AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007762


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007762 MtrunA17_Chr2g0295731 75.578 389 85 3 1 387 1 381 0.0 580
MsaG007762 MtrunA17_Chr2g0296151 75.521 384 84 2 9 392 6 379 0.0 580
MsaG007762 MtrunA17_Chr2g0296111 66.582 395 115 4 1 393 1 380 0.0 518
MsaG007762 MtrunA17_Chr2g0295741 65.394 393 127 4 1 392 1 385 1.90e-179 504
MsaG007762 MtrunA17_Chr2g0296121 66.062 386 127 2 9 393 84 466 1.50e-176 500
MsaG007762 MtrunA17_Chr4g0067121 52.632 342 159 2 52 392 50 389 7.67e-125 365
MsaG007762 MtrunA17_Chr2g0296221 50.909 385 178 6 18 391 38 422 2.71e-124 366
MsaG007762 MtrunA17_Chr2g0296251 49.608 383 151 4 41 383 18 398 2.26e-121 357
MsaG007762 MtrunA17_Chr2g0296711 51.754 342 162 2 55 393 55 396 8.56e-121 357
MsaG007762 MtrunA17_Chr2g0296721 50.000 364 173 3 36 391 28 390 7.85e-119 350
MsaG007762 MtrunA17_Chr2g0296301 48.718 351 174 4 41 387 22 370 5.21e-118 348
MsaG007762 MtrunA17_Chr2g0296741 52.522 337 155 3 55 387 72 407 2.44e-117 347
MsaG007762 MtrunA17_Chr2g0315731 49.727 366 164 4 36 387 48 407 1.84e-116 345
MsaG007762 MtrunA17_Chr2g0296231 48.168 382 190 5 14 391 11 388 1.08e-115 342
MsaG007762 MtrunA17_Chr2g0296171 50.404 371 163 5 31 383 28 395 5.20e-115 341
MsaG007762 MtrunA17_Chr2g0296291 45.906 403 172 4 27 391 12 406 3.62e-113 337
MsaG007762 MtrunA17_Chr2g0296191 50.000 374 176 6 18 380 621 994 1.20e-109 345
MsaG007762 MtrunA17_Chr2g0296191 49.153 354 176 2 29 382 160 509 4.21e-105 333
MsaG007762 MtrunA17_Chr2g0296181 47.956 367 157 5 31 391 27 365 1.21e-104 314
MsaG007762 MtrunA17_Chr2g0295761 46.221 344 182 2 44 386 51 392 2.71e-102 308
MsaG007762 MtrunA17_Chr2g0295711 70.098 204 50 4 1 202 1 195 8.69e-90 269
MsaG007762 MtrunA17_Chr4g0067671 42.458 358 197 4 11 368 9 357 2.42e-89 274
MsaG007762 MtrunA17_Chr4g0067141 42.296 331 189 1 51 381 42 370 2.78e-89 274
MsaG007762 MtrunA17_Chr2g0286191 44.578 332 178 4 54 383 32 359 8.36e-87 267
MsaG007762 MtrunA17_Chr2g0296131 65.497 171 59 0 223 393 2 172 1.38e-77 237
MsaG007762 MtrunA17_Chr2g0296161 64.881 168 59 0 226 393 1 168 1.25e-76 234
MsaG007762 MtrunA17_Chr2g0296551 56.915 188 81 0 204 391 5 192 6.77e-73 226
MsaG007762 MtrunA17_Chr2g0296211 55.721 201 86 1 194 391 166 366 5.72e-71 227
MsaG007762 MtrunA17_Chr2g0295751 67.614 176 49 3 9 182 7 176 4.31e-67 220
MsaG007762 MtrunA17_Chr2g0296701 60.494 162 64 0 230 391 3 164 4.39e-66 207
MsaG007762 MtrunA17_Chr2g0295721 79.646 113 23 0 226 338 1 113 3.07e-57 183
