MsaT007761.1

MFNTHLRSTLLYLKTLTFSPNPKTPLPFSHYFSTNTSDSTSFAVSYLIHNFGFSPQFASKLSSTYNVHFKTPRKPDSVLNFFRNHGFSDSQIRAVIAKEPRLLSYDPSKRVLPKFQFLLSKGASNSDIVNLVTKNPMFLTASLDKKIVPTYELVYKYLQSDKHILALLNCNPTLFGRSSVEHNIKLLIQSGVTDINIARLLRGNIKFFMTGNLPELVKELKDLGFHPSQSIFSVALIAKTYLSETRWNEKVDTFKKWGWSDEDVLEAFKKQPQCMLTSIDKINSVMSFWVNQLGWDAMAIAKTPRVLGASLERRIIPRASVVEYLLKRGLHKKEASLTSPFIVTDETFYDMYINRFKEESSYLLKLYEEKLNLAQTKEKTGMI*