AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa ssp. caerulea / MsaG007763

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007763 MtrunA17_Chr2g0296151 94.764 382 20 0 1 382 1 382 0.0 743
MsaG007763 MtrunA17_Chr2g0296121 66.667 390 120 2 1 381 79 467 0.0 512
MsaG007763 MtrunA17_Chr2g0295731 68.280 372 116 1 5 374 10 381 1.44e-179 504
MsaG007763 MtrunA17_Chr2g0296111 65.455 385 121 4 3 382 5 382 1.94e-178 501
MsaG007763 MtrunA17_Chr2g0295741 65.928 361 123 0 19 379 25 385 2.53e-174 491
MsaG007763 MtrunA17_Chr2g0296251 48.622 399 162 5 13 370 2 398 3.07e-124 364
MsaG007763 MtrunA17_Chr2g0296711 52.663 338 157 1 42 376 55 392 1.97e-123 363
MsaG007763 MtrunA17_Chr2g0296221 51.955 358 163 3 32 380 67 424 6.50e-123 362
MsaG007763 MtrunA17_Chr2g0296721 51.240 363 168 3 26 380 31 392 2.60e-122 359
MsaG007763 MtrunA17_Chr2g0296231 51.811 359 165 5 26 380 36 390 1.10e-120 355
MsaG007763 MtrunA17_Chr2g0296741 49.733 374 170 4 24 380 41 413 2.73e-119 352
MsaG007763 MtrunA17_Chr2g0296301 48.451 355 177 3 24 374 18 370 9.61e-119 350
MsaG007763 MtrunA17_Chr4g0067121 51.014 345 166 2 36 379 47 389 1.69e-118 349
MsaG007763 MtrunA17_Chr2g0315731 49.451 364 174 3 26 380 51 413 1.93e-118 350
MsaG007763 MtrunA17_Chr2g0296291 46.829 410 173 6 13 380 2 408 3.49e-116 344
MsaG007763 MtrunA17_Chr2g0296171 51.075 372 161 5 26 383 37 401 9.13e-115 340
MsaG007763 MtrunA17_Chr2g0296191 51.567 351 160 4 27 367 644 994 1.93e-110 347
MsaG007763 MtrunA17_Chr2g0296191 48.767 365 181 3 5 369 151 509 3.09e-109 343
MsaG007763 MtrunA17_Chr2g0296181 48.732 355 151 4 26 376 36 363 3.02e-109 325
MsaG007763 MtrunA17_Chr2g0295761 44.947 376 191 3 12 373 19 392 1.78e-105 316
MsaG007763 MtrunA17_Chr4g0067141 44.315 343 187 2 30 370 32 372 2.33e-95 289
MsaG007763 MtrunA17_Chr2g0286191 46.687 332 171 4 41 370 32 359 1.96e-90 276
MsaG007763 MtrunA17_Chr4g0067671 44.099 322 178 1 34 355 38 357 9.04e-88 269
MsaG007763 MtrunA17_Chr2g0296161 62.941 170 63 0 213 382 1 170 2.09e-77 236
MsaG007763 MtrunA17_Chr2g0296131 62.941 170 63 0 210 379 2 171 1.33e-72 224
MsaG007763 MtrunA17_Chr2g0296551 54.737 190 86 0 191 380 5 194 6.40e-69 215
MsaG007763 MtrunA17_Chr2g0295751 67.222 180 50 4 1 173 1 178 2.33e-68 223
MsaG007763 MtrunA17_Chr2g0296211 48.571 245 109 2 153 380 124 368 9.31e-68 218
MsaG007763 MtrunA17_Chr2g0295711 58.824 187 75 1 5 189 9 195 3.95e-66 208
MsaG007763 MtrunA17_Chr2g0296701 58.750 160 66 0 217 376 3 162 3.68e-60 192