MsaG007762 MtrunA17_Chr2g0296751 60.294 136 54 0 256 391 2 137 8.83e-54 176
MsaG007762 MtrunA17_Chr4g0001831 32.303 356 231 7 33 381 16 368 3.38e-46 162
MsaG007762 MtrunA17_Chr2g0286221 48.077 156 79 2 228 383 16 169 1.90e-40 141
MsaG007762 MtrunA17_Chr8g0377781 29.831 295 204 2 86 377 74 368 4.31e-34 130
MsaG007762 MtrunA17_Chr2g0296561 50.735 136 58 3 38 173 23 149 1.06e-33 122
MsaG007762 MtrunA17_Chr8g0377801 26.647 334 241 3 51 381 53 385 1.55e-28 115
MsaG007762 MtrunA17_Chr4g0067131 46.988 83 44 0 299 381 1 83 7.53e-21 86.3
MsaG007762 MtrunA17_Chr4g0067681 54.098 61 28 0 288 348 1 61 4.27e-17 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007762 AT5G07900.1 36.637 333 203 4 54 380 69 399 5.06e-69 223
MsaG007762 AT1G21150.1 30.816 331 221 4 54 378 56 384 2.74e-48 169
MsaG007762 AT1G21150.3 29.912 341 231 4 44 378 10 348 2.95e-48 167
MsaG007762 AT1G21150.2 30.816 331 221 4 54 378 120 448 1.09e-47 169
MsaG007762 AT1G61980.3 28.455 369 219 8 54 381 52 416 1.62e-34 132
MsaG007762 AT1G61980.2 28.455 369 219 8 54 381 52 416 1.62e-34 132
MsaG007762 AT1G61980.1 28.455 369 219 8 54 381 52 416 1.62e-34 132
MsaG007762 AT1G61970.2 26.685 356 208 7 55 366 53 399 1.37e-31 124
MsaG007762 AT1G61970.7 26.685 356 208 7 55 366 53 399 1.37e-31 124
MsaG007762 AT1G61970.6 26.685 356 208 7 55 366 53 399 1.37e-31 124
MsaG007762 AT1G61970.3 26.685 356 208 7 55 366 53 399 1.37e-31 124
MsaG007762 AT1G61970.5 26.685 356 208 7 55 366 53 399 1.37e-31 124
MsaG007762 AT1G61970.4 26.685 356 208 7 55 366 53 399 1.37e-31 124
MsaG007762 AT1G61970.1 26.685 356 208 7 55 366 53 399 1.37e-31 124
MsaG007762 AT1G62120.1 28.134 359 205 9 55 368 57 407 3.79e-31 123
MsaG007762 AT1G61990.2 27.324 355 206 7 55 368 53 396 1.24e-30 121
MsaG007762 AT1G61990.1 27.324 355 206 7 55 368 53 396 1.24e-30 121
MsaG007762 AT5G64950.1 28.616 318 209 5 82 388 65 375 2.16e-30 120
MsaG007762 AT1G62085.1 27.717 368 179 10 54 342 55 414 1.42e-28 116
MsaG007762 AT1G62085.2 27.717 368 179 10 54 342 55 414 1.42e-28 116
MsaG007762 AT3G46950.1 31.203 266 172 5 38 298 32 291 2.18e-27 113
MsaG007762 AT5G23930.1 27.110 391 203 10 54 371 55 436 1.83e-26 110
MsaG007762 AT1G62110.1 29.960 247 164 4 55 296 53 295 4.82e-23 100
MsaG007762 AT1G61960.1 28.794 257 167 4 48 296 46 294 1.18e-22 99.8
MsaG007762 AT1G62010.1 24.232 293 184 3 87 343 69 359 1.29e-21 96.3
MsaG007762 AT1G62150.1 24.185 368 192 9 55 343 59 418 1.79e-20 93.2

Find 85 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATACCTCATTCACAATTT+TGG 0.061494 2:+22635707 MsaT007762.1:CDS
GCAATCTGACAAGGAAATTT+TGG 0.125855 2:+22636048 MsaT007762.1:CDS
CGACCAACCCCATTTCTTAA+AGG 0.171385 2:-22636332 None:intergenic
GGAAAAGAGGGTTAGGGTTT+AGG 0.191466 2:-22635616 None:intergenic
GTGAAGGAATTAAAGGATTT+GGG 0.210460 2:+22636223 MsaT007762.1:CDS
GAAAAGAGGGTTAGGGTTTA+GGG 0.220235 2:-22635615 None:intergenic
GGTGAAGGAATTAAAGGATT+TGG 0.236355 2:+22636222 MsaT007762.1:CDS
TTAATTTAGTGATGAGCTTT+TGG 0.242008 2:+22636419 MsaT007762.1:CDS
TGACAAAATATCAGGTGTTT+TGG 0.255478 2:-22636476 None:intergenic
GTGCAATTTCTTCTCAATAA+TGG 0.259750 2:+22636538 MsaT007762.1:CDS
TAATCCCTTGAGATAGATTA+AGG 0.266032 2:-22635570 None:intergenic
CAAGTTGGTGAAGGAATTAA+AGG 0.289341 2:+22636216 MsaT007762.1:CDS
TTTCTCATTCCACAAGGTTT+TGG 0.304420 2:-22636302 None:intergenic
TTATCTTATAAAGTTGTATC+AGG 0.309342 2:+22636657 MsaT007762.1:CDS
TGAGAACTGAATCAGGGTTT+TGG 0.309396 2:-22635790 None:intergenic
CTTGTAACTGGAGCAAAGTT+TGG 0.321987 2:-22635750 None:intergenic
AAGAAATTGAGAACTGAATC+AGG 0.327189 2:-22635797 None:intergenic
AAACTTTGGTAACACCCTTT+TGG 0.336536 2:-22635897 None:intergenic
CAAAAGCGTTGGTGCAGATA+TGG 0.344003 2:-22635664 None:intergenic
CTTGAAACGGAGCTTGTAAC+TGG 0.364053 2:-22635762 None:intergenic
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2:+22636438 MsaT007762.1:CDS
AAGTTGATGCCTTTAAGAAA+TGG 0.366199 2:+22636323 MsaT007762.1:CDS
AGTTGATGCCTTTAAGAAAT+GGG 0.371481 2:+22636324 MsaT007762.1:CDS
AAACTTAATGACAAAATATC+AGG 0.378240 2:-22636484 None:intergenic
GCTTAATTTATCCATTCTTA+AGG 0.397953 2:+22636581 MsaT007762.1:CDS
CAAACATTAAAAGATTGCTT+AGG 0.402903 2:+22636158 MsaT007762.1:CDS
GTCAGAAGTAGAAGCGCCTT+TGG 0.405353 2:-22635933 None:intergenic
AATAGTCCCAACCTATGAAT+TGG 0.421560 2:+22636012 MsaT007762.1:CDS
ATATGGAGAGAAAAGAGAAA+TGG 0.422482 2:-22635647 None:intergenic
TGATGTTGGAAAAGAGGGTT+AGG 0.425634 2:-22635623 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.429067 2:+22636437 MsaT007762.1:CDS
GGAAGTAGCCATGGTGCCTT+GGG 0.435586 2:-22635863 None:intergenic
AAAAGAGAAATGGGTGATGT+TGG 0.437981 2:-22635637 None:intergenic
TTCCCTGCGACCCCACCAAA+AGG 0.439725 2:+22635882 MsaT007762.1:CDS
TATGGAGAGAAAAGAGAAAT+GGG 0.440569 2:-22635646 None:intergenic
GGGAAGTAGCCATGGTGCCT+TGG 0.446977 2:-22635864 None:intergenic
AAACATTAAAAGATTGCTTA+GGG 0.465201 2:+22636159 MsaT007762.1:CDS
CTCAATTTCTTTAGAAACCA+TGG 0.466986 2:+22635809 MsaT007762.1:CDS