MsaG007763 MtrunA17_Chr2g0295721 76.991 113 26 0 213 325 1 113 1.52e-55 178
MsaG007763 MtrunA17_Chr2g0296751 60.294 136 54 0 243 378 2 137 3.28e-51 169
MsaG007763 MtrunA17_Chr4g0001831 29.189 370 244 6 5 368 11 368 3.00e-43 154
MsaG007763 MtrunA17_Chr2g0286221 50.641 156 75 2 215 370 16 169 3.11e-42 146
MsaG007763 MtrunA17_Chr8g0377781 29.341 334 230 4 35 364 37 368 2.24e-34 130
MsaG007763 MtrunA17_Chr2g0296561 53.333 120 52 2 25 144 23 138 8.94e-34 122
MsaG007763 MtrunA17_Chr8g0377801 27.331 311 211 3 67 368 81 385 1.20e-28 115
MsaG007763 MtrunA17_Chr4g0067131 49.383 81 41 0 286 366 1 81 1.12e-19 82.8
MsaG007763 MtrunA17_Chr4g0067681 55.738 61 27 0 275 335 1 61 1.19e-16 73.9
MsaG007763 MtrunA17_Chr8g0339041 21.753 308 206 7 80 368 57 348 1.01e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsaG007763 AT5G07900.1 34.234 333 211 5 41 367 69 399 4.07e-63 207
MsaG007763 AT1G21150.3 30.499 341 229 4 31 365 10 348 1.48e-51 176
MsaG007763 AT1G21150.1 31.420 331 219 4 41 365 56 384 1.96e-51 177
MsaG007763 AT1G21150.2 31.420 331 219 4 41 365 120 448 1.03e-50 176
MsaG007763 AT1G61980.3 28.000 375 211 9 41 367 52 415 3.63e-35 134
MsaG007763 AT1G61980.2 28.000 375 211 9 41 367 52 415 3.63e-35 134
MsaG007763 AT1G61980.1 28.000 375 211 9 41 367 52 415 3.63e-35 134
MsaG007763 AT5G64950.1 27.731 357 233 8 31 368 26 376 9.96e-33 127
MsaG007763 AT1G61970.2 28.045 353 207 8 42 353 53 399 3.45e-32 125
MsaG007763 AT1G61970.7 28.045 353 207 8 42 353 53 399 3.45e-32 125
MsaG007763 AT1G61970.6 28.045 353 207 8 42 353 53 399 3.45e-32 125
MsaG007763 AT1G61970.3 28.045 353 207 8 42 353 53 399 3.45e-32 125
MsaG007763 AT1G61970.5 28.045 353 207 8 42 353 53 399 3.45e-32 125
MsaG007763 AT1G61970.4 28.045 353 207 8 42 353 53 399 3.45e-32 125
MsaG007763 AT1G61970.1 28.045 353 207 8 42 353 53 399 3.45e-32 125
MsaG007763 AT1G61990.2 27.419 372 208 9 42 367 53 408 2.33e-30 120
MsaG007763 AT1G61990.1 27.419 372 208 9 42 367 53 408 2.33e-30 120
MsaG007763 AT3G46950.1 31.081 296 184 8 2 285 4 291 3.07e-30 120
MsaG007763 AT1G62085.1 27.536 414 211 13 2 329 4 414 6.60e-30 120
MsaG007763 AT1G62085.2 27.536 414 211 13 2 329 4 414 6.60e-30 120
MsaG007763 AT1G62120.1 28.267 329 192 6 42 330 57 381 7.08e-30 119
MsaG007763 AT5G23930.1 26.142 394 213 8 41 363 55 441 4.77e-27 112
MsaG007763 AT1G62010.1 26.421 299 177 5 71 330 65 359 1.52e-23 101
MsaG007763 AT1G62110.1 28.163 245 171 3 42 283 53 295 2.26e-23 101