GGGAATGATACGACGTAGTT+GGG 0.467688 2:-22635843 None:intergenic
AGGCTTACAGACAAGACGCC+AGG 0.474816 2:-22635980 None:intergenic
ATGGGTGATGTTGGAAAAGA+GGG 0.475318 2:-22635628 None:intergenic
GAATCTGTACACCAATTCAT+AGG 0.478001 2:-22636023 None:intergenic
CAAAGTTTGGAAGCAGATTG+AGG 0.480265 2:-22635737 None:intergenic
CTGTACACCAATTCATAGGT+TGG 0.484595 2:-22636019 None:intergenic
ATCACAATGCTGATTGAGAA+TGG 0.486666 2:+22636124 MsaT007762.1:CDS
TGGGAATGATACGACGTAGT+TGG 0.489187 2:-22635844 None:intergenic
GATTGCTTAGGGAACATACT+CGG 0.497604 2:+22636170 MsaT007762.1:CDS
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2:+22636447 MsaT007762.1:CDS
TGTACACCAATTCATAGGTT+GGG 0.507173 2:-22636018 None:intergenic
TTGGGACTATTTGATTCTTG+AGG 0.509547 2:-22636000 None:intergenic
AAAGTTTGGAAGCAGATTGA+GGG 0.518444 2:-22635736 None:intergenic
AACTTCTCAGACCTTAAGAA+TGG 0.523239 2:-22636592 None:intergenic
GGGTCGCAGGGAAGTAGCCA+TGG 0.525873 2:-22635872 None:intergenic
TCCCTGCGACCCCACCAAAA+GGG 0.532744 2:+22635883 MsaT007762.1:CDS
AACTCAAGCTTGCATACACC+AGG 0.537467 2:+22636683 MsaT007762.1:CDS
AATGGGTGATGTTGGAAAAG+AGG 0.538598 2:-22635629 None:intergenic
GAGGTATGAGACAGCAAAAG+AGG 0.548116 2:-22635693 None:intergenic
CGCCAGGGTTCTTACACACA+AGG 0.557000 2:-22635964 None:intergenic
ATTGCTTAGGGAACATACTC+GGG 0.568007 2:+22636171 MsaT007762.1:CDS
CGAGCCCAAAATTGTGAATG+AGG 0.569173 2:-22635712 None:intergenic
GTTGATGCCTTTAAGAAATG+GGG 0.573670 2:+22636325 MsaT007762.1:CDS
CAGATTCTTGCAATCTGACA+AGG 0.575407 2:+22636039 MsaT007762.1:CDS
TGTTCAAGTTCAACAATACA+TGG 0.576396 2:+22635537 MsaT007762.1:CDS
CAGAACCTTAATCTATCTCA+AGG 0.578893 2:+22635565 MsaT007762.1:CDS
AGAGGTTGAATCAAAAGCGT+TGG 0.582755 2:-22635675 None:intergenic
TGAAGGAATTAAAGGATTTG+GGG 0.584499 2:+22636224 MsaT007762.1:CDS
TGACAATCATGTCTTGTCCC+TGG 0.585106 2:-22636701 None:intergenic
GACACGAGACATGCTCAAGT+TGG 0.588113 2:+22636201 MsaT007762.1:CDS
AGAAATTGAGAACTGAATCA+GGG 0.588690 2:-22635796 None:intergenic
AATTGCACAACAGTGGCTCG+TGG 0.602758 2:-22636523 None:intergenic
ATGCCTTTAAGAAATGGGGT+TGG 0.606718 2:+22636329 MsaT007762.1:CDS
TGAGATAGATTAAGGTTCTG+TGG 0.615734 2:-22635562 None:intergenic
GTTGGGAGTTAGAGAAACCA+TGG 0.615746 2:-22635826 None:intergenic
CATCAACAACCAAAACCTTG+TGG 0.615914 2:+22636293 MsaT007762.1:CDS
AGACATGCTCAAGTTGGTGA+AGG 0.618045 2:+22636207 MsaT007762.1:CDS
GATGTTGGAAAAGAGGGTTA+GGG 0.618515 2:-22635622 None:intergenic
GGCTTACAGACAAGACGCCA+GGG 0.643701 2:-22635979 None:intergenic