MsaG007763 AT1G62150.1 23.306 369 208 7 35 330 52 418 5.79e-23 100
MsaG007763 AT1G61960.1 28.620 297 190 8 3 283 4 294 1.77e-22 99.0
MsaG007763 AT1G79220.1 24.430 307 191 8 79 380 119 389 1.91e-11 65.5

Find 79 sgRNAs with CRISPR-Local

Find sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATACCTCATCAACAATTT+TGG 0.080401 2:+22642456 MsaT007763.1:CDS
TGAAGGTGGTGGATGAATTA+AGG 0.202555 2:+22642964 MsaT007763.1:CDS
AAAGGGTTTGAGATTGAAAA+TGG 0.228978 2:-22642349 None:intergenic
CGACAAAATATCTGGTGTTT+TGG 0.229116 2:-22643225 None:intergenic
TTAATTTAGTGATGAGCTTT+TGG 0.242008 2:+22643168 MsaT007763.1:CDS
GAAAATGGTAATGGGGTTAT+TGG 0.243519 2:-22642387 None:intergenic
TTTCCTGCGACCCCTCTAAA+AGG 0.248958 2:+22642631 MsaT007763.1:CDS
GTGCAATTTCTTCTCAATAA+TGG 0.259750 2:+22643287 MsaT007763.1:CDS
GTGGATGAATTAAGGGATTT+GGG 0.278480 2:+22642972 MsaT007763.1:CDS
GGACCAACCCCACTTCTTAA+AGG 0.299701 2:-22643081 None:intergenic
TAGAGAAACCATGGTTTCTA+AGG 0.319966 2:-22642566 None:intergenic
GGTGGATGAATTAAGGGATT+TGG 0.320429 2:+22642971 MsaT007763.1:CDS
TTTCTCTTTCCACAAGGCTT+TGG 0.324915 2:-22643051 None:intergenic
TGAGATTGAAAATGGATCTA+TGG 0.333993 2:-22642341 None:intergenic
CGAGAACAGAATCAGGGTTT+TGG 0.341656 2:-22642539 None:intergenic
AATGAAGAGAAAATGGTAAT+GGG 0.355617 2:-22642395 None:intergenic
TATCGTTCCAATCTATGAAT+TGG 0.356154 2:+22642761 MsaT007763.1:CDS
GTGCAGAAATGAAGAGAAAA+TGG 0.357192 2:-22642402 None:intergenic
CAAATATTTCTAGATTGCTT+AGG 0.360863 2:+22642907 MsaT007763.1:CDS
GAAGGTGGTGGATGAATTAA+GGG 0.364635 2:+22642965 MsaT007763.1:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2:+22643187 MsaT007763.1:CDS
AAACTTAACGACAAAATATC+TGG 0.378028 2:-22643233 None:intergenic
AGGAAATCGAGAACAGAATC+AGG 0.380895 2:-22642546 None:intergenic
AAAATGGTAATGGGGTTATT+GGG 0.394325 2:-22642386 None:intergenic
GGGTATGGTGAGAAAGTAAA+GGG 0.406633 2:-22642366 None:intergenic
GTCAGAGGTAGACGCACCTT+TGG 0.411027 2:-22642682 None:intergenic
CTATAAACCAATTCATAGAT+TGG 0.415060 2:-22642768 None:intergenic
GAAAGTAGCCACGGTGCCTT+GGG 0.421775 2:-22642612 None:intergenic
GGAAAGTAGCCACGGTGCCT+TGG 0.424368 2:-22642613 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.429067 2:+22643186 MsaT007763.1:CDS
AAATATTTCTAGATTGCTTA+GGG 0.430507 2:+22642908 MsaT007763.1:CDS
ATTACATTGTTGCTTCAGAA+TGG 0.447044 2:+22642873 MsaT007763.1:CDS
AAGCTTACAGACAAGATCCT+AGG 0.448203 2:-22642729 None:intergenic