ATCAACTTTCTCATTCCACA+AGG 0.645223 2:-22636308 None:intergenic
GAGAAGAAATTGCACAACAG+TGG 0.646434 2:-22636530 None:intergenic
AGAACCTTAATCTATCTCAA+GGG 0.657921 2:+22635566 MsaT007762.1:CDS
AACCTTGTGTGTAAGAACCC+TGG 0.659854 2:+22635962 MsaT007762.1:CDS
ACTACGTCGTATCATTCCCA+AGG 0.661929 2:+22635847 MsaT007762.1:CDS
GTATCATTCCCAAGGCACCA+TGG 0.712393 2:+22635855 MsaT007762.1:CDS
ATGGATGTTATCATACAGTG+AGG 0.724940 2:-22636388 None:intergenic
ACTCAAGCTTGCATACACCA+GGG 0.735651 2:+22636684 MsaT007762.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22635536 22636717 22635536 ID=MsaG007762
Chr2 mRNA 22635536 22636717 22635536 ID=MsaT007762.1;Parent=MsaG007762
Chr2 exon 22635536 22636717 22635536 ID=MsaT007762.1.exon1;Parent=MsaT007762.1
Chr2 CDS 22635536 22636717 22635536 ID=cds.MsaT007762.1;Parent=MsaT007762.1
Gene Sequence

>MsaT007762.1

ATGTTCAAGTTCAACAATACATGGCGCCACAGAACCTTAATCTATCTCAAGGGATTAACTTACCCCCAAAAATCACAATCCCTAAACCCTAACCCTCTTTTCCAACATCACCCATTTCTCTTTTCTCTCCATATCTGCACCAACGCTTTTGATTCAACCTCTTTTGCTGTCTCATACCTCATTCACAATTTTGGGCTCGACCCTCAATCTGCTTCCAAACTTTGCTCCAGTTACAAGCTCCGTTTCAAGACTCACCAAAACCCTGATTCAGTTCTCAATTTCTTTAGAAACCATGGTTTCTCTAACTCCCAACTACGTCGTATCATTCCCAAGGCACCATGGCTACTTCCCTGCGACCCCACCAAAAGGGTGTTACCAAAGTTTCAATTTTTTCTCTCCAAAGGCGCTTCTACTTCTGACATTGTTAACCTTGTGTGTAAGAACCCTGGCGTCTTGTCTGTAAGCCTCAAGAATCAAATAGTCCCAACCTATGAATTGGTGTACAGATTCTTGCAATCTGACAAGGAAATTTTGGCATTGTTGAATTACAATACAAATTTACTTTGTGATCGTACTGTGCATAATAACATCACAATGCTGATTGAGAATGGAGTGTCCCACTCAAACATTAAAAGATTGCTTAGGGAACATACTCGGGCATTCAAGACACGAGACATGCTCAAGTTGGTGAAGGAATTAAAGGATTTGGGGTTTAATCATTCAAAAACAACTTTTGGAGTAGCATTGATAGCAAAATCATCAACAACCAAAACCTTGTGGAATGAGAAAGTTGATGCCTTTAAGAAATGGGGTTGGTCGAATGAAGATGTTCTTCAAGCATTTAGAAAGCAGCCTCACTGTATGATAACATCCATAGATAAAATTAATTTAGTGATGAGCTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCCAAAACACCTGATATTTTGTCATTAAGTTTGGAAAAAAGGATCATTCCACGAGCCACTGTTGTGCAATTTCTTCTCAATAATGGTTTGCGAAGTAAGAATTCAAGCTTAATTTATCCATTCTTAAGGTCTGAGAAGTTGTTTCTTGATATGTTTATAAAACGTTTTAAGAATGAGTCTTCTTATCTTATAAAGTTGTATCAGGAAAAACTCAAGCTTGCATACACCAGGGACAAGACATGA

Protein sequence

>MsaT007762.1

MFKFNNTWRHRTLIYLKGLTYPQKSQSLNPNPLFQHHPFLFSLHICTNAFDSTSFAVSYLIHNFGLDPQSASKLCSSYKLRFKTHQNPDSVLNFFRNHGFSNSQLRRIIPKAPWLLPCDPTKRVLPKFQFFLSKGASTSDIVNLVCKNPGVLSVSLKNQIVPTYELVYRFLQSDKEILALLNYNTNLLCDRTVHNNITMLIENGVSHSNIKRLLREHTRAFKTRDMLKLVKELKDLGFNHSKTTFGVALIAKSSTTKTLWNEKVDAFKKWGWSNEDVLQAFRKQPHCMITSIDKINLVMSFWVNQLGWDAMAIAKTPDILSLSLEKRIIPRATVVQFLLNNGLRSKNSSLIYPFLRSEKLFLDMFIKRFKNESSYLIKLYQEKLKLAYTRDKT*