AATTGCACAACAGTGGCTCT+TGG 0.457636 2:-22643272 None:intergenic
GGGAATGATACGACGTAGTT+GGG 0.467688 2:-22642592 None:intergenic
AGATGGTCACTAAATAAAAC+AGG 0.477887 2:-22642837 None:intergenic
AAATGAAGAGAAAATGGTAA+TGG 0.484200 2:-22642396 None:intergenic
TGGGAATGATACGACGTAGT+TGG 0.489187 2:-22642593 None:intergenic
AACTTCTCAGGCATTAAGAA+TGG 0.491818 2:-22643341 None:intergenic
GTATCAAGAAATAACTTCTC+AGG 0.495468 2:-22643353 None:intergenic
TGGGTATGGTGAGAAAGTAA+AGG 0.497840 2:-22642367 None:intergenic
GGTAATGGGGTTATTGGGTA+TGG 0.511895 2:-22642381 None:intergenic
CTCGATTTCCTTAGAAACCA+TGG 0.516905 2:+22642558 MsaT007763.1:CDS
TTTATTTAGTGACCATCTTG+TGG 0.528406 2:+22642842 MsaT007763.1:CDS
CAAAGCTTAGAAGCAAATTG+TGG 0.530033 2:-22642486 None:intergenic
ATGCCTTTAAGAAGTGGGGT+TGG 0.530413 2:+22643078 MsaT007763.1:CDS
TCAGTTGGGTTGGGATGCTA+AGG 0.533835 2:+22643196 MsaT007763.1:CDS
AGTTGATGCCTTTAAGAAGT+GGG 0.541443 2:+22643073 MsaT007763.1:CDS
ACTTTATGAGTAAGAACCCT+AGG 0.541465 2:+22642712 MsaT007763.1:CDS
TTCCTGCGACCCCTCTAAAA+GGG 0.542437 2:+22642632 MsaT007763.1:CDS
CTTGAAACGGAGCTTGTAAG+TGG 0.546088 2:-22642511 None:intergenic
CAACTTTAAAAGATTAGAAG+CGG 0.547155 2:-22643399 None:intergenic
AAGTTGATGCCTTTAAGAAG+TGG 0.551383 2:+22643072 MsaT007763.1:CDS
AAAGCTTAGAAGCAAATTGT+GGG 0.556435 2:-22642485 None:intergenic
AGCTTACAGACAAGATCCTA+GGG 0.560835 2:-22642728 None:intergenic
CAAACCCAAAATTGTTGATG+AGG 0.572702 2:-22642461 None:intergenic
TAGATTCTTGCAATCGCACA+AGG 0.582615 2:+22642788 MsaT007763.1:CDS
TGTTCGTGGTGTATGCAAGA+TGG 0.584185 2:-22643436 None:intergenic
CATCAACAACCAAAGCCTTG+TGG 0.591454 2:+22643042 MsaT007763.1:CDS
CAATCTAGAAATATTTGAGT+CGG 0.591851 2:-22642901 None:intergenic
ATGAAGAGAAAATGGTAATG+GGG 0.595101 2:-22642394 None:intergenic
TGCTTCAAGAACATCTTCAT+CGG 0.599346 2:-22643102 None:intergenic
GAGGTATGAAACTGCAAATG+AGG 0.601875 2:-22642442 None:intergenic
ATCAACTTTCTCTTTCCACA+AGG 0.604525 2:-22643057 None:intergenic
TGGATGAATTAAGGGATTTG+GGG 0.606794 2:+22642973 MsaT007763.1:CDS
TAATGTTATGTGCCACAAGA+TGG 0.610046 2:-22642854 None:intergenic
CACATGTGTGCAATGTCCTA+CGG 0.613554 2:-22642935 None:intergenic
GTTGATGCCTTTAAGAAGTG+GGG 0.614341 2:+22643074 MsaT007763.1:CDS
GGAAATCGAGAACAGAATCA+GGG 0.615688 2:-22642545 None:intergenic
GTTGGGAGTTAGAGAAACCA+TGG 0.615746 2:-22642575 None:intergenic
GGGTCGCAGGAAAGTAGCCA+CGG 0.625027 2:-22642621 None:intergenic
TGAGGTTGAATCTGAAGTGT+TGG 0.626308 2:-22642424 None:intergenic
CATAAAGTTAACCATGTCAG+AGG 0.632724 2:-22642697 None:intergenic
GAGAAGAAATTGCACAACAG+TGG 0.646434 2:-22643279 None:intergenic
ATGGATGTTAACATACAATG+AGG 0.655070 2:-22643137 None:intergenic
ACTACGTCGTATCATTCCCA+AGG 0.661929 2:+22642596 MsaT007763.1:CDS
AGGTGCGTCTACCTCTGACA+TGG 0.678048 2:+22642686 MsaT007763.1:CDS
GATTGCTTAGGGAACACCGT+AGG 0.718101 2:+22642919 MsaT007763.1:CDS
GTATCATTCCCAAGGCACCG+TGG 0.787154 2:+22642604 MsaT007763.1:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
Chromosome Type Strat End Strand Name
Chr2 gene 22642324 22643475 22642324 ID=MsaG007763
Chr2 mRNA 22642324 22643475 22642324 ID=MsaT007763.1;Parent=MsaG007763
Chr2 exon 22642324 22643475 22642324 ID=MsaT007763.1.exon1;Parent=MsaT007763.1
Chr2 CDS 22642324 22643475 22642324 ID=cds.MsaT007763.1;Parent=MsaT007763.1
Gene Sequence

>MsaT007763.1

ATGTTGATTATTCATCGCCATAGATCCATTTTCAATCTCAAACCCTTTACTTTCTCACCATACCCAATAACCCCATTACCATTTTCTCTTCATTTCTGCACCAACACTTCAGATTCAACCTCATTTGCAGTTTCATACCTCATCAACAATTTTGGGTTTGACCCACAATTTGCTTCTAAGCTTTGCTCCACTTACAAGCTCCGTTTCAAGACTGACCAAAACCCTGATTCTGTTCTCGATTTCCTTAGAAACCATGGTTTCTCTAACTCCCAACTACGTCGTATCATTCCCAAGGCACCGTGGCTACTTTCCTGCGACCCCTCTAAAAGGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCGTCTACCTCTGACATGGTTAACTTTATGAGTAAGAACCCTAGGATCTTGTCTGTAAGCTTGAAGAATCGTATCGTTCCAATCTATGAATTGGTTTATAGATTCTTGCAATCGCACAAGGACGCTATTGCTTGTGTAATTAGCAATCCTGTTTTATTTAGTGACCATCTTGTGGCACATAACATTACATTGTTGCTTCAGAATGGACTCTCCGACTCAAATATTTCTAGATTGCTTAGGGAACACCGTAGGACATTGCACACATGTGACATTTTGAAGGTGGTGGATGAATTAAGGGATTTGGGGTTTAATCCTTCAAAAACAACTTTTGGTGTAGCTTTGATAGCGAAATCATCAACAACCAAAGCCTTGTGGAAAGAGAAAGTTGATGCCTTTAAGAAGTGGGGTTGGTCCGATGAAGATGTTCTTGAAGCATTTAAAAAGCAACCTCATTGTATGTTAACATCCATTGATAAAATTAATTTAGTGATGAGCTTTTGGGTCAATCAGTTGGGTTGGGATGCTAAGGACATTGCCAAAACACCAGATATTTTGTCGTTAAGTTTGGAAAAAAGGATCATTCCAAGAGCCACTGTTGTGCAATTTCTTCTCAATAATGGTTTGCGAAATAAGAATTCAAGCTTAATTTATCCATTCTTAATGCCTGAGAAGTTATTTCTTGATACGTTTGTAAAACGTTTTGAGAATGCCGCTTCTAATCTTTTAAAGTTGTATGAAGAAAAACTCCATCTTGCATACACCACGAACAAAAATTGCACGAAATGA
Protein sequence

>MsaT007763.1

MLIIHRHRSIFNLKPFTFSPYPITPLPFSLHFCTNTSDSTSFAVSYLINNFGFDPQFASKLCSTYKLRFKTDQNPDSVLDFLRNHGFSNSQLRRIIPKAPWLLSCDPSKRVLPKFQFFLSKGASTSDMVNFMSKNPRILSVSLKNRIVPIYELVYRFLQSHKDAIACVISNPVLFSDHLVAHNITLLLQNGLSDSNISRLLREHRRTLHTCDILKVVDELRDLGFNPSKTTFGVALIAKSSTTKALWKEKVDAFKKWGWSDEDVLEAFKKQPHCMLTSIDKINLVMSFWVNQLGWDAKDIAKTPDILSLSLEKRIIPRATVVQFLLNNGLRNKNSSLIYPFLMPEKLFLDTFVKRFENAASNLLKLYEEKLHLAYTTNKNCTK